Authors:Triana Setyawardani; Juni Sumarmono, Heni Risqiati, Setya Agus Santosa Abstract: The study was aimed to investigate the antimicrobial activity of bacterial isolates L.plantarum 3CT7 and 20CT8 from goat colostrum. The antimicrobial activity of cell-free supernatant was tested using a well-diffusion method on several indicators: temperature, time of storage, and pH. Antimicrobial activity was recorded in both isolates at pH 2.0; 4.0; 6.0 and 8.0, temperature at 0, 50 and 100 oC, and in cold storage for 0, 15, 30, 45 and 60 days. L.plantarum 7CT3 and L.plantarum 20CT8 have a bigger zone of inhibition than that of Pseudomonas spp. as compared to other bacteria. Testing the cell-free activity was aimed to investigate the metabolite inhibition by L.plantarum. The isolates were capable of inhibiting all pathogenic bacteria in the experiment (S. thypimurium, E. coli, and S. aureus) as evidenced from the similar zone of inhibition from 15.83 to 16.06 mm. Isolates (L. plantarum 7CT3 dan 20CT8) exhibit inhibitory properties against S.thypimurium, S. aureus, Pseudomonas spp.. and PubDate: Wed, 25 Mar 2020 14:27:28 +000
Authors:Krismiwati Muatip; Hermin Purwaningsih, Agus Priyono, Muhammad Nuskhi, Lucie Setiana, Aditya Permana Putra Abstract: The study was conducted in the final waste disposal (TPA) Jatibarang, Kedungpane, Mijen, Semarang. A survey was conducted, targeting the beef cattle farmers who pastured their cattle in the TPA Jatibarang, Semarang. The observed variables were age (X1), education background (X2), length of stay (X3), total cattle owned (X4), and the farmer’s norm compliance (Y1). Purposive sampling was used to determine the sample of regions. Total respondents were determined by simple random sampling, selecting 70 out of 350 farmers (20%). The result of the analysis showed that 78.57% farmers were at their productive age (20-55 years old), 64.30% had elementary education background, 74.29% had been farming for over 14 years, and 62.86% had fewer than seven cattle. The farmers also showed a sound moral compliance. The result of spearman rank showed a non-significant correlation between age and length of farming experience with norm compliance. Age was negatively correlated with norm compliance, but education background and total cattle owned were significantly correlated with norm compliance, showing a correlation value of 0,248* and 0,610*, respectively. PubDate: Wed, 25 Mar 2020 14:27:28 +000