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Odovtos - International Journal of Dental Sciences
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 1659-1046 - ISSN (Online) 2215-3411
Published by Universidad de Costa Rica Homepage  [39 journals]
  • Open Access and Bibliometric Data: Odovtos-Int.J.Dent.Sc.

    • Authors: Jessie Reyes-Carmona
      Pages: 8 - 9
      Abstract: .
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.54931
      Issue No: Vol. 25, No. 2 (2023)
  • Assessment of Electromyography in Evaluating Masseter Muscle Activity in
           Oral Submucous Fibrosis with Different Treatment Modalities: a Systematic

    • Authors: Poornachitra P., Uma Maheswari T.N.
      Pages: 10 - 19
      Abstract: Electromyography (EMG) is used for the measurement of muscle activity to characterize the nature of muscle contraction in Oral submucous fibrosis (OSMF). Aim:  To assess the efficacy of EMG in evaluating masseter muscle activity in the management of OSMF. This review identified 73 records from standard databases which were rigorously screened with eligibility criteria and 3 clinical studies were identified based on our inclusion criteria. The quality of included studies was assessed by the PEDro scale and data was synthesized with detailed characterization. The Risk of Bias assessment among studies was done using the ROBINS-I tool and a meta-analysis could not be done due to high clinical heterogeneity. Our result recommends that EMG be used as an objective prognosis assessment tool by quantifying the management of OSMF irrespective of the intervention applied. However, it is not to be considered the gold standard as of now with limited data pooled and needs to be further assessed with clinical trials.  EMG can be advocated as a reliable adjunct assessment for measuring the interventional outcome of OSMF irrespective of treatment modalities.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.54119
      Issue No: Vol. 25, No. 2 (2023)
  • Effects of Whitening Dentifrices on the Enamel Color, Surface Roughness,
           and Morphology

    • Authors: Ahmet Hazar, Ecehan Hazar
      Pages: 20 - 29
      Abstract: The aim of this study was to evaluate the whitening and abrasion effects of dentifrices containing different whitening agents on artificially stained and unstained enamel. 160 human dental enamel discs were distributed into four groups according to the type of dentifrices applied on them (n=40): Group I, non-whitening dentifrice (control group); Group II, dentifrice containing charcoal; Group III, dentifrice containing blue covarine; Group IV, dentifrice containing hydrogen peroxide. Half of the specimens in each group were immersed in coffee solution. Color measurements were evaluated from stained and unstained specimens before and after brushing by a spectrophotometer. The surface roughness of each unstained specimen was analyzed using a profilometer after the brushing and bleaching. The surface of one randomly selected specimen from each group was observed using a scanning electron microscope (SEM). Statistical analysis of the color change was performed using the Kruskal-Wallis test. The one-way ANOVA was used to evaluate surface roughness. Group III provided significantly higher recovery on ∆E00 values than other groups on the unstained enamel (p<0.05). Group IV showed significantly the highest ∆E00 values on stained enamel (p<0.05) and also Group IV showed the highest surface roughness values (p<0.05), SEM revealed a more irregular surface in groups III and IV. It can be stated that dentifrice containing blue covarine is both an effective and a safe way to provide whiter teeth with routine home tooth brushing.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.50981
      Issue No: Vol. 25, No. 2 (2023)
  • Effect of Different Obturation Techniques on Sealer Penetration into
           Dentinal Tubules after Final Irrigation with XP-Endo Finisher File

    • Authors: Erhan Demir, Selin Göker Kamalı, Dilek Türkaydın
      Pages: 30 - 39
      Abstract: The first objective of this research was to evaluate the effectiveness of XP-Endo Finisher on dentinal tubule penetration of irrigation solution using confocal laser scanning microscopy. The main purpose of this research was to compare the effect of cold lateral condensation, continuous wave obturation and core-carrier based techniques on sealer penetration. Sixty mandibular premolars were prepared and allocated into two experimental groups (n=30) as the final irrigation technique and obturation technique experiment. In the final irrigation technique experiment, final irrigation was performed with XP-Endo Finisher, passive ultrasonic irrigation (PUI) and conventional needle irrigation (CNI) (n=10). The roots in the obturation technique experiment were also assigned into 3 groups and obturated with cold lateral condensation, continuous-wave obturation and core-carrier techniques (n=10). The most effective activation method, which emerged as a result of the first part of this study, was used as the final irrigation method in the obturation technique experiment. Then, all roots were sectioned in 1-mm-thick slices at 3mm from the apex for scanning. In terms of depth and percentage of material penetration, CNI exhibited significantly the lowest values and no significant difference was found between others. Also, there was no significant difference among obturation methods. In conclusion, XP-Endo Finisher and PUI are more effective than CNI on irrigant penetration. Sealer penetration into dentinal tubules is independent of obturation techniques.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.51699
      Issue No: Vol. 25, No. 2 (2023)
  • The Effect of Whitening Toothpastes on Colour Change and Surface Roughness
           of Restorative Materials

    • Authors: Burak Dayi, Fikri Öcal
      Pages: 40 - 52
      Abstract: To examine the colour change and surface roughness of giomer, microhybrid composite and nanohybrid composite after brushing with whitening toothpastes. Disc-shaped samples of giomer, microhybrid composite and nanohybrid composite were divided into three subgroups(n=10), with the initial colour measured with a spectrophotometer and the surface roughness measured with a mechanical profilometer. The samples, which were immersed in coffee solution were brushed once a day with whitening toothpastes (Opalescence Cool Mint (OCM), Colgate Optic White Extra Power (COW) and Signal White Now Gold (SWN)) for 12 days. Colour change and surface roughness were measured again after the colouring-brushing cycle. All of the analyses were performed using appropriate statistical hypothesis tests. The highest increase in surface roughness was seen in the microhybrid composite group applied with SWN (p<0.05). The group with the least increase was the giomer group applied with SWN (p<0.05). While the least colour change was observed in the COW applied nanohybrid composite group (∆E00=1,814), the most colour change occurred in the COW applied giomer group (∆E00=5.943). After the use of whitening toothpastes, the roughness of giomer, microhybrid composite and nanohybrid composite surfaces was increased and a colour change above the clinically accepted value was observed.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.52728
      Issue No: Vol. 25, No. 2 (2023)
  • Elimination of E. faecalis and C. albicans Biofilm: A Comparison Between
           Single and Multiple File Systems in an Ex Vivo Study

    • Authors: Verónica Méndez-González, Claudia Casillas-Contreras, Marlen Vitales-Noyola, Diana Lorena Alvarado-Hernández, Ana María González-Amaro, Antonio Aragón-Piña, Amaury Pozos-Guillén
      Pages: 53 - 64
      Abstract: To evaluate whether the WaveOne Gold and Reciproc single file instrumentation systems, are effective in reducing the microbial load of a mixed biofilm and the cleaning of apical third compared to the Twisted File Adaptive system (multiple-file system). Seventy mesial roots of the first and second molars were included and randomly divided into three experimental groups (n=20, n=10 controls). Biofilms were formed inside canals over 31 days. After instrumentation with the unique file systems, WaveOne Gold and Reciproc and the multiple file system Twisted File Adaptive, using 2.25% sodium hypochlorite as an irrigant in all cases, a count of colony forming units was performed using serial dilutions, cleaning of the apical third was evaluated using scanning electron microscopy. Comparisons amongst groups were made by using parametric and non-parametric statistics, according to a normal or non-normal data distribution, respectively.  No significant differences in the reduction of the microbial load after employing a single-file system in comparison to the multiple-file system were found; in addition, the cleaning of the apical third was similar for the three different instrumentation systems. The single-file system is equal in effectiveness compared with the multiple-file system in reducing the microbial load.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.53153
      Issue No: Vol. 25, No. 2 (2023)
  • Effect of Dental Distance from Implant and MAR Algorithm on the Detection
           of Vertical Root Fracture in CBCT Images

    • Authors: Fatemeh Salemi
      Pages: 65 - 77
      Abstract: Three-dimensional cone-beam computed tomography (CBCT) has an important role in the detection of vertical root fractures (VRFs). The effect of artifact generation by high-density objects like dental implants on image quality was well documented. This study aimed to assess the effect of tooth-implant distance and the application of metal artifact reduction (MAR) algorithm on the detection of VRFs on CBCT scans. This study was conducted on 20 endodontically treated single-rooted teeth. VRFs were induced in 10 teeth, while the other 10 remained intact. The implant was inserted in the right second premolar socket area, and two teeth were inserted in right canine and right first premolar sockets area randomly and underwent CBCT with and without the application of MAR algorithm. SPSS 21 was used to analyze the results (alpha=0.05). According to the findings of this study, all four variables of sensitivity, specificity, accuracy, and positive predictive values in diagnosis were higher in cases without MAR software at both close(roots in first premolar sockets) and far distances (roots in canine sockets) from the implant. However, the highest rate of diagnosis accuracy of the first and second radiologists was in the far distance group from the implant without MAR, and the lowest rate of diagnosis accuracy in the first and second radiologists was in the close distance to the implant. Applying MAR algorithm had no positive effect on detection of VRFs on CBCT scans in both close and distant scenarios.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.53751
      Issue No: Vol. 25, No. 2 (2023)
  • Autophagic-Related Anticancer Effect of Grapes Extract and Tomatoes
           Extract: Ex-Vivo Study

    • Authors: Marwa M. Elshafei, Iman M. Helmy, Marwa M. Sayed, Doaa B. Farag, Ismail M. Shebl, Shaimaa E. Ghazy, Nermeen S. Afifi
      Pages: 78 - 92
      Abstract: Cells undergo autophagy to save themselves from injury, but progressive autophagy can cause cell death. This study characterized and compared the effect of grape (resveratrol) and tomato (lycopene) extracts and their combination on modulating autophagy-related miRNA and its target gene in squamous cell carcinoma cell line. Docking analysis for extracts and selected genes was performed. Methyl Thiazol Tetrazolium assays were used to assess the cytotoxicity of extracts and their combination toward HEp-2 cells. qRT-PCR was used to quantify changes in gene expression. Data were statistically analyzed. miRNA-20a was identified as a potential effector in laryngeal cancer, and sequestosome-1 (SQSTM1) was its target gene. Docking analysis showed that resveratrol interacted with miRNA-20a and showed less affinity toward SQSTM1. Hydrogen bonds and hydrophobic interactions were predicted. In contrast, lycopene showed less affinity toward miRNA-20a than resveratrol. Increasing doses of resveratrol, lycopene, and their combination induced a statistically significant reduction in mean percent viability and mean fold changes of miRNA-20a and SQSTM1 expression in treated HEp-2 cells. Pearson’s correlation showed a statistically significant positive correlation between miRNA-20a and SQSTM1 (R=0.812, p≤0.001). Grape and tomato extracts and their combination display promising cytotoxicity against HEp-2 cells in a dose- and time-dependent fashion. Both extracts reduce the expression of miRNA-20a and SQSTM1 with subsequent inhibition autophagy and promotion of apoptosis in HEp-2 cells.  
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.53752
      Issue No: Vol. 25, No. 2 (2023)
  • Effect of Voxel Size of Micro-CT on the Assessment of Root Canal

    • Authors: Jader Camilo Pinto, Everton Lucas-Oliveira, Tito Jose Bonagamba, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho
      Pages: 93 - 102
      Abstract: The aim of this study was to assess the influence of micro-computed tomography (micro-CT) voxel size on evaluation of root canal preparation using rotary heat-treated nickel-titanium files. Curved mesial root canals of mandibular molars were prepared using ProDesign Logic 30/.05 (PDL) or HyFlex EDM 25/.08 (HEDM) (n=12). The specimens were scanned using micro-CT with 5μm of voxel size before and after root canal preparation. Images with sub-resolution of 10 and 20μm voxel sizes were obtained. The percentage of volume increase, debris and uninstrumented root canal surface were analyzed in the different voxel sizes. Data were compared using unpaired Student’s t-test and ANOVA statistical tests (α=0.05). No differences were observed for percentage of volume increase, debris and instrumented surface between the root canals prepared by PDL and HEDM (p>0.05).  Both systems promoted higher percentage of debris in the apical third compared to the middle third (p<0.05). After instrumentation using PDL the percentage of uninstrumented surface was highr in the apical third than middle third only when analysis were performed at 5µm (p<0.05). When comparing the different voxel sizes (5,10 or 20µm), both groups showed different means for the variables, with no significant difference (p>0.05). PDL and HEDM had similar root canal preparation capacity. Micro-CT images using different voxel sizes did not influence the results of volume increase and debris evaluation. However, images at 5µm showed greater accuracy to evaluate the percentage of uninstrumented surfaces.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.53811
      Issue No: Vol. 25, No. 2 (2023)
  • Immunoexpression of ALDH1A1, FGFR2, CD44 and Caspase-3 in Oral Squamous
           Cell Carcinoma and Leukoplakia: A Pilot Study

    • Authors: Yadira V. Boza Oreamuno, Jessie F. Reyes-Carmona
      Pages: 103 - 111
      Abstract: Current research highlighted the importance to recognize feasible biomarkers for early diagnoses and treatment in oral cancer. Our study analyzed the expression and spatial distribution of ALDH1A1, FGFR2, caspase-3, and CD44 in Oral Squamous Cell Carcinoma (OSCC) and leukoplakia with and without oral mucosal dysplasia. Paraffin-embedded samples of OSCC (n=5), leukoplakia with (n=5) and without (n=5) dysplasia obtained by incisional biopsies were processed using conventional histochemical techniques. Immunohistochemistry was performed using antibodies against ALDH1A1, FGFR2, caspase-3, and CD44. Images of the immunohistochemically stained tissue sections were analyzed according to the intensity of the immunostaining of each marker and classified in Scores. The Kruskal-Wallis test was performed (p≤0.05). Our results demonstrated a statically difference in the expression of all immunomarkers between OSCC and leukoplakia without dysplasia, being more significant in FGFR2 and ALDH1A1. Within the limitations of this study, our data showed that all biomarkers were overexpressed in OSCC and leukoplakia with oral mucosa dysplasia, suggesting that the presence of dysplasia is a significant clinic-pathologic predictor for malignant transformation.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.50369
      Issue No: Vol. 25, No. 2 (2023)
  • 3D Printing Characteristics and Mechanical Properties of a Bio Scaffold
           Obtained from a Micro-CT Scan, Using the Fused Deposition Modeling

    • Authors: Natalia González-Sánchez, Nicole Jensen-Líos, Diana Hernández-Montoya, José Esteban Campos Zumbado, Jorge Oviedo-Quirós
      Pages: 112 - 122
      Abstract: The objective is to determine which biopolymer has the best 3D printing characteristics and mechanical properties for the manufacture of a bioscaffold, using the fused deposition printing technique, with models generated from an STL file obtained from a Micro-CT scan taken from a bovine iliac crest bone structure. Through an experimental exploratory study, three study groups of the analyzed biopolymers were carried out with thirteen printed structures of each one. The first is made of 100% PLA. The second, 90B, we added 1g of diatom extract, and the third, 88C, differs from the previous one in that it also contains 1g of calcium phosphate. The 39 printed structures underwent a visual inspection test, which required the fabrication of a gold standard scaffold in resin, with greater detail and similarity to the scanned bone structure. Finally, the structures were subjected to a compressive force (N) to obtain the modulus of elasticity (MPa) and compressive strength (MPa) of each one of them. A statistically significant difference (p=0.001) was obtained in the printing properties of the biomaterial 88C, compared to 90B and pure PLA and the 88C presented the best 3D printing characteristics. In addition, it also presented the best mechanical properties compared to the other groups of materials. Although the difference between these was not statistically significant (p=0.388), in the structures of the 88C biomaterial, values of compressive strength (8,84692 MPa) and modulus of elasticity (43,23615 MPa) were similar to those of cancellous bone in the jaws could be observed. Because of this result, the 88C biomaterial has the potential to be used in the manufacture of bioscaffolds in tissue engineering.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.52671
      Issue No: Vol. 25, No. 2 (2023)
  • Methylation Analysis of Rb, P16, MGMT, and hMLH-1in Mucoepidermoid
           Carcinomas. A Pilot Study

    • Authors: Luis A. Martínez-Marcial, Luis F. Jacinto-Alemán, David A. Trejo-Remigio, Carla M. Ramírez-Martínez, Elba R. Leyva-Huerta
      Pages: 123 - 132
      Abstract: Mucoepidermoid carcinoma (MC) is the most common malignant epithelial neoplasm in the salivary glands. This neoplasm has varying proportions of mucous, epidermoid, intermediate, columnar, and clear cells. MCs have been associated with CRTC1-MAML2 genes; however, their pathogenesis is uncertain. Recently, epigenetic changes have been considered a possible aetiologic factor. To identify the methylation state of RB, P16, MGMT, and hMLH genes in the three severity grades of MC were used five MCs and one healthy minor salivary gland as a control group (CG) obtained from the Pathology and Oral Medicine Laboratory and analyzed using MS-PCR to compare the presence or absence of methylation in promotor regions. The Kruskal-Wallis test was performed, with p≤0.05 considered significant. CG was employed as the normalizer of methylation levels. All assays were performed in triplicate. The mean age of our population was 52.6±18.6 years old; the total population was female and included 2 low grade, 2 intermediate grade, and 1 high grade levels of severity. When comparing the methylation status of the three histopathological grades of MC against the control, statistically significant differences were observed in Rb-M, MGMT-M, and hMLH-1-NM for high-grade severity, with p values of 0.03, 0.05, and 0.04, respectively. Methylation is a possible mechanism for pathogenesis processing of high-grade MC. However, a larger sample population is necessary to validate this finding.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2022.53197
      Issue No: Vol. 25, No. 2 (2023)
  • Immunological and Bacteriological Monitoring of Periodontal Tissue in

    • Authors: Natalia Solari, Leonardo Salas, Sandra Tabares, Carolina Rosella, María Matilde Usin, Adela Sembaj
      Pages: 133 - 143
      Abstract: We determine periodontal pathogens in periodontal pockets from pregnant women with periodontitis and associate it to the C reactive protein (CRP), nitrates, immunoglobulin A and G (Ig A and G), and myeloperoxidase (MPO) levels in saliva to identify some biomarkers as tools to predict the periodontal status from pregnant. The samples were obtained from periodontal pockets and saliva from 100 pregnant women (PW) and 50 non-pregnant women (NPW). Every patient was evaluated by: 1) probing depth (PD) and loss of clinical attachment level (CAL); 2) in saliva; CRP, MPO, Ig A and G) and nitrite concentrations, 3) in periodontal pockets: P.gingivalis, T.forsythia, T.denticola, P.intermedia, A.actinomycetemcomitans. InfoStat/P 2008 software was used with a p-value <0.05. Clinical parameters showed stages I and II of PD in both groups. P.intermedia and A.actinomycetemcomitans were observed only in periodontal pockets from PW. The CAL was higher in pregnant of the 3rd trimester than in the other stages and was associated with low levels of IgA and the presence of P.intermedia and T. forsythia in the same trimester. The levels of IgA in saliva would reflect the immunological situation in pregnant women. This could be used to monitor the immune status of the gingival tissues during pregnancy.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.54127
      Issue No: Vol. 25, No. 2 (2023)
  • CBCT Study of Root Apices Distance to Mandibular Canal in Peruvian

    • Authors: Paola Manrique, Andrés Agurto, María Eugenia Guerrero
      Pages: 144 - 154
      Abstract: The purpose of this study was to compare the average distances from the root apices of the first molars, second molars, and second premolars to the mandibular canal according to sex in the Peruvian population using cone-beam computed tomography (CBCT). Eighty CBCT scans of Peruvian patients aged from 15-80 years were examined. After locating the mandibular canal, measurements of the vertical distances from the mandibular canal to the apices of the second premolars, as well as the first molars and second molars, were made. For the statistical analysis, Student’s t test was used for both paired and unpaired samples, with a significance level of p<0.05. On the right side, the second molar presented a mean distance of 3.99mm for males and 2.87mm for females, showing a significant difference (p<0.05). When compared bilaterally, no significant differences were found (p>0.05) between the distances from the apices of the second premolars and the first and second molars to the mandibular canal. However, for the second premolars and second molars on the left side, the values were higher, with averages of 5.52mm and 3.75mm, respectively.The mesial roots of the second molars were closer to the mandibular canal. In addition, women showed shorter distances than men.
      PubDate: 2023-04-26
      DOI: 10.15517/ijds.2023.54120
      Issue No: Vol. 25, No. 2 (2023)
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Heriot-Watt University
Edinburgh, EH14 4AS, UK
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