Subjects -> HEALTH AND SAFETY (Total: 1464 journals)
    - CIVIL DEFENSE (22 journals)
    - DRUG ABUSE AND ALCOHOLISM (87 journals)
    - HEALTH AND SAFETY (686 journals)
    - HEALTH FACILITIES AND ADMINISTRATION (358 journals)
    - OCCUPATIONAL HEALTH AND SAFETY (112 journals)
    - PHYSICAL FITNESS AND HYGIENE (117 journals)
    - WOMEN'S HEALTH (82 journals)

PHYSICAL FITNESS AND HYGIENE (117 journals)                     

Showing 1 - 116 of 116 Journals sorted alphabetically
ACSMs Health & Fitness Journal     Full-text available via subscription   (Followers: 14)
Acta Facultatis Educationis Physicae Universitatis Comenianae     Open Access   (Followers: 3)
Acta Kinesiologiae Universitatis Tartuensis     Open Access   (Followers: 1)
ACTIVE : Journal of Physical Education, Sport, Health and Recreation     Open Access   (Followers: 30)
Adapted Physical Activity Quarterly     Hybrid Journal   (Followers: 4)
Ágora para la Educación Física y el Deporte     Open Access  
Al.Qadisiya journal for the Sciences of Physical Education     Open Access  
American Journal of Sexuality Education     Hybrid Journal   (Followers: 4)
Annals of Applied Sport Science     Open Access   (Followers: 11)
Annals of Work Exposures and Health     Hybrid Journal   (Followers: 9)
Applied Physiology, Nutrition and Metabolism     Hybrid Journal   (Followers: 37)
Arab Journal of Nutrition and Exercise     Open Access  
Arquivos em Movimento     Open Access   (Followers: 1)
Arrancada     Open Access  
Asian Journal of Sport and Exercise Psychology     Open Access   (Followers: 4)
Baltic Journal of Sport and Health Sciences     Open Access   (Followers: 2)
BMC Obesity     Open Access   (Followers: 8)
BMC Sports Science, Medicine and Rehabilitation     Open Access   (Followers: 37)
Child and Adolescent Obesity     Open Access   (Followers: 3)
Childhood Obesity     Hybrid Journal   (Followers: 24)
Clinical Journal of Sport Medicine     Hybrid Journal   (Followers: 37)
Comparative Exercise Physiology     Hybrid Journal   (Followers: 21)
Cultura, Ciencia y Deporte     Open Access   (Followers: 1)
Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity     Hybrid Journal   (Followers: 25)
eJRIEPS : Ejournal de la recherche sur l'intervention en éducation physique et sport     Open Access  
Environmental Health and Preventive Medicine     Open Access   (Followers: 4)
Éthique & Santé     Full-text available via subscription  
Fat Studies : An Interdisciplinary Journal of Body Weight and Society     Partially Free   (Followers: 2)
Food Science and Human Wellness     Open Access   (Followers: 4)
Frontiers in Sports and Active Living     Open Access   (Followers: 2)
Gelanggang Pendidikan Jasmani Indonesia     Open Access  
German Journal of Exercise and Sport Research : Sportwissenschaft     Hybrid Journal   (Followers: 2)
Geron     Full-text available via subscription  
Health and Quality of Life Outcomes     Open Access   (Followers: 14)
Health Education     Hybrid Journal   (Followers: 1)
Health Education Journal     Hybrid Journal   (Followers: 16)
Health Marketing Quarterly     Hybrid Journal   (Followers: 3)
Health Physics     Hybrid Journal   (Followers: 7)
Home Healthcare Now     Hybrid Journal   (Followers: 4)
Human Movement Science     Hybrid Journal   (Followers: 14)
IISE Transactions on Occupational Ergonomics and Human Factors     Hybrid Journal  
Indonesia Performance Journal     Open Access  
International Journal for Vitamin and Nutrition Research     Hybrid Journal   (Followers: 10)
International Journal of Applied Exercise Physiology     Open Access   (Followers: 54)
International Journal of Athletic Therapy & Training     Hybrid Journal   (Followers: 15)
International Journal of Behavioral Nutrition and Physical Activity     Open Access   (Followers: 31)
International Journal of Obesity     Hybrid Journal   (Followers: 93)
International Journal of Obesity Supplements     Full-text available via subscription   (Followers: 7)
International Journal of Qualitative Studies on Health and Well-Being     Open Access   (Followers: 21)
International Journal of Spa and Wellness     Hybrid Journal  
International Journal of Sport, Exercise & Training Sciences     Open Access   (Followers: 3)
International Journal of Yoga     Open Access   (Followers: 17)
Isokinetics and Exercise Science     Hybrid Journal   (Followers: 10)
Journal of Advanced Nutrition and Human Metabolism     Open Access   (Followers: 16)
Journal of American College Health     Hybrid Journal   (Followers: 3)
Journal of Athlete Development and Experience     Open Access   (Followers: 2)
Journal of Bioenergetics and Biomembranes     Hybrid Journal   (Followers: 1)
Journal of Exercise & Organ Cross Talk     Open Access   (Followers: 3)
Journal of Human Performance in Extreme Environments     Open Access   (Followers: 2)
Journal of Human Sport and Exercise     Open Access   (Followers: 16)
Journal of Motor Learning and Development     Hybrid Journal  
Journal of Physical Activity and Health     Hybrid Journal   (Followers: 11)
Journal of Physical Activity and Hormones     Open Access   (Followers: 2)
Journal of Physical Activity Research     Open Access   (Followers: 3)
Journal of Physical Education and Human Movement     Open Access  
Journal of Physical Education and Sport Sciences     Open Access   (Followers: 3)
Journal of Physical Education Health and Sport     Open Access   (Followers: 2)
Journal of Physical Education, Recreation & Dance     Full-text available via subscription   (Followers: 11)
Journal of Science in Sport and Exercise     Hybrid Journal   (Followers: 4)
Journal of Sport and Health Science     Open Access   (Followers: 23)
Journal of Sport Sciences and Fitness     Open Access   (Followers: 13)
Journal of Strength and Conditioning Research     Hybrid Journal   (Followers: 73)
Kinesiology : International Journal of Fundamental and Applied Kinesiology     Open Access   (Followers: 1)
Kinesiology Review     Hybrid Journal   (Followers: 4)
Malaysian Journal of Movement, Health & Exercise     Open Access   (Followers: 3)
Measurement in Physical Education and Exercise Science     Hybrid Journal   (Followers: 7)
Médecine & Nutrition     Full-text available via subscription  
Mental Health and Physical Activity     Hybrid Journal   (Followers: 16)
MHSalud : Movimiento Humano y Salud     Open Access  
Obesity     Hybrid Journal   (Followers: 58)
Obesity Research & Clinical Practice     Full-text available via subscription   (Followers: 20)
Obesity Reviews     Hybrid Journal   (Followers: 25)
Obesity Science & Practice     Open Access   (Followers: 1)
Open Obesity Journal     Open Access   (Followers: 1)
Pain Management in General Practice     Full-text available via subscription   (Followers: 12)
PALAESTRA : Adapted Sport, Physical Education, and Recreational Therapy     Full-text available via subscription   (Followers: 3)
Physical Activity and Health     Open Access   (Followers: 3)
Physical Education & Sport Pedagogy     Hybrid Journal   (Followers: 13)
Preventing Chronic Disease     Free   (Followers: 3)
Psychology of Sport and Exercise     Hybrid Journal   (Followers: 18)
Quality in Sport     Open Access  
Race and Yoga     Open Access  
RBNE - Revista Brasileira de Nutrição Esportiva     Open Access   (Followers: 1)
RBONE - Revista Brasileira de Obesidade, Nutrição e Emagrecimento     Open Access   (Followers: 1)
RBPFEX - Revista Brasileira de Prescrição e Fisiologia do Exercício     Open Access  
Research Quarterly for Exercise and Sport     Hybrid Journal   (Followers: 2)
Retos : Nuevas Tendencias en Educación Física, Deportes y Recreación     Open Access  
Revista Andaluza de Medicina del Deporte     Open Access   (Followers: 2)
Revista Brasileira de Atividade Física & Saúde     Open Access   (Followers: 1)
Revista Brasileira de Cineantropometria & Desempenho Humano     Open Access   (Followers: 1)
Revista Internacional de Medicina y Ciencias de la Actividad Física y del Deporte : International Journal of Medicine and Science of Physical Activity and Sport     Open Access   (Followers: 1)
Revue phénEPS / PHEnex Journal     Open Access  
Scandinavian Journal of Sport and Exercise Psychology     Open Access   (Followers: 3)
SIPATAHOENAN : South-East Asian Journal for Youth, Sports & Health Education     Open Access  
Spor Bilimleri Dergisi / Hacettepe Journal of Sport Sciences     Open Access  
Sport and Fitness Journal     Open Access   (Followers: 6)
Sport Science and Health     Open Access   (Followers: 2)
Sport Sciences for Health     Hybrid Journal   (Followers: 5)
SPORTIVE : Journal Of Physical Education, Sport and Recreation     Open Access  
Sports     Open Access   (Followers: 3)
Sports Biomechanics     Hybrid Journal   (Followers: 27)
Sports Health: A Multidisciplinary Approach     Hybrid Journal   (Followers: 4)
Strength & Conditioning Journal     Hybrid Journal   (Followers: 56)
Timisoara Physical Education and Rehabilitation Journal     Open Access   (Followers: 1)
Turkish Journal of Sport and Exercise     Open Access  
Yoga Mimamsa     Open Access   (Followers: 3)

           

Similar Journals
Journal Cover
Journal of Bioenergetics and Biomembranes
Journal Prestige (SJR): 1.033
Citation Impact (citeScore): 2
Number of Followers: 1  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1573-6881 - ISSN (Online) 0145-479X
Published by Springer-Verlag Homepage  [2467 journals]
  • Separation and analysis of Bacillus subtilis respiratory chain complexes

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      Abstract: Abstract Bacillus subtilis is a Gram-positive bacterium with a respiratory chain embedded in the cytoplasmic membrane. The respiratory chain is bifurcated after menaquinol into a cytochrome b6c + caa3 branch and a branch with up to three quinol oxidases. The complexes that generate the proton gradient are b6c, associated with caa3 and aa3 oxidase. The b6c and caa3 complexes form a supercomplex, and it is proposed to form respiratory strings in the membrane. There is still information missing about the quinol branch and if the primary oxidase quinol aa3 is associated with the electron donor complexes. It is unclear whether succinate quinone reductase (SQR) can form associations with the quinol branch or the cytochrome branch. In this paper, we show the separation of an almost pure b6c complex associated with cytochromes c550 and c551. We obtained a b6c + caa3 supercomplex of 600 kDa and SQR, aa3, and NADH dehydrogenase by dodecyl maltoside solubilization and separation of the respiratory chain components by ionic exchange chromatography. We found that aa3 does not associate with other complexes. SQR was associated with the b6c complex in a mutant lacking aa3. This association could facilitate electron transfer from SQR to menaquinone-7. The lack of associations between the abundant quinol oxidase aa3 and other complexes is a feature we cannot explain yet.
      PubDate: 2022-11-23
       
  • Effect of H2O2 on Na,K-ATPase

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      Abstract: Abstract Na,K-ATPase is a member of the P-type ATPase family, which transforms the energy of ATP to the transmembrane Na/K gradient that is used to create membrane potential, support the excitability of neurons and myocytes, control pH, and transport substances. The regulation of the Na,K-ATPase function by physiological regulators also comprises a central role in the adaptation of organisms to different conditions. H2O2 is one of the main signaling molecules in redox metabolism and plays important function in cellular physiology. H2O2 also regulates signaling pathways via the specific oxidation of proteins harboring redox-sensitive moieties, like metal centers or cysteine residues, which control their activity. The Na,K-ATPase is redox-sensitive with an “optimal redox potential range,” where the reactive oxygen species (ROS), levels beyond this “optimal range” are responsible for enzyme inhibition. Thus reactive oxygen species manifest a hermetic effect, which is expressed by biphasic action; stimulation by low doses and inhibition by high doses. This study was aimed to reveal redox-sensitivity of brain synaptic membrane fractions Na,K-ATPase via H2O2 effects. Different concentrations of H2O2 change the kinetic parameters of the enzyme system for MgATP complex, Na+, and K+ differently. Moreover, H2O2 changes p-chloromercuribenzoic acids (PCMB) affinity. H2O2 targets thiols of the Na,K-ATPase – low and high concentrations of H2O2 change the oxidative state of thiolate (S‐) from Cys differently, resulting in the corresponding activation or inhibition of the enzyme. Targeting thiols of the Na,K-ATPase tunes the activity of the Na,K-ATPase to the cellular demands and sustains the enzyme activity at the “optimal” level.
      PubDate: 2022-11-04
       
  • NDUFA4 promotes cell proliferation by enhancing oxidative phosphorylation
           in pancreatic adenocarcinoma

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      Abstract: Abstract Pancreatic adenocarcinoma (PAAD) is the third leading cause of cancer-related deaths, with a 5-year relative survival rate of 6%. Hence, novel therapeutic targets need to be urgently explored for PAAD. Recently, oxidative phosphorylation (OXPHOS) has been identified to contribute to the development of PAAD. Nicotinamide adenine dinucleotide + hydrogen (NADH) dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4) is known to affect the mitochondrial respiration pathway. However, the function of NDUFA4 in PAAD remains unclear. In this study, NDUFA4 expression was examined in samples from patients with PAAD using real-time polymerase chain reaction and immunohistochemical staining. Furthermore, cell proliferation and cell cycle were analyzed using Cell Counting Kit-8 assay and flow cytometry. A xenograft tumor model derived from a PAAD cell line was developed to validate the in vitro findings. NDUFA4 was observed to be upregulated in the PAAD samples, and high levels were associated with a poor survival rate. NDUFA4 knockdown reduced cell proliferation by inducing G1 arrest in SW1990 cells. Mechanistically, NDUFA4 knockdown decreased the oxygen consumption rate, cellular adenosine triphosphate level, mitochondrial complex IV activity, and protein levels of COX6C and COX5B, which indicated the suppression of OXPHOS. In contrast, NDUFA4 overexpression exerted the opposite effects. Finally, NDUFA4 knockdown significantly inhibited the growth of the xenograft tumor derived from the SW1990 cell line in vivo. Therefore, NDUFA4 contributes to PAAD proliferation by enhancing OXPHOS.
      PubDate: 2022-10-29
       
  • Isolation and characterization of a main porin from the outer membrane of
           Salinibacter ruber

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      Abstract: Abstract Salinibacter ruber is an extremophilic bacterium able to grow in high-salts environments, such as saltern crystallizer ponds. This halophilic bacterium is red-pigmented due to the production of several carotenoids and their derivatives. Two of these pigment molecules, salinixanthin and retinal, are reported to be essential cofactors of the xanthorhodopsin, a light-driven proton pump unique to this bacterium. Here, we isolate and characterize an outer membrane porin-like protein that retains salinixanthin. The characterization by mass spectrometry identified an unknown protein whose structure, predicted by AlphaFold, consists of a 8 strands beta-barrel transmembrane organization typical of porins. The protein is found to be part of a functional network clearly involved in the outer membrane trafficking. Cryo-EM micrographs showed the shape and dimensions of a particle comparable with the ones of the predicted structure. Functional implications, with respect to the high representativity of this protein in the outer membrane fraction, are discussed considering its possible role in primary functions such as the nutrients uptake and the homeostatic balance. Finally, also a possible involvement in balancing the charge perturbation associated with the xanthorhodopsin and ATP synthase activities is considered.
      PubDate: 2022-10-13
       
  • Synthesis, biological evaluation and molecular modeling studies of novel
           1,2,3-triazole-linked menadione-furan derivatives as P2X7 inhibitors

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      Abstract: Abstract The P2X7 receptor (P2X7R) is an ion channel that promotes the passage of ions through the membrane through brief stimulation once activated by ATP, its endogenous opener. However, prolonged stimulation with ATP, which occurs in pathological processes, opens a nonselective pore in the plasma membrane, allowing the passage of large molecules and leading to cytokine release or even cell death. In this sense, the search for new inhibitors for this receptor has attracted a great deal of attention in recent years. Considering the booming of biomass upgrading reactions in recent years and the continued efforts to synthesize biologically active molecules containing the 1,2,3-triazole ring, in the present work, we aimed to investigate whether triazole-linked menadione-furan derivatives could present P2X7R inhibitory activity. The novel compounds were tested for their inhibitory activity on ATP-induced dye uptake in peritoneal macrophages. Some have shown promising results, having displayed IC50 values lower than that of the P2X7R inhibitor BBG. Molecular docking studies also indicated that the active compounds bind to an allosteric site on P2X7R, presenting potential P2X7R inhibition.
      PubDate: 2022-09-07
      DOI: 10.1007/s10863-022-09947-2
       
  • CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via
           the miR-140-5p/MLKL axis

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      Abstract: Abstract Circular RNAs (circRNAs) play vital roles in human diseases, including acute kidney injury (AKI). In this paper, we focused on the effect of circRNA zinc finger protein 644 (circZNF644) on AKI cell model progression. qRT-PCR was conducted for the levels of circZNF644, ZNF644, miR-140-5p and mixed lineage kinase domain like pseudokinase (MLKL). RNase R assay, actinomycin D assay and subcellular fraction analysis were conducted to analyze the features of circZNF644. CCK-8 assay and EdU assay were used to explore cell proliferation. Flow cytometry analysis was conducted to analyze cell cycle and cell apoptosis. Western blot assay was executed for protein levels. ELISA was performed for the levels of inflammatory cytokines. The relationships among circZNF644, miR-140-5p and MLKL were analyzed by dual-luciferase reporter assay and RIP assay. CircZNF644 was upregulated in LPS-stimulated HK-2 cells. LPS-mediated inhibitory effects on cell proliferation and cell cycle and promotional effects on apoptosis and inflammation were reversed by circZNF644 knockdown. CircZNF644 directly interacted with miR-140-5p and MLKL was the target gene of miR-140-5p. The impact of circZNF644 knockdown on HK-2 cell injury was relieved by miR-140-5p inhibition. Moreover, miR-140-5p enhancement alleviated LPS-triggered HK-2 cell damage, while MLKL elevation reversed the effect. CircZNF644 knockdown protected HK-2 cells from LPS-induced injury by altering miR-140-5p/MLKL pathway, suggesting that circZNF644 may be a hopeful therapeutic target for AKI.
      PubDate: 2022-08-17
      DOI: 10.1007/s10863-022-09946-3
       
  • Disruption of mitochondrial functions involving mitochondrial permeability
           transition pore opening caused by maleic acid in rat kidney

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      Abstract: Abstract Propionic acid (PA) predominantly accumulates in tissues and biological fluids of patients affected by propionic acidemia that may manifest chronic renal failure along development. High urinary excretion of maleic acid (MA) has also been described. Considering that the underlying mechanisms of renal dysfunction in this disorder are poorly known, the present work investigated the effects of PA and MA (1–5 mM) on mitochondrial functions and cellular viability in rat kidney and cultured human embryonic kidney (HEK-293) cells. Mitochondrial membrane potential (∆ψm), NAD(P)H content, swelling and ATP production were measured in rat kidney mitochondrial preparations supported by glutamate or glutamate plus malate, in the presence or absence of Ca2+. MTT reduction and propidium iodide (PI) incorporation were also determined in intact renal cells pre-incubated with MA or PA for 24 h. MA decreased Δψm and NAD(P)H content and induced swelling in Ca2+-loaded mitochondria either respiring with glutamate or glutamate plus malate. Noteworthy, these alterations were fully prevented by cyclosporin A plus ADP, suggesting the involvement of mitochondrial permeability transition (mPT). MA also markedly inhibited ATP synthesis in kidney mitochondria using the same substrates, implying a strong bioenergetics impairment. In contrast, PA only caused milder changes in these parameters. Finally, MA decreased MTT reduction and increased PI incorporation in intact HEK-293 cells, indicating a possible association between mitochondrial dysfunction and cell death in an intact cell system. It is therefore presumed that the MA-induced disruption of mitochondrial functions involving mPT pore opening may be involved in the chronic renal failure occurring in propionic acidemia.
      PubDate: 2022-07-29
      DOI: 10.1007/s10863-022-09945-4
       
  • The potential role of circulating exosomes in protecting myocardial injury
           in acute myocardial infarction via regulating miR-190a-3p/CXCR4/CXCL12
           pathway

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      Abstract: Abstract Exosomes of different origins have been found to be protective against ischemic-induced myocardial injury. This study examined the protective effects of circulating exosomes in the mice model of acute myocardial infarction (AMI) and explored the underlying molecular mechanisms. The effects of exosomes on myocardial injury were assessed in the AMI mice model. The in vivo studies showed that circulating exosomes reduced the infarcted size, improved the morphology of heart tissues and also reduced apoptosis of the heart tissues. In addition, the model mice showed an increase in the CD34 + /VEGFR2 + cell population and CD31, CXCR4 and CXCL12 expression after exosomes treatment. MiR-190a-3p was significantly down-regulated in the exosomes derived from the culture medium of hypoxia-treated human cardiomyocytes (HCMs). Further analysis revealed that miR-190a-3p could physically interact with CXCR4/CXCL12 by targeting the respective 3’UTRs. These exosomes could up-regulated CXCR4 and CXCL12 expression in the EPCs; in addition, miR-190a-3p mimics repressed CXCR4/CXCL12 expression in EPCs, while its inhibitor had opposite effects. The in vitro functional assays showed that miR-190a-3p overexpression suppressed the cell viability, proliferation, migration, adhesion and tube formation of EPCs; while miR-190a-3p inhibitor had the opposite effects; exosomes derived from the culture medium of hypoxia-treated HCMs exhibited similar actions of miR-190a-3p inhibitor. Moreover, miR-190a-3p was down-regulated in exosomes from serum in the AMI group when compared to that from sham group. Treatment with exosomes from serum in the AMI group promoted cell proliferation, migration, adhesion and tube formation of EPCs when compared to that in the sham group. More importantly, IT1t attenuated the enhanced effects of miR-190a-3p inhibition on EPC proliferation, migration, adhesion and tube formation. In conclusion, circulating exosomes exerted protective effects on myocardial injury in the AMI mice model, and down-regulation of miR-190a-3p in the circulating exosomes may exert protective effects against myocardial injury. Hypoxia induced the downregulation of miR-190a-3p in the culture medium of HCMs, and the mechanistic investigations indicated that exosomes of hypoxia-conditioned HCM culture medium promoted the cell viability, proliferation, migration, adhesion and tube formation of EPCs via regulating miR-190a-3p/CXCR4/CXCL12 pathway.
      PubDate: 2022-07-22
      DOI: 10.1007/s10863-022-09944-5
       
  • Resveratrol regulates the inflammation and oxidative stress of granulosa
           cells in PCOS via targeting TLR2

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      Abstract: Abstract Polycystic ovary syndrome (PCOS) is featured as a common endocrine disorder in reproductive-aged women, while its pathophysiology is not fully illustrated. This study examined potential actions of resveratrol in PCOS cellular model and explored the underlying interaction between resveratrol and toll-like receptor 2 (TLR2). This study performed the bioinformatics analysis on two microarray datasets (GSE34526 and GSE138518). We found that TLR2 was one of potential hub genes that may be associated with PCOS. Further examination showed that TLR2 was highly expressed in granulosa cells from PCOS group compared with control. The in vitro studies showed that LPS intervention caused an increased expression of TLR2 and the pro-inflammatory mediators, and induced oxidative stress in the granulosa cells, which was concentration-dependently antagonized by resveratrol treatment. TLR2 silence significantly attenuated LPS-induced increase TNF-α, IL-1β, IL-6 and IL-8 expression and oxidative stress of granulosa cells. Furthermore, TLR2 overexpression promoted inflammatory response and oxidative stress in the granulosa cells, which was antagonized by resveratrol treatment. In conclusion, resveratrol could attenuate LPS-induced inflammation and oxidative stress in granulosa cells, and the underlying mechanisms may be related to the inhibitory effect of resveratrol on TLR2 expression in granulosa cells.
      PubDate: 2022-07-14
      DOI: 10.1007/s10863-022-09942-7
       
  • Correction to: Chronic exposure to ozone induces cardiac antioxidant
           response and overexpression of either mitochondrial fsion protein DRP1 and
           hipertrophyc-related proteins

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      PubDate: 2022-07-04
      DOI: 10.1007/s10863-022-09943-6
       
  • TLR4 inhibitor alleviates sepsis-induced organ failure by inhibiting
           platelet mtROS production, autophagy, and GPIIb/IIIa expression

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      Abstract: Abstract Thrombocytopenia and impaired platelet function are associated with sepsis-induced organ failure. Numerous studies have shown that mitochondrial ROS (mtROS) and autophagy are related to organ injury in sepsis. However, the relationships between platelet mtROS, autophagy and sepsis organ failure remain unclear. Herein, we explored whether toll like receptor 4 (TLR4) inhibitor alleviates sepsis organ failure by inhibiting platelet mtROS production, autophagy, and GPIIb/IIIa expression. Mice were administrated with LPS, LPS + TAK242 or vehicle. The lungs and kidneys were harvested and analyzed using hematoxylin and eosin staining assay. Platelet rich plasma (PRP) was isolated from blood and platelets aggregation and TLR4 expression were analyzed using flow cytometer and western blot. PRP from healthy volunteers was treated with saline, LPS, or LPS + TAK242, and then mitoSOX and calcium were detected using flow cytometer, and NOX2 and LC3B were tested using western blot. Results showed that TAK242 effectively alleviated LPS-induced acute kidney and lung injury in mice, and decreased CD41 expression more significantly than CD62P. In vitro, by inhibiting TLR4, TAK242 suppressed Ca2+, mitoSOX fluorescence, NOX2 expression and LC3BII/LC3BI ratio in LPS treated platelets. TLR4 inhibitor TAK242 may effectively alleviate mouse lung and kidney injury by inhibition of mouse platelet GPIIb/IIIa, and reduce LPS-induced mtROS generation related to Ca2+ influx, thus reducing platelet activation.
      PubDate: 2022-06-08
      DOI: 10.1007/s10863-022-09940-9
       
  • SKA-31-induced activation of small-conductance calcium-activated potassium
           channels decreased modulation of detrusor smooth muscle function in a rat
           model of obesity

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      Abstract: Abstract Increased excitability and contractility of detrusor smooth muscle (DSM) cells are associated with overactive bladder (OAB), which is often induced by obesity. Small-conductance Ca2+-activated K+ (SK) channels regulate the excitability and contractility of DSM cells. Selective pharmacological activation of SK channels attenuates hyperpolarization and the decreased relaxation effect in DSM cells in obesity-induced OAB. However, additional data are needed to confirm the regulatory effect of SK channels on the function of DSM cells in obesity-related OAB. The tested hypothesis was that activation of SK channels decreases modulation of DSM function in a rat model of obesity-related OAB. Female Sprague Dawley rats were fed a normal diet (ND) or a high-fat diet (HFD), weighed after 12 weeks, and subjected to urodynamic study, patch-clamp electrophysiology, and isometric tension recording. The average body weight and incidence of OAB were increased in the HFD group. Patch-clamp studies revealed that pharmacological activation of SK channels with SKA-31 had attenuated hyperpolarization of DSM cells. In addition, isometric tension recordings indicated that SKA-31 decreased relaxation of spontaneous phasic contractions of DSM strips in the HFD group. Attenuated function of SK channels increased the excitability and contractility of DSM cells, which contributed to the occurrence of OAB, suggesting that SK channels are potential therapeutic targets for control of OAB.
      PubDate: 2022-04-27
      DOI: 10.1007/s10863-022-09939-2
       
  • CircCDC6 restrains tumor growth and glycolysis energy metabolism in
           colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

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      Abstract: Abstract The aberrant downregulation of circCDC6 in colorectal cancer (CRC) was previously identified by circRNA microarray analysis. However, the detailed role of circCDC6 in CRC is still lacking. We thus investigated the function of circCDC6 in CRC. The expression of circCDC6, miR-3187-3p and PRKAA2 mRNA was checked by real-time quantitative PCR (RT-qPCR). Cell growth was evaluated by MTT, EdU and colony formation assays. Cell apoptosis was evaluated by flow cytometry. Glycolysis was evaluated by glycolysis stress test and lactic acid level. The expression of PRKAA2, HK2 and LDHA proteins was checked by western blotting. The potential binding between miR-3187-3p and circCDC6 or PRKAA2 was confirmed by dual-luciferase reporter assay, RIP assay and pull-down assay. Xenograft model was established in nude mice. CircCDC6 showed poor expression in CRC tumor samples and cells. CircCDC6 ectopic expression repressed CRC cell proliferation, survival and glycolysis energy metabolism. MiR-3187-3p was targeted by circCDC6, and miR-3187-3p depletion also repressed CRC cell growth and glycolysis. PRKAA2 was a downstream target of circCDC6/miR-3187-3p pathway, and circCDC6 upregulated PRKAA2 expression via targeting miR-3187-3p. PRKAA2 knockdown rescued the functional effects of circCDC6 ectopic expression. CircCDC6 overexpression in vivo impeded tumor development in animal models. CircCDC6, acting as a tumor inhibitor, repressed tumor growth and glycolysis metabolism in CRC via targeting the miR-3187-3p/PRKAA2 axis, which partly clarified the role of circCDC6 in CRC.
      PubDate: 2022-04-19
      DOI: 10.1007/s10863-022-09938-3
       
  • Chronic exposition to ozone induces cardiac antioxidant response and
           overexpression of either mitochondrial fision protein DRP1 and
           hipertrophyc-related proteins

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      Abstract: Abstract Pollution is considered a risk factor for cardiovascular disease; however, the mechanisms to explain this relationship are not well understood; ozone is one of the most abundant and studied air contaminants. Our study aimed to evaluate the effect of chronic exposition of rats to controlled low doses of ozone on oxidative stress, apoptosis, mitochondrial dynamics, and cardiac hypertrophy. Male Wistar rats were daily exposed to low ozone doses during 7, 15, 30, and 60 days, 4 h/day. Hearts were dissected, and homogenates were prepared. Oxidative stress was evaluated by TBARS and protein nitrosylation in addition to Superoxide dismutase 1 (SOD1) and Catalase levels; the apoptosis related-proteins caspase 3, caspase 9, Bax, Bcl-2, and the mitochondrial dynamic-associated proteins Fis1, Drp1, OPA1, and Mfn1 were quantified by western blot among the cardiac hypertrophy indicator alpha-actin (cardiac actin). There were no changes in the oxidative stress markers, however SOD1 expression increases. Caspase 3 expression decreased, whereas caspase 9 increased without changes in Bax or Bcl-2. Mitochondrial fission may be favored according to the increased expression of Drp1 but not changes in fusion-related proteins OPA1 and Mfn1. Finally, the molecular marker for cardiac hypertrophy was overexpressed after 30 and 60 days of ozone exposition. The chronic exposition to ozone induces a deleterious effect on cardiac mitochondria. Antioxidant defenses also show changes in relation to exposure time, as well as an apparent pro-hypertrophic effect associated with altered mitochondrial dynamics.
      PubDate: 2022-03-26
      DOI: 10.1007/s10863-022-09937-4
       
  • miR-29b ameliorates atrial fibrosis in rats with atrial fibrillation by
           targeting TGFβRΙ and inhibiting the activation of Smad-2/3 pathway

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      Abstract: Objective Atrial fibrillation (AF) is a major cause of stroke with lifetime risks. microRNAs (miRNAs) are associated with AF attenuation, yet the mechanism remains unknown. This study investigated the functional mechanism of miR-29b in atrial fibrosis in AF. Methods The AF rat model was established by a 7-day intravenous injection of Ach-CaCl2 mixture. AF rats were injected with adeno-associated virus (AAv)-miR-29b and TGFβRΙ overexpression plasmid. AF duration was recorded by electrocardiogram. Atrial fibrosis was observed by Masson staining. Expressions of COL1A1, COL3A1, TGFβRΙ, TGFβΙ, miR-29b and Smad-2/3 pathway-related proteins in atrial tissues were detected by RT-qPCR and Western blot. Binding sites of miR-29b and TGFβRΙ were predicted and their target relationship was verified by dual-luciferase reporter assay. Results miR-29b was poorly expressed and expressions of COL1A1, COL3A1, TGFβRΙ, and TGFβ1 were increased in atrial tissues of AF rats. miR-29b overexpression alleviated atrial fibrosis, reduced expressions of COL1A1, COL3A1, and TGFβ1, and shortened AF duration in AF rats. TGFβRΙ was highly expressed in atrial tissues of AF rats. miR-29b targeted TGFβRΙ. TGFβRΙ overexpression overcame the improving effect of miR-29b overexpression on AF. miR-29b overexpression decreased ratios of p-Smad-2/3 and Smad-2/3 and inhibited the Smad-2/3 pathway. Conclusion miR-29b might mitigate atrial fibrosis in AF rats by targeting TGFβRΙ and inhibiting the Smad-2/3 pathway.
      PubDate: 2022-03-24
      DOI: 10.1007/s10863-022-09934-7
       
  • Circ_0001093 promotes glutamine metabolism and cancer progression of
           esophageal squamous cell carcinoma by targeting miR-579-3p/glutaminase
           axis

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      Abstract: Abstract Increasing studies indicate that circular RNAs (circRNAs) play critical roles in tumor metabolism of multiple cancers. However, the contribution of circRNAs in glutamine metabolism of esophageal squamous cell carcinoma (ESCC) remains elusive. The objective of this research was to investigate the role and mechanism of circRNA hsa_circ_0001093 (circ_0001093) in the glutamine metabolism and tumorigenesis of ESCC. Circ_0001093, microRNA-579-3p (miR-579-3p) and glutaminase (GLS) expressions in ESCC tissues and cell lines were measured by qRT-PCR, tissue array or Western blot. Cell proliferation, invasion and migration were assessed by CCK-8 or transwell assays. Glutamine consumption, glutamate and ATP production were detected by indicated assay kits. The relationships between circ_0001093 and miR-579-3p or GLS mRNA were investigated by bioinformatics analysis, RNA pull-down, luciferase reporter and RNA immunoprecipitation (RIP) assays. Here, we found that circ_0001093 expression was up-regulated in ESCC tissues and cell lines. Increased circ_0001093 expression predicted an unfavourable prognosis, and was associated with the lymph node metastasis, TNM staging and tumor size in ESCC tissues. Circ_0001093 knockdown suppressed cell proliferation, invasion, migration and glutamine metabolism of ESCC cells, while circ_0001093 over-expression showed the opposite effects. Mechanistically, circ_0001093 acted as a competing endogenous RNA (ceRNA) by sponging miR-579-3p, thereby increasing GLS expression. Furthermore, the inhibitory effects of circ_0001093 knockdown on the invasion, migration and glutamine metabolism were partly rescued by miR-579-3p inhibition or GLS over-expression in ESCC cells. Additionally, miR-579-3p expression was down-regulated in ESCC tissues, while GLS expression was up-regulated. In conclusion, this study first provides evidence that the circ_0001093/miR-579-3p/GLS regulatory network can affect glutamine metabolism and malignant phenotype of ESCC, which can further impact ESCC progression.
      PubDate: 2022-03-23
      DOI: 10.1007/s10863-022-09935-6
       
  • Correction to: A critique of the capacitor-based “Transmembrane
           Electrostatically Localized Proton” hypothesis

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      PubDate: 2022-03-19
      DOI: 10.1007/s10863-022-09936-5
       
  • Circ-LRP1B functions as a competing endogenous RNA to regulate
           proliferation, apoptosis and oxidative stress of LPS-induced human C28/I2
           chondrocytes

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      Abstract: Abstract Circular RNAs (circRNAs) are crucial for the pathogenesis of human diseases, including osteoarthritis (OA). Here, we set to elucidate the biological action of circ-LRP1B in OA pathogenesis. Human C28/I2 chondrocytes were stimulated by lipopolysaccharide (LPS). Circ-LRP1B, nuclear factor, erythroid 2 like 1 (NRF1) and microRNA (miR)-34a-5p were quantified by quantitative real-time PCR (qRT-PCR) or immunoblotting. Cell viability, proliferation, and apoptosis abilities were gauged by MTT, 5-Ethynyl-2’-Deoxyuridine (EdU) staining, and flow cytometry assays, respectively. Direct relationship between miR-34a-5p and circ-LRP1B or NRF1 was validated by RNA pull-down and dual-luciferase reporter assays. Circ-LRP1B was found to be underexpressed in OA cartilage and LPS-stimulated C28/I2 chondrocytes. Enforced expression of circ-LRP1B promoted cell proliferation, and repressed apoptosis and oxidative stress, as well as impacted OA-specific hallmarks expression of LPS-stimulated C28/I2 cells. NRF1 was identified as a downstream effector of circ-LRP1B function. Moreover, NRF1 was identified as a miR-34a-5p target in LPS-stimulated C28/I2 cells. Circ-LRP1B acted as a competing endogenous RNA (ceRNA) for miR-34a-5p to involve the post-transcriptional regulation of NRF1 expression. Furthermore, the effects of circ-LRP1B overexpression partly depended on the reduction of available miR-34a-5p. These findings demonstrate that circ-LRP1B functions as a ceRNA to regulate the proliferation, apoptosis and oxidative stress of LPS-stimulated human C28/I2 chondrocytes by miR-34a-5p/NRF1 network.
      PubDate: 2022-03-10
      DOI: 10.1007/s10863-022-09932-9
       
  • Effects of lysophosphatidic acid (LPA) signaling via LPA receptors on
           cellular functions associated with ATP reduction in osteosarcoma cells
           treated with ethidium bromide

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      Abstract: Abstract Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA1 to LPA6) exhibits a variety of malignant properties in cancer cells. Intracellular ATP depletion leads to the development of necrosis and apoptosis. The present study aimed to evaluate the effects of LPA receptor-mediated signaling on the regulation of cancer cell functions associated with ATP reduction. Long-term ethidium bromide (EtBr) treated (MG63-EtBr) cells were established from osteosarcoma MG-63 cells. The intracellular ATP levels of MG63-EtBr cells were significantly lower than that of MG-63 cells. LPAR2, LPAR3, LPAR4 and LPAR6 gene expressions were elevated in MG63-EtBr cells. The cell motile and invasive activities of MG63-EtBr cells were markedly higher than those of MG-63 cells. The cell motile activity of MG-63 cells was increased by LPA4 and LPA6 knockdowns. In cell survival assay, cells were treated with cisplatin (CDDP) every 24 h for 3 days. The cell survival to CDDP of MG63-EtBr cells was lower than that of MG-63 cells. LPA2 knockdown decreased the cell survival to CDDP of MG-63 cells. The cell survival to CDDP of MG-63 cells was inhibited by (2 S)-OMPT (LPA3 agonist). Moreover, the cell survival to CDDP of MG-63 cells was enhanced by LPA4 and LPA6 knockdowns. These results indicate that LPA signaling via LPA receptors is involved in the regulation of cellular functions associated with ATP reduction in MG-63 cells treated with EtBr.
      PubDate: 2022-03-08
      DOI: 10.1007/s10863-022-09933-8
       
  • HyfF subunit of hydrogenase 4 is crucial for regulating FOF1 dependent
           proton/potassium fluxes during fermentation of various concentrations of
           glucose

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      Abstract: Abstract Escherichia coli anaerobically ferment glucose and perform proton/potassium exchange at pH 7.5. The role of hyf (hydrogenase 4) subunits (HyfBDF) in sensing different concentrations of glucose (2 g L−1 or 8 g L−1) via regulating H+/K+ exchange was studied. HyfB, HyfD and HyfF part of a protein family of NADH-ubiquinone oxidoreductase ND2, ND4 and ND5 subunits is predicted to operate as proton pump. Specific growth rate was optimal in wild type and mutants grown on 2 g L−1 glucose reaching ~ 0.8 h−1. It was shown that in wild type cells proton but not potassium fluxes were stimulated ~ 1.7 fold reaching up to 1.95 mmol/min when cells were grown in the presence of 8 g L−1 glucose. Interestingly, cells grown on peptone only had similar proton/potassium fluxes as grown on 2 g L−1glucose. H+/K+ fluxes of the cells grown on 2 g L−1 but not 8 g L−1 glucose depend on externally added glucose concentration in the assays. DCCD-sensitive H+ fluxes were tripled and K+ fluxes doubled in wild type cells grown on 8 g L−1 glucose compared to 2 g L−1 when in the assays 2 g L−1glucose was added. Interestingly, in hyfF mutant when cells were grown on 2 g L−1glucose and in 2 g L−1 assays DCCD-sensitive fluxes were not determined compared to wild type while in hyfD mutant it was doubled reaching up to 0.657 mmol/min. In hyf mutants DCCD-sensitive K+ fluxes were stimulated in hyfD and hyfF mutants compared to wild type but depend on external glucose concentration. DCCD-sensitive H+/K+ ratio was equal to ~ 2 except hyfF mutant grown and assayed on 2 g L−1glucose while in 8 g L−1 conditions role of hyfB and hyfD is considered. Taken together it can be concluded that Hyd-4 subunits (HyfBDF) play key role in sensing glucose concentration for regulation of DCCD-sensitive H+/K+ fluxes for maintaining proton motive force generation.
      PubDate: 2022-02-02
      DOI: 10.1007/s10863-022-09930-x
       
 
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