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- Optimization of Sybr Green Real Time Polymerase Chain Reaction using
Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma
gondii
Authors: Fitrine Ekawasti, Umi Cahyaningsih, Ni Luh Putu Indi Dharmayanti, Agus Winarsongko, siti sadiah, harimurti nuradji
Pages: 1 - 13
Abstract: AbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosis
PubDate: 2024-04-01
DOI: 10.22146/jsv.90867
Issue No: Vol. 42, No. 1 (2024)
- Performa Estrus dan Hormon Estrogen Sapi Friesian Holstein Postpartus yang
Diberi UMMB Dengan Perekat Tepung Tapioka
Authors: Hasbi Hasbi, Zyahrul Ramadan, Renny Fatmyah Utamy, Ambo Ako, Masturi Masturi, Sri Gustina, Tasya Tasya, Rara Mufliha, Andi Fitri Nurbina, Andi Mutfaidah, Rio Saputra, I Dewa Ayu Mahayani, Mutmainna Mutmainna, Rahman Andi Arif, Sukri Siti Annisa
Pages: 14 - 23
Abstract: The reproductive ability of Holstein Friesian (FH) cows greatly affects milk yield because, however, in the lactation period, dairy cows anestrus postpartus, which is the condition where does not appear in estrus postpartus. Anestrus postpartus to uterine involution as a consequence of nutritional deficiencies. Recovery from can be this condition can be conducted by additional feeding. One of the supplementary feeds is urea multinutrient molasses block (UMMB). UMMB consists of fillers and adhesives and cement is the most used in UMMB production as an adhesive. UMMB is an additional feed using cement dairy cows health problems. Therefore, the objective of this research was to determine the effect of cement substitution with tapioca meal as an adhesive on postpartus estrus of FH cows. A total of 9 lactating FH dairy cows were divided into 3 dietary groups. Each treatment had 3 cows as replicates. The dietary treatments consisted of UMMB with 100% cement adhesive (as D0); 50% cement and 50% tapioca (D1); and 0% cement and 100% tapioca meal (D2). The parameters were postpartus estrus, estrus speed, estrus duration, estrus intensity, and estrogen hormone levels. The results revealed that the treatment had a significant effect (P<0.05) on the duration of estrus, while it had no significant effect (P>0.05) neither on postpartus estrus, estrus speed, estrus intensity, or estrogen hormone levels. Therefore, it can be concluded that tapioca meals can be used as a substitute for cement as much as 50% provides as an adhesive in UMMB production for better estrus performance.
PubDate: 2024-04-01
DOI: 10.22146/jsv.87348
Issue No: Vol. 42, No. 1 (2024)
- Profil Hematologi, Besi, dan Rasio Mieloid:Eritroid Kuda Hiperimun yang
Digunakan dalam Produksi Plasma Antisera
Authors: Dinar Arifianto
Pages: 24 - 31
Abstract: Kuda merupakan salah satu hewan yang penting dalam produksi plasma antisera sebagai bahan baku pembuatan serum. Akhir abad ke-19 kuda berperan krusial dalam pengembangan serum pertama terhadap penyakit difteri manusia. Hingga saat ini kuda masih digunakan untuk memproduksi berbagai serum anti-venom, anti-rabies, anti-tetanus, hingga anti-SARS CoV-2. Proses produksi plasma antisera diawali dengan injeksi imunogen pada kuda yang disebut dengan hiperimunisasi. Antibodi dalam jumlah besar yang terbentuk dikoleksi kemudian dipurifikasi untuk digunakan pada manusia. Hiperimunisasi menimbulkan berbagai efek samping, diantaranya adalah penimbunan fibril amiloid pada berbagai jaringan yang dapat mengganggu fisiologi dan fungsi organ tubuh. Sebanyak 12 ekor kuda digunakan dalam penelitian ini dan dibagi menjadi 4 kelompok. Kuda kontrol (n=3) terdiri atas kuda yang belum pernah mendapatkan perlakuan hiperimunisasi, sedangkan 9 kuda lainnya dikelompokkan berdasarkan lamanya waktu produksi plasma antisera dengan metode hiperimunisasi (masing-masing kelompok berjumlah 3 ekor). Kelompok I terdiri atas kuda dengan masa produksi 2-3 tahun, kelompok II terdiri atas kuda dengan masa produksi 4-5 tahun, dan kelompok III terdiri atas kuda dengan masa produksi 6-7 tahun. Sampel darah, serum, dan aspirasi sumsum tulang dianalisis utuk melihat profil eritrosit, Hb, PCV, trombosit, profil besi (besi serum, UIBC, TIBC, saturasi transferin), rasio mieloid:eritroid (M:E) dan persentase retikulosit. Analisis statistik menunjukkan bahwa terdapat perbedaan yang signifikan pada parameter eritrosit dan rasio M:E (P<0,05), sedangkan Hb, trombosit, dan profil besi tidak ditemukan perbedaan yang signifikan (P>0,05). Hal tersebut menunjukkan bahwa perlakuan hiperimunisasi menyebabkan terjadinya anemia non-regeneratif dengan mekanisme supresi eritropoiesis sumsum tulang tanpa memengaruhi komponen besi.
PubDate: 2024-04-30
DOI: 10.22146/jsv.90681
Issue No: Vol. 42, No. 1 (2024)
- Development of In-House ELISA using recombinant LipL32 for Detection of
Human Leptospirosis in Indonesia
Authors: Sumarningsih Sumarningsih
Pages: 32 - 36
Abstract: Early laboratory confirmation is important for the accurate diagnosis and treatment of patient infected by leptospirosis. However, Microscopic agglutination test (MAT) as the gold standard for detection of human leptospirosis has many limitation and only available in reference laboratories. Therefore, many studies suggested LipL32 protein as a good candidate for development of leptospirosis detection kit because it is highly conserved and produced only in pathogenic Leptospira species. In this study, we aim to investigate the performance of our in-house ELISA using recombinant LipL32 to detect leptospirosis in Indonesia. Fourteen human sera were used in this study and the infection status were determine using MAT. The result showed that nine of eleven MAT positive sera were successfully recognized by LipL32 ELISA. The antibody binding to LipL32 was also confirm by immunoblot. There was one of three MAT negative sera has high OD above 0.5 in ELISA, but it showed negative reaction in immunoblot result. Overall, this study demonstrated that recombinant LipL32 protein can recognized antibody from human leptospirosis and can be used as a universal antigen to detect infection by any serovars of pathogenic leptospira.
PubDate: 2024-04-01
DOI: 10.22146/jsv.90085
Issue No: Vol. 42, No. 1 (2024)
- Deteksi Gen Faktor Virulensi Escherichia Coli Patogen Resistan
Siprofloksasin Asal Usap Kloaka Ayam Layer
Authors: Joen Firmanta Peranginangin
Pages: 37 - 45
Abstract: Avian Pathogenic Escherichia coli (APEC) is a cause of extraintestinal infections, particularly respiratory infections, pericarditis, and septicemia in poultry (colibacillosis). Research aimed at uncovering the virulence factors responsible for colibacillosis in poultry, especially laying hens in Indonesia, is still quite rare. The purpose of this study is to identify the virulence factors responsible for colibacillosis in E. coli strains resistant to ciprofloxacin. The virulence factors targeted in this research are the genes iss, iutA, iroN, ompT, and hlyF. The study utilized 327 E. coli isolates from the archives of the National Veterinary Drug Assay Laboratory, which were collected in 2021 in seven provinces.All isolates were tested for pathogenicity using Congo Red. Subsequently, E. coli strains determined to be pathogenic were tested for their sensitivity to ciprofloxacin using the agar dilution method. Isolates that were both resistant and pathogenic were then examined for the presence of the virulence genes using polymerase chain reaction (PCR). Based on the results of the Congo Red test, it was found that 59 isolates (18%) were pathogenic E. coli. Among the 54 pathogenic E. coli isolates subjected to sensitivity testing, 30 isolates were found to be resistant to ciprofloxacin. Subsequently, PCR analysis of these 30 ciprofloxacin-resistant pathogenic E. coli isolates revealed the presence of the iss gene in 29 isolates (96%), iutA in 23 isolates (76.6%), ompT in 19 isolates (63.3%), hlyF in 14 isolates (46.6%), and iroN in 11 isolates (36.6%). Eight isolates showed the presence of all five virulence genes, while the rest had one to three virulence genes. This study demonstrates that virulence genes are commonly found in ciprofloxacin-resistant pathogenic E. coli. This suggests a potential threat to the effectiveness of ciprofloxacin in managing colibacillosis in laying hens.
PubDate: 2024-04-01
DOI: 10.22146/jsv.90358
Issue No: Vol. 42, No. 1 (2024)
- Kualitas Spermatozoa Sapi Bali Polled yang Diberi Pakan Suplemen Taoge
(Phaseolus radiatus L.)
Authors: Sri Gustina, Hasbi Hasbi, Herry Sonjaya, Sudirman Baco, Husnul Qhatimah, Wandi Saputra, Mutmainna Mutmainna, Ekayanti Mulyawati Kaiin, Tulus Maulana
Pages: 46 - 58
Abstract: The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.
PubDate: 2024-04-30
DOI: 10.22146/jsv.86154
Issue No: Vol. 42, No. 1 (2024)
- Identification of Bovine Rotavirus Group A in Bogor, West Java
Authors: Dyah Ayu Hewajuli
Pages: 59 - 66
Abstract: Abstrak Diare merupakan penyakit yang menyebabkan angka kesakitan yang tinggi pada pedet dan kematian neonatal. Penyakit ini dapat disebabkan oleh beberapa agen penyakit yang berbeda. Rotavirus Grup A (RVA) atau Bovine Rotavirus merupakan salah satu agen infeksi penyebab diare pada pedet. Selanjutnya, diare neonatal pada pedet dapat berdampak pada kerugian ekonomi bagi ternak sapi perah dan sapi potong di seluruh dunia karena menyebabkan gangguan pertumbuhan, meningkatnya biaya perawatan, dan/atau kematian pada hewan sakit. Prevalensi Bovine Rotavirus dapat berbeda antar negara di seluruh dunia. Sirkulasi Bovine Rotavirus pada sapi telah dilaporkan di beberapa negara tetapi sirkulasi Bovine Rotavirus pada sapi di Indonesia belum diketahui. Untuk mengetahui prevalensi Rotavirus grup A atau Bovine Rotavirus (BRV), 100 sampel feses dikoleksi dari pedet dengan gejala klinis diare atau tidak diare di Kabupaten Bogor, Jawa Barat pada tahun 2021. Sampel dianalisis terhadap urutan yang mengkode protein kapsid bagian dalam VP6 (subkelompok) menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Lima dari 100 sampel feses sapi (5%) terdeteksi positif BRV. Pada penelitian ini menunjukkan bahwa kelompok Rotavirus atau Bovine Rotavirus (BRV) telah bersirkulasi di antara ternak sapi di Indonesia, khususnya Kabupaten Bogor. Sampel positif Rotavirus grup A atau Bovine Rotavirus (BRV) dapat diidentifikasi dengan metode diagnosis dini (RT-PCR). Kata kunci : Bovine Rotavirus; RT PCR; Bogor Abstract Diarrhea is the most disease that cause high morbidity in calves and neonatal mortality. This disease can be caused by several different infectious agents. Group A rotaviruses (RVA) or Bovine Rotavirus are one of the infectious agents causing diarrhea in calves. Then, Neonatal calf diarrhea can impact to economic losses to dairy and beef cattle herds worldwide, in consequence of growth disorders, value of treatment, and/or death of sick animals. The prevalence of Bovine Rotavirus can become different in the worldwide. The circulation of these bovine rotavirus in calves from the some region has already been demonstrated but the circulation of bovine rotavirus in Indonesia is not known. To investigate the prevalence of A group Rotaviruses or Bovine Rotavirus (BRV), 100 fecal samples were collected from calves with diarrhea or no diarrhea in Bogor district, West Java at 2021. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) using RT-PCR. Five of 100 specimens of bovine fecal (5%) were detected positive as BRV positive. In this study, A group Rotaviruses or Bovine Rotavirus (BRV) have been circulated among cattle herds in Indonesia, particularly Bogor District. The positive samples of A group Rotaviruses or Bovine Rotavirus (BRV) can be identified using the early diagnosis method (RT-PCR). Keywords : Bovine Rotavirus; RT PCR; Bogor District
PubDate: 2024-04-01
DOI: 10.22146/jsv.90683
Issue No: Vol. 42, No. 1 (2024)
- Pengaruh Penggunaan Grid Terhadap Kualitas Hasil Rontgen Anjing Dan Kucing
Pasien RSH Prof. Soeparwi FKH UGM
Authors: Guntari Titik Mulyani, Agung Budi Pramono, Gadis Martathama
Pages: 67 - 71
Abstract: Dokter hewan praktisi sering menjumpai permasalahan dalam penegakan diagnosis, salah satunya adalah pemanfaatan rontgen atau hasil X-ray yang belum optimal. Kualitas radiografi itu sendiri meliputi densitas, kontras, ketajaman dan distorsi, sedangkan salah satu penyebab yang dapat menurunkan kualitas radiografi adalah radiasi hambur. Penelitian ini bertujuan untuk meningkatkan kualitas radiograf dengan mengurangi radiasi hambur sinar-x menggunakan grid. Penelitian ini menggunakan 10 ekor anjing dan 10 ekor kucing dengan berat badan bervariasi dan fokus rontgen yang berbeda. Setiap hewan dilakukan pengambilan sampel xray sebanyak 2 kali, yaitu foto rontgen menggunakan grid dan tanpa grid pada posisi lateral dan anterior-posterior. Hasil rontgen dibandingkan antara foto rontgen dengan grid dan tanpa grid. Dari penelitian ini diperoleh hasil bahwa pengujian foto rontgen menggunakan grid mampu mengurangi radiasi hambur sehingga lebih mengoptimalkan radiograf dan membantu dokter hewan dalam penegakan diagnosa.
PubDate: 2024-04-30
DOI: 10.22146/jsv.81941
Issue No: Vol. 42, No. 1 (2024)
- PEMBERIAN EKSTRAK DAUN SINGKONG PADA BURUNG PUYUH YANG TERPAPAR SUHU
LINGKUNGAN TINGGI
Authors: koekoeh santoso
Pages: 72 - 81
Abstract: Fenomena iklim tropis, ekuinoks, dan pemanasan global di Indonesia dapat menyebabkan masalah cekaman panas pada peternakan unggas termasuk puyuh. Dampaknya adalah penurunan performa dan produksi puyuh, oleh karena itu perlu dilakukan upaya untuk mengatasi masalah heat stress. Tujuan dari penelitian ini adalah untuk membuktikan potensi ekstrak daun singkong untuk mengatasi cekaman panas pada puyuh dewasa anas. Penelitian dilakukan dengan menggunakan 16 ekor puyuh yang dibagi menjadi 4 kelompok, terdiri dari 4 ekor puyuh sebagai kontrol yang diberi perlakuan suhu 35 °C, dan masing-masing 4 ekor puyuh lainnya diberi perlakuan suhu 35 °C dan diberi ekstrak daun singkong dengan dosis yang berbeda-beda sebesar 5,292 mg/168g, 10,584 mg/168 g, dan 21,168 mg/168 g berat badan. Hasil penelitian menunjukkan bahwa ekstrak daun singkong yang mengandung flavonoid dan klorofil terbukti dapat menurunkan kadar malondialdehida (MDA) pada puyuh yang mengalami cekaman panas. Kualitas telur juga meningkat berdasarkan parameter tinggi albumin, tinggi kuning telur, dan tebal cangkang, namun kadarnya tidak signifikan. Parameter total leukosit, rasio heterofil per limfosit, dan diferensial leukosit tidak menunjukkan perbedaan yang signifikan. Ekstrak daun singkong juga belum terbukti mampu menurunkan total protein pada puyuh yang mengalami cekaman panas. Hasil vaksinasi ND inaktif yang dilakukan satu kali menunjukkan titer antibodi yang rendah pada semua kelompok.
PubDate: 2024-04-30
DOI: 10.22146/jsv.73585
Issue No: Vol. 42, No. 1 (2024)
- Antibacterial Activity Honey Against Staphylococcus aureus and Escherichia
Coli O157:
Authors: Widodo Suwito
Pages: 82 - 89
Abstract: Honey is a natural substance from flower nectar and produced by honey bees. The honey has been used as healing. The study was conducted to determine the antibacterial activity of Blora and Kaliandra honey against pathogenic bacteria such as Staphylococcus aureus (S. aureus) and Escherichia coli O157:H7 (E. coli O157:H7). As a comparison, was used commercial honey. The results showed Blora and Kaliandra honey had antibacterial activity, however the commercial honey has not antibacterial activity. Kaliandra honey has antibacterial activity against S. aureus and E. coli O157:H7 better than Blora and commercial honey. Concentration 25% of Kaliandra honey was able to inhibit S. aureus, while blora honey showed antibacterial activity at 50% and commercial honey did not show antibacterial activity until concentration 50%. Inhibition zone of S. aureus produced by Kaliandra honey with concentration 25 and 50% were 8.0 ± 0.1 mm and 9.0 ± 0.2 mm respectively and concentration 50% Blora honey was 7.3 ± 0.1 mm. Inhibition zone of E. coli O157:H7 produced by concentration 50% Kaliandra honey was 8.0 ± 0.3 mm and Blora honey was 7.7 ± 0.1 mm. The study showed that Blora and Kaliandra honey have antibacterial activity and can be used as alternative substitution in antibiotic therapy.
PubDate: 2024-04-01
DOI: 10.22146/jsv.90498
Issue No: Vol. 42, No. 1 (2024)
- Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri
Staphylococcus aureus asal Isolat Berbeda
Authors: Fatkhanuddin Aziz, Fauziah Fitriana, Dian Ritma Setyorini, Nur Ika Prihanani, Shafira Amalia Putri, Tifa Restyka Maulina, Vira Kartika Dewi, Morsid Andityas, Fajar Budi Lestari, Nurulia Hidayah, Risa Ummami, Achmad Fauzi
Pages: 90 - 98
Abstract: Staphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi.
PubDate: 2024-04-30
DOI: 10.22146/jsv.89815
Issue No: Vol. 42, No. 1 (2024)
- Prosedur Pemotongan dan Kualitas Daging Sapi yang Dipotong di TPH di Kota
Jayapura
Authors: Priyo Sambodo, Fitria Sayuri, Sientje Daisy Rumetor
Pages: 99 - 106
Abstract: Penelitian ini bertujuan untuk mengetahui prosedur pemotongan, kualitas fisik dan cemaran mikroba daging sapi yang dipotong di TPH di Kota Jayapura. Penelitian ini dilakukan di lima TPH yang ada di Kota Jayapura. Sampel daging diambil masing-masing sebanyak 25 gram/ekor/TPH. Pengambilan sampel dilakukan seminggu sekali pada setiap TPH dan diulang 3 kali. Prosedur pemotongan diamati secara langsung. Uji kualitas fisik daging sapi, meliputi: aroma, warna, konsistensi dan pH. Sedangkan uji cemaran mikroba daging menggunakan metode TPC untuk mengetahui jumlah bakteri/kuman dan metode MPN untuk mengetahui jumlah Coliform dan E. coli. Data prosedur pemotongan ditabulasi kemudian dilakukan analisa deskriptif. Kualitas fisik daging dianalisa deskriptif berdasarkan SNI 3932:2008 dan tingkat cemaran mikroba pada daging sapi berdasarkan SNI 7388:2009. Hasil: pada semua TPH pemeriksaan antemortem dan pemeriksaan postmortem tidak dilakukan; berdasarkan warna daging, terjadi penurunan tingkat mutu daging dari mutu II menjadi mutu III; sebanyak 3 sampel (18,75%) melebihi standar normal nilai TPC dan sebanyak 15 sampel (93,75%) memiliki nilai cemaran bakteri Coliform dan E. coli di atas batas normal; Kesimpulan: prosedur pemotongan belum memenuhi kriteria yang telah ditetapkan; kualitas fisik daging menurun berdasarkan parameter warna; dan hampir seluruh sampel daging memiliki cemaran mikroba di atas standar
PubDate: 2024-04-01
DOI: 10.22146/jsv.76115
Issue No: Vol. 42, No. 1 (2024)
- Dampak Terapi Antibiotika Mastitis Periode Kering pada Sapi Perah
Authors: Yanuartono - Yanuartono, Soedarmanto - Indarjulianto, Alsi Dara Paryuni
Pages: 107 - 120
Abstract: AbstrakPeriode kering pada sapi perah didefinisikan sebagai masa istirahat non laktasi sebelum melahirkan dengan tujuan utama meningkatkan produksi susu pada periode laktasi berikutnya. Saat ini secara umum antibiotika juga digunakan untuk terapi maupun pencegahan mastitis saat periode kering. Mastitis adalah salah satu penyakit menular yang paling penting pada sapi perah di seluruh dunia, bertanggung jawab atas kerugian ekonomi yang besar dan dampak negatif pada kesejahteraan sapi maupun manusia disebabkan oleh penurunan produksi susu. Salah satu metode yang digunakan untuk meningkatkan produksi susu pada laktasi berikutnya adalah periode kering yang berkisar antara 40-60 hari sebelum melahirkan. Guna menghindari kejadian mastitis saat periode kering maka saat ini banyak digunakan antibiotika saat memasuki periode kering atau akhir periode kering. Saat ini, sebagian besar dari antibiotika yang digunakan dalam industri susu diterapkan untuk mengendalikan mastitis pada sapi perah periode kering. Banyak Negara menerapkan system pemberian antibiotika pada semua kwartir saat periode kering. Namun demikian penggunaan antibiotika tersebut meningkatkan kekhawatiran munculnya resistensi antibiotika. Tulisan ini akan mencoba mengulas secara sederhana dampak penggunaan antibiotika saat periode kering pada sapi perah.Kata kunci : mastitis; periode kering; antibiotika; resistensi
PubDate: 2024-04-01
DOI: 10.22146/jsv.84752
Issue No: Vol. 42, No. 1 (2024)
- Diagnosis of Feline Panleukopenia Based on Clinical Signs and Polymerase
Chain Reaction in Various Ages of Cats
Authors: Soedarmanto Indarjulianto, Mungky Ema Ramadhani, Yanuartono Yanuartono, Slamet Raharjo, Hary Purnamaningsih, Sitarina Widyarini, Yunita Apriana Milla
Pages: 121 - 128
Abstract: Feline panleukopenia (FPL) is a viral infectious disease caused by the feline panleukopenia virus (FPV) that affects cats of all ages. Clinical symptoms that appear in each individual cat vary greatly, depending on age, immune status, and the presence or absence of secondary infection. The aim of this research was to diagnose the FPL based on clinical signs and polymerase chain reaction (PCR) in cat with various ages. This study used 15 cats that showed one of clinical symptoms including lethargy, anorexia, fever, diarrhea, and vomiting. All cats were examined physically and by PCR of blood, then analyzed descriptively. The results showed that 10/15 (66.7%) cats were <7 months, 4/15 (26.7%) were 7-12 months, and 1/15 (6.6%) was >1 year old. Identification by PCR showed that 100% of the samples positive, so that all of cats diagnosed FPL. Clinical signs that commonly appeared in this study included anorexia (80%), fever (80%), vomiting (73.3%), lethargy (66.7%), and diarrhea (40%). Young cats <7 months commonly showed anorexia, fever, vomiting, and lethargy, cats aged 7-12 months commonly showed anorexia, fever, vomiting, and diarrhea, cat aged >12 months experienced anorexia and vomiting. Concluded that the predominant clinical symptoms of FPL in young cats were anorexia, fever, vomiting and lethargy, whereas in adult cats anorexia, fever, vomiting, and diarrhea. Clinical symptoms can be used for initial screening of FPL, but the causative diagnosis needs to be determined by polymerase chain reaction.
PubDate: 2024-04-30
DOI: 10.22146/jsv.79590
Issue No: Vol. 42, No. 1 (2024)
- Cytotoxic Activity Red Eye Snail (Cerithidea obtusa) Extract on Colon
Cancer Cell WiDr
Authors: Wisnu Jaka Dewa
Pages: 129 - 135
Abstract: Based on data released by Globocan in 2020, the incidence of colorectal cancer is the fourth highest in Indonesia (8.6%) and the third in the world (10%). This disease is hard to treat because the available therapy is less effective. Therefore, it is necessary to develop effective alternative therapies, especially those originating from Indonesia's natural resources, easy to obtain and reproduce. This study aims to determine the potential of red eye snail extract as an anticancer through cytotoxicity tests with the MTT Assay method on colon cancer cells WiDr and DNA fragmentation tests with Hoescth staining. In this study, we used various concentrations of the red-eye snail extract to test with 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) and doxorubicin reagents as positive controls. Absorbance values were read using a microplate reader at a wavelength of 595 nm. The cell absorbance data was converted into cell viability and probit analyzed to obtain the IC50 value. The results showed that the higher the concentration of the extract caused a decrease in the number of WiDr cells and an increase in damage to the structure of WiDr cells. Based on the results of probit analysis, it was found that the IC50 value of the extract was 36.28 µg/mL or classified as moderate cytotoxicity. The DNA fragmentation test showed that at concentrations of 125 ppm and 62.5 ppm, it was able to provide an effect similar to doxorubicin, namely triggering apoptosis in WiDr colon cancer cells.
PubDate: 2024-04-01
DOI: 10.22146/jsv.83921
Issue No: Vol. 42, No. 1 (2024)
- Efektivitas Albendazole terhadap Nematodiasis pada Sapi Perah di
Peternakan Sapi Perah Rakyat Kecamatan Tegalombo Kabupaten Pacitan
Authors: Soedarmanto Indarjulianto, Alek Arisona, Joko Prastowo, Dwi Priyowidodo, Yanuartono Yanuartono
Pages: 136 - 144
Abstract: Nematodiasis adalah penyakit yang disebabkan cacing nematoda yang dapat diderita sapi perah segala usia. Penyakit ini mempunyai gejala klinis antara lain kurus, rambut kusam berdiri dan diare. Albendazole merupakan obat cacing berspektrum luas yang sering digunakan oleh peternak karena mudah didapat. Tujuan penelitian ini adalah menentukan prevalensi, gejala klinis dan efektifitas albendazole terhadap nematodiasis pada sapi perah di peternakan sapi perah rakyat Kecamatan Tegalombo, Kabupaten Pacitan. Penelitian ini menggunakan 50 ekor sapi perah yang tidak bunting. Semua sapi diperiksa secara fisik meliputi kondisi umum sapi dan feses. Sampel feses diambil dan diperiksa adanya telur cacing dengan metode nativ, apung dan Mc Master. Sapi penderita nematodiasis selanjutnya diterapi albendazole dengan dosis 10 mg/kg BB satu kali pemberian secara per oral. Perkembangan hasil terapi diamati dengan cara dilakukan pemeriksaan fisik, pemeriksaan sampel feses pada hari ke 3, 7 dan 14. Hasil penelitian menunjukkan bahwa prevalensi nematodiasis sapi perah sebesar 30%. Sapi penderita nematodiasis menunjukkan feses lembek, adanya telur nematoda pada fesesnya. Telur cacing yang ditemukan adalah kelompok telur Strongyle, Toxocara sp, dan Trichuris sp. Efektivitas albendazole terhadap nematodiasis pada sapi perah masih tinggi yakni >97,87%. Disimpulkan bahwa albendazole mempunyai efektifitas yang tinggi untuk mengobati nematodiasis.
PubDate: 2024-04-30
DOI: 10.22146/jsv.79447
Issue No: Vol. 42, No. 1 (2024)
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