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- STUDY OF WATER PHYSICS, CHEMISTRY, AND BIOLOGY QUALITY IN SWAMP WATER HIGH
TIDE CONDITIONS IN BANYUASIN REGENCY, SOUTH SUMATERA
Authors: Raudhatus Sa'adah, Rizki Eka Puteri, Selly Ratna Sari
Pages: 1093 - 1100
Abstract: This study was conducted in tidal lowland at Banyu Urip Village, Tanjung Lago District, Banyuasin Region, South Sumatera. Samples were collected two times. The water sample was collected during high tide in primary, secondary, and tertiary canals. The result showed the seasonal variation of physical and chemical water parameters such as temperature 27 – 30 ºC, pH 3 – 5, DO 3.7 – 4.5 mg/l, phosphate 0.01 – 0.02 mg/l, TSS 108 – 232 mg/l, and EC 11 – 107 µmhos/cm. This study also identified 10 classes and 10 genera of phytoplankton. It has potential as a natural food source. The result revealed that physical and chemical water quality parameters were still suitable for aquaculture based on Government regulation of Republic Indonesia number 82 year 2001 about water criteria class III for fisheries. Meanwhile, the great challenge of tidal lowland for fishery management is low pH. Therefore, pre-treatment to increase pH before fish rearing is required.
PubDate: 2021-02-15
DOI: 10.23960/.v9i2.p1093-1100
Issue No: Vol. 9, No. 2 (2021)
- STUDY OF PITUITARY GLAND EXTRACT UTILIZATION FROM STRIPED CATFISH WASTE
FOR REPRODUCTION PERFORMANCE IMPROVEMENT OF NORTH AFRICAN CATFISH (Clarias
gariepinus)
Authors: Yeni Elisdiana, Dio Vinski Aquardo, Munti Sarida, Siti Hudaidah, Oktora Susanti, Maulid Wahid Yusup
Pages: 1109 - 1116
Abstract: One of the problems in North African Catfish (Clarias gariepinus) breeding is the inability to spawn spontaneously due to a specific spawning season. This happens due to reproductive dysfunction resulting in a slow development of gonads in catfish broodstock. This research aimed to study the effect of injection of pituitary gland extract from Striped Catfish head waste on the spawning performance of North Africans Catfish. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments were A (pituitary gland 0 mg/kg broodstock), B (pituitary gland 200 mg/kg broodstock), C (pituitary gland 300 mg/kg broodstock), and D (pituitary gland 400 mg/kg broodstock). The spawning process was performed with a ratio of 1:1 between male and female broodstock. The pituitary gland was injected into the head of female catfish at 07:30 pm and at 04:00 am. The research parameters observed were relative fecundity, fertilization rate (FR), hatching rate (HR), and survival rate (SR). The results showed that injection of the pituitary gland from Striped Catfish head waste could increase spawning performance with relative fecundity reaching of 31,357±12,265 eggs/ kg of female broodstock, FR 65±4%, HR 74±14%, and SR 81±10%. Therefore, hypophysation by utilizing Striped Catfish head waste is effectively increases the spawning performance of North African Catfish (Clarias gariepinus) seen from the high values of relative fecundity, FR, HR, and SR of larvae in treatment B (200 mg/kg broodstock).
PubDate: 2021-02-20
DOI: 10.23960/.v9i2.p1109-1116
Issue No: Vol. 9, No. 2 (2021)
- GROWTH PERFORMANCE AND IMMUNE RESPONSE OF SILVER POMPANO SEEDS
Authors: Herno Minjoyo, Arief Prihaningrum, Arief Rahman Rivaie, Verly Dharmawanti
Pages: 1117 - 1130
Abstract: The increase of the growth performance and control of disease can be done by the use of imunostimulan. Immunostimulant is a material that capable of increasing the non-specific immune response of fish. The purpose of this study was to determine the best concentration of immunostimulants in self-formulated pellet feed on the growth performance and immune response of silver pompano fingerling. The immunostimulant content used it was sourced from commercial products Nutricell. There was three concentrations of imunostimulan were used in this study, namely 0.3% (treatment A), 0.5% (treatment B), and 0,0% (treatment K) and one group fish fed with commercial feed as the control and a comparison (treatment P). A total of 840 silver pompano fish seeds with an average initial body weight of 11,13± 1,39 g were kept in 12 cages (0.8 x 0.8 x 0.6 m3) for 30 days. The results showed that the provision of feed supplements containing commercial immunostimulants did not significantly affect the growth rate, The feed conversion and survival rate but produced the highest total leukocyte count of 65,775. x 10. cells/ml and 35.10% phagocytosis rate achieved on the 30th day of treatment. The results of the five-day challenge test showed that treatment A which was the addition of 0.3% immunostimulant feed had lower mortality than other treatments, after being injected with Vibrio alginolyticus bacteria at a dose of 3 x 109 cfu/ml/0.002 g/head fish weight. Based on the results above, it can be concluded that the formulated feed in treatment A which was added with immunostimulant 0.3% (0.7% total in the feed formula) showed a higher survival performance in the nursery phase of silver pompano fish compared to other treatments.
PubDate: 2021-02-21
DOI: 10.23960/jrtbp.v9i2.p1117-1130
Issue No: Vol. 9, No. 2 (2021)
- MOLECULAR IDENTIFICATION AND LOCAL ISOLATE BACTERIAL ACTIVITY TEST AS
BIOCONTROL CANDIDATES TO TACKLE Vibrio Spp INFECTIONS AT VANNAMEI SHRIMP
CULTIVATION (Litopenaeus vannamei) IN EAST LAMPUNG
Authors: Heidy Riana, Supono Supono, Agus Setyawan
Pages: 1131 - 1142
Abstract: Pacific white shrimp (Litopenaeus vannamei) is a widely cultivated type of shrimp with high economic value. One of the obstacles in pacific white shrimp cultivation is an infectious disease, especially Vibrio spp. This study aims to identify bacteria of biocontrol candidate against Vibrio spp. This research was conducted by the Laboratory of Fisheries and Marine, Faculty of Agriculture, University of Lampung and the laboratory of PT. Indolab Utama, West Jakarta, from September 2019 to January 2020. Bacteria of Biocontrol candidate were isolated from traditional shrimp pond water in Purworejo Village, Pasir Sakti District, East Lampung Regency. Water samples were taken from the inlet, plot, and outlet. The bacterial screening was carried out by testing the antagonism of bacterial isolates isolated from water samples with several Vibrio bacteria, namely V. harveyi, V. algynoliticus, and V. parahemolyticus on Sea Water Complete (SWC) medium. The bacterial isolates with the highest inhibitory activity were then identified morphologically, biochemically, and molecularly by 16s rRNA. The results showed that from those 10 isolates was found 1 bacterial isolate from water samples. Only one pond plot isolate (P1) was found that had inhibitory activity against V. harveyi and the size was 21 mm. The morphological test results showed the colonies were round, flat edges, convex elevation, cream colored, and included in the negative gram bacteria group. The results of biochemical tests showed that the bacteria were motile. Meanwhile, the molecular test results with 16s rRNA showed that the P1 bacteria were 99% similar to Aeromonas caviae isolates, which were found potential as biocontrol candidates because they can inhibit the growth of V. harveyi bacteria, which was characterized by the formation of a clear zone around the paper disk.
PubDate: 2021-02-27
DOI: 10.23960/jrtbp.v9i2.p1131-1142
Issue No: Vol. 9, No. 2 (2021)
- QUALITY IMPROVEMENT OF FEED CHEMICAL COMPOSITION WITH THE ADDITION OF
DRAGON FRUIT SKIN FLOUR (Hylocereus polyrhizus)
Authors: Tulas Aprilia, Rakhmawati Rakhmawati
Pages: 1101 - 1108
Abstract: Providing high-quality feed is one of the efforts to increase fish productivity and growth and reduce fish mortality. One of the additional alternative feed ingredients as a source of vegetable protein is dragon fruit peel which can be used as an additional ingredient in fish feed. The study of the chemical composition of feed with the addition of dragon fruit peel flour is essential information to determine the effect of addition and the benefits of by-products (waste) from food processing in aquaculture development. This study aims to evaluate the quality of the chemical composition of feed after being treated with the addition of dragon peel flour through proximate analysis. The test was carried out at the Agricultural Product Technology Laboratory of Lampung State Polytechnic, from June to August 2020. The test results showed that dragon fruit peel flour had a carbohydrate content of 50.74%, crude fiber 26.22%, ash 21.35%, protein 5.08%, and fat 4.80% (dry weight). The test results showed that the addition of dragon fruit peel flour increased the protein content in the feed, which increased with the increase in the added dragon fruit flour. The highest protein content in treatment was 1.5%, namely 37.79% (dry weight), and decreased in the addition of dragon fruit flour by 2.0%. The highest ash content in the addition of 2.0% was 11.32% (dry weight). The content of crude fiber, carbohydrates and fat has decreased with the increasing dose of dragon fruit flour. The addition of dragon fruit peel flour can increase the protein content in the feed to substitute in the manufacture of fish feed. This also increases the utilization of dragon fruit peel waste, especially in aquaculture, to reduce waste disposal into the environment.
DOI: 10.23960/.v9i2.p1101-1108
Issue No: Vol. 9, No. 2
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