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  Subjects -> VETERINARY SCIENCE (Total: 260 journals)
Showing 201 - 63 of 63 Journals sorted alphabetically
Theoretical and Applied Veterinary Medicine     Open Access   (Followers: 2)
Theriogenology     Hybrid Journal   (Followers: 2)
Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere     Hybrid Journal  
Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere     Hybrid Journal  
Topics in Companion Animal Medicine     Hybrid Journal   (Followers: 4)
Transboundary and Emerging Diseases     Hybrid Journal   (Followers: 4)
Trends in Parasitology     Full-text available via subscription   (Followers: 12)
Tropical Animal Health and Production     Open Access   (Followers: 1)
Tropical Animal Science Journal     Open Access   (Followers: 3)
Tropical Veterinarian     Full-text available via subscription   (Followers: 2)
Turkish Journal of Veterinary and Animal Sciences     Open Access  
Turkish Journal of Veterinary Research     Open Access   (Followers: 3)
Ukrainian Journal of Veterinary and Agricultural Sciences     Open Access   (Followers: 1)
Uludag University Journal of the Faculty of Veterinary Medicine     Open Access  
Van Veterinary Journal     Open Access  
VCOT Open     Open Access  
veterinär spiegel     Hybrid Journal   (Followers: 1)
Veterinaria     Open Access   (Followers: 2)
Veterinaria (Montevideo)     Open Access  
Veterinária e Zootecnia     Open Access  
Veterinaria México     Open Access  
Veterinaria México OA     Open Access   (Followers: 1)
Veterinarski Glasnik     Open Access  
Veterinary Anaesthesia and Analgesia     Hybrid Journal   (Followers: 17)
Veterinary and Comparative Oncology     Hybrid Journal   (Followers: 14)
Veterinary and Comparative Orthopaedics and Traumatology     Hybrid Journal  
Veterinary Clinical Pathology     Hybrid Journal   (Followers: 8)
Veterinary Clinics of North America: Equine Practice     Hybrid Journal   (Followers: 13)
Veterinary Clinics of North America: Exotic Animal Practice     Full-text available via subscription   (Followers: 6)
Veterinary Clinics of North America: Food Animal Practice     Full-text available via subscription   (Followers: 6)
Veterinary Clinics of North America: Small Animal Practice     Full-text available via subscription   (Followers: 25)
Veterinary Dermatology     Hybrid Journal   (Followers: 8)
Veterinary Immunology and Immunopathology     Hybrid Journal   (Followers: 15)
Veterinary Journal     Hybrid Journal   (Followers: 19)
Veterinary Journal of Mehmet Akif Ersoy University / Mehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisi     Open Access  
Veterinary Medicine and Animal Sciences     Open Access   (Followers: 4)
Veterinary Medicine and Science     Open Access   (Followers: 2)
Veterinary Medicine International     Open Access   (Followers: 6)
Veterinary Medicine: Research and Reports     Open Access   (Followers: 2)
Veterinary Microbiology     Hybrid Journal   (Followers: 9)
Veterinary Nurse     Full-text available via subscription   (Followers: 5)
Veterinary Nursing Journal     Hybrid Journal   (Followers: 3)
Veterinary Ophthalmology     Hybrid Journal   (Followers: 9)
Veterinary Parasitology     Hybrid Journal   (Followers: 7)
Veterinary Parasitology : Regional Studies and Reports     Full-text available via subscription  
Veterinary Parasitology : X     Open Access  
Veterinary Pathology     Hybrid Journal   (Followers: 21)
Veterinary Quarterly     Open Access   (Followers: 1)
Veterinary Radiology & Ultrasound     Hybrid Journal   (Followers: 13)
Veterinary Record     Hybrid Journal   (Followers: 25)
Veterinary Record Case Reports     Hybrid Journal   (Followers: 3)
Veterinary Record Open     Open Access   (Followers: 5)
Veterinary Research     Open Access   (Followers: 12)
Veterinary Research Communications     Hybrid Journal   (Followers: 3)
Veterinary Science Development     Open Access   (Followers: 1)
Veterinary Sciences     Open Access   (Followers: 3)
Veterinary Surgery     Hybrid Journal   (Followers: 26)
Wartazoa. Indonesian Bulletin of Animal and Veterinary Sciences     Open Access  
Zeitschrift für Ganzheitliche Tiermedizin     Hybrid Journal  
Zoonoses and Public Health     Hybrid Journal   (Followers: 4)

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Veterinary Pathology
Journal Prestige (SJR): 1.078
Citation Impact (citeScore): 2
Number of Followers: 21  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0300-9858 - ISSN (Online) 1544-2217
Published by Sage Publications Homepage  [1090 journals]
  • Image Challenge in Veterinary Pathology (Questions)
    • Pages: 465 - 466
      Abstract: Veterinary Pathology, Volume 57, Issue 4, Page 465-466, July 2020.

      Citation: Veterinary Pathology
      PubDate: 2020-07-01T10:13:24Z
      DOI: 10.1177/0300985820911454
      Issue No: Vol. 57, No. 4 (2020)
       
  • Image Challenge in Veterinary Pathology, Answers: Avian Diseases
    • Pages: 591 - 592
      Abstract: Veterinary Pathology, Volume 57, Issue 4, Page 591-592, July 2020.

      Citation: Veterinary Pathology
      PubDate: 2020-07-01T10:15:12Z
      DOI: 10.1177/0300985820911457
      Issue No: Vol. 57, No. 4 (2020)
       
  • Cellular Senescence: Mechanisms, Morphology, and Mouse Models
    • Authors: Jessica Beck, Izumi Horikawa, Curtis Harris
      Abstract: Veterinary Pathology, Ahead of Print.
      Cellular senescence is a cell cycle arrest in damaged or aged cells. Although this represents a critical mechanism of tumor suppression, persistence of senescent cells during aging induces chronic inflammation and tissue dysfunction through the adoption of the senescence-associated secretory phenotype (SASP). This has been shown to promote the progression of age-associated diseases such as Alzheimer’s disease, pulmonary fibrosis, and atherosclerosis. As the global population ages, the role of cellular senescence in disease is becoming a more critical area of research. In this review, mechanisms, biomarkers, and pathology of cellular senescence and SASP are described with a brief discussion of literature supporting a role for cellular senescence in veterinary diseases. Cell culture and mouse models used in senescence studies are also reviewed including the senescence-accelerated mouse—prone (SAMP), senescence pathway knockout mice (p53, p21 [CDKN1A], and p16 [CDKN2A]), and the more recently developed senolysis mice, which allow for direct visualization and elimination (or lysis) of senescent cells in live mice (p16-3MR and INK-ATTAC). These and other mouse models have demonstrated the importance of cellular senescence in embryogenesis and wound healing but have also identified a therapeutic benefit for targeting persistent senescent cells in age-associated diseases including neurodegeneration, diabetes, and cardiac fibrosis.
      Citation: Veterinary Pathology
      PubDate: 2020-08-03T09:32:36Z
      DOI: 10.1177/0300985820943841
       
  • Osteomalacia With Hyperostosis in Captive Lesser Hedgehog Tenrecs
           (Echinops telfairi)
    • Authors: Elise E. B. LaDouceur, Brian G. Murphy, Michael M. Garner, Andrew N. Cartoceti
      Abstract: Veterinary Pathology, Ahead of Print.
      Four captive, lesser hedgehog tenrecs (Echinops telfairi) were euthanized for soft bones that prevented normal mastication and/or ambulation. Antemortem radiographs (available in 2 cases) revealed osteopenia. Antemortem bloodwork (available in 2 cases) revealed hypophosphatemia. Dietary history (available in 2 cases) indicated the animals were eating only insects. Histologically, all examined bones had wide osteoid seams that caused expansion of the cortices. Osteoid deposition was exuberant and it partially filled marrow cavities (hyperostosis). Nondecalcified sections of bone (available in 2 cases) revealed that osteoid was poorly mineralized, consistent with osteomalacia. Insects are poor dietary sources of vitamin D, and dietary vitamin D deficiency is considered the most likely cause for metabolic bone disease in these animals.
      Citation: Veterinary Pathology
      PubDate: 2020-08-03T09:31:26Z
      DOI: 10.1177/0300985820943536
       
  • p53 Expression in Canine Liposarcoma Correlates With Myxoid Variant and
           Higher Proliferative Activity
    • Authors: Giancarlo Avallone, Luisa V. Muscatello, Alessandro Leoni, Paola Roccabianca, Elvio Lepri, Luca Crippa, Barbara Bacci
      Abstract: Veterinary Pathology, Ahead of Print.
      Canine liposarcoma is classified as well differentiated (WDL), dedifferentiated (DDL), myxoid (ML), and pleomorphic (PL). Overexpression of the protooncogene MDM2 has been reported in WDL and DDL, but little is known regarding the role of p53 in their tumorigenesis. The aim of this study was to assess p53 expression in canine liposarcoma and compare it with subtype, grade, mitotic count (MC), Ki67 labeling index (LI), and MDM2 expression. Forty-seven cases were included (13 WDL, 3 DDL, 7 ML, and 24 PL); 17 were MDM2-positive (13 WDL, 3DDL, and 1ML). Five were p53-positive (4 ML and 1 WDL) but DDL and PL were consistently negative. p53 expression correlated with higher Ki67-LI, higher MC, and myxoid histotype. No correlation was found with grade and MDM2 expression. Based on these results canine liposarcoma seems to embody a group of neoplasms whose subtypes, especially ML, may represent distinct diseases rather than morphological variants of the same entity.
      Citation: Veterinary Pathology
      PubDate: 2020-08-03T09:30:57Z
      DOI: 10.1177/0300985820941501
       
  • Pathology Associated With Streptococcus spp. Infection in Baboons (Papio
           spp.)
    • Authors: Katelin L. Davis, Olga Gonzalez, Shyamesh Kumar, Edward J. Dick
      Abstract: Veterinary Pathology, Ahead of Print.
      Streptococcus spp. are a source of morbidity and mortality in captive nonhuman primate populations. However, little is known about the lesions associated with naturally occurring streptococcal infections in baboons (Papio spp.). The pathology database of the Southwest National Primate Research Center was searched for all baboon autopsies from 1988 to 2018 in which Streptococcus spp. were cultured. Baboons on experimental protocol were excluded. The gross autopsy and histopathology reports were reviewed. Archived specimens were retrieved and reviewed as needed for confirmation or clarification. Fifty-six cultures were positive for Streptococcus spp. in 54 baboons with evidence of bacterial infection. Associated gross lesions included purulent exudate, fibrinous to fibrous adhesions, hemorrhage, mucosal thickening, organomegaly, and abscessation. Histologic lesions included suppurative inflammation, abscessation, necrosis, hemorrhage, fibrin accumulation, and thrombosis. Lungs and pleura (n = 31) were the most commonly infected organ followed by the central nervous system (n = 16), spleen (n = 15), soft tissues (n = 12), air sacs, liver, peritoneum, adrenal glands, heart, lymph nodes, uterus, kidneys, biliary system, bones, ears, umbilical structures, mammary glands, pancreas, placenta, and salivary glands. Infections by non-β-hemolytic Streptococcus spp. predominated in the lungs and air sacs; the most common isolate was Streptococcus pneumoniae. Infections by β-hemolytic Streptococcus spp. predominated in the soft tissues and reproductive tract. Naturally occurring β-hemolytic and non-β-hemolytic Streptococcus spp. infections cause morbidity and mortality in captive baboon populations. The lesions associated with streptococcal infection are similar to those reported in human infection. Thus, the baboon may represent an underutilized model for studying Streptococcus spp. as pathogens.
      Citation: Veterinary Pathology
      PubDate: 2020-08-03T09:28:30Z
      DOI: 10.1177/0300985820941496
       
  • Disease Progression in Lake Trout (Salvelinus namaycush) Experimentally
           Infected With Epizootic Epitheliotropic Disease Virus (Salmonid
           Herpesvirus-3)
    • Authors: Megan Shavalier, Mohamed Faisal, Thomas P. Loch, Scott D. Fitzgerald, Tuddow Thaiwong, Matti Kiupel
      Abstract: Veterinary Pathology, Ahead of Print.
      Epizootic epitheliotropic disease virus (salmonid herpesvirus-3; EEDV) is responsible for the death of millions of hatchery-raised lake trout (Salvelinus namaycush) in the Laurentian Great Lakes Basin. However, little is known about its biology, pathology, tropism, and host interactions. In this study, the presence and disease progression of EEDV were evaluated following exposure of naïve juvenile lake trout to EEDV via bath immersion under controlled laboratory conditions (n = 84 infected; n = 44 control). Individual tissues (n = 10 per fish), collected over 6 weeks, were analyzed for viral load by quantitative polymerase chain reaction, gross and histopathologic changes, and virus cellular targets using in situ hybridization. Skin, fin, and ocular tissues were the earliest viral targets and yielded the highest viral loads throughout the course of infection. Early gross lesions included exophthalmia, ocular hemorrhage, fin congestion, and hyperemia of visceral blood vessels. Advanced disease was characterized by multifocal to coalescing erosions and ulcerations of the skin, and congestion of visceral organs. Microscopically, there was cellular degeneration and necrosis in the epidermis and spleen, and lymphohistiocytic perivasculitis of the dermis, omentum, and the epicardium. EEDV DNA was first detected by in situ hybridization in epithelial cells of the epidermis, with subsequent labeling in the epithelial lining of primary and secondary gill lamellae. During advanced disease, EEDV was detected in endothelial and dendritic cells as well as blood monocytes. This study characterized EEDV tissue tropism and associated pathologic features, to guide research aimed at understanding EEDV disease ecology and improving strategies for disease control.
      Citation: Veterinary Pathology
      PubDate: 2020-08-03T09:25:46Z
      DOI: 10.1177/0300985820941268
       
  • AA Amyloid Deposition in the Central and Peripheral Nervous Systems in
           Flamingos
    • Authors: Ayumi Ono, Yumi Nakayama, Maki Inoue, Tokuma Yanai, Tomoaki Murakami
      Abstract: Veterinary Pathology, Ahead of Print.
      AA amyloidosis is characterized by amyloid deposition in systemic organs, but amyloid deposition in the central nervous system (CNS) or peripheral nervous system (PNS) is rare. In this study, AA amyloidosis was observed in 31 of 48 flamingos that died at a Japanese zoo. Almost all cases developed AA amyloidosis secondary to inflammatory diseases such as enteritis. Affected flamingos had AA amyloid deposition around blood vessels in periventricular white matter of the brain and in peripheral nerves. In addition, cerebral Aβ amyloidosis was observed in one of the 31 cases with AA amyloidosis. In conclusion, flamingos in the zoo commonly developed systemic amyloidosis with frequent amyloid deposition in the CNS and PNS, which seems to be a unique distribution in this avian species. Comparative pathological analyses in flamingos may help elucidate the pathogenesis of amyloid neuropathy.
      Citation: Veterinary Pathology
      PubDate: 2020-07-17T10:35:22Z
      DOI: 10.1177/0300985820939976
       
  • Clinical and Pathological Findings in SARS-CoV-2 Disease Outbreaks in
           Farmed Mink (Neovison vison)
    • Authors: Robert Jan Molenaar, Sandra Vreman, Renate W Hakze-van der Honing, Rob Zwart, Jan de Rond, Eefke Weesendorp, Lidwien A. M. Smit, Marion Koopmans, Ruth Bouwstra, Arjan Stegeman, Wim H. M. van der Poel
      Abstract: Veterinary Pathology, Ahead of Print.
      SARS-CoV-2, the causative agent of COVID-19, caused respiratory disease outbreaks with increased mortality in 4 mink farms in the Netherlands. The most striking postmortem finding was an acute interstitial pneumonia, which was found in nearly all examined mink that died at the peak of the outbreaks. Acute alveolar damage was a consistent histopathological finding in mink that died with pneumonia. SARS-CoV-2 infections were confirmed by detection of viral RNA in throat swabs and by immunohistochemical detection of viral antigen in nasal conchae, trachea, and lung. Clinically, the outbreaks lasted for about 4 weeks but some animals were still polymerase chain reaction–positive for SARS-CoV-2 in throat swabs after clinical signs had disappeared. This is the first report of the clinical and pathological characteristics of SARS-CoV-2 outbreaks in mink farms.
      Citation: Veterinary Pathology
      PubDate: 2020-07-14T04:30:26Z
      DOI: 10.1177/0300985820943535
       
  • Progressive Degenerative Myopathy and Myosteatosis in ASNSD1-Deficient
           Mice
    • Authors: Peter Vogel, Zhi-Ming Ding, Robert Read, Christopher M. DaCosta, Melissa Hansard, Daniel L. Small, Gui-lan Ye, Gwenn Hansen, Robert Brommage, David R. Powell
      Abstract: Veterinary Pathology, Ahead of Print.
      Mice with an inactivating mutation in the gene encoding asparagine synthetase domain containing 1 (ASNSD1) develop a progressive degenerative myopathy that results in severe sarcopenia and myosteatosis. ASNSD1 is conserved across many species, and whole body gene expression surveys show maximal expression levels of ASNSD1 in skeletal muscle. However, potential functions of this protein have not been previously reported. Asnsd1−/− mice demonstrated severe muscle weakness, and their normalized body fat percentage on both normal chow and high fat diets was greater than 2 SD above the mean for 3651 chow-fed and 2463 high-fat-diet–fed knockout (KO) lines tested. Histologic lesions were essentially limited to the muscle and were characterized by a progressive degenerative myopathy with extensive transdifferentiation and replacement of muscle by well-differentiated adipose tissue. There was minimal inflammation, fibrosis, and muscle regeneration associated with this myopathy. In addition, the absence of any signs of lipotoxicity in Asnsd1−/− mice despite their extremely elevated body fat percentage and low muscle mass suggests a role for metabolic dysfunctions in the development of this phenotype. Asnsd1−/− mice provide the first insight into the function of this protein, and this mouse model could prove useful in elucidating fundamental metabolic interactions between skeletal muscle and adipose tissue.
      Citation: Veterinary Pathology
      PubDate: 2020-07-08T10:08:14Z
      DOI: 10.1177/0300985820939251
       
  • E-Cadherin Expression in Canine Melanocytic Tumors: Histological,
           Immunohistochemical, and Survival Analysis
    • Authors: Serenella Silvestri, Ilaria Porcellato, Luca Mechelli, Laura Menchetti, Selina Iussich, Raffaella De Maria, Monica Sforna, Laura Bongiovanni, Chiara Brachelente
      Abstract: Veterinary Pathology, Ahead of Print.
      E-cadherin, a glycoprotein involved in cell-cell adhesion, has a pivotal role in epithelial-mesenchymal transition, a process through which neoplastic epithelial cells develop an invasive phenotype. In human cutaneous melanomas, decreased E-cadherin expression is associated with shorter survival and increased Breslow thickness, whereas in the dog its role is poorly understood. Tumor thickness and modified Clark level were recently proposed as useful features to assess canine melanocytic tumors, but no studies investigated their association with E-cadherin expression. We performed immunohistochemistry on 77 formalin-fixed, paraffin-embedded primary canine melanocytic tumors. A 3-tier and a 2-tier classification system for assessing E-cadherin expression were tested, with the latter being more informative for the assessment of canine melanocytic tumors. E-cadherin expression was lower in cutaneous melanomas than melanocytomas, as well as in amelanotic tumors compared to pigmented tumors. In amelanotic melanomas, absent E-cadherin expression was associated with an unfavorable outcome, suggesting a potential use of this marker in defining the prognosis of amelanotic melanomas. E-cadherin expression was lower in tumors with greater tumor thickness and modified Clark level ≥IV, suggesting its possible utility in identifying the most invasive tumors. The expression of E-cadherin in oral melanomas was heterogeneous, but was associated with pigmentation and clinical outcome; thus, E-cadherin evaluation could be advantageous to detect the most aggressive neoplasms. However, cutaneous melanomas without E-cadherin expression frequently had a favorable clinical outcome. Hence, its importance as prognostic factor should be carefully considered depending on the tumor origin.
      Citation: Veterinary Pathology
      PubDate: 2020-06-24T10:21:54Z
      DOI: 10.1177/0300985820934385
       
  • Polyneuropathy in Young Siberian Huskies Caused by Degenerative and
           Inflammatory Diseases
    • Authors: Hanne Jahns, Karen M. Vernau, Catherine M. Nolan, Emma J. O’Neill, Robert E. Shiel, G. Diane Shelton
      Abstract: Veterinary Pathology, Ahead of Print.
      Polyneuropathy is defined as the simultaneous dysfunction of several peripheral nerves. In dogs, a number of breeds are predisposed to a variety of immune-mediated and/or degenerative inherited forms of polyneuropathy, with laryngeal paralysis and/or megaesophagus as important clinical features of many of these conditions. This case series describes degenerative and inflammatory polyneuropathies in 7 young Siberian huskies that were categorized based on clinicopathological characteristics as follows: (1) slowly progressive laryngeal paralysis and megaesophagus caused by primary axonal degeneration with large fiber loss (n = 2); (2) slowly progressive polyneuropathy without megaesophagus or laryngeal paralysis caused by primary axonal degeneration with large fiber loss (n = 2); (3) acute inflammatory demyelinating neuropathy causing sensory, motor and autonomic nerve deficits (n = 2); and (4) ganglioradiculitis (sensory neuronopathy; n = 1). Based on the predominantly young age at onset, slow progression, relatedness of affected dogs, and clinical and pathological similarities with inherited neuropathies reported in other dog breeds, a hereditary basis for the degenerative polyneuropathies in Siberian huskies is suspected. However, 5 different mutations in 3 genes known to cause polyneuropathy in other dog breeds (NDRG1, ARHGEF10, or RAB3GAP1) were not detected in the affected Siberian huskies suggesting that more genetic variants remain to be identified. This study highlights the varied underlying lesions of polyneuropathies in young Siberian huskies.
      Citation: Veterinary Pathology
      PubDate: 2020-06-24T10:11:54Z
      DOI: 10.1177/0300985820934112
       
  • Pathology and Proteomics-Based Diagnosis of Localized Light-Chain
           Amyloidosis in Dogs and Cats
    • Authors: Ayumi Kadota, Susumu Iwaide, Shinya Miyazaki, Ikki Mitsui, Noboru Machida, Tomoaki Murakami
      Abstract: Veterinary Pathology, Ahead of Print.
      Amyloidosis is classified according to the amyloid precursor protein, and accurate diagnosis of the amyloidosis type may guide appropriate treatment. Immunohistochemistry and Congo red staining are the most frequently used methods used to distinguish types of amyloidosis, but problems with specificity and sensitivity indicate the need for an alternative diagnostic method. In this study, we evaluated laser microdissection-liquid chromatography-tandem mass spectrometry (LMD-LC-MS/MS) for the diagnosis of amyloid light-chain (AL) amyloidosis in animals. Plasmacytomas with amyloid deposits from 15 dogs and 2 cats were subjected to Congo red staining with or without potassium permanganate pretreatment, immunohistochemistry for kappa and lambda light chains, and LMD-LC-MS/MS. Congo red staining was diagnostic in 12 of 17 cases based on resistance to potassium permanganate pretreatment, but in 5 of 17 cases the pretreatment unexpectedly reduced Congo red staining or abrogated the birefringence and a definitive diagnosis could not be reached. Immunohistochemistry detected kappa or lambda light chains in 6 of 17 cases. With LMD-LC-MS/MS, immunoglobulin lambda light chain was detected in all 17 cases. The amyloid signature proteins ApoA-I, ApoA-IV, and ApoE were detected in 9, 1, and 3 of the 15 canine cases by LMD-LC-MS/MS, but not in the feline cases. In conclusion, LMD-LC-MS/MS consistently determined the amyloid type in all examined specimens, while Congo red staining after potassium permanganate treatment and immunohistochemistry were less sensitive tests.
      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:41:56Z
      DOI: 10.1177/0300985820934113
       
  • Diagnosis of Collagen Type III Glomerulopathy Using Picrosirius Red and
           PASH/Masson’s Trichrome Stain
    • Authors: Katelin L. Davis, Anne L. Burnum, Jessica A. Beck, Shannon G. M. Kirejczyk, Margaret A. Miller, Mario F. Sola, Cathy A. Brown
      Abstract: Veterinary Pathology, Ahead of Print.
      Canine collagen type III glomerulopathy (Col3GP) is a rare juvenile nephropathy in which irregular type III collagen fibrils and fibronectin accumulate in glomerular capillary walls and the mesangium. Necropsy findings were reviewed from 5 puppies diagnosed with Col3GP at 6 to 18 weeks of age. Histologically, with hematoxylin and eosin stain, the glomerular capillary walls and mesangium were diffusely and globally expanded by homogeneous pale eosinophilic material. Ultrastructurally, the subendothelial zone and mesangium were expanded by fibronectin and cross-banded collagen type III fibrils, diagnostic of Col3GP. Two additional stains were employed to identify the material within glomeruli as fibrillar collagen using light microscopy. In all 5 cases, the material was red with picrosirius red and birefringent under polarized light, and was blue with periodic acid-Schiff/hematoxylin/trichrome (PASH/TRI), thereby identifying it as fibrillar collagen. Based on these unique staining characteristics with picrosirius red and PASH/TRI, Col3GP may be reliably diagnosed with light microscopy alone.
      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:39:54Z
      DOI: 10.1177/0300985820934111
       
  • Cadaver, Carcase, or Carrion'
    • Authors: Orlando Paciello, Lorenzo Ressel
      Abstract: Veterinary Pathology, Ahead of Print.

      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:36:14Z
      DOI: 10.1177/0300985820932907
       
  • Carnivore Protoparvovirus-1 Associated With an Outbreak of Hemorrhagic
           Gastroenteritis in Small Indian Civets
    • Authors: Surangkanang Chaiyasak, Chutchai Piewbang, Wijit Banlunara, Somporn Techangamsuwan
      Abstract: Veterinary Pathology, Ahead of Print.
      Carnivore protoparvovirus-1 (CPPV-1) infection has been reported frequently in both domestic and wildlife species including wild carnivores. Fifty-five captive small Indian civets (Viverricula indica), farmed for perfume production in Eastern Thailand, showed clinical signs of acute bloody diarrhea, anorexia, vomiting, circling, and seizures. The disease spread within the farm and resulted in the death of 38 of the 55 civets (69% mortality) within a month. Fecal swabs were collected from the 17 surviving civets, and necropsy was performed on 7 of the dead civets. Pathologic findings were severe hemorrhagic gastroenteritis with generalized lymphadenopathy. CPPV-1 was identified in both fecal swabs and postmortem samples by species-specific polymerase chain reaction. Further whole-gene sequencing and restriction fragment length polymorphism analysis suggested feline panleukopenia virus (FPV) as the causative agent. The viral tropism and tissue distribution were confirmed by immunohistochemistry, with immunolabeling in the cytoplasm and nucleus of small intestinal crypt epithelial cells, villous enterocytes, histiocytes in lymphoid tissues, myenteric nerve plexuses, and cerebral and cerebellar neurons. Phylogenetic analysis of civet-derived CPPV-1 indicated a genetic similarity close to the FPV HH-1/86 strain detected in a jaguar (Panthera onca) in China. To our knowledge, this mass die-off of civets is the first evidence of disease associated with CPPV-1 infection in the subfamily Viverrinae. These findings support the multi-host range of parvovirus infection and raises awareness for CPPV-1 disease outbreaks in wildlife species.
      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:35:29Z
      DOI: 10.1177/0300985820932144
       
  • Alterations in Intestinal Innate Mucosal Immunity of Weaned Pigs During
           Porcine Epidemic Diarrhea Virus Infection
    • Authors: Ya-Mei Chen, Emma T. Helm, Nicholas Gabler, Jesse M. Hostetter, Eric R. Burrough
      Abstract: Veterinary Pathology, Ahead of Print.
      In the small intestine, localized innate mucosal immunity is critical for intestinal homeostasis. Porcine epidemic diarrhea virus (PEDV) infection induces villus injury and impairs digestive function. Moreover, the infection might comprise localized innate mucosal immunity. This study investigated specific enterocyte subtypes and innate immune components of weaned pigs during PEDV infection. Four-week-old pigs were orally inoculated with PEDV IN19338 strain (n = 40) or sham-inoculated (n = 24). At day post inoculation (DPI) 2, 4, and 6, lysozyme expression in Paneth cells, cellular density of villous and Peyer’s patch microfold (M) cells, and the expression of polymeric immunoglobulin receptor (pIgR) were assessed in the jejunum and ileum by immunohistochemistry, and interleukin (IL)-1β and tumor necrosis factor (TNF)-α were measured in the jejunum by ELISA. PEDV infection led to a decrease in the ratios of villus height to crypt depth (VH–CD) in jejunum at DPI 2, 4, and 6 and in ileum at DPI 4. The number of villous M cells was reduced in jejunum at DPI 4 and 6 and in ileum at DPI 6, while the number of Peyer’s patch M cells in ileum increased at DPI 2 and then decreased at DPI 6. PEDV-infected pigs also had reduced lysozyme expression in ileal Paneth cells at DPI 2 and increased ileal pIgR expression at DPI 4. There were no significant changes in IL-1β and TNF-α expression in PEDV-infected pigs compared to controls. In conclusion, PEDV infection affected innate mucosal immunity of weaned pigs through alterations in Paneth cells, villous and Peyer’s patch M cells, and pIgR expression.
      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:32:55Z
      DOI: 10.1177/0300985820932140
       
  • Canine Respiratory Coronavirus: A Naturally Occurring Model of
           COVID-19'
    • Authors: Simon L. Priestnall
      First page: 467
      Abstract: Veterinary Pathology, Ahead of Print.
      Discovered in 2003 at the Royal Veterinary College, London, canine respiratory coronavirus (CRCoV) is a betacoronavirus of dogs and major cause of canine infectious respiratory disease complex. Generally causing mild clinical signs of persistent cough and nasal discharge, the virus is highly infectious and is most prevalent in rehoming shelters worldwide where dogs are often closely housed and infections endemic. As the world grapples with the current COVID-19 pandemic, the scientific community is searching for a greater understanding of a novel virus infecting humans. Similar to other betacoronaviruses, SARS-CoV-2 appears to have crossed the species barrier, most likely from bats, clearly reinforcing the One Health concept. Veterinary pathologists are familiar with coronavirus infections in animals, and now more than ever this knowledge and understanding, based on many years of veterinary research, could provide valuable answers for our medical colleagues. Here I review the early research on CRCoV where seroprevalence, early immune response, and pathogenesis are some of the same key questions being asked by scientists globally during the current SARS-CoV-2 pandemic.
      Citation: Veterinary Pathology
      PubDate: 2020-05-05T04:31:01Z
      DOI: 10.1177/0300985820926485
       
  • Mission Accomplished: The ACVP/STP Coalition for Veterinary Pathology
           Fellows Completes Its Objectives, but Its Legacy and Spirit Live On
    • Authors: Rick Adler, Elizabeth Clark, Mark Cline, Michael Conner, Torrie Crabbs, Stacey Fossey, David Malarkey, Emily Meseck, William Vernau, Susan Tornquist, Kevin McDorman, Susan Rees, Gary Cockerell
      First page: 472
      Abstract: Veterinary Pathology, Ahead of Print.
      After 15 years of existence, the ACVP/STP Coalition for Veterinary Pathology Fellows will dissolve, primarily due to lack of renewed financial sponsorship. While in operation, the Coalition organized 32 new training position for veterinary pathologists, supported by $7.4 M from sponsors, including pharmaceutical and biotechnology companies, contract research organizations, private individuals and allied veterinary pathology support groups. All residual funds will be donated to ACVP and STP with the understanding that the two organizations will use these funds to enhance training by collaborating on outreach efforts, thus maintaining the legacy and spirit of the Coalition.
      Citation: Veterinary Pathology
      PubDate: 2020-04-23T05:01:53Z
      DOI: 10.1177/0300985820918314
       
  • Contagious Bovine Pleuropneumonia: A Comprehensive Overview
    • Authors: Giovanni Di Teodoro, Giuseppe Marruchella, Andrea Di Provvido, Anna Rita D’Angelo, Gianluca Orsini, Paola Di Giuseppe, Flavio Sacchini, Massimo Scacchia
      First page: 476
      Abstract: Veterinary Pathology, Ahead of Print.
      Contagious bovine pleuropneumonia (CBPP) is a respiratory disease of cattle that is listed as notifiable by the World Organization for Animal Health. It is endemic in sub-Saharan Africa and causes important productivity losses due to the high mortality and morbidity rates. CBPP is caused by Mycoplasma mycoides subsp. mycoides (Mmm) and is characterized by severe fibrinous bronchopneumonia and pleural effusion during the acute to subacute stages and by pulmonary sequestra in chronic cases. Additional lesions can be detected in the kidneys and in the carpal and tarsal joints of calves. Mmm infection occurs through the inhalation of infected aerosol droplets. After the colonization of bronchioles and alveoli, Mmm invades blood and lymphatic vessels and causes vasculitis. Moreover, Mmm can be occasionally demonstrated in blood and in a variety of other tissues. In the lung, Mmm antigen is commonly detected on bronchiolar and alveolar epithelial cells, in lung phagocytic cells, within the wall of blood and lymphatic vessels, inside necrotic areas, and within tertiary lymphoid follicles. Mmm antigen can also be present in the cytoplasm of macrophages within lymph node sinuses, in the germinal center of lymphoid follicles, in glomerular endothelial cells, and in renal tubules. A complete pathological examination is of great value for a rapid presumptive diagnosis, but laboratory investigations are mandatory for definitive diagnosis. The purpose of this review is to describe the main features of CBPP including the causative agent, history, geographic distribution, epidemiology, clinical course, diagnosis, and control. A special focus is placed on gross and microscopic lesions in order to familiarize veterinarians with the pathology and pathogenesis of CBPP.
      Citation: Veterinary Pathology
      PubDate: 2020-05-11T10:27:59Z
      DOI: 10.1177/0300985820921818
       
  • Giant Cell Sarcomas in Domestic Rabbits (Oryctolagus cuniculus)
    • Authors: Christof A. Bertram, Michael M. Garner, Drury Reavill, Robert Klopfleisch, Matti Kiupel
      First page: 490
      Abstract: Veterinary Pathology, Ahead of Print.
      Multinucleated giant cells (MGCs) are a prominent histological feature of various mesenchymal neoplasms and are often considered a criterion of malignancy. Mesenchymal neoplasms with MGCs for which the cell lineage is unclear generally are referred to as giant cell sarcomas. Here we characterize the gross, histologic, and immunohistochemical features of 90 giant cell sarcomas in domestic pet rabbits. Based on the anatomic location and histologic and immunohistochemical findings, 18 cases were classified as histiocytic sarcomas (HS) and 72 cases as anaplastic sarcomas (AS). At postmortem examination, HS was either localized HS (n = 7) always affecting the lungs, or disseminated HS (n = 10) that affected the lungs (n = 10), liver (n = 6), kidneys (n = 4), pleura (n = 2), mediastinum (n = 2), heart (n = 4), skeletal muscle (n = 1), adipose tissue (n = 1), and lymph node (n = 1). Additionally, one cecal biopsy was consistent with HS. Microscopically, HS were characterized by sheets of neoplastic polygonal to round cells that contained single to several, often greatly enlarged nuclei as well as abundant cytoplasm. HS were always positive for CD204 and always negative for SMA and desmin. In contrast, AS arose most commonly from the skin or subcutis (n = 62) and rarely the skeletal muscle (n = 8) or abdominal organs (n = 2). In 29% of extra-abdominal AS, the tumor deeply invaded into surrounding connective tissue, skeletal muscle, tendons, and bone causing pathological fractures. Five of 9 postmortem cases metastasized to various organs often including the lungs. Microscopically, AS were characterized by sheets of spindle or pleomorphic cells admixed with variable numbers of MGCs. Immunohistochemically, AS were always negative for CD204 and often (71%) positive for SMA and/or desmin.
      Citation: Veterinary Pathology
      PubDate: 2020-04-29T10:46:12Z
      DOI: 10.1177/0300985820921814
       
  • Foxp3+ Regulatory T Cells Associated With CCL17/CCR4 Expression in
           Carcinomas of Dogs
    • Authors: Shingo Maeda, Maho Nakazawa, Mona Uchida, Ryohei Yoshitake, Takayuki Nakagawa, Ryohei Nishimura, Ryo Miyamoto, Makoto Bonkobara, Tomohiro Yonezawa, Yasuyuki Momoi
      First page: 497
      Abstract: Veterinary Pathology, Ahead of Print.
      Regulatory T cells (Tregs) can be targeted in cancer immunotherapy. A previous study has shown that the chemokine CCL17 and the receptor CCR4 play a role in Treg recruitment in canine urothelial carcinoma. Here, we describe the association of tumor-infiltrating Tregs with CCL17/CCR4 expression in dogs with other carcinomas. In this study, we investigated 23 dogs with mammary carcinoma, 14 dogs with oral squamous cell carcinoma, 16 dogs with pulmonary adenocarcinoma, and 8 healthy control dogs. Immunohistochemistry showed that Foxp3+ Tregs and CCR4+ cells were increased in the tumor tissues of mammary carcinoma, squamous cell carcinoma, and pulmonary adenocarcinoma, when compared with the healthy tissues. The number of CCR4+ cells was associated with that of Foxp3+ Tregs. Double immunofluorescence labeling confirmed that most tumor-infiltrating Foxp3+ Tregs expressed CCR4. In vitro, canine carcinoma cell lines expressed CCL17 mRNA. Quantitative RT-PCR (reverse transcriptase-polymerase chain reaction) showed that CCL17 mRNA expression in canine carcinomas was increased approximately 10- to 25-fold relative to that of healthy tissues. These results suggest that the CCL17/CCR4 axis may drive Treg recruitment in a variety of canine carcinomas. CCR4 blockade may be a potential therapeutic option for tumor eradication through Treg depletion.
      Citation: Veterinary Pathology
      PubDate: 2020-04-29T10:41:39Z
      DOI: 10.1177/0300985820921535
       
  • Expression and Prognostic Significance of Neurotrophins and Their
           Receptors in Canine Mammary Tumors
    • Authors: Bernadette Rogez, Quentin Pascal, Audrey Bobillier, François Machuron, Robert-Alain Toillon, Dominique Tierny, Valérie Chopin, Xuefen Le Bourhis
      First page: 507
      Abstract: Veterinary Pathology, Ahead of Print.
      Accumulating data highlight the role of neurotrophins and their receptors in human breast cancer. This family includes nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), both synthetized as proneurotrophins (proNGF and proBDNF). (pro)NGF and (pro)BDNF initiate their biological effects by binding to both their specific receptors TrkA and TrkB, respectively, and the common receptor p75NTR. Currently, no data are available about their expression and potential role in canine mammary tumors. The aim of this study was to investigate expression of proNGF and BDNF as well as their receptors TrkA, TrkB, and p75NTR in canine mammary carcinomas, and to correlate them with clinicopathological parameters (grade, histological type, lymph node status, recurrence, and distant metastasis) and survival. Immunohistochemistry was performed on serial sections of 96 canine mammary carcinomas with antibodies against proNGF, BDNF, TrkA, TrkB, and p75NTR. Of the 96 carcinomas, proNGF expression was detected in 71 (74%), BDNF in 79 (82%), TrkA in 94 (98%), TrkB in 35 (37%), and p75NTR in 44 (46%). No association was observed between proNGF, BDNF, or TrkA expression and either clinicopathological parameters or survival. TrkB and p75NTR expression were associated with favorable clinicopathological parameters as well as better overall survival.
      Citation: Veterinary Pathology
      PubDate: 2020-04-30T10:19:58Z
      DOI: 10.1177/0300985820921813
       
  • Long-Term Observation of the Progression From Nodal Marginal Zone Lymphoma
           to Diffuse Large B-Cell Lymphoma in a Dog
    • Authors: Takanori Shiga, James K. Chambers, Mei Sugawara, Yuko Goto-Koshino, Hajime Tsujimoto, Hiroyuki Nakayama, Kazuyuki Uchida
      First page: 520
      Abstract: Veterinary Pathology, Ahead of Print.
      A 4-year and 10-month old female Pembroke Welsh Corgi presented with an enlarged right popliteal lymph node, and a histopathological diagnosis of nodal marginal zone lymphoma (nMZL) was made. After resection of the lymph node, follow-up observation was continued without chemotherapy. At 22 months after initial presentation, the dog developed enlargement of peripheral lymph nodes, and the histopathological diagnosis was late-stage nMZL. Multidrug chemotherapy induced clinical complete remission, but the tumor relapsed with enlargement of peripheral and abdominal lymph nodes 42 months after initial presentation. Second-round multidrug chemotherapy induced complete clinical remission again; however, the tumor relapsed with lymphadenopathy 47 months after initial presentation. The dog died 59 months after initial presentation, and postmortem examination revealed generalized lymphadenopathy; the histopathological diagnosis was diffuse large B-cell lymphoma (DLBCL). Polymerase chain reaction for antigen receptor gene rearrangements revealed that the nMZL and DLBCL samples were derived from the same B-lymphocyte clone.
      Citation: Veterinary Pathology
      PubDate: 2020-06-17T11:33:48Z
      DOI: 10.1177/0300985820932143
       
  • Equine Hoof Canker: Bovine Papillomavirus Infection Is Not Associated With
           Impaired Keratinocyte Differentiation
    • Authors: Veronika Apprich, Theresia Licka, Sabrina Freiler, Cordula Gabriel
      First page: 525
      Abstract: Veterinary Pathology, Ahead of Print.
      Impaired keratinocyte differentiation has recently been suggested as a key event in equine hoof canker development. Koilocytotic appearance of keratinocytes, one of the most characteristic morphological alterations in hoof canker tissue, is also a common marker for papillomavirus (PV) infection, and bovine PV-1 and/or -2 (BPV-1/2) has previously been detected in equine canker patients. Therefore, the present study aimed to correlate the frequency and severity of koilocytotic keratinocytes with BPV detection in hoof canker samples. Hoof tissue of 5/18 canker-affected horses and 2/6 control horses tested positive for BPV-1/2 DNA using polymerase chain reaction. Thus, no association between the presence of BPV-1/2 papillomaviral DNA and koilocytotic appearance was found. Proteins associated with but not specific for PV infection were also investigated. Using immunohistochemistry, specific adhesion molecules (E-cadherin and β-catenin) and intermediate filaments (keratins 6 and 14) important for intact epidermal barrier function and keratinocyte differentiation were documented in control samples (n = 6) and in hoof canker tissue samples (n = 19). Altered expression patterns of intermediate filaments and adhesion molecules were demonstrated in canker tissue, confirming the importance of incomplete keratinocyte differentiation, as well as the crucial role of keratinocyte differentiation in hoof canker.
      Citation: Veterinary Pathology
      PubDate: 2020-04-29T10:47:59Z
      DOI: 10.1177/0300985820921820
       
  • Characterization of Fetal Brain Damage in Early Abortions of Ovine
           Toxoplasmosis
    • Authors: Daniel Gutiérrez-Expósito, Noive Arteche-Villasol, Raquel Vallejo-García, María C. Ferreras-Estrada, Ignacio Ferre, Roberto Sánchez-Sánchez, Luis Miguel Ortega-Mora, Valentín Pérez, Julio Benavides
      First page: 535
      Abstract: Veterinary Pathology, Ahead of Print.
      There is an unacknowledged clinical presentation of ovine toxoplasmosis characterized by early abortions and lesions of fetal leukoencephalomalacia. To investigate the pathogenesis of this condition, the extent and distribution of leukomalacia and the variations in the cell populations associated with it were characterized in 32 fetal brains from 2 previously published experimental studies of Toxoplasma gondii infection in pregnant sheep. Immunohistochemical labeling of βAPP allowed for the detection of leukomalacia in 100/110 (91%) studied samples. There was no clear influence of the challenge dose or the area of the brain (frontal lobe, corpus callosum, midbrain, and cerebellum). In tissues with leukomalacia, there was loss of oligodendrocytes and increased number of astrocytes and microglia both in the areas of necrosis but also in the surrounding area. These findings were similar to those described in ovine experimental models (inflammation syndrome and hypoxic models) of periventricular leukomalacia in humans. Thus, a fetal inflammatory syndrome may be involved in the pathogenesis of early abortion in ovine toxoplasmosis. However, further studies are needed to determine the pathogenesis of this clinical presentation because placental thrombosis and resulting hypoxia could also be responsible for the leukomalacia.
      Citation: Veterinary Pathology
      PubDate: 2020-05-14T10:35:19Z
      DOI: 10.1177/0300985820921539
       
  • Macrophages and T Lymphocytes in the Ovine Placenta After Experimental
           Infection With Toxoplasma gondii
    • Authors: Pablo Castaño, Miguel Fuertes, Miguel Fernández, M. Carmen Ferreras, Ignacio Ferre, Luis Miguel Ortega-Mora, Valentín Pérez, Julio Benavides
      First page: 545
      Abstract: Veterinary Pathology, Ahead of Print.
      Early abortion in ovine toxoplasmosis has had limited investigation. This study evaluated the immune response in the placenta of sheep orally infected with Toxoplasma gondii and euthanized between 2 and 4 weeks postinfection. Toxoplasma infection of the placenta was only found at 4 weeks after infection. Parasitic debris in foci of necrosis were immunolabeled in the maternal caruncle, whereas well-preserved intracellular parasitic vacuole-like structures were found in trophoblasts of fetal cotyledon. Early abortions had increased macrophages in caruncular septa, whereas in later abortions the placentas containing the parasite had an increase of T lymphocytes and macrophages mainly in the fetal cotyledons. This study suggests that the immune response in both the fetal and maternal compartments of the placenta may contribute to the pathogenesis of ovine toxoplasmosis and that these responses differ between early and late presentations of the disease.
      Citation: Veterinary Pathology
      PubDate: 2020-05-26T12:07:47Z
      DOI: 10.1177/0300985820923987
       
  • Peste Des Petits Ruminants Virus and Goatpox Virus Co-Infection in Goats
    • Authors: Olatunde Babatunde Akanbi, Kati Franzke, Adeyinka Jeremy Adedeji, Reiner Ulrich, Jens Peter Teifke
      First page: 550
      Abstract: Veterinary Pathology, Ahead of Print.
      Infection of small ruminants with peste des petits ruminants virus (PPRV) and goatpox virus (GTPV) are endemic and can have devastating economic consequences in Asia and Africa. Co-infection with these viruses have recently been reported in goats and sheep in Nigeria. In this study, we evaluated samples from the lips of a red Sokoto goat, and describe co-infection of keratinocytes with PPRV and GTPV using histopathology and transmission electron microscopy. Eosinophilic cytoplasmic inclusion bodies were identified histologically, and ultrastructural analysis revealed numerous large cytoplasmic viral factories containing poxvirus particles and varying sizes of smaller cytoplasmic inclusions composed of PPRV nucleocapsids. These histopathological and ultrastructural findings show concurrent infection with the 2 viruses for the first time as well as the detection of PPRV particles in epithelial cells of the mucocutaneous junction of the lip.
      Citation: Veterinary Pathology
      PubDate: 2020-05-26T12:12:24Z
      DOI: 10.1177/0300985820926954
       
  • Synovial Cysts and Myxomas in 16 Cats
    • Authors: Linden E. Craig, Paula M. Krimer, Alicia D. O’Toole
      First page: 554
      Abstract: Veterinary Pathology, Ahead of Print.
      This report describes the clinical and pathologic characteristics of cystic and myxomatous lesions of synovial joints in 16 cats. The average age was 13.4 years. The elbow was most commonly affected (12/16), and all lesions were unilateral. Degenerative joint disease was a frequent concurrent but bilateral condition. The lesions consisted of fluid-filled cysts lined by synoviocytes (3 cases), solid foci of stellate cells in a myxomatous matrix (2 cases), or a combination of the two (11 cases). In some cases there were areas of transition between the cystic and myxomatous lesions. Mitoses and other features of malignancy were rare to nonexistent. In the 13 cats with follow-up information, the lesion gradually increased in size over a period of years. None of the cats died or were euthanized because of this lesion. We propose that some cats with degenerative joint disease develop synovial cysts, which have the potential to transform to a synovial myxoma.
      Citation: Veterinary Pathology
      PubDate: 2020-05-21T01:40:43Z
      DOI: 10.1177/0300985820926483
       
  • Feline Niemann-Pick Disease With a Novel Mutation of SMPD1 Gene
    • Authors: Yuta Takaichi, James K. Chambers, Mun Keong Kok, Hiroki Uchiyama, Makoto Haritani, Daisuke Hasegawa, Hiroyuki Nakayama, Kazuyuki Uchida
      First page: 559
      Abstract: Veterinary Pathology, Ahead of Print.
      A 4-month-old female mixed-breed cat showed gait disturbance and eventual dysstasia with intention tremor and died at 14 months of age. Postmortem histological analysis revealed degeneration of neuronal cells, alveolar epithelial cells, hepatocytes, and renal tubular epithelial cells. Infiltration of macrophages was observed in the nervous system and visceral organs. The cytoplasm of neuronal cells was filled with Luxol fast blue (LFB)-negative and periodic acid-Schiff (PAS)-negative granules, and the cytoplasm of macrophages was LFB-positive and PAS-negative. Ultrastructurally, concentric deposits were observed in the brain and visceral organs. Genetic and biochemical analysis revealed a nonsense mutation (c.1017G>A) in the SMPD1 gene, a decrease of SMPD1 mRNA expression, and reduced acid sphingomyelinase immunoreactivity. Therefore, this cat was diagnosed as having Niemann-Pick disease with a mutation in the SMPD1 gene, a syndrome analogous to human Niemann-Pick disease type A.
      Citation: Veterinary Pathology
      PubDate: 2020-04-29T10:43:40Z
      DOI: 10.1177/0300985820921810
       
  • Enterospora nucleophila (Microsporidia) in Gilthead Sea Bream (Sparus
           aurata): Pathological Effects and Cellular Immune Response in Natural
           Infections
    • Authors: Amparo Picard-Sánchez, M. Carla Piazzon, Nahla Hossameldin Ahmed, Raquel Del Pozo, Ariadna Sitjà-Bobadilla, Oswaldo Palenzuela
      First page: 565
      Abstract: Veterinary Pathology, Ahead of Print.
      Enterospora nucleophila is a microsporidian responsible for an emaciative disease in gilthead sea bream (Sparus aurata). Its intranuclear development and the lack of in vitro and in vivo models hinder its research. This study investigated the associated lesions, its detection by quantitative polymerase chain reaction, and the cellular immune response of naturally infected fish. The intensity of infection in the intestine was correlated with stunted growth and reduced body condition. At the beginning of the outbreaks, infection prevalence was highest in intestine and stomach, and in subsequent months, the prevalence decreased in the intestine and increased in hematopoietic organs and stomach. In heavy infections, the intestine had histologic lesions of enterocyte hypercellularity and proliferation of rodlet cells. Infected enterocytes had E. nucleophila spores in the cytoplasm, and a pyknotic nucleus, karyorhexis or karyolysis. Lymphocytes were present at the base of the mucosa, and eosinophilic granule cells were located between the enterocytes. In intestinal submucosa, macrophage aggregates containing spores were surrounded by lymphocytes and granulocytes, with submucosal infiltration of granulocytes. Macrophage aggregates appeared to develop into granulomata with necrotic areas containing parasite remnants. Immunohistochemistry revealed mast cells as the main type of granulocyte involved. Abundant IgM+ and IgT+ cells were identified by in situ hybridization in the submucosa when intracytoplasmic stages were present. This study describes the lesions of E. nucleophila in gilthead sea bream, an important aquaculture species.
      Citation: Veterinary Pathology
      PubDate: 2020-06-12T08:04:15Z
      DOI: 10.1177/0300985820927707
       
  • Ibex-Associated Malignant Catarrhal Fever in Duikers (Cephalophus Spp)
    • Authors: Francisco R. Carvallo, Francisco A. Uzal, Janet D. Moore, Kenneth Jackson, Akinyi C. Nyaoke, Lisa Naples, Jordan Davis-Powell, Cynthia K. Stadler, Brandon A. Boren, Cristina Cunha, Hong Li, Patricia A. Pesavento
      First page: 577
      Abstract: Veterinary Pathology, Ahead of Print.
      Eight duikers, representing 3 different species cohoused in a single zoological collection, died in a 10-month period. Black, red-flanked, and yellow-backed duikers were affected, appearing clinically with a combination of anorexia, diarrhea, ataxia, tremors, and/or stupor, followed by death within 72 hours of onset of clinical signs. Consistent gross findings were pulmonary ecchymoses (8/8), generalized lymphadenomegaly (6/8), ascites (5/8), and pleural effusion (4/8). Dense lymphocyte infiltrates and arteritis affected numerous tissues in most animals. Ibex-associated malignant catarrhal fever (MCF) viral DNA was detected in all cases by polymerase chain reaction and in situ hybridization. Identical ibex-MCF virus sequence was detected in spleen of a clinically healthy ibex (Capra ibex) housed in a separate enclosure 35 meters away from the duikers.
      Citation: Veterinary Pathology
      PubDate: 2020-05-14T10:33:20Z
      DOI: 10.1177/0300985820918313
       
  • Herpesvirus Encephalitis in a Little Blue Penguin (Eudyptula minor)
    • Authors: Kathryn A. Tuxbury, Charles J. Innis, Tuddow Thaiwong, Annabel G. Wise, Roger Maes, Michael M. Garner, Matti Kiupel
      First page: 582
      Abstract: Veterinary Pathology, Ahead of Print.
      An 11-day-old little blue penguin (Eudyptula minor) died unexpectedly. Prior to hatching, the egg experienced trauma and resultant defects were repaired. The chick hatched without complication and was clinically normal prior to death. Necropsy revealed congested lungs. Histologic examination showed moderate nonsuppurative encephalitis with focally extensive neuronal necrosis and intranuclear inclusions in neurons within necrotic foci. Herpesvirus DNA was detected in brain tissue with a generic herpesvirus polymerase chain reaction. Sanger sequencing demonstrated 100% and 98% sequence homology to sphenicid alphaherpesvirus 1 and penguin herpesvirus 2, respectively. In situ hybridization demonstrated large amounts of herpesvirus nucleic acid in intranuclear inclusions and neuronal nuclei. Combined histology, polymerase chain reaction, Sanger sequencing, and in situ hybridization results were most consistent with herpesviral encephalitis, most likely caused by sphenicid alphaherpesvirus 1. To our knowledge, this is the first report of a herpesvirus infection causing encephalitis in a penguin and the first report of herpesvirus in this species.
      Citation: Veterinary Pathology
      PubDate: 2020-05-21T01:42:43Z
      DOI: 10.1177/0300985820926678
       
  • Persistent Corynebacterium bovis Infectious Hyperkeratotic Dermatitis in
           Immunocompetent Epidermal-Mutant dep/dep Mice
    • Authors: Emily L. Miedel, Natalie H. Ragland, Andrea R. Slate, Robert W. Engelman
      First page: 586
      Abstract: Veterinary Pathology, Ahead of Print.
      During a previously reported program-wide Corynebacterium bovis outbreak, both immunocompetent depilated (dep/dep) mutant mice and transgenic mice that express the papillomavirus E6 oncoprotein became persistently infected with C. bovis. An orthokeratotic, hyperkeratotic, acanthotic dermatitis developed in the C. bovis–infected dep/dep mice, which remained C. bovis PCR-positive for>45 days prior to euthanasia as part of the program-wide C. bovis eradication effort. Since both affected strains of mice have altered skin homeostasis, immune status or the presence of hair may not alone be sufficient to explain strain susceptibility to C. bovis–related cutaneous disease. In order to avoid invalidation of preclinical studies due to C. bovis infection, it may be necessary to isolate immunodeficient mouse strains, implement facililty-wide surveillance for C. bovis, and sterilize equipment with vaporized hydrogen peroxide.
      Citation: Veterinary Pathology
      PubDate: 2020-04-29T10:49:41Z
      DOI: 10.1177/0300985820922219
       
  • Book Review: The Illustrated Dictionary of Toxicologic Pathology and
           Safety Science
    • Authors: Judit E. Markovits
      First page: 590
      Abstract: Veterinary Pathology, Ahead of Print.

      Citation: Veterinary Pathology
      PubDate: 2020-05-14T10:35:59Z
      DOI: 10.1177/0300985820924393
       
 
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