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- Chemical composition, antioxidant and antibacterial activities of Brocchia
Cinerea from South-East of Morocco
Authors: Aicha Hamdouch, Hasna Ait Bouzid, SAID GHARBY, Ali Asdadi, Fouad Achemchem, Bouchra Chebli, Lalla Mina Idrissi Hassani
Pages: 1 - 20
Abstract: The essential oil of wild-growing medicinal specie Brocchia cinerea from oasis of Tata (Southeast of Morocco) was studied for chemical composition, antioxydant and antibacterial activities. The essential oil is extracted using a clevenger distillation apparatus. The antioxidant activity was determined by measuring the free radical scavenging power of DPPH and the antibacterial activity by broth dilution method Gas chromatography-mass spectrometry (GC/MS) results revealed that the major compounds of Brocchia cinerea are Thujone, Camphor and Eucalyptol. The antibacterial test of Brochia cinerea essential oil revealed a strong antibacterial activity against Listeria innocua, Pseudomonas aeruginosa and Staphylococcus aureus (MIC<1 µL) and also a high scavenging effect of DPPH (IC50=0.080± 0.014).
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.28092
Issue No: Vol. 8, No. 1 (2022)
- Phytochemical profile, antioxidant and antibacterial activities of Psidium
guajava, Cassia occidentalis, Euphorbia hirta, and Todalia asiatica
growing in Comoros islands
Authors: Mohammed LACHKAR, Abdelmoughite Ouakil, Hamidou Hamada Soule, Ouafaa El Mahdi, Hanane El Hajaji, Fadoua Bennouna, Soumya El Abed, Saad Ibnsouda Koraichi, Brahim El Bali
Pages: 21 - 40
Abstract: This work aimed to evaluate the phytochemical content and to determine the antioxidant and antimicrobial activities of Psidium guajava, Cassia occidentalis, Euphorbia hirta, and Todalia asiatica extracts. The antioxidant capacities of organic solvent extracts were examined by three different methods, namely, free radical scavenging activity DPPH method, reducing power scavenging activity, and total antioxidant capacity. All tests showed significant dose-dependent antioxidant activity. For DPPH radical scavenging activity and reducing power assay, it is the E. hirta extract, which manifested the highest activity. However, for the total antioxidant capacity, the stronger activity was showed by P. guajava extract and ethyl acetate extract of T. asiatica. In vitro antimicrobial studies of methanol extracts were carried out against four medically important microbial strains, including Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas using disc diffusion assay. The extracts showed a low effect on bacterial growth inhibition.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.28820
Issue No: Vol. 8, No. 1 (2022)
- Antifungal activity of essential oil from Santolina pectinata lag.,
against postharvest phytopathogenic fungi in apples
Authors: Manssouri Mounir, Ou-Ani Omar, Oucheikh Lahcen, Laghchimi Amal, Ansari Abdeslam, Lhou Majidi, Znini Mohamed
Pages: 41 - 54
Abstract: The purpose of this study was to investigate the chemical composition and antifungal activities of essential oil (EO) of Santolina pectinata aerial parts. The analysis of EO using gas chromatography (GC-FID) and gas chromatography-mass spectroscopy (GC-MS) allowed the identification of 34 components, which (Z)-heptadeca-10,16-dien-7-one was the major constituent with 28 % of the total oil. The antifungal activities of this oil in liquid and vapor-phase against three phytopathogenic fungi causing the deterioration for apples, including Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer were evaluated in vitro by using the poisoned food (PF) and the volatile activity (VA) methods. The obtained results showed a significant inhibition of the studied EO inhibited significatively against the mycelial growth of all fungal strains tested (p<0.05). B. cinerea and P. expansum showed a strong sensitivity to the studied oil at all concentrations and VA assay was consistently found to be more effective than PF technique. The minimum inhibitory concentrations (MICs) of this oil were 0. 25 and 1 µL/mL against these strains when using VA and PF assays, respectively. The overall results suggest that S. pectinata EO could be used as an antifungal preservative to control post-harvest diseases of apples. The antifungal activities of S. pectinata EO from Morocco are reported for the first time.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.26656
Issue No: Vol. 8, No. 1 (2022)
- Gas Chromatography-Mass Spectrometry Analysis and Comparison of Volatile
Components Obtained by Hydrodistillation and Headspace Solid Phase
Microextraction (HS-SPME) from Teucrium luteum subsp. flavovirens
Authors: Mohamed ZNINI, Omar Ou-Ani, Driss Chebabe, jean Costa, Lhou Majidi
Pages: 55 - 67
Abstract: The main purpose of this work is to investigate the comparative chemical analysis of essential oil (EO) isolated by hydrodistillation (HD) and volatile fraction (VF) detected by headspace solid-phase micro-extraction (HS–SPME) obtained from Teucrium luteum subsp. flavovirens using Gas Chromatography–Retention Indices (GC-RI) and GC–Mass Spectrometry (GC-MS). 63 volatile compounds identified in hydrodistilled essential oil (HD), representing 89.9% of the total oil, while HS-SPME revealed 50 components constituting 99.3% of the volatile material. The chemical composition of EO comprised mainly of of oxygenated sesquiterpenes (48.6%) while, hydrocarbon monoterpenes were detected in higher concentrations in VF (54.1%). The comparative analysis of two chemoprofiles obtained by two methods shows quantitative and semi-qualitative differences. The current study is the first report involving rapid analysis of volatile components of T. luteum subsp. flavovirens by HS-SPME.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.28536
Issue No: Vol. 8, No. 1 (2022)
- Plants Mixture Approach: Effect of extraction methods on Polyphenolic
Content of Some Medicinal Plants Used against Oral Disorder
Authors: Hazim HAROUAK, Jamal IBIJBIJEN, laila NASSIRI
Pages: 68 - 86
Abstract: On the basis of an ethnobotanical survey with herbalists, who cites plants in a mixed way against dental disorders, comes the idea of this article, for tests of total phenolic compounds the colorimetric method Folin-Ciocalteu, for flavonoids content the standard catechin and for condensed tannins the acidified vanillin was used. Three methods were also used for crude extractions; namely: infusion, decoction and Soxhlet techniques, the results were statistically analyzed and different correlations between samples and the inter samples were made; the results showed the antagonistic effect in these mixtures where their content decreases compared to plants alone, the phenolic profitability is observed in the mixtures containing less plants extracted by Soxhlet, contrary to the flavonoic compounds which are more in the mixtures containing more plants where their profitability is proportionally related to the extraction by infusion, but at condensed tannins level a specific plant in a specific mixture which can be richer in these compounds, the different correlations have approached these results which can be the object of a more interesting study at the level of the chemical reactions which is carried out from one family to another and from mixture to another.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.27042
Issue No: Vol. 8, No. 1 (2022)
- Citrus ×Clementina (leaf). Total Phenolic, Flavonoids contents,
Antioxidant activity and chemical composition of their essential oil
Authors: Soukaina SAIDI, Nadia Handaq, Firdaous Remok, Aziz Drioiche, Latifa Bouissane, Touriya Zair
Pages: 87 - 107
Abstract: The petitgrain 'Citrus*clementina' is an aromatic plant classed as a green waste. Even though it has benefits, few researchers studied it. The aim of this study is to extract the essential oils from the leaves of C. clementina by using two methods, the conventional hydrodistillation and the steam distillation, and to evaluate the content of phenolic compounds and the antioxidant activities of their extracts.The constituent of volatile compounds obtained by conventional hydrodistillation (Heh) and steam distillation (HEv) methods were analyzed by (GC-MS) and their results were compared.The major compounds of their essential oils were sabinene, linalool, limonene, carina <δ-3-> and ocimene <(E)-β->.The chemical composition of essential oil from each method were compared and found to be similar.Decoction and Soxhlet are the used methods for extract of phenolic compounds. The aqueous and hydro-ethanolic extracts from the Soxhlet method registered the highest phenolic contents.The antioxidant activity of C.clementina extracts were performed by ferric reducing antioxidant power (FRAP) and total antioxidant capacity (CAT). The results indicate that the decocted and hydroethanolic extracts show a greater antioxidant capacity compared to the aqueous extract.Thus, this result is a relevant source of bioactive compounds with antioxidant properties of interest for consumers and cooperatives as well as participating in socioeconomic development.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.27101
Issue No: Vol. 8, No. 1 (2022)
- Antifungal activity of the essential oils of Rosmarinus officinalis,
Salvia officinalis, Lavandula dentata and Cymbopogon citratus against the
mycelial growth of Fusarium oxysporum f.sp.Albedinis
Authors: BARKAOUI Hassan, CHAFIK Zouheir, BENABBAS Redouane, CHETOUANI Mohammed, EL MIMOUNI Mustapha, HARIRI Elbachir
Pages: 108 - 133
Abstract: The present study concerns the antifungal activity of essential oils (EO) of four aromatic plants: Salvia officinalis, Rosmarinus officinalis, Cymbopogon citratus and Lavandula dentate. It was carried out on the mycelial growth of Fusarium oxysporum f.sp.Albedinis. The essential oils were extracted from the aerial parts of the plants by the hydrodistillation method. The test was carried out using the method of direct contact with the fungus at different concentrations of essential oils (0.25%, 1% and 2.5%).The results show that the essential oils of lavender and lemon grass exert a strong antifungal activity on the fungus at a concentration of 2.5 g/l for the essential oil of lavender and at a concentration of 1g/l for the essential oil of lemon grass. On the other hand, the two essential oils of sage and rosemary proved to have a lesser impact on the tested strain. The minimum inhibitory concentrations (MIC) were observed in the four essential oils including, MIC(Rosmary)= 0.2 g/l, MIC(Sage)= 2.5 g/l, MIC(Lavender)= 0.6 g/ l and MIC(Lemon grass)= 0.5g/l. The minimum lethal concentrations (MLC) were observed only in lavender essential oil, with MLC(Lavander)= 1.75g/l and in lemongrass essential oil, with MLC(Lemon grass)= 0.95 g/l .
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.30676
Issue No: Vol. 8, No. 1 (2022)
- Effects of cutting origin and exogenous auxin treatment on the rooting of
Authors: AITHIDA Amal, TAHIRI Abdelghani, AZLAY Latifa, IDRISSI HASSANI L. Amina, MOKHTAR Mimoun
Pages: 134 - 154
Abstract: The Rosa damascena (Mill), commonly known as Damask rose, is one of the most important Rosa species with great economic interest on a national and international scale. In Morocco, especially in M’goun and Dades valleys, the Damask rose is mainly propagated by replanting rooted shoots. However, this method is presenting several constraints. Considering this fact, this study was undertaken to investigate the influence of two major factors: the origin of cutting and the effect of the auxin treatment on Rosa damascena cutting. For this purpose, the cuttings from seven localities were cultivated in a greenhouse under controlled conditions after soaking treatment in different concentrations of Butyric Indole Acid (0; 2000 and 4000 ppm). The obtained results revealed a significant (P<0,01) difference between the seven localities for the focused parameters. In fact, the individuals belonging to the same morphotype act in the same way with regard to cutting. The rooting rate varies between 10% and 70% depending on the origin of cuttings and the applied concentration of IBA. The results of this study showed that propagation of Rosa damascena by cuttings can promote this culture. However, the optimization of this method is linked to the choice of the plant, the origin of cuttings in particular to the chosen ecotypes.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.30674
Issue No: Vol. 8, No. 1 (2022)
- The use of Ulva lactuca seaweed for post harvesting control of Penicillium
digitatum in citrus fruits
Authors: Josephine AL ALAM, Douaa Salim, Ziad Fajloun, Maurice Millet, Asma Chbani
Pages: 155 - 170
Abstract: Seaweed liquid extracts (SLE) were assessed as natural antifungal products for the control of the post-harvest citrus green mold Penicillium digitatum. The SLE of a green alga, Ulva lactuca, at different concentration (25, 50, 100 and 200 g L-1) were evaluated for their inhibitory potential of the in vivo development of P. digitatum on fresh oranges and of the in vitro conidia germination and tube elongation of the mold on potato dextrose broth (PDB). The study of their effects on fungus viability on malt extract agar (MEA) was also evaluated. The results revealed that the assessed SLE at 50 and 100 g L-1 allowed a potential inhibition of the fungus viability and development. The U. lactuca liquid extract at 50 g L-1 allowed a complete in vivo protection of the oranges from the mold for the total duration of the study and a 90 % inhibition of spore germination. Moreover, the chemical characterization of the extract showed valuable content in polysaccharides and mainly in ulvan, known for its antifungal effects. These findings could be highly promising to develop an eco-friendly, safe and effective product for citrus fruit post-harvesting protection.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.27265
Issue No: Vol. 8, No. 1 (2022)
- Influence of maturity stage on chemical composition and antioxidant
activity of Pistacia lentiscus seed oils
Authors: Brahim AIT MOHAND, Abderraouf EL ANTARI, Fatiha BENKHALTI
Pages: 171 - 186
Abstract: Quality plays a key role in the marketing of vegetable oils. This quality is influenced by a number of factors, including variety, crop management, date of harvest, place of cultivation and extraction procedure. The objective of our study was to determine the ideal seed harvest period to reach their full potential of quality by analyzing the variation of chemical quality and antioxidant activity of Pistacia lentiscus seed oil in three different stages of maturation. The results obtained in this study indicate that as maturity progressed, there was a decrease in acidity (7.13-2.61%), chlorophyll content (24.43 - 4.69 ppm), and the total phenol content (808.22 - 191.24 mg GAE / kg). while there was an increase the oil content (11.07- 33.4%) and the composition of monounsaturated fatty acids (44.36- 54.43%), mainly oleic acid (43.11- 52.01%). Oil extracted from first stage (green seeds) showed the highest percentage of DPPH inhibition with around 57.56% followed by red seeds with a DPPH inhibition percentage of 49.99%. Earlier harvest would produce lentisk oils of good quality, stable and a composition rich in natural substances. The optimal stage of harvest is proposed by considering the compromise between the quantity and the quality of the studied parameters.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.27039
Issue No: Vol. 8, No. 1 (2022)
- Effect of phenological stages on yield, chemical composition and
biological properties of essential oil from Thymus maroccanus Ball.
Authors: A. Aljaiyash, H. Labiad, C Alaoui, M. Ghanmie, B. Satranie
Pages: 187 - 208
Abstract: The effects of phenological stages on the composition, antioxidant, insecticidal and allelopathic properties of essential oils (EOs) obtained from the aerial parts of Thymus maroccanus Ball. were evaluated. The GC-MS analysis identified that analyzed EOs were dominated by carvacrol (63.70 - 68.19 %) followed by p-cymene (6.09 - 9.67 %) and γ-terpinene (3.67 - 8.49 %). When carvacrol and p-cymene gradually increased from the lowest values (63.70% and 6.09%) at the pre-flowering stage to reach the highest ones (68.19% and 9.67%) at post-flowering stage, γ-terpinene gradually decreased from the highest proportion (8.49%) to the lowest (3.67%). EO from post-flowering aerial parts showed higher scavenging ability on DPPH radicals (% inhibition: 25.19 - 95.75 %; IC50 = 0.26 mg/mL), in contrast, the essential oil from pre-flowering stage exhibited the highest Fe3+ reducing power ability (Absorbance: 0.329 - 0.812; IC50 = 0.14 mg/mL). Insecticidal properties showed no substantial difference between T. maroccanus EO extracted at different growth stages. The LD50 and LD90 values were ranged from 0.15 to 0.17 µL/cm2, and from 0.37 to 0.47 µL/cm2, respectively in contact assays, as well as from 318.93 to 362.84 µL/L air, and from 725.08 to 739.12 µL/L air, respectively in fumigant assays. The results indicated that allelopathic activity of Eos is not greatly affected by growth developmental stages.
PubDate: 2022-02-10
DOI: 10.48347/IMIST.PRSM/ajmap-v8i1.30673
Issue No: Vol. 8, No. 1 (2022)
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