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- Various Saccharides Addition of Extender Ram Efficiency to Improve
Cryopreservation in Semen Etawah Crossbreed Bucks with Seminal Plasma
Replacement
Authors: aeni nurlatifah, Pradita Iustitia Sitaresmi, Anita Hafid, Desiana Ade Mahari, Florentina Bety Indah Lupitasari, Rahma Isartina Anwar, Santoso Santoso, Muhammad Rizal, Herdis Herdis
Pages: 83 - 90
Abstract: Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise of the spermatozoa during liquid semen processing using milk or egg yolk substrates diluent. A plasma replacement process is performed to avoid this possibility. The cryopreservation process leads to sperm cell damage due to the freezing process, therefore cryoprotectant agents such as saccharides are required as protective agents. This study aimed to investigate the effect of various types of saccharides on the quality of frozen semen of etawah crossbreed (PE) bucks during cryopreservation and thawing with plasma replacement using Priangan ram semen plasma. Semen was collected using an artificial vagina once a week. Fresh PE bucks semen centrifuged at 3,000 RPM for 30 min. The supernatant (seminal plasma) was removed and replaced in equal volume with Priangan ram seminal plasma. Semen was divided into four treatments: goat semen with sheep semen plasma in Tris diluent (control); control with 0.6% dextrose added (monosaccharides); control with 0.6% sucrose added (disaccharides); control with 0.6% raffinose added (trisaccharides). Semen quality including percentages of motile spermatozoa, live spermatozoa, and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawing, respectively. Results of this study showed that viability and IPM of monosaccharides, disaccharides, and trisaccharides were significantly (P<0.05) higher than control (54.40 and 51.40; 55.00 and 53.60; 55.60 and 52.20 vs 48.40 and 52.20, respectively). The motility of disaccharides was significantly (P<0.05) higher compared to control and other diluent (47.00 vs 41.00, respectively). In conclusion, different types of saccharides were effective in maintaining the quality of etawah crossbreed buck frozen semen. The addition of disaccharides seems more effective compared to monosaccharides and trisaccharides in tris extender on the quality of etawah crossbreed buck frozen semen.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.89390
Issue No: Vol. 48, No. 2 (2024)
- Effects of Dietary Inclusion of Angelica gigas Nakai root extract on the
Growth Performance, Hematological and Serum Biochemical Parameters in
Broilers
Authors: Joseph Flores dela Cruz, listya Purnamasari, Seong Gu Hwang
Pages: 91 - 98
Abstract: The study aimed to evaluate the impact of dietary supplementation with Angelica gigas Nakai (AGN) root extract on growth performance, hematological indices, and serum biochemical parameters in broiler chickens. A total of 320 straight-run Cobb broiler chicks from a commercial hatchery were distributed among four treatment groups: Basal diet (BD) as the Control; Treatment 1 (T1): BD + 2 g/kg AGN; Treatment 2 (T2): BD + 4 g/kg AGN; and Treatment 3 (T3): BD + 8 g/kg AGN), each comprising eight replicates with 10 birds per replicate. The supplementation of AGN resulted in dose-dependent improvements (P < 0.05) in body weight, gain, and feed efficiency. On both day 21 and day 35, increasing AGN dosage in the diet led to a significantly higher (P < 0.05) values of red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb), and packed cell volume (PCV). By day 21, AGN supplementation dose-dependently decreased (P < 0.05) serum alkaline phosphatase (ALP), aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), urea, and creatinine levels. Meanwhile, AGN dose escalation correlated with a notable increase (P < 0.05) in serum total protein (TP), albumin, and globulin levels. On day 35, increasing AGN levels led to a significant reduction (P < 0.05) in serum AST and ALT activity, along with lowered serum glucose, cholesterol, triglycerides, urea, and creatinine levels. In conclusion, AGN supplementation enhanced growth performance and positively influenced hematological indices and serum biochemistry profiles in broiler chickens. The study confirms the safe and effective utilization of AGN at an 8 g/kg (0.8 %) feed additive dosage to optimize broiler performance. These findings provide valuable insights into the potential benefits of AGN in poultry nutrition.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.91533
Issue No: Vol. 48, No. 2 (2024)
- Characteristic Morphology and Biomass Production of Napier Grass
(Pennisetum purpureum) Cultivar Gama Umami Under Teak Tree (Tectona
grandis) Shade in Blora, Central Java
Authors: Muhammad Zayyan Muafi, Nafiatul Umami, Bambang Suhartanto, Bambang Suwignyo, Miftahush Shirotul Haq, Nilo Suseno, Widiyatno Widiyatno, Leisa Armstrong, David Cook, Yogi Sidik Prasojo
Pages: 99 - 105
Abstract: This study aims to determine the characteristic morphology and biomass production of Napier grass (Pennisetum purpureum) cultivar Gama Umami under teak tree (Tectona grandis) shade in teak tree forest area of Blora, Central Java. The grass was planted using stem cuttings and maintained for four months using the randomized block design (RBD) method with six repetitions in each treatment. This study consisted of two treatments, namely shade under teak tree stands and non-shade or open area as a control. Maintenance was carried out for 14 weeks with the addition of fertilizers, such as NPK (nitrogen, phosphor, and potassium) and urea. Parameters measured included plant height, leaf length, leaf width, first collar length, number of tiller, and stem diameter. After 14 weeks the plants were harvested, and biomass production data were obtained. The results showed that there were no differences in leaf length, leaf width, first collar length, number shoots, and stem diameter between under the shade and non-shade of teak trees. However, the plant height and biomass production of Napier grass cultivated in non-shade teak trees were higher than under the shade of teak trees. The finding showed that Napier grass cultivar Gama Umami could be cultivated under the shade of teak tree forest and introduce for silvopasture system in teak trees forest area.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.95016
Issue No: Vol. 48, No. 2 (2024)
- Polymorphism of Cyt-b Gene in Several Indonesian Cattle Using PCR-RFLP
Method
Authors: Paskah Partogi Agung, Dyah Perwitasari, Achmad Farajallah, Syahruddin Said, Ekayanti Mulyawati Kaiin, Ferdy Saputra, Alfandy Hermansyah, Kuswati Kuswati, Tri Eko Susilorini, Moch. Syamsul Arifin Zein
Pages: 106 - 110
Abstract: The cytochrome b (Cyt-b) gene is one of the genes that is located in the mitochondrial DNA. Variations in the Cyt-b gene can be used to compare different animal species to investigate the origin of certain animal species. This study aimed to assess the genetic diversity of Indonesian local cattle breeds, including Bali cattle as an Indonesian native cattle breed and Banteng as the wild type of Bali cattle, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A total of 336 individual DNA samples from Indonesian cattle breeds were analyzed in this study. The RFLP method using three restriction enzymes, i.e., HinfI, HaeIII, or XbaI, was used to identify the variation of the Cyt-b gene. The Cyt-b gene was polymorphic based on the PCR-RFLP method. There were six alleles of the Cyt-b gene found in this study, i.e., A and B allele (HinfI), C and D allele (HaeIII), X and Y allele (XbaI). All alleles can be found in Pasundan, Madura, and PO cattle. Pesisir cattle have the highest allele frequency D. The UPGMA results showed three clusters of Indonesian native cattle in this study. Cluster 1 consists of Pasundan, Banteng, and Bali cattle. Cluster 2 consists of Madura, PO, and SO cattle. Pesisir was separated from other cattle in cluster 3. The X allele could become an indicator to distinguish Banteng and Bali cattle.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.87484
Issue No: Vol. 48, No. 2 (2024)
- Supplementation of Sakura Block Plus in Beef Cattle Fed with Palm Fronds
and Its Effect on Nutrient Digestibility
Authors: jarmuji jarmuji, Irma Badarina, Lili Warly
Pages: 111 - 116
Abstract: Sakura Block Plus is a modification of Sakura Block, incorporating 6% earthworms and palm kernel cake as substitutes for corn. The objective of this study was to evaluate the supplementation of Sakura Block Plus in palm frond feed on the nutrient digestibility of beef cattle. The research design employed a Latin Square Design (LSD) with treatments P0 (10% Sakura Block Plus), P1 (8% Sakura Block Plus), P2 (10% Sakura Block Plus), and P3 (12% Sakura Block Plus). Four male beef cattle, 18 months old, weighing approximately ± 110 kg, were utilized throughout four treatment periods, each lasting 15 days. The results of the study indicated a significant improvement in the 12% Sakura Block Plus treatment concerning the digestibility of Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), and hemicellulose. In conclusion, the supplementation of Sakura Block Plus at the 12% level in palm frond feed resulted in the most optimal nutrient digestibility.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.88451
Issue No: Vol. 48, No. 2 (2024)
- Analysis of the Nutritional Quality of Local Feed Ingredients Commonly
Used in the Concentrate Formula for Beef Cattle Feedlots in Indonesia
Authors: Thoriqul Irfah Al Huda, Ali Agus, Cuk Tri Noviandi, Siti Andarwati, Andriyani Astuti
Pages: 117 - 127
Abstract: Indonesia's annual beef consumption is experiencing a steady rise. In 2021, the per capita beef consumption amounted to 2.56 kg per person per year, resulting in a total of 696 million kilograms consumed across the country. This quantity is equivalent to the slaughtering of approximately 3.98 million cattle annually. With the increasing growth of the feedlot industry, cattle farmers, including feedlot operators, have the opportunity to enhance the value of agricultural companies in Indonesia by utilizing local feedstuffs. The objective of this study was to assess the suitability and nutritional value of indigenous feed sources for beef cattle in Indonesia. The research utilized feedstuff varieties and characteristics sourced from feedlots spanning since 2012-2021. The employed methodologies encompassed surveys, interviews, and questionnaires. This research involved the collection of both primary and secondary data. The potential and quality of local feedstuff were described using descriptive analysis. This research showed that eight types of local feedstuff could be categorized as energy sources including dehydrated cassava chips with a total digestible nutrients (TDN) value of 84.2% and bran pollard with a TDN value of 66.6%. The fiber sources consisted of corn cob (37.7% crude fiber), coffee husk (38% crude fiber), cocoa bean shell (20% crude fiber), tapioca solid waste/onggok (22% crude fiber), and palm kernel meal (22.1% crude fiber). The protein source consisted of copra meal with a protein content of 22.4%. The physical test most frequently inspected the feed color (18.1%) and odor (18.1%). Moisture examination (24%) was the most frequently used of proximate analysis was employed to identify the chemical composition. In conclusion the existence of eight local ingredients which were categorized into three different types: energy source, protein source, and fiber source commonly used in the concentrate formula for beef cattle feedlots in Indonesia.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.90285
Issue No: Vol. 48, No. 2 (2024)
- Fermentation Products, Microbial Protein Synthesis, and Hydrolytic Enzymes
Activity of Rumen Fluids Local Beef Cattle Supplemented by Direct-Fed
Microbials and Waru Leaf Flour
Authors: Dhany Nursetiyadi, Muhammad Bata, Sri Rahayu
Pages: 128 - 137
Abstract: The aim of this study was to evaluate the effect of Waru Leaf Flour (WLF) and Direct-Fed Microbials (DFM) supplementation on volatile fatty acid (VFA) and ammonia (N-NH3) concentrations, total protozoa, microbial protein synthesis (MPS), and the specific activity of cellulase, amylase, protease in rumen fluids of local beef cattle. The study was conducted as an experimental investigation using a factorial Completely Randomized Design (FCRD). The first factor were three levels of DFM (P) mixed with rice straw (0%, 0.5%, and 1% dry matter of rice straw). The second factor consisted of three levels of WLF supplementation (D) in concentrates (0%, 0.24%, and 0.48% of dry matter concentrate). This resulted nine treatment groups, each it replicated three times.The diet composition consisted of rice straw and concentrate at 40:60 ratio (% dry matter). The interaction between DFM and WLF had a statistically significant impact (p<0.01) on the rumen fluid's total protozoa, N-NH3, and protein content. However, this interaction was not statistically significant (P>0.05) in relation to VFA, MPS, cellulase, amylase, and protease activity. While no significant interaction were observed for total VFA, cellulase, amylase, and protease, DFM supplementation had a statistically significant effect (P<0.01) on reducing enzymes activity, and total VFA (P<0.05). SDS-PAGE and zymography analyzes showed two protease molecules at all treatments with molecular weight of 144 kDa and 133 kDa, cellulase molecules at 62-67 kDa and 19-21 kDa respectively. The addition of DFM showed a new protease band, they had a measurement of 14 and 25 kDa.This study provides valuable insights into enzyme activities in rumen, particularly protease, cellulase, and amylase. The results suggest that the combination of 0.24% WLF and 0.5% DFM supplementation holds promise for optimizing rumen conditions and improving the performance of local beef cattle.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.90556
Issue No: Vol. 48, No. 2 (2024)
- Influence of Retanning Agents on Physical Characteristics of Snow-White
Glove Leather from Goat Skin
Authors: Ragil Yuliatmo, Tutik Maryati, Atiqa Rahmawati, Dahiru J M Adamu, Al Mizan
Pages: 138 - 145
Abstract: The quality of leather is subject to various elements, such as the specific raw material used, and the processing techniques implemented throughout the production process. Goatskin, renowned for its exceptional softness and durability, is frequently employed in the production of gloves owing to its desired attributes, notably its capacity to produce snow-white gloves. Retanning is an essential step in the leather manufacturing process as it significantly improves the characteristics of the leather. Through an investigation into the impacts of distinct retanning agents on goatskin leather gloves, this research endeavor seeks to offer significant knowledge regarding the most effective retanning procedures that can augment the tactile attributes of snow- white leather gloves. In this study, formaldehyde, alum, chromium, and chromium alum were employed as retanning agents. The retanned leathers were assessed by physical parameters, Principal Component Analysis (PCA), and Fourier Transform Infrared spectroscopy (FTIR), then compared to a commercially available snow-white leather glove. The physical characteristics of chrome alum retanned leather showed a high degree of softness (6.60±0.02 mm), good tear strength (1.530±117 N/cm), and tensile strength (1.500±100 N/cm 2 ). The principal component analysis (PCA) also supported that the properties of chrome alum retanned snow-white glove leather closely resemble commercial leather and the result from this study suggested that all factors notably impact the skin's physical quality, except for thickness. Additionally, the FTIR analysis reveals similar functional groups from the different retanned leathers indicated by comparable peaks and stretching patterns. Therefore, chrome-alum could be a well alternative retanning agent for the production of snow-white glove leather.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.95274
Issue No: Vol. 48, No. 2 (2024)
- Food Hygiene-Sanitation Labels: Is It Important' Analysis of Consumer
Attention and Perception Across Age, Gender, Education and Income Groups
Authors: Candra Pungki Wibowo, Suci Paramitasari Syahlani; Ph.D., Fransiskus Trisakti Haryadi
Pages: 146 - 152
Abstract: The suitability of a product's perceived value to consumers’ needs determines the success of offerings. Food safety labels as product attributes have not yet become a concern for most consumers. This research analyzed consumer attention to NKV certification and perceptions of choosing NKV-certified livestock food products regarding demographic profiles. This research was conducted using a survey and data collection was carried out online via social media between June and August 2023. Respondents were determined using a purposive sampling method with the criteria that they had ever shopped for processed livestock products in the last month and were over 18 years old. A total of 210 data were collected and analyzed using descriptive statistical methods and non-parametric Chi-Square. The results showed that behavior regarding NKV certification is positively related to age, education level, and income variables but not to gender. Further analysis showed that the perception of purchasing NKV products is wise and a first choice is greater in groups with older age and higher education levels. Furthermore, the perception of this purchase has the potential to occur in the group of respondents with an income of at least IDR 1,500,000. Furthermore, NKV has not been considered by all respondents, only 14.28% of respondents thought NKV was an important attribute. This study concluded that demographic variables such as age, education level, and income should be deliberated in profiling the market segment for NKV-certified livestock food products. Behavior to purchase NKV products increases in older age groups, higher levels of education, and income.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.91574
Issue No: Vol. 48, No. 2 (2024)
- Technological Acceptance of Cattle Farmers in Mobile Applications for
Livestock Digital Marketing
Authors: Agung Triatmojo, Nguyen Hoang Qui, Yasser Basstawy El Sayed, Mujtahidah Anggriani Ummul Muzayyanah, Suci Paramitasari Syahlani, Budi Guntoro
Pages: 153 - 161
Abstract: The farmers have encountered challenges in conducting livestock trade due to the absence of dealer activity caused by Anthrax and Foot Mouth Disease (FMD) epidemics. In this context, it is crucial to utilize technology in livestock marketing to obtain current market information from distant marketplaces and reduce the risk of contagion. To meet these purposes, a mobile phone application has been developed in order to be used by cattle farmers; after that, market testing has been conducted to gain feedback and determine the segmentation. Thus, the study aimed to examine the differences in the perceived ease of use, perceived usefulness, and social impact amongst farmers who are willing and unwilling to embrace a mobile phone application for digital marketing. A total of 968 cattle farmers were surveyed with stratified random sampling techniques in the Special Region of Yogyakarta. The data obtained were analyzed using mean difference inferential analysis. The result showed that farmers with various categories of age, education, farm revenue, farmers group, farmer experience, cattle ownership, and regions have significantly different (p<0.01) perceived usefulness (PU), perceived ease of use (PE), and social influence (SI) on mobile applications for livestock digital marketing. Furthermore, farmers willing to adopt mobile application have significantly higher (p<0.01) PU, PE, and SI factors. This study recommends mobile app developers evaluate potential user needs and background factors that may influence farmers' interest.
PubDate: 2024-05-31
DOI: 10.21059/buletinpeternak.v48i2.92075
Issue No: Vol. 48, No. 2 (2024)
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