Subjects -> AGRICULTURE (Total: 963 journals)
    - AGRICULTURAL ECONOMICS (93 journals)
    - AGRICULTURE (662 journals)
    - CROP PRODUCTION AND SOIL (120 journals)
    - DAIRYING AND DAIRY PRODUCTS (30 journals)
    - POULTRY AND LIVESTOCK (58 journals)

AGRICULTURE (662 journals)            First | 1 2 3 4     

Showing 601 - 263 of 263 Journals sorted by number of followers
Sustainability and Climate Change     Full-text available via subscription   (Followers: 15)
International Journal of Food Science and Agriculture     Open Access   (Followers: 13)
Peer Community Journal     Open Access   (Followers: 12)
Potato Journal     Open Access   (Followers: 11)
Indian Journal of Extension Education     Open Access   (Followers: 11)
Journal of Sugarcane Research     Open Access   (Followers: 11)
Annals of Arid Zone     Open Access   (Followers: 10)
The Journal of Research, PJTSAU     Open Access   (Followers: 10)
Magazín Ruralidades y Territorialidades     Full-text available via subscription   (Followers: 9)
Journal of the Indian Society of Soil Science     Open Access   (Followers: 8)
Indian Journal of Horticulture     Open Access   (Followers: 7)
Future Foods     Open Access   (Followers: 7)
Journal of Cereal Research     Open Access   (Followers: 6)
Animal - Open Space     Open Access   (Followers: 6)
Indian Journal of Animal Nutrition     Open Access   (Followers: 5)
aBIOTECH : An International Journal on Plant Biotechnology and Agricultural Sciences     Hybrid Journal   (Followers: 5)
Journal of the Indian Society of Coastal Agricultural Research     Open Access   (Followers: 5)
Revista Investigaciones Agropecuarias     Open Access   (Followers: 5)
Sustainability Agri Food and Environmental Research     Open Access   (Followers: 4)
Journal of Rural and Community Development     Open Access   (Followers: 3)
Asia-Pacific Journal of Rural Development     Hybrid Journal   (Followers: 3)
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan / Journal of Agricultural Sciences and Veteriner)     Open Access   (Followers: 3)
Molecular Horticulture     Open Access   (Followers: 2)
Animal Diseases     Open Access   (Followers: 2)
Grassland Research     Open Access   (Followers: 2)
Animal Microbiome     Open Access   (Followers: 2)
Plant Phenomics     Open Access   (Followers: 2)
CABI Agriculture and Bioscience     Open Access   (Followers: 2)
Journal of Animal Science and Products     Open Access   (Followers: 2)
Horticultural Studies     Full-text available via subscription   (Followers: 1)
PRIMA : Journal of Community Empowering and Services     Open Access   (Followers: 1)
Acta Scientiarum Polonorum Technica Agraria     Open Access   (Followers: 1)
Journal of Applied Communications     Open Access   (Followers: 1)
VITIS : Journal of Grapevine Research     Open Access   (Followers: 1)
Proceedings of the Vertebrate Pest Conference     Open Access   (Followers: 1)
Measurement : Food     Open Access   (Followers: 1)
Agriscience     Open Access   (Followers: 1)
CSA News     Hybrid Journal   (Followers: 1)
Analytical Science Advances     Open Access   (Followers: 1)
Journal of Environmental and Agricultural Studies     Open Access   (Followers: 1)
International Journal of Agricultural and Life Sciences     Open Access   (Followers: 1)
Energy Nexus     Open Access  
International Journal on Food, Agriculture and Natural Resources : IJ-FANRES     Open Access  
Reproduction and Breeding     Open Access  
Archiva Zootehnica     Open Access  
Journal of Agriculture and Food Research     Open Access  
Phytopathology Research     Open Access  
Rekayasa     Open Access  
Turkish Journal of Agricultural Engineering Research     Open Access  
Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi     Open Access  
Viticulture Data Journal     Open Access  
Ethiopian Journal of Sciences and Sustainable Development     Open Access  
Nexo Agropecuario     Open Access  
Dissertationen aus dem Julius Kühn-Institut     Open Access  
Berichte aus dem Julius Kühn-Institut     Open Access  
Journal für Kulturpflanzen     Open Access  
Food and Ecological Systems Modelling Journal     Open Access  
Journal of Animal Science, Biology and Bioeconomy     Open Access  
Agrosains : Jurnal Penelitian Agronomi     Open Access  
Agrotechnology Research Journal     Open Access  
Dinamika Pertanian     Open Access  
International Journal of Agricultural Science and Food Technology     Open Access  

  First | 1 2 3 4     

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aBIOTECH : An International Journal on Plant Biotechnology and Agricultural Sciences
Number of Followers: 5  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Online) 2662-1738
Published by Springer-Verlag Homepage  [2467 journals]
  • G2-LIKE CAROTENOID REGULATOR (SlGCR) is a positive regulator of lutein
           biosynthesis in tomato

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      Abstract: Abstract Lutein is an oxygen-containing carotenoid synthesized in plant chloroplasts and chromoplasts. It plays an indispensable role in promoting plant growth and maintaining eye health in humans. The rate-limiting step of lutein biosynthesis is catalyzed by the lycopene ε-cyclase enzyme (LCYE). Although great progress has been made in the identification of transcription factors involved in the lutein biosynthetic pathway, many systematic molecular mechanisms remain to be elucidated. Here, using co-expression analysis, we identified a gene, G2-LIKE CAROTENOID REGULATOR (SlGCR), encoding a GARP G2-like transcription factor, as the potential regulator of SlLCYE in tomato. Silencing of SlGCR reduced the expression of carotenoid biosynthetic genes and the accumulation of carotenoids in tomato leaves. By contrast, overexpression of SlGCR in tomato fruit significantly increased the expression of relevant genes and enhanced the accumulation of carotenoids. SlGCR can directly bind to the SlLCYE promoter and activate its expression. In addition, we also discovered that expression of SlGCR was negatively regulated by the master regulator SlRIN, thereby inhibiting lutein synthesis during tomato fruit ripening. Taken together, we identified SlGCR as a novel regulator involved in tomato lutein biosynthesis, elucidated the regulatory mechanism, and provided a potential tool for tomato lutein metabolic engineering.
      PubDate: 2022-11-29
       
  • Transcription factor CsESE3 positively modulates both jasmonic acid and
           wax biosynthesis in citrus

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      Abstract: Abstract PLIP lipases can initiate jasmonic acid (JA) biosynthesis. However, little is known about the transcriptional regulation of this process. In this study, an ERF transcription factor (CsESE3) was found to be co-expressed with all necessary genes for JA biosynthesis and several key genes for wax biosynthesis in transcriptomes of ‘Newhall’ navel orange. CsESE3 shows partial sequence similarity to the well-known wax regulator SHINEs (SHNs), but lacks a complete MM protein domain. Ectopic overexpression of CsESE3 in tomato (OE) resulted in reduction of fruit surface brightness and dwarf phenotype compared to the wild type. The OE tomato lines also showed significant increases in the content of wax and JA and the expression of key genes related to their biosynthesis. Overexpression of CsESE3 in citrus callus and fruit enhanced the JA content and the expression of JA biosynthetic genes. Furthermore, CsESE3 could bind to and activate the promoters of two phospholipases from the PLIP gene family to initiate JA biosynthesis. Overall, this study indicated that CsESE3 could mediate JA biosynthesis by activating PLIP genes and positively modulate wax biosynthesis. The findings provide important insights into the coordinated control of two defense strategies of plants represented by wax and JA biosynthesis.
      PubDate: 2022-11-22
       
  • Solanaceae pangenomes are coming of graphical age to bring heritability
           back

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      Abstract: Abstract Two recent articles describe a pangenome of potato and a graph-based pangenome for tomato, respectively. The latter improves our understanding of the tomato genomics architecture even further and the use of this graph-based pangenome versus a single reference dramatically improves heritability in tomato.
      PubDate: 2022-11-14
       
  • The genomic and bulked segregant analysis of Curcuma alismatifolia
           revealed its diverse bract pigmentation

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      Abstract: Abstract Compared with most flowers where the showy part comprises specialized leaves (petals) directly subtending the reproductive structures, most Zingiberaceae species produce showy “flowers” through modifications of leaves (bracts) subtending the true flowers throughout an inflorescence. Curcuma alismatifolia, belonging to the Zingiberaceae family, a plant species originating from Southeast Asia, has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different cultivars. Here, we present the chromosome-scale genome assembly of C. alismatifolia “Chiang Mai Pink” and explore the underlying mechanisms of bract pigmentation. Comparative genomic analysis revealed C. alismatifolia contains a residual signal of whole-genome duplication. Duplicated genes, including pigment-related genes, exhibit functional and structural differentiation resulting in diverse bract colors among C. alismatifolia cultivars. In addition, we identified the key genes that produce different colored bracts in C. alismatifolia, such as F3′5'H, DFR, ANS and several transcription factors for anthocyanin synthesis, as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis, bulked segregant analysis using both DNA and RNA data, and population genomic analysis. This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C. alismatifolia and related species. It is also important to understand the variation in the evolution of the Zingiberaceae family.
      PubDate: 2022-10-06
       
  • LncPheDB: a genome-wide lncRNAs regulated phenotypes database in plants

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      Abstract: Abstract LncPheDB (https://www.lncphedb.com/) is a systematic resource of genome-wide long non-coding RNAs (lncRNAs)-phenotypes associations for multiple species. It was established to display the genome-wide lncRNA annotations, target genes prediction, variant-trait associations, gene-phenotype correlations, lncRNA-phenotype correlations, and the similar non-coding regions of the queried sequence in multiple species. LncPheDB sorted out a total of 203,391 lncRNA sequences, 2000 phenotypes, and 120,271 variants of nine species (Zea mays L., Gossypium barbadense L., Triticum aestivum L., Lycopersicon esculentum Mille, Oryza sativa L., Hordeum vulgare L., Sorghum bicolor L., Glycine max L., and Cucumis sativus L.). By exploring the relationship between lncRNAs and the genomic position of variants in genome-wide association analysis, a total of 68,862 lncRNAs were found to be related to the diversity of agronomic traits. More importantly, to facilitate the study of the functions of lncRNAs, we analyzed the possible target genes of lncRNAs, constructed a blast tool for performing similar fragmentation studies in all species, linked the pages of phenotypic studies related to lncRNAs that possess similar fragments and constructed their regulatory networks. In addition, LncPheDB also provides a user-friendly interface, a genome visualization platform, and multi-level and multi-modal convenient data search engine. We believe that LncPheDB plays a crucial role in mining lncRNA-related plant data.
      PubDate: 2022-10-05
       
  • Advances in plastid transformation for metabolic engineering in higher
           plants

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      Abstract: Abstract The plastid (chloroplast) genome of higher plants is an appealing target for metabolic engineering via genetic transformation. Although the bacterial-type plastid genome is small compared with the nuclear genome, it can accommodate large quantities of foreign genes that precisely integrate through homologous recombination. Engineering complex metabolic pathways in plants often requires simultaneous and concerted expression of multiple transgenes, the possibility of stacking several transgenes in synthetic operons makes the transplastomic approach amazing. The potential for extraordinarily high-level transgene expression, absence of epigenetic gene silencing and transgene containment due to the exclusion of plastids from pollen transmission in most angiosperm species further add to the attractiveness of plastid transformation technology. This minireview describes recent advances in expanding the toolboxes for plastid genome engineering, and highlights selected high-value metabolites produced using transplastomic plants, including artemisinin, astaxanthin and paclitaxel.
      PubDate: 2022-10-01
       
  • Fundamental and practical approaches for single-cell ATAC-seq analysis

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      Abstract: Abstract Assays for transposase-accessible chromatin through high-throughput sequencing (ATAC-seq) are effective tools in the study of genome-wide chromatin accessibility landscapes. With the rapid development of single-cell technology, open chromatin regions that play essential roles in epigenetic regulation have been measured at the single-cell level using single-cell ATAC-seq approaches. The application of scATAC-seq has become as popular as that of scRNA-seq. However, owing to the nature of scATAC-seq data, which are sparse and noisy, processing the data requires different methodologies and empirical experience. This review presents a practical guide for processing scATAC-seq data, from quality evaluation to downstream analysis, for various applications. In addition to the epigenomic profiling from scATAC-seq, we also discuss recent studies in which the function of non-coding variants has been investigated based on cell type-specific cis-regulatory elements and how to use the by-product genetic information obtained from scATAC-seq to infer single-cell copy number variants and trace cell lineage. We anticipate that this review will assist researchers in designing and implementing scATAC-seq assays to facilitate research in diverse fields.
      PubDate: 2022-09-27
       
  • The global integrative network: integration of signaling and metabolic
           pathways

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      Abstract: Abstract The crosstalk between signaling and metabolic pathways has been known to play key roles in human diseases and plant biological processes. The integration of signaling and metabolic pathways can provide an essential reference framework for crosstalk analysis. However, current databases use distinct structures to present signaling and metabolic pathways, which leads to the chaos in the integrated networks. Moreover, for the metabolic pathways, the metabolic enzymes and the reactions are disconnected by the current widely accepted layout of edges and nodes, which hinders the topological analysis of the integrated networks. Here, we propose a novel “meta-pathway” structure, which uses the uniformed structure to display the signaling and metabolic pathways, and resolves the difficulty in linking the metabolic enzymes to the reactions topologically. We compiled a comprehensive collection of global integrative networks (GINs) by merging the meta-pathways of 7077 species. We demonstrated the assembly of the signaling and metabolic pathways using the GINs of four species—human, mouse, Arabidopsis, and rice. Almost all of the nodes were assembled into one major network for each of the four species, which provided opportunities for robust crosstalk and topological analysis, and knowledge graph construction.
      PubDate: 2022-09-21
      DOI: 10.1007/s42994-022-00078-1
       
  • Construction of homozygous diploid potato through maternal haploid
           induction

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      Abstract: Abstract Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes, but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines. To resolve this impediment, we developed a doubled haploid (DH) technology, based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (StDMP) gene. Here, we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines. An exercisable protocol, involving hybridization, fluorescent marker screening, molecular and flow cytometric identification, and doubling with colchicine generates nearly 100% homozygous diploid potato lines. This dmp-triggered haploid induction (HI) system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.
      PubDate: 2022-09-15
      DOI: 10.1007/s42994-022-00080-7
       
  • Coculture engineering for efficient production of vanillyl alcohol in
           Escherichia coli

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      Abstract: Abstract Vanillyl alcohol is a precursor of vanillin, which is one of the most widely used flavor compounds. Currently, vanillyl alcohol biosynthesis still encounters the problem of low efficiency. In this study, coculture engineering was adopted to improve production efficiency of vanillyl alcohol in E. coli. First, two pathways were compared for biosynthesis of the immediate precursor 3, 4-dihydroxybenzyl alcohol in monocultures, and the 3-dehydroshikimate-derived pathway showed higher efficiency than the 4-hydroxybenzoate-derived pathway. To enhance the efficiency of the last methylation step, two strategies were used, and strengthening S-adenosylmethionine (SAM) regeneration showed positive effect while strengthening SAM biosynthesis showed negative effect. Then, the optimized pathway was assembled in a single cell. However, the biosynthetic efficiency was still low, and was not significantly improved by modular optimization of pathway genes. Thus, coculturing engineering strategy was adopted. At the optimal inoculation ratio, the titer reached 328.9 mg/L. Further, gene aroE was knocked out to reduce cell growth and improve 3,4-DHBA biosynthesis of the upstream strain. As a result, the titer was improved to 559.4 mg/L in shake flasks and to 3.89 g/L in fed-batch fermentation. These are the highest reported titers of vanillyl alcohol so far. This work provides an effective strategy for sustainable production of vanillyl alcohol.
      PubDate: 2022-09-05
      DOI: 10.1007/s42994-022-00079-0
       
  • Transcriptome-wide N6-methyladenosine (m6A) methylation in soybean under
           Meloidogyne incognita infection

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      Abstract: Abstract N6-methyladenosine (m6A) is a reversible epigenetic modification of mRNA and other RNAs that plays a significant role in regulating gene expression and biological processes. However, m6A abundance, dynamics, and transcriptional regulatory mechanisms remain unexplored in the context of soybean resistance to Meloidogyne incognita. In this study, we performed a comparative analysis of transcriptome-wide m6A and metabolome profiles of soybean root tissues with and without M. incognita infection. Global m6A hypermethylation was widely induced in response to M. incognita infection and was enriched around the 3′ end of coding sequences and in 3′ UTR regions. There were 2069 significantly modified m6A sites, 594 differentially expressed genes, and 103 differentially accumulated metabolites between infected and uninfected roots, including coumestrol, psoralidin, and 2-hydroxyethylphosphonate. Among 101 m6A-modified DEGs, 34 genes were hypomethylated and upregulated, and 39 genes were hypermethylated and downregulated, indicating a highly negative correlation between m6A methylation and gene transcript abundance. A number of these m6A-modified DEGs, including WRKY70, ERF60, POD47 and LRR receptor-like serine/threonine-protein kinases, were involved in plant defense responses. Our study provides new insights into the critical role of m6A modification in early soybean responses to M. incognita.
      PubDate: 2022-08-18
      DOI: 10.1007/s42994-022-00077-2
       
  • The application of CRISPR/Cas technologies to Brassica crops: current
           progress and future perspectives

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      Abstract: Abstract Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of Brassica crops.
      PubDate: 2022-07-02
      DOI: 10.1007/s42994-022-00076-3
       
  • Current overview on the genetic basis of key genes involved in soybean
           domestication

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      Abstract: Abstract Modern crops were created through the domestication and genetic introgression of wild relatives and adaptive differentiation in new environments. Identifying the domestication-related genes and unveiling their molecular diversity provide clues for understanding how the domesticated variants were selected by ancient people, elucidating how and where these crops were domesticated. Molecular genetics and genomics have explored some domestication-related genes in soybean (Glycine max). Here, we summarize recent studies about the quantitative trait locus (QTL) and genes involved in the domestication traits, introduce the functions of these genes, clarify which alleles of domesticated genes were selected during domestication. A deeper understanding of soybean domestication could help to break the bottleneck of modern breeding by highlighting unused genetic diversity not selected in the original domestication process, as well as highlighting promising new avenues for the identification and research of important agronomic traits among different crop species.
      PubDate: 2022-07-02
      DOI: 10.1007/s42994-022-00074-5
       
  • Disruption of three polyamine uptake transporter genes in rice by
           CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat

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      Abstract: Abstract Weeds are a major biotic constraint that can cause dramatic crop production losses. Herbicide technology has been widely used by farmers as the most cost-effective weed control measure, and development of new strategy to improve herbicide tolerance in plants is urgently needed. The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to agricultural technology for crop improvement. Here we identified three polyamine uptake transporter (PUT) genes in rice that are homologous to the Arabidopsis AtRMV1. We successfully demonstrate that CRISPR/Cas9-targeted mutagenesis of OsPUT1/2/3 greatly improves paraquat resistance in rice without obvious yield penalty. Therefore, manipulation of these loci could be valuable for producing transgene-free rice with improved herbicide resistance in future.
      PubDate: 2022-06-25
      DOI: 10.1007/s42994-022-00075-4
       
  • Characterization of two constitutive promoters RPS28 and EIF1 for studying
           soybean growth, development, and symbiotic nodule development

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      Abstract: Abstract Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits. Although several such promoters have been reported in soybean (Glycine max), few of them function at multiple growth and development stages and during nodule development. Here, we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28 (RPS28) and EUKARYOTIC TRANSLATION INITIATION FACTOR 1 (EIF1) are ideal for high expression of transgene. Through bioinformatic analysis, we determined that RPS28 and EIF1 were highly expressed during soybean growth and development, nodule development, and various biotic and abiotic stresses. Fusion of both RPS28 and EIF1 promoters, with or without their first intron, with the reporter gene β-GLUCURONIDASE (uidA) in transgenic soybean, resulted in high GUS activity in seedlings, seeds, and nodules. Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression, comparable to the soybean Ubiquitin (GmUbi) promoter. RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana. Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean, as well as in a wide variety of other plant species.
      PubDate: 2022-06-13
      DOI: 10.1007/s42994-022-00073-6
       
  • Expanding the range of CRISPR/Cas9-directed genome editing in soybean

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      Abstract: Abstract The CRISPR/Cas9 system has been widely applied for plant genome editing. The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif (PAM) sequences in the canonical form NGG and non-canonical NAG. Although these PAM sequences are extensively distributed across plant genomes, a broader scope of PAM sequence is required to expand the range of genome editing. Here we report the adoption of three variant enzymes, xCas9, SpCas9-NG and XNG-Cas9, to produce targeted mutation in soybean. Sequencing results determined that xCas9 with the NGG and KGA (contains TGA and GGA) PAMs successfully induces genome editing in soybean genome. SpCas9-NG could recognize NGD (contains NGG, NGA and NGT), RGC (contains AGC and GGC), GAA and GAT PAM sites. In addition, XNG-Cas9 was observed to cleave soybean genomic regions with NGG, GAA and AGY (contains AGC and AGT) PAM. Moreover, off-target analyses on soybean editing events induced by SpCas9 and xCas9 indicated that two high-fidelity Cas9 variants including eSpCas9 (enhanced specificity SpCas9) and exCas9 (enhanced specificity xCas9) could improve the specificity of the GGA PAM sequence without reducing on-target editing efficiency. These findings significantly expand the scope of Cas9-mediated genome editing in soybean.
      PubDate: 2022-06-01
      DOI: 10.1007/s42994-021-00051-4
       
  • Expanding the gene pool for soybean improvement with its wild relatives

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      Abstract: Abstract Genetic diversity is a cornerstone of crop improvement, However, cultivated soybean (Glycine max) has undergone several genetic bottlenecks, including domestication in China, the introduction of landraces to other areas of the world and, latterly, selective breeding, leading to low genetic diversity the poses a major obstacle to soybean improvement. By contrast, there remains a relatively high level of genetic diversity in soybean’s wild relatives, especially the perennial soybeans (Glycine subgenus Glycine), which could serve as potential gene pools for improving soybean cultivars. Wild soybeans are phylogenetically diversified and adapted to various habitats, harboring resistance to various biotic and abiotic stresses. Advances in genome and transcriptome sequencing enable alleles associated with desirable traits that were lost during domestication of soybean to be discovered in wild soybean. The collection and conservation of soybean wild relatives and the dissection of their genomic features will accelerate soybean breeding and facilitate sustainable agriculture and food production.
      PubDate: 2022-05-20
      DOI: 10.1007/s42994-022-00072-7
       
  • Elimination of an unfavorable allele conferring pod shattering in an elite
           soybean cultivar by CRISPR/Cas9

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      Abstract: Abstract Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still pod shattering-susceptible, limiting their regional and climatic adaptabilities. Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar, Huachun6 (HC6), and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1 (PDH1) gene, which exists in a significant portion of soybean cultivars. We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). In T2 progenies, several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits. The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma. Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars.
      PubDate: 2022-03-07
      DOI: 10.1007/s42994-022-00071-8
       
  • Correction to: PRMT6 physically associates with nuclear factor Y to
           regulate photoperiodic flowering in Arabidopsis

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      PubDate: 2021-12-24
      DOI: 10.1007/s42994-021-00066-x
       
  • The genetic and molecular basis for improving heat stress tolerance in
           wheat

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      Abstract: Abstract Wheat production requires at least ~ 2.4% increase per year rate by 2050 globally to meet food demands. However, heat stress results in serious yield loss of wheat worldwide. Correspondingly, wheat has evolved genetic basis and molecular mechanisms to protect themselves from heat-induced damage. Thus, it is very urgent to understand the underlying genetic basis and molecular mechanisms responsive to elevated temperatures to provide important strategies for heat-tolerant varieties breeding. In this review, we focused on the impact of heat stress on morphology variation at adult stage in wheat breeding programs. We also summarize the recent studies of genetic and molecular factors regulating heat tolerance, including identification of heat stress tolerance related QTLs/genes, and the regulation pathway in response to heat stress. In addition, we discuss the potential ways to improve heat tolerance by developing new technologies such as genome editing. This review of wheat responses to heat stress may shed light on the understanding heat-responsive mechanisms, although the regulatory network of heat tolerance is still ambiguous in wheat.
      PubDate: 2021-12-03
      DOI: 10.1007/s42994-021-00064-z
       
 
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