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Journal of Aquaculture and Fish Health
Number of Followers: 1  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2301-7309 - ISSN (Online) 2528-0864
Published by Universitas Airlangga Homepage  [54 journals]
  • Effect of Spirulina (Arthrospira platensis) Powder on The Physico-chemical
           and Sensory Characterization of Dry Noodle

    • Authors: Dinda Ersyah, Abdul Aziz Jaziri, Dwi Setijawati
      Pages: 277 - 288
      Abstract: Dry noodles are a staple food item among Asian population. Generally, it contains a low-fat and a high-carbohydrate level, however it lacks other nutrients, such as protein and dietary fiber. The addition of spirulina into dry noodles may increase both protein and dietary fiber. The aim of this study was to determine the effect of spirulina fortified in the dry noodle on physico-chemical and sensory properties. The method used in this study was experimental with a completely randomized design (CRD) using 4 replications. The spirulina powders added into dry noodles were 0%, 4%, 4.5%, 5%, 5.5%, 6% and 6.5%. The results showed the addition of spirulina in dry noodles had a significant effect (p<0.05) on nutritional values, such as protein, fat, ash, carbohydrate and dietary fiber. In addition, physical properties of fortified noodles, including cooking loss, elongation and tensile strength were also significant differences. For sensory attributes, noodles fortified with different concentrations of spirulina showed significant effects in appearance, aroma, color, taste and texture. A selected dry noodle fortified with 6% of spirulina based on the physico-chemical and sensory characterization. The fortified dry noodle can contribute to improving nutritional profile that may be beneficial on health.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.20908
      Issue No: Vol. 11, No. 3 (2022)
  • Effect of Feeding Fermented Leucaena leucocephala Seed Meal on Growth and
           Protein Content of Osphronemus gouramy Juvenile

    • Authors: Putri Dista Ananda, Safrida Safrida, Khairil Khairil, Hasanuddin Hasanuddin , Asiah M.D.
      Pages: 289 - 297
      Abstract: Gouramy (Osphronemus gouramy Lac.) is one of the original fish from Indonesia that lives in fresh water and has high economic value because of its dense texture, high nutrition, and delicious taste so that it is widely favored by Indonesians. The results of juvenile cultivation activities have not been going well so far, this is because the growth of gouramy’s juvenile is slower than the growth of other freshwater fish’s juvenile. One alternative that can increase the growth and protein content of gouramy juvenile is to utilize of the availability of lamtoro seeds. The purpose of this study was to determine the effect of feeding lamtoro seed fermentation on growth and protein content of gouramy’s juvenile. This study used experimental method with a completely randomized design (CRD with 6 treatments and 4 replications). Data were analyzed using Analysis of Variance Test (ANOVA) and further test in the form of Honest Significant Difference (Tukey HSD Test) at the 95% confidence level. The results showed that P3 with the addition of fermented lamtoro seed flour 40% can increase the growth and protein content of gouramy juvenile. The conclusion of this study was lamtoro seed fermented feed has effect in increasing growth and protein content of gouramy’s juvenile.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.25879
      Issue No: Vol. 11, No. 3 (2022)
  • Morphometric and Meristic Analysis of Rasbora in East Java Province

    • Authors: P Kumaladewi, Mufasirin, Nunuk Dyah Retno Lastuti, Mochammad Amin Alamsjah, Win Darmanto, Sapto Andriyono
      Pages: 298 - 305
      Abstract: Rasbora (Rasbora sp.) is one of the endemic freshwater fish species in East Java, which is currently being threatened due to environmental degradation, human activities and pollution. In East Java, it is known that there are several species of Rasbora (Rasbora sp.). Domestication is one method to preserve Rasbora in East Java, preceded by identifying potential species of Rasbora sp. from the natural catchment. This study aims to determine the diversity of Rasbora sp in East Java. Samples were taken at five different locations, specifically: Nganjuk (Brantas Watersheds), Ngawi (Bengawan Solo Watersheds, Silowo Springs Tuban, Umbulan Springs Pasuruan and the Setail Watersheds Banyuwangi. The main parameters observed were morphology and morphometry. The morphological analysis used identification guidelines, while morphometric data were obtained through 15 parameters measurement then analyzed using one-way Anova test on SPSS 25 software. Based on the morphological and morphometric analysis, it is known that the species of Rasbora in Nganjuk and Ngawi regencies are Rasbora argyrotaenia and the species of Rasbora in Tuban, Pasuruan and Banyuwangi regencies are Rasbora lateristriata. One-way Anova test showed Rasbora from the five locations were significantly different based on the result value of p<0.05.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.30956
      Issue No: Vol. 11, No. 3 (2022)
  • Combination of Nigella sativa and Phyllanthus niruri as An Immunostimulant
           for The Prevention of White Spot Disease in Litopenaeus vannamei

    • Authors: Wida Lesmanawati, Wasmen Manalu, Min Rahminiwati, Muhammad Agus Suprayudi, Sri Nuryati
      Pages: 306 - 316
      Abstract: Pacific whiteleg shrimp (Litopenaeus vannamei) is a very important commodity, which accounts for almost 70% of the total world shrimp production. However, this production is still threatened by disease attacks, one of which is caused by white spot disease (WSD). Herbs are widely used as immunostimulants as an effort to prevent diseases. Black cumin (Nigella sativa) and stone breaker (Phyllanthus niruri) have long been known as excellent immunostimulants in human. This study aimed to evaluate the potential of N. sativa and its combination with P. niruri as an immunostimulant in L. vannamei for preventing WSD. Tested shrimp (±3.0 g) were treated by N. sativa (N), P. niruri (P) and the combination of both (1N:1P, 2/3N:1/3P, 3/4N:1/4P, 1/3N:2/3, 3/5N:2/5P, 1/4N: 3/4P, 2/5N:3/5P) through feed for 28 days. They were then infected by white spot syndrome virus (WSSV) and observed for 7 days. The parameters observed included immune response and production performance. N. sativa was able to reduce the mortality of test shrimp infected with WSSV, with an RPS value of 71%. N. sativa can be combined with P. niruri in the right composition (1/2N:1/2P and 2/3N:1/3P), while the other combinations are antagonistic. The administration of the best treatments in this study, relatively did not affect the value of total hemocyte count, phenoloxidase activity, respiratory burst activity, hemolymph clotting time and production performance of tested shrimp.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.27470
      Issue No: Vol. 11, No. 3 (2022)
  • Analysis of Nutritional Value and Consumer Behavior Diversification of
           Milkfish (Chanos sp.) Products From Aquaculture Products By Packaging
           Modification In MSME Sukolilo, Surabaya

    • Authors: Patmawati Patmawati, Sri Subekti, Wahju Tjahjaningsih, Ida Ayu Puspitasari, Annisa Nur R. P., Mohamad Akmal A.H., Renyta Andini; W., Aliffiansyah Rizky E
      Pages: 317 - 326
      Abstract: Analisis nilai gizi dan prilaku konsumen merupakan aspek penting untuk peningkatan nilai jual dari produk baru. Perbaikan pada kemasan dan informasi nilai gizi adalah salah satu cara untuk meningkatkan branding sebuah produk dan meningkatkan nilai jual produk terutama di masa pandemic Covid-19. Oleh sebab itu, peneltian ini bertujuan untuk menganalisis nilai gizi dan perilaku konsumen pada produk otak-otak dan bandeng presto yang diolah oleh salah satu UMKM di Surabaya. Metode yang digunakan yaitu dengan analysis nilai gizi produk yang diolah oleh UMKM dlabolatorium dan analisis prilaku konsumen dengan menyebar kuisioner ke 50 responden dengan berbagai latar belakang Pendidikan. Hasil uji analisis nilai gizi kedua produk untuk bandeng presto yaitu kadar air sebesar 61%, kadar abu 7.64%, kadar lemak 6%, dan kadar protein 8.79%. Sedangkan untuk otak-otak bandeng mengandung kadar air 58.20%, kadar abu 3.12%, kadar lemak 13.42%, dan kadar protein 7.69%. Hasil analisis prilaku konsumen menunjukkan bahwa konsumen memilih produk yang menggunakan kemasan yang menarik yang dilengkapi kemasan sekunder dan primer dengan teknologi vakum dibandingkan dengan kemasan mika untuk produk bandeng presto atau daun pisang untuk produk otak. Pengaruh penggunaan kemasan juga tentunya akan bepengaruh terhadap nilai tambah dan umur simpan produk. Hasil riset menunjukan juga bahwa konsumen lebih memilih belanja online sebanyak 94% responden dengan mempertimbangan beberapa informasi seperti nilai gizi yang disajikan dalam kemasan. Oleh sebab itu, penjualan produk diversifikasi perikanan memupnya potensi pemasaran yang lebih luas menggunakan platform online dibanding penjualan langsung dipasar. Hal ini juga akan berpengaruh terhadap omzet dan peningkatan perekonomian UMKM jika terus didampingi dalam pengembangan penjualan secara digital.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.31382
      Issue No: Vol. 11, No. 3 (2022)
  • Embryonic and Larval Development Stages of the African Catfish Clarias
           gariepinus (Burchell, 1822) (Teleostei, Clariidae) in The Ouargla, Algeria

    • Authors: Ibrahim elkhalil BEHMENE, Benabdallah Bachir Bouiadjra, Miloud Attou, Abdelkader Homrani
      Pages: 327 - 334
      Abstract: This study was designed to investigate for the first time in the region of Ouargla, Algeria, the events and timing of the embryonic development of African catfish, C. gariepinus (Burchell, 1822). The embryonic development was carefully monitored using a binocular magnifier 10 and 20 lenses that reveal details on live specimens from fertilization to the first take of food. The offensive and foul odor continues to be the characteristic smell of the hatching stage. Pigmentation and continuous spread of cephalo-caudal melanophore in fry hatched on the first day. The barely hatched larvae are photo-phobic. The yellow reserve is reduced and leads to the ability to swim easily, and the majority starts feeding on the fourth day. The vitellin reserve is significantly reduced, allowing the larvae to feed exogenously. The events observed in our opinion demonstrate the presence of two exceptionally critical parameters: the use of a high-quality diet and the guarantee of a clean and quality source of water, meeting the standards of aquaculture farming.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.31871
      Issue No: Vol. 11, No. 3 (2022)
  • Effect of Additional Aloe Vera (Aloe vera) on Artificial Feeds to Blood
           Cell Profile and Growth of Milkfish Seed (Chanos chanos)

    • Authors: Linayati Linayati, Nur Tri Jayanto, Tri Yusufi Mardiana, Muhammad Zulkham Yahya
      Pages: 335 - 344
      Abstract: Problems in cultivating milkfish are disease outbreaks and the high cost of feed. Therefore, a breakthrough is needed to overcome this problem by adding medicinal plants, namely aloe vera, to the feed. This study aims at determining the effect of adding aloe vera to artificial feed on the blood cell profile and growth of milkfish seed. The research method used was a complete randomized design with 4 (four) treatments and 3 (three) replications. The treatments on the test feed used were: A, (giving aloe vera as much as 0 g/kg feed), B (giving aloe vera as much as 5 g/kg feed), C (giving aloe vera as much as 10 g/kg feed), and D (giving aloe vera as much as 15 g/kg feed). The results showed that blood glucose values were still within the normal range. The lowest value in treatment was A, which was 42 mg/dL, and the highest in treatment was D, which was 66 mg/dL. The lowest Hb value was found in treatment A (5.1 g/dL), and the highest was 7.2 g/dL, found in treatment D. The highest average growth rate was obtained in treatment D with increasing biomass of 3.81g. While the lowest growth was obtained in treatment A which resulted in average growth of 2.06g. Water quality during the study was suitable for milkfish rearing media, namely temperature of 27 - 31°C, pH of 7.2 -,7,8, and salinity of 25 - 27 ppt.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.32688
      Issue No: Vol. 11, No. 3 (2022)
  • Vertical Infections and Prevalence of Viral Nervous Necrosis (VNN) in
           Milkfish (Chanos chanos)

    • Authors: Sang Ayu Made Putri Suryani, Ni Made Darmadi, Made Goura Premananda, Gusti Ngurah Permana
      Pages: 345 - 351
      Abstract: Milkfish juvenile is the leading fishery species in Bali with an export value in 2019. The increase in demand is related to the intensification of aquaculture which causes various diseases, especially Viral Nervous Necrosis (VNN). VNN infection in juveniles has been through water media. The VNN infection in milkfish may occur vertically. This study aims to determine the vertical infection and prevalence of VNN in milkfish. The VNN examination was carried out using the reverse transcription real-time Polymerase Chain Reaction (rRT-PCR) method. This research was conducted at BKIPM Denpasar. Samples were milkfish eggs taken from 15 spawning ponds in Gerogak District, Buleleng Regency. The results showed 3 positive samples from the 15 samples tested. A4 VNN positive sample with Ct 39.29 showed a virus concentration of 146 copies, Sample F positive for VNN with Ct 38.79 showing the 1978 copies of virus concentration, Sample G was positive for VNN with Ct 39.16 showing a virus concentration of 1588. Positive results of VNN examination on milkfish eggs with real time PCR indicates the presence of vertical transmission of VNN. It is also supported by measurement of water quality in milkfish spawning ponds shows the parameters of temperature, DO and pH in optimal and appropriate conditions standard. The results of the observation of clinical symptoms also show the condition of milkfish in a healthy state seen from swimming behavior that does not show symptoms of VNN infection. there are 3 positive samples in sample A4 with a virus concentration of 1,466 copies, sample F with a virus concentration of 1,978 copies and sample G with a virus concentration of 1,588. Detected VNN in eggs milkfish showed vertical VNN infection. Prevalence of infection. Milkfish egg against VNN shows a value of 20% which is included in the frequent infection category.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.32798
      Issue No: Vol. 11, No. 3 (2022)
  • Characterization of Salt Quality from Artemia (Artemia franciscana)
           Cultured Water with Dunaliella salina as Natural Feed

    • Authors: Himna Sayyyidatul Islamiyah, Mochammad Amin Alamsjah, Laksmi Sulmartiwi
      Pages: 352 - 358
      Abstract: The high demand for domestic salt cannot be achieved by local salt production from traditional salt and PT. Garam fluctuates every year. In addition, the quality of traditional salt is still low. This causes the need for domestic salt consumption to be met by imported salt. Therefore, it is necessary to have an alternative to meet the domestic salt demand, namely the revitalization of salt ponds for the development of mass Artemia cultivation that produces both Artemia production and biomass and produces salt in one location. In its natural habitat, Artemia uses microalgae as its main food source. The quality of microalgae feeds greatly determines the production of Artemia franciscana. The availability of nutrients is a factor that determines the rate of growth, so the amount and quality of feed are the main factors to meet the nutritional content for optimal development. Therefore, the salt production in this study used saltwater cultured Artemia with Dunaliella salina as a natural feed with a water age of 3rd,7th, and 14th days. This study aimed to determine the best age of Artemia water cultured with Dunaliella salina as a natural feed in salt production. The results showed that the different ages in Artemia water cultured with Dunaliella salina had a significant effect (p < 0.05) on salt characteristics. The best result was D7 which is salt from Artemia cultured water with Dunaliella salina as a natural feed with water age 7th day. Their respective values are 0.66a + 0.33 water content, 91.55 + 0.32 NaCl, 0.87 water-insoluble part, 0.39% + 0.01 Ca, and 1.25a + 0.05 Mg.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.34790
      Issue No: Vol. 11, No. 3 (2022)
  • Determination of The Best Concentration of Chitosan as a Recirculation
           Filter for Growth and Survival of Tilapia (Oreochromis niloticus)

    • Authors: Miska Sanda Lembang, Widiawati Widiawati
      Pages: 359 - 366
      Abstract: Tilapia (Oreochromis niloticus) is a cultivated commodity that is widely developed because of its economic value and high nutritional content. However, in aquaculture activities, problems such as water pollution reduce water quality. This problem means continuous water changes to maintain water quality. A recirculation system can be a solution by reusing water. The use of filters is an important factor in a recirculation system. One alternative filter is chitosan derived from crab and shrimp shell waste. This study aims to determine the optimal concentration of chitosan as a recirculation filter. The study was conducted experimentally using a completely randomized design with four treatments and three replications. Each treatment is a variation of the concentration of chitosan as a filter. Analysis of measurements carried out was absolute weight growth, specific growth rate, survival, and water quality. Based on the ANOVA results, each treatment had a significant effect on the absolute weight growth of tilapia, P<0.05, with P3 being the most significantly different. At the same time, the specific growth rate and survival of tilapia did not significantly affect P>0.05. The water quality measurements showed that the temperature, pH, ammonia, and DO values ​​were still by the quality standards of tilapia aquaculture, with P3 treatment, which gave better water quality results. Based on the study results, the optimal concentration of chitosan as a filter in supporting tilapia growth was in the P3 treatment, which was 50 mg.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.34929
      Issue No: Vol. 11, No. 3 (2022)
  • Solid Fermentation of Pelletized Fish Feeds Containing Black Soldier Fly
           (Hermetia illucens) Larvae Meal to Enhance Growth Performance of Catfish
           (Clarias sp.)

    • Authors: Cita Mahardika Hariyono, Catur Sriherwanto, Harijono Harijono
      Pages: 367 - 379
      Abstract: Unsustainable use of fish meal as protein and lipid ingredients in aquafeed has driven the search for alternative. Black soldier fly (BSF) (Hermetia illucens) larvae have been widely studied for the substitution purpose. This study aims at investigating the effect of solid fermentation of pelleted diets containing BSF larvae meal (BSFLM) on the growth performance of catfish Clarias sp. Four feed pellets were formulated: F1 (non-fermented, 60% BSFLM), F2 (fermented, 60% BSFLM), F3 (non-fermented, 50% BSFLM), and F4 (fermented, 50% BSFLM). These formulated diets and commercial feed pellets (positive control) were separately fed to the catfishes in 5 aquaria (10 fishes per aquaria in triplicate). Results showed that the fermentation increased the protein level by 10–11%, but kept the crude lipid concentrations unchanged. Specific growth rate (SGR) and weight gain (WG) profiles for fermented feeds F2 and F4 were higher than the non-fermented feeds F1 and F3, respectively. F4 gave the best feed conversion ratio (FCR) of 1.78, which was 15–25% more efficient than F1, F2, and F3. Compared to the commercial feed, the 4 formulated feeds underperformed by around 50% in all of the growth parameters except survival rates (SR) which were not significantly different (93–100%).
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.34956
      Issue No: Vol. 11, No. 3 (2022)
  • Vitamin C Improves the Efficacy of Oxytetracycline in Treatment of
           Aeromonas hydrophila-infected Juvenile Catfish (Clarias gariepinus)

    • Authors: Afisu Basiru, Abdulateef Abiodun Ajadi, Olayiwola Akeem Ahmed, Kolawole Yusuf Suleiman, Saliu Akanni Ameen
      Pages: 380 - 390
      Abstract: The recent global increase in demand for catfish products has led to intensive catfish farming, favoring Aeromonas infections. Therefore, there is a need to enhance the current management method of Aeromonas infection in catfish. This study evaluated the efficacy of adding vitamin C to Oxytetracycline in the treatment of Aeromonas infection through the development of skin lesions, mortality and serum antioxidant defense system. One hundred and five juvenile catfish were randomly assigned to seven groups (n=15). The first group served as control and was neither infected nor treated, while the other groups were infected with Aeromonas hydrophila. Seventy-two hours after inoculation, catfish in groups 2-7 were treated as follows: no treatment, Oxytetracycline through bath, Oxytetracycline through feed, Oxytetracycline through bath with 500 mg of vitamin C in water, Oxytetracycline through feed with 500 mg of vitamin C in water and 500 mg of vitamin C only in water, respectively. The treatment was done for five consecutive days. Behavioral changes, clinical signs and mortality were observed. Data were presented as Mean±SD and analyzed using descriptive statistics or One-way Analysis of Variance (ANOVA) and Tukey's Test. p <0.5 were regarded as significant. The cumulative percentage mortality in groups (Groups 5 and 6) supplemented with Vitamin C was 26.7 and 33.3 compared to 90% and 46.6% in groups 2 and 4, respectively. The results showed that adding vitamin C to Oxytetracycline reduced the development of skin lesions and mortality. Therefore, Vitamin C is recommended to manage Aeromonas hydrophila infection in catfish.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.35153
      Issue No: Vol. 11, No. 3 (2022)
  • Detection of Salmonella spp., Escherichia coli, and Listeria monocytogenes
           in Tuna by Multiplex PCR

    • Authors: Kristina Natalia, Mariana Wahjudi, Tjie Kok
      Pages: 391 - 401
      Abstract: Fishery products are types of commodity with various levels of development including the addition of food additives. They are very susceptible to patogenic microbial contamination hence they can cause food poisoning and cause disease outbreaks in consumers. In this study, tuna fish spiked with Salmonella spp., Escherichia coli, and Listeria monocytogenes bacteria is examined for the bacteria contamination. The examination of these patogenic bacteria is carried out using multiplex PCR (mPCR), a modern technique developed to detect target DNAs of bacteria simultaneously. The results of the examination are compared to biochemical test for sensitivity and time-efficiency. Based on the results obtained, mPCR method is ±1250-fold more sensitive and ±80-fold more time-efficient than the biochemical tests. Therefore, mPCR method could be recommended to be used as a tool for detection of bacteria in fishery products.
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.36509
      Issue No: Vol. 11, No. 3 (2022)
  • Correlation of Density and Water Quality to Cortisol of Cantang Grouper
           Fish (Epinephelus fuscoguttatus X Epinephelus lanceolatus) in Floating Net

    • Authors: Laksmi Sulmartiwi, Bintang Eka Nugraha Satia Aditama, Isnatul Umu Shohifah, Alfian Rahmadhani Satria Hani Saputra
      Pages: 402 - 411
      Abstract: Cantang grouper is a hybrid resulting from crosses between female tiger grouper (E. fuscoguttatus) and male kertang grouper (E. lanceolatus). Cultivation with floating net cages is one of the attempts made to cultivate cantang grouper fish. Problems that arise are the non-uniformity of the size of the fish when harvesting, this problem can lead to a decrease in selling prices and maintenance that tends to be longer. Metabolic disorders in living beings can be caused due to physiological stress and one of the causes can arise from water quality. Water quality is closely related to feeding and stocking density because the increase in feed and stocking density can affect the accumulation of feces to stress which causes slow growth. This study was conducted to find out the influence of density and water quality factors that play the most important role in the stress of cantang grouper fish based on indicators of cortisol levels. Cortisol testing is performed using the elisa method with Bioassay Technology Laboratory E0014Fi kit type. Data are processed using multiple linear regression test analysis that serves to determine the relationship and influence of the free variable (independent) X on non-free variables (dependent) Y. Regression analysis is used to determine the relationship between factors that are causal relationships. After the regression test, then the Analysis of Variance (ANNOVA) test is conducted, to find out the difference and influence of dependent parameters with significantly independent. There is a strong influence and correlation between water quality factors simultaneously to the cortisol levels of cantang grouper fish in floating nets, as can be seen from the R value of 0.546. The most influential water quality factor was nitrite with an effect of 19.7%. The next most significant water quality factor was ammonia with an effect of 37.1%. The indicated cortisol value can be known that fish are not in a state of stress due to increased NO2 and NH3. Density has no significant effect on cortisol because of its significance value above (p>0.05).
      PubDate: 2022-09-02
      DOI: 10.20473/jafh.v11i3.36661
      Issue No: Vol. 11, No. 3 (2022)
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