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Jurnal Sain Veteriner
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This is an Open Access Journal

Open Access journal
ISSN (Print) 0126-0421 - ISSN (Online) 2407-3733
Published by Universitas Gadjah Mada

[46 journals]

Efek pemberian maserasi taoge (Vigna radiata) terhadap profil hematologi
dan biokimia darah tikus betina
- Authors: andriyanto andriyanto Andriyanto
  Pages: 244 - 250
  Abstract: Taoge memiliki berbagai manfaat farmakologis yang berpotensi besar untuk meningkatkan kesehatan. Penelitian ini dilakukan untuk mengeksplorasi keamanan dari konsumsi maserasi taoge melalui pengamatan terhadap profil hematologi dan biokimia darah. Sebanyak 15 ekor tikus betina galur Sprague Dawley berumur 8 minggu, memiliki bobot badan 220-230 g, dan belum pernah bunting dikelompokkan secara acak ke dalam 3 kelompok perlakuan (n=5 tikus), yaitu kelompok kontrol (diberi air mineral); kelompok maserasi taoge 1%; dan kelompok maserasi taoge 5%. Pemberian maserasi dilakukan melalui air minum dan dilaksanakan selama 20 hari. Hasil menunjukkan bahwa secara umum perlakuan maserasi taoge tidak memberikan pengaruh nyata (P>0.05) terhadap profil hematologi dan biokimia darah tikus percobaan. Terdapat peningkatan signifikan (P>0.05) pada kadar hemoglobin pada kelompok maserasi taoge 1% dibandingkan dengan kontrol yang lebih tinggi dari nilai normal. Hal yang serupa juga terjadi pada nilai neutrofil yang lebih tinggi dari nilai normal namun tidak berbeda nyata dengan kontrol (p>0.05). Selain keduanya, semua parameter menunjukkan nilai yang tidak berbeda nyata dibandingkan dengan kelompok kontrol dan berada pada rentang nilai normal.. Hal ini mengindikasikan bahwa pemberian maserasi tauge tidak memberikan efek negatif dan toksik pada dosis 1 dan 5% terhadap profil darah serta fungsi organ hati dan ginjal.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.73351
  Issue No: Vol. 40, No. 3 (2022)
Open Reduction Internal Fixation of Sacroiliac Fraktur-Luxation in Dog
Using Plate 2.0 with screw 2.0 mm cortical self-tapping and 2.7 mm
cortical non self-tapping
- Authors: Erwin Erwin
  Pages: 251 - 255
  Abstract: The Mixed Shih-Tzu dog, 3 years old and 4.3 kg body weight, had clinical signs of unable to stand, both rear limbs suffer from diplegic lameness so that they are only able to rely on both forelimbs. The owner said that her dog is traumatized by a four-wheeled vehicle. The results of blood profile before surgery showed physiological, only an increase in leukocyte cells 18.83 x103/µL. The radiographic examination with a ventral-dorsal position shows oblique fractures in the pelvic dexter and dislocation of the pelvic sacroiliac sinister bone. The method of handling used was open reduction internal fixation (ORIF) using a 2.0 veterinary cuttable plate (VCP) bone plate with a 2.0 mm cortical self-tapping screw for pelvic fractures and 2.7 mm cortical non-self-tapping for sacroiliac dislocations. The dog walked and showed a good progress in one week after ORIF, based on clinical and post-operative radiographic examinations, sacroiliac fracture-dislocation fixation using VCP with 2.0 mm self-tapping screw cortical for pelvic fracture and 2.7 mm non self-tapping for sacroiliac dislocation provides excellent stabilization and no visible complications.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.53112
  Issue No: Vol. 40, No. 3 (2022)
Combination of Wild ginger, Honey, and Probiotics as Growth Prometer
Candidates : An in Vitro Study
- Authors: Marlin Cindy Claudya Malelak
  Pages: 256 - 267
  Abstract: Growth Promoter Antibiotics are used to prevent disease and promote growth and production in poultry. Repeated admnistration of feed can have a microorganic resistance effect, accumulation of antibiotic residues in animal and environmental products and imbalance of normal microflora in the intestine. The antibacterial and carbohydrate content of some natural ingredients can be potential as a replacement candidate for AGP. This study aims to determine the role of a combination of wild ginger, honey, and probiotics (Bacillus subtilis and Lactobacillus acidophilus) as AGP candiate in Vitro. The antibacterial activity of the combination of wild ginger and honey against pathogens (E. coli) and their use against probiotics was tested by disk diffusion method, while the calculation of optical density values to determine the minimum inhibitory concentration and minimum bactericidal concentration was carried out on E. coli. The ability of inhibition of probiotics against pathogens is also done by the disk diffusion method. The disk diffusion test results showed the best combination was extract of 25% wild ginger aquades + 100% Lombok honey with inhibition zone diameter (8,53 ±, 03). Optical density values indicate this combination is able to inhibit and kill E. coli (DO 0,00 ±, 002) and support B. subtilis (DO 0,18 ±, 002) and L. acidophilus (DO 0.25 ±, 005) significantly better than positive control. MIC value of wild ginger aquades extract and honey combination against E. coli is wild ginger aquades extract 3.13% + Lombok honey 25%, while MBC value is wild ginger aquades extract 6,25% + Lombok honey 25%. The combination of B. subtilis and L. acidophilus showed the largest inhibitory zone diameter against E. coli (7,30 ±, 02 mm) compared to individual colonies. The combination of wild ginger and honey, in addition to inhibiting also able to kill pathogens and support the growth of probiotics, so this formula can be used as one of the replacement candidates for AGP.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.53256
  Issue No: Vol. 40, No. 3 (2022)
Study of Genetic Diversity of Native Indonesian Catfish Based on
Nucleotide Sequences of the ND1 Gene
- Authors: Rini Widayanti
  Pages: 268 - 275
  Abstract: One of the native catfish to Indonesia is the baung fish (Hemibagrus). Baung fish are found in rivers in Sumatra, Kalimantan and Java. The catfish population is declining, thus conservation is needed to prevent extinction. To conduct an effective conservation efforts, molecular studies are needed to confirm the fish species from the three islands. Mitochondrial DNA is one of the markers that is often used to see the lineage and kinship of animals for conservation purposes. The purpose of this study is to determine the genetic diversity of the NADH Dehydrogenase Subunit I (ND1) gene catfish from each of these locations, can be used as a genetic marker. Samples were obtained from their natural habitat, namely Magelang (5), Palembang (3), Riau (2), Samarinda (2), 2 from Sintang, and 3 from Banjarmasin. The DNA of the fish sample was then isolated and then used as a template for amplification of DNA fragments using PCR techniques. Amplikon (PCR product) was then purified by gel extraction and then sequenced to determine the DNA sequence. The potential of DNA sequences as catfish genetic markers was proven by analyzing genetic diversity between species using the MEGA version 7.0 program (Kumar et al., 2016). The sequencing results 972 nucleotides composing the ND gene, and found differences in 268 nucleotide sites and 47 amino acid sites. Based on the nucleotide sequence of the ND1 gene, catfish from the Progo river (Magelang, Central Java), the Musi river (Palembang, Sumatra), the Kampar river (Riau), the Kapuas river (Sintang, Kalimantan), the Martapura river (Banjarmasin), the Mahakam river (Kalimantan) is included in the Hemibagrus sp.; catfish from the Elo river (Magelang, Central Java) belongs to the genus Mystus sp.; catfish from the Bengawan Solo river (Bojonegoro, East Java) belongs to the Pangasius sp.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.62755
  Issue No: Vol. 40, No. 3 (2022)
POTENSI ANESTETIKA KET-A-XYL® PADA KUCING JANTAN DOMESTIK DI
YOGYAKARTA INDONESIA
- Authors: Maria Angelica Maryatmo, Antasiswa Windraningtyas Rosetyadewi, Aria Ika Septana, Agustina Dwi Wijayanti
  Pages: 276 - 283
  Abstract: Ket-A-Xyl® (Ket-A-Xyl® 20 ml, AgroVet, Peru) merupakan sediaan anastetika jadi yang telah banyak dipasarkan di Indonesia untuk induksi anastesi pada anjing. Studi ini menguji penggunaan obat Ket-A-Xyl® untuk kastrasi pada kucing. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian Ket-A-Xyl® terhadap parameter fisiologis kucing jantan domestik. Sebanyak 53 ekor kucing jantan domestik dengan berat badan berkisar 1,5 – 5,68 kg dipuasakan selama 8 jam kemudian ditimbang untuk menentukan dosis anestesi. Kucing diinjeksikan sediaan Ket-A-Xyl® secara intramuskular, kemudian diamati dan dicatat perubahan fisiologisnya. Demikian juga untuk onset dan durasi obat dicatat saat kucing memasuki stadium anestesi I hingga tahap recovery. Analisis data parameter fisiologis dilakukan menggunakan aplikasi SPSS dengan uji paired sample t-test. Kelompok yang dibandingkan dalam uji paired sample t-test tersebut adalah kelompok data fisiologis kucing pada tahap stadium III dengan tahap recovery. Hasil analisis data menunjukkan bahwa sediaan Ket-A-Xyl® menimbulkan efek yang signifikan terhadap frekuensi napas (p<0,05), tetapi tidak pada suhu dan frekuensi pulsus. Rata-rata onset dan durasi obat Ket-A-Xyl® menunjukkan waktu yang lebih singkat dibanding studi sebelumnya yang menggunakan obat atropin-ketamin-xylazin. Sediaan anastesi Ket-A-Xyl® berpotensi sebagai obat anestetika yang baik pada kucing domestik karena lebih efisien, onset dan durasi singkat, serta tidak menimbulkan respon fisiologis yang fatal selama penggunaannya.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.69624
  Issue No: Vol. 40, No. 3 (2022)
Pendeteksian secara Otomatis Telur Cacing Haemonchus contortus menggunakan
Algoritma YOLOv3
- Authors: Ridi Arif, Elok Budi Retnani, Fadjar Satrija
  Pages: 284 - 289
  Abstract: Infeksi Haemonchus contortus atau haemonchosis umumnya terjadi pada ruminansia kecil seperti domba. Haemonchus contortus adalah spesies yang paling patogenik pada ruminansia kecil dan berhabitat di abomasum. Hewan yang terinfeksi Haemonchus contortus secara berangsur-angsur akan mengalami anemia karena aktivitas cacing yang menghisap darah. Selain menimbulkan anemia, domba akan mengalami penurunan bobot badan akibat penurunan daya cerna. Infeksi tersebut dapat berjalan secara akut maupun kronis dan dalam kondisi tertentu dapat juga mengakibatkan kematian pada hewan. Oleh karena itu dibutuhkan perangkat yang efektif dan efisien untuk mengindentifikasi keberadaan telur cacing Haemonchus contortus sebagai alat deteksi cepat telur cacing. Tujuan dari penelitian ini adalah membuat perangkat cerdas berbasis Algoritma YOLOv3 yang mampu mendeteksi dan mengidentifikasi telur cacing Haemonchus contortus secara cepat. Penelitian ini menggunakan Algoritma You Only Look Once (YOLO) versi 3 yang merupakan algoritma yang dikembangkan untuk membantu mendeteksi objek secara realtime. Algoritma YOLO dijalankan dalam framework aplikasi anaconda dengan menggunakan pycharm dan aplikasi OPENCV. Identifikasi telur Haemonchus contortus secara otomatis berhasil dilakukan dengan proses tagging pada dataset dan membuat file weight training bagi YOLO. Hasil uji coba menggunakan mikroskop cahaya dan smartphone menunjukan bahwa bahwa Algoritma YOLO mampu mengidentifikasi telur Haemonchus contortus dengan nilai confidence lebih dari 90%. Penggunaan perangkat Dino-Lite yang terhubung pada mikroskop cahaya menunjukan algoritma YOLO tidak dapat berjalan karena adanya enkripsi pada perangkat tersebut. Otomatisasi pendeteksian telur Haemonchus contortus dapat dilakukan dengan memanfaatkan Algoritma YOLOv3 yang dibantu dengan hardware berupa laptop dan smartphone android serta memiliki kemampuan identifikasi dengan tingkat akurasi yang tinggi.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.71945
  Issue No: Vol. 40, No. 3 (2022)
Diagnosis dan Pengobatan Babesiosis pada Anjing Shih-Tzu di Klinik
Lilipoet, Yogyakarta, Indonesia
- Authors: Nabilah Putroe Agung
  Pages: 290 - 297
  Abstract: Dogs are one of the pet animals that have a loyal nature and are widely cared for in the community. It is not uncommon for dogs to often play in parks or outdoor environments that are surrounded by grass to play with their owners. The grass is a breeding ground for ticks, which can be vectors of disease carriers for dogs. Babesiosis is a disease caused by Babesia sp. and is transmitted by tick vectors. In this paper, we will discuss the clinical condition, diagnosis, and treatment of one dog with babesiosis at the Lilipoet clinic, Yogyakarta. A Shih-Tzu dog has clinical symptoms of fever with a temperature of 39.9oC, decreased appetite, and has a history of yellow vomiting, and found one tick. Blood was collected for microscopic examination of blood smears, blood hematologic, and molecular examination. Hematologic examination of the blood showed the value of thrombocytopenia with a value of 32 x 103/µL. Molecular examination with Polymerase Chain Reaction (PCR) showed positive results of Babesia sp. with visible DNA bands at 490bp. Treatment was carried out by giving one dose of antiprotozoal diminazen aceturate 3.5 mg/kg BW and doxycycline 10mg/kg BW once a day for 14 days.
  PubDate: 2022-12-24
  DOI: 10.22146/jsv.74202
  Issue No: Vol. 40, No. 3 (2022)
Presence of Escherichia coli O157:H7 in Chicken Meat at Traditional
Markets in Pangkalpinang City
- Authors: Ahmad Nurhakim
  Pages: 298 - 306
  Abstract: This study was aimed to identify the presence of E. coli O157:H7 and analyze the association between factors and frequency of E. coli in chicken meat sold at Kaget Market, Pagi Market, and Pembangunan Market in Pangkalpinang City. A total of 30 chicken meat samples were collected proportionally using simple random sampling. This study was used a cross sectional method. The examination of E. coli was carried out based on SNI 2897:2008 concerning The Testing Method of Microbial Contamination in Meat, Eggs, and Milk and Their Processed Products. The E. coli O157 was confirmed with O157 latex agglutination test and H7 antiserum test. The data were analyzed using a descriptive test and risk factors were analyzed using chi-square and odds ratio. The results showed that E. coli O157:H7 was found in 2 of 3 samples (66,7%) isolated from Kaget Market. The significant risk factors (P<0,005) for the presence of E. coli were traders with age of more 45 years, kiosk distance of less than 5 meters from the highway, and washing hands using unclean water. The presence of E. coli O157:H7 will be a threat for consumer health.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.71162
  Issue No: Vol. 40, No. 3 (2022)
Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap
Peningkatan Kualitas Spermatozoa Sapi Bali Pengaruh Pemberian Gonadotropin
Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi
Bali
- Authors: Tongku Nizwan Siregar
  Pages: 307 - 313
  Abstract: Penelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.65067
  Issue No: Vol. 40, No. 3 (2022)
ISOLASI SALMONELLA SP. DAN PREVALENSINYA PADA TEMBOLOK (INGLUVIENS) AYAM
BURAS DAN AYAM RAS DI PASAR AYAM PEUNAYONG KOTA BANDA ACEH (Isolation of
Salmonella sp. and Prevalence from Crops (Ingluviens) in Local Chicken and
Broiler in Poultry Market Peunayong Banda Aceh)
- Authors: Sri wahyuni, Sri Wahyuni
  Pages: 314 - 322
  Abstract: Abstract The crop is the part of the chicken's digestive tract that is more likely to break during processing. This research aimed to isolate Salmonella sp. from crop in local chickens and broiler from the Peunayong Chicken Market in Banda Aceh. Samples were swab contents of crop from 15 crops of local chickens and 15 crops of broiler. This research is a Cross sectional study with Carter method, starts with a swab of crop contents and then is inserted into the Selenite Cystine Broth (SCB) media, if the color of the media becomes orange, then followed by streaking bacteria on Salmonella Shigella Agar (SSA) media. Then observed the morphology that grew on SSA media including size, shape, surface, elevation, edges, aspects and colony color, followed by Gram stain to observe bacterial morphology microscopically. The research data were analyzed descriptively, displayed in figures and tables. The results of the isolation showed that 7 from 15 samples (46%) of local chicken cropping and 15 from 15 samples (100%) of broiler were positive for Salmonella sp. Therefore, it can be concluded that Salmonella sp. can be isolated from local chicken and broiler, Salmonella sp. prevalence is higher in broiler than in local chicken.Keywords : Eviserasi; Food borne disease; Gram negatif; Zoonotic disease Abstrak Tembolok merupakan bagian saluran pencernaan ayam yang lebih mungkin pecah selama pemrosesan. Penelitian ini bertujuan untuk mengisolasi Salmonella sp. pada tembolok ayam buras dan ayam ras yang berasal dari Pasar Ayam Peunayong Kota Banda Aceh. Sampel berupa swab isi tembolok dari 15 tembolok ayam buras dan 15 tembolok ayam ras. Penelitian ini merupakan Cross sectional dengan metode Carter dimulai dari swab isi tembolok kemudian dimasukkan kedalam media Selenite Cystine Broth (SCB), apabila warna media menjadi orange dilanjutkan dengan penanaman bakteri pada media Salmonella Shigella Agar (SSA). Kemudian diamati morfologi yang tumbuh pada media SSA meliputi ukuran, bentuk, permukaan, elevasi, tepi, aspek dan warna koloni, dilanjutkan dengan pewarnaan Gram untuk mengamati morfologi bakteri secara mikroskopis. Data hasil penelitian dianalisis secara deskriptif, ditampilkan dalam bentuk gambar dan tabel. Hasil isolasi didapatkan bahwa pada 7 dari 15 (46%) sampel tembolok ayam buras dan 15 dari 15 sampel (100%) sampel tembolok ayam ras positif terdapat Salmonella sp. Oleh sebab itu, dapat disimpulkan bahwa Salmonella sp. dapat disolasi dari tembolok ayam buras dan ayam ras, prevalensi Salmonella sp. lebih tinggi pada ayam ras dibandingkan ayam buras.Kata kunci : Eviserasi; Food borne Disease; Gram negative; Zoonotic disease
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.64584
  Issue No: Vol. 40, No. 3 (2022)
Kadar Hormon Prolaktin Pada Kambing Peranakan Ettawa,Sapera dan Saanen
Pada Berbagai Status Fisiologi
- Authors: Sarmin Sarmin Sarmin
  Pages: 323 - 327
  Abstract: This study examines the prolactin hormone profile in Etaawa, Sapera and Saanen lactating goats, adult females, pregnant does, and yearlings. This study used a total of 80 randomly selected goats with details of 26 Ettawa Crossbreed goats (eight lactating, six adult females, six pregnant, and six heifers), 32 Sapera goats (eight lactating, six adult females, twelve pregnant, and six yearlings) and 22 Saanen goats (five lactating, four adult females, six pregnant and yearling). All goats were declared clinically healthy. Blood sampling was performed through the jugular vein in each animal; then, serum was analyzed with an Enzyme-linked immunosorbent assay (ELISA) to prolactin concentration. High prolactin concentrations were found in Saanen goats in pregnant physiological status compared to Sapera and Ettawa crossbreed goats.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.76207
  Issue No: Vol. 40, No. 3 (2022)
Effect of Purslane (Portulaca oleracea L.) Ethanol Extract in
Alloxan-induced Hyperglycemic Rats (Rattus novergicus L.) Blood Glucose
Levels
- Authors: Anastasia Sylvianka Dwi Jayanti, Agung Janika Sitasiwi, Sri Isdadiyanto
  Pages: 325 - 331
  Abstract: Diabetes mellitus (DM) is a chronic condition which caused by insulin secretion disorder, insulin resistance or both. High number of diabetics per year has led many researchers to conduct research to find the most safe and effective medicine for DM treatment. Flavonoid are known to act as inhibitors of α-amylase as a parameter of pancreatic damage and help lower blood glucose levels. One source of flavonoids is purslane. The aim of this study was to examine the effect of purslane ethanol extract on glucose and α-amylase levels in rat. Twenty rats Rattus novergicus L. strain Wistar are divided into 5 groups; control, positive control, and three test groups, each was given purslane ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 300 mg/kgBW. Blood glucose and body weight were measured before alloxan induction, after induction, as well as day- 7, 14, 21, and 28; while feed and drink consumption were measured everyday starting with the administration of purslane ethanol extract. The α-amylase test was carried out on the 28th day using enzymatic UV determination of degradation product of maltose and glucose method. The analysis result showed that rats blood glucose and α-amylase decreased in each group with purslane extract. The antioxidant content of flavonoids in purslane ethanol extract has been shown to have anti-diabetic effects by repairing pancreatic function.
  PubDate: 2022-12-12
  DOI: 10.22146/jsv.61937
  Issue No: Vol. 40, No. 3 (2022)
Aktivitas Ekstrak Rimpang Kunyit (Curcuma longa L) pada Mencit Parkinson
yang Diinduksi Haloperidol
- Authors: Sapto Yuliani, Mochammad Saiful Bachri, Vivi Sofia, Wahyu Widyaningsih, Dandy Annas Muttaqien, Galuh Rista Putri, Nadia Selvia, Sofina Rahmadita, Intan Dwi Rahmita
  Pages: 329 - 336
  Abstract: Penyakit Parkinson merupakan penyakit neurodegeneratif dengan gangguan motorik seperti bradikinesia, rigiditas, dan tremor yang dapat mempengaruhi keseimbangan tubuh. Hal ini terjadi karena penurunan kadar dopamin yang dapat dipicu oleh adanya stres oksidatif. Kunyit (Curcuma longa) mengandung kurkumin yang mempunyai aktivitas antioksidatif dan neuroprotektif yang potensial sehingga dapat mencegah gangguan neurodegeneratif karena stres oksidatif. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak rimpang kunyit pada mencit model Parkinson yang diinduksi haloperidol. Ekstraksi rimpang kunyit dilakukan secara maserasi dengan pelarut etanol 96%. Sebanyak 60 ekor mencit dibagi menjadi 6 kelompok, masing-masing mendapat perlakukan sebagai berikut : Kelompok normal diberi CMC-Na per oral (p.o) dan NaCl fisiologis intraperitoneal (i.p), kelompok haloperidol diberi CMC -Na (p.o) dan larutan haloperidol i.p (1 mg/kg BB), kelompok levodopa diberi levodopa (p.o) dan larutan haloperidol i.p, Kelompok ekstrak 100 diberi ekstrak kunyit dosis 100 mg/kg BB dan larutan haloperidol i.p , Kelompok ekstrak 200 diberi ekstrak kunyit dosis 200 mg/kg BB dan larutan haloperidol i.p, Kelompok ekstrak 400 diberi ekstrak kunyit dosis 400 mg/kg BB dan larutan haloperidol i.p. Pemberian CMC-Na, levodopa dan ekstrak kunyit diberikan selama 21 hari. Lima belas menit setelah pemberian perlakuan hari terakhir, semua hewan uji kecuali kelompok 1 diinjeksi haloperidol dosis 1 mg/kg BB secara i.p. Kemudian dilakukan uji batang, uji roda berputar, uji refleks geotaksis, uji refleks menghindari jurang dan uji kemampuan penciuman pada menit ke 5, 60, 120 dan 180. Data yang diperoleh dianalisis menggunakan uji anova satu jalur dilanjutkan dengan uji post hoc LSD. Hasil analisis data menunjukkan bahwa pemberian haloperidol dapat meningkatkan waktu katalepsi, meningkatkan jumlah jatuh dan mempercepat waktu jatuh pertama kali, menurunkan refleks geotaksis, meningkatkan waktu refleks menghindari jurang serta menurunkan kemampuan indera penciuman. Sedangkan pemberian ekstrak kunyit dosis 200 dan 400 mg/kgBB dapat menurunkan waktu katalepsi, menurunkan jumlah jatuh dan memperlama waktu jatuh pertama kali, menurunkan refleks geotaksis, menurunkan lama waktu pada uji refleks menghindari jurang serta meningkatkan kemampuan indera penciuman jika dibandingkan dengan Haloperidol (P<0.05). Pemberian ekstrak 200 dan 400 mg/kg tidak berbeda bermakna (P>0,05) dengan pemberian levodopa. Berdasarkan hasil dapat disimpulkan bahwa ekstrak rimpang kunyit dosis 200 dan 400 mg/kgBB memiliki aktivitas untuk mencegah terjadinya Parkinson pada mencit yang diberi haloperidol.
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.71871
  Issue No: Vol. 40, No. 3 (2022)
TEPUNG BEKICOT (Achatina fulica) SEBAGAI PAKAN TAMBAHAN UNTUK MENINGKATKAN
KUALITAS SEMEN DOMBA (Ovis aries)
- Authors: Jayanti Berliana Dewi
  Pages: 337 - 342
  Abstract: One of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increase
  PubDate: 2022-12-01
  DOI: 10.22146/jsv.69114
  Issue No: Vol. 40, No. 3 (2022)