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  Subjects -> ALTERNATIVE MEDICINE (Total: 106 journals)
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African Journal of Traditional, Complementary and Alternative Medicines
Number of Followers: 3  

  This is an Open Access Journal Open Access journal
ISSN (Print) 0189-6016
Published by African Journals Online Homepage  [261 journals]
  • Antimicrobial and antiradical properties of Hammada scoparia
           (Pomel) Iljin

    • Authors: Aziz Drioichea, Nadia Benhlima, Samira Kharchoufa, Fadoua El-Makhoukhi, Smahane Mehanned, Imad Adadi, Hicham Aaziz, Ferdinand Kouoh Elombo, Bernard Gressier, Bruno Eto, Touriya Zaira
      Pages: 1 - 14
      Abstract: Background: Hammada scoparia (Pomel) Iljin (HS), is commonly used by traditional healers in Morocco against microbial and fungal infections. We studied antimicrobial, antifungal and antiradical effects of organic extracts in vitro in order to confirm traditional utilization after phytochemical screening.
      Materials and methods: Aerial parts of HS have been extracted by hydro-distillation using Clevenger-type apparatus, and the chemical composition was realized by Gas Chromatography coupled with Mass Spectroscopy (GC/MS). The antioxidant activity has been evaluated using DPPH test, while the antimicrobial tests of HS extract were conducted on twenty-eight bacterial strains and antifungal on twelve fungal strains.
      Results: Chemical characterization of HS essential oils (EO) confirmed the presence of carvacrol (82,28%), p-cymene (2,52%), γ- terpinene (2,18%) and Z-caryophyllene (2,04%). Antimicrobial tests of HS extract showed a moderate antibacterial activity without antifungal effect. In addition, HS exhibited a very powerful antiradical activity with IC50 = 1,2 mg/ml compared to that of ascorbic acid (IC50 = 0,5 mg/mL) and butylated  hydroxyanisole (0,8 mg/mL).
      Conclusion: To our knowledge, this is the first demonstration that HS directly inhibits the growth of microorganisms in vitro, and further validates its traditional use as an antiseptic by traditional Moroccan healers. Keywords: polyphenolics, Hammada scoparia, antimicrobial, antifungal, antioxidant.
      PubDate: 2020-04-30
      DOI: 10.4314/ajtcam.v16i2.
      Issue No: Vol. 16, No. 2 (2020)
  • Evaluation of antibacterial activity of medicinal plant extracts against
           clinical isolates of pathogens from children with acute gastroenteritis at
           Katutura State Hospital¸ Windhoek, Namibia

    • Authors: Albertina M.N. Iikasha, Isaac K. Quaye, Davis R. Mumbengegwi
      Pages: 15 - 23
      Abstract: Background: Diarrhea is a major health concern in Namibia with an estimate of 17000 cases in 2015 in Omusati and Kunene regions. The present study aimed at isolating and identifying gastrointestinal bacteria from stool samples of children admitted at Katutura hospital, Windhoek, and to determine the efficacy of selected medicinal plants used in treating gastroenteritis in Namibia. Materials and Methods: Stool samples were collected for 6 months from children under age five admitted with acute diarrhea. Seeplex12 automated DNA extractor and PCR were used for isolation and identification of bacterial DNA from the samples. Serially diluted stool samples were cultured on selective media, sub-cultured in Nutrient broth and preserved in 80% glycerol. Parts of Boscia albitrunca, Ziziphus mucronata, Combretum apiculatum, Solanum linnaeanum and Terminalia sericea were collected, dried, ground and extracted using distilled water and ethanol. Disc diffusion method was used to determine the antibacterial activity of the plant extracts against isolated bacteria. Results: A total of 12 out of the 18 stool samples collected had bacterial pathogens successfully identified by PCR. 33.3% were positive for Salmonella, 11.1% for E. coli 157:H7 VTEC and 22.2% for E.coli H7. C. apiculatum organic extracts exhibited potent antibacterial activity of (16±  0.57mm) at 1000 μg/ml against Shigella and Salmonella with a moderate minimum inhibitory concentration (MIC) of 250 μg/ml against  Salmonella. Salmonella and Shigella showed resistance to 10 μg/ml of ampicillin. Conclusion: Plant extracts showed in vitro antibacterial activity. However, toxicology and in vivo efficacy of these plant extracts should be  determined before recommending their mainstream uses. Key words: Acute Diarrhea, Medicinal Plants, Antibacterial, Resistance, Minimum Inhibitory Concentration.
      PubDate: 2020-04-30
      DOI: 10.4314/ajtcam.v16i2.
      Issue No: Vol. 16, No. 2 (2020)
  • Proapoptotic, anti-cell proliferative, anti-inflammatory and
           antiangiogenic potential of carnosic acid during 7,12
           dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis

    • Authors: D. Rajasekaran , S. Manoharan , S. Silvan , K Vasudevan , N. Baskaran , D. Palanimuthu
      Pages: 24 - 25
      Abstract: ERRATUM
      Rajasekaran et al., Afr., J. Tradit Complement Altern Med. 2013; 10(1): 102–112
      Published online 2012 Oct 1.
      doi: 10.4314/ajtcam.v10i1.14
      PubDate: 2020-04-30
      DOI: 10.4314/ajtcam.v10i1.14
      Issue No: Vol. 16, No. 2 (2020)
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