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- Fermented Purple Sweet Potato in Rations on Carcass Improvement,
Antioxidant Profile, Meat, and Eggs Lipid Profile of Bali Ducks
Authors: Tjokorda Gede Belawa Yadnya, Ida Bagus Sudana, I.G. A.I. Aryani
Abstract: This study aimed to determine the effort of fermented purple sweet potato in the rations to improve carcass, antioxidant profile, meat and eggs lipid profile of bali ducks. Completely randomized design with three treatments was used in this study. Sixty baliducks were divided into 3 treatments that provision by rations; A: without purple sweet potato, B: with 10% purple sweet potato, C: with 10% fermented purple sweet potato.The experiment used four replications to each treatment which 5 layer ducks were used as a repetition in each unit of experiment. The experiment was done for 12 weeks. Antioxidant consumption was counted during the experiment. In the end of experiment the ducks were killed and meat antioxidant profile namely antioxidant capacity, malondialdehida (MDA) and superoxide dismutase (SOD), carcass profile including slaughter weight, carcass weight, carcass percentage, and physical carcass composition (meat, bone, fat including skin) were recorded. Meat and egg lipid profile (total cholesterol, highdensity lipoprotein (HDL), low density lipoprotein (LDL) and triglycerides) were also counted in the end of experiment.The results showed fermented purple sweet potato in the rations significantly increased the consumption of antioxidants, accompanied by increasing in slaughter weight, carcass weight, carcass percentage, and meat percentage. Fermented purple sweet potato in the rations also significantly reduced the percentage of sub-cutaneous fat and skin. Fermented purple sweet potato improved antioxidant profile significantly, increased antioxidant capacity, and superoxide dismutase (SOD), and decreased the levels of malondialdehide (MDA). It also decreased significantly the total cholesterol, HDL, LDL and triglycerides in meat and eggs. The conclusion of the research is a 10 % fermented purple sweet potato in the rations be able to improve the antioxidant consumption, carcass, antioxidant profile, meat and eggs lipid profile of first laying phase of bali ducks.
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
- The Effect of Soyabean Extender on Viability and DNA Integrity of
Kintamani Dog Sperm on Cold Storage
Authors: I Made Yoga Windu Pradana, Wayan Bebas, I Ketut Puja
Abstract: The objective of this study was to evaluate the viability and integrity of canine semen diluted in egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. Semen was collected by manual manipulation from an apparently healthy kintamani dog stud. The semen was subjected to gross and microscopic examination to determine its viability following dilution in different extenders. Only semen with motility rate of 60% or higher was used in this study. Semen samples were diluted in Tris-citrate-glucose-soya bean and egg yolk glucose-citrate extenders at two levels of dilution; sperm to extenders ratio of 1:2 and 1:3. Following semen dilution, evaluations were made on sperm motility, percentage of live sperm, and DNA integrity at 0 hour, 3 hours, and 6 hours under 5oC of storage. Data was analyzed by multivariate analysis of variance (MANOVA).The present results showed a high significant different (p<0.01) in sperm motility, percentage of live and DNA integrity between treatment with egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. The result also showed that percentage of live sperm and DNA integrity of semen at 1:2 dilution difference significantly (p<0.01) to that of 1:3 dilution. The sperm viability and integrity were significantly difference (p<0,01) between the duration of storage at 5°C. Thus, the current study indicated that Tris-citrate-glucose- soya bean extenders was sufficient to maintain motility, viability, and DNA integrity of kintamani dog spermatozoa during storage at 5 oC. Further research should be conducted to evaluate the fertility of spermatozoa following dilution with these extenders.
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
- Coconut Water Based Extender Effects on Motility,Viability, and DNA
Integrity of Chilled Kintamani Dog Semen
Authors: I Wayan Nico Fajar Gunawan, I Made Kardena, I Ketut Suatha, I Ketut Puja
Abstract: This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
- A Survey on Cases of Canine Transmissible Venereal Tumor (TVT) in
Kintamani Dog
Authors: Claudia Murti Andari, I Made Kardena, I Ketut Puja
Abstract: The cases of canine transmissible venereal tumor (TVT) in Kintamani Bali Dog were studied by evaluating the cases in clinical records from some veterinary clinics in the two regencies of Badung, Gianyar and Denpasar City. A total of 486 clinical records on Kintamani Bali Dogs gathered from 2010 to 2014 was used in this study. Among those, the specific cases on TVT diagnostic were observed. The study showed that the prevalence of TVT was catagorised low. Three of Kintamani Bali Dogs were diagnosed to have TVT (0.62%). Canine transmissible venereal tumor affected both male and female dogs. The tumor cases were recorded in varied ages, from 2 to 5 years. This result indicates that TVT in Kintamani Bali Dogs is likely to be associated with the dog’s sexual maturity.
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
- The Effectivity of Ethanol Extract of Datura Metel L. Seeds as a General
Anaesthesia on Kintamani Dogs
Authors: I Putu Juli Sukariada, I Wayan Sudira, I Gusti Ngurah Sudisma
Abstract: This study aimed to determine the effectivity of ethanol extract of Datura metel L. seeds, as general anaesthesia on Kintamani dogs. A total of 25 dogs was divided into 5 groups, namely: group 1(treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 10 mg/kg BW), group 2 (treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 15 mg/kg BW), group 3 (treated by the combination of xylazin 1 mg/kg BW and ethanol extract of 10% Datura metel L. seeds 20 mg/kg BW), group 4 (treated by the combination of xylazin 1 mg/kg BW and ketamine 15 mg/kg BW), and group 5 (treated by the ethanol extract of 10% Datura metel L. seeds 15 mg/kg BW). Induction time, duration and recovery time were recorded, in which these responses were observed by clamping on the tail, ears and interdigital area. Physiological responses including respiration values, rectal temperature, oxygen saturation (SPO2), the frequency of the heart rate and electrocardiogram were observed using physiographic machine every 5 minutes until the dogs regain their consciousness. The level of ALT and AST was also examined to determine the level of toxicity of ethanol extracts of 10% Datura metel L. seeds. The results showed that group 1 and group 2 had no significant effect on the physiological response changes. However, the group 3 showed the sign of acute toxicity followed by increasing the levels of ALT and AST. The group 4 showed a decreasing physiological response, while group 5 had a similar response to group 2, but without sedation effect. It can be concluded that the ethanol extract of 10% Datura metel L. seeds has a potential as an analgesic and an anaesthetic agent.
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
- Effect of Maternal Antibodies on Histopathogenesis of Newcastle Disease
Virus in Broiler Chickens
Authors: I Made Galih Diparayoga, Nyoman Mantik Astawa, Anak Agung Ayu Mirah Adi
Abstract: The aims of this research were to overview the effect of maternal antibodies on the histopathological changes and viral antigen distribution of the broiler chickens challenged with ND APMV-1 virus. A total of 100 chicken were allotted into 3 treatment groups consisting of group I (titer antibodies< 23 HI Unit), group II (titer antibodies 23 – 24 HI Unit) and group III (titer antibodies> 24 HI Unit). All group I, II and III were inoculated with ND virus isolates of type viscerotropic velogenic at the dose of 1000 TCID50. The histopathological changes observed in nervous system were endotheliosis and perivascular cuffing. Immunohistochemistry staining showed that NDV infected cells were found in most organs both in inflammatory cells and in epithelial cell of many organs mainly in nervous, respiratory and digestive systems. Neurological symptoms and neural lesions were highest in group II (titer antibodies 23 – 24 HI Unit)
PubDate: 2016-02-01
Issue No: Vol. 4, No. 1 (2016)
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