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  Subjects -> VETERINARY SCIENCE (Total: 225 journals)
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SVU-International Journal of Veterinary Sciences
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  This is an Open Access Journal Open Access journal
ISSN (Print) 2535-1826 - ISSN (Online) 2535-1877
Published by South Valley University Homepage  [1 journal]
  • Protective Effects of Royal Jelly against Hepatorenal Toxicity Induced by
           Deltamethrin in Male ...

    • Abstract: DDeltamethrin (DM) is one of the most environmental and industrial pollutants that are toxic to humans, animals, fishes, and birds. The most common sources of human and animal exposure to deltamethrin (DM) are polluted water and food. This study was done to evaluate the nephrohepatic toxicity of deltamethrin. Twenty-four male rats were used. The first group was used as a control. The second and third groups were given deltamethrin orally in a dose of 1/10 % of the LD50 equal to 0.6mgkg b w alone plus Royal Jelly (RJ) at a dose of 100 mg/kg/day for two months, respectively. Oral administration of DM-induced biochemical and histopathological alterations. DM toxicity exhibited changes in the liver and kidney function tests manifested by an increase in AST, ALT, urea, uric acid and creatinine with no changes noticed in plasma proteins when compared to the control group. Giving RJ ameliorated the hepatorenal toxicity by causing recovery in both liver and kidney functions in comparison to DM given group. Pathologically, severe degenerative and necrotic changes in livers and kidneys were present in the deltamethrin group, where it improved to moderate to mild lesions with a protective royal gel substance. This study concluded that royal gel substance has been shown to benefit in lower down the side effects and increasing the rate of improvement of injury induced by deltamethrin.
      PubDate: Fri, 16 Sep 2022 22:00:00 +010
  • Effect of Cinnamon and Rosemary Nano-Emulsions against Escherichia coli
           O157:H7 Isolated from ...

    • Abstract: The study was conducted to determine the incidence of E. coli serotypes in beef and chicken shawarma sandwiches, particularly E. coli O157:H7. The antibacterial activity of nano-emulsions (NEs) was evaluated, such as cinnamon and rosemary, against E. coli O157:H7. A total of 100 samples from ready-to-eat beef and chicken meat shawarma sandwiches (50 each) were isolated and identified as E. coli using a Sorbitol MacConkey (SMAC) agar assay. The results were confirmed by serology and polymerase chain reaction PCR using the phoA gene, which is specific for E. coli and the fliCH7 gene, which is specific for E. coli O157:H7. Cinnamon and rosemary NEs were prepared, characterized, and evaluated in vitro to determine the minimum inhibitory concentration (MIC) using a well diffusion method. The incidence of E. coli species isolated from beef and chicken shawarma sandwiches samples was 58% and 10%, respectively. While, E. coli O157:H7 was detected in 6% of beef shawarma sandwiches only. Both cinnamon and rosemary NEs exhibited antimicrobial activity against E. coli O157:H7, and the cinnamon NE was more effective compared with that of rosemary with a mean inhibition zone of 7.67 ± 1.202 mm and 7 ± 0.5774 mm at MIC 0.78% and 3.125%. Further studies are required to detect the safety of effectiveness of natural NEs in the food industry.
      PubDate: Fri, 16 Sep 2022 22:00:00 +010
  • Prevalence and public health hazards of subclinical mastitis in dairy cows

    • Abstract: Subclinical mastitis (SCM) is an asymptomatic udder infection distributed worldwide that causes significant losses in the dairy industry. The study aims to detect the prevalence of this pathological condition and to identify the most prevalent related pathogens. A total of 440 quarter milk samples from 110 dairy cows were subjected to California mastitis test (CMT) and Modified Whiteside test (MWST) to quantify their efficacy in detecting subclinical mastitis in dairy cows. Quarter-wise prevalence of subclinical mastitis (SCM) was detected in 30.23% and 28.64% samples by CMT and MWST, respectively, while animal-wise prevalence of SCM was recorded in 60% and 55.45% by CMT and MWST, respectively. The left and right forequarter were most susceptible to SCM than other quarters. All positive samples by field tests were subjected to microbiological examinations. Staphylococcus aureus (S. aureus) (48.51%) which considered the primary pathogens among the bacterial isolates followed by Coagulase negative Staphylococci (40.09%), Escherichia coli (E.coli) (38.12%) and Streptococcus agalactiae ( S.agalactiae) (13.37%). The sensitivity and specificity of the CMT and MWST were 100%, respectively. The results revealed a strong association between these parameters and the diagnosis of subclinical mastitis in milk samples. In conclusion, the bacteria isolated from SCM play an important role on food poisoning especially S.aurus and E.coli.
      PubDate: Sat, 16 Jul 2022 22:00:00 +010
  • Molecular detection of virulence and resistance genes of Enterococcus
           species isolated from ...

    • Abstract: Abstract Enterococcus spp. as one of the most serious pathogens that could pose risk to animal and human. So, this study aimed to focus alight on virulence of this microbs and to show the releation between antimicrobial resistance and biofilm formation in Enterococcus spp. Atotal of 120 samples of raw milk and cheese were collectedfrom diferent markets in Qena province,Egypt.The conventional identification showed that 30 isolate were suspected to be Enterococcus species, VITEK 2 system confirmed isolates as Enterococcus and classified into15 isolates were Enterococcus fecalis ,10 isolates were Enterococcus faecium whereas the least species were 5 isolates were Enterococcus galliarum.The results of antimicrobial susptability showed that high resistance was recorded against pencillin, Erythromycine, Amoxacillin, Nitrofurton and Cefotaxime. Ten isolates were selected and tested by PCR for detection antimicrobial resistance and biofilm genes.the results showed that our isolates harbored different antimicrobial restance genes as ermB (80%), optrA (0%), blaZ(100%) and vanA (30%) biofilm formation genes hyl and ef3314 (100%), esp (90%).Also, our study is fuelling the concern on significant releationship between biofim formation and specific types of antimicrobial resistance in Enterococcus spp. As Cefatoxime, Pencillin, Ampicillin and Erythromycin. So, the deterrence of the contamination by Enterococcus spp. in milk and its products becomes necessary by applying strict hygenic measure .
      PubDate: Thu, 07 Jul 2022 22:00:00 +010
  • Evaluation the antibacterial effect of hydroalcoholic coffee extract on L.

    • Abstract: A total of 450 samples of raw milk, pasteurized milk, flavored milk, dominate cheese, kareish cheese, small scale ice cream, large scale ice cream, small scale yoghurt and large scale yogurt. Samples were inspected for incidence of Listeria spp. and genetic detection of 16S rRNA gene by PCR. Listeria spp. isolated from 27 (6%) out of total samples examined. Listeria spp. recovered from tested market raw milk, kareish cheese, small scale ice cream and domiati cheese samples with 20, 16, 14% and 4%, respectively. However, Listeria spp. was absent in other analyzed samples. The most prevalent species was L. monocytogenes 10 (2.22%), followed by L. grayii 9 (2%), L. welshimeri 4 (0.89%) and L. innocua 3 (0.67%) with overall 5.7% verified by PCR. Eight out of 10 L. monocytogenes isolates confirmed by 16S rRNA gene that implies risk of food-borne listeriosis among dairy product consumers. Furthermore, a unique approach used for assessment antibacterial activity of different concentrations of gentamicin and hydroalcoholic coffee extract (H. A. coffee extract) against isolated L. monocytogenes strain in vitro using a microplate in ELISA reader is provided in current work. The potent antimicrobial activity of gentamicin and H. A. coffee extract obtained at 30 µg/ml against isolated L. monocytogenes strain. At which not exhibit listeria growth up to 98.3 % and 96.1 %, respectively. H. A. coffee extract was screened using gas chromatography-mass spectrometry (GC-Mass). This analysis showed that antibacterial impact of H. A. coffee extract against L. monocytogenes is attributed to caffein and eugenol.
      PubDate: Thu, 30 Jun 2022 22:00:00 +010
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