Abstract: Fifty-six Hyla breeds of 12-week-old rabbit bucks were randomly distributed across 7 diet groups comprising the control, 0.25% nutmeg seed meal (NSM), 0.50%, 0.25% clove leaf meal (CLM), 0.50% CLM, 0.25% composite nutmeg and clove leaf meal mix (CNCM), and 0.50%CNCM. At the end of the 8 weeks of the experiment, 3 bucks per diet group were sacrificed. The growth performance, haematology, serum biochemical indices, and testicular morphometry of the buck were assessed. The different diet groups significantly increased (P<0.05) the feed intake, weight gained, and feed conversion ratio compared to the control. The highest weight gained and best feed conversion ratio were observed among bucks fed 0.25% CNCM. Dietary supplementation of NSM, CLM, and CLCM at 0.25 and 0.50% levels did not affect (P>0.05) the blood parameters, serum proteins, enzymes, and metabolites except for serum glutathione and catalase activity which revealed that the phytochemicals in NSM and CNCM could effectively combat free scavenging radicals. Testicular weight and densities were positively influenced (P<0.05) by dietary supplements which are indicative of better testicular functions compared to the control. Hence, the synergetic effect of NSM and CLM at 0.25% supplementation level could be incorporated into Bucks’ diet for better productivity. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: The aim of this study was to estimate the effect of feeding adult Awassi rams different ratio of dried olive leaves on the reproductive parameters during seven weeks during the breeding season. Three groups of Awassi rams were fed for twelve weeks either a conventional wheat straw-concentrate ration (Control) or experimental rations where 30% (G1) and 60% (G2) of the wheat straw were replaced with dried olive leaves. Animals were fed the same amounts of crude protein and metabolizable energy daily. Results indicated that with the exception of scrotal circumference, no significant (p>0.05) responses were noted for testosterone level, body weight, semen volume, sperm concentration or semen pH values between the tested groups. Analysis of motility characteristics of sperm by computer-assisted semen analysis (CASA) technique showed no significant differences (p>0.05) between the three groups in the percent motility, percent of sperm showing progressive motility, average path velocity, straight line velocity, curvilinear velocity, percent straightness and percent linearity. It could be concluded that feeding Awassi rams dried olive leaves even at the 60% level did not affect the reproductive characteristics of the Awassi rams and therefore could be used as supplement sources of feeds for small ruminants. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: This study was conducted at the ruminant station of the Technical Institute of Breeding Baba-Ali on the peri-partum period in ewes of the Rembi breed. It focused on the variations of some hematological and biochemical parameters of 7 ewes during 2 months (1 month before lambing and one month after). The blood samples were taken every 15 days, which were analyzed by blood count within the hour following the blood sampling, concerning the hematological parameters. Besides, for the biochemical parameters, the blood was centrifuged and the sera were frozen until the day of analysis. The results of the analysis using logicial R revealed nonsignificant variations (P >0.05) for the following parameters: White blood cells, Lymphocytes, Red blood cells, Hemoglobin, Hematocrit, and Blood platelets. These results are contradictory to the results obtained in previous studies except for a study conducted on ewes of the Lacaune breed. Regarding the biochemical parameters, the variation was significant (P<0.05) for cholesterol, urea, and creatinine, but not significant for calcium, glucose and triglycerides (P >0.05). The results obtained are debatable from the results quoted by several authors, except for creatinine which was found to be much higher. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: In Romania, the livestock is very scattered on the diverse environmental conditions. Due to difficulties regarding data collection, with the help of genomic selection, the paradigm of genetic improvement of economical traits in Romanian cattle breeds can be changed. Population studied in this paper consist in animals genotyped using 60k SNP panel. Association studies were performed for estimate the effect of SNP polymorphism on milk yield at 305 days. Genetic evaluation was compared under four statistical methodologies were compared under six scenarios 5k, 10k, 15k, 20k, 25k and 37k SNP panels. High association with phenotypes were displayed by 38 SNP’s. Generally, accuracy of the studied models, tend to increase with complexity (informational input). The model with the lowest accuracy was Sire-Model (36.6% accuracy). Adding information in Animal Model increase accuracy of estimation and finally, adding genomic information the accuracy was maximized to 69.9%. For each lactation all the statistical models return highly correlated breeding values. The highest (97.2%) and the lowest (79.2%) rank correlation are registered between Test-Day and Animal Model for the first and second lactation. Genomic evaluation is a more feasible methodology than classical breeding value estimation, in term of accuracy of selection and the quality of the parentage information. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: Initially, the nutritional value of the three main by-products of Cannabis sativa (hempseed cake, second-rate quality hemp seed and hemp hay), cultivated under Greek conditions and collected from four plantations in the Macedonian region was estimated. A second part of the study was the investigation of the effects of diet inclusion with hempseed cake (HSC) on the performance of Holstein dairy cows. The HSC used for this experiment was the one analyzed in the first part of the study. A total number of 20 lactating cows were allocated into two equal groups in a randomized block design. Cows of both groups were offered a total mixed ration (TMR) on the same feed allowance. The diet of the experimental group was formulated with 3.5 % hemp cake, at a quantity of 1kg of hemp cake per cow per day. Milk yield was recorded individually, and feed refusals were recorded on a pen basis daily, during the first 40 days of lactation. Individual milk samples were analyzed for their chemical composition, lipid oxidative stability and fatty acid composition and tetrahydrocannabinol (THC) and cannabidiol (CBD) content, as well as feed samples of each group. Individual blood samples were received for biochemical indices assessment. All data were subjected ANOVA statistical analysis. The results of hemp by-products chemical profile showed similar nutritional values among the different cultivation regions. The experimental results indicated that the inclusion of HSC did not affect milk production and composition (P > 0.05). However, diet inclusion with HSC favorably influenced milk fatty acid profile. Finally, serum NEFA concentration was lower for the HSC group while serum urea levels were higher (P < 0.05). PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: Antibiotics and zinc oxide are therapeutic agents widely used in the livestock industries, reducing significantly the negative effects of weaning by improving the growth rate and decreasing mortality. Although, due to the potential public and environmental risks associated with antibiotic resistance and soil pollution their use in the E.U. has been drastically reduced or banned, thus, alternative solutions been necessary to be develop. The addition of polyphenols to animal feed has shown promising results, as they have a broad antimicrobial activity including against E. coli F4 (K88). Moreover, it has been observed to hold a wide range of beneficial properties such as antidiarrhoeic, anti-inflammatory, and antioxidant activity. In the present study in vitro experiments were performed in order to investigate the antimicrobial activity of several polyphenolic extracts obtained from mustard seed meals of two origins (Poland and Moldavia), grape seed meal, sea buckthorn seed meal, rapeseed meal, and rapeseed meal fermented with S. cerevisiae, against E. coli F4 (K88) proliferation measured at different intervals for 24 hours. The results revealed that polyphenols have a considerable antimicrobial effect in high concentrations. The inhibition was dependent also on polyphenol composition and even the origin place. Withal, it was observed that the polyphenols extracted from rapeseed meal and fermented rapeseed meal presented noticeable differences in inhibition, in similar concentrations, this suggesting that the fermentation process considerably affects the profile of rapeseed meal polyphenols. Likewise for the Moldavian mustard seed extract which significantly inhibited bacterial growth from very low concentrations although it had a low concentration of total polyphenols. Thus, the polyphenolic content of fermented rape seed meal extract presented an enhanced antimicrobial activity against E. coli F4 (K88) compared with the non-fermented rape seed meal extract. Although the total polyphenol concentration was lower in FRSM than in RSM. Polyphenols extracted from Moldavia mustard seed meal exhibited remarkable efficacy in inhibiting bacterial growth. Notably, even at a concentration as low as 3.71 µg/ml, the polyphenols demonstrated significant antimicrobial activity, effectively combating bacterial development. Overall, our study demonstrated that polyphenols represent a promising substitute for antibiotics and zinc oxide and might represent an encouraging long-term solution in overcoming the weaning crisis in pigs. In vivo experimentations are needed to validate these results. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: This research was carried out to evaluate the influence of cage tiers height on cloaca temperature, performance and egg quality of Isa-Brown laying birds. A total of One hundred and eight (108) Isa-Brown laying birds were selected from a healthy stock. The poultry house contained conventional battery cages arranged in a three-tier design. One unit of the battery cage made up of three (3) tiers with three (3) cells per tier was setup to accommodate three (3) birds per cell and a total of 27 birds per unit cage. This setup was replicated for three additional cage units. The performance of the birds was evaluated in terms of the egg weight, egg mass, feed conversion ratio, hen day production, and total feed consumed. The results revealed no significant (P >0.05) difference among cage tiers in terms of egg weight and feed conversion ratio but egg mass, hen day production and total feed consumed were significantly influenced. Highest egg mass (38.96g), highest Hen Day production (78.66%) and highest daily feed intake (99.01g) were recorded in birds raised in the upper tier of the cage. For the period of the study, cloaca temperature ranged between 36 °C and 41 °C. Cloaca temperature was not significantly (P >0.05) affected by cage tiers height. In comparison to layers raised in low-tier cages, those raised in high-tier cages had higher egg production and better feed efficiency. These findings suggest that cage tier heights play an important role in the production performance of laying hens but cloaca temperature was not affected. However, further researches on the effect of cage tiers height on cloaca temperature should be carried out in a controlled setting PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: The study examined the effect of dietary supplementation of Ficus carica leaf powder and vitamin C on the antioxidative status of the meat and brain of two hybrids of broiler chicken. Two hundred- and forty-day-old chicks, each of Arbor Acres (AA) and Cobb 500 (C5) hybrids of broiler chicken were randomly assigned to diets. Diet 1 and 2 had no supplement; diets 3 and 4 supplemented with vitamin C (200mg/kg of basal), diet 5 and 6 received 1g Ficus carica Leaf Powder (FCLP)/Kg of basal diet while diets 7 and 8 had 1g FCLP/kg of basal diet + 200mg of vitamin C. The interactive effect of hybrid, vitamin C and FCLP significantly influenced the dressing percentage, gizzard, pancreas, heart and proventriculus. Brain catalase and glutathione were significantly influenced by the hybrid, vitamin C and FCLP inclusion. Dietary supplementation of FCLP and vitamin C significantly lower the lipid peroxidation of the meat. There was significant interactive effect of the factors on lipid peroxidation while meat catalase and glutathione were significantly increased by the inclusion of FCLP. This study revealed that the dietary inclusions of broilers’ diets with 1g FCLP and 200mg vitamin C/kg of feed could influence carcass and organs weights as well as improve meat quality and brain antioxidant status of broiler chicken, with the C5 hybrid showing better antioxidative status. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: This study investigated the response of intake, ruminal fermentation, digestibility, N utilisation and growth performance in goats fed concentrate partially substituted with Parkia biglobosa foliage (PBF). Fifteen goats were randomly assigned to a control concentrate diet (PBF0) which was partially substituted with 25% PBF (PBF25) or 50% PBF (PBF50) on dry matter (DM) basis. Concentrate, TST and non-fibre carbohydrates intakes (linear and quadratic, P<0.001), digestible organic matter (DOM; linear, P=0.001) and urinary N (linear, P=0.039) decreased with increased PBF level. Condensed tannins intake, fibre fractions and cellulose digestibility, faecal N (linear and quadratic, P<0.05) and ruminal pH (quadratic, P=0.001) increased with increasing PBF level. Average daily gain, DM and crude protein (CP) digestibility, N intake and retention (quadratic, P<0.05), organic matter and hemicellulose digestibility, DM and nutrient intakes, energy concentration, ruminally-fermented DOM and microbial protein synthesis (linear and quadratic, P<0.05), and digestible CP and volatile fatty acids (linear, P<0.05) increased as PBF level increased. Feed:gain ratio and total N excretion (quadratic, P<0.05), and ruminal total N and ammonia N (linear and quadratic, P<0.05) decreased in PBF25. It is concluded that PBF is a cost-effective alternative supplement which could reduce the quantity of concentrate for goat feeding by 50%. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: The growth, haemato-biochemical indices, and antioxidant status of broiler chickens fed varying levels of Guanidino Acetic Acid (GAA) were evaluated over the course of an eight-week feeding trial. In low ME (2700Kcal/kg) basal diets, the Guanidino Acetic Acid was supplemented at 0, 0.6, and 1.2g/kg, designated as diets I, II, and III, respectively. The average final weight of chickens fed diet III was the highest at the end of the feeding trial. The best feed conversion ratio was observed in birds fed diet III. The packed cell volume and haemoglobin were highest in birds fed diet I. Significantly higher Cholesterol and Albumin were recorded in diets III and II, respectively. All the antioxidant parameters were significant except Catalase. The glutathione peroxidase increased with increased Guanidino acetic Acid supplementation while Catalase reduced with increased GAA supplementation. According to the findings of this study, Guanidino Acetic Acid improved the health of broilers while having no negative effects on their health. It is therefore concluded that supplementation of Guanidino Acetic Acid at 1.2g/kg with low metabolizable energy promoted superior growth effect of the chickens and also reduce cost of production. PubDate: Sat, 21 Oct 2023 00:00:00 GMT
Abstract: Enterococcus faecium (NCIMB 11181) was evaluated for survivability, safety, and capacity as probiotics utilization. Gram-positive, catalase-negative test, antibiotics susceptibility, hemolysis activity, pH and bile salts resistance were screened using selective microbiological media. The strain was phenotypically assessed for its principal probiotic properties. The capacity of fermentation based on biochemical tests was evaluated by API 20STREP and interpretation by apiwebTM Biomerieux (France) software (99.2% very good identification). After incubation at 37°C for 22-24 h in aerobic conditions, E. faecium involves 13.96 Log10 with an optical density (OD 600 nm) from 0.2 in the first 2 hours of growth to 0.9 value. The safety assessment on TSA agar showed that the strain revealed α-hemolysis. Resistance to 16 clinically relevant antibiotics, presented for our strain a range from intermediate (81.25%) to susceptible (6.25%), followed by resistance to erythromycin and colistin sulfate. The strain exhibited tolerance to acidic conditions and simulated gastric environment. Therefore, E. faecium NCIMB 11181 can be considered a safe strain without creating a risk to animal health status. It may be used as a probiotic preparation product for application in animal nutrition. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: The experiment studied the effects of different levels of Rumex patientia spp. leaf powder inclusion in laying hens’ diets on their physical parameters and yolk color intensity. The study was conducted on 168 Hy-line laying hens (30 weeks aged), assigned in 3 treatment groups (C, E1 and E2) and housed in an experimental hall equipped with Big Dutchman cages, placed on 3 tiers with digestibility cages (3 treatments × 28 replicates × 2 birds per cage). Feed and water were administrated ad-libitum. During the entire 5 weeks experimental period (one week of accommodation), performance parameters were recorded: average daily feed intake(g/day/hen), feed conversion rate (g feed/g egg), laying rate intensity (%) and average egg weight (g). Eggs yolk color is generated by carotenoids as a nutritional response, which depends on their amount, type, and ratio. One of the most important aspects in how consumers rate the quality of food is color. The experimental results demonstrated that an inclusion rate of 1.5% and 3% Rumex patientia species leaves powder improved the yolk color intensity without negative effects on production performances and egg quality parameters. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: Food occupies a principal role in human life, having a major impact on health status and representing diverse characteristics that ensure valuable nutrient intake. Ensuring human nutrition has become one of the essential problems facing mankind, and quantity, quality and proper use of food have a major effect on the human body. Many raw materials and food products spoil easily, considerably shortening their shelf life. Extending the shelf-life of food is required to remove seasonal consumption, enlarge availability to the consumer and decrease losses in perishable food products, resorting to some procedures to preserve their properties. If natural antioxidants are used as alternatives for synthetic antioxidants, customer claims for food without damaging residual substances can be satisfied, and the poultry industry would benefit considerably. The principal objective of this review was to describe and under light the current situation regarding the role and importance of the use of antioxidant compounds obtained from natural sources in order to improve the nutritional qualities of eggs following their inclusion in the feed of laying hens. Data from bibliographic sources are analyzed and presented. The characteristics of a number of 9 plants with antioxidant potential are described with an emphasis on their characteristics, utilization, and possible effects on egg quality, performance, and health. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: The study aimed to examine the meat quality of chickens from a dual-purpose cross and its parent lines. The trial was conducted in the experimental poultry farm of the Institute of Animal Science-Kostinbrod, Bulgaria. The dual-purpose chickens were obtained by crossing female layers (Line L) and dual-purpose cocks (BB, based on Bresse Gauloise). At the age of 9 weeks the male chickens from the parent lines and the dual-purpose cross were slaughtered. Six chickens of each genotype were subjected to breast and thigh meat quality analysis. The crossbred chickens tended to have lower pH in breast but the values of this parameter were higher in their thighs. The BB line showed the poorest water-holding capacity (WHC) in both breast and thigh meat cuts. This line and the crossbreed showed lighter meat compared to the L line. Except the ash content of the breast and thigh meat that was higher in the BB line, the proximate composition did not differ between the examined lines and their cross. The L and dual-purpose chickens tended to have higher intramuscular fat (IMF) but lower protein in thigh meat than the BB line. The correlation analysis revealed clear relationships between the meat quality traits and the proximate composition, however they differed for the breast and thigh meat. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: The paper investigated the phytochemical content and antioxidant activity of poplar buds (Populus nigra L.), turmeric (Curcuma longa) and ginger (Zingiber officinale) extracts. Proximate composition, total polyphenols, ß-carotene, vitamin E, minerals and antioxidant capacity were tested for all selected plants. The proximate analysis of selected plants showed that poplar buds had a higher content of crude protein, fat and fiber than turmeric and ginger. The extract of poplar buds had a significantly higher content of total polyphenols (87.20 mg of gallic acid/g) and vitamin E (210.53 μg/g) than in the extract of turmeric and ginger. The polyphenol content was found to be in the order: poplar buds>turmeric> ginger. The beta carotene content was significantly higher in poplar buds (2.99 μg/g) than in turmeric (1.67 μg/g). Regarding mineral content, turmeric showed the highest content in iron and copper; ginger showed the highest content in manganese and poplar buds had the highest content in zinc. Moreover, poplar buds possessed the highest antioxidant capacity both on vitamin C and E calibration curve, evidencing the rich content in such antioxidants. Therefore, based on the valuable nutritional composition confirmed through this study, poplar buds, turmeric and ginger can be recommended as phytogenic feed additives for poultry nutrition. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: It was studied the main indicators of the functionality of reproductive system and their interrelation with the productivity indices of imported cows populations Holstein breed, belonging to 3 ecological types: the Dutch, the French and the German and our indigenous Moldovian type Black-Motley breed. The service period, was the shortest in cows of Moldovian type of Black-Motley breed with 38.1 days (P˂0.001), 30.2 days (P˂0.001) and by 14.5 days (P˂0.01.), compared to the Holstein German, Dutch and French ecological types, respectively. The difference of the duration of calvings interval between indigenous population and French ecological type was 18.2 days (p<0.01), compared to the German 46 days (P<0.001) and to the Dutch 36,5 days (P<0.001). There has been a difference of 9,9% in birth coefficient, between Moldovian type and Holstein German ecological type (p˂0.05). The best result of the coefficient of reproductive capacity has the indigenous type, opposite of German ecological type, which has the lowest performance (P˂0.001). Between the service period and the productivity indicators of the milk, regardless of the origin of the animal populations, were predominantly established, authentic positive correlative interrelationships, from weak to strong and statistically negative between productivity indices and the coefficient of the reproductive capacity of cows. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: The study aimed to assess the effect of dietary inulin (1% dietary inclusion) supplements on broiler chickens’ meat antioxidant compounds. The experiment was carried out under heat-stress conditions, on 60 Ross 308 broiler chickens. The study took place in an experimental hall with digestibility pens and controlled humidity, ventilation and light regime. Each treatment (2 groups, C and E) was replicated 6 times with 5 chicks per replicate pen with ad libitum feeding and free water access. At 42 days old, 6 birds from each group were slaughtered and breast and thigh meat samples were collected. The presence of inulin in broiler chickens’ diets had a beneficial effect by increasing the iron and zinc concentrations in thigh samples. Regarding liposoluble antioxidants, the presence of inulin in diets from tissue samples had an antagonistic effect: increased concentrations of xanthophylls were registered in thigh meat and an adverse effect was noticed in breast meat while an inverse effect was noticed for vitamin E. At the thigh meat level, the antioxidant capacity was significantly improved under inulin influence. In conclusion, the nutritional quality of broilers meat (thigh meat) was improved, for the group fed with inulin-supplemented diets. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: The post-weaning period is very stressful for piglets, leading to a transitory inflammation, alterations in the intestinal barrier, oxidative stress and a higher susceptibility to intestinal diseases with economic losses. The finding of new nutritional alternatives with anti-inflammatory, anti-bacterial, anti-oxidant properties is a challenge in post-weaning piglet’s nutrition. Of these strategies, those based on agro-industry wastes rich in bioactive compounds are promising, the increasing number of studies focusing on the use of these wastes as successful modulators of post-weaning – associated disturbances in piglets. This review describes the role of grape by-products in the modulation of inflammation, reinforcement of the intestinal barrier and their role as antioxidant factors. Also, their in-depth intracellular mechanisms of action related to their anti-inflammatory, antioxidant properties are described. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: A six-week study was conducted to evaluate the effects of partial replacement of maize with Orange Fleshed Sweet Potato (OFSP) root meal on the haematological parameters and serum biochemistry of broiler chickens at finisher phase. One hundred and eight (108) day old broiler chicks were randomly allotted into 4 dietary treatments (T1, T2, T3 and T4) with 3 replicates each and 9 birds per replicate. The test ingredient, OFSP root meal was included in broiler finisher diets as replacement for maize at 0%, 6%, 12% and 18% in the dietary treatments respectively. The study revealed no effect on all the haematological parameters. The result of the serum biochemistry also showed no significant differences (P>0.05) on all the parameters measured with the exception of triglycerides, globulin and blood urea nitrogen with significant decrease in the triglycerides (mg/dl) and globulin (g/dl) content of T2(24.80 and 0.37), T3(47.01 and 1.07) and T4(48.50 and 1.41) respectively as compared with the control group (61.54 and 1.95 respectively). Therefore, inclusion of OFSP root meal up to 18% did not negatively affect the haematological parameters of the birds and also reduced the risk of metabolic diseases in the birds while improving the cardiovascular health of the birds. PubDate: Sat, 27 May 2023 00:00:00 GMT
Abstract: This study was conducted to test the effect of two levels of rosehip meal used together with flaxseed meal on weekly production performances and blood parameters of laying hens. For that, we conducted a trial for 5 weeks on 120 laying hens (Tetra SL hybrid), aged 29 weeks. All three diets had 17.50% crude protein and 2780Kcal/kg metabolizable energy, but compared to the control diet (Con) the experimental diets contained 1.5% rosehip meal and 7% flaxseed meal (Diet1) or, 3% rosehip meal and 7% flaxseed meal (Diet2). The results revealed that only feed intake was significantly (P=0.0001) affected, being lower in experimental groups. Among the haematological parameters, the heterophile were significantly higher (P=0.0377) in both Diet1 and Diet2 groups versus the Con group. From the biochemical parameters analysed, in the lipid profile, glucose and triglycerides were significantly lower in the Diet2 group compared with both the Diet1 and Con groups. Total bilirubin was significantly higher (P=0.0020) in the Diet1 group compared to the Con and Diet2 groups, while the lactate dehydrogenase was lower (P=0.0414) in the samples of the Diet2 group. Overall, the studied ingredients had a slight influence on production performances and improved some blood parameters, especially the lipid profile of the Diet2 group. PubDate: Sat, 27 May 2023 00:00:00 GMT
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