Subjects -> AGRICULTURE (Total: 981 journals)
    - AGRICULTURAL ECONOMICS (93 journals)
    - AGRICULTURE (680 journals)
    - CROP PRODUCTION AND SOIL (120 journals)
    - DAIRYING AND DAIRY PRODUCTS (30 journals)
    - POULTRY AND LIVESTOCK (58 journals)

AGRICULTURE (680 journals)                  1 2 3 4 | Last

Showing 1 - 200 of 263 Journals sorted alphabetically
aBIOTECH : An International Journal on Plant Biotechnology and Agricultural Sciences     Hybrid Journal   (Followers: 2)
Aceh International Journal of Science and Technology     Open Access   (Followers: 6)
Acta agriculturae Slovenica     Open Access   (Followers: 2)
Acta Agrobotanica     Open Access   (Followers: 3)
Acta Agronomica Hungarica     Full-text available via subscription   (Followers: 3)
Acta Agronomica Sinica     Full-text available via subscription   (Followers: 4)
Acta Aquatica Turcica     Open Access   (Followers: 1)
Acta Biologica Sibirica     Open Access   (Followers: 1)
Acta Fytotechnica et Zootechnica     Open Access   (Followers: 2)
Acta Scientiarum Polonorum Technica Agraria     Open Access   (Followers: 1)
Acta Scientiarum. Animal Sciences     Open Access   (Followers: 5)
Acta Scientifica Malaysia     Open Access   (Followers: 1)
Acta Technologica Agriculturae     Open Access   (Followers: 1)
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis     Open Access   (Followers: 2)
Acta Universitatis Sapientiae, Alimentaria     Open Access   (Followers: 2)
Advanced Research in Life Sciences     Open Access   (Followers: 2)
Advances in Agriculture     Open Access   (Followers: 14)
Advances in Agriculture & Botanics     Open Access   (Followers: 5)
Advances in Agronomy     Full-text available via subscription   (Followers: 20)
Advances in Horticultural Science     Open Access   (Followers: 4)
Advances in Life Science and Technology     Open Access   (Followers: 10)
Africa Research Bulletin: Political, Social and Cultural Series     Hybrid Journal   (Followers: 22)
African Journal of Food, Agriculture, Nutrition and Development     Open Access   (Followers: 15)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 12)
Agra Europe     Full-text available via subscription   (Followers: 3)
Agribusiness : an International Journal     Hybrid Journal   (Followers: 6)
Agric     Open Access   (Followers: 1)
Agricultura     Open Access   (Followers: 3)
Agricultura Tecnica     Open Access   (Followers: 2)
Agricultura Tropica et Subtropica     Open Access   (Followers: 2)
Agricultura, Sociedad y Desarrollo     Open Access   (Followers: 2)
Agricultural & Environmental Letters     Open Access   (Followers: 4)
Agricultural and Food Science     Open Access   (Followers: 14)
Agricultural Commodities     Full-text available via subscription   (Followers: 1)
Agricultural Economics     Open Access   (Followers: 6)
Agricultural Economics : The Journal of the International Association of Agricultural Economists     Hybrid Journal   (Followers: 60)
Agricultural History Review     Full-text available via subscription   (Followers: 14)
Agricultural Research     Hybrid Journal   (Followers: 10)
Agricultural Science     Full-text available via subscription   (Followers: 1)
Agricultural Sciences     Open Access   (Followers: 5)
Agricultural Systems     Hybrid Journal   (Followers: 35)
Agricultural Water Management     Hybrid Journal   (Followers: 50)
Agriculture     Open Access   (Followers: 3)
Agriculture     Open Access   (Followers: 7)
Agriculture & Food Security     Open Access   (Followers: 22)
Agriculture and Food Sciences Research     Open Access   (Followers: 10)
Agriculture and Human Values     Open Access   (Followers: 26)
Agriculture, Ecosystems & Environment     Hybrid Journal   (Followers: 65)
AGRIEAST : Journal of Agricultural Sciences     Open Access   (Followers: 2)
AgriEngineering     Open Access  
Agrinova (Agrotechnology Innovation)     Open Access   (Followers: 1)
Agriprobe     Open Access   (Followers: 1)
Agriscience     Open Access   (Followers: 1)
Agrisost     Open Access   (Followers: 1)
Agritech     Open Access   (Followers: 1)
AGRITROPICA : Journal of Agricultural Sciences     Open Access   (Followers: 1)
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan / Journal of Agricultural Sciences and Veteriner)     Open Access   (Followers: 5)
Agrivita : Journal of Agricultural Science     Open Access  
Agro Sur     Open Access  
Agro-Science     Full-text available via subscription   (Followers: 2)
Agroalimentaria     Open Access  
Agrociencia     Open Access  
Agrociencia Uruguay     Open Access  
Agroecological journal     Open Access  
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
Agronomía & Ambiente     Open Access   (Followers: 1)
Agronomía Costarricense     Open Access  
Agronomía Mesoamericana     Open Access  
Agronomía Tropical     Open Access  
Agronomie Africaine     Full-text available via subscription  
Agronomy     Open Access   (Followers: 12)
Agronomy Science     Open Access  
Agrosains : Jurnal Penelitian Agronomi     Open Access  
Agrosearch     Open Access  
Agrosintesa Jurnal Ilmu Budidaya Pertanian     Open Access  
Agrosystems, Geosciences & Environment     Open Access   (Followers: 4)
Agrotechnology Research Journal     Open Access  
Agrotekma : Jurnal Agroteknologi dan Ilmu Pertanian     Open Access  
Agrotrop : Journal on Agriculture Science     Open Access   (Followers: 1)
AL-Qadisiya Journal For Agriculture Sciences     Open Access  
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 6)
American Journal of Botany     Full-text available via subscription   (Followers: 18)
American Journal of Economics and Sociology     Hybrid Journal   (Followers: 37)
American Journal of Potato Research     Hybrid Journal   (Followers: 2)
American Journal of Rural Development     Open Access   (Followers: 5)
Analytical Science Advances     Open Access   (Followers: 1)
Animal - Open Space     Open Access   (Followers: 7)
Animal Diseases     Open Access   (Followers: 1)
Animal Microbiome     Open Access   (Followers: 1)
Annales des Sciences Agronomiques     Full-text available via subscription  
Annals of Agricultural Sciences     Open Access   (Followers: 1)
Annals of Arid Zone     Open Access   (Followers: 4)
Annals of Silvicultural Research     Open Access  
Annual Review of Resource Economics     Full-text available via subscription   (Followers: 11)
Applied Financial Economics Letters     Hybrid Journal   (Followers: 8)
Aquacultura Indonesiana     Open Access  
Arboricultural Journal : The International Journal of Urban Forestry     Hybrid Journal   (Followers: 6)
Archiva Zootehnica     Open Access  
Archives of Current Research International     Open Access  
Archivos de Zootecnia     Open Access   (Followers: 1)
ARO. The Scientific Journal of Koya University     Open Access  
Arthropod-Plant Interactions     Hybrid Journal   (Followers: 2)
Artificial Intelligence in Agriculture     Open Access   (Followers: 2)
Asia-Pacific Journal of Rural Development     Hybrid Journal   (Followers: 2)
Asian Economic Papers     Hybrid Journal   (Followers: 8)
Asian Food Science Journal     Open Access   (Followers: 1)
Asian Journal of Advances in Agricultural Research     Open Access  
Asian Journal of Agricultural and Horticultural Research     Open Access   (Followers: 1)
Asian Journal of Agricultural Extension, Economics & Sociology     Open Access   (Followers: 1)
Asian Journal of Agricultural Research     Open Access   (Followers: 6)
Asian Journal of Agriculture     Open Access  
Asian Journal of Medical and Biological Research     Open Access   (Followers: 3)
Asian Journal of Plant Sciences     Open Access   (Followers: 3)
Asian Journal of Research in Agriculture and Forestry     Open Access   (Followers: 1)
Asian Plant Research Journal     Open Access  
Asian Research Journal of Agriculture     Open Access   (Followers: 1)
Atatürk Üniversitesi Ziraat Fakültesi Dergisi     Open Access  
Australian Cottongrower, The     Full-text available via subscription  
Australian Economic Papers     Hybrid Journal   (Followers: 10)
Australian Economic Review     Hybrid Journal   (Followers: 4)
Australian Forest Grower     Full-text available via subscription   (Followers: 2)
Australian Forestry     Hybrid Journal   (Followers: 1)
Australian Grain     Full-text available via subscription  
Australian Holstein Journal     Full-text available via subscription   (Followers: 1)
Australian Journal of Agricultural and Resource Economics     Hybrid Journal   (Followers: 4)
Australian Journal of Agricultural Engineering     Open Access   (Followers: 1)
Australian Sugarcane     Full-text available via subscription  
Avances en Investigacion Agropecuaria     Open Access   (Followers: 1)
Bangladesh Agronomy Journal     Open Access  
Bangladesh Journal of Agricultural Research     Open Access   (Followers: 1)
Bangladesh Journal of Scientific Research     Open Access  
Berichte aus dem Julius Kühn-Institut     Open Access  
Berkala Ilmiah Pertanian     Open Access   (Followers: 1)
BHUMI : Jurnal Agraria dan Pertanahan     Open Access  
Bioagro     Open Access  
Biocatalysis and Agricultural Biotechnology     Hybrid Journal   (Followers: 3)
Biochar     Hybrid Journal   (Followers: 1)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodiversity     Hybrid Journal   (Followers: 25)
Biodiversity : Research and Conservation     Open Access   (Followers: 28)
BIOFIX Scientific Journal     Open Access  
Biological Agriculture & Horticulture : An International Journal for Sustainable Production Systems     Partially Free   (Followers: 10)
Biosystems Engineering     Hybrid Journal   (Followers: 8)
Biotecnología en el Sector Agropecuario y Agroindustrial     Open Access  
Boletín Semillas Ambientales     Open Access  
Botanica Orientalis : Journal of Plant Science     Open Access  
British Poultry Science     Hybrid Journal   (Followers: 6)
Buletin Peternakan : Bulletin of Animal Science     Open Access   (Followers: 1)
Buletin Veteriner Udayana     Open Access   (Followers: 1)
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca : Food Science and Technology     Open Access   (Followers: 1)
CABI Agriculture and Bioscience     Open Access   (Followers: 2)
Caderno de Ciências Agrárias     Open Access  
Cahiers Agricultures     Open Access  
California Agriculture     Open Access   (Followers: 1)
Cambridge Journal of Economics     Hybrid Journal   (Followers: 78)
Canadian Water Resources Journal     Hybrid Journal   (Followers: 18)
Capitalism Nature Socialism     Hybrid Journal   (Followers: 21)
Caraka Tani : Journal of Sustainable Agriculture     Open Access  
Ceiba     Open Access  
Central European Forestry Journal     Open Access   (Followers: 1)
Cereal Chemistry     Full-text available via subscription   (Followers: 4)
CESifo Economic Studies     Hybrid Journal   (Followers: 23)
Ceylon Journal of Science     Open Access  
Chemical and Biological Technologies for Agriculture     Open Access   (Followers: 1)
Chilean Journal of Agricultural & Animal Sciences     Open Access  
Chilean Journal of Agricultural Research     Open Access   (Followers: 1)
Ciencia e Investigación Agraria     Open Access  
Ciência e Técnica Vitivinícola     Open Access  
Ciencia forestal en México     Open Access   (Followers: 1)
Ciencia, Tecnología y Salud     Open Access  
Científic@ : Multidisciplinary Journal     Open Access  
COCOS : The Journal of the Coconut Research Institute of Sri Lanka     Open Access   (Followers: 1)
Coffee Science     Open Access  
Cogent Food & Agriculture     Open Access  
Competition & Change     Hybrid Journal   (Followers: 12)
Compost Science & Utilization     Hybrid Journal  
Computers and Electronics in Agriculture     Hybrid Journal   (Followers: 7)
Corpoica Ciencia y Tecnología Agropecuaria     Open Access  
CSA News     Hybrid Journal   (Followers: 1)
Cuadernos de Desarrollo Rural     Open Access  
Cultivos Tropicales     Open Access  
Cultura Agronômica : Revista de Ciências Agronômicas     Open Access  
Cultural Geographies     Hybrid Journal   (Followers: 20)
Cultural Sociology     Hybrid Journal   (Followers: 34)
Cultural Studies - Critical Methodologies     Hybrid Journal   (Followers: 17)
Cultural Studies of Science Education     Hybrid Journal   (Followers: 11)
Cultural Trends     Hybrid Journal   (Followers: 13)
Culture, Agriculture, Food and Environment     Hybrid Journal   (Followers: 22)
Culture, Agriculture, Food and Environment     Hybrid Journal   (Followers: 8)
Current Agricultural Science and Technology     Open Access  
Current Agriculture Research Journal     Open Access  
Current Applied Science and Technology     Open Access  
Current Protocols in Plant Biology     Hybrid Journal   (Followers: 2)
Current Research in Dairy Sciences     Open Access   (Followers: 3)
Diatom Research     Hybrid Journal   (Followers: 3)
Dinamika Pertanian     Open Access  
Dissertationen aus dem Julius Kühn-Institut     Open Access  
Dossiers Agraris     Open Access  
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)     Open Access  
Ecological Applications     Full-text available via subscription   (Followers: 144)

        1 2 3 4 | Last

Similar Journals
Journal Cover
AGRIEAST : Journal of Agricultural Sciences
Number of Followers: 2  

  This is an Open Access Journal Open Access journal
ISSN (Online) 1391-5886
Published by Sri Lanka Journals Online Homepage  [71 journals]
  • Rooting performance of in vitro microshoots of strawberry (Fragaria x
           ananassa Duch.) as influenced by plant growth regulators

    • Abstract: This work was done to study the rooting performance of strawberry cv sweet Charlie under in vitro conditions. Healthy microshoots were collected from in vitro plantlets (1.0-1.5 cm long) grown in hormone free basal MS medium and placed in MS media containing BAP (0-1.0 mg/l) and (IBA 0-1.0 mg/l) for shoot elongation and root proliferation. The results showed that the higher number of newly formed shoots was observed in the medium containing 0.5 mg/l BAP and 0.5 mg/l IBA. The shoot and root lengths of newly formed shoots were high (>4.5 cm) in hormone free medium and also media with 0.5-1.0 mg/l IBA than the other tested media. Further, it was noted that more than ten roots per plantlet were recorded in MS media containing 0-1.0 mg/l IBA and also there was no significant variation (P>0.05) in number of roots between them. MS media supplemented with 0-1 mg/l IBA concentrations could be used for rooting of strawberry microshoots under in vitro conditions. Published on 2021-12-28 08:01:27
       
  • A retrospective analysis in the conservation of selected sites with
           Salacia species in the Southern Province of Sri Lanka

    • Abstract: The genus Salacia has high importance as it contains a medicinal value. Cultivation of Salacia species has become an attractive, profitable industry over the past few years mainly due to an increase in its demand and market value. Due to overexploitation, the majority of these bears threatened will become extinct in the future. Hence, the conservation plan for Salacia species was presented to conserve Salacia species. Data were collected from relevant respondents from the Ayurvedic Department, Ayurvedic industries, and Department of Agriculture and stakeholders of the value chain using focus group interviews and discussions from May to July in 2019.  Five options for recovery of Salacia species in the selected site were decided. Manage Salacia species on forest patches (option 2), protect plants of Salacia species at selected sites to correspond to the full range of remaining genetic diversity (option 3), and maintain Salacia species in cultivation (option 5) were chosen as the best options for the recovery of Salacia species in the selected sites. To manage these species on forest patches, protect plants at sites chosen to correspond to the full range of remaining genetic diversity, and maintain these species in cultivation, plans including their outcomes were decided. These suggestions, if suitably implicated, will go a long way forward conserving this invaluable herbal wealth. Published on 2021-12-28 00:00:00
       
  • Effect of pruning height on growth and cut flower production of rose (Rosa
           hybrid L.) variety ‘White Success’

    • Abstract: Pruning is an inevitable practice in rose cultivation which stimulates the development, flowering and longevity of the cut roses. Optimum pruning height of rose var. ‘White Success’ was not identified yet. Thus, the present experiment was performed to estimate the consequences of diverse pruning heights on growth and flower production of rose var. ‘White Success’ at the Regional Agricultural Research and Development Centre, Bandarawela, Sri Lanka from January to April 2019. The experiment was arranged according to randomized complete block design with four replications. One-year-old budded rose plants were hard pruned at 15 cm from the ground level before the commencement of the experiment and allowed to grow for a period of one month. Eight treatments were defined based on different pruning heights. Analysis of variance was performed to decide the treatment effects on measurements. Results revealed that pruning was positively influenced the growth and flowering of roses than non-pruned plants. The highest performances in measured parameters were observed in pruning shoots from 15 cm above the bud union. According to the results of the present study, it could be concluded that growth and the flowering of roses could be increased by pruning practices. Pruning of new stems at 15 cm above the bud union is the best method to get higher production of quality flowers from “White success”. Published on 2021-12-28 00:00:00
       
  • The occurrence of Enterobacteriaceae in the well water of Nallur
           Divisional Secretariat area of Jaffna district, Sri Lanka

    • Abstract: The access for safe drinking water has become a challenge to human at present due to contamination with the different sources of pollution such as domestic waste, animal excreta and latrine leakage. Therefore, this study was carried out with the focus of determining the microbial quality of dug well water from a highly populated peri-urban area (Nallur Divisional Secretariat) of the Jaffna district in Sri Lanka. The water samples from 20 dug wells were collected from the study area and the information particularly the distance of well from the toilet septic tank, sanitary conditions of well and the surroundings were gathered. The total bacterial count was determined and different bacterial species were identified on Xylose Lysine Deoxycholate (XLD) agar.The t-test was performed to identify the factor/s that significantly influences the total bacterial count. A correlation analysis was performed between the distance of well from septic tank and bacterial count. Nine different bacterial species, that belong to the family Enterobacteriaceae, were identified; Klebsiella, Enterobacter, Escherichia, Salmonella, Shigella, Citrobacter, Yersinia, Serratia and Acinetobacter. Of the 72 bacterial isolates from 20 wells, 37 belong to the coliform group indicating recent faecal contamination. The total bacterial count has an inverse correlation with the distance of the toilet from the well (R2=0.506). Presence of pathogenic bacteria in the well water in the study is a great threat for human health and therefore, regular measures need to be taken to disinfect the water. Published on 2021-12-28 00:00:00
       
  • Sustainable organic waste management and nutrients replenishment in the
           soil by vermicompost: A review

    • Abstract: With the rapid growth of global population, food production and waste management are considered to be two major challenges faced by people in 21st century. Though application of chemical fertilizers enhances crop growth, vigor and yield thereby meeting the food security, it depletes the quality of environment and ecosystems. Recycling of organic wastes is considered to be a feasible solution which could mitigate the issues of waste management while uplifting the status of soil health and nutrients. The objective of this article is to reviews the currently available literature on vermicomposting as a tool for recycling of different organic waste materials and subsequent use as organic amendment to improve soil properties and as organic fertilizer to enhance growth and yield of various crops. Furthermore, models were proposed to improve education on vermicomposting as a solution for organic waste management among farmers, students, government offices and general public and develop as a community based micro-enterprise. Vermicomposting is defined as a process where earthworms convert organic wastes into humus like materials. It has been investigated worldwide taking its practical significance into account. Vermicompost is found to be a good source of plant macro nutrients, micro nutrients and growth regulators, including humic acid, indole acetic acid and kinetin. Microbes live in the alimentary canal of the earthworms improve the quality of vermicast hence vermicompost. Furthermore, vermicompost could absorb heavy metals in soil and water while eliminating of human pathogens such as Salmonella spp, fecal coliforms, Shigella spp, Enterobacter aerogenes, Enterobacter cloacae and eggs of helminthes. Reviewed literature indicated the potential of vermicomposting to be used as an efficient technique for bioconversion of organic wastes into a valuable organic fertilizer. Furthermore, the present review suggests vermicompost production as a micro-enterprise which could be used to uplift the livelihood of the resource poor farmers through economic empowerment while assuring sustainable crop production. Published on 2021-12-28 00:00:00
       
  • Bioconversion of perennial weeds of Chromolaena odorata (L.) R.M. King &
           H. Rob., Sida rhombifolia L., and Lantana camara L. by vermicomposting
           process

    • Abstract: Bioconversion of weeds through vermicomposting is getting momentum in sustainable organic farming, whilst mitigating negative impacts on the environment. The present study was conducted to find out the effect of three perennial weed species and the different ratio between weed to cow dung on the efficacy of Eisenia fetida and the physicochemical properties of vermicompost produced. The experiment was set up as a two-factor factorial completely randomized design with three replicates and 100% of cow dung was maintained as the control experiment. Factor one of the experiment was the weed species (Three weed species; Chromolaena odorata, Sida rhombifolia and Lantana camara) and factor two was the different ratio between weed and cow dung when preparing substrate by weight (four ratios; 100% weed, 75% weed + 25% cow dung, 50% weed + 50% cow dung, 25% weed + 75% cow dung). The results revealed that the interaction effect between weed species and the ratio of weed to cow dung on live biomass of earthworms (P<0.001), weight (P<0.001) and total dissolved solids (P<0.05) of the vermicompost was significantly different. The salinity and electrical conductivity of the vermicompost was significantly different among the main factors of weed species (P<0.01) and the ratio of weed to cow dung (P<0.01). The pH value of the vermicompost was significantly different only among the weed species (P<0.01). A significant difference was found between treatment combinations for the colour (P<0.01) of the vermicompost. However, the odour of the vermicompost harvested in different treatment combinations was not significantly different. The treatment combination of 50% Lantana camara and 50% cow dung performed better than the control for the growth and reproduction of earthworms. However, none of the treatment combinations performed better than the control for the weight of vermicompost. Furthermore, the pH of the vermicompost was not significantly different from the control. The treatment combination of 25% Chromolaena odorata and 75% cow dung was significantly different from the control on electrical conductivity, total dissolved solids and salinity of the vermicompost. According to the results of the experiment, the treatment combination of 50% cow dung and 50% Lantana camara L. by weight showed the highest efficacy on Eisenia fetida. Therefore, it can be concluded that the bioconversion of perennial weeds of Chromolaena odorata, Sida rhombifolia and Lantana camara were possible and 50% cow dung and 50% Lantana camara L. by weight produced the best quality vermicompost. Furthermore, it can be suggested that the perennial weed species of Lantana camara could be used to produce vermicompost effectively than the other weed species used in the study. Bioconversion of weed substrate without mixing of cow dung was not successful. Better performances of Eisenia fetida can expect by feeding partially degraded weed substrate. Published on 2021-12-28 00:00:00
       
  • Effect of different irrigation intervals on growth and yield of chilli
           crop grown in sandy soil

    • Abstract: A study was carried out to evaluate the effect of different irrigation intervals on growth and yield performance of chilli grown under dry weather condition in sandy soil of Batticaloa district. The experimental setup was a Randomized Complete Block Design of four treatments and five replicates. The treatments applied were; T1 (irrigated daily as control), T2 (at 3 days irrigation interval), T3 (at 5 days interval for first 12 weeks and then with 6 days interval) and T4 (at 7 days irrigation interval). Growth and yield attributes such as plant height, leaf area, number of branches, fresh and dry weight of plant shoots and roots and yield were recorded. The results revealed that, higher irrigation frequency had shown higher growth rate on crop characteristics such as plant height (64.3 cm), leaf area (28.48 cm2), shoot fresh weight (112.09g), shoot dry weight (28.01g), root fresh weight (12.58g), root dry weight (3.85g) etc. Further, the daily irrigation significantly increased the yield of chilli, whereas irrigation at 3 days and 5 days did not influence significantly. The yield was significantly lower for treatment with 7 days irrigation interval. The yield reduction was by 45.4% for 7 days irrigation interval from daily irrigated plots which was significantly higher. Study concluded that, daily irrigation shows highest desirable output for most of the parameters. Non-significant differences in growth characteristics were noticed for the T2 and T3, while 7 days irrigation interval (T4) shows the lowest performance on the growth characteristics of chilli. Therefore, daily irrigation is most suitable for sandy soils under dry weather conditions in this study area. Published on 2021-09-16 00:00:00
       
  • Purification and characterization of cellulase from Aspergillus unguis
           isolated from decaying coconut wood

    • Abstract: The insufficiency of available natural fossil fuels for increasing human population, air pollution due to partial combustion of fossil fuel and the release of greenhouse gasses have led great attention on the usage of cellulase like enzymes to hydrolyze lignocellulosic substances to produce bioethanol. Thermostability and kinetic properties of cellulases need to be studied before deciding the eligibility of the enzymes for their potential applications. This study was aimed to purify the crude cellulase from Aspergillus unguis and to characterize the purified cellulase. When the crude enzyme from Aspergillus unguis isolated from decaying coconut wood, was subjected to fractional precipitation and dialysis by the addition of 80% saturated (NH4)2SO4, the recovery of cellulase was 83.9 % showing specific activity of 16386.43Umg-1protein. The dialyzed enzyme was added to a column packed with DEAE-Sepharose equilibrated with 0.01M sodium phosphate buffer (pH 7.0) and unbound proteins were washed with the same buffer. The specific activity of cellulase was increased from 3228 to 37071 Umg-1 protein, which was 11.5-fold higher than that of the crude cellulase with 67.6 % yield. The molecular weight of the purified cellulase was determined as 50 KDa using Poly Acrylamide Gel Electrophoresis (SDS-PAGE). When the activity of purified cellulase was measured at different temperatures ranging from 40oC to 900C at neutral pH, the optimum temperature for the activity of the purified cellulase enzyme was 700C. The pH was optimized as 5.0 for the cellulase at 700C. Michaelis constant for the purified cellulase to soluble cellulose by Lineweaver-Burk Plot was 4.45 ×10-2 moldm-3 and Vmax was 28.5714 mgml-2mins-1 with 10 gL-1 of cellulose substrate, at pH 5.0 and 700C. The purified cellulase was stable for at least 90minutes at pH 5.0 and 700C and the half-life obtained for this enzyme was significantly higher at 700C than any other temperatures. Therefore, the crude cellulase from Aspergillus unguis can be purified by ammonium sulphate precipitation and DEAE-sepharose ion exchange chromatography. The thermostable acidic cellulase from Aspergillus unguis could be apotential candidate for diverse industrial applications. Published on 2021-09-16 00:00:00
       
  • Purification of xylanase from Bacillus pumilus using Eudragit S-100 and
           optimization of conditions

    • Abstract: Soluble-insoluble polymers have considerable applications in the enzyme purification and these polymers precipitate from the solution by changing the pH. Eudragit S-100, nontoxic polymer of methacrylic acid and methyl methacrylate, has been used in affinity precipitation. The study describes the purification of xylanase from Bacillus pumilus by three phase partitioning (TPP) method and precipitation (PT) method using Eudragit S-100 and to optimize these methods to improve the purification fold. In the TPP method, 14.6 U mL-1 of xylanase activity was obtained with a specific activity of 31.74 U mg protein-1. The recovery of the enzyme was 52.24 % with 1.79-fold purification. In the PT method, 15 U mL-1 of xylanase activity was obtained with the specific activity of 33.4 U mg protein-1. The recovery of the enzyme was 52.18% with 1.72-fold purification. Since there is no significant difference in the purification folds of both these methods and to improve the purification fold, the conditions of these methods were optimized. When different concentrations of (NH4)2SO4 were used, xylanase with significantly higher specific activity (33.74 U mg protein-1) was precipitated with 50% of (NH4)2SO4 saturation. Among the different Eudragit concentrations used, 40gL-1 Eudragit S-100 yielded significantly higher xylanase activity (18.9 UmL-1) than the other Eudragit concentrations. When the spent medium was treated with 40gL-1 Eudragit S-100 and Eudragit bound xylanase was eluted with different concentrations of NaCl, significantly higher activity of xylanase (17.9UmL-1) was eluted with of 0.3 M NaCl. When TPP method of purification was done under optimized conditions (with 50% (NH4)2SO4 saturation and usage of 0.3 M NaCl for elution), 17.87U mL-1 of xylanase activity was obtained with a specific activity of 38.1 U mg protein-1and the xylanase enzyme yield and purification fold were significantly increased to 63.41% and 2.01 than the non-optimized TPP method. When PT method of purification was done under optimized conditions (with 40gL-1 Eudragit S-100 and usage of 0.3 M NaCl for elution), 18.96 U mL-1 of xylanase activity with the specific activity of 43.09 U mg protein-1 was obtained and the xylanase yield and the purification fold were significantly increased to 69.67% and 2.31 respectively than the non-optimized PT method. Since the purification fold of the optimized PT method is significantly higher than that of optimized TPP method, optimized PT method could be recommended for the purification of xylanase from Bacillus type bacteria. When the purified xylanase was subjected to poly acrylamide gel electrophoresis, it gave a single band and the molecular weight of the purified xylanase was determined as 55.4 kDa. Published on 2021-09-16 00:00:00
       
  • A case study in the utilization of the underutilized crop; Salacia species
           in lowland wet zone of Sri Lanka

    • Abstract: A large population in developing countries depends on herbal drugs for their health care needs. We recognized a central gap in recent ethno-pharmacological research in Salacia species, which impacts factors applicable for a sustainable, equal socio-cultural, and safe supply of herbal medicines. The study was conducted at the lowland wet zone in Sri Lanka to understand the current environmental, economic, and social status of various Salacia species. Data were collected from using a mixed-method approach with a general review of the literature in a systematic online search with a hand search of bibliographies, face to face interviews, and discussions with respondents from March to May 2019. The data were collected from a total of 100 respondents that consisted of cultivators (60), collectors (2), traders (10), processors (5), and consumers (23). The survey results revealed that Salacia species in the wild are illegally harvested by unskilled persons for different purposes and economic benefit through illegal sales. It also revealed various issues such as the lack of knowledge about Salacia species, harvesting time, unavailability of desired Salacia species, and government restrictions or bans on wild collection faced by Salacia species collectors in the region had occurred in this field. This suggests that there is a need to develop practical and economically sound strategies for the efficient utilization of Salacia species to improve the condition of marginalized communities, which is a process that should eventually lead the country towards greater economic stability. Published on 2021-09-16 00:00:00
       
 
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