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Jurnal Sain Veteriner
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  This is an Open Access Journal Open Access journal
ISSN (Print) 0126-0421 - ISSN (Online) 2407-3733
Published by Universitas Gadjah Mada Homepage  [48 journals]
  • POLA PEMELIHARAAN BURUNG WALET PADA PULAU-PULAU UTAMA PENGHASIL SARANG
           BURUNG WALET DI INDONESIA

    • Authors: Dede Sri Wahyuni, Hadri Latif, Mirnawati B Sudarwanto, Chaerul Basri
      Pages: 117 - 127
      Abstract: Burung walet di Indonesia umumnya dibudidayakan pada rumah burung walet (RBW) secara tradisional dengan pola pemeliharaan tertentu. Pola pemeliharaan menjadi salah satu faktor pendukung bagi burung walet untuk memproduksi sarang burung walet (SBW) dengan kualitas baik secara berkelanjutan. Penelitian ini bertujuan untuk menganalisis pola pemeliharaan burung walet di RBW di pulau-pulau utama penghasil SBW di Indonesia. Suatu survei terhadap total 44 RBW di pulau Jawa, Sumatera, Sulawesi, dan Kalimantan telah dilakukan untuk mengetahui pola pemeliharaan di masing-masing pulau tersebut. Data dikumpulkan melalui wawancara secara langsung dengan menggunakan kuesioner terstruktur. Pertanyaan dalam kuesioner terdiri atas karakteristik bangunan, kebersihan, sumber makanan dan udara, dan lingkungan RBW. Hasil penelitian menunjukkan bahwa bangunan RBW umumnya bertingkat, dengan atap beton, dinding bata semen, lantai plester semen, dan sirip kayu. Rumah burung walet dibersihkan dibersihkan dengan cara digores/disapu dalam waktu kurang dari dua bulan. Kotoran burung walet umumnya digunakan untuk kebutuhan sendiri. Rumah burung walet umumnya tidak menyediakan bahan untuk menarik serangga atau pakan tambahan. Pakan burung walet umumnyaHymenoptera , dan sumber airnya adalah kolam di dalam gedung RBW. Lingkungan RBW merupakan kawasan pemukiman dan dekat dengan jalan raya. Pembinaan dan pemantauan terhadap pola pemeliharaan burung walet perlu terus dilakukan untuk mendapatkan SBW yang berkualitas baik. 
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.69112
      Issue No: Vol. 40, No. 2 (2022)
       
  • Kajian Daun Sirih Hijau (Piper betle L) Sebagai Antibakteri

    • Authors: Hilma Halimatus Sadiah, Adi Imam Cahyadi, Sarasati Windria
      Pages: 128 - 138
      Abstract: AbstractBacterial infection is one of the biggest health issues in animals and humans. Antibiotic is commonly used in bacterial infection therapy, yet antibiotic misuse can lead into bacterial antibiotic resistance. This means antibiotic is less effective in therapy of resistant bacteria. Green betel leaf has been known as traditional herbal medicine with rich essential oil and consists of phenol with several derivatives that have antibacterial activity. The results of the research that have been carried out can be reported that green betel leaf has the potential to be antibacterial. This herbal can be used as alternative therapy because it is easy to get and have low-level toxicity. In this study we try to discuss about green betel leaf (Piper betle L) as antibacterial with activity against Gram-positive and Gram-negative bacteria by analyzing the component of the active compound and mechanism of action.keywords: antibacterial activity; green betel leaf; Piper betle L 
      AbstrakInfeksi bakteri menjadi masalah yang sudah sering terjadi dan berdampak buruk bagi kesehatan manusia dan hewan. Pengobatan untuk penyakit infeksi biasanya menggunakan antibiotik, namun banyak penggunaan antibiotik yang disalahgunakan sehingga dapat mengakibatkan terjadinya resistensi dan bakteri menjadi tidak sensitif terhadap pengobatan antibiotik. Daun sirih hijau merupakan salah satu obat tradisional yang memiliki kandungan minyak atsiri dan komponen utamanya terdiri fenol dengan beberapa turunannya yang berperan sebagai antibakteri. Hasil dari penelitian yang telah dilakukan dapat dinyatakan bahwa daun sirih hijau dapat berpotensi sebagai antibakteri. Selain itu daun sirih hijau mudah didapat dan memiliki toksisitas rendah yang menyebabkan tanaman tradisional tersebut dijadikan sebagai alternatif dalam mengobati infeksi bakteri. Studi literatur ini membahas daun sirih hijau (Piper betle L) sebagai antibakteri terhadap bakteri Gram positif dan Gram negatif meliputi komponen senyawa aktif dan mekanismenya.Kata kunci : aktivitas antibakteri; daun sirih hijau; Piper betle L
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.58745
      Issue No: Vol. 40, No. 2 (2022)
       
  • Farmers' Characteristics Associations with the Knowledge for Sheep
           Estrus Detection in Batur Village, Banjarnegara, Indonesia

    • Authors: Alek Ibrahim, Wayan Tunas Artama, Rini Widayanti, Bayu Andri Atmoko, I Gede Suparta Budisatria, Dyah Ayu Larasati
      Pages: 139 - 146
      Abstract: The accuracy of the estrus detection was important in sheep breeding management. This study aimed to determine the correlation between the farmers’ characteristics and the knowledge on estrus detection of their sheep. The data were collected by interviewing 33 farmers, who have raised sheep with a breeding system for at least two years of experience in Batur Village, Banjarnegara, Indonesia. The farmers’ knowledge for estrus detection was divided into three-level, namely low, medium, and high levels. The results showed that the farmers’ knowledge for estrus detection was 51.5%, 27.3%, and 21.2% on the low, medium, and high levels, respectively. There was a significant relationship between formal education, informal education, number of sheep, the sheepfold position, stay duration in the sheepfold, and the ram ownership for mating toward knowledge of estrus detection level. It can be concluded that there is a relationship between farmer characteristics in Batur Village and the ability to detect estrus in ewes, which is dominated at low levels, so it is necessary to increase farmers' knowledge regarding estrus detection.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.68462
      Issue No: Vol. 40, No. 2 (2022)
       
  • The Vermicidal Activity of Water Extract Two Types of Ethnopharmacology
           Against Haemonchus contortus in Kacang Goat (Capra hircus) In-vitro

    • Authors: I Gusti Komang Oka Wirawan, Suryawati -, Theresia Nur Indah Koni, Redempta Wea
      Pages: 147 - 154
      Abstract: This research aims at determining the differences in the vermicidal activity between EADMAN and EADMDV in-vitro based on concentration and treatment time. It encompassed three groups of concentrations of EADMAN and EADMDV solution (2.5%, 3.5%, 4.5% from 0.2 g/mL each extract solution), a group of negative control (aqua pro injection), and a group of positive control (Oxfendazole 0.055%). The EADMAN and EADMDV was diluted in 1.5 mL aqua pro injection, then placed on petri dish with a concentration of 2.5%, 3.5%, 4.5% of 0.2 g/mL extract solution. Eight worms placed on each petri dish afterwards. The effectivity of EADMAN and EADMDV was determined based on the percentage of dead worms. The dead and live worms was counted from each immerse group for 1, 3, 5, 7 hours with four replications for each treatment. The vermicidal activity was indicated by touching the worms using a spatula; if it did not react, it was put in warm water. If the worms did not move, it was declared as the dead one, otherwise it was paralysed. Data of effective concentration from both extracts against vermicidal activity was analyzed using a two-way Anova, followed by Tukey test (P>0.05). The result showed the vermicidal activity of 2,5% EADMDV was significantly greater than 4.5% EADMDV, the negative control, and EADMAN at all concentrations. Meanwhile, its difference to 3.5% EADMDV and positive controls revealed insignificant results statistically. Therefore, 2.5% concentration of EADMDV with 7 hours of immersions gave the most effective vermicidal activity against Haemonchus contortus.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.70779
      Issue No: Vol. 40, No. 2 (2022)
       
  • Microbiological Quality of Milk Flavored Ready to Drink and Hygiene
           Practice of Street Vendors

    • Authors: Herwin Pisestyani, Irena Ivania, Ardilasunu Wicaksono, Denny Widaya Lukman, Mirnawati Sudarwanto
      Pages: 155 - 162
      Abstract: Milk products consumption has become a new trend of healthy lifestyle among students in order to meet appropriate daily nutrients. This study was aimed to measure the total number of microbes found in milk drinks sold around the IPB Dramaga and Cilibende and to determine factors that influence it. Samples were collected from 13 milk vendors within radius of 2 kilometers from outermost point of campus and taken with 3 repetitions. Calculation of the total number of microbes was done by using the plate count method in accordance with SNI 2897-2008 about testing method of microbial contamination in meat, eggs, and milk, as well as processed products and SNI 01-6366-2000 about the maximum limit of microbial contamination in food. Risk factors’ data was taken by interviewing milk drinks seller using structured questionnaire. Factors that influenced microbial contamination were analyzed using Chi-Square test. The results showed that 74.4% of milk drinks samples had a high total number of microbes. The average total number of microbes in Dramaga and Cilibende samples was 1.0 x 105 ± 1.4 x 105 cfu / ml and 1.1 x 107 ± 1.2 x 107 cfu/ml. The total number of microbes are significantly influenced (p<0.05) by equipments used, the cleanliness of the equipment, the cleanliness of the table, washing hands practice, and the distance from the crowd. The high number of microbial contamination in milk drinks could damage the quality of milk and shorten the shelf life.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.60996
      Issue No: Vol. 40, No. 2 (2022)
       
  • Uji In Vitro Efektivitas Ekstrak Biji Jintan Hitam (Nigella Sativa L.)
           terhadap Pertumbuhan Microsporum gypseum Penyebab Dermatitis pada Anjing

    • Authors: Pasha Glabella, Salma Ramandhanti Putri, Ery Haryani, Agnesia Endang Tri Hastuti Wahyuni
      Pages: 163 - 170
      Abstract: Biji jintan hitam (Nigella sativa L.) mengandung senyawa aktif seperti thymoquinone, carvacrol, dan thymol yang berperan sebagai antijamur. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Microsporum gypseum yang merupakan salah satu agen penyebab dermatitis pada anjing. Kemampuan ekstrak biji jintan hitam dalam menghambat pertumbuhan jamur Mircrosporum gypseum diuji dengan metode difusi agar dengan teknik sumuran (Agar Well Diffusion). Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak biji jintan hitam terhadap pertumbuhan jamur Microsporum gypseum yaitu 10%, 25%, 50%, 75%, 100%, flukonazol digunakan sebagai kontrol positif dan phospat buffer saline (PBS) kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak biji jintan hitam dengan konsentrasi mulai dari 25%, 50%, 75% dan 100% dapat menghambat pertumbuhan jamur dengan rerata lebar zona hambat secara berurutan ± 3,67 mm., ± 2,84 mm., ± 3,67 mm., dan ± 4,00 mm. Ekstrak biji jintan hitam mampu menghambat pertumbuhan Microsporum gypseum.  Perlu penelitian lebih lanjut untuk mendapatkan hasil optimal sehingga berpeluang sebagai anti fungal pada penyakit dermatitis pada anjing.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.69251
      Issue No: Vol. 40, No. 2 (2022)
       
  • Early Detection of Contamination Vibrio parahaemolyticus and Escherichia
           coli in Fisheries Product Using Multiplex Polymerase Chain Reaction

    • Authors: Nur Hasanah, Putu Eka Sudaryatma, Imanuddin Razaq, Ni Nyoman Eriawati, Wahyu Andy Nugraha, Hidayati Kumalasari, Ni Putu Arya Shintya Anggraeni, Ida Ayu Mirah Meliana Dewi
      Pages: 171 - 182
      Abstract: The fisheries sector provided a significant contribution to the Indonesian economy by increasing export activities in every year. The exported fisheries product are categorized of live fish, frozen fish, preservation products from various types of fish, crustaceans, and molluscs. The contamination of pathogenic bacteria Vibrio parahaemolyticus and Escherichia coli causing healthy problems originating from the fishery sector (sea-food borne disease). These two bacteria contaminated fisheries product is due to mishandling and storaging in the processing, which causes acute diarrhea, gastrointestinal infections and fever. The multiplex polymerase chain reaction (mPCR) method was developed to increase the efficiency of time, effort and accuracy of the bacterial contamination testing process. The mPCR method begins with the optimization of the two bacterial gene targets, sensitivity test, specificity test and then applied to samples of fishery products. The mPCR method is carried out in two mechanisms, namely “one-run” conducted from bacterial colonies isolated on agar media and “one-tube” which is applied directly from fishery products. The results of the development of the mPCR method on V. parahaemolyticus and E. coli resulted in sensitivity at concentrations of DNA 5.6 pg/ml and DNA 5.5 pg/ml, respectively. One-tube mPCR application obtained 7 positive colonies of V. parahaemolyticus and 38 positive colonies of E. coli. Meanwhile, one-tube mPCR which was applied directly from shrimp samples could identify the two bacteria.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.73314
      Issue No: Vol. 40, No. 2 (2022)
       
  • In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against
           Haemonchus contortus in The Sheep

    • Authors: Annisa Putri Cahyani, Budi Purwo Widiarso, Supriyanto -, Wida Wahidah Mubarokah
      Pages: 183 - 187
      Abstract: Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.70399
      Issue No: Vol. 40, No. 2 (2022)
       
  • Infection of Infectious Spleen and Kidney Necrosis Virus and Viral
           Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase
           Chain Reaction

    • Authors: Hidayati Kumalasari, Putu Eka Sudaryatma, Artanti Tri Lestari, Wahyu Nurlita, Wahyu Andy Nugraha, Nur Hasanah, Ida Ayu Mirah Meliana Dewi, Ni Putu Arya Shintya Anggraeni
      Pages: 188 - 196
      Abstract: Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN. 
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.73599
      Issue No: Vol. 40, No. 2 (2022)
       
  • Comparative of Neutrophils and Lymphocyte Ratio for Dairy Cattle in
           Prepartum and Postpartum Condition

    • Authors: Diza Raudya Tuzzahra, Eko Fuji Ariyanto, Septiyani -, Aziiz Mardanarian Rosdianto
      Pages: 197 - 204
      Abstract: There is a transition period that dairy cattle always pass through in their reproduction cycle, making them more stressed. Stress can simply be identified by the ratio of neutrophil (N) and lymphocyte (L) at the haematologic state of dairy cattle. The purpose of this study is to evaluate stress conditions in dairy cattle’s transition period based on neutrophil and lymphocyte ratio. Twenty samples of dairy cattle’s blood in prepartum and postpartum condition are being analyzed with a hematology analyzer. The result of this study shows that the average of neutrophil has increased insignificantly from prepartum to postpartum period as 4.14 103/µL ± 1.51 to 4.27 103/µL ± 2.08 or 1.03% with a normal interval of neutrophil is 1.7-6.0 103/µL. However, the average of lymphocytes decreased insignificantly from 3.23 103/µL ± 1.13 to 3.03 103/µL ± 1.23 or 0.93% with a normal interval of lymphocyte is 1.8-8.1 103/µL. Then, the average of neutrophil and lymphocyte ratio has increased insignificantly from 1.35 103/µL ± 0.46 to 1.47 103/µL ± 0.60 or 1.09%. Based on this result, the ratio of neutrophils per lymphocyte still in the normal range. In the transition period, only a few dairy cattle have a stressful condition. The stress condition in dairy cattle can be identified by increasing neutrophil (neutrophilia) and reducing lymphocyte (lymphocytopenia).
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.67404
      Issue No: Vol. 40, No. 2 (2022)
       
  • Komparasi Lima Jenis Primer Polymerase Chain Reaction Untuk
           Mengidentifikasi Kelamin Burung Famili Columbidae Yang Akurat

    • Authors: Fauziah Fitriana, Riza Resita, Yuda Disastra, Gioknio Happy Alfatik, Clara Ajeng Artdita, Aris Haryanto, Fatkhanuddin Aziz
      Pages: 205 - 220
      Abstract: Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.68787
      Issue No: Vol. 40, No. 2 (2022)
       
  • Penyakit Chronic Respiratory Disease (CRD) : Etiologi, Epidemiologi,
           Patogenesis, Gejala Klinis, Diagnosis, Pengobatan dan Pencegahan

    • Authors: Desy Cahya Widianingrum, Satrio Adi Prakoso, Mila Riskiatul Rohma, Muhammad Faza Hunafah, Muhammad Iqbal, Dhimas Yusantoro
      Pages: 221 - 224
      Abstract: Agen penyebab penyakit Chronic Respiratory Disease (CRD) berasal dari bakteri Mycoplasma gallisepticum yang menginfeksi saluran pernapasan pada ternak unggas seperti ayam, itik, angsa, entok, kalkun, burung dara, dan lain-lain. Faktor yang dapat memperburuk terjadinya infeksi penyakit ini yakni umur ternak, jenis kelamin, stres, dan lingkungan. Infeksi bakteri ini lebih rentan pada ayam yang berumur muda dan ayam betina dibandingkan dengan ayam dewasa dan ayam jantan. Gejala klinis dari penyakit ini yaitu terdengarnya suara ngorok pada ayam di malam hari, keluarnya catarrhal dari rongga hidung, batuk, radang conjunctiva, dan bersin. Masa penyakit CRD berkisar antara 4 - 21 hari dan mudah menular. Metode uji laboratrium untuk mengidentifikasi pemeriksaan terhadap kontaminasi bakteri Mycoplasma gallispeticum di kandang diantarnya uji seroligi seperti HI (Hemaglutination Inhibition Test), RSA (Rapid Serum Aglutination Test), maupun ELISA (Enzyme Linked Immuno Sorbent Assay). Kejadian penyakit CRD hingga saat ini yang masih ditemukan di seluruh wilayah Indonesia menyebabkan kerugian bagi peternak. Kerugian ekonomis akibat penyakit ini dapat diminimalisir dengan pengetahuan peternak akan pentingnya program biosekuriti yang harus diterapkan secara konsisten, serta peningkatan imunitas ternak seperti vaksinasi serta penggunaan antimikroba yang aman dan tidak menimbulkan residu. 
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.56683
      Issue No: Vol. 40, No. 2 (2022)
       
  • Combination of Acupuncture Points in The Treatment of Hind Leg Paresis
           with Electroacupuncture Methods in Domestic Cats

    • Authors: Mudhita Zikkrullah Ritonga, Tongku Nizwan Siregar, M Hanafiah, Muttaqien -, Elvina Andali Putri
      Pages: 225 - 231
      Abstract: Back leg paresis is muscle weakness that can cause the inability to walk or support the back of the body. A domestic cat, female, black tabby hair colour with a bodyweight of 2.9 kg has weakness in both hind legs so it cannot lift the back of the body properly but there is still a reflex when pressing using tweezers sirurgis on the gluteus muscle tendon. The flexor reflexes on the digits are still present but slightly. From the physical examination, it was concluded that there was a disturbance in the segment IV spinal cord, precisely at L7-S1. Cats also experience problems with defecation, namely the patient's stool is watery inconsistency. The results of the X-Ray examination showed that the segments of the os. lumbar, os. femur, os. tibia, os. fibula, os. metatarsal to digit there are no fractures and also the visceral organs do not experience changes and abnormalities. However, there is a bias picture, namely compression on the 7th segment (S4-L5, L6 and L7). The diagnosis was that the cat had disorders of the spinal cord segments IV (L4-S2) and V, to be precise, from L7 to S2. Electroacupuncture therapy at points BL-18, BL-19, SP-6, ST-36 and LIV-3 was carried out for three weeks, namely twice in the first week and once in the second and third weeks. Oral drug therapy in the form of Neurobion® tablets is taken orally during therapy.
      PubDate: 2022-08-31
      DOI: 10.22146/jsv.67650
      Issue No: Vol. 40, No. 2 (2022)
       
 
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