|
|
- Assessment of the Risk of Developing Cardiovascular Disease in Prediabetic
and Diabetic Wistar Rats with Insulinaemia, in a Selected Model
Authors: B. M. Aleme, B. A. Amadi
Pages: 1 - 11
Abstract: Introduction: This examination assessed the risk of developing cardiovascular disease in pre-diabetic and diabetes with insulinaemia in Wistar rats using the HOMA-IR model. The assessment solidified the role of Wistar rats in the evaluation model and took the blood analytes to screen for diabetes. Methodology: 84 male Wistar rats measuring 175g were isolated into fourteen groups of six animals each and dealt with high fat eating standard and acknowledged with dexamethasone which achieved diabetes. Group 1 animals were given feed and water. Group 2 animals got no treatment. Groups 3-5 were coordinated Glimepiride and Metformin, and Cinnamon independently while Groups 6-14 were administered 100mg/kg, 300mg/kg, and 500mg/kg of Ginger, Aloe Vera, and mix of Ginger and Aloe Vera exclusively. The concentrates were administered orally. Every illustration of blood serum and plasma was explored using Randox and Accubind packs and an autoanalyser to test for various biochemical and hematological limits. Results: The overall results revealed a colossal differentiation (p≤0.05) in the limits except for that of Na+. The 500mg/kg body weight part of the concentrate was ideal while there was much basic development in the HOMA-IR with potential gains of 0.94±0.04, 2.28±0.17 and 3.25±0.44 for the three sets. HOMA-IR is enticing over other models, as reported by other investigators. This assessment revealed that HOMA-IR works best in the management of diabetes in connection with various models. Contributions to Knowledge: HOMA-IR works best at predicting and managing DM. Administration of varying doses of the extracts to the Wistar rats helped in the control by ameliorating the effect of diabetes as seen in the return of some diabetic markers assayed. This research used Wistar rats in the evaluation of the HOMA-IR model. This research compared the blood analytes of non-diabetics, pre-diabetics, and diabetics in order to monitor the onset of diabetes and proffer possible solutions to enhance early detection and manage diabetes.
PubDate: 2022-05-07
DOI: 10.9734/jocamr/2022/v18i130341
- Ameliorating Effect of Methanol Extract of Finger Root Bark (Uvaria
chamae) on some Selected Biochemical Parameters of CdCl2 Induced Toxicity
in Wistar Rats
Authors: George C. Njoku, Prisca C. Aririguzo, Emmanuel K. Amanze, Isabel C. Nwagu, Chizurum P. Christian, Agbagwara C. Queendarlyn
Pages: 12 - 22
Abstract: Background: High cadmium-induced injuries have necessitated the search for potent and cost-effective herbal management models. Hence, this study aimed at evaluating the ameliorating effect of methanol extract of finger root bark (Uvaria chamae) on some selected biochemical parameters of cadmium-induced toxicity in Wistar rats. Methods: Thirty (30) Male rats weighing 84- 183 grams were obtained and kept in the animal house to acclimatize for eight (8) days prior to the experiment. The rats were grouped into five groups containing six rats each. Group 1 served as normal control; group 2 served as the negative control treated with 10mg/kg bw CdCl2; group 3 contained rats fed with 500mg/kg bw plant extract; Group 4-5 contained cadmium-intoxicated rats treated with Uvaria chamae (250mg/kg body weight and 250mg/kg body weight) respectively for 13 days. Thereafter, the rats were sacrificed, and blood samples were collected through the ocular puncture for biochemical analysis. Activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), as well as lipid profiles were assessed. Tissue antioxidant levels of reduced glutathione (GSH), malondialdehyde (MDA), and activities of antioxidant enzymes such as superoxide dismutase (SOD) were also determined. Result: CdCl2 caused a significant derangement in lipid profile, increasing serum triglyceride, total cholesterol and low-density lipoprotein (LDL) while high-density lipoprotein (HDL) level was diminished. Liver biomarkers (ALT, AST, ALP) were also significantly elevated in the serum relative to the control animals following exposure to CdCl2. Activities of antioxidant enzymes in the serum were markedly inhibited by CdCl2 exposure. Treatment with Uvaria chamae extract caused a dose-dependent restoration of all biochemical parameters determined. Conclusion: The findings show the potential usefulness of Uvaria chamae extract in managing certain diseases.
PubDate: 2022-05-09
DOI: 10.9734/jocamr/2022/v18i130342
- Phytochemical Analysis of Some Medicinal Plants Used for the Management of
Reproductive Health Care Problems in the West Region in Cameroon
Authors: Roger Tsobou, Martin Lekeufack, Lucy Ambang Agyingi, Mariette Anouma’a, Bertine Tiokeng, Patrick Sonkoue Njimeli, Maurice Ducret Awouafack, Pierre-Marie Mapongmetsem, Van Damme Patrick
Pages: 23 - 35
Abstract: Reproductive diseases are conditions that negatively affects human reproductive systems in many countries. Indeed this study was carried out to analyse chemical constituents of some medicinal plants used to treat reproductive organs, diseases in Bamboutos division, West of Cameroon. 45 plants samples were collected from their natural habitats in some villages of the above cited division. Study was conducted according to standard procedures in the laboratory of Organic Chemistry and Natural Substances of the University of Dschang. Polyphenols were revealed in all screening medicinal plant extracts, while, flavonoids were found in most of the studied plant extracts except for those obtained from Dioscorea dumetorum. Triterpens were present in 40 plants, saponins and sterols were respectively found in 33 and 32 plants ; whereas, alkaloids were only present in 15 plants.This work showed that the plants used to alleviate human reproductive ailments in some locality of the West Region in Cameroon are rich in bioactive compounds. These compounds are known to manage human reproductive diseases. Therefore, this finding supported the uses of these plant species for the treatment of human reproductive diseases. Furthermore, appropriate conservation of such valuable medicinal plant species is required.
PubDate: 2022-05-10
DOI: 10.9734/jocamr/2022/v18i130343
|
Open Access journal
