|
|
- Detection of Mycoplasma equigenitalium from Genital Tract of Healthy
Domestic Donkeys (Equus africanus asinus)
Authors: Pablo Tamiozzo, Erika Sticotti, Ana Flores Bragulat, Carolina Alonso, Mauro Mació, Luis Losinno
Pages: 33–3 - 33–3
Abstract: Mycoplasma equigenitalium (M. equigenitalium) has been identified in the reproductive tracts of both fertile and infertile mares and stallions. However, there are scarce antecedents on its detection in the reproductive tract of donkeys. A cross-sectional study was conducted with healthy domestic donkeys. Jennies (n=6) and jacks (n=6) were sampled through the collection of vaginal and preputial swabs. The specimens were plated into a commercial Mycoplasma medium and analyzed through the use of a species-specific PCR. M. equigenitalium was detected by PCR in four specimens (4/12), one from a jack (1/6) and three from jennies (3/6). Moreover, from one of the PCR positive jennies, M. equigenitalium could be isolated. Presumably, this is the first report of M. equigenitalium detection from healthy domestic donkeys by both culture and PCR methods despite the small number of analyzed specimens. The obtained results are a starting point for the study of Mycoplasmas affecting donkeys.
PubDate: 2022-09-15
Issue No: Vol. 1, No. 2 (2022)
- Isolation, Culture, Identification, and Bioenergetics Metabolism of Equine
Skeletal Muscle Satellite Cells
Authors: Xinzhuang Zhang, Gerelchimeg Bou, Jingya Xing, Yali Zhao, Caiwendaolima, Manglai Dugarjaviin
Pages: 36–4 - 36–4
Abstract: Equine skeletal muscle satellite cells (EMSCs) are muscle stem cells in horses, responsible for the postnatal growth, repair, and homeostasis of skeletal muscles. EMSCs are an attractive model for horses to investigate the mechanisms of muscle growth and spontaneously fuse to form differentiated muscle fiber types through activating a battery of muscle-specific genes. Previous reports on the successful isolation and culture of skeletal muscle satellite cells mostly used skeletal muscles of young animals. With the high value of horses, skeletal muscle samples of foals are very difficult to obtain. The present study describes protocols for enriching the satellite cell fraction from the semitendinosus of a 2-year-old Mongolian horse to isolate the EMSCs. Optimized culture conditions with gelatin layering accelerated the adhesion speed of EMSCs. The identification of EMSCs was carried out through multiple dimensions including cell morphology, myogenic induction, differential adhering, and molecular signatures. In particular, the Seahorse Extracellular Flux analyzer was utilized for evaluating the bioenergetics metabolism of EMSCs by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). The present study provides reference for the isolation, purification, identification, and bioenergetics metabolism characteristics of EMSCs, which would be useful for studying the molecular mechanisms for muscle development, muscle fiber type differentiation, and recovery from muscle injury in horses.
PubDate: 2022-09-15
Issue No: Vol. 1, No. 2 (2022)
- Fitness of Eventing Horses Submitted to Interval Training
Authors: Juliana Azevedo Gonçalves, Nelson Alexandre Kretzmann Filho, Rafael Rodrigues, Willian Jadoski, Bruna Beretta, Ana Carolina Bahi Aymone , Adriana Pires Neves
Pages: 47–5 - 47–5
Abstract: The fitness of eventing horses was assessed in field tests through the use of heart and respiratory rates and blood biochemical parameters. The first stage (S1) lasted three days and consisted of the following three tests: the incremental speed test (IST), the incremental jumping test (IJT), and the jumping course (JC). Following the first stage, horses were submitted to a six-week interval training program and were then submitted to another evaluation, the second stage (S2), of the same tests as in the first stage. Lower heart rate (HR) values were observed during the incremental speed test in S1 than in S2. The enzymes creatine kinase (CK), aspartate transferase (AST), and lactate dehydrogenase (LDH) showed significant speed-related reductions in the second stage in comparison with the first stage. HR increased significantly during the incremental jumping test in the upper obstacle heights (55 and 70 cm). Respiratory rate showed a significant decrease in the last two laps and at 10 min after exercise in the second stage, compared to the first stage. Values of LDH were lower in the second stage than in the first stage, and AST values were significantly lower at 55 cm and 10 min after exercise in S2. During the jumping course, RR had better recovery in the second stage than in the first stage, and glucose decreased during the course in both stages. The enzymes CK and AST presented higher levels at 10 min after exercise in the second stage than in the first stage. The field tests can be utilized for evaluating the fitness of eventing horses.
PubDate: 2022-09-15
Issue No: Vol. 1, No. 2 (2022)
|
Open Access journal
