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Van Veterinary JournalNumber of Followers: 0
 Open Access journal ISSN (Print) 2149-3359
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- Antioxidant and Antihyperlipidemic Effect of Solanum Nigrum Extract in
Experimental Diabetes Model
Authors: Ahmet Cihat ÖNER; Fatmagül YUR, Mohammed Nooraddin FETHULLAH
Abstract: Diabetes mellitus (DM) is a chronic metabolic non-commu¬nicable disease; it is globally considered the fifth cause of death and it has attained worldwide epidemic pro¬portions. In our study, we aimed to investigate the diabetic effects of Solanum nigrum extract using the control group (C), diabetes group (D), groups given the Solanum nigrum extract (SN) and diabetes group + Solanum nigrum extract (D+SN). Our results observed the biological effectiveness of Solanum nigrum extract on glucose levels, significant increase serum glucose level group (D) (663±21.8 mg/dL) in comparison with C (131±9.8 mg/dL) were recorded. However, there were no significant difference in glucose level between C group (131±9.8 mg/dl) and SN group (196.14±12.1 mg/dL). Moreover, glucose level of D+SN group (484.8±40.0 mg/dL) was significantly higher than C (131±9.8 mg/dl), D (663±21.8 mg/dl) and SN groups (196.14±12.1 mg/dL). Total antioxidant status (TAS) level in D group (1.85±0.15.7) was significant when compared C group (1.28±0.17). Significant differences were observed between D group and D+SN group (1.54±0.07). However, TAS levels showed no significant difference in both SN (1.27±0.10) and D+SN (1.54±0.07) groups in comparison to the control group. Total oxidant status (TOS) level in D group (6.30±1.41) was given significant differences in comparison with control C (3.87±0.34), SN (4.87±0.80) group and D+SN (4.14±0.34) groups. In contrary, there were no significant differences between all of C, SN, D+SN groups. As a result, we can say that the Solanum nigrum plant extract is effective on diabetes, but it cannot lower the glucose level to normal levels, it needs to be investigated in future studies and its effects at different doses by different extraction methods.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Hyperglycemic Effect of Dietary Boron in Rats with Experimental Diabetes
Mellitus Induced by Streptozotocin
Authors: Nur AKMAN ALACABEY; Hulya OZDEMİR, Gökhan OTO
Abstract: In this study, the effect of boric acid (BA) on blood sugar levels, vitamins and minerals in Streptozotocin (STZ)-induced diabetes in rats was investigated. In the study, 48 male Wistar albino rats (200-220 g) were divided into 6 groups, no special treatment was administered to Group1, experimental diabetes was induced by administering STZ (45 mg/kg) through intraperitoneal (IP) injection to other groups. Group 2 diabetes; Group 3 (6 U/kg insulin) insulin; Group 4; 250 ppm, group 5; 500 ppm and group 6 1000 ppm BA mixed with their feed. Blood glucose levels of all groups were quantified from blood taken from the tail vein every week. At the end of study, the rats were sacrificed and their blood was taken. The serum levels of vitamins A, E, and minerals were studied. When compared with other groups, blood glucose levels of groups 4, 5 and 6 were found to be increased (p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- The Distribution of Gastrointestinal System Parasites Detected in Horses
in Aydın
Authors: Selin HACILARLIOĞLU; Metin PEKAĞIRBAŞ
Abstract: In the present study aimed to determine the diversity and prevalence of gastrointestinal parasites in horses grazing on pastures in Aydın province through fecal examination. Fresh fecal samples were collected from a total of 242 horses of different ages, genders, and breeds. The samples were examined using Fülleborn's saturated salt flotation and Benedect sedimentation methods. According to the fecal examinations, 182 animals (75.20%) were assessed as positive for digestive system parasites and based on the obtained findings, singular and mixed infections were detected in 150 and 32 samples, respectively. Among the findings, Strongylid-type eggs were detected in 180 samples (74.38%), Anaplocephalidae spp. in 7 samples (2.89%), Parascaris equorum in 4 samples (1.65%), Dicrocoelium dendriticum in 7 samples (2.89%), Fasciola spp. eggs in 6 samples (2.47%), and Eimeria spp. oocysts in 10 samples (4.13%). The study revealed a high prevalence of pasture-related gastrointestinal infections in horses raised in various districts of Aydın province. Consequently, it is recommended that horse breeders be educated and effective control programs be established to combat these parasites.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- The Effect of Short-Term or Long-Term Progesterone-Based Estrus
Synchronization on Fertility Parameters in Tuj Ewes outside the Breeding
Season
Authors: Erdem TAÇYILDIZ; Buket BOĞA KURU, Mushap KURU
Abstract: The objective of this study was to investigate the impact of estrus synchronization on certain fertility parameters in Tuj ewes during the non-breeding season, using short-term (ST) or long-term (LT) progesterone (P4)-impregnated sponge and equine chorionic gonadotropin (eCG). Forty-eight clinically healthy Tuj ewes were selected and divided into three groups: Group I (G1, n=15), Group II (G2, n=18), and Group III (G3, n=15). G1 and G2 received hormonal treatment with a P4-impregnated sponge inserted into the vagina on day 0, and 500 IU eCG was administered intramuscularly five or ten days later, respectively. The sponges were removed from the vagina seven days after insertion for G1 and 12 days after insertion for G2. G3 did not receive any hormone treatment. All groups were exposed to the ram 24 hours after sponge removal. Estrus was monitored every six hours for five days, and pregnancy was confirmed by transrectal ultrasonography. The estrus rate, estrus onset time, and pregnancy rate were significantly different between G1 and G3 and between G2 and G3 (p0.05). In conclusion, ST or LTP4-impregnated vaginal sponge treatment was equally effective on fertility parameters in Tuj ewes during the non-breeding season.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Chemical Composition and Physical Properties of Milk in Norduz Sheep
Authors: Davut KOCA; Ali Osman TURGUT, Nebi ÇETİN, Sefa ÜNER, Erman GÜLENDAĞ, Bahtiyar KARAGÜLLE
Abstract: The objective of this study was to uncover the milk composition traits of Norduz sheep, which are bred in the Gürpınar district of the Van province, during the mid-term lactation period. The chosen ewes exhibited no signs of clinical or subclinical mastitis. A total of 104 sheep milk samples were meticulously collected to undergo comprehensive milk composition analysis. The chemical and physical attributes of Norduz sheep milk were methodically determined utilizing a milk autoanalyzer. The mean values for various milk constituents were as follows: milk fat (%), 2.48±0.11; solids-not-fat (SNF) (%), 10.76±0.08; milk protein (%), 5.09±0.04; lactose (%), 4.79±0.04; pH, 6.93±0.02; conductivity (mS/cm), 4.41±0.04; freezing point (°C), -0.602±0.80; salt (%), 0.8035±0.66; and density (kg/m3), 1039.73±0.39. Noteworthy negative and statistically significant correlations were observed between milk fat and protein (r=-0.36, p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- A Research on the Online Teaching Experiences of Ankara University
Veterinary Faculty Academics
Authors: Aytaç ÜNSAL ADACA; Pınar AMBARCIOĞLU
Abstract: In this study, it has aimed to determine the perceptions of veterinary faculty academics of Ankara University regarding their online teaching experiences during the Covid-19 pandemic. The study has included 92 academics who provided theoretical and/or practical courses via online teaching in Ankara University Faculty of Veterinary Medicine (AUFVM). The data of the study has collected with a 21-item questionnaire. The questionnaire has basic questions such as the quality of the courses given, the active participation of the students in the courses, experienced technological problems during their education, and whether they consider online teaching effective. Academics in clinical sciences gave negative answers to the question about the sustainability of online teaching at a higher rate than the academics in basic sciences (p=0.016). Only all academics who think online teaching has no disadvantages stated that it provides effective learning (p=0.001). Academics who think online teaching provides effective teaching mostly want to continue online, while those with opposing considerations “generally” prefer to continue online or can “sometimes” continue (p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Investigation of the Effect of Malva Plant (Malvasylvestris L.) on Skin
Fungus in Cattle
Authors: Ertan DOĞAN
Abstract: The purpose of this study is to determine the effect of the Malva sylvestris L. (MS) plant on the cutaneous fungus (Trichophytosis, Ringworm) in cattle. The research was carried out on a total of 20 cattle of 6 to 20 months of different races and genders in the Ardahan region. Animals diagnosed with Trichophytosis were divided as control (n=10) and experimental (n=10) groups. No substances were used in the control group. The extract of MS was sprayed with an atomizer onto the lesions of the experimental group. Spraying was done to cover the area where the lesions were located and wet enough. The procedure was conducted twice daily (morning and evening) and for 15 days. Starting from the pre-application (0th day) until the 36th day at intervals of two days, the lesion diameters of the cattle in the control and the experimental group were measured and recorded. At the end of the monitoring period (36th day), the diameter of the lesion was enlarged (29.20±3.58 mm) in the control group and reduced (6.60±5.16 mm) in the experimental group. This difference between the lesion diameters of the control and the experimental groups was statistically significant (p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Effects of Different Stress Applications on Some Reproductive Hormones,
Sperm Parameters, Lipid Profile, Immunohistochemical and Immunofluorescent
Markers
Authors: Saadet BELHAN; Zübeyir HUYUT, Serkan YİLDİRİM, Sermin ALGÜL
Abstract: The current study assessed how 5 stress protocols applied affected sperm parameters, lipid profile, and some reproductive hormones. Live materials of the study consisted of 50 rats. The number of rats in the groups was equal and they were randomly assigned. Each group consisted of 10 rats. No stress application was conducted in the control group. The rats in the psychological stress group were subjected to a cycle of 4-hour light and 20-hour dark per day. The rats in the physical stress group were deprived of feed and water for two hours per day. In the psychological stress + physical stress group, the psychological and physical stress protocol was conducted. In the first 4 groups, all the applications were conducted for 14 days. A different stress application was applied to the rats in the depression group every day. It was determined that the abnormal sperm rate was high in the stress and depression groups, but the highest rate was in the depression group. In addition, sperm motility and sperm concentration were the lowest in the depression group. While the stress and depression groups had significantly lower serum triglyceride and HDL levels and LH and FSH levels, cholesterol and LDL values were significantly higher. Bax expression and 8 OHdG expression were severe in psychological stress+physical stress group and depression group. When the findings are evaluated collectively; it was determined that stress negatively affected sperm parameters, lipid profile, reproductive hormones, immunofluorescence and immunohistochemical parameters.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Investigation of Escherichia coli O157:H7 and Salmonella Bacteriophages in
Cattle Fecal Sources
Authors: Gökçenur SANİOĞLU GÖLEN; Kadir AKAR
Abstract: S. Typhimurium and E. coli O157:H7 are the most important foodborne pathogens forming bacterial biofilms that contribute to their virulence, antimicrobial resistance, and surface survival, causing severe food poisoning outbreaks worldwide. Bacteriophages are antibacterial agents that are increasingly used to control foodborne pathogens, and they also play a role in the solution against the development of antibiotic resistance. In addition, bacteriophages can be found in wastewater, natural and animal wastes, and foodstuffs. Aim of this study to determine the purification and lytic effects of Salmonella spp. and E. coli specific phages circulating in our country, which can effectively combat common Salmonella spp. and E. coli infections in our country and the world by using samples taken from the cowshed. In this study, 3 S. Typhimurium and 1 E. coli O157:H5 bacteriophages were isolated, and their lytic activities were determined. As a result, it is thought that the lytic activities of S. Typhimurium and E. coli O157:H7 bacteriophages purified from Aksaray province in this study can shed light on the treatment of S. Typhimurium and E. coli O157:H7 infections and prevention studies in the food industry.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Honey Produced in Different Regions of Çankırı Antibacterial,
Antioxidant Properties and Some Chemical Parameters
Authors: Pınar PORTAKAL; Şinasi AŞKAR, Seda ÖZGEN
Abstract: Honey is a food source rich in active components with high nutritional properties. Antibacterial, antioxidant activities and quality criteria of honey vary according to the geographical location, climate, plant flora and environmental conditions. This study aimed to determine antibacterial and antioxidant activities and some chemical parameters of honey from different regions of Çankırı province. A total of 14 honey samples were collected from villages in 7 different districts of Çankırı province. The antibacterial activity of the collected honey samples against extended spectrum beta lactamase producing Escherichia coli and methicillin-resistant Staphylococcus aureus was analyzed by disc diffusion and broth microdilution methods. As a result of antibacterial activity tests, it was determined that all honeys had different minimum inhibitory concentration values between 21.5 mg/mL and 42.5 mg/mL against S.aureus and E.coli bacteria and minimum bactericidal concentration values were 42.5 mg/mL and higher. As a result of the analyses, the total amount of phenolic substances in honey was found in the range of 36-84 μg/mL. Moisture ratios of the honeys were 15.40%-20.80%, electrical conductivity was 0.18-0.34 mS/cm, pH values were 3.4-4.4, diastase number was 17.5, the highest proline amount was 944.42 mg/kg and the average values of color parameters L*, a* and b* were 44.31, 1.42 and 18.92 respectively. As a result, it was determined that the honeys of Çankırı region had antibacterial activity at different concentrations, phenolic content and antioxidant activity were parallel, and the chemical parameters were by the Turkish Food Codex Standards.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Modulation of the Immune System and Hemogram Parameters by Prunus spinosa
in Short-Term Hyperglycemia
Authors: Mehmet Ali TEMİZ; Emine OKUMUŞ
Abstract: Diabetes mellitus (DM) is one of the chronic diseases, relationship increased blood glucose level, that requires urgent global attention due to its prevalence and associated complications. DM leads to oxidative stress that plays an important role in the development of various complications in diabetes by suppressing the immune system. Prunus spinosa is a plant that has been used in the treatment of many diseases from past to present, thanks to its high antioxidant activity. Therefore, the present study aims to research the effect of P. spinosa leaf and flower mixture on immune system during the short-term diabetic condition. In the study, 56 Wistar albino male rats divided into 7 groups, one of which control and others six diabetic groups, were used to determine the effects of P. spinosa on adenosine deaminase (ADA), (xanthine oxidase) XO and myeloperoxidase (MPO) activities in the liver tissues of diabetic rats as well as on hemogram parameters. Two of these groups were given plant extract in different concentrations (25 and 50 mg/kg bw) and the results were compared with insulin, metformin and acarbose groups. The results showed that both doses administered had a modulating effect on the changing hematological parameters caused by diabetes. Treatment groups significantly decreased ADA, XO, and MPO activities compared to diabetic group. The effects of the PSE50 were found to be more effective than all other treatment. These effects of the plant in diabetesmay be due to its therapeutic immunoregulatory potential. As a result, P. spinosa can be a valuable resource as an adjuvant on diabetes.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Evaluation of Total and Lipid-Bound Sialic Acids, Trace and Macro
Elements, and Some Biochemical Parameters in Dogs with Babesiosis
Authors: Ugur OZDEK; Yeter DEĞER, Bekir OĞUZ
Abstract: The present study was designed to investigate the changes at the levels of total sialic acid (TSA), lipid-bound sialic acid (LSA), trace and macroelements, and some biochemical parameters in dogs naturally infected with babesiosis. While babesiosis group consisted of seven dogs which were diagnosed with babesiosis clinically and parasitologically (ELISA), control group consisted of seven healthy dogs. Serum TSA and LSA levels in blood samples were measured spectrophotometrically by Sydow and Katapodis methods, respectively. Some biochemical parameters and macroelement measurements were performed using a modular autoanalyzer device. Trace mineral measurements were performed by ICP-MS technique. Compared to the healthy group, dogs with babesiosis had considerably higher TSA and LSA levels. Serum AST, ALP, LDH and CK enzyme activities and CRP, glucose, globulin, total bilirubin, urea, uric acid, creatinine and BUN levels of the babesiosis group significantly increased, while total protein level significantly decreased. The changes in ALT enzyme activity and triglyceride, cholesterol, HDL, LDL, HDL-CDL and ferritin levels were not statistically significant. Zinc, copper, magnesium, sodium, and potassium levels of the babesiosis group decreased significantly, while iron and chlorine levels increased significantly (p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- The Prevalence of Intestinal Parasites in Tumbler Pigeons Raised in
Kırıkkale
Authors: Sami GÖKPINAR; Ece UYSAL, Beyza Nur PUSAT
Abstract: It was aimed to investigate the prevalence of intestinal parasites in tumbler pigeons reared in Kırıkkale. One hundred and five stool samples were obtained as one sample from each cage reached. The fresh stool samples collected were taken separately in containers with caps and delivered to laboratory within approximately 2 hours. Stool samples were analyzed by Carbolfuchsin, Native-Lugol staining and Fulleborn flotation techniques. Eimeria spp. oocysts were sporulated in 2.5% potassium dichromate and the species were identified. Parasite species were found in 82.9% of the examined pigeon stool. In the study, Heterakis spp., Ascaridia spp., Capillaria spp., Strongyle type eggs and Eimeria spp., and Cryptosporidium spp. oocysts were detected. The number of feces infected with one parasite (32.4%) species was higher than the number of feces infected with two (22.9%), three (19.0%) and four species (8.6%). While helminth+protozoan mixed infections were detected in 40.9%, helminth parasite eggs in 13.3% and protozoan oocysts were found alone in 28.6% of the stools examined. All of the sporulated Eimeria oocysts were identified as E. labbeana. In this study, intestinal parasites were detected at a high rate in pigeons fed for hobby purposes. It has been revealed that animal owners should be informed about the issue, attention should be paid to the cleanliness and hygiene of pigeon cages, and more importance should be given to the diagnosis and treatment of intestinal parasites in these animals in order to reduce the prevalence of parasitic infections in pigeons in the region.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Some Renal Marker Levels in Geriatric Rats
Authors: Yıldıray BASBUGAN; Nazmi YÜKSEK, Ahmet KÖMÜROĞLU, Eda Nur OKMAN, Ugur OZDEK
Abstract: The aim of this study was to determine Neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), cystatin C (Cyc-c), and interleukin-18 (IL-18) levels which are frequently used as kidney biomarkers in geriatric rats and to compare with those in young rats. For this purpose, 12 geriatric Wistar albino rats (30-36 months old) (six males and six females) and 12 (2-3 months old) (six males and six females) Wistar albino rats were used in this study. 24-hour urine samples of all groups were collected, and blood was collected for biochemical analysis under anesthesia. The mean body weight of the geriatric rats was statistically higher than the young rats (p
PubDate: Mon, 27 Nov 2023 00:00:00 +030
- Effect of Marbofloxacin, Diclofenac Sodium and Methylprednisolone on Serum
Cytokine Levels in Systemic Endotoxemia
Authors: Ali ŞAHİN; Ahmet Cihat ÖNER
Abstract: It was aimed to evaluate the effects of using marbofloxacin, diclofenacsodium and methylprednisolone on serum cytokine levels in rats with experimental endotoxemia with LPS. In the study, 186 rats were randomly divided into 5 equal groups after the control group (n: 6) was separated. Blood samples were taken from the control group at 0 hour. LPS (4mg/rat) was administered intraperitoneally (IP) to induce endotoxemia in rats. To treat sepsis developing after endotoxemia, marbofloxacin 100mg/kg, diclofenacsodium 10 mg/kg, methylprednisolone 10 mg/kg were administered by IP route. Serum cytokine values were measured by taking blood samples under thiopental anesthesia at 1, 2, 4, 8, 12 and 24 hours following drug administration. The data obtained in the study showed a great deal of similarity to naturally occurring sepsis. When cytokine levels were examined, it was determined that the application of diclofenacsodium and marbofloxacin alone did not have an effect in the treatment of sepsis, but methylprednisolone application could be effective in case of single or combined application. It is suggested that corticosteroid administration alone or in combination with antibiotics and NSAIDs may be beneficial for increased cytokine levels with sepsis.
PubDate: Mon, 27 Nov 2023 00:00:00 +030
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