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  Subjects -> ALTERNATIVE MEDICINE (Total: 106 journals)
Showing 1 - 15 of 15 Journals sorted alphabetically
Acupuncture & Electro-Therapeutics Research     Full-text available via subscription   (Followers: 3)
Acupuncture in Medicine     Hybrid Journal   (Followers: 16)
Advanced Herbal Medicine     Open Access   (Followers: 8)
Advances in Traditional Medicine     Hybrid Journal   (Followers: 3)
African Journal of Traditional, Complementary and Alternative Medicines     Open Access   (Followers: 3)
Akupunktur & Aurikulomedizin     Full-text available via subscription   (Followers: 1)
Allgemeine Homöopathische Zeitung     Hybrid Journal   (Followers: 1)
Alternative & Integrative Medicine     Open Access   (Followers: 10)
Alternative Medicine     Full-text available via subscription   (Followers: 2)
Alternative Medicine Studies     Open Access   (Followers: 6)
Anales de Hidrología Médica     Open Access   (Followers: 1)
Ancient Science of Life     Open Access   (Followers: 3)
Arteterapia. Papeles de arteterapia y educación artística para la inclusión social     Open Access   (Followers: 3)
Asian Journal of Plant Pathology     Open Access   (Followers: 5)
Australian Journal of Acupuncture and Chinese Medicine     Full-text available via subscription   (Followers: 2)
Australian Journal of Herbal Medicine     Full-text available via subscription   (Followers: 4)
Australian Journal of Music Therapy     Full-text available via subscription   (Followers: 9)
Avicenna Journal of Phytomedicine     Open Access   (Followers: 1)
AYU : An international quarterly journal of research in Ayurveda     Open Access   (Followers: 4)
BMC Complementary Medicine and Therapies     Open Access   (Followers: 17)
Chinese Herbal Medicines     Full-text available via subscription   (Followers: 1)
Chinese Medicine     Open Access   (Followers: 3)
Chinese Medicine and Culture     Open Access   (Followers: 1)
Cognitive Neuroscience     Hybrid Journal   (Followers: 31)
Complementary Therapies in Clinical Practice     Hybrid Journal   (Followers: 18)
Complementary Therapies in Medicine     Hybrid Journal   (Followers: 16)
Deutsche Heilpraktiker-Zeitschrift     Hybrid Journal   (Followers: 2)
Erfahrungsheilkunde     Hybrid Journal   (Followers: 1)
European Journal of Medicinal Plants     Open Access   (Followers: 2)
Evidence-based Complementary and Alternative Medicine     Open Access   (Followers: 22)
Experimental and Therapeutic Medicine     Full-text available via subscription  
Fitoterapia     Hybrid Journal   (Followers: 4)
Focus on Alternative and Complementary Therapies     Hybrid Journal   (Followers: 11)
Global Journal of Integrated Chinese Medicine and Western Medicine     Open Access  
Global Journal of Traditional Medicine     Open Access  
Herba Polonica     Open Access  
Herbal Medicines Journal     Open Access   (Followers: 1)
Indian Journal of Ayurveda and lntegrative Medicine Klue     Open Access   (Followers: 3)
Indian Journal of Research in Homoeopathy     Open Access  
Indian Journal of Traditional Knowledge (IJTK)     Open Access   (Followers: 1)
Innovare Journal of Ayurvedic Sciences     Open Access   (Followers: 2)
Intas Polivet     Full-text available via subscription  
Integrative Medicine Research     Open Access   (Followers: 3)
International Journal of High Dilution Research     Open Access  
International Journal of Qualitative Studies on Health and Well-Being     Open Access   (Followers: 21)
International Journal of Yoga     Open Access   (Followers: 15)
International Journal of Yoga : Philosophy, Psychology and Parapsychology     Open Access   (Followers: 6)
Ipnosi     Full-text available via subscription  
Journal of Acupuncture and Tuina Science     Hybrid Journal   (Followers: 4)
Journal of Alternative and Complementary Medicine     Hybrid Journal   (Followers: 14)
Journal of Applied Arts and Health     Hybrid Journal   (Followers: 1)
Journal of Applied Research on Medicinal and Aromatic Plants     Hybrid Journal   (Followers: 1)
Journal of Asian Natural Products Research     Hybrid Journal   (Followers: 5)
Journal of Ayurveda     Open Access   (Followers: 3)
Journal of Ayurveda and Integrative Medicine     Open Access   (Followers: 6)
Journal of Ayurveda Case Reports     Open Access   (Followers: 3)
Journal of AYUSH :- Ayurveda, Yoga, Unani, Siddha and Homeopathy     Full-text available via subscription   (Followers: 7)
Journal of Bodywork and Movement Therapies     Hybrid Journal   (Followers: 16)
Journal of Complementary and Alternative Medical Research     Open Access  
Journal of Dance Medicine & Science     Full-text available via subscription   (Followers: 10)
Journal of Drug Research in Ayurvedic Sciences     Open Access   (Followers: 3)
Journal of Evidence-Based Integrative Medicine     Open Access   (Followers: 18)
Journal of Fasting and Health     Open Access   (Followers: 1)
Journal of Ginseng Research     Open Access  
Journal of Health Science and Alternative Medicine     Open Access   (Followers: 1)
Journal of Health Sciences Scholarship     Open Access  
Journal of Herbal Drugs (An International Journal on Medicinal Herbs)     Open Access  
Journal of Herbal Medicine     Hybrid Journal   (Followers: 2)
Journal of Herbal Science     Full-text available via subscription   (Followers: 3)
Journal of Integrative Medicine     Full-text available via subscription   (Followers: 2)
Journal of Integrative Medicine & Therapy     Open Access  
Journal of Manual & Manipulative Therapy     Hybrid Journal   (Followers: 17)
Journal of Medicinal Plants for Economic Development     Open Access   (Followers: 1)
Journal of Medicinally Active Plants     Open Access   (Followers: 2)
Journal of Natural Remedies     Open Access   (Followers: 3)
Journal of Nutraceuticals and Herbal Medicine     Open Access  
Journal of Palliative Medicine     Hybrid Journal   (Followers: 51)
Journal of Research in Ayurvedic Sciences     Open Access   (Followers: 3)
Journal of the Australian Traditional-Medicine Society     Full-text available via subscription  
Journal of Traditional and Complementary Medicine     Open Access   (Followers: 3)
Journal of Traditional Chinese Medical Sciences     Open Access  
Médecine Palliative     Full-text available via subscription   (Followers: 1)
Medical Acupuncture     Hybrid Journal   (Followers: 5)
Medicines     Open Access   (Followers: 1)
Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi     Open Access  
Muller Journal of Medical Sciences and Research     Open Access   (Followers: 1)
Natural solutions     Full-text available via subscription  
Natural Volatiles & Essential Oils     Open Access  
Nigerian Journal of Natural Products and Medicine     Full-text available via subscription  
OA Alternative Medicine     Open Access  
Oriental Pharmacy and Experimental Medicine     Partially Free   (Followers: 2)
Research Journal of Medicinal Plant     Open Access   (Followers: 2)
Research Journal of Pharmacognosy     Open Access  
Revista Internacional de Acupuntura     Full-text available via subscription  
South African Journal of Plant and Soil     Hybrid Journal   (Followers: 1)
Synfacts     Hybrid Journal   (Followers: 5)
Traditional & Kampo Medicine     Full-text available via subscription  
Traditional Medicine Journal     Open Access  
World Journal of Acupuncture - Moxibustion     Full-text available via subscription  
World Journal of Traditional Chinese Medicine     Open Access  
Yoga Mimamsa     Open Access   (Followers: 2)
Zeitschrift für Orthomolekulare Medizin     Hybrid Journal   (Followers: 1)

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Acupuncture in Medicine
Journal Prestige (SJR): 0.702
Citation Impact (citeScore): 1
Number of Followers: 16  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0964-5284 - ISSN (Online) 1759-9873
Published by BMJ Publishing Group Homepage  [62 journals]
  • Manual acupuncture at ST36 attenuates rheumatoid arthritis by inhibiting
           M1 macrophage polarization and enhancing Treg cell populations in
           adjuvant-induced arthritic rats

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      Authors: Nannan Yu, Fuming Yang, Xue Zhao, Yongming Guo, Yuan Xu, Guangchang Pang, Yinan Gong, Shenjun Wang, Yangyang Liu, Yuxin Fang, Kun Yu, Lin Yao, Hui Wang, Kuo Zhang, Baohu Liu, Zhenguo Wang, Yi Guo, Zhifang Xu
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objectives:Acupuncture has been found to be effective at relieving many inflammatory pain conditions, including rheumatoid arthritis (RA). We aimed to assess the anti-inflammatory potential of manual acupuncture (MA) treatment of RA using adjuvant-induced arthritic (AIA) rats and to explore the underlying mechanisms.Methods:The anti-inflammatory and analgesic actions of MA at ST36 (Zusanli) in AIA rats were assessed using paw withdrawal latency and swelling, histological examination and cytokine detection by enzyme-linked immunoassay (ELISA). The cell–cell communication (CCC) network was analyzed with a multiplex immunoassay of 24 immune factors expressed in the inflamed joints, and the macrophage and Treg populations and associated cytokines regulated by MA were investigated using reverse-transcription quantitative polymerase chain reaction (RT-qPCR), ELISA and flow cytometry.Results:MA markedly decreased heat hyperalgesia and paw swelling in AIA rats. MA-treated rats also exhibited decreased levels of pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β) coupled with increased anti-inflammatory cytokines (IL-10, transforming growth factor (TGF)-β1) in the ankle joints at protein and mRNA levels. CCC network analysis confirmed that macrophages are of critical importance and are potential therapeutic targets in RA. Repeated treatment with MA triggered a macrophage phenotypic switch in the paws, with fewer M1 macrophages. Prominent increases in the Treg cell population and TGF-β1 in the popliteal lymph nodes demonstrated the immunomodulatory effects of MA. Furthermore, a selective TGF-β1-receptor inhibitor, SB431542, attenuated the anti-inflammatory effects of MA and MA-induced suppression of the levels of M1-released cytokines.Conclusion:These findings provide novel evidence that the anti-inflammatory and analgesic effects of MA on RA act through phenotypic modulation involving the inhibition of M1 macrophage polarization and an increase in the Treg cell population, highlighting the potential therapeutic advantages of acupuncture in controlling pain and ameliorating inflammatory conditions.
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-19T05:50:42Z
      DOI: 10.1177/09645284221085278
       
  • Electroacupuncture for brachial plexus injury caused by fracture of the
           right greater tuberosity of the humerus and dislocation of the right
           shoulder joint: a case report

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      Authors: JianZhu Wang, LiJun Wei, GuangLin Li, YunFan Bao, YanDing Tang, Li Zhang, QiPing Zu, HanYu Zhou, Jing Wang
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T12:53:31Z
      DOI: 10.1177/09645284221085578
       
  • Treatment of acute exacerbations of chronic obstructive pulmonary disease
           with acupuncture during hospitalization: a three-arm double-blinded
           randomized sham-controlled trial

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      Authors: Ilana Levy, Yotam Elimeleh, Sagi Gavrieli, Samuel Attias, Ariel Schiff, Arie Oliven, Elad Schiff
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are a healthcare burden. Acupuncture improves dyspnea in patients with chronic obstructive pulmonary disease (COPD) but, to the best of our knowledge, has not been tested in AECOPD. Here, we evaluated the efficacy and safety of true acupuncture added to standard of care (SOC), as compared with both sham procedure plus SOC and SOC only, for the treatment of AECOPD among inpatients.Methods:This double-blinded randomized sham-controlled trial was set in a tertiary hospital in Israel. Patients with a clinical diagnosis of AECOPD were assigned to true acupuncture with SOC, sham procedure with SOC or SOC only. The primary outcome was dyspnea improvement as measured daily by the validated modified Borg (mBorg) scale. Secondary outcomes included improvement of other patient-reported outcomes and physiologic features, as well as duration of hospitalization and treatment failure. Acupuncture-related side effects were evaluated by the validated Acup-AE questionnaire.Results:Seventy-two patients were randomized: 26 to acupuncture treatment, 24 to sham and 22 to SOC only arms. Baseline characteristics were similar in the three groups. A statistically significant difference in dyspnea intensity was found from the first day of evaluation after treatment (p = 0.014) until day 3 after treatment. Similar results were found for sputum production, but no statistical significance was found when comparing physiologic features between the three arms. Acupuncture was not associated with adverse events.Conclusion:Acupuncture seems to be efficacious in the treatment of AECOPD among inpatients hospitalized in internal medicine departments.Trial registration number:NCT03398213 (ClinicalTrials.gov)
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:57:51Z
      DOI: 10.1177/09645284221086293
       
  • Efficacy and safety of acupuncture as a complementary therapy for sepsis:
           a systematic review and meta-analysis

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      Authors: Jin Xian, Ling Wang, Changyun Zhang, Jian Wang, Yushuo Zhu, Huijuan Yu, Xin Zhang, Qiwen Tan
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Sepsis is a life-threatening organ dysfunction caused by dysregulation of the host response to infection. Acupuncture is used for treatment of inflammatory diseases; however, its effectiveness and safety as a complementary therapy for sepsis has not been fully explored.Methods:Data were retrieved from eight databases. Randomized controlled trials (RCTs) that compared acupuncture plus conventional therapies versus conventional therapies alone were included. Pre-specified primary outcomes were mortality at 28 days and Acute Physiologic and Chronic Health Evaluation (APACHE) II scores.Results:A total of 17 studies with 1099 participants were included in this study. In terms of the primary outcomes, acupuncture plus routine therapy reduced mortality at 28 days (risk ratio (RR)): 0.69, 95% confidence interval (CI): 0.52 to 0.91, p < 0.001) and APACHE II scores (mean difference (MD): −2.84, 95% CI: −4.09 to −1.58, p < 0.001) at day 7 after treatment compared with routine therapy alone. In terms of secondary outcomes, acupuncture plus routine therapy reduced white blood cell counts and levels of procalcitonin (PCT), tumor necrosis factor (TNF)-α, interleukin (IL)-6 and lactic acid and intra-abdominal pressure (IAP), and improved CD3+, CD4+ and monocytes of human leukocyte antigen (HLA)-DR at day 7 after treatment compared with routine therapy alone. However, acupuncture plus routine therapy had no significant effects on levels of IL-10, C-reactive protein (CRP), CD8+ and CD4+/CD8+ ratios compared with routine therapy alone. Quality of evidence was low to very low for all parameters (GRADE).Conclusion:The available evidence showed that combination of acupuncture and routine therapy may have benefit for sepsis compared with use of routine therapy only. Due to the low degree of certainty regarding its effects, further research is required.Trial registration number:ICRD42019141491 (PROSPERO).
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:53:51Z
      DOI: 10.1177/09645284221086288
       
  • Sensory and autonomic innervation of the local tissues at traditional
           acupuncture point locations GB14, ST2 and ST6

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      Authors: Jia Wang, Jing-jing Cui, Dong-sheng Xu, Yu-xin Su, Jie-ying Liao, Shuang Wu, Ling Zou, Ya-ting Guo, Yi Shen, Wan-zhu Bai
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:To visualize and compare the sensory and autonomic innervation of the local tissues at the sites of different traditional acupuncture points in the rat forehead and face by histochemical examination.Methods:GB14 (Yangbai), ST2 (Sibai) and ST6 (Jiache) were selected as the representative traditional acupuncture points in this study, and the local tissues at these sites were dissected in rats after perfusion followed by double or triple fluorescent histochemical staining. Here, calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH) and vesicular acetylcholine transporter (VAChT) were used to label the sensory, sympathetic and parasympathetic nerve fibers, respectively.Results:The CGRP+ sensory, TH+ sympathetic and VAChT+ parasympathetic nerve fibers were simultaneously demonstrated in the local tissues at GB14, ST2 and ST6. Although the three kinds of nerve fibers ran in parallel or intermingled with each other, by the analysis from the view of three-dimensional reconstruction, it was clear that each of them distributed in an independent pattern to their corresponding target tissues including the blood vessels, hair follicles, arrector pili and subcutaneous muscles, as well as sebaceous glands.Conclusion:Our study demonstrated the sensory and autonomic innervation of the local tissues at GB14, ST2 and ST6, providing neurochemical evidence indicating that the CGRP+ sensory, TH+ sympathetic and VAChT+ parasympathetic nerve fibers form a neural network at these point locations that may respond to acupuncture stimulation.
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:51:43Z
      DOI: 10.1177/09645284221085579
       
  • Influence of acupuncture needle physical–chemical properties on
           needling quality

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      Authors: Rafael Yamaguti Lenoch, Ari Ojeda Ocampo More, Patricia Ortega Cubillos, Li Shih Min, Ari Digiácomo Ocampo More, Carlos Rodrigo de Mello Roesler
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objectives:This study analyzed the physical–chemical properties of three different brands of acupuncture needle, classified by acupuncturists as high (A), medium (B) and low (C) quality.Methods:Experienced acupuncturists, rated, in terms of perceived needling quality, three acupuncture needle brands as high (A), medium (B) and low (C) quality. Next, scanning electron microscopy (SEM) images of the tip and surface finish of the needles of each brand were analyzed. A mechanical test was developed and performed to evaluate the compressive force required to insert the needles through a smooth surface (silicon). In addition, X-ray fluorescence spectroscopy (XRF) and dispersive energy spectroscopy (DES) were conducted to analyze the material composition of the needles and presence of oxidation.Results:SEM images revealed that needle brands A and B presented a sharper tip and a more regular surface finish in comparison to brand C. In the insertion test, needle brands A and B had similar performance characteristics, with A requiring less force to penetrate the silicon device when compared to B, while C failed to penetrate the silicon and complete the test. The XRF analysis did not reveal any differences in material composition between the three brands. However, brand C exhibited particles embedded on the needle surface and DES confirmed oxidation.Conclusion:This study demonstrates that perceived needling quality by acupuncturists can be correlated with physical–chemical properties of the needles, especially those related to finishing quality of the tip and the surface of the needles.
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:49:06Z
      DOI: 10.1177/09645284221085576
       
  • Health professionals’ attitudes towards acupuncture/acupressure for
           post-operative nausea and vomiting: a survey and implications for
           implementation

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      Authors: Zhen Zheng, Wanda S Stelmach, Jason Ma, Juris Briedis, Raphael Hau, Mark Tacey, Jeannette Atme, Debra Bourne, Julie Crabbe, Catherine Fletcher, Paul Howat, Jenny Layton, Charlie C Xue
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Purpose:Level 1 evidence supports the use of acupuncture/acupressure (A/A) to manage post-operative nausea and vomiting (PONV). This study aimed to survey healthcare professionals’ attitudes towards A/A, influencing factors and barriers to implementing this effective non-drug intervention into peri-operative care.Methods:A validated, anonymous survey with 43 questions was emailed or distributed as a hard copy at meetings to anaesthetists, midwives, nurses, obstetricians, gynaecologists and surgeons at a public hospital in Australia. Descriptive data were presented. Influencing factors were explored using chi-square analysis. Multinomial logistical regression was used to identify the influences of confounding factors.Results:A total of 155 completed surveys were returned, reflecting a response rate of 32%. The majority of participants were female (69%), nurses/midwives (61%) and aged between 20 and 50 years old (76%). Eighty-three percent of respondents considered A/A ‘clearly alternative’ medicine or ‘neither mainstream nor alternative’. Eighty-one percent would encourage patients to use acupressure for PONV if it was offered at the hospital. Previous personal use of A/A was the key factor influencing attitudes and openness to clinical use. The key barriers to implementation were perceived lack of evidence and lack of qualified providers and time.Conclusion:Hospital-based healthcare professionals strongly supported the evidence-based use of A/A for PONV despite considering the therapy to be non-mainstream and having limited A/A education or history of personal use, providing a positive context for an acupressure implementation study. Significant gaps in training and a desire to learn were identified.
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:46:33Z
      DOI: 10.1177/09645284221085282
       
  • Antidepressant effects of acupuncture in a murine model: regulation of
           neurotrophic factors

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      Authors: Teruhisa Yamamoto, Jun Kawanokuchi, Nobuyuki Nagaoka, Ken Takagi, Torao Ishida, Tomoya Hayashi, Ning Ma
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:GV20 and Yintang are important targets in acupuncture treatment for depression. In this study, we examined the antidepressant effects of simultaneous acupuncture stimulation at GV20 and Yintang.Methods:We compared the antidepressant effects of manual acupuncture (MA) stimulation at GV20 and Yintang, compared to acupuncture stimulation at two control point locations on the back of the mice (overlying the spinal column) and imipramine administration in a forced swimming (FS)-induced mouse model of depression, and examined the mRNA and protein expression of neurotrophic factors, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin (NT)-3, and NT-4/5 in the brains by real-time polymerase chain reaction in two different experimental schedules – preventive (MA given alongside FS modelling) and therapeutic (MA given after FS-induced depression was already established).Results:MA at GV20 and Yintang significantly reduced the immobility time of mice with FS-induced depression in both preventive and therapeutic experimental designs, with effects that were comparable to those of imipramine administration. Immobility time following simultaneous acupuncture stimulation of the two control point locations overlying the spinal column was significantly suppressed only 2 weeks after the start of FS in the preventive effect experiment, and the suppressive effect was significantly lower than that of simultaneous acupuncture stimulation at GV20 and Yintang. In the therapeutic effect experiment, there was no change in the increase in immobility time after the end of FS. MA at GV20 and Yintang significantly increased the expression of BDNF and NT-3 in the preventive evaluation and NGF, BDNF, NT-3, and NT-4/5 in the therapeutic effect evaluation.Conclusion:Our findings suggest that simultaneous acupuncture stimulation at GV20 and Yintang is effective for the prevention and treatment of depression, and the effect likely involves modulation of the expression of multiple neurotrophic factors.
      Citation: Acupuncture in Medicine
      PubDate: 2022-05-17T08:43:40Z
      DOI: 10.1177/09645284221085279
       
  • Electroacupuncture attenuates ac4C modification of P16 mRNA in the ovarian
           granulosa cells of a mouse model premature ovarian failure

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      Authors: Zixiang Geng, Peng Liu, Jiajia Lin, Xiaoli Nie, Long Yuan, Kaiyong Zhang, Huiru Jiang, Bingrong Li, Te Liu, Bimeng Zhang
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Premature ovarian failure (POF) is a type of pathological aging, which seriously interferes with the fertility of affected women. Electroacupuncture (EA) may have a beneficial effect; however, its mechanism of action is unknown. The purpose of this study was to determine the effect of EA on ovarian function in ovarian granulosa cells (OGCs) in a cyclophosphamide (CTX)-induced mouse model of POF.Methods:Mice were divided into three groups: wild type (WT) group, CTX group and CTX + EA group. EA was administered under isoflurane anesthesia at CV4, ST36 and SP6 for 30 min every 2 days, 2–3 times per week for a total of 4 weeks. Effects of EA on ovarian weight and level of estrogen were examined. The mRNA and protein expression levels of cell cycle–associated proteins were detected and mRNA modifications were analyzed.Results:EA significantly increased ovarian weight and reduced the proportion of atretic follicles in mice with CTX-induced POF (p < 0.05). EA increased the level of estrogen in the peripheral blood of mice and inhibited the modification of total mRNA N4-acetylcytidine (ac4C). A significant increase in the expression of P16 and N-acetyltransferase 10 (NAT10) and a significant decrease in the expression of Cyclin D (CCND1) and cyclin-dependent kinase 6 (CDK6) were observed in the OGCs of POF mice (p
      Citation: Acupuncture in Medicine
      PubDate: 2022-04-27T12:14:18Z
      DOI: 10.1177/09645284221085284
       
  • Acupuncture in addition to usual care for patients with irritable bowel
           syndrome: a component network meta-analysis

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      Authors: Yu Gan, Shi-Le Huang, Meng-Qi Luo, Min Chen, Hui Zheng
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:The efficacy of acupuncture alone in the treatment of irritable bowel syndrome (IBS) is controversial, but the benefit of acupuncture added to usual care has rarely been studied. We aimed to examine the benefit of acupuncture added to usual care through network meta-analysis (NMA).Methods:PubMed, Embase, and the Cochrane Register of Controlled Trials (CENTRAL) were searched from their inception to 1 July 2021, without any language restriction. Randomized controlled trials (RCTs) testing the effect of acupuncture alone or acupuncture combined with usual care for IBS were included. The primary outcome was improvement of global IBS symptoms. Standard NMA was performed to compare differential combinations of acupuncture (including manual acupuncture (MA) and electroacupuncture (EA)), and component network meta-analysis (CNMA) was subsequently performed to determine whether acupuncture provided additional benefits to usual care. The effect size of an intervention was measured using relative ratio (RR).Results:We included 25 RCTs (n = 3041 participants) after screening 582 retrieved articles. Five RCTs were classified as low risk of bias. The results of standard NMA showed that MA combined with usual care ranked the most effective (sham acupuncture as common comparator; RR = 1.96 (95% confidence interval (CI) 1.23 to 3.12)). The results of CNMA showed that MA was the most effective component (RR = 1.38 (95% CI, 1.12 to 1.70)) when added to usual care.Conclusion:Acupuncture provided additional benefits to usual care, and it might be considered as adjunctive therapy for patients who respond inadequately to usual care.
      Citation: Acupuncture in Medicine
      PubDate: 2022-04-19T06:57:22Z
      DOI: 10.1177/09645284221085280
       
  • Electroacupuncture reduces weight, skinfold thickness and waist
           circumference and increases skin temperature of the abdominal region in
           women: a randomized controlled trial

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      Authors: Isabella Gomes de Lima, Josie Resende Torres da Silva, Amanda Godoy da Silva, Alice de Sousa Andrade, Ligia de Sousa
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Acupuncture, as a complementary and alternative medical treatment, has shown some promise as a therapeutic option for obesity and weight control. The aim of the current study was to investigate the effects of electroacupuncture (EA) on body weight, body mass index (BMI), skin fold thickness, waist circumference and skin temperature of the abdominal region in non-obese women with excessive abdominal subcutaneous fat.Methods:A total of 50 women with excessive abdominal subcutaneous fat (and average BMI of 22) were randomly assigned to one of two groups: an EA group (n = 25) receiving 10 EA sessions (insertion of needles connected to an electrical stimulator at a frequency of 40 Hz for 40 min) and a control group (n = 25) that received no treatment. Outcome measures evaluated included waist circumference, supra-iliac and abdominal skinfolds, body composition and superficial skin temperature (measured by cutaneous thermography) before and after treatment.Results:Compared with the untreated group, women in the EA group exhibited decreased supra-iliac and abdominal skin folds (p < 0.001), waist circumference (p < 0.001), percentage body fat (p = 0.001) and percentage abdominal fat (p < 0.001). In addition, the EA group showed an elevated skin temperature at the site of the treatment. However, EA did not significantly impact body weight (p = 0.01) or BMI (p = 0.2).Conclusion:EA promoted a reduction in abdominal waist circumference, supra-iliac and abdominal skin folds, and percentage body and abdominal fat in women of normal BMI with excessive abdominal subcutaneous fat, as well as an increase in the superficial skin temperature of the abdominal region.Trial registration number:RBR-9tsmpp (Brazilian Registry of Clinical Trials).
      Citation: Acupuncture in Medicine
      PubDate: 2022-04-19T06:55:22Z
      DOI: 10.1177/09645284221077111
       
  • Efficacy of acupuncture in improving symptoms and quality of life of
           patients with acne vulgaris: a randomized sham acupuncture-controlled
           trial

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      Authors: Ruimin Jiao, Xu Zhai, Xuecheng Zhang, Zhiyi Xiong, Zhishun Liu
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:The aim of this study was to examine the effectiveness of acupuncture in treating the symptoms and quality of life (QoL) of patients with moderate or severe acne vulgaris (AV).Methods:Participants were randomly assigned (1:1) to receive 12 treatment sessions of acupuncture or sham acupuncture over 4 weeks with 24 weeks of follow-up. The primary outcome was the change from baseline in the Skindex-16 scale total score at treatment completion. Secondary outcomes included Skindex-16 subscale score, Dermatology Life Quality Index scale total score, total lesion count and inflammatory lesion count, and visual analogue scale scores for itch and pain evaluation.Results:There was no statistically significant between-group difference for the primary outcome or any secondary outcomes after 4 weeks of treatment and at 16 and 28 weeks of follow-up, except for the Skindex-16 emotions subscale at week 4 (p = 0.026). No serious adverse events occurred in either group.Conclusion:Acupuncture may not effectively relieve the symptoms of patients with moderate or severe AV, or improve QoL. Given the limitations of a relatively short treatment course compared to other studies and the likelihood that sham acupuncture is not inert, further studies with treatment durations of 12 weeks or longer and a waitlist (no treatment) control or Western medicine-treated control group should be considered to evaluate the effects of acupuncture on AV.Trial registration number:ChiCTR-1900023649 (Chinese Clinical Trial Registry)
      Citation: Acupuncture in Medicine
      PubDate: 2022-04-19T06:51:26Z
      DOI: 10.1177/09645284221076506
       
  • Acupuncture for insomnia after ischemic stroke: an assessor-participant
           blinded, randomized controlled trial

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      Authors: Yan Cao, Yin-Jie Yan, Jian-Yang Xu, Abulikemu Liwayiding, Yi-Ping Liu, Xuan Yin, Li-Xing Lao, Zhang-Jin Zhang, Shi-Fen Xu
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:To date, there has been little focus on research into acupuncture for insomnia after ischemic stroke. Insomnia is one of the most common sequelae after ischemic stroke, and it is the most unrecognized modifiable risk factor.Objective:To evaluate the efficacy and safety of acupuncture for insomnia after ischemic stroke.Methods:In this assessor-participant blinded, randomized, controlled trial, 144 ischemic stroke patients with insomnia meeting Diagnostic and Statistical Manual of Mental Disorders (fifth edition, DSM-5) criteria were assigned to verum or sham acupuncture treatment (n = 72 per group) for three sessions per week over 4 weeks. The outcomes were the Insomnia Severity Index (ISI), Pittsburgh Sleep Quality Index (PSQI), stroke-specific quality of life (SSQoL), and Hospital Anxiety and Depression Scale (HADS) scores. Multiple objective sleep variables were recorded using actigraphy. Assessment was conducted at baseline, and thereafter once biweekly for the 4-week treatment and at 4 weeks of follow-up.Results:The verum acupuncture group had significantly greater improvements than the sham acupuncture group in sleep quality from 2 weeks into treatment throughout the follow-up, indicated by ISI scores and actigraphic variable SE (sleep efficiency). This greater improvement was also observed in the PSQI after 4 weeks of treatment throughout follow-up, as well as actigraphic variable TST (total sleep time), SSQoL and HADS scores at the end of treatment, and SSQoL and depression scores at follow-up. There was no significant difference between groups in the actigraphic variable SA (sleep awakenings). Adverse events were mild in severity, and their incidence was not significantly different between the two groups.Conclusion:Acupuncture appears to be efficacious, in terms of improving insomnia, related quality of life, and affective symptoms, for patients with ischemic stroke.Trial registration number:ChiCTR-IIC-16008382 (Chinese Clinical Trial Registry).
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-23T05:50:04Z
      DOI: 10.1177/09645284221077106
       
  • Efficacy and safety of electroacupuncture for post-stroke depression: a
           randomized controlled trial

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      Authors: Wa Cai, Wen Ma, Yi-Jing Li, Guan-Tao Wang, Hong Yang, Wei-Dong Shen
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:The objective of this study is to evaluate the efficacy and safety of electroacupuncture (EA) treatment for post-stroke depression (PSD).Methods:This study was a single-center, single-blinded, parallel-arm randomized controlled trial. In total, 65 patients with PSD were randomly allocated into EA and sham EA groups. Treatment was administered at GV20, Sishencong, SP6, LR3 and BL18 in both groups. The EA group received EA treatment, while the sham EA group received sham EA treatment using the Park device. Treatment was given three times a week for 4 weeks. The primary outcome was the Hamilton Rating Scale for Depression (HRSD). Secondary outcomes included the Zung Self-Rating Depression Scale (SDS), National Institutes of Health Stroke Scale (NIHSS), Barthel Daily Living Index (BI) and depression scale of traditional Chinese medicine (TCM). Primary and secondary outcomes were assessed at baseline, week 2 after treatment, week 4 after treatment and week 8 of follow-up. Safety assessment was conducted at each visit for 4 weeks of treatment.Results:Significant differences in HRSD, SDS, NIHSS, BI and TCM scale scores were found in the EA group before and after acupuncture treatment (all p < 0.001). Compared with the sham EA group, HRSD scores improved significantly in the EA group at the end of week 2 (F = 31.33, p < 0.001), week 4 (F = 35.58, p < 0.001) and week 8 after treatment onset (F = 25.03, p < 0.001). Similarly, significant improvements were observed in SDS, NIHSS and BI scores. Two participants in the EA group suffered a local hematoma, while no adverse events were reported in the sham EA group.Conclusion:EA appears to be an efficacious and safe treatment for PSD. According to our results, EA may alleviate depressive symptoms, and improve neurological function and capabilities with respect to activities of daily living (ADLs).Trial registration number:ChiCTR-IOR-17012610 (Chinese Clinical Trial Registry).
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-02T09:59:10Z
      DOI: 10.1177/09645284221077104
       
  • Effects of electroacupuncture on DNA methylation of the TREM2 gene in
           senescence-accelerated mouse prone 8 mice

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      Authors: Jing Jiang, Hao Liu, Zidong Wang, Huiling Tian, Shun Wang, Jiayi Yang, Zhigang Li
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:To explore the mechanism by which electroacupuncture (EA) upregulates triggering receptor expressed on myeloid cells 2 (TREM2) protein in the hippocampus of Alzheimer’s disease (AD) model animals from the perspective of TREM2 DNA methylation.Methods:In total, 24 eight-month-old senescence-accelerated mouse prone 8 (SAMP8) mice were divided into an (untreated) AD group (n = 8), donepezil group (receiving donepezil treatment, n = 8) or EA group (receiving an EA intervention, n = 8). A healthy control group comprising 8-month-old senescence-accelerated mouse resistant 1 (SAMR1) mice (n = 8) was also included. Western blotting, bisulfite sequencing, and oxidative bisulfite sequencing were applied to test the relative expression of TREM2 protein and the methylation levels of the TREM2 gene.Results:EA significantly upregulated the relative expression of TREM2 protein (p < 0.01), downregulated the 5-methylcytosine level (p < 0.01) and upregulated the 5-hydroxymethylcytosine level (p < 0.05) in the hippocampus.Conclusion:Downregulation of 5-methylcytosine levels and upregulation of 5-hydroxymethylcytosine levels in the TREM2 gene might be the mechanism by which EA promotes the expression of TREM2 protein.
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-02T09:54:54Z
      DOI: 10.1177/09645284221077103
       
  • Preoperative electroacupuncture for postoperative nausea and vomiting in
           laparoscopic gynecological surgery: a randomized controlled trial

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      Authors: Juan Zhu, Sha Li, Wenzhong Wu, Jie Guo, Xiaoqiu Wang, Guang Yang, Zhigang Lu, Fangbing Ji, Rong Zou, Zhen Zheng, Man Zheng
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:We aimed to evaluate the effectiveness and safety of preoperative electroacupuncture (EA) on the incidence of postoperative nausea and vomiting (PONV), and severity of postoperative pain, in gynecological patients undergoing laparoscopic surgery. The effects of EA administered at different preoperative time points were compared.Methods:A total of 413 patients undergoing elective laparoscopic gynecological surgery were randomly allocated into 4 groups receiving EA the day before surgery (Group Pre, n = 103), 30 min before (Group 30, n = 104) or both (Group Comb, n = 103), or usual care alone (Group Usual, n = 103). All acupuncture groups had usual care. The incidence of PONV and pain at 24 h were primary outcomes. Secondary outcomes included the severity of postoperative nausea, vomiting and pain, requirement for antiemetic medication and quality of recovery (QoR)-15 scores after surgery.Results:There were significant differences between the four groups in nausea and vomiting incidence (0–24 h), postoperative antiemetic use (0–48 h), and postoperative pain (0–6 h), with the EA groups recording the lowest levels. Regarding primary outcomes, incidence of nausea and vomiting at 6-24 h was 28/11/18/11% (p = 0.003) 23/5/8/9% (p < 0.001), respectively, for Groups Usual/Pre/30/Comb. Accordingly, EA reduced the incidence of nausea and vomiting at 6-24 h by 61/34/60% and 79/65/61% for Groups Pre/30/Comb, respectively. Regarding secondary outcomes, incidence of nausea and vomiting at 0-6 h was 20/9/11/7% (p = 0.013) and 17/7/9/6% (p = 0.021), respectively, for Groups Usual/Pre/30/Comb. Rescue antiemetics at 0–6 h were required by 18/4/11/4% (p = 0.001) in Groups Usual/Pre/30/Comb. The mean numerical rating scale (NRS) pain score (0–10) at 0–6 h was significantly different between groups (2.45/1.89/2.01/1.97 for Groups Usual/Pre/30/Comb, p = 0.024). There were no significant differences between the three EA-treated groups.Conclusion:In gynecological patients undergoing laparoscopic surgery and treated with multimodal antiemetic methods, one session of preoperative EA may be a safe adjunctive treatment for PONV prophylaxis. Optimal timing of EA requires further verification.Trial registration number:ChiCTR-INR-16010035 (Chinese Clinical Trial Registry).
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-01T11:45:44Z
      DOI: 10.1177/09645284221076517
       
  • Electroacupuncture ameliorates neuroinflammation in animal models

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      Authors: Yue-yang Xin, Jin-xu Wang, Ai-jun Xu
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Neuroinflammation refers to a wide range of immune responses occurring in the brain or spinal cord. It is closely related to a variety of neurodegenerative diseases, for which it potentially represents a new direction for treatment. Electroacupuncture (EA) is one method of acupuncture treatment, which can be used as an adjuvant therapy for many diseases. This review focuses on molecular mechanisms of EA in the reduction of neuroinflammation, summarizes relevant basic research and outlines future directions for investigation.Findings:A growing body of basic research has shown that EA can ameliorate neuroinflammation centrally (in animal models of ischemic stroke, Alzheimer’s disease, traumatic brain injury, spinal cord injury, Parkinson’s disease and vascular dementia) and peripherally (e.g. after a surgical insult or injection of lipopolysaccharide) and that its effects involve different molecular mechanisms, including activation of the α7 nicotinic acetylcholine receptor signaling pathway and P2 type purinergic receptors, inhibition of nuclear factor κB, and mitigation of damage secondary to oxidative stress and NOD-like receptor protein 3 inflammasome activation.Conclusions:EA is capable of regulating multiple cell signal transduction pathways to alleviate neuroinflammation in animal models. Although the findings of animal studies are encouraging, further prospective clinical trials are needed to verify the efficacy of EA for the treatment of neuroinflammation.
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-01T11:37:41Z
      DOI: 10.1177/09645284221076515
       
  • Acupuncture intervention for acute pain in the Emergency Department trial:
           a consensus process

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      Authors: Arya Nielsen, Juli Olson, Megan Quesada, Chongbin Zhu, Erin Raskin, Bobbee Vang, Jeannette Painovich, Megan Scott, Vashir J Xiong, Jeffery A Dusek
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Purpose:This document describes the consensus process and intervention for a National Institutes of Health (NIH)-funded multi-site feasibility study utilizing acupuncture for ACUte paIn in The EmergencY Department (ACUITY). The acupuncture intervention is designed to be flexible and responsive to the most common Emergency Department (ED) scenarios, including trauma, acute pain of the low back, abdomen and/or musculoskeletal system, renal colic and headache.Background:Opioids remain a primary treatment for acute ED pain with attendant risk of adverse effects, addiction liability, diversion and death. Effective/safer options for acute pain are needed. Although acupuncture therapy has shown promise for acute pain in the ED alone or in conjunction with usual care, pragmatic trials are needed to obtain definitive and generalizable evidence.Methods:An Acupuncture Advisory Panel was convened that included nine acupuncture experts with 5–44 years of experience in practice and 2–16 years of experience in the acute pain care setting. A modified Delphi process was used with provision of a literature review, surveys of our panel members, three online discussions and email discussion as needed. The STandards for Reporting Interventions in Controlled Trials (STRICTA) checklist was used as a guide.Results:A responsive acupuncture intervention was agreed on for ACUITY. Session forms were fashioned in REDCap (Research Electronic Data Capture program to capture essential treatment data, assess fidelity and inform our design for a future pragmatic multi-site randomized controlled trial (RCT) of acupuncture in the ED, and for use by other future researchers.Conclusion:Development of a responsive manualization intervention provides the appropriate framework for conducting a future, pragmatic, multi-site, definitive RCT of acupuncture in the ED.Trial registration number:NCT04880733 (ClinicalTrials.gov).
      Citation: Acupuncture in Medicine
      PubDate: 2022-03-01T11:14:47Z
      DOI: 10.1177/09645284221076507
       
  • Electroacupuncture attenuates pulmonary vascular remodeling in a rat model
           of chronic obstructive pulmonary disease via the VEGF/PI3K/Akt pathway

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      Authors: Lanxi Zhang, Yange Tian, Peng Zhao, Fanli Jin, Yufang Miao, Yang Liu, Jiansheng Li
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Chronic obstructive pulmonary disease (COPD) is characterized by airflow limitation that is not fully reversible. Pulmonary vascular remodeling is the main pathological feature of COPD. Vascular endothelial growth factor (VEGF), the key regulator of angiogenesis, mediates activation of the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway, which regulates the proliferation and migration of vascular endothelial cells and plays important roles in pulmonary angiogenesis and remodeling in COPD. Here, the efficacy of electroacupuncture (EA) with respect to regulation of microvascular remodeling induced by VEGF/PI3K/Akt was evaluated in a rat model of COPD.Methods:Rats were randomly assigned to blank, COPD model, EA and sham acupuncture (SA) groups. Rats in the EA group received EA at GV14, BL13 and BL23 three times per week, while those in the SA group, as a control, received shallow and minimal electrostimulation at sites 5–10 mm away from the traditional acupuncture point locations. After 2, 4 and 8 weeks of treatment, the optimal treatment duration was determined according to the results of lung function, lung pathology and inflammatory factor levels. Then, microvessel density, protein levels and mRNA expression of selected VEGF/PI3K/Akt pathway intermediates were determined by immunofluorescence, immunohistochemistry and Western blot analysis, and mRNA qRT-PCR, respectively.Results:EA improved lung function and lung tissue histopathology, with the best effect after 8 weeks of treatment, as noted by reduced density of lung microvessels and expression of angiogenesis-related factors (VEGF and endothelin (ET)-1). EA-treated COPD rats exhibited reduced VEGF, VEGF receptor 2 (VEGFR2), ET-1 mRNA and VEGF, VEGFR2, phosphorylated (p)-VEGFR2, PI3K, Akt, p-Akt, mammalian target of rapamycin (mTOR), and p-mTOR at the protein level in comparison with untreated and SA-treated COPD model rats.Conclusion:EA had beneficial effects on COPD in this animal model including reduced pulmonary vascular remodeling via mechanisms possibly related to the VEGF/PI3K/Akt pathway.
      Citation: Acupuncture in Medicine
      PubDate: 2022-02-26T06:53:03Z
      DOI: 10.1177/09645284221078873
       
  • Electroacupuncture suppresses spinal nerve ligation-induced neuropathic
           pain via regulation of synaptic plasticity through upregulation of basic
           fibroblast growth factor expression

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      Authors: Kecheng Zhou, Qiaoyun Wu, Jingjing Yue, Xiaolan Yu, Xinwang Ying, Xiaolong Chen, Ye Zhou, Guanhu Yang, Wenzhan Tu, Songhe Jiang
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Improving synaptic plasticity is a good way to alleviate neuropathic pain. Electroacupuncture (EA) is currently used worldwide to treat this disease, but its specific mechanisms of action need further investigation. Evidence has suggested that basic fibroblast growth factor (bFGF) plays an important role in promoting nerve regeneration and can promote the expression of vascular endothelial growth factor (VEGF).Objective:In this study, we examined the effects of EA on synaptic plasticity and its underlying mechanism.Methods:A spinal nerve ligation (SNL) rat model was established. NSC37204 (a specific inhibitor of bFGF) was used to determine the relationship between bFGF and putative EA-mediated improvements in synaptic plasticity. Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were assessed to evaluate hyperalgesia in rats with SNL. Tissue morphology was detected by hematoxylin–eosin (HE) and Nissl staining, while neural plasticity and its molecular mechanisms were examined by Western blotting, quantitative real-time polymerase chain reaction (qPCR), dual-label immunohistochemistry and transmission electron microscopy.Results:We found that EA improved synaptic plasticity, consistent with higher levels of expression of bFGF and VEGF. Contrary to the beneficial effects of EA, NSC37204 promoted synaptic reconstruction. Furthermore, EA-induced improvements in the neurobehavioral state and improved synaptic plasticity were blocked by NSC37204, consistent with lower expression levels of bFGF and VEGF.Conclusion:These findings indicate that EA suppresses SNL-induced neuropathic pain by improving synaptic plasticity via upregulation of bFGF expression.
      Citation: Acupuncture in Medicine
      PubDate: 2022-02-01T09:48:10Z
      DOI: 10.1177/09645284211066499
       
  • Electroacupuncture alleviates anxiety and modulates amygdala CRH/CRHR1
           signaling in single prolonged stress mice

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      Authors: Jing Zhu, Chang Wang, Yu wang, Chunxia Guo, Pingping Lu, Fangfang Mou, Shuijin Shao
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Post-traumatic stress disorder (PTSD) is an anxiety-related psychiatric disorder, manifesting high comorbidity with anxiety disorders. Its underlying neurobiological mechanisms have been associated with hypothalamic–pituitary–adrenal (HPA) axis dysfunction and stress hormones. Corticotropin-releasing hormone (CRH) is a primary stress hormone, expressed in the hypothalamus and amygdala. Electroacupuncture (EA) can improve mood disorders, but its mechanisms have not been fully elucidated. The aim of this study was to observe the effect of EA on PTSD and explore the related mechanisms.Methods:We used single prolonged stress (SPS) mice to establish a PTSD model, and EA was performed after SPS or 7 days later for a week. Then we observed their fear and anxiety-like behavior through cue-induced fear condition tests, open field test and the elevated zero maze. CRH and CRH receptor 1 (CRHR1) protein levels in the amygdala were measured in SPS mice after EA intervention.Results:We found that EA at ST36 and GV20 improved fear and anxiety behavior in SPS mice. The amygdala CRH and CRHR1 protein levels increased in the SPS mice, and this effect was reversed by the EA intervention. CRHR1 inhibition by the CRHR1 antagonist NBI 27914 alleviated anxiety behavior in SPS mice.Conclusion:CRH/CRHR1 signaling in the amygdala may contribute to the anxiolytic effect of EA in SPS mice.
      Citation: Acupuncture in Medicine
      PubDate: 2022-01-19T06:19:08Z
      DOI: 10.1177/09645284211056352
       
  • Low-frequency electroacupuncture improves disordered hepatic energy
           metabolism in insulin-resistant Zucker diabetic fatty rats via the
           AMPK/mTORC1/p70S6K signaling pathway

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      Authors: Xiao-xiao Liu, Li-zhi Zhang, Hai-hua Zhang, Lan-feng Lai, Yi-qiao Wang, Jian Sun, Neng-gui Xu, Zhi-xing Li
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background and aim:Disordered hepatic energy metabolism is found in obese rats with insulin resistance (IR). There are insufficient experimental studies of electroacupuncture (EA) for IR and type 2 diabetes mellitus (T2DM). The aim of this study was to probe the effect of EA on disordered hepatic energy metabolism and the adenosine monophosphate (AMP)-activated protein kinase (AMPK)/mammalian target of rapamycin complex 1 (mTORC1)/ribosomal protein S6 kinase, 70-kDa (p70S6K) signaling pathway.Methods:Zucker Diabetic Fatty (ZDF) rats were randomly divided into three groups: EA group receiving EA treatment; Pi group receiving pioglitazone gavage; and ZF group remaining untreated (n = 8 per group). Inbred non-insulin-resistant Zucker lean rats formed an (untreated) healthy control group (ZL, n = 8). Fasting plasma glucose (FPG), fasting insulin (FINS), C-peptide, C-reactive protein (CRP) and homeostatic model assessment of insulin resistance (HOMA-IR) indices were measured. Hematoxylin–eosin (H&E) staining was used to investigate the liver morphologically. The mitochondrial structure of hepatocytes was observed by transmission electron microscopy (TEM). Western blotting was adopted to determine protein expression of insulin receptor substrate 1 (IRS-1), mTOR, mTORC1, AMPK, tuberous sclerosis 2 (TSC2) and p70S6K, and their phosphorylation. RT-PCR was used to quantify IRS-1, mTOR, mTORC1, AMPK and p70S6K mRNA levels.Results:Compared with the ZF group, FPG, FINS, C-peptide, CRP and HOMA-IR levels were significantly reduced in the EA group (p 
      Citation: Acupuncture in Medicine
      PubDate: 2022-01-17T04:25:04Z
      DOI: 10.1177/09645284211070301
       
  • Application of the revised Cochrane risk of bias tool for acupuncture
           trials: a preliminary comparative analysis of inter-rater reliability

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      Authors: Sae-Rom Jeon, Tae-Hun Kim
      First page: 268
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-02-24T07:17:00Z
      DOI: 10.1177/09645284221076508
       
  • Use of dry needling therapy to improve lower limb dysfunction in a patient
           with 10-year cerebral hemorrhage sequelae: a case report

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      Authors: Li Tang, Shan-Shan Liu, Fang Gao, Qiang-Min Huang, Lin Gan, Hao Ding, Yan Li
      First page: 270
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-02-24T07:12:24Z
      DOI: 10.1177/09645284221076505
       
  • Immediate effect of acupuncture on pelvic floor structure in stress
           urinary incontinence: a case report

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      Authors: Xiuling Song, Zhihao Li, Shuren Ming, Lixin Guan, Xi Wang, Xia Zhang, Lumin Liu, Bingli Chen, Yuelai Chen
      First page: 272
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-02-24T07:22:22Z
      DOI: 10.1177/09645284221076510
       
  • Electroacupuncture for cervicogenic dizziness with somatosensory pulsatile
           tinnitus

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      Authors: Salaheddin Taleb Hessami Azar, Mike Cummings
      First page: 275
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-02-24T07:24:22Z
      DOI: 10.1177/09645284221076514
       
  • Acupuncture as a salvage treatment for sudden sensorineural hearing loss

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      Authors: Min Hee Kim, Jae Young Kwak, Inhwa Choi
      First page: 278
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-02-25T11:07:10Z
      DOI: 10.1177/09645284221076513
       
  • Improvement of prefrontal blood flow in a patient with major depressive
           disorder after acupuncture evaluated by functional near-infrared
           spectroscopy: a case report

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      Authors: Yuto Matsuura, Seiji Hongo, Fumiko Yasuno, Tomomi Sakai
      First page: 281
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-03-01T11:12:56Z
      DOI: 10.1177/09645284221075355
       
  • Acupuncture for hearing loss: a case report

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      Authors: Qunya Dong, Yiting Zhang, Qiqi Wu, Hantong Hu, Hong Gao
      First page: 284
      Abstract: Acupuncture in Medicine, Ahead of Print.

      Citation: Acupuncture in Medicine
      PubDate: 2022-03-01T11:16:34Z
      DOI: 10.1177/09645284221076509
       
  • Effect of acupuncture on menopausal hot flushes and serum hormone levels:
           a systematic review and meta-analysis

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      Authors: Chang Liu, Zhijie Wang, Ting Guo, Lixing Zhuang, Xiao Gao
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:To evaluate the efficacy/effectiveness and safety of acupuncture for the treatment of hot flushes and its impact on serum hormone levels in menopausal women.Methods:A total of 10 databases were searched from their inception to August 2018. Reference lists of reviews and included articles were also hand-searched. Randomized controlled trials (RCTs) comparing the effect of acupuncture versus sham acupuncture, or acupuncture versus hormone therapy (HT), as treatment for menopausal hot flushes were included. Outcomes included hot flush frequency, hot flush severity and serum hormone levels of estradiol (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Meta-analyses were performed using Review Manager 5.3 software.Results:Thirteen RCTs including 1784 patients were selected, seven of which were available for meta-analysis. Compared with sham acupuncture, acupuncture significantly decreased hot flush frequency (mean difference (MD) −0.84, 95% confidence interval (CI) [−1.64, −0.05], I2 = 54%) from baseline to the end of study, but did not impact end scores of hot flush frequency (MD 0.19, 95% CI [−0.61, 0.99], I2 = 0%) or severity (MD 0.02, 95% CI [−0.13, 0.17], I2 = 0%). No differences were found between acupuncture and HT in serum levels of E2 (MD 6.56, 95% CI [−3.77, 16.89], I2 = 76%), FSH (MD 1.06, 95% CI [−1.44, 3.56], I2 = 0%) or LH (MD −3.36, 95% CI [−13.37, 6.65], I2 = 89%).Conclusion:Acupuncture may not decrease hot flush frequency, but yet appears to have similar effects on serum hormone levels as HT, that is, increased E2 and decreased FSH and LH. Considering that no firm conclusions could be drawn due to the low quality and limited number of included trials included, further high-quality RCTs need to be conducted.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-13T10:11:47Z
      DOI: 10.1177/09645284211056655
       
  • Effects of dry needling on function, hypertonia and quality of life in
           chronic stroke: a randomized clinical trial

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      Authors: Sandra Calvo, Natalia Brandín-de la Cruz, Carolina Jiménez-Sánchez, Elisabeth Bravo-Esteban, Pablo Herrero
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Persons with stroke commonly have impairments associated with a reduction in functionality. Motor impairments are the most prevalent, causing an impact on activities of daily life.Objective:The aim of this study was to evaluate the effect of a session of dry needling (DN) applied to the upper extremity muscles on the sensorimotor function, hypertonia, and quality of life of persons with chronic stroke.Methods:A randomized, sham-controlled clinical trial was performed. Participants were randomly assigned into an intervention group that received a single session DN in the biceps brachii, brachialis, flexor digitorum superficialis and profundus, extensor digitorum, adductor pollicis and triceps brachii muscles, or into a control group that received the same treatment but with a sham DN intervention. Treatment outcomes included the Fugl–Meyer Assessment Scale for the upper extremity, the Modified Modified Ashworth Scale, and the EuroQol-5D questionnaire. Measurements were carried out before, immediately after, and 14 days after intervention.Results:Twenty-three persons participated in the study. Significant differences between groups were observed after the intervention in the total wrist–hand motor score (p = 0.023) and sensorimotor score (p = 0.022), for hypertonia in the elbow extensors both after treatment (p = 0.002) and at follow-up (p = 0.018), and in quality of life at follow-up (p = 0.030).Conclusions:A single session of DN improved total wrist–hand motor function and total sensorimotor function in persons with chronic stroke immediately after treatment, as well as quality of life 2 weeks after treatment.Trial registration number:NCT03546517 (ClinicalTrials.gov)
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-13T10:06:01Z
      DOI: 10.1177/09645284211056347
       
  • The importance of the local twitch response during needling interventions
           in spinal pain associated with myofascial trigger points: a systematic
           review and meta-analysis

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      Authors: César Fernández-de-las-Peñas, Gustavo Plaza-Manzano, Jorge Sanchez-Infante, Guido F Gómez-Chiguano, Joshua A Cleland, José L Arias-Buría, Marcos J Navarro-Santana
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:To compare the clinical effects of needling interventions eliciting local twitch responses (LTRs) versus needling without eliciting LTRs when applied to muscle trigger points (TrPs) associated with spinal pain of musculoskeletal origin.Databases and data treatment:Electronic databases were searched for randomized or non-randomized clinical trials where one group received needling intervention where LTRs were elicited and was compared with another group receiving the same intervention without elicitation of LTRs in spinal pain disorders associated with TrPs. Outcomes included pain intensity, pain-related disability, and pressure pain thresholds. The risk of bias (RoB) was assessed using the Cochrane risk of bias tool or ROBINS-I tool, methodological quality was assessed with the PEDro score, and quality of evidence was evaluated using the GRADE approach.Results:Six trials were included. The application of a needling intervention eliciting LTRs was associated with a significant reduction in pain intensity immediately after treatment (mean difference (MD): −2.03 points, 95% confidence interval (CI): −3.77 to −0.29; standardized MD (SMD): −1.35, 95% CI: −2.32 to −0.38, p = 0.02) when compared to the same needling intervention without elicitation of LTRs. No effect at short-term follow-up (MD: −0.20 points, 95% CI: −1.46 to 1.06, p = 0.75) was observed. No significant differences based on elicitation or non-elicitation of LTRs were found in related disability (SMD: −0.05, 95% CI: −0.41 to 0.30, p = 0.77) or pressure pain thresholds (MD: 23.39 kPa, 95% CI: −13.68 to 60.47, p = 0.22).Discussion:Low-level evidence suggests an immediate effect of obtaining LTRs during needling interventions on pain intensity, with no significant effects on related disability or pressure pain sensitivity in spinal pain disorders associated with muscle TrPs.Registration number:OSF Registry—https://doi.org/10.17605/OSF.IO/5ZX9N
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-13T10:04:26Z
      DOI: 10.1177/09645284211056346
       
  • Ear acupuncture as an adjunct in a treatment protocol for anorexia
           nervosa: utilization rate and nurses’ experience

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      Authors: Kajsa Landgren
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Anorexia nervosa (AN) is a life-threatening illness. Sometimes long inpatient treatment is necessary, increasing the anxiety that comes with hospitalization and the necessary weight gain. Safe, non-pharmacological adjunctive therapies that improve subjective health are called for.Objective:The aim of this non-randomized, mixed-methods observational study was to describe the utilization rate and nurses’ experiences of ear acupuncture in a highly specialized clinic for eating disorders in Sweden, in which acupuncture had been implemented as part of routine clinical care. Twenty-five patients with AN-treated voluntarily or by law were included. The semi-standardized National Acupuncture Detoxification Association (NADA) ear acupuncture protocol, sometimes combined with needling at 2 traditional acupuncture point locations on the body, had been implemented as a voluntary adjunct to usual care, twice weekly. To evaluate the acceptance of acupuncture, the study examined how often patients chose acupuncture when offered on schedule, and how often they asked for extra acupuncture sessions. Patients rated their subjective health using the hospital anxiety and depression scale (HADS) and visual analogue scale (VAS), treatment satisfaction with usual care and acupuncture, and health-related quality of life with the RAND36 instrument. A credibility/expectancy questionnaire (CEQ) was used to measure confidence in treatment. Body mass index (BMI) was calculated to follow the patients’ recovery. Nurses’ experiences of giving acupuncture as a part of routine care were captured in interviews, and analysed with content analysis. Ethical approval was obtained.Results:Despite an initially moderate level of trust in acupuncture, the utilization rate of the scheduled acupuncture was 89% and patients asked for extra acupuncture sessions on 28 occasions. No serious side effects were reported. Nurses’ experiences of providing acupuncture were positive. They were generally enthusiastic, although they reported finding it difficult to organize group treatments and to find time for acupuncture sessions if they were not scheduled.Conclusion:Further research into the effectiveness and costs of acupuncture in psychiatric care is needed. This study provides relevant information for clinicians as well as researchers planning future randomized controlled trials.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T09:15:46Z
      DOI: 10.1177/09645284211056948
       
  • Electroacupuncture improves metabolic and ovarian function in a rat model
           of polycystic ovary syndrome by decreasing white adipose tissue,
           increasing brown adipose tissue, and modulating the gut microbiota

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      Authors: Feifei Zhang, Tong Ma, Xiaoyu Tong, Yanjun Liu, Peng Cui, Xiaoqing Xu, Jiemei Shi, Wei Hu, Wenhan Lu, Zhenle Pei, Minzhen Xu, Xin Li, Congjian Xu, Yi Feng
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Polycystic ovary syndrome (PCOS) affects 8%–15% of reproductive-age women and is associated with reproductive disorders, abdominal obesity, hyperinsulinemia, insulin resistance, type 2 diabetes, and cardiovascular diseases. Acupuncture, as a traditional physical therapy method, could affect various metabolic disorders such as obesity, hyperplasia, gout, and cardiovascular and cerebrovascular diseases in clinical practice. Moreover, electroacupuncture (EA) has been shown to decrease body weight in rats with PCOS; however, the mechanism of weight loss and the relationship between adipose tissue and gut microbiota remain unclear.Objective:To explore the effect and mechanism of EA on white and brown adipose tissues and gut microbiota, and its follow-up effect on reproductive function, in a rat model of PCOS.Methods:Daily EA treatment was administered at ST29 and SP6 in a dihydrotestosterone (DHT)-induced PCOS-like rat model (PCOS + EA group). Effects of EA on in vivo and in vitro adipose volume and weight, organ weight coefficients, body weight, hormonal profiles, and estrous cyclicity were measured, and compared with untreated PCOS model rats (PCOS group) and healthy rats (control group). Microbial DNA was extracted from the fecal samples to analyze group abundance and diversity.Results:EA improved estrous cyclicity, decreased body weight, decreased visceral and subcutaneous fat content, and increased brown adipose tissue weight. EA also normalized serum DHT and progesterone levels and improved glucose tolerance. There were few significant differences in the composition or diversity of the gut microbiota between control, PCOS, and PCOS + EA groups, except for the relative abundances of Tenericutes at the phylum level and Prevotella_9 at the genus level, which were significantly different in the PCOS group before and after EA treatment. Both are important microflora, strongly related to body weight.Conclusion:EA regulated the metabolic disorders and improved reproductive function in this PCOS-like rat model by adjusting visceral fat and brown fat, as well as intestinal flora.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T07:31:37Z
      DOI: 10.1177/09645284211056663
       
  • Teaching self-acupuncture survey report

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      Authors: Catrina Davy, John Hughes
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:People have been teaching self-acupuncture (SA) in the United Kingdom since 1977. More recently, a small body of research has been conducted on SA. However, detailed guidelines on teaching and delivery of SA have so far, to our knowledge, not been developed.Methods:Acupuncturists were invited to participate in an online survey about their experiences of teaching SA. Data from the survey were extracted, analysed using descriptive statistics and translated into SA teaching guidelines.Results:One hundred thirty-eight acupuncturists completed the survey. Nineteen percent of participants had taught SA. The most common symptoms for which acupuncturists taught SA were pain, nausea, anxiety, hot flushes, headaches/migraines and breathlessness. The six most commonly taught traditional acupuncture point locations were LI4, ST36, SP6, PC6, LR3 and TE5. No participants had received reports of adverse effects from their patients.Conclusion:Acupuncturists who teach SA find it to be an acceptable and safe option for patients. The presented data have important implications for guiding the practice of SA.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T07:25:57Z
      DOI: 10.1177/09645284211056658
       
  • A randomized controlled clinical trial comparing different numbers of
           acupuncture sessions for migraine

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      Authors: Thanan Supasiri, Chanchai Jariengprasert, Mayuri Phithaksilp, Puritat Sangtongpanichakul, Suchada Anotayanonth, Tawat Buranatawonsom, Krit Pongpirul
      First page: 215
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Acupuncture has shown benefit in preventing migraine attacks, but there has been no clear recommendation about the number of treatment sessions that should be provided.Objectives:The aim of this study was to examine whether 5 sessions of acupuncture treatment is non-inferior to 10 sessions for migraine.Methods:We performed a multicenter, open-label, randomized, controlled clinical trial across five hospitals in Thailand. Migraine patients were randomly assigned into two groups: treatment with 5 sessions of acupuncture (group A) or 10 sessions of acupuncture (group B). Acupuncture was performed twice a week. We measured the number of migraine days, average pain severity according to a 0–10 numeric pain rating scale (NPRS) and quality of life using the EQ-5D-5L questionnaire, comparing 4 weeks after treatment versus baseline.Results:Of 156 patients, 83 and 73 patients were assigned to groups A and B, respectively. Comparing 4 weeks after treatment with baseline, the mean reduction in the number of headache days in groups A and B was 6.4 (95% confidence interval [CI] 4.8 to 7.9) days and 6.4 (95% CI 4.5 to 8.4) days, respectively (p = 0.97). The mean difference between the reduction in headache days of the two groups was −0.1 (95% CI −2.5 to 2.4) days, which included the pre-specified non-inferiority limit of −1. The mean reduction of NPRS scores in groups A and B was 4.5 (95% CI 3.8 to 5.1) and 3.8 (95% CI 3.1 to 4.5), respectively (p = 0.17). Both groups showed an improvement in quality of life.Conclusion:Both 5 and 10 sessions of acupuncture were associated with apparent benefits in terms of preventing migraine attacks, reducing the severity of the headache and improving quality of life, based on comparisons between baseline and follow-up in both study groups. Although we were unable to demonstrate non-inferiority of 5 sessions versus 10 sessions of acupuncture, the effects in the two groups were not significantly different and the temporal effects appeared to last for at least 1 month.Trial registration number:TCTR20170612002 (Thai Clinical Trials Registry).
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-10T06:43:56Z
      DOI: 10.1177/09645284211056017
       
  • A randomized controlled study of acupuncture for pain relief during first
           trimester surgical termination of pregnancy performed under local
           analgesia

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      Authors: Dorothy Yuet Tao Ng, Alexandra Lo, Emily Wing Sze So, Grace Ching Yin Wong, Raymond Hang Wun Li, Yu Yeuk Wong, Ernest Hung Yu Ng
      First page: 224
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Acupuncture reduces pain levels in many painful conditions. This study compared pain levels during surgical termination of first trimester pregnancy by suction evacuation (SE) under local analgesia with and without the use of acupuncture.Methods:In all, 60 nulliparous women undergoing SE before 10 weeks of gestation were randomly assigned into one of the following three groups in a 1:1:1 ratio according to a computer-generated randomization list. In the control group, women received oral diazepam 5 mg and intramuscular (i.m.) injection of pethidine 30 and 15 min, respectively, prior to SE. In the acupuncture group, women received acupuncture 10 min before SE until the end of SE while oral diazepam 5 mg and i.m. injection of normal saline were given. In the combined group, women received acupuncture in addition to the drugs in the control group. Data from 52 participants were analysed. Pain scores during and after SE, post-operative side-effects and satisfaction levels were compared.Results:The three groups had similar baseline characteristics. The median pain levels during SE differed significantly between the control, acupuncture, and combined groups (80, 50 and 66 mm, respectively, p = 0.03). Pain levels during SE in the acupuncture and combined groups were significantly lower than that of the control group. However, the anxiety scores did not differ between the three groups after SE (p = 0.86).Conclusion:Acupuncture can provide additional benefit in terms of pain relief in women undergoing first trimester termination of pregnancy by SE under local analgesia.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T09:16:46Z
      DOI: 10.1177/09645284211057567
       
  • Electroacupuncture and moxibustion-like stimulation activate the cutaneous
           and systemic hypothalamic–pituitary–adrenal axes in the rat

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      Authors: Xiao-Ning Zhang, Wei He, Hong-Ye Wan, Yang-Shuai Su, Qing-Quan Yu, Yi Wang, Xiang-Hong Jing, Bing Zhu
      First page: 232
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Objective:To determine whether electroacupuncture (EA) or moxibustion-like stimulation (MLS) can affect the cutaneous and/or systemic hypothalamic–pituitary–adrenal (HPA) axes.Methods:Rats were divided into Control, EA, 37°C MLS and 43.5°C MLS groups. EA and MLS were performed at bilateral ST36 or LI4. The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. In addition, levels of CRF, ACTH and corticosterone (CORT) in cutaneous tissue and plasma were determined.Results:Cutaneous expression of CRF, ACTH and GR significantly increased after EA at ST36, while only GR increased after 43.5°C MLS at ST36. The results of EA and MLS at LI4 were in parallel with those at ST36. In plasma, compared with the control group, the level of CORT increased after EA at ST36, while both ACTH and CORT were markedly increased after 43.5°C MLS. For LI4, plasma CRF and CORT increased after EA, while the levels of all three hormones increased following 43.5°C MLS. Notably, compared with the effect of EA, 43.5°C MLS at ST36 produced a more substantial increase in plasma CORT, and 43.5°C MLS at LI4 induced a more dramatic increase in plasma CRF and CORT.Conclusion:Both EA and 43.5°C MLS can activate the cutaneous and systemic HPA axes of the rat. EA tended to activate the local cutaneous HPA, while 43.5°C MLS was more likely to activate the systemic HPA axis.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-10T06:39:36Z
      DOI: 10.1177/09645284211055745
       
  • Dry needling increases antioxidant activity and grip force in a rat model
           of muscle pain

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      Authors: Daniel Tassinari Felber, Rafael Tamborena Malheiros, Victor Novo Tentardini, Andréia Caroline Fernandes Salgueiro, Francisco José Cidral-Filho, Morgana Duarte da Silva
      First page: 241
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Muscle pain syndromes (MPS) are one of the main causes of functional, structural and metabolic problems, being associated with tissue oxidative damage. Although dry needling is widely used in the treatment of MPS, there is little scientific evidence of its efficacy and underlying mechanisms of action.Objectives:To investigate the effects of different dry needling techniques on thermal and mechanical hyperalgesia, locomotor and functional activity, and oxidative stress markers in a rat model of muscle pain.Methods:A total of 48 male Wistar rats underwent injection of the gastrocnemius muscle with control neutral saline (pH 7) and remained untreated (Saline group), or acidic saline (pH 4) and remained untreated (ASA group) or received pregabalin (PG group), deep needling (DN group), superficial needling (SN group) or twitch needling (TN group) with n = 8 rats per group. Mechanical (von Frey test) and thermal hyperalgesia (acetone test), muscle edema (assessed with a caliper), strength and muscle function (grip force evaluation), surface thermography and locomotor and exploratory activities (open field test) were evaluated. The animals were then euthanized, and the gastrocnemius muscle was excised for assessment of oxidative analyses of lipid peroxidation with thiobarbituric acid reactive species (TBA-RS) and total glutathione (GSH) levels.Results:All treatments significantly improved muscle strength and function when compared to the AS group (p < 0.05). Pregabalin reduced locomotor and exploratory activities, while the TN intervention increased the antioxidant response (p < 0.05).Conclusion:Dry needling improved strength, functionality and locomotor activity in a rat model of muscle pain. Twitch needling induced an antioxidant effect.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T09:14:38Z
      DOI: 10.1177/09645284211056941
       
  • Electroacupuncture altered expression of microRNAs in Stat5 knockout obese
           mice

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      Authors: Hao Hong, Hou-Xi Xu, Jian-Zhong Meng, Bing-Mei Zhu
      First page: 249
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Increasing evidence shows that miRNAs contribute to the establishment and development of obesity by affecting many biological and pathological processes, such as adipocyte differentiation, hepatic lipid metabolism, insulin resistance, and neurological regulation of obesity. As a clinical intervention approach, acupuncture has been shown to be effective in the treatment of obesity and other metabolic diseases. Our previous whole genome study in central nervous system (CNS)-specific Stat5 knockout (KNO) obese mice found that electroacupuncture (EA) could reduce body weight and promote white browning.Objective:To clarify the effect of EA on miRNAs and understand how it regulates gene expression.Methods:Twelve-week-old male Stat5NKO mice with body weight 20% greater than that of Stat5fl/fl (control) mice were divided into a Stat5NKO (model) group and EA-treated Stat5NKO + EA group. A cohort of Stat5fl/fl mice of the same age were included as the control group. EA was administered under isoflurane anesthesia at unilateral ST36 and ST44 daily (left and right sides were treated every other day), 6 times per week for a total of 4 weeks. The miRNA profile was generated and miRNA regulatory networks were analyzed in the Stat5 nestin-cre mice before and after EA treatment. Autophagy-related proteins in adipocytes were detected after over-expression of miR27a.Results:EA altered abnormal miRNA expression, including miRNA27a expression, and reduced the autophagy-related proteins ATG5 and ATG12.Conclusion:We found that EA could regulate miRNA27a-mediated autophagy-related proteins and promote white fat browning, which may contribute to weight loss. To our knowledge, this is the first report of miRNAs potentially driving the effect of EA on white fat browning through the autophagy process.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T07:15:01Z
      DOI: 10.1177/09645284211056345
       
  • Electroacupuncture promotes microglial M2 polarization in ischemic stroke
           via annexin A1

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      Authors: Jing Zou, Guo-fu Huang, Qian Xia, Xing Li, Jing Shi, Ning Sun
      First page: 258
      Abstract: Acupuncture in Medicine, Ahead of Print.
      Background:Neuroinflammation is the leading cause of neurological sequelae in ischemic stroke. Recently, we reported that the anti-inflammatory mediator annexin A1 (ANXA1) favored microglial M2 polarization in brain injury. The purpose of this study was to investigate electroacupuncture (EA) treatment and its potentially ANXA1-mediated anti-inflammatory effects in the middle cerebral artery occlusion/reperfusion (MCAO/R) mouse model of stroke.Methods:Treatment with EA consisted of dense-sparse frequencies (alternating 4 Hz sparse waves for 1.5 s and 16 Hz dense waves for 1.5 s) at CV24 and GV26. Intracerebroventricular (ICV) injection of Boc-2 (5 µM) or short hairpin RNA (sh)ANXA1 (2 µL) 3 days before EA was performed to block the effects of ANXA1.Results:EA pretreatment enhanced expression of ANXA1 and its receptor, formyl peptide receptor (FPR), when compared to MCAO/R alone. EA treatment also rescued MCAO/R-induced deficits in neurological performance, and learning and memory, and reduced infarct volume. Double immunofluorescent labeling showed that EA prevented MCAO/R-induced changes in microglial activation and morphology. EA also reduced the release of pro-inflammatory cytokines, such as interleukin (IL)-1β, inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF)-α, while increasing the release of anti-inflammatory cytokines, such as arginase-1 (Arg-1) and brain-derived neurotrophic factor (BDNF). All EA-induced effects were either partially or completely prevented by prior administration of FPR antagonist Boc-2 or shANXA1.Conclusion:The current study provides strong evidence that EA treatment has protective effects against ischemic stroke in the MCAO/R mouse model and that the mechanism likely involves the promotion of M2 polarization in microglia via ANXA1.
      Citation: Acupuncture in Medicine
      PubDate: 2021-12-11T09:17:46Z
      DOI: 10.1177/09645284211057570
       
 
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