|
|
- Modulation effects of pressing manipulation on local inflammatory
responses and ERK/NF-κB pathway in trigger point model rats-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To investigate the mechanism of trigger point deactivation induced by pressing manipulation in a rat model and to explore its potential regulation of the inflammatory response through the extracellular signal-regulated kinase (ERK)/nuclear factor-κB (NF-κB) pathway. Methods Fifty male Sprague-Dawley rats were randomly divided into a blank group, a model group, a pressing manipulation group, an ERK agonist group, and a pressing manipulation + ERK agonist group, with 10 rats in each group. Except for the blank group, rats in other groups were used to establish the trigger point rat model using the blunt blow combined with the eccentric exercise method. The pressing manipulation group underwent pressing manipulation treatment at the trigger points. The ERK agonist group received an injection of recombinant human epidermal growth factor via the tail vein. The pressing manipulation + ERK agonist group received interventions from both the pressing manipulation and ERK agonist groups. The pressure pain threshold (PPT) was measured by a mechanical pain threshold detector before and after the intervention. The histological changes were evaluated by hematoxylin-eosin staining after the intervention; the expression levels of ERK, phosphorylated ERK (p-ERK), NF-κB p65 (p65), phosphorylated NF-κB p65 (p-p65), and phosphorylated NF-κB inhibitor (p-IκB) were detected by immunoblotting; the levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α were detected by enzyme-linked immunosorbent assay. Results The PPT increased (P<0.05); the inflammatory cells disappeared; the ratios of p-ERK/ERK, p-p65/p65, and p-IκB/β-actin, also the levels of IL-1β, IL-6, and TNF-α all decreased in the pressing manipulation group after the intervention compared with the model group (P<0.05). The PPT decreased significantly (P<0.05), the inflammatory cell presence increased, and the ratios of p-ERK/ERK and p-p65/p65 were elevated (P<0.05); additionally, the levels of IL-6 and TNF-α were significantly higher in the pressing manipulation + ERK agonist group compared with the pressing manipulation group (P<0.05). The PPT was significantly lower (P<0.05), the inflammatory cell count was higher, the ratios of p-ERK/ERK and p-IκB/β-actin and the levels of IL-1β and TNF-α were significantly higher in the ERK agonist group compared with the pressing manipulation + ERK agonist group (P<0.05). Conclusion Pressing manipulation can effectively alleviate inflammation and pain in trigger point model rats, potentially by inhibiting the ERK/NF-κB signaling pathway.
PubDate: 2024-08-08
- Mechanism of moxibustion in treating chronic inflammatory visceral pain:
regulation of the p38 MAPK/ELK1 signaling pathway in the spinal cord-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To investigate the central mechanism of moxibustion in treating chronic inflammatory visceral pain (CIVP) and its analgesic effect from the perspective of the p38 mitogen-activated protein kinase (MAPK)/Ets-like transcription factor 1 (ELK1) signaling pathway in the spinal cord. Methods Clean-grade male Sprague-Dawley rats were randomly divided into a normal group, a model group, a herb-partitioned moxibustion (HPM) group, a sham-HPM group, a p38 MAPK inhibitor group, and a dimethyl sulfoxide (DMSO) group. CIVP rat models were prepared using an enema mixture of 2,4,6-trinitrobenzene sulfonic acid solution and 50% ethanol. The HPM group was treated with HPM; the sham-HPM group was treated the same as the HPM group, but the moxa cones were not ignited; rats in the p38 MAPK inhibitor group received L5-L6 intrathecal injection of p38 MAPK inhibitor (SB203580); rats in the DMSO group received L5-L6 intrathecal injection of 2% DMSO. Abdominal withdrawal reflex (AWR), mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL) were used to observe pain-related behaviors in each group. Hematoxylin-eosin staining was used to observe the morphological changes in rat colon tissue. Western blotting and real-time quantitative reverse-transcription polymerase chain reaction were used to detect the phosphorylated protein and mRNA expression of apoptosis signal-regulating kinase 1 (ASK1), MAPK kinase (MKK) 3/6, p38 MAPK, ELK1, and mitogen and stress-activated protein kinase 1 (MSK1) in the spinal cord. Results Compared with the normal group, CIVP rats had severe colonic inflammatory injuries, and the pathological injury scores increased significantly, along with increased AWR scores under different colorectal distension (CRD) stimulation pressures and decreased MWT and TWL; the mRNA and phosphorylated protein expression of p38 MAPK, ELK1, MSK1, ASK1, MKK3, and MKK6 all increased in the spinal cord (P<0.01). After HPM treatment, the colon injuries were repaired, and the pathological injury scores decreased; under different CRD stimulation pressures, the AWR scores decreased, and the MWT and TWL increased; the mRNA and phosphorylated protein expression of p38 MAPK, ELK1, ASK1, and MKK3 in the spinal cord also decreased, with statistically significant differences compared with the model group and the sham-HPM group (P<0.01). There were no significant differences in the above indicators between the HPM group and the p38 MAPK inhibitor group (P>0.05), and the same was true regarding the comparisons between the model group and the DMSO group. Conclusion HPM exerted analgesic effects via downregulating the mRNA and phosphorylated protein expression of ASK1, MKK3, p38 MAPK, and ELK1 in the spinal cord of CIVP rats. The inhibition of spinal p38 MAPK/ELK1 signaling pathway activation may be one of the mechanisms by which HPM relieves pain in CIVP.
PubDate: 2024-08-01
- Effects of moxibustion on CD4+ T-cell proliferation by G6PD-mediated
pentose phosphate pathway in adjuvant arthritis rats-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of moxibustion on the levels of glucose-6-phosphate dehydrogenase (G6PD) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the plasma and spleen and the CD4+ T-cell number in the spleen of rats with adjuvant arthritis, thus to explore the mechanism in rheumatoid arthritis (RA) treatment with moxibustion by regulating the CD4+ T-cell proliferation through G6PD-mediated pentose phosphate pathway. Methods Twenty-seven male Sprague-Dawley rats were randomly divided into a blank group, a model group, and a moxibustion group, with 9 rats in each group. Incomplete Freund’s adjuvant was used to induce inflammation in the model group and the moxibustion group. The blank group and the model group were not intervened. In the moxibustion group, suspended moxibustion was performed at bilateral Zusanli (ST36), Guanyuan (CV4), and Ashi points for 30 min, once a day for 24 times in total. Hematoxylin-eosin staining was used to evaluate the histopathological changes of rat synovial tissue; the swelling degree of the rat toes was observed by measuring the toe volume; G6PD and NADPH in the spleen and plasma were detected by Western blotting and enzyme-linked immunosorbent assay. Flow cytometry was used to detect the CD4+ T-cell number in the spleen. Results Compared with the blank group, the levels of G6PD and NADPH in the plasma and spleen and the CD4+ T-cell number in the spleen were significantly increased in the model group (P<0.01 or P<0.05). Compared with the model group, the NADPH level in the spleen and plasma and the CD4+ T-cell number in the spleen in the moxibustion group decreased significantly (P<0.05 or P<0.01), and the G6PD level in the plasma decreased significantly (P<0.05), but there was no significant difference in the G6PD level in the spleen (P>0.05). Conclusion Moxibustion can regulate immunity and improve joint synovial inflammation in RA. The mechanism may be that the G6PD-mediated pentose phosphate pathway reduces the production of metabolite NAPDH in CD4+ T cells, thereby inhibiting the proliferation of naive CD4+ T cells.
PubDate: 2024-08-01
- Effects of acupuncture medication combined on facial function and mimetic
muscle strength and motor conduction in patients with peripheral facial
palsy-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the improvement of facial weakness in patients with peripheral facial palsy treated with needling the muscle regions of meridians plus Da Qin Jiao Tang [decoction of Qin Jiao (Radix Gentianae Macrophyllae)], and the effects on facial paralysis injury function and mimetic muscle atrophy and motor conduction. Methods A total of 120 patients with peripheral facial palsy were randomized into a Chinese medication group and an acupuncture medication combined group by the random number table method, with 60 cases in each group. The Chinese medication group was treated with Da Qin Jiao Tang, and the acupuncture medication combined group was treated with needling the muscle regions of meridians based on oral Da Qin Jiao Tang. After treatment, the clinical efficacy was compared between the two groups. The changes in the facial symptom score, facial muscle thickness, facial muscle strength, facial nerve function, and facial disability index were observed. Results The total effective rate in the acupuncture medication combined group was 98.3%, which was higher than 85.0% in the Chinese medication group (P<0.05). Each facial symptom score in the acupuncture medication combined group was lower than that in the Chinese medication group (P<0.05). The muscle thickness of the depressor anguli oris, frontalis, and depressor labii inferioris in the acupuncture medication combined group was thicker than that in the Chinese medication group (P<0.05). The scores of physical function and social/behavioral function in the acupuncture medication combined group were lower than those in the Chinese medication group (P<0.05), while the scores of manual muscle test (MMT) and Sunnybrook facial nerve were higher than those in the Chinese medication group (P<0.05). The facial nerve motor latency of the upper lip quadrate muscle, frontalis, and orbicularis oculi in the acupuncture medication combined group was lower than that in the Chinese medication group (P<0.05), while the M-wave amplitude was higher than that in the Chinese medication group (P<0.05). Conclusion Needling the muscle regions of meridians plus Da Qin Jiao Tang can improve facial muscle strength, mimetic muscle atrophy, facial paralysis function injury, and facial nerve motor conduction in the treatment of peripheral facial palsy and enhance clinical efficacy.
PubDate: 2024-08-01
- Clinical study on different needling depths in treating knee
osteoarthritis based on high-frequency ultrasound evaluation-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the efficacy of different needling depths for knee osteoarthritis (KOA) and the effects on effusion, cartilage thickness, and synovium under ultrasound imaging. Methods A total of 90 patients with KOA were randomly divided into a conventional acupuncture group, a Western medication group, and a shallow acupuncture group, with 30 cases in each group. In the conventional acupuncture group, the needling depth was 15–30 mm, and the needle was twisted for a few moments after insertion so that a sore, numb, distending, and electrified sensation was obtained; in the shallow acupuncture group, the points were punctured perpendicularly by 2 mm, and no manipulation was performed after insertion. The same points were used in the two acupuncture groups, and the needles were retained for 20 min. The treatment was performed 3 times a week. The Western medication group was given glucosamine sulfate capsules for oral administration, 2 capsules once, 3 times a day. All three groups were treated for 8 weeks. The Western Ontario and McMaster Universities osteoarthritis index (WOMAC) and the visual analog scale (VAS) were scored before treatment, after 4 weeks and 8-week treatment, respectively; while the changes in knee cartilage, synovial membrane, and joint fluid were observed by knee ultrasound before and after 8-week treatment. The clinical efficacy was evaluated after 8-week treatment. Results After 8-week treatment, the effusion and the synovial thickness improved significantly in both the conventional and shallow acupuncture groups compared with that before treatment (P<0.01); the effusion improved in the Western medication group compared with that before treatment (P<0.05); the synovial thickness improved better in the conventional acupuncture group than in the Western medication group (P<0.05). There was no statistically significant difference in the cartilage thickness among the three groups compared with that before treatment (P<0.05). The WOMAC (pain, stiffness, and function) and VAS scores decreased in all three groups after 4- and 8-week treatment compared with those before treatment (P<0.05). After 4- and 8-week treatment, the pain scores in WOMAC of the conventional acupuncture group were lower than those of the Western medication group and the shallow acupuncture group (P<0.05), and the VAS scores of the conventional acupuncture group were lower than those of the shallow acupuncture group (P<0.05). After 8-week treatment, the stiffness and functional scores in WOMAC of the conventional acupuncture group were lower than those of the Western medication group (P<0.05). Conclusion Conventional acupuncture, oral Western medication, and shallow acupuncture all relieved knee pain and improved knee stiffness and functional movement. Among them, conventional acupuncture had the best efficacy in relieving knee pain and the best efficacy in improving joint function after 8-week treatment. Both conventional acupuncture and shallow acupuncture significantly reduced synovial thickness lesions in the knee; all three groups improved joint effusion and were more effective with conventional and shallow acupuncture.
PubDate: 2024-08-01
- Observation on the efficacy of Tuina combined with azithromycin in the
treatment of chronic cough after Mycoplasma pneumoniae infection in
children-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To investigate the clinical efficacy of Yun Pi Tiao Fei (spleen-strengthening and lung-regulating) Tuina (Chinese therapeutic massage) combined with azithromycin in the treatment of chronic cough due to Qi deficiency of lung and spleen after Mycoplasma pneumoniae infection in children. Methods A total of 100 children with chronic cough due to Qi deficiency of lung and spleen after Mycoplasma pneumoniae infection were enrolled and divided into a control group and an observation group according to the random number table method, with 50 cases in each group. The control group was treated with oral azithromycin, and the observation group was treated with Yun Pi Tiao Fei Tuina on the basis of the treatment in the control group, with each session lasting about 35 min, and the treatment was carried out once a day. After 14 d consecutive treatments, the cough score, traditional Chinese medicine symptom score, clinical symptoms or signs disappearance time, serum inflammatory factors [tumor necrosis factor (TNF)-α, C-reactive protein (CRP), interleukin (IL)-6], eosinophilic cationic protein (ECP), eosinophils (EOS), and immunoglobulin (Ig) E levels were observed, and the clinical efficacy and safety of the two groups were compared. Results The total effective rate was 96.0% in the observation group, which was higher than 78.0% in the control group (P<0.05). After treatment, the daytime and nighttime cough scores and total cough score in the two groups were reduced (P<0.05), and the scores in the observation group were lower than those in the control group (P<0.05). After treatment, the scores of repeated coughing, weak coughing, tiredness and weakness, white sputum with diluted texture, and formless stools in the two groups were decreased (P<0.05), and the scores in the observation group were lower than those in the control group (P<0.05). After treatment, the levels of TNF-α, CRP, IL-6, ECP, EOS, and IgE in the two groups were decreased (P<0.05), and the levels in the observation group were lower than those in the control group (P<0.05). The disappearance time of cough, coarse breath sounds, and airway hyperresponsiveness came earlier in the observation group than in the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion Yun Pi Tiao Fei Tuina combined with azithromycin has a significant effect in the treatment of chronic cough due to Qi deficiency of lung and spleen after Mycoplasma pneumoniae infection in children. It can inhibit infection, shorten the course of disease, and promote recovery, causing fewer adverse reactions.
PubDate: 2024-08-01
- Effects of pecking moxibustion on pattern characteristics and synovial
cell ultrastructure of rats with rheumatoid arthritis due to damp heat
affecting bones/joints-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effect of pecking moxibustion on the pattern characteristics of redness, swelling, heat, and pain in the affected joints, also the synovial cell ultrastructure in rats with rheumatoid arthritis (RA) due to damp heat affecting bones/joints, and to explore the anti-inflammatory mechanism of pecking moxibustion in treating the early stage of RA. Methods Eighteen rats were randomly selected from 78 female ones as the blank group, and all the other rats were subjected to preparing the “differentiation of disease and pattern” RA model due to damp heat affecting bones/joints by using the method of “collagen-induced arthritis plus windy, damp, and hot environment stimulation”. Fifty-four rats with successful modeling were randomly divided into a model group, a drug group, and a pecking moxibustion group, with 18 rats in each group. Rats in the drug group were given methotrexate at a dose of 1 mg/(kg·bw) on the 1st, 8th, and 15th days. Rats in the pecking moxibustion group were treated with pecking moxibustion at Quchi (LI11), Dazhui (GV14), and Ashi points, and each point was treated with moxibustion for 15 min every day and a total of 3 courses of treatment, with 6 d as a course of treatment. After treatment, the capillary permeability, joint swelling, joint surface temperature, and plantar thermal pain threshold of the diseased joints in rats were observed, and the ultrastructural changes of synovial cells were observed by transmission electron microscopy. Results The local swelling, surface temperature, and Evans blue (EB) leakage volume were significantly higher (P<0.05), the thermal pain threshold was significantly lower (P<0.05), and the synovial cell ultrastructure was obviously damaged in the affected joints in the model group compared with the blank group. The swelling degree, surface temperature, and EB leakage volume were significantly reduced (P<0.05), the thermal pain threshold was significantly increased (P<0.05), and the ultrastructural abnormalities of synovial cells were significantly improved in the diseased joints in the drug group and the pecking moxibustion group compared with the model group. The thermal pain threshold of rats in the pecking moxibustion group was significantly improved compared with the drug group (P<0.05). Conclusion Pecking moxibustion obviously improves the pattern characteristics of local redness, swelling, heat, and pain in the diseased joints of rats with RA due to damp heat affecting bones/joints and effectively repairs the ultrastructure of the damaged synovium. It suggests that the pecking moxibustion intervention has a significant anti-inflammatory effect on early RA.
PubDate: 2024-08-01
- Effects of point application and ear point pressing therapy plus nasal
spray on inflammatory cells and immune function in patients with allergic
rhinitis-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of point application, ear point pressing therapy, plus mometasone furoate nasal spray on inflammatory cells and immune function in patients with allergic rhinitis (AR). Methods Fifty AR patients who received point application, ear point pressing therapy, and mometasone furoate nasal spray for treatment were recruited as an observation group. Simultaneously, another 50 AR patients who only used the same nasal spray were enrolled as a control group. The clinical efficacy was compared between the two groups, and the inflammatory cell count and immune function changes were observed after treatment. Results The observation group had a higher total effective rate than the control group (P<0.05). After treatment, significant decreases were noticed in the total nasal symptom score, eosinophils count in nasal secretions, and serum immunoglobulin E (IgE) level in both groups (P<0.05), and the observation group was significantly lower than the control group (P<0.05). Conclusion Combining point application, ear point pressing therapy, and mometasone furoate nasal spray can produce satisfactory treatment results for AR. This method is safe and can reduce inflammatory responses and improve immune function.
PubDate: 2024-08-01
- Clinical observation of warming needle therapy combined with Chinese
medication in treating post-stroke cognitive impairment due to obstruction
of wind-phlegm-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of warming needle therapy combined with the modified Hua Tan Tong Luo Tang (decoction for dissipating phlegm and unblocking collaterals) on post-stroke cognitive impairment due to obstruction of wind-phlegm. Methods A total of 78 patients who met the inclusion criteria were randomly assigned to an observation group and a control group, with 39 patients in each group. Both groups received conventional treatment. The control group was additionally treated with the modified Hua Tan Tong Luo Tang, while the observation group received warming needle therapy in addition to the treatment given to the control group. Scores on the mini-mental state examination (MMSE), Montreal cognitive assessment (MoCA), and activities of daily living (ADL) were assessed before and after treatment. Adverse reactions and clinical efficacy were analyzed and compared between the two groups after treatment. Results The total effective rate was 82.1% in the observation group and 61.5% in the control group, showing statistical significance between the two groups (P<0.05). After treatment, the MMSE, MoCA, and ADL scores of both groups were significantly higher than those before treatment within each group (P<0.05). Notably, the scores in the observation group were higher than those in the control group (P<0.05). No adverse reactions occurred in either group during the treatment period. Conclusion Based on the conventional treatment, compared to modified Hua Tan Tong Luo Tang alone, warming needle therapy combined with the modified Hua Tan Tong Luo Tang showed superior clinical efficacy in treating post-stroke cognitive impairment due to obstruction of wind-phlegm, and this combined therapy also showed high safety.
PubDate: 2024-08-01
- Clinical study on analgesic effect of acupuncture combined with point
injection in patients with discogenic low back pain-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effect of acupuncture combined with vitamin B12 point injection on pain severity in patients with discogenic low back pain and to analyze its potential mechanisms. Methods A total of 96 patients with discogenic low back pain were randomly divided into two groups. The control group received acupuncture treatment, while the combined group received vitamin B12 point injection in addition to the identical acupuncture treatment in the control group. Both groups were treated for 2 weeks. The visual analog scale (VAS) and Oswestry disability index (ODI) scores were compared before treatment, after 1 week of treatment, and after 2 weeks of treatment. The levels of such serum inflammatory factors as tumor necrosis factor (TNF)-α and interleukin (IL)-6, serum beta-endorphin (β-EP), and prostaglandin (PG) E2 were compared before and after treatment. Adverse reactions and clinical efficacy were compared between the two groups after treatment. Results The total effective rate in the combined group was higher than that in the control group (P<0.05). The VAS and ODI scores in the combined group after 1 week and 2 weeks of treatment were lower than those in the control group at the same time point (P<0.05). The levels of TNF-α, IL-6, and PGE2 in the combined group were lower than those in the control group after treatment (P<0.05), while the level of β-EP was higher than that in the control group (P<0.05). There was no statistical difference in the overall incidence of adverse reactions between the two groups (P<0.05). Conclusion Acupuncture combined with vitamin B12 point injection can alleviate pain and promote functional recovery in patients with discogenic low back pain; reducing the levels of TNF-α, IL-6, and PGE2 and increasing the level of β-EP may be part of the mechanism of the therapy.
PubDate: 2024-08-01
- Effects of interactive Tuina on spatiotemporal gait parameters in children
with spastic cerebral palsy-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To discuss the effects of walking function-guided interactive Tuina (Chinese therapeutic massage) on spatiotemporal gait parameters in children with spastic cerebral palsy (CP). Methods Fifty children with spastic diplegic CP were divided into an observation group and a control group following a paired design and random number principle, with 25 cases in each group. The observation group was treated with the walking function-guided interactive Tuina, and the control group was offered the “six methods of spine and back” Tuina, 20 min for each session and 5 sessions weekly for 3 consecutive months. The children’s changes were assessed using the percentage of dimension E (walking, running, and jumping) of the gross motor function measure-88 (GMFM-88) and three-dimensional gait analysis. Results After treatment, the percentage of GMFM-88 dimension of dimension E (walking, running, and jumping) changed significantly in both groups (P<0.05), and the between-group difference was also statistically significant (P<0.05). After treatment, the step length, walking speed, and step frequency changed markedly in the observation group (P<0.05) and were significantly different from those in the control group (P<0.05). After the intervention, the stance phase and double support extended, and the swing phase became shorter in the observation group (P<0.05); the between-group differences were statistically significant (P<0.05). The maximal knee flexion angle and maximal posterior pelvic tilt angle decreased After treatment in the observation group, and the maximal anterior pelvic tilt angle increased, all showing statistical significance (P<0.05); the between-group differences were statistically significant (P<0.05). Conclusion Compared with the passive Tuina manipulations, the “six methods of the spine and back”, walking function-guided interactive Tuina has its advantage in improving walking function in children with spastic CP, manifesting as better lower-limb force line and walking efficiency.
PubDate: 2024-08-01
- Acupuncture treatment for central post-stroke pain: a systematic review
and meta-analysis-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To evaluate the efficacy and safety of acupuncture in the treatment of central post-stroke pain (CPSP). Methods Randomized controlled trials of acupuncture treatment for CPSP in PubMed, Excerpta Medica Database (EMBASE), Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform (Wanfang), Chongqing VIP Database (VIP), and China Biology Medicine Disc (CBM) were retrieved by computer. The retrieval time was from each database’s inception to July 2023. Meta-analysis was performed using RevMan 5.3 software; GRADEprofiler 3.6.1 software was used to evaluate the quality of evidence. Dichotomous variables were analyzed by the risk ratio (RR). Continuous data were analyzed by mean difference (MD) with a confidence interval (CI) of 95%. Results A total of 14 studies were included, comprising a total of 1 045 patients. The findings of the meta-analysis showed that compared with Western medication in treating CPSP, the acupuncture treatment had a higher clinical effective rate [RR=1.09, 95%CI (1.01, 1.19), Z=2.08, P<0.05], a lower visual analog scale (VAS) score [MD=−0.75, 95%CI (−1.18, −0.32), Z=3.41, P<0.001], a lower pain rating index (PRI) score [MD=−1.72, 95%CI (−2.76, −0.68), Z=3.24, P<0.05], a higher plasma β-endorphin (β-EP) level [MD=5.81, 95%CI (3.00, 8.62), Z=4.05, P<0.001], and a lower adverse reaction rate [RR=0.05, 95%CI (0.01, 0.18), Z=4.35, P<0.001]. There was no statistical difference in the present pain intensity (PPI) score between the two treatments [MD=−0.26, 95%CI (−0.54, 0.02), Z=1.79, P>0.05]. Compared with Western medication in treating CPSP, acupuncture plus Western medication had a higher clinical effective rate [RR=1.18, 95%CI (1.05, 1.34), Z=2.75, P<0.05], a lower VAS score [MD=−1.04, 95%CI (−1.26, −0.82), Z=9.25, P<0.001], and a lower Pittsburgh sleep quality index (PSQI) score [MD=−2.67, 95%CI (−4.80, −0.54), Z=2.46, P<0.05]. The results of the evidence quality grade evaluation showed that there was no moderate- or high-quality evidence for acupuncture or acupuncture plus Western medication compared with Western medication in the treatment of CPSP. Conclusion Acupuncture has certain therapeutic advantages over Western medication in the treatment of CPSP. It can effectively relieve pain and improve sleep, with fewer adverse reactions and better safety. However, high-quality randomized controlled trials are still needed for further study and verification.
PubDate: 2024-08-01
- Effect mechanism investigation of herb-partitioned moxibustion on
relieving colon inflammation in Crohn disease rats based on neutrophil
extracellular traps-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To explore the mechanism of herb-partitioned moxibustion in relieving rat intestinal inflammation by focusing on the neutrophil extracellular traps (NETs) in Crohn disease (CD) development. Methods Rats were randomly divided into a normal group, a model group, a herb-partitioned moxibustion group, and a mesalazine group. The CD rat model was prepared with 2,4,6-trinitrobenzene sulfonic acid except for rats in the normal group. Rats in the normal group and model group did not receive any treatment but had the same fixation as the other groups. Rats in the herb-partitioned moxibustion group received herb-partitioned moxibustion at Qihai (CV6) and bilateral Tianshu (ST25). Rats in the mesalazine group received intragastric administration of mesalazine enteric-coated tablets. The general situation of rats in each group was recorded, and the histopathological changes in the colon were observed and scored by hematoxylin-eosin staining. The serum concentrations of NETs DNA (NETs-DNA), neutrophil elastase (NE)-DNA, and myeloperoxidase (MPO)-DNA were detected by ABC enzyme-linked immunosorbent assay, and the citrullinated histone 3 (citH3), MPO, and NE protein and mRNA expression levels in rat colon tissue were observed by immunofluorescence and real-time quantitative polymerase chain reaction. Results Compared with the normal group, the mucosal ulcer reached the muscularis, the epithelium was incomplete, the goblet cells decreased obviously with significant inflammatory cell infiltration in the colon; the colonic mucosa damage index (CMDI) score increased significantly (P<0.01); the serum NETs-DNA, NE-DNA, and MPO-DNA concentrations increased (P<0.05); the NE, citH3, and MPO protein and mRNA expression in the colonic tissues increased significantly in the model group (P<0.01 or P<0.05). Compared with the model group, the mucosal epithelium in the herb-partitioned moxibustion group and the mesalazine group was repaired and the goblet cells increased with a few infiltrating inflammatory cells in the colon; the CMDI score decreased (P<0.01); the serum NETs-DNA, NE-DNA, and MPO-DNA concentrations decreased (P<0.05); the NE, citH3, and MPO protein and mRNA expression in the colonic tissues was down-regulated (P<0.01 or P<0.05). Conclusion Herb-partitioned moxibustion reduced the serum NETs complex and inhibited the protein and mRNA expression of NETs complex in the colon tissue, which may be one mechanism of herb-partitioned moxibustion in relieving colon mucosal inflammation in CD.
PubDate: 2024-06-01
- Effects of preventative moxibustion at Shenque (CV8) and Guanyuan (CV4) on
BDNF-TrkB pathway in the spinal dorsal horn and hypothalamus in rats with
dysmenorrhea-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of preventative moxibustion on analgesia, substance P (SP), prostaglandin (PG) F2α and PGE2 in rats with dysmenorrhea due to cold-dampness stagnation, and to explore the analgesic mechanism. Methods Sixty-four female Wistar non-pregnant rats were randomly divided into a blank group, a model group, a Western medicine group, and a preventative moxibustion group, with 16 rats in each group. Eight qualified diestrus rats were selected from each group. Except for the blank group, the other three groups established models of dysmenorrhea due to cold-dampness stagnation using an ice water bath combined with estradiol benzoate and oxytocin. On the 8th day after modeling, the preventative moxibustion group was treated with gentle moxibustion at Shenque (CV8) and Guanyuan (CV4), and the Western medicine group was given ibuprofen solution for 4 consecutive days. On the 11th day, the intervention groups (i.e. the Western medicine group and the preventative moxibustion group) were treated once again after being injected with oxytocin. The writhing score and the pain threshold of rats were determined; the serum levels of brain-derived neurotrophic factor (BDNF), SP, PGF2α, and PGE2 were measured; the mRNA and protein expression levels of BDNF and its receptor tropomyosin receptor kinase B (TrkB) in the spinal dorsal horn and hypothalamus were detected. Results Compared with the blank group, the writhing score increased (P<0.01), the pain threshold decreased (P<0.01), the serum levels of BDNF, SP, and PGF2α increased (P<0.01), while the PGE2 decreased (P<0.01); the protein and mRNA expression levels of BDNF and TrkB in the spinal dorsal horn and hypothalamus increased (P<0.01) in the model group. Compared with the model group, the writhing score decreased, the pain threshold increased, the serum BDNF, SP, and PGF2α levels decreased significantly, the serum PGE2 level increased, and the protein and mRNA expression levels of BDNF and TrkB in the spinal dorsal horn and hypothalamus decreased significantly in the preventative moxibustion group and the Western medicine group, while the inter-group differences were significant (P<0.01). Compared with the Western medicine group, the writhing score decreased, the pain threshold increased, the serum BDNF, SP, and PGF2α, levels decreased, the serum PGE2 level increased, and the protein and mRNA expression levels of BDNF and TrkB in the spinal dorsal horn and hypothalamus in the preventative moxibustion group decreased significantly, while the inter-group differences were significant (P<0.05 or P<0.01). Conclusion Preventative moxibustion at Shenque (CV8) and Guanyuan (CV4) can improve the pain sensitization state of rats with dysmenorrhea due to cold-dampness stagnation, down-regulate the mRNA and protein expression levels of BDNF and TrkB in the spinal dorsal horn and hypothalamus; regulation of the serum SP, PGF2α, and PGE2 levels may be part of the mechanism.
PubDate: 2024-06-01
- Effects of acupuncture and moxibustion on UHRF1 and DNMT1 in ectopic
endometrium of rats with endometriosis-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of acupuncture and moxibustion on ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) and DNA methyltransferase 1 (DNMT1) in ectopic endometrium of rats with endometriosis (EMS). Methods Forty Sprague-Dawley rats were randomly divided into a sham operation group with 10 rats and a model-building group with 30 rats according to body mass. EMS rat models were established in the model-building group and then were divided into a model group, an acupuncture and moxibustion group, and a progesterone group, with 10 rats in each group. All rats were fixed by a fixator. The sham operation group and the model group were given normal saline by gavage. The acupuncture and moxibustion group received acupuncture at Xuehai (SP10) and Sanyinjiao (SP6), moxibustion at Guanyuan (CV4), and gavage of normal saline. The progesterone group was given the mixed liquid made of dydrogesterone and normal saline by gavage. After 28 d of treatments, the three diameters (length, width, and height) of EMS rats’ ectopic cysts were measured, the cyst volumes were calculated, the volumes before intervention were subtracted, and the difference values were used to evaluate the growth of ectopic cysts. UHRF1 and DNMT1 mRNA and protein levels in normal endometrium, eutopic endometrium, and ectopic endometrium were detected by real-time polymerase chain reaction and immunohistochemistry. Results There was no significant difference in the ectopic cyst volume difference between the acupuncture and moxibustion group and the progesterone group (P>0.05), but they were smaller than that of the model group (P<0.05). The levels of UHRF1 and DNMT1 mRNA and protein in the ectopic endometrium of the model group were lower than those in the normal endometrium (P<0.05). The levels of DNMT1 mRNA and UHRF1 protein in the eutopic endometrium of the model group were lower than those in the normal endometrium (P<0.05). The levels of UHRF1 mRNA and protein and the level of DNMT1 protein in the ectopic endometrium of the acupuncture and moxibustion group were higher than those in the model group (P<0.05), and the level of UHRF1 mRNA was higher than that in the progesterone group (P<0.05). The level of DNMT1 mRNA in the eutopic endometrium of the acupuncture and moxibustion group was higher than that in the model group (P<0.05). The levels of UHRF1 and DNMT1 mRNA and protein in the acupuncture and moxibustion group were insignificantly different from those in the normal endometrium (P>0.05). Conclusion Acupuncture and moxibustion may up-regulate the levels of UHRF1 mRNA and UHRF1 and DNMT1 proteins in the ectopic endometrium to the normal level so as to reduce the volume of ectopic cysts and cure endometriosis in rats.
PubDate: 2024-06-01
- Herb-insulated moxibustion on the Governor Vessel in dog days of summer
for postpartum pantalgia: a randomized controlled trial-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the clinical efficacy of herb-insulated moxibustion on the Governor Vessel in dog days of summer for postpartum pantalgia. Methods A total of 64 patients with postpartum pantalgia were randomized into an observation group and a control group, with 32 cases in each group. Both groups were treated with herb-insulated moxibustion on the Governor Vessel. The control group was treated in non-dog days, and the observation group was treated in dog days of summer. Both groups were treated with moxibustion once every 3 d, 3 times as one course of treatment, for a total of 3 courses. The range and degree of pain were evaluated by the 45-region body surface area score and visual analog scale (VAS) score before treatment, after treatment, 1 month after treatment, and around the Winter Solstice. The revised fibromyalgia impact questionnaire (FIQR) was used to evaluate the quality of life of the patients. The clinical efficacy was compared between the two groups after treatment. Results During the trial, there were 2 dropout cases in the observation group, and 2 cases were eliminated from the control group. After treatment, the total effective rate in the observation group was 93.3%, which was higher than 76.6% in the control group (P<0.05). Compared with before treatment, the number of pain sites and pain areas of the patients in both groups decreased after treatment, 1 month after treatment, and around the Winter Solstice (P<0.05), and the FIQR score decreased (P<0.05). The score of each item in the observation group was lower than that in the control group (P<0.05). Compared with before treatment, the VAS score in the observation group decreased after treatment, 1 month after treatment, and around the Winter Solstice (P<0.01), and was lower than that in the control group at the same time point (P<0.05). Conclusion Herb-insulated moxibustion on the Governor Vessel can improve the range and degree of pain in patients with postpartum pantalgia and improve their quality of life. The total efficacy of the moxibustion performed in dog days is superior to that in non-dog days, and the effect lasts longer.
PubDate: 2024-06-01
- Clinical study of Yin-Yang balancing acupuncture plus Tuina in the
treatment of post-stroke depression-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the clinical efficacy of Yin-Yang balancing acupuncture plus Tuina (Chinese therapeutic massage) for post-stroke depression (PSD). Methods A total of 72 PSD patients were randomized into an acupuncture-Tuina group and a Western medicine group using the random number table method, with 36 cases in each group. The Western medicine group was treated with fluoxetine hydrochloride capsules. The acupuncture-Tuina group was treated with Yin-Yang balancing acupuncture plus Tuina therapy. Both groups were treated for 4 weeks. After treatment, the clinical efficacy was compared, and the changes in Hamilton depression scale (HAMD) score, National Institute of Health stroke scale (NIHSS) score, serum neurotransmitter levels, and electroencephalogram (EEG) were observed. Results The total effective rate of the Western medicine group and the acupuncture-Tuina group was 83.3% and 94.4%, respectively. There was a significant difference in the total effective rate between the two groups (P<0.05). After treatment, the serum levels of 5-hydroxytryptamine (5-HT), norepinephrine (NE), and dopamine (DA) were higher than those in the same group before treatment, and the intra-group differences were all statistically significant (P<0.05). The serum levels of 5-HT, NE, and DA in the acupuncture-Tuina group were higher than those in the Western medicine group, and the differences between the groups were statistically significant (P<0.05). After treatment, the scores of HAMD and NIHSS in both groups decreased, and the intra-group differences were statistically significant (P<0.05). The scores in the acupuncture-Tuina group were lower than those in the Western medicine group, and the differences between the two groups were all statistically significant (P<0.05). After treatment, the EEG diffuse wave [(delta + theta)/(alpha + beta)] ratio (DTABR) and delta/beta ratio (DBR) in both groups decreased, and the intra-group differences were statistically significant (P<0.05). The ratios in the acupuncture-Tuina group were lower than those in the Western medicine group, and the differences between the two groups were statistically significant (P<0.05). Conclusion Yin-Yang balancing acupuncture plus Tuina therapy has a better efficacy than oral fluoxetine hydrochloride capsules in the treatment of PSD, which can increase the levels of serum 5-HT, DA, and NE, and regulate brain waves.
PubDate: 2024-06-01
- Electroacupuncture combined with Western medication improves lower-limb
motor function and blood circulation in patients with cerebral
infarction-induced hemiplegia in acute stage: a clinical study-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the effects of electroacupuncture (EA) combined with Western medication on lower-limb motor function and blood circulation in patients with cerebral infarction-induced hemiplegia in the acute stage. Methods One hundred eligible patients with acute ischemic stroke accompanied by lower-limb motor dysfunction were allocated to an observation group and a control group using the random number table method, with 50 cases in each group. The control group received routine Western medications for treatment, and the observation group received additional EA intervention. After 2-week and 4-week treatments, the improvement of lower-limb motor function was assessed using the Fugl-Meyer assessment scale for lower extremity (FMA-LE), and changes in the peak blood flow velocities of the posterior tibial (PT) and dorsalis pedis (DP) arteries on the affected side were detected using Doppler. Results Three cases dropped out during the study, so there were 48 cases in the observation group and 49 in the control group collected for statistical analysis. The FMA-LE score and the peak blood flow velocities of PT and DP arteries increased after 2-week and 4-week treatments in both groups compared with the pre-treatment baseline (P<0.05). After 2-week treatments, the FMA-LE score and the peak blood flow velocities of PT and DP arteries showed no significant differences between the two groups (P>0.05). After 4-week treatments, compared with the control group, the FMA-LE score was higher (P<0.05), and the peak blood flow velocities of PT and DP arteries on the affected side were larger in the observation group (P<0.05). Conclusion EA combined with Western medication can significantly improve the motor function and blood flow velocity of the affected lower limb in patients with acute cerebral infarction accompanied by hemiplegia.
PubDate: 2024-06-01
- Inhibiting effects of manual acupuncture on bladder cell apoptosis in rats
with diabetic neurogenic bladder-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To observe the inhibiting effects of manual acupuncture (MA) on bladder cell apoptosis in rats with diabetic neurogenic bladder (DNB) based on the protein and mRNA expression of B-cell lymphoma-leukemia (Bcl)-2, Bcl-2-associated X (Bax) protein, caspase-3, and the protein expression of α-smooth muscle actin (α-SMA), transforming growth factor (TGF)-β in the bladder tissue. Methods A DNB rat model was established via intraperitoneal injection of streptozotocin (STZ). The rats were randomly divided into a control group, a model group, and an MA group, with 10 rats in each group. For the MA group, MA was applied after modeling. The body mass, fasting blood glucose (FBG), bladder wet weight, and bladder histomorphology were observed. Protein and mRNA expression levels of Bcl-2, Bax, and caspase-3 and the protein expression of α-SMA and TGF-β in the bladder tissue were determined. The apoptotic index of bladder cells was also evaluated. Results After STZ injection, compared with the control group, the model group and the MA group both showed higher FBG from week 3 and lower body mass from week 9 (P<0.05), and had a larger bladder wet weight (P<0.05). Compared with the model group, the MA group showed a smaller bladder wet weight (P<0.05). The histopathological evaluation indicated that MA improved muscle fiber alignment and detrusor cell compensatory hypertrophy in the bladder tissue. In addition, compared with the control group, the apoptotic index increased significantly in the model group and the MA group (P<0.05); the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-β in the bladder tissue in the model group and the MA group increased significantly (P<0.05), while the protein and mRNA expression levels of Bcl-2 and the protein expression level of α-SMA in the bladder tissue decreased significantly (P<0.05). Compared with the model group, the apoptotic index of the MA group decreased significantly (P<0.05); the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-β in the bladder tissue decreased significantly (P<0.05), while the protein and mRNA expression levels of Bcl-2 and the protein expression level of α-SMA in the bladder tissue increased significantly (P<0.05). Conclusion MA can protect the bladder by inhibiting the excessive apoptosis of bladder cells, which may be related to the down-regulation of Bax and caspase-3 proteins and mRNAs and TGF-β protein expression, and the up-regulation of Bcl-2 protein and mRNA and α-SMA protein expression.
PubDate: 2024-06-01
- Study of the clinical distinctions of acupuncture-moxibustion treatment of
acute gouty arthritis based on complex networks-
Free pre-print version: Loading...
Rate this result:
What is this?
Please help us test our new pre-print finding feature by giving the pre-print link a rating.
A 5 star rating indicates the linked pre-print has the exact same content as the published article.
Abstract: Objective To discuss the point-selection and point-grouping patterns and therapeutic application features in acupuncture-moxibustion treatment of acute gouty arthritis (AGA) based on complex networks and to provide references for treating AGA with acupuncture-moxibustion therapy. Methods Articles related to acupuncture-moxibustion treatment of AGA were searched across the China National Knowledge Infrastructure (CNKI), Chongqing VIP Database (CQVIP), Wanfang Data Knowledge Service Platform (Wanfang), Chinese Biomedical Literature Service System (SinoMed), PubMed, Web of Science (WOS), and Excerpta Medica Database (EMBASE) from their inception till March 31, 2023. An acupuncture-moxibustion prescription database was established after the articles were screened according to the inclusion and exclusion criteria. The association rule and complex network analyses of points were conducted using SPSS Modeler 18.1 and Gephi 0.9.7. Results A total of 145 articles were collected, contributing 382 pieces of acupuncture-moxibustion prescriptions involving 104 points with a total frequency of 1 288. Ashi points contributed the highest frequency. The Spleen Meridian of Foot-Taiyin and the Stomach Meridian of Foot-Yangming were more commonly selected. Filiform-needle acupuncture and bloodletting therapy were more frequently used. The association rule analysis revealed that the highest degree of support belonged to “Ashi point-Zusanli (ST36)” and “Sanyinjiao (SP6)-Zusanli (ST36)”, which reflected the rules of point combination of distal and proximal areas and point combination of the coupled meridians. The complex network analysis of the major points discovered a core point prescription mainly consisting of Ahi point, Zusanli (ST36), Sanyinjiao (SP6), Yinlingquan (SP9), and Taichong (LR3). Pattern differentiation and region differentiation were used in selecting adjunct points, stressing the improvements of patterns and joint-related symptoms. Conclusion Acupuncture-moxibustion treatment of AGA follows the principle of combining major points with adjunct points selected based on pattern or region differentiation; the selection of major points focuses on regulating the deficient Zang-Fu organs, and the selection of adjunct points emphasizes improving patterns and symptoms. The specificity of therapeutic effects is also stressed.
PubDate: 2024-06-01
|
Hybrid journal (It can contain Open Access articles)
