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  Subjects -> VETERINARY SCIENCE (Total: 225 journals)
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Veterinarski Glasnik
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  This is an Open Access Journal Open Access journal
ISSN (Print) 0350-2457 - ISSN (Online) 2406-0771
Published by U of Belgrade Homepage  [9 journals]
  • Multiple omental hemangiomas in a Himalayan cat: Incidental finding in a

    • Authors: Amir Amniattalab; Amin Rezazadeh
      Abstract: Himalayan cat. The cat was affected by hemorrhagic gastroenteritis caused by Fusobacterium necrophorum. Also, chronic renal failure (CRF) was demonstrated according to high levels of blood urine nitrogen (BUN), creatinine (Cr), as well as hypoproteinemia and anemia. In this respect, in urinalysis, urine specific gravity (USG) decreased while urea, creatinine and total protein levels increased. Moreover, the complete blood count (CBC) tests showed neutrophilia, monocytosis and lymphopenia. During an exploratory laparotomy, nine masses with a size of 1-5 mm and firm consistency were found to be scattered on the omentum. Histologically, the masses consisted of capillary-cavernous vessels with well-differentiated endothelial cells. No mitotic figures, hemorrhage, or necrosis were found, but there was focal lymphocytic infiltration in the parenchyma of the masses. Immunohistochemically, expression of vimentin and von Willebrand factor (vWF) was found in the endothelial cells, while the immunoreaction to smooth muscle actin (αSMA) was negative. These findings confirmed the diagnosis of hemangioma. To the best of the authors’ knowledge, this is the first report of feline omental hemangioma.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • A Case of a Simple Solitary Renal Cyst in a Shih Tzu

    • Authors: Erdem Gülersoy; Elgin Orçum Uzunlu, Büşra Burcu Erol
      Abstract: In this report, a clinical case of a solitary renal cyst in a Shih Tzu dog is described. On the basis of clinical and laboratory examinations, and ultrasonographic and radiologic visualizations, provisional and differential diagnoses were made. The Shih Tzu dog described in this report was referred to the Small Animal Hospital of the Faculty of Veterinary Medicine, Selçuk University, for further work-up after a one-week treatment in a private veterinary clinic. In our hospital, a simple solitary renal cyst was detected and was treated successfully with ultrasound-guided percutaneous drainage. The dog was followed-up during two months’ post procedure with renal ultrasonography and four months by phone calls with the owner. The initial complaints such as anorexia and lethargy disappeared after the treatment. The ultrasonographic appearance of the cyst changed from an ovoid anechoic structure into a vague, slightly hyperechoic scar lesion.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Mucoid bovine mastitis isolates of Streptococcus uberis –
           difficulties in identification

    • Authors: Oliver Stevanović; Dubravka Milanov, Milan Đilas, Nevenka Aleksić
      Abstract: Highly mucoid Streptococcus uberis were isolated on blood agar from five milk samples originating from two dairy cow farms. All the isolates were CAMP test and esculin hydrolysis negative. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) following prior extraction with 70% formic acid was used for identification to the species level. Given that it is plausible that such strains could be isolated in the future from dairy cows by laboratories in Serbia and the Balkans, we consider this case report to be a useful instruction for veterinary bacteriology laboratories.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Animal models in bicompatibility assessments of implants in soft and hard

    • Authors: Bolka Bogomir Prokić ; Tijana Lužajić Božinovski, Vladimir Gajdov, Ivan Milošević, Vera Todorović, Marija Đošić, Vesna Mišković-Stanković, Danica Markovic
      Abstract: The ethical dilemmas of using animals as in vivo models in preclinical and clinical examinations have been increasingly present in recent decades. Small laboratory animals (rats, rabbits) will continue to be used because they are cost-effective and permit the formation of statistically testable cohort groups; a task that, for financial, maintenance and care reasons, is almost prohibitive for larger animals. Technological advances in the production of new biomaterials for clinical use are enormous, but screening tests and methods used to assess biocompatibility lag behind these advances. The assessment of biological responses is slow and based on millennial recovery mechanisms in eukaryotic organisms. Therefore, the goal of researchers in this field is to re-evaluate old methods of biocompatibility assessment and introduce new methods of evaluation, especially for in vivo testing. In that sense, a revision of the ISO standards was planned and conducted in 2017, which insisted on cytotoxicity testing in cell lines and produced concrete proposals on how biocompatibility should be quantified. In vivo biocompatibility evaluation of biomaterials used for soft tissue recovery commonly utilises rats. Rabbits are recommended for implants used for hard tissues, because of the rabbit’s size, the possibility of implanting the biomaterials on a larger bone surface, and because of the peculiarities of rabbit bone tissue that favours rapid recovery after bone defects and enables easy reading of the results.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Use of allele specific pcr to investigate the presence of β-casein
           polymorphism in holstein-friesian cows

    • Authors: Marko Ristanic; Aleksandar Niksic, Mia Niketic, Stefan Jelisic, Milan Rajkovic, Uros Glavinic, Zoran Stanimirovic
      Abstract: Following the “one health” principle, we have conducted optimization of a protocol for β-casein genotyping in cattle in order to select cows with exclusively the A2A2 genotype. Gastrointestinal proteolysis of A1 β-casein in humans releases beta-casomorphin 7, which is believed to cause a number of diseases/conditions (diabetes mellitus type 1, ischemic heart disease, atherosclerosis, sudden infant death syndrome, autism, schizophrenia, gastrointestinal discomfort, and prolonged gastrointestinal passage time). On the contrary, A2 β-casein does not cause similar effects on human health, due to its different metabolism. DNA extraction was conducted from blood samples belonging to the laboratory archive of the Department of Biology, Faculty of Veterinary Medicine, University of Belgrade. Determination of genotypes was performed using the Allele Specific Polymerase Chain Reaction (AS-PCR) method. The amplification was preceded by determination of proper primer annealing temperature (65.50 °C), in order to ensure optimal genotyping results. The results obtained indicated a higher frequency of the A2 allele (0.56) compared to the A1 allele (0.44). Furthermore, in 7 out of 35 tested samples, the A1A1 genotype (20.00%) was found, in 17 samples, the A1A2 genotype (48.60%) was found, and in 11 samples, the A2A2 genotype (31.40%) was found. The molecular methods used ensured reliable β-casein genotyping that would enable selection of cows with the A2A2 β-casein genotype, implying production of milk free of the undesirable A1 β-casein protein with all its potential negative impacts on human health.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Acid-base, electrolyte and oxidative status in dairy cows at different
           stages of the production cycle

    • Authors: Ljubomir Jovanović; Sreten Nedić, Dušan Bošnjaković, Svetlana Milanović, Milica Stojić, Ivan Vujanac, Radiša Prodanović, Danijela Kirovski
      Abstract: Previous studies in the field of acid-base and oxidative status in cows have mainly focused on the transition period (three weeks before and three weeks after calving). The aim of this study was to determine the differences in the parameters of acid-base and oxidative status and electrolyte balance in cows not only during the transition period, but also at other stages of the production cycle. Holstein-Friesian cows were divided into four numerically equal groups (n = 6): early lactating cows (9±2 days in milk - DIM), peak lactating cows (50±5 DIM), late lactating cows (170±10 DIM) and dry cows (10±1 days before calving). Venous blood samples were taken from the cows to analyze acid-base status, electrolyte concentrations and oxidative stress parameters, and to compare group means. Significantly higher pH was observed in early lactating cows than in late lactating cows. Sodium (Na+) concentration was significantly lower in early lactating and peak lactating cows compared to dry cows, while chloride
      (Cl-) concentration was also lower in late lactating cows compared to dry cows. Plasma glutathione peroxidase (GSH-Px) activity was higher in early lactating cows compared to peak lactating cows. Thus, the highest blood pH, lowest Na+ and Cl- concentrations and highest serum GSH-Px activity were observed in early lactating cows compared to other groups, indicating the highest metabolic and oxidative stress during this period. In conclusion, it would be useful to consider the inclusion of these parameters in standard health assessment procedures in intensive dairy production.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Retained bovine placenta – various treatments and their effects

    • Authors: Milan Maletić; Filip Spasojević, Jovan Blagojević, Nevenka Aleksić, Slobodanka Vakanjac, Jelena Maletić, Janko Mrkun
      Abstract: Given the influence of retentio secundinarum (RS) on the reproductive performance of cows, the current work aimed to assess the effect of various approaches to the treatment of retained placenta on the main reproductive parameters, primarily on the interval from parturition to the first service, and the insemination index. The trial involved 120 dairy cows in which the placenta was not expelled within 24 h postpartum. The first group (G1, n=60) contained cows in which the retained placenta was not extirpated, but they were administered antibiotics. The second group (G2, n=60) comprised cows in which the placenta was removed manually, and were also administered antibiotics. At the end of the study, 53 cows remained in G1 and 54 cows in G2. According to medicament therapy, both groups were divided into three sub-groups treated with: procaine benzylpenicillin, chlortetracycline hydrochloride or ceftiofur. Comparison of the performance of G1 and G2 cows showed significantly better fertility parameters occurred in the animals from which the retained placenta was extracted manually. The pregnancy rate in the G2 group was remarkably higher than in the G1 group. The average service interval was significantly lower (p<0.05) in the G2 group compared to the G1 group. The best results were obtained with the i.m. administration of ceftiofur after the removal of the placenta.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • A serological survey of canine leptospirosis in the city of Belgrade,

    • Authors: Dragica Vojinović; Jadranka Žutić, Ana Vasić, Slobodan Stanojević, Ljiljana Spalević, Zorana Zurovac Sapundžić
      Abstract: Canine leptospirosis is a zoonosis caused by bacteria belonging to the genus Leptospira. Dogs are one of the animal species involved in the cycle of preservation and transmission of leptospirosis in urban areas. Serological testing for the presence of specific antibodies against Leptospira spp. in dogs was continuously performed between 2010 and 2020 in the city of Belgrade. At the request of the owners themselves, other veterinary laboratories or laboratory clinics, 179 blood sera from 179 dogs were examined in the Laboratory for Immunology, Scientific Institute of Veterinary Medicine of Serbia. Blood sera samples from dogs were examined using the standard microscopic agglutination test (MAT) for the presence of specific antibodies against seven different serovars of Leptospira: Pomona, Icterohaemorrhagiae, Grippotyphosa, Sejroe, Canicola, Bataviae, and Australis. The number of seropositive dogs was 17/179 (9.5%). Among all examined sera, the highest titre of seropositive samples was to serovar Icterohaemorrhagiae (10/17, 58.8%), followed by Pomona (4/17, 23.5%), and serovar Canicola (3/17, 17.6%). Specific antibodies for serovars Grippotyphosa, Sejroe, Bataviae and Australis were not detected in any of the dog sera. Cross-reaction (the presence of two or three titres with different values where one of them was higher than others) between different serovars was diagnosed in a low number of sera (n=4), with the following serovars: Icterohaemorrhagiae and Pomona (n=3) and Pomona and Canicola (n=1). The confirmed specific antibody titres for Leptospira spp. were between 1:100 to 1:3000 (5 sera had titres of 1:100, 7 had titres of 1:300, 4 had titres of 1:1000, and 1 serum had a titre 1:3000). Monitoring canine leptospirosis is a useful tool in preventing leptospirosis in Belgrade.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
  • Profile of veterinary drugs in a specialized pharmacy for manual
           production of drugs in João Pessoa, Brazil

    • Authors: Jhennyfer Barros; Iago Dillion Cavalcanti
      Abstract: This is a cross-sectional study conducted in a veterinary pharmacy for manual production of drugs located in the city of João Pessoa, Brazil. The data collected comprised the period from January 2017 to December 2019, using the records system present in the pharmacy itself. We are seeing a growing demand for veterinary manipulated drugs, with a 40% increase in sales from 2017 to 2019. Among the pharmaceutical forms, capsules were the most commonly requested, followed by solutions, and among the active ingredients, itraconazole was the most frequent. With the diversity of pet species, the manipulation pharmacies have arisen to supply market needs, enabling the development of pharmaceutical forms that meet the physical characteristics of each pet species.
      PubDate: Tue, 26 Apr 2022 00:00:00 +020
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