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  Subjects -> NUTRITION AND DIETETICS (Total: 201 journals)
Showing 1 - 64 of 64 Journals sorted by number of followers
American Journal of Clinical Nutrition     Hybrid Journal   (Followers: 176)
British Journal Of Nutrition     Hybrid Journal   (Followers: 96)
Clinical Nutrition     Hybrid Journal   (Followers: 94)
International Journal of Obesity     Hybrid Journal   (Followers: 93)
International Journal of Sport Nutrition & Exercise Metabolism     Hybrid Journal   (Followers: 88)
European Journal of Clinical Nutrition     Hybrid Journal   (Followers: 75)
Advances in Food and Nutrition Research     Full-text available via subscription   (Followers: 62)
Journal of the Academy of Nutrition and Dietetics     Full-text available via subscription   (Followers: 61)
Food Science & Nutrition     Open Access   (Followers: 59)
Obesity     Hybrid Journal   (Followers: 58)
Advances in Nutrition     Hybrid Journal   (Followers: 55)
Annals of Nutrition and Metabolism     Full-text available via subscription   (Followers: 52)
Journal of Pediatric Gastroenterology and Nutrition (JPGN)     Hybrid Journal   (Followers: 52)
Journal of Human Nutrition and Dietetics     Hybrid Journal   (Followers: 52)
Diabetes, Metabolic Syndrome and Obesity     Open Access   (Followers: 48)
American Journal of Food and Nutrition     Open Access   (Followers: 48)
Nutrition in Clinical Practice     Hybrid Journal   (Followers: 45)
Journal of Nutrition     Hybrid Journal   (Followers: 42)
Annual Review of Nutrition     Full-text available via subscription   (Followers: 40)
Nutrition Reviews     Hybrid Journal   (Followers: 38)
European Journal of Nutrition     Hybrid Journal   (Followers: 36)
Food & Nutrition Research     Open Access   (Followers: 35)
Journal of Parenteral and Enteral Nutrition     Hybrid Journal   (Followers: 35)
International Journal of Behavioral Nutrition and Physical Activity     Open Access   (Followers: 31)
Nutrition & Dietetics     Hybrid Journal   (Followers: 31)
Public Health Nutrition     Hybrid Journal   (Followers: 30)
Journal of Nutrition, Health and Aging     Hybrid Journal   (Followers: 30)
Journal of Renal Nutrition     Hybrid Journal   (Followers: 28)
Current Opinion in Clinical Nutrition & Metabolic Care     Hybrid Journal   (Followers: 26)
Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity     Hybrid Journal   (Followers: 25)
Appetite     Hybrid Journal   (Followers: 25)
Obesity Reviews     Hybrid Journal   (Followers: 25)
Current Nutrition & Food Science     Hybrid Journal   (Followers: 25)
Journal of Obesity     Open Access   (Followers: 24)
Childhood Obesity     Hybrid Journal   (Followers: 24)
International Journal of Nutrition and Metabolism     Open Access   (Followers: 23)
International Journal of Eating Disorders     Hybrid Journal   (Followers: 23)
Nutrition Research     Hybrid Journal   (Followers: 23)
Clinical Nutrition ESPEN     Hybrid Journal   (Followers: 23)
Advances in Eating Disorders : Theory, Research and Practice     Hybrid Journal   (Followers: 22)
Nutrition     Hybrid Journal   (Followers: 22)
Comparative Exercise Physiology     Hybrid Journal   (Followers: 21)
Nutrition & Diabetes     Open Access   (Followers: 20)
International Journal of Food Safety, Nutrition and Public Health     Hybrid Journal   (Followers: 20)
Journal of Nutrition Education and Behavior     Hybrid Journal   (Followers: 19)
Topics in Clinical Nutrition     Hybrid Journal   (Followers: 19)
Clinical Obesity     Hybrid Journal   (Followers: 18)
American Journal of Botany     Full-text available via subscription   (Followers: 18)
Canadian Journal of Dietetic Practice and Research     Full-text available via subscription   (Followers: 17)
Nutrition & Metabolism     Open Access   (Followers: 17)
African Journal of Food, Agriculture, Nutrition and Development     Open Access   (Followers: 17)
Journal of Nutrition and Metabolism     Open Access   (Followers: 16)
Journal of Advanced Nutrition and Human Metabolism     Open Access   (Followers: 16)
Nutrition and Dietary Supplements     Open Access   (Followers: 15)
Journal of Eating Disorders     Open Access   (Followers: 15)
Journal of Nutrition in Gerontology and Geriatrics     Hybrid Journal   (Followers: 15)
Maternal & Child Nutrition     Hybrid Journal   (Followers: 14)
Nutrition Today     Hybrid Journal   (Followers: 14)
Nutrition Research Reviews     Hybrid Journal   (Followers: 13)
Nutrition, Metabolism and Cardiovascular Diseases     Hybrid Journal   (Followers: 13)
Nutrition and Cancer     Hybrid Journal   (Followers: 13)
Food, Culture and Society: An International Journal of Multidisciplinary Research     Full-text available via subscription   (Followers: 13)
BMC Nutrition     Open Access   (Followers: 13)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Nutrients     Open Access   (Followers: 13)
Journal of Health, Population and Nutrition     Open Access   (Followers: 13)
Clinical Nutrition Insight     Full-text available via subscription   (Followers: 13)
Nutrition Journal     Open Access   (Followers: 12)
BMJ Nutrition, Prevention & Health     Open Access   (Followers: 12)
Asian Journal of Clinical Nutrition     Open Access   (Followers: 12)
Food and Foodways: Explorations in the History and Culture of     Hybrid Journal   (Followers: 12)
Advances in Digestive Medicine     Open Access   (Followers: 12)
International Journal of Food Sciences and Nutrition     Hybrid Journal   (Followers: 11)
Nutrition Bulletin     Hybrid Journal   (Followers: 11)
Frontiers in Nutrition     Open Access   (Followers: 11)
Ecology of Food and Nutrition     Hybrid Journal   (Followers: 10)
Asia Pacific Journal of Clinical Nutrition     Full-text available via subscription   (Followers: 10)
International Journal for Vitamin and Nutrition Research     Hybrid Journal   (Followers: 10)
Journal of Food and Nutrition Research     Open Access   (Followers: 10)
Journal of Dietary Supplements     Hybrid Journal   (Followers: 10)
American Journal of Food Technology     Open Access   (Followers: 9)
Pediatric Obesity     Hybrid Journal   (Followers: 9)
Nutritional Neuroscience : An International Journal on Nutrition, Diet and Nervous System     Hybrid Journal   (Followers: 9)
Obesity Facts     Open Access   (Followers: 9)
Nutrition & Food Science     Hybrid Journal   (Followers: 9)
Proceedings of the Nutrition Society     Hybrid Journal   (Followers: 8)
Current Nutrition Reports     Hybrid Journal   (Followers: 8)
Journal of Nutritional Biochemistry     Hybrid Journal   (Followers: 8)
Amino Acids     Hybrid Journal   (Followers: 8)
Nutrition and Health     Hybrid Journal   (Followers: 8)
Journal of Hunger & Environmental Nutrition     Hybrid Journal   (Followers: 7)
Journal of the American College of Nutrition     Hybrid Journal   (Followers: 7)
Current Developments in Nutrition     Open Access   (Followers: 6)
Journal of Food Chemistry and Nutrition     Open Access   (Followers: 6)
International Journal of Food Science and Nutrition Engineering     Open Access   (Followers: 6)
Molecular Nutrition & Food Research     Hybrid Journal   (Followers: 6)
International Journal of Child Health and Nutrition     Hybrid Journal   (Followers: 6)
Current Research in Nutrition and Food Science     Open Access   (Followers: 6)
Food and Nutrition Bulletin     Hybrid Journal   (Followers: 6)
Nutrition - Science en évolution     Full-text available via subscription   (Followers: 5)
Nutrition Bytes     Open Access   (Followers: 5)
Food Digestion     Hybrid Journal   (Followers: 5)
South African Journal of Clinical Nutrition     Open Access   (Followers: 5)
Bangladesh Journal of Nutrition     Open Access   (Followers: 5)
Plant Foods for Human Nutrition     Hybrid Journal   (Followers: 5)
Genes & Nutrition     Open Access   (Followers: 5)
Journal of Pharmacy and Nutrition Sciences     Open Access   (Followers: 5)
Universal Journal of Food and Nutrition Science     Open Access   (Followers: 4)
Nutrition and Metabolic Insights     Open Access   (Followers: 4)
Metabolism and Nutrition in Oncology     Open Access   (Followers: 4)
Journal of Medical Nutrition and Nutraceuticals     Open Access   (Followers: 4)
International Journal of Nutrition, Pharmacology, Neurological Diseases     Open Access   (Followers: 4)
World Food Policy     Hybrid Journal   (Followers: 3)
Journal of Agriculture, Food Systems, and Community Development     Open Access   (Followers: 3)
Frontiers in Sustainable Food Systems     Open Access   (Followers: 3)
Aktuelle Ernährungsmedizin     Hybrid Journal   (Followers: 3)
Nutrición Hospitalaria     Open Access   (Followers: 3)
PharmaNutrition     Hybrid Journal   (Followers: 3)
Revista Española de Nutrición Humana y Dietética     Open Access   (Followers: 3)
Ernährung & Medizin     Hybrid Journal   (Followers: 3)
Perspectivas en Nutrición Humana     Open Access   (Followers: 2)
Pakistan Journal of Nutrition     Open Access   (Followers: 2)
Oil Crop Science     Open Access   (Followers: 2)
Acta Portuguesa de Nutrição     Open Access   (Followers: 2)
Open Nutrition Journal     Open Access   (Followers: 2)
Journal of Spices and Aromatic Crops     Open Access   (Followers: 2)
Food Quality and Safety     Open Access   (Followers: 2)
Journal of Nutritional & Environmental Medicine     Full-text available via subscription   (Followers: 2)
Progress in Nutrition     Open Access   (Followers: 2)
Endocrinología, Diabetes y Nutrición (English Edition)     Hybrid Journal   (Followers: 2)
Revista Chilena de Nutricion     Open Access   (Followers: 2)
Journal of Renal Nutrition and Metabolism     Open Access   (Followers: 2)
International Journal of Gastroenterology, Hepatology, Transplant and Nutrition     Open Access   (Followers: 2)
Bioactive Carbohydrates and Dietary Fibre     Hybrid Journal   (Followers: 2)
Nigerian Food Journal     Full-text available via subscription   (Followers: 2)
Lifestyle Genomics     Open Access   (Followers: 2)
Journal of Nutritional Science     Open Access   (Followers: 2)
Food and Health     Open Access   (Followers: 1)
The Australian Coeliac     Full-text available via subscription   (Followers: 1)
Endocrinología, Diabetes y Nutrición     Full-text available via subscription   (Followers: 1)
Amerta Nutrition     Open Access   (Followers: 1)
Archive of Food and Nutritional Science     Open Access   (Followers: 1)
Open Obesity Journal     Open Access   (Followers: 1)
Food and Environmental Virology     Hybrid Journal   (Followers: 1)
Case Reports in Clinical Nutrition     Open Access   (Followers: 1)
European Journal of Nutrition & Food Safety     Open Access   (Followers: 1)
Journal of Food Science and Nutrition Therapy     Open Access   (Followers: 1)
Human Nutrition & Metabolism     Open Access   (Followers: 1)
Food Frontiers     Open Access   (Followers: 1)
Journal of Food & Nutritional Disorders     Hybrid Journal   (Followers: 1)
Plant Production Science     Open Access   (Followers: 1)
Egyptian Journal of Obesity, Diabetes and Endocrinology     Open Access   (Followers: 1)
Jurnal Penelitian Gizi dan Makanan     Open Access   (Followers: 1)
Journal of Ethnic Foods     Open Access   (Followers: 1)
Clinical Nutrition Experimental     Open Access   (Followers: 1)
RBONE - Revista Brasileira de Obesidade, Nutrição e Emagrecimento     Open Access   (Followers: 1)
Cahiers de Nutrition et de Diététique     Full-text available via subscription   (Followers: 1)
Indian Journal of Nutrition and Dietetics     Hybrid Journal   (Followers: 1)
Canadian Food Studies / La Revue canadienne des études sur l'alimentation     Open Access   (Followers: 1)
Revista Mexicana de Trastornos Alimentarios     Open Access   (Followers: 1)
RBNE - Revista Brasileira de Nutrição Esportiva     Open Access   (Followers: 1)
Jurnal Gizi dan Dietetik Indonesia : Indonesian Journal of Nutrition and Dietetics     Open Access   (Followers: 1)
Clinical Nutrition Open Science     Open Access  
Food Hydrocolloids for Health     Open Access  
npj Science of Food     Open Access  
Functional Foods in Health and Disease     Open Access  
Journal of Nutraceuticals and Herbal Medicine     Open Access  
Arab Journal of Nutrition and Exercise     Open Access  
Nutrire     Hybrid Journal  
UNICIÊNCIAS     Open Access  
Lifestyle Journal     Open Access  
Archivos Latinoamericanos de Nutrición     Open Access  
Revista Salud Pública y Nutrición     Open Access  
Open Food Science Journal     Open Access  
Segurança Alimentar e Nutricional     Open Access  
Indonesian Food and Nutrition Progress     Open Access  
Journal of Medicinal Herbs and Ethnomedicine     Open Access  
La Ciencia al Servicio de la Salud y Nutrición     Open Access  
Jurnal Riset Kesehatan     Open Access  
Jurnal Gizi Indonesia / The Indonesian Journal of Nutrition     Open Access  
Hacettepe University Faculty of Health Sciences Journal     Open Access  
Gazi Sağlık Bilimleri Dergisi     Open Access  
Media Gizi Indonesia     Open Access  
Jurnal Gizi Klinik Indonesia     Open Access  
NFS Journal     Open Access  
Journal of Nutrition & Intermediary Metabolism     Open Access  
Food and Waterborne Parasitology     Open Access  
Journal of Nutritional Ecology and Food Research     Full-text available via subscription  
Journal of Nutritional Disorders & Therapy     Open Access  
DEMETRA : Alimentação, Nutrição & Saúde     Open Access  
Nigerian Journal of Nutritional Sciences     Full-text available via subscription  
African Journal of Biomedical Research     Open Access  
Journal of the Australasian College of Nutritional and Environmental Medicine     Full-text available via subscription  
Médecine & Nutrition     Full-text available via subscription  
Journal of Sensory Studies     Hybrid Journal  
Journal of Muscle Foods     Hybrid Journal  

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Similar Journals
Journal Cover
Amino Acids
Journal Prestige (SJR): 1.135
Citation Impact (citeScore): 3
Number of Followers: 8  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1438-2199 - ISSN (Online) 0939-4451
Published by Springer-Verlag Homepage  [2467 journals]
  • Protein cysteine S-glycosylation: oxidative hydrolysis of protein
           S-glycosidic bonds in aqueous alkaline environments

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      Abstract: Abstract Some glycoproteins contain carbohydrates S-linked to cysteine (Cys) residues. However, relatively few S-glycosylated proteins have been detected, due to the lack of an effective research methodology. This work outlines a general concept for the detection of S-glycosylation sites in proteins. The approach was verified by exploratory experiments on a model mixture of β-S-glucosylated polypeptides obtained by the chemical transformation of lysozyme P00698. The model underwent two processes: (1) oxidative hydrolysis of S-glycosidic bonds under alkaline conditions to expose the thiol group of Cys residues; (2) thiol S-alkylation leading to thiol S-adduct formation at the former S-glycosylation sites. Oxidative hydrolysis was conducted in aqueous urea, dimethyl sulfoxide, or trifluoroethanol, with silver nitrate as the reaction promoter, in the presence of triethylamine and/or pyridine. The concurrent formation of stable protein silver thiolates, gluconic acid, and silver nanoclusters was observed. The essential de-metalation of protein silver thiolates using dithiothreitol preceded the S-labeling of Cys residues with 4-vinyl pyridine or a fluorescent reagent. The S-labeled model was sequenced by tandem mass spectrometry to obtain data on the modifications and their distribution over the protein chains. This enabled the efficiency of both S-glycosidic bonds hydrolysis and S-glycosylation site labeling to be evaluated. Suggestions are also given for testing this novel strategy on real proteomic samples.
      PubDate: 2022-12-02
       
  • Methylarginine efflux in nutrient-deprived yeast mitigates disruption of
           nitric oxide synthesis

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      Abstract: Abstract Protein arginine N-methyltransferases (PRMTs) have emerged as important actors in the eukaryotic stress response with implications in human disease, aging, and cell signaling. Intracellular free methylarginines contribute to cellular stress through their interaction with nitric oxide synthase (NOS). The arginine-dependent production of nitric oxide (NO), which is strongly inhibited by methylarginines, serves as a protective small molecule against oxidative stress in eukaryotic cells. NO signaling is highly conserved between higher and lower eukaryotes, although a canonical NOS homologue has yet to be identified in yeast. Since stress signaling pathways are well conserved among eukaryotes, yeast is an ideal model organism to study the implications of PRMTs and methylarginines during stress. We sought to explore the roles and fates of methylarginines in Saccharomyces cerevisiae. We starved methyltransferase-, autophagy-, and permease-related yeast knockouts by incubating them in water and monitored methylarginine production. We found that under starvation, methylarginines are expelled from yeast cells. We found that autophagy-deficient cells have an impaired ability to efflux methylarginines, which suggests that methylarginine-containing proteins are degraded via autophagy. For the first time, we determine that yeast take up methylarginines less readily than arginine, and we show that methylarginines impact yeast NO production. This study reveals that yeast circumvent a potential methylarginine toxicity by expelling them after autophagic degradation of arginine-modified proteins.
      PubDate: 2022-12-01
       
  • Dietary L-arginine supplementation increases the hepatic expression of
           AMP-activated protein kinase in rats

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      Abstract: Abstract The goal of this study was to elucidate the molecular mechanisms responsible for the anti-obesity effect of L-arginine supplementation in diet-induced obese rats. Male Sprague–Dawley rats were fed either a low-fat or high-fat diet for 15 weeks. Thereafter, lean or obese rats were pair-fed their same respective diets and received drinking water containing either 1.51% L-arginine-HCl or 2.55% L-alanine (isonitrogenous control) for 12 weeks. Gene and protein expression of key enzymes in the metabolism of energy substrates were determined using real-time polymerase-chain reaction and western blotting techniques. The mRNA levels of hepatic fatty acid synthase and stearoyl-CoA desaturase were reduced (P < 0.05) but those of hepatic AMP-activated protein kinase-α (AMPKα), peroxisome proliferator activator receptor γ coactivator-1α, and carnitine palmitoyltransferase I (CPT-I), as well as skeletal muscle CPT-I were increased (P < 0.05) by L-arginine treatment. The protein expression and activity of hepatic AMPKα markedly increased (P < 0.05) but the activity of hepatic acetyl-CoA carboxylase (ACC) decreased (P < 0.05) in response to dietary L-arginine supplementation. Collectively, our results indicate that liver is the major target for the action of dietary L-arginine supplementation on reducing white-fat mass in diet-induced obese rats by inhibiting fatty acid synthesis and increasing fatty acid oxidation via the AMPK-ACC signaling pathway. Additionally, increased CPT-I expression in skeletal muscle may also contribute to the enhanced oxidation of long-chain fatty acids in L-arginine-supplemented rats.
      PubDate: 2022-12-01
       
  • Taurine protects R28 cells from hypoxia/re-oxygenation-induced damage via
           regulation of mitochondrial energy metabolism

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      Abstract: Abstract Oxidative-induced damage and hypoxia/re-oxygenation (H/R) injury are common causes of irreversible visual impairment. The goals of this study were to explore the effects of taurine on R28 cells under the two damage models and the underlying mechanisms. Low doses of taurine supplementation promoted cell viability, mitochondrial membrane potential (MMP), SOD levels, ATP contents and attenuated cytotoxicity and intracellular ROS generation of the R28 cells under the two kinds of damage. The expression level of GTPBP3, a mitochondrial-tRNA (mt-tRNA) modification enzyme that catalyzes the taurine involved modification, was decreased under the two damage and taurine could reverse the reduction. After knocking down GTPBP3, the R28 cells become vulnerable to damage. The viability, cytotoxicity, MMP and intracellular ROS level of knockdown cells changed more obviously under the H/R injury than those of control cell. We also found that knockdown of GTPBP3 significantly decreased mitochondrial energy metabolism by measuring the oxidative respiration rate by the Seahorse XFe24 extracellular flux analyzer. The protection of low doses of taurine disappeared on knockdown R28 cells, indicating that GTPBP3 is crucial in the protection mechanisms of taurine. However, the impacts of the reduction of GTPBP3 level can be reversed by relatively high doses of taurine, implying the protection effects of taurine were dose-dependent, and there were more complicated mechanisms remain to be explored. This study explored a new mechanism of the neuroprotective effects of taurine, which depend on the GTPBP3-mediated taurine modification of mt-tRNAs and the promotion of mitochondrial energy metabolism.
      PubDate: 2022-12-01
       
  • Possible role of SIRT1 and SIRT3 in post-translational modifications in
           human breast milk during the neonatal period

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      Abstract: Abstract We measured free and proteinic concentrations of native and modified amino acids from post-translational modifications (PTMs) and correlated them with the activity of SIRT1 and SIRT3 in the pellet and aqueous phases of human breast milk samples of ten lactating women during the neonatal period. SIRT1 and SIRT3 correlated directly with citrullination, asymmetric dimethylation and glycation of L-arginine, hydroxylation and glycation of L-lysine. SIRT1 and SIRT3 correlated inversely with the hydroxylation of L-proline. SIRT1 and SITR3 tended to correlate inversely with oxidative stress measured as malondialdehyde. Our study suggests that SIRT1 and SIRT3 may modulate PTMs in human breast milk cells.
      PubDate: 2022-12-01
       
  • l-Arginine increases AMPK phosphorylation and the oxidation of energy
           substrates in hepatocytes, skeletal muscle cells, and adipocytes

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      Abstract: Abstract Previous work has shown that dietary l-arginine (Arg) supplementation reduced white fat mass in obese rats. The present study was conducted with cell models to define direct effects of Arg on energy-substrate oxidation in hepatocytes, skeletal muscle cells, and adipocytes. BNL CL.2 mouse hepatocytes, C2C12 mouse myotubes, and 3T3-L1 mouse adipocytes were treated with different extracellular concentrations of Arg (0, 15, 50, 100 and 400 µM) or 400 µM Arg + 0.5 mM NG-nitro-l-arginine methyl ester (L-NAME; an NOS inhibitor) for 48 h. Increasing Arg concentrations in culture medium dose-dependently enhanced (P < 0.05) the oxidation of glucose and oleic acid to CO2 in all three cell types, lactate release from C2C12 cells, and the incorporation of oleic acid into esterified lipids in BNL CL.2 and 3T3-L1 cells. Arg at 400 µM also stimulated (P < 0.05) the phosphorylation of AMP-activated protein kinase (AMPK) in all three cell types and increased (P < 0.05) NO production in C2C12 and BNL CL.2 cells. The inhibition of NOS by L-NAME moderately reduced (P < 0.05) glucose and oleic acid oxidation, lactate release, and the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) in BNL CL.2 cells, but had no effect (P > 0.05) on these variables in C2C12 or 3T3-L1 cells. Collectively, these results indicate that Arg increased AMPK activity and energy-substrate oxidation in BNL CL.2, C2C12, and 3T3-L1 cells through both NO-dependent and NO-independent mechanisms.
      PubDate: 2022-12-01
       
  • The post-translational modification of Fascin: impact on cell biology and
           its associations with inhibiting tumor metastasis

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      Abstract: Abstract The post-translational modifications (PTMs), which are crucial in the regulation of protein functions, have great potential as biomarkers of cancer status. Fascin (Fascin actin-bundling protein 1, FSCN1), a key protein in the formation of filopodia that is structurally based on actin filaments (F-actin), is significantly associated with tumor invasion and metastasis. Studies have revealed various regulatory mechanisms of human Fascin, including PTMs. Although a number of Fascin PTM sites have been identified, their exact functions and clinical significance are much less explored. This review explores studies on the functions of Fascin and briefly discusses the regulatory mechanisms of Fascin. Next, to review the role of Fascin PTMs in cell biology and their associations with metastatic disease, we discuss the advances in the characterization of Fascin PTMs, including phosphorylation, ubiquitination, sumoylation, and acetylation, and the main regulatory mechanisms are discussed. Fascin PTMs may be potential targets for therapy for metastatic disease.
      PubDate: 2022-12-01
       
  • Effects of taurine on vascular tone

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      Abstract: Abstract Taurine is widely distributed at high concentrations in mammalian tissues, and it plays an important role in a wide range of biological effects including modulation of cardiovascular functions. This review summarizes the role of taurine in vascular tone and blood pressure modulation based on experimental and human studies. It is well established that supplementation of taurine prevents development of hypertension in several animal models and p.o. taurine administration reduces blood pressure in hypertensive patients. Both central and peripheral actions of taurine may be involved in its hypotensive effects. In isolated animal arteries, taurine exerts vasodilation through endothelium-dependent and independent mechanisms. Several studies showed that taurine relaxed various animal arteries through opening potassium channels. We have recently shown that taurine relaxes human internal mammary and radial arteries by opening large conductance Ca2+-activated K+ channels. To date, the molecular mechanism(s) involved in the vascular effects of taurine are largely unknown and require further investigation. Clarifying the mechanisms in which taurine affects the vascular system may facilitate the development of therapeutic and/or diet-based strategies to reduce the burden of vascular diseases.
      PubDate: 2022-12-01
       
  • Divergent synthesis of biologically active l-threo-β-hydroxyaspartates
           from common trans-oxazolidine dicarboxylate

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      Abstract: Abstract A divergent synthetic strategy starting from a common trans-oxazolidine dicarboxylate intermediate has been successful to produce several non-proteinogenic l-threo-β-hydroxyaspartate derivatives efficiently with high stereoselectivity. Three bioactive α-amino-β-hydroxy acids, l-threo-β-hydroxyaspartic acid, l-threo-β-hydroxyasparagine, and l-threo-β-benzyloxyaspartic acid, were synthesized in good yields (58–83%) from the common chiral intermediate, and the chemoselective peptide bond formation at the α-amino group, β-hydroxy group, or α-carboxylic acid of the common intermediate was possible to afford the corresponding dipeptide, tripeptide, or didepsipeptide intermediate in 46~77% yields (in three-to-four steps) due to the orthogonal protective groups on the chiral intermediate.
      PubDate: 2022-12-01
       
  • Cul5 mediates taurine-stimulated mTOR mRNA expression and proliferation of
           mouse mammary epithelial cells

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      Abstract: Abstract Cullin5 (Cul5) protein can regulate multiple signaling pathways; however, it is still largely unknown the role and molecule mechanism of Cul5 in regulation of the mTOR signaling. In this study, we determined the effect of Cul5 on the proliferation of HC11 cells, a mouse mammary epithelial cell line, and explored the corresponding molecular mechanism. We found that Cul5 was highly expressed in mammary gland tissues in the lactation stage compared with that in puberty and involution. Using gene knockdown and activation methods, we showed that Cul5 promoted proliferation of HC11 cells, mRNA expression and protein phosphorylation of mTOR. Taurine (Tau) affected Cul5 mRNA and protein levels in a dose-dependent manner. Cul5 localized to the nucleus and knockdown of Cul5 almost totally blocked the stimulation of Tau on mTOR mRNA expression and protein phosphorylation. PI3K inhibition almost totally abolished the stimulation of Tau on Cul5 expression. In summary, our data uncover that Cul5 is a positive regulator of proliferation of HC11 cells, and mediates the stimulation of Tau on mRNA expression and subsequent protein phosphorylation of mTOR. Our data lay a new theoretical foundation for regulating mammary cell proliferation and promoting milk yield.
      PubDate: 2022-11-30
       
  • l-Arginine is a feasible supplement to heal chronic anal fissure via
           reducing internal anal sphincter pressure: a randomized clinical trial
           study

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      Abstract: Abstract The hypertonicity of internal anal sphincter resting pressure is one of the main causes of chronic anal fissure. Therefore, the aim of this study was to assess the effect of oral administration of l-arginine on the improvement of the anal fissures by relaxing the internal anal sphincter. Seventy-six chronic anal fissure patients (aged 18–65 years) who were referred to Rasoul-e-Akram Hospital, Tehran, Iran from February 2019 to October 2020 participated in this randomized, double-blind, placebo-controlled trial. Participants were allocated into treatment (l-arginine) and placebo groups. They took a 1000 mg capsule three times a day for 1 month, and then we followed them at the end of the first and third months after the intervention. Clinical symptoms, anal sphincter resting pressure, and quality of life (QoL) were completed at baseline and the end of the study. The analysis of data showed a significant decrease in bleeding, fissure size, and pain for each group; however, in the L-arginine group was more than the control group at the end of the study (P values < 0.001). Following that, a significant increase in QoL was seen just in patients treated with l-arginine (P value = 0.006). In addition, the comparison of anal pressures at baseline and, between groups at the end of the study showed a significant reduction in sphincter pressure in patients treated with l-arginine (P value < 0.001, = 0.049; respectively). The oral administration of 3000 mg l-arginine can heal chronic anal fissures by reducing internal anal sphincter pressure with more negligible side effects. However, we recommend long-term study with more extended follow-up. Clinical trial registry: IRCT20190712044182N1 at Iranian clinical trials, date: 2019-08-27.
      PubDate: 2022-11-30
       
  • Intracerebroventricular injection taurine changes free amino acid
           concentrations in the brain and plasma in chicks

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      Abstract: Abstract Brain amino acid metabolism has been reported to regulate body temperature, feeding behavior and stress response. Central injection of taurine induced hypothermic and anorexigenic effects in chicks. However, it is still unknown how the amino acid metabolism is influenced by the central injection of taurine. Therefore, the objective of this study was to investigate the changes in brain and plasma free amino acids following central injection of taurine. Five-day-old male Julia layer chicks (n = 10) were subjected to intracerebroventricular (ICV) injection with saline or taurine (5 µmol/10 µL). Central taurine increased tryptophan concentrations in the diencephalon, and decreased tyrosine in the diencephalon, brainstem, cerebellum, telencephalon and plasma at 30 min post-injection. Taurine was increased in all the brain parts after ICV taurine. Although histidine and cystathionine concentrations were increased in the diencephalon and brainstem, several amino acids such as isoleucine, arginine, methionine, phenylalanine, glutamic acid, asparagine, proline, and alanine were reduced following central injection of taurine. All amino acid concentrations were decreased in the plasma after ICV taurine. In conclusion, central taurine quickly changes free amino acid concentrations in the brain and plasma, which may have a role in thermoregulation, food intake and stress response in chicks.
      PubDate: 2022-11-27
       
  • Chemical properties of inner and surficial regions of hydrogen-bonded
           clusters of biological molecules: ultraviolet photodissociation and water
           adsorption analyses in the gas phase

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      Abstract: The chemical and physical properties of cold, gas-phase hydrogen-bonded clusters of l-alanine (l-Ala), l-trialanine (l-Ala3), l-tetraalanine (l-Ala4), and tryptophan (Trp) enantiomers were investigated using tandem mass spectrometry with an electrospray ionization source and cold ion trap. From the ultraviolet (UV) photodissociation spectra at 265–290 nm, the electronic structures of homochiral H+(l-Trp)(l-Ala) at 8 K were found to be different from those of heterochiral H+(d-Trp)(l-Ala) and protonated Trp. The number of water molecules adsorbed on the surface of gas-phase H+(d-Trp)(l-Ala) was larger than that of H+(l-Trp)(l-Ala), indicating stronger intermolecular interactions of l-Ala with H+(l-Trp) than those with H+(d-Trp). The product ion spectrum obtained by 265 nm photoexcitation of H+(l-Trp)(l-Ala3)(H2O)n formed via gas-phase water adsorption on H+(l-Trp)(l-Ala3) showed that the evaporation of water molecules was the main photodissociation process. In the case of H+(l-Trp)(l-Ala4)(H2O)n, signals of H+(l-Ala4) (H2O)n formed via l-Trp evaporation were observed in the product ion spectra, and the cross-section for UV photoinduced l-Trp evaporation became larger as the number of adsorbed water molecules increased. This observation indicates that water molecules were selectively adsorbed on the H+(l-Ala4) side of H+(l-Trp)(l-Ala4) and weakened the intermolecular interactions between l-Trp and H+(l-Ala4) in the hydrogen-bonded cluster.
      PubDate: 2022-11-21
       
  • The ovine conceptus utilizes extracellular serine, glucose, and fructose
           to generate formate via the one carbon metabolism pathway

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      Abstract: Abstract Highly proliferative cells rely on one carbon (1C) metabolism for production of formate required for synthesis of purines and thymidine for nucleic acid synthesis. This study was to determine if extracellular serine and/or glucose and fructose contribute the production of formate in ovine conceptuses. Suffolk ewes (n = 8) were synchronized to estrus, bred to fertile rams, and conceptuses were collected on Day 17 of gestation. Conceptuses were either snap frozen in liquid nitrogen (n = 3) or placed in culture in medium (n = 5) containing either: 1) 4 mM D-glucose + 2 mM [U-13C]serine; 2) 6 mM glycine + 4 mM D-glucose + 2 mM [U-13C]serine; 3) 4 mM D-fructose + 2 mM [U-13C]serine; 4) 6 mM glycine + 4 mM D-fructose + 2 mM [U-13C]serine; 5) 4 mM D-glucose + 4 mM D-fructose + 2 mM [U-13C]serine; or 6) 6 mM glycine + 4 mM D-glucose + 4 mM D-fructose + 2 mM [U-13C]serine. After 2 h incubation, conceptuses in their respective culture medium were homogenized and the supernatant analyzed for 12C- and 13C-formate by gas chromatography and amino acids by high performance liquid chromatography. Ovine conceptuses produced both 13C- and 12C-formate, indicating that the [U-13C]serine, glucose, and fructose were utilized to generate formate, respectively. Greater amounts of 12C-formate than 13C-formate were produced, indicating that the ovine conceptus utilized more glucose and fructose than serine to produce formate. This study is the first to demonstrate that both 1C metabolism and serinogenesis are active metabolic pathways in ovine conceptuses during the peri-implantation period of pregnancy, and that hexose sugars are the preferred substrate for generating formate required for nucleotide synthesis for proliferating trophectoderm cells.
      PubDate: 2022-11-16
       
  • Comparative effects of dietary methionine and cysteine supplementation on
           redox status and intestinal integrity in immunologically challenged-weaned
           pigs

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      Abstract: Abstract Sulfur-containing amino acids such as methionine and cysteine play critical roles in immune system and redox status. A body of evidence shows that metabolic aspects of supplemented Met and Cys may differ in the body. Therefore, the study aimed to investigate the effects of dietary Met and Cys supplementation in immunologically challenged weaned pigs. Forty weaned piglets (6.5 ± 0.3 kg) were randomly allocated to five treatment groups. The treatment included: (1) sham-challenged control (SCC), (2) challenged control (CC), (3) MET (CC + 0.1% DL-Met), (4) CYS (CC + 0.1% L-Cys), and (5) MET + CYS (CC + 0.1% DL-Met + 0.1% L-Cys). On day 7, all pigs were intramuscularly injected with either Escherichia coli O55:B5 lipopolysaccharides (LPS) or phosphate-buffered saline. Blood, liver, and jejunum samples were analyzed for immune response and redox status. The CC group had lower (P < 0.05) villus surface area and higher (P < 0.05) flux of 4-kDa fluorescein isothiocyanate dextran (FD4) than the SCC group. A lower (P < 0.05) glutathione (GSH) concentration was observed in the jejunum of pigs in the CC group than those in the SCC group. Dietary Cys supplementation increased (P < 0.05) villus surface area, GSH levels, and reduced (P < 0.05) the flux of FD4 in the jejunum of LPS-challenged pigs. Dietary Met supplementation enhanced (P < 0.05) hepatic GSH content. Pigs challenged with LPS in the MET group had lower serum IL-8 concentration than those in the CC group. There was a Met × Cys interaction (P < 0.05) in serum IL-4 and IL-8 concentrations, and Trolox equivalent antioxidant capacity. Dietary L-Cys supplementation restored intestinal integrity and GSH levels that were damaged by lipopolysaccharides administration. Dietary DL-Met supplementation improved hepatic GSH and reduced systemic inflammatory response, but antagonistic interaction with dietary L-Cys supplementation was observed in the inflammatory response and redox status.
      PubDate: 2022-11-13
       
  • Purification of an antiviral protein from the seeds of quinoa (Chenopodium
           quinoa Willd.) and characterization of its antiviral properties

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      Abstract: Abstract Plant viral pathogens cause damaging diseases in many agriculture systems, and emerging viral infections are a serious threat for providing adequate food to a continuously growing population. Recent studies of biogenic substances have provided new opportunities for producing novel antiviral agents. The present work has been conducted to evaluate the antiviral activity of quinoa (Chenopodium quinoa Willd.) seeds crude extract. The antiviral activity was retained in different buffer solutions of various pH ranges (5.2–8.5) and remained after the diafiltration process. The putative virus inhibitor was sensitive to treatment with sodium dodecyl sulfate and trichloroacetic acid. An antiviral protein with ~ 25 kDa molecular weight was isolated from the seed quinoa extract using ammonium sulfate precipitation, anion and cation exchange chromatography. The purified protein (Quinoin-I) significantly inhibited TMV on tobacco leaves with an IC50 value at a 6.81 μg/ml concentration. Enzyme activity assay revealed the RNase activity of Quinoin-I, and this feature was retained in the presence of β-mercaptoethanol and ethylene diamine tetraacetic acid. This antiviral protein has been shown as a promising leading molecule for further development as a novel antiviral agent.
      PubDate: 2022-11-08
       
  • An active domain SA-2 derived from cystatin-SA, and its antifungal
           activity

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      Abstract: Abstract Infections induced by fungi, especially the drug-resistant fungi, are difficult clinical problems. Conventional antifungal treatment is effective but due to resistance, treatment failure, and treatment-related toxicity, there is a need for new antifungal drugs. In this study, SA-2 (YYRRLLRVLRRRW) was derived from Cystatin-SA, a saliva protein with a molecular weight of 14 kDa. Meanwhile, the structure–activity of SA-2 and its mutants was also studied. We detected the antimicrobial activity and cytotoxicity of SA-2 and found that SA-2 had a low cytotoxicity toward mammalian cells but a good inhibitory effect on Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), with MIC values of 16–64 μg/mL and 8–32 μg/mL, respectively. Interestingly, SA-2 effectively killed fluconazole-resistant C. neoformans and C. albicans within 12 h. This antifungal activity against fluconazole-resistant fungi was comparable to that of amphotericin B. In addition, the C. neoformans-infected mice model was established to evaluate the anti-infective activity of SA-2 in vivo. Results showed that SA-2 significantly reduced the counts of fungi in lung and brain tissues to protect fluconazole-resistant C. neoformans-infected mice from death without changing mice body weights. Moreover, the dramatically increased pro-inflammatory cytokines TNF-α, IL-6 and IL-1β induced by intranasal infection of C. neoformans could be obviously declined due to the treatment of SA-2, which may be attributed to the elimination of C. neoformans in time in the infected tissue. For the mode of actions underlying SA-2 against C. neoformans, we found that the cationic peptide SA-2 could adhere to the negatively charged fungal cell membrane to increase the surface potential of C. neoformans in a dose-dependent manner, and finally disrupted the integrity of fungal cell membrane, reflecting as a 60% positive rate of propidium iodide uptake of C. neoformans cells after SA-2 (4 × MIC) treatment. Our study indicated that SA-2 has the potential to develop as a new therapeutic agent against infection induced by drug-resistant fungi.
      PubDate: 2022-11-04
       
  • Imidazole-amino acids. Conformational switch under tautomer and pH change

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      Abstract: Abstract Replacement of the main chain peptide bond by imidazole ring seems to be a promising tool for the peptide-based drug design, due to the specific prototropic tautomeric as well as amphoteric properties. In this study, we present that both tautomer and pH change can cause a conformational switch of the studied residues of alanine (1–4) and dehydroalanine (5–8) with the C-terminal peptide group replaced by imidazole. The DFT methods are applied and an environment of increasing polarity is simulated. The conformational maps (Ramachandram diagrams) are presented and the stability of possible conformations is discussed. The neutral forms, tautomers τ (1) and π (2), adapt the conformations αRτ (φ, ψ = − 75°, − 114°) and C7eq (φ, ψ = − 75°, 66°), respectively. Their torsion angles ψ differ by about 180°, which results in a considerable impact on the peptide chain conformation. The cation form (3) adapts both these conformations, whereas the anion analogue (4) prefers the conformations C5 (φ, ψ = − 165°, − 178°) and β2 (φ, ψ ~ − 165°, − 3°). Dehydroamino acid analogues, the tautomers τ (5) and π (6) as well as the anion form (8), have a strong tendency toward the conformations β2 (φ, ψ = − 179°, 0°) and C5 (φ, ψ = − 180°, 180°). The preferences of the protonated imidazolium form (7) depend on the environment. The imidazole ring, acting as a donor or acceptor of the hydrogen bonds created within the studied residues, has a profound effect on the type of conformation.
      PubDate: 2022-11-02
       
  • A novel family of non-secreted tridecaptin lipopeptide produced by
           Paenibacillus elgii

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      Abstract: Abstract Bacteria from the genus Paenibacillus make a variety of antimicrobial compounds, including lipopeptides produced by a non-ribosomal synthesis mechanism (NRPS). In the present study, we show the genomic and phenotypical characterization of Paenibacillus elgii AC13 which makes three groups of small molecules: the antimicrobial pelgipeptins and two other families of peptides that have not been described in P. elgii. A family of lipopeptides with [M + H]+ 1664, 1678, 1702, and 1717 m/z was purified from the culture cell fraction. Partial characterization revealed that they are similar to tridecaptin from P. terrae. However, they present amino acid chain modifications in positions 3, 7, and 10. These new variants were named tridecaptin G1, G2, G3, and G4. Furthermore, a gene cluster was identified in P. elgii AC13 genome, revealing high similarity to the tridecaptin-NRPS gene cluster from P. terrae. Tridecaptin G1 and G2 showed in vitro antimicrobial activity against Escherichia coli, Klebsiella pneumonia (including a multidrug-resistant strain), Staphylococcus aureus, and Candida albicans. Tri G3 did not show antimicrobial activity against S. aureus and C. albicans at all tested concentrations. An intriguing feature of this family of lipopeptides is that it was only observed in the cell fraction of the P. elgii AC13 culture, which could be a result of the amino acid sequence modifications presented in these variants.
      PubDate: 2022-11-01
       
  • Branched-chain amino acids regulate intracellular protein turnover in
           porcine mammary epithelial cells

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      Abstract: Abstract Dietary supplementation with branched-chain amino acids (BCAAs) to lactating sows has been reported to enhance their milk production, but the underlying mechanisms remain largely unknown. This study was conducted with porcine mammary epithelial cells (PMECs) to test the hypothesis that individual BCAAs or their mixture stimulates protein synthesis and inhibit proteolysis in PMECs. Cells were cultured at 37 °C in customized Dulbecco’s modified Eagle medium containing 5 mmol/L D-glucose, 1 mmol/L L-phenylalanine, L-[ring-2,4-3H]phenylalanine, 0.1 (control), 0.25, 0.5, 1, or 2 mmol/L L-leucine, L-isoleucine or L-valine or an equimolar mixture of the three BCAAs. The culture medium also contained physiological concentrations of other amino acids found in the plasma of lactating sows. Proliferation, protein synthesis, proteolysis, β-casein production, the mechanistic target of rapamycin (mTOR) signaling, and the ubiquitin–proteasome pathway were determined for PMECs. Cell proliferation and abundances of phosphorylated mTOR, eukaryotic translation initiation factor 4E-binding protein 1, and ribosomal protein S6 kinase β-1 proteins increased (P < 0.05), but abundances of ubiquitinated protein and 20S proteasome decreased (P < 0.05) when extracellular concentrations of L-leucine, L-isoleucine, L-valine, or an equimolar mixture of BCAAs were increased from 0.1 to 2 mmol/L. Compared with the control, 0.25, 0.5, 1 or 2 mmol/L BCAAs enhanced (P < 0.01) protein (including β-casein) synthesis, while decreasing (P < 0.05) proteolysis in PMECs in a dose-dependent manner. Collectively, our results indicate that physiological concentrations of BCAAs regulate protein turnover in mammary epithelial cells to favor net protein synthesis through stimulating the mTOR signaling pathway and inhibiting the ubiquitin–proteasome pathway.
      PubDate: 2022-09-09
      DOI: 10.1007/s00726-022-03203-y
       
 
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