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Savana Cendana
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Online) 2477-7927
Published by Universitas Timor Homepage  [2 journals]
  • The Activity of Tannin Acyl Hydrolase Enzyme at Optimum Condition of
           Lactobacillus Plantarum Fermentation using Canna Flour (Canna edulis Kerr)
           as Substrate

    • Authors: Azor Yulianus Tefa, Marselina Theresia Djue Tea
      Pages: 59 - 60
      Abstract: This study aimed to determine the activity of tannin acyl hydrolase enzyme produced under the optimum conditions of Lactobacillus plantarum fermentation using canna flour (Canna edulis Kerr) flour as substrate. The research process began with the production of tannin acyl hydrolase enzyme, then proceeded with the isolation of the crude enzyme and then the activity of the tannin acyl hydrolase enzyme were measured. The variations of inoculum volume in this study were 1 mL, 3 mL, and 5 mL, with 2 grams of substrate. The variation of pH used when testing the enzyme activity was pH 4, pH 5, pH 6, and pH 7, the temperature variations used were 35°C, 40°C, 45°C, and 50°C, while the incubation time variations were 12 hours, 24 hours, 36 hours, and 48 hours. The results showed that the highest activity of tannin acyl hydrolase enzyme was obtained at 1.13 U/mL at optimum pH of 6, 0.88 U/mL at optimum temperature of 45°C, and 0.89 U/mL for optimum incubation time of 36 hours.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1932
      Issue No: Vol. 7, No. 04 (2022)
  • Prediction of Seed Deterioration Using Fisiology and Biochemical Tests

    • Authors: Gani Jawak, Eny Widajati, Devi Liana, Tri Astuti
      Pages: 61 - 64
      Abstract: The seed viability test is one of the routine tests carried out in the seed certification process. Decreasing the viability of seeds can be done biochemically and physiologically. This research aimed to study of seeds deterioration through physiological and biochemical tests. This study used two lots of long bean seeds (high and low lot). Physiologically, the seed viability test was carried out by means of germination test and biochemical by means of respiration test using tetrazolium (TTZ) staining technique and titration method. The experiment designed by completely randomized design one factor. Data were analyzed by anova test and Pearson correlation. The results of research showed that the seed lot treatment was significantly on the tetrazolium test and the total dry weight of sprouts, while the vigor index, seed germination, and respiration index measurements did not show significant. There was a high positive correlation in the high respiration rate of high seed lots and low seed lot seeds on total dry weight of germination, germination, vigor index, and tetrazolium test. Therefore, biochemical tests with TTZ and respiration with titration methods can be used as alternatives to predict rapid deterioration of seeds.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1921
      Issue No: Vol. 7, No. 04 (2022)
  • Eggshell and Rice Washing Water as Nutrition DFT (Deep Flow Technique)
           Hydroponic on The Growth of Red Spinach and Green Spinach

    • Authors: Yuda Arif Yuniansyah, Ahmad Arif Darmawan, Slamet Rohadi Suparto, Agus Suyanto
      Pages: 65 - 68
      Abstract: The purpose of this research is to know the effect of adding the egg shell and washing rice water to the solution of nutrients (AB-mix ) on the growth and results of red and green spinach, to know the growth of red spinach and green spinach and to know the interaction between egg shell and rice washing water addition in to AB Mix solution on the growth and results of red and green spinach. The method used in this research was Complete Randomized Block Design (RAKL) with two factors with 3 replications. Factor first was A0 treatment (AB Mix 1%), A1 (AB Mix 1% and egg shell 1%), A2 (AB Mix 1% and rice washing water 1%), A3 (AB Mix 1%, egg shell 0.5% and rice washing water 0.5%) and factor the second was are red spinach and green spinach varieties. The parameters measured were plant height, leaf number, leaf area, root length, fresh crown weight, dry crown weight, fresh root weight, dry root weight. The results showed that A2 (AB Mix 1% and rice washing water 1%) could increase plant height, leaf number, leaf area, root length,fresh crown weight, fresh root weight, dry crown weight and dry root weight in red spinach and green spinach. Green spinach growth was better than the red spinach. The interaction between egg shell and rice washing water addition in to AB Mix solution with the type of spinach took effect in the number of leaves, plant height and dry root weight.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1883
      Issue No: Vol. 7, No. 04 (2022)
  • Growth and Yield of Soyabean (Glycine max (L.) Merr.) With Fertilization
           Of Goat Cage And Bio-Growth

    • Authors: Jakoni Jakoni, Wisnu Giri Madani, Tiara Septirosya
      Pages: 69 - 72
      Abstract: Soybean is one of the third most essential crops after rice and maize, whose production still needs to be increased. One of the efforts to utilize less fertile land is using organic fertilizers in goat manure and Bio-Growth. This study aimed to obtain the best dose of goat manure and Bio-Growth for soybean growth and yield. This research has been carried out at the experimental garden of the Kubang Research and Assessment of Agricultural Technology (BPTP) Balitbangtan Riau from August to November 2021. The method is practical with a factorial Randomized Block Design (RBD) consisting of 2 factorials with 3 replications. The first factor is goat manure (K0: 0 tons/ha, K1: 10 tons/ha, and K2: 20 tons/ha); the second factor is Bio-Growth (B0: 0 ml/4 l water, B1: 72.5 ml/4 l water, B2: 145 ml/4 l water, and B3: 217.5 ml/4 l water). The height of the plant, the number of its leaves, the diameter of its stem, the number of its branches, the number of pods produced by each plant, the weight of pods produced by each plant, the total weight of pods, and the weight of one hundred seeds were all measured. The application of goat manure and Bio-Growth influenced the height of the plant, the number of leaves, the number of pods produced by each plant, the weight of each pod, the total weight of the pods, and the weight of one hundred seeds. It was determined that the interaction between the two treatments did not impact all experimental parameters. This study concludes that the best application of goat manure is 10 tons/ha, while the most efficient Bio-Growth dose of 72.5 ml/4 l of water can increase the growth and yield of organic soybeans.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1898
      Issue No: Vol. 7, No. 04 (2022)
  • The Effectiveness of Natural Air Drying Time on The Quality of Ant Nests
           (Myrmecodia tuberosa) Using A Batch Dryer

    • Authors: Junita Magdalena Seuk, Jefrianus Nino, Maria Angelina Tuas
      Pages: 73 - 76
      Abstract: Ant nest (Myrmecodia tuberosa) is one of the plants that which contains flavonoids, tannins and polyphenols which functions as an antioxidant. One of the post-harvest handling technologies in maintaining the quality of the material that is drying. In the area of Timor natural air very potential in the drying process, because it is environmentally friendly and free. This study aims to determine the effectiveness of time natural air drying on the quality of ant nests using batch dryer drying and knowing the content of phytochemical compounds (flavonoids and tannins). This research is an experimental research designed using a single factor Completely Randomized Design with time variable drying (A) which consists of 3 levels, namely the drying time of 5 hours (A1), 10 hours (A2) and 15 hours (A3) and repeated 2 times so that there are 6 units treatment. Drying is carried out with a thickness of 2 mm. slices of ant nests mm. Determination of flavonoid content and tannin content was determined by the method quantitative. Based on the results of the study, 10 hours of drying produced levels of The highest flavonoid was 45.59 mg/g and the tannin content was 94.29 mg/g with the final water content is 26.04% bw. The content of phytochemicals (flavonoids and tannins) in ant nests, namely before drying with flavonoid content of 42.59 mg/g, 5 drying hours with 33.59 mg/g flavonoid content, 10 hours drying with flavonoid content of 45.06 mg/g and at 15 hours of drying with a concentration of flavonoids 20.42 mg/g.The tannin content before drying was 106.88 mg/g, 5 hours of drying with a tannin content of 74.55 mg/g, 10 hours of drying with tannin content was 94.29 mg/g, and at 15 hours of drying it was 92.62 mg/g.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1576
      Issue No: Vol. 7, No. 04 (2022)
  • The Testing of Several Water Media on Young Shoots and Roots Growth of
           Lesser Yam (Dioscorea esculenta L.) Tubers from Serui Papua

    • Authors: Siska Syaranamual, Yaved Muyan, Inna Rumainum, Mei Vora Manori
      Pages: 77 - 80
      Abstract: The richness of genetic resources in Papua Province, with its various potentials, has not been studied and utilized in a sustainable manner in order to reduce and prevent germplasm genetic erosion. Thus, optimal management of germplasm is an inseparable part of efforts to maintain genetic resources in order to sustain genetic resources. One of them is the lesser yam which has high cultural value because it is used in traditional ceremonies and is also used as a wedding dowry and as a complement in traditional ceremonies. This means that the cultivation of lesser yam has been integrated into the life of the Papuan people. This study aims to examine several water media on the growth response of young shoots and roots of gembili plants as a cultivation technique to shorten the dormancy period and produce planting material or gembili seeds from Serui Papua. The study was conducted for two months utilizing the Completely Randomized Design (CRD) method which consisted of two treatments, namely immersion in sterile water/aqua/water and well water, the treatment was repeated three times, thus, six experimental units were obtained. From the observations, it can be concluded that using the cultivation technique of soaking tubers with sterile water or aqua water can shorten the dormancy period and increase planting material or gembili (Dioscorea esculenta L.) seeds from Serui Papua. The results of data analysis prove that the application of water media has a significant effect on the growth of shoots and roots on gembili tubers. The comparison of aqua water treatment and well water showed consistency where aqua water treatment expressed the average number of shoots and number of roots at 4 WAP, more than the average of the two parameters in the well water treatment at 5 WAP. It is recommended for further research using the same treatment and other alternative water growing media by extending the observation time.
      PubDate: 2022-10-31
      DOI: 10.32938/sc.v7i04.1675
      Issue No: Vol. 7, No. 04 (2022)
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