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  Subjects -> PHARMACY AND PHARMACOLOGY (Total: 575 journals)
Showing 401 - 253 of 253 Journals sorted by number of followers
Pharmazeutische Zeitung     Full-text available via subscription   (Followers: 11)
Pharmazeutische Industrie     Full-text available via subscription   (Followers: 9)
Pharmaceutical Journal     Free   (Followers: 8)
Advanced Herbal Medicine     Open Access   (Followers: 8)
Pharmaceutical Fronts     Open Access   (Followers: 7)
Molekul     Open Access   (Followers: 7)
Advances in Pharmacology and Pharmacy     Open Access   (Followers: 6)
Drug Metabolism and Pharmacokinetics     Hybrid Journal   (Followers: 6)
AAPS Open     Open Access   (Followers: 5)
Journal of Drug Research in Ayurvedic Sciences     Open Access   (Followers: 4)
ACS Pharmacology & Translational Science     Hybrid Journal   (Followers: 4)
Advances in Traditional Medicine     Hybrid Journal   (Followers: 3)
Canadian Journal of Pain     Open Access   (Followers: 3)
PharmaNutrition     Hybrid Journal   (Followers: 3)
Journal of Drug Delivery Science and Technology     Hybrid Journal   (Followers: 3)
Journal of Herbal Science     Full-text available via subscription   (Followers: 3)
Journal of Pharmacological Sciences     Open Access   (Followers: 3)
Indian Journal of Drugs in Dermatology     Open Access   (Followers: 2)
British Journal of Pharmacy (BJPharm)     Open Access   (Followers: 2)
Current Research in Drug Discovery     Open Access   (Followers: 2)
Journal of Herbal Medicine     Hybrid Journal   (Followers: 2)
Asian Journal of Pharmaceutical Research and Health Care     Open Access   (Followers: 2)
Pharmacological Research - Modern Chinese Medicine     Open Access   (Followers: 2)
Archives of Pharmacy and Pharmaceutical Sciences     Open Access   (Followers: 2)
Current Issues in Pharmacy and Medical Sciences     Open Access   (Followers: 2)
International Journal of Immunopathology and Pharmacology     Open Access   (Followers: 2)
Advances in Pharmacoepidemiology & Drug Safety     Open Access   (Followers: 2)
European Journal of Medicinal Plants     Open Access   (Followers: 2)
Journal of Pharmaceutical Sciences and Pharmacology     Full-text available via subscription   (Followers: 2)
Journal of Education and Science     Open Access   (Followers: 2)
Drug Safety - Case Reports     Open Access   (Followers: 2)
Current Research in Pharmacology and Drug Discovery     Open Access   (Followers: 1)
Advances in Medical, Pharmaceutical and Dental Research     Open Access   (Followers: 1)
Pediatric Pharmacology     Open Access   (Followers: 1)
Journal of Metabolomics & Systems Biology     Open Access   (Followers: 1)
Regulatory Mechanisms in Biosystems     Open Access   (Followers: 1)
Integrative Medicine International     Open Access   (Followers: 1)
Journal of Applied Pharmaceutical Research     Open Access   (Followers: 1)
Herbal Medicines Journal     Open Access   (Followers: 1)
Farmakoèkonomika : Modern Pharmacoeconomic and Pharmacoepidemiology     Open Access   (Followers: 1)
Separation Science plus (SSC plus)     Hybrid Journal   (Followers: 1)
Advanced Therapeutics     Hybrid Journal   (Followers: 1)
Journal of Biopharmaceutics Sciences     Open Access   (Followers: 1)
Expert Review of Precision Medicine and Drug Development     Hybrid Journal   (Followers: 1)
Psychiatry and Clinical Psychopharmacology     Open Access   (Followers: 1)
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian     Open Access   (Followers: 1)
Emerging Trends in Drugs, Addictions, and Health     Open Access   (Followers: 1)
Journal of Medicinal Plants for Economic Development     Open Access   (Followers: 1)
OpenNano     Open Access   (Followers: 1)
Safety and Risk of Pharmacotherapy     Open Access   (Followers: 1)
Journal of Pharmaceutical Technology, Research and Management     Open Access   (Followers: 1)
Journal of the American College of Clinical Pharmacy : JACCP     Hybrid Journal   (Followers: 1)
Open Pharmacoeconomics & Health Economics Journal     Open Access   (Followers: 1)
Pharmactuel     Open Access   (Followers: 1)
Al-Azhar Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Chinese Herbal Medicines     Full-text available via subscription   (Followers: 1)
Drugs : Real World Outcomes     Hybrid Journal   (Followers: 1)
Pharmakon : Arzneimittel in Wissenschaft und Praxis     Full-text available via subscription   (Followers: 1)
Sustainable Chemistry and Pharmacy     Full-text available via subscription   (Followers: 1)
Medicines     Open Access   (Followers: 1)
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 1)
Future Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Journal of Pharmaceutical Health Care and Sciences     Open Access   (Followers: 1)
East and Central African Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Ethiopian Pharmaceutical Journal     Full-text available via subscription   (Followers: 1)
Pharmacy & Pharmacology     Open Access   (Followers: 1)
Clinical Complementary Medicine and Pharmacology     Open Access  
Exploratory Research in Clinical and Social Pharmacy     Open Access  
Indonesian Journal of Pharmaceutical Education     Open Access  
Future Drug Discovery     Open Access  
IUPHAR/BPS Guide to Pharmacology CITE     Open Access  
Journal of Applied Pharmaceutical Sciences and Research     Open Access  
Cephalalgia Reports     Open Access  
Pharmacon : Jurnal Farmasi Indonesia     Open Access  
Pharmacia     Open Access  
Research Results in Pharmacology     Open Access  
Journal of Toxins     Open Access  
Journal of Pharmaceutics & Pharmacology     Open Access  
Natural Product Communications     Open Access  
PharmaTutor     Open Access  
International Journal of Pharmaceutical Sciences and Developmental Research     Open Access  
Toxicological Research     Hybrid Journal  
Current Medical Science     Hybrid Journal  
EUREKA : Health Sciences     Open Access  
Iraqi Journal of Pharmacy     Open Access  
Revista Colombiana de Ciencias Químico-Farmacéuticas     Open Access  
Medicine in Drug Discovery     Open Access  
Frontiers in Medical Technology     Open Access  
International Journal of Medical and Pharmaceutical Case Reports     Open Access  
Asian Journal of Research in Medical and Pharmaceutical Sciences     Open Access  
Journal of Pharmaceutical Health Services Research     Hybrid Journal  
AboutOpen     Open Access  
Current Protocols in Pharmacology     Hybrid Journal  
Medical Cannabis and Cannabinoids     Open Access  
Ukrainian Biopharmaceutical Journal     Open Access  
Pharmaceutical Journal of Sri Lanka     Open Access  
Isan Journal of Pharmaceutical Sciences (IJPS)     Open Access  
Ciencia e Investigación     Open Access  
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)     Open Access  
Toxicon : X     Open Access  
International Journal of Pharmaceutics: X     Open Access  
Jurnal Farmasi dan Ilmu Kefarmasian Indonesia     Open Access  
Open Pharmacology Journal     Open Access  
ExRNA     Open Access  
International Journal of Pharmaceutics & Pharmacology     Open Access  
Antibody Therapeutics     Open Access  
Journal of Faculty of Pharmacy of Ankara University     Open Access  
Iraqi Journal of Pharmaceutical Sciences     Open Access  
Journal of Medicinal Botany     Open Access  
Journal of Medicinal Herbs and Ethnomedicine     Open Access  
International Journal of Pharmacokinetics     Hybrid Journal  
Neuropsychopharmacology Reports     Open Access  
Reviews on Clinical Pharmacology and Drug Therapy     Full-text available via subscription  
SynOpen     Open Access  
Matrix Science Pharma     Open Access  
Contract Pharma     Full-text available via subscription  
Journal of Cellular Neuroscience and Oxidative Stress     Open Access  
Istanbul Journal of Pharmacy     Open Access  
Acta Pharmaceutica Indonesia     Open Access  
Indonesian Journal of Pharmacy     Open Access  
EMC - Cosmetologia Medica e Medicina degli Inestetismi Cutanei     Full-text available via subscription  
High-Throughput     Open Access  
Scientia Pharmaceutica     Open Access  
Trends in Peptide and Protein Sciences     Open Access  
Acta Physiologica Hungarica     Full-text available via subscription  
Jurnal Farmasi Sains dan Praktis     Open Access  
Jurnal Kefarmasian Indonesia     Open Access  
Pharmaceutical Historian     Open Access  
Planta Medica International Open     Open Access  
Archives of Razi Institute     Open Access  
Journal of Pharmaceutical Research     Open Access  
EJNMMI Radiopharmacy and Chemistry     Open Access  
FarmaJournal     Open Access  
Journal of Negative and No Positive Results     Open Access  
Pharmaciana     Open Access  
Folia Medica Indonesiana     Open Access  
Актуальні питання фармацевтичної та медичної науки та практики     Open Access  
International Journal of Pharmacology, Phytochemistry and Ethnomedicine     Open Access  
Фармацевтичний часопис     Open Access  
Ciência Equatorial     Open Access  
Iranian Journal of Pharmaceutical Research     Open Access  
Research & Reviews : A Journal of Drug Design & Discovery     Full-text available via subscription  
Asian Journal of Medical and Pharmaceutical Researches     Open Access  
Research & Reviews : A Journal of Pharmacognosy     Full-text available via subscription  
Ars Pharmaceutica     Open Access  
Pharmacognosy Communications     Partially Free  
Egyptian Pharmaceutical Journal     Open Access  
Pharmacological Reports     Hybrid Journal  
PZ Prisma : Materialien zur Fort- und Weiterbildung     Full-text available via subscription  
Chinese Journal of Pharmaceutical Analysis     Full-text available via subscription  
Journal of Nanopharmaceutics and Drug Delivery     Full-text available via subscription  
Journal of Hydrogels     Full-text available via subscription  
Manufacturing Chemist     Full-text available via subscription  
Pharmaceutica Analytica Acta     Open Access  
Journal of Pharmacovigilance     Open Access  
Current Pharmacology Reports     Hybrid Journal  
Nigerian Journal of Natural Products and Medicine     Full-text available via subscription  
International Journal of Herbs and Pharmacological Research     Open Access  
Bangladesh Journal of Physiology and Pharmacology     Open Access  
Research Journal of Pharmacognosy     Open Access  

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Ukrainian Biopharmaceutical Journal
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2311-715X - ISSN (Online) 2519-8750
Published by National University of Pharmacy Homepage  [1 journal]
  • The choice of excipients to obtain tablets based on the Primula
           denticulate Smith. Extract by wet granulation

    • Authors: A. Yu. Shyrko, M.M. Vasenda, L.I. Будняк, O.O. Pokotylo
      Pages: 4 - 9
      Abstract: Introduction. Herbal medicines are increasingly being used in the treatment of various diseases. Significant number of resources, high-level availability and the possibility of cultivation have provided high prospects of plant raw materials in the development of new herbal medicines. Primula denticulate Smith. is one of the most interesting medicinal plant raw materials source in modern medicine and pharmacy with the wide spectrum of pharmacotherapeutic action, that is mainly used only in traditional medicine. Thus, nowadays the development of new and effective medicines based on Primula denticulate Smith. in tablet dosage form is the topical task of pharmacy. The aim of the work. To study the influence of excipients on the pharmaco­technological parameters of tablets with Primula denticulate Smith, are obtained by wet granulation method and to substantiate the choosing of the best excipients by the method of mathematical planning of the experiment. Materials and Methods. It was used a self-prepared dense extract of Primula denticulate Smith. and excipients, which are complied with the requirements of State Pharmacopoeia of Ukraine in terms of pharmaco-technological quality parameters, for developing tablets. The hyper Greek-Latin square 4x4 was used to study the four qualitative factors. Results and Discussion. In the course of the experiment, it was determined the dependence of pharmaco­technological parameters (friability, resistance to crushing and disintegration of tablets) on type of excipients. It was selected excipients with the optimal pharmaco-technological parameters for further research the tablets quality. Conclusions. According to the method of mathematical planning of the experiment, it’s chosen the optimal excipients for obtaining tablets based on dense extract of Primula denticulate Smith. Considering the investigated pharmaco-technological properties (friability, resistance to crushing and disintegration of tablets) the qualitative composition of excipients for obtaining tablets by the method of wet granulation was determined: Avicel PH - 105 and MCC 101, croscarmellose sodium, colloidal anhydrous silicon dioxide, magnesium stearate and Prosolv 90.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.296
       
  • The substantiation of the choice of a gel former for developing the gel
           composition with hyaluronic acid and decametoxin

    • Authors: G. Melnyik, T. Yarnykh, M. Buryak
      Pages: 10 - 14
      Abstract: Topicality. Bedsores are an urgent problem of modern medicine both at the outpatient and inpatient stage. For the treatment of infected bedsores it is advisable to use drugs that have a complex effect focused on various parts of the pathological process. For the treatment of chronic wounds and bedsores together with the inhibition of the growth of microorganisms the drug must provide the optimal conditions for the growth of granulation, have a reparative and anti-inflammatory effect. Aim. To experimentally substantiate the composition of the gel base with hyaluronic acid and decamethoxine. Materials and methods. To select the structure-forming component in the gel base, the possibility of using “Ultrez 10 NF” carbomer, “Aristoflex AVC” (copolymer of acrylamidomethylpropanesulfonic acid and vinyl pyrrolidone) from Clariant Surfactants company, “Structure XL” (hydropropylated corn starch) as a gelling agent was studied. Organoleptic, physicochemical, structural and mechanical parameters of gel model samples were determined according to the methods of the State Pharmacopoeia of Ukraine. The statistical processing of the results was performed using the Statistica 6.0 program. Results and discussion. The choice of the optimal gelling agent was performed taking into account the results of the study of organoleptic, physicochemical, structural and mechanical parameters of gel model samples. The experimental studies conducted allowed to choose the optimal gelling agent – “Aristoflex AVC” in the concentration of 1 %. It is easily dispersed with water and does not require the addition of a neutralizer. It has been proven that the base selected has the optimal consumer, structural-mechanical, and physicochemical properties. Conclusions. Based on the organoleptic, physicochemical, structural-mechanical studies the composition of the gel base with hyaluronic acid and decamethoxine has been experimentally substantiated.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.298
       
  • The study of the cytotoxic activity of the dry extract and the anthocyanin
           complex of Gebu Dahlia variety flowers

    • Authors: L. Maloshtan, L. Shakina, T. Gontova, S. Romanova, M. Yaremenko
      Pages: 16 - 22
      Abstract: Topicality. For today, the search for promising plants with a high content of anthocyanins to create safe herbal medicines is an urgent problem of modern science. Aim. To study the presence and severity of the cytotoxic activity of the dry extract of Gebu Dahlia variety flowers, the qualitative analysis and quantify the amount of anthocyanins in flowers of this variety. Materials and methods. For the study, the dry extract of Gebu flowers was obtained. The cytotoxicity analysis of 1 %; 0.5 %; 0.25 %; 0.125 %; 0.0625 % solutions of the extract were performed by microscopy on red bone marrow (RBM) cells of rats at an exposure of 15, 45, 90 minutes. The spectrophotometric study and high-performance liquid chromatography were used to assess the quantitative and qualitative content of the amount of anthocyanins. Results and discussion. The results obtained indicate that the viability of RBM cells is affected by the concentration of the extract and exposure: in the concentrations of 0.063-0.125 % no toxic effect of the extract was detected, while 0.25-1 % solutions were able to show cytostatic properties. The content of anthocyanin amount in the flowers was determined (1.8 % ± 0.02); 18 substances were identified, among them substances from the groups of cyanidin (54.7 %) and delphinidin (28 %) predominated. Conclusions. The first stage of testing of the dry extract from Gebu Dahlia variety flowers for potential toxicity has been carried out: the extract is potentially non-toxic in the lowest of the concentrations studied. For the first time, the content of anthocyanins in flowers of Gebu Dahlia variety has been determined, 18 substances from the groups of delphinidin, cyanidin, petunidine, peonidine, malvidin have been identified.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.295
       
  • The study of the antioxidant activity of polyphenol extracts from
           cranberry leaves on the model of insulin resistance

    • Authors: O. Krasilnikova, G. Kravchenko, O. Shovkova
      Pages: 23 - 27
      Abstract: Topicality. In Ukraine, the number of patients with diabetes is growing rapidly, it ranks the 4th in the structure of diseases of the endocrine system, and it is one of the main causes of incapacitation and disability among the population and takes the second place by the number of deaths. Polyphenolic extracts from leaves of the heather (Ericaceae) family, in particular blueberries, cranberries and bearberry, in the previous studies demonstrated the presence of antioxidant, hypoglycemic and lipotropic properties. However, the pharmacological activity of extracts from cranberry leaves has not been sufficiently studied. Aim. To study the effect of polyphenolic extracts from cranberry leaves on the processes of LPO and the state of the antioxidant defense in the liver and serum in rats with the experimental insulin resistance. Materials and methods. The study was performed on white outbred male rats weighing 180 ± 15 g, kept in the vivarium of the NUPh. Insulin resistance was induced by keeping animals on a high fructose diet for 6 weeks according to the recommendations. The extracts were administered intragastrically in the dose of 200 μg/100 g of the body weight from the fourth week of the experiment for 14 days. The POL activity was assessed by the level of TBA reactants, diene conjugates and conjugated trienes in the liver and serum. The state of the antioxidant defense was assessed by the level of reduced glutathione, as well as the activity of catalase and superoxide dismutase. Indicators were determined by the spectrophotometric method. Results and discussion. The development of the experimental resistance was accompanied by an increase in LPS activity in the liver and serum of rats. In the serum of animals with IR the content of primary products of LPO increased in 1.75 times, the content of triene conjugates in 1.7 times, respectively, while the content of TBA reactants in 1.61 times. The introduction of the polyphenolic concentrate from cranberry leaves was accompanied by a sharp decrease in the products of lipid peroxidation both in the eserum and liver to almost ascending levels. There was normalization of the activity of enzymes of the first line of the antioxidant defense compared to animals with insulin resistance due to the presence of plant flavonoids in the extract. Conclusions. Thus, the data obtained indicate that the polyphenolic extract from cranberry leaves exhibits antioxidant properties in the liver and serum of rats with the experimental insulin resistance. The polyphenolic extract also restored the antioxidant defense of cells and the body as a whole. The data obtained indicate the need for further research to create a new herbal medicine for use in the treatment of insulin resistance and diabetes.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.297
       
  • The study of the antidepressant and actoprotective activity of a synthetic
           analog of the terminal region of neuropeptide Y

    • Authors: I. O. Havrylov, S. Yu. Shtrygol’
      Pages: 28 - 35
      Abstract: Topicality. Neuropeptide Y (NPY), which is significantly widespread in the human body, is responsible for various physiological processes, in particular it is involved in the regulation of the emotional behavior, memory and metabolism. The modified short fragment of NPY proposed – NP9 nonapeptide for intranasal administration – shows certain biological properties of the native peptide, in particular the anxiolytic and antiamnesic action. The effect of NP9 on affective disorders of depressive nature and physical endurance remains unknown. Aim. To study the antidepressant and actoprotective activity of NP9 in models of tail suspension, reserpine-induced depression, and forced weighted swimming. Materials and methods. The antidepressant properties of NP9 were studied in the immobilization tail suspension test in mice and on a model of reserpine-induced depression with registration of the body temperature, ptosis, and behavior in the Porsolt forced swimming tests and the open field test. The actoprotective properties of NP9 were studied in the swimming test with a load (10 % of the weight of the animal). Results and discussion. In the tail suspension test, NP9 significantly increased the latent time of the first immobilization compared to the indicators of intact animals (3.2 times) and the imipramine group (2.7 times). NP9 did not significantly affect the manifestations of reserpine-induced depression. These results indicate the weak antidepressant properties of NP9. Nonapeptide significantly increased the swimming time to exhaustion by 2.5 times, indicating actoprotective properties, according to which NP9 exceeded the reference drug Semax. Conclusions. In the tail suspension test, nonapeptide NP9 exhibits dose-dependent antidepressant properties after the first administration, but this effect is practically not manifested in the rigid reserpine-induced model of depression. NP9 increases the physical endurance of animals in the forced weighted swimming test and is superior to the reference drug Semax by its effectiveness.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.301
       
  • A comparative study of the hepatotrophic properties of non-steroidal
           anti-inflammatory drugs

    • Authors: К. Г. Щекіна, G. Belik, D. Semeniv, V. Ulanova
      Pages: 36 - 41
      Abstract: Topicality. Recently, more and more works on the hepatotoxicity of NSAIDs have appeared in the literature. Presumably, all NSAIDs have hepatotoxicity, but the degree of adverse effects on the liver in different drugs is variable. Aim. To study the effect of different generations of NSAIDs on the functional state of the liver of the experimental animals. Materials and methods. Diclofenac sodium, piroxicam, indomethacin, meloxicam and celecocosib were selected for the comparative study. Changes in the functional state of the liver of intact rats during subchronic administration of the drugs selected, as well as their impact on the course of the model hepatitis were determined. The state of the liver was determined by the following indicators: the mass coefficient of the liver, the activity of alanine aminotransferase, alkaline phosphatase, the content of total protein, urea, cholesterol in the blood serum, the level of TBA-active products, diene conjugates, reduced glutathione, catalase and glycogen in the liver homogenate. Results and discussion. It was found that diclofenac, piroxicam, indomethacin in the doses of ED50 by the antiexudative activity when used for 14 days adversely affected the liver of intact animals, as well as worsened the course of the model hepatitis, i.e. had a pronounced hepatotoxic effect. Meloxicam and celecoxib did not show a pronounced adverse effect in the carbon tetrachloride hepatitis, but contributed to the deterioration of the functional state of the liver of intact rats, i.e. had a moderate hepatotoxic effect. Conclusions. By the level of hepatotoxicity the drugs studied can be arranged as follows: diclofenac > indomethacin > piroxicam > meloxicam > celecoxib.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.303
       
  • Dynamics of connective tissue metabolites in the blood of rats of
           different age under hypokinesia

    • Authors: D. Morozenko, F. Leontieva, K. Gliebova
      Pages: 42 - 45
      Abstract: Topicality. Hypokinesia is a condition of insufficient motor activity of the body with limited pace and volume of movement, and is currently the fourth leading cause of endemic death in the world. It is known that hypokinesia can affect the metabolism of components of bone and cartilage, which is the basis for the development of pathological processes, but there are no biochemical markers of this process in the literature. Thus, currently, there is insufficient number of experimental scientific works devoted to the study of biochemical parameters characterizing the state of the connective tissue in the experiment to assess the impact of hypokinesia on the body, and this fact determines the relevance of the study. Aim. To study the dynamics of the main metabolites characterizing the state of the connective tissue in the blood serum in hypokinesia in rats of different ages. Materials and methods. The studies were performed on 42 white male rats aged 3 and 12 months, kept in the vivarium of the Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine, Kharkiv. The conditions of hypokinesia were reproduced using a specially designed device that limited the mobility of the animal without disturbing the ventilation of the body. Animals were removed from the experiment by decapitation in Day 7 and 30 of immobilization under thiopental anesthesia. The content of sialic acids in the serum was determined by the Hess method, chondroitin sulfate – by Nemeth-Csoka method modified by L. I. Slutsky, the fractional composition of glycosaminoglycans (GAG) – by the reaction with resoquine. Results and discussion. In the study of the rat serum, it was found that in intact animals of 3 and 12 months of age the content of sialic acids was different; it was 2.94 ± 0.16 and 1.98 ± 0.09 mmol/L, respectively. After 7 days of hypokinesia, their concentration in young animals did not change, but in older animals, this figure increased. By Day 30, the serum sialic acid levels suddenly increased compared to intact animals. In animals of 12 months of age, the concentration of sialic acids in the blood serum increased by both Day 7 and Day 30 of hypokinesia, especially at the end of the experiment. Hypokinesia also showed a significant increase in serum concentrations of chondroitin sulfates on Day 7 of the experiment in animals of both age groups. At the same time, the level of these metabolites on Day 30 was at the same level. In young rats on Day 7 the fraction of GAG remained unchanged, but on Day 30 there was a significant decrease. During this period, the second fraction of GAG increased. In animals aged 12 months on Day 7 and 30, the content of most GAG fractions was lower than the values characterizing the fractional composition of GAG in intact rats. Conclusions. Thus, the determination of sialic acids, GAG fractions and chondroitin sulfates in the serum can be presented as a set of biochemical tests to assess disorders of the connective tissue metabolism while limiting the motor activity of the body. It has been found that the 30-day hypokinesia causes significant disorders of the connective tissue, and it is reflected in changes in the biochemical parameters of the serum of experimental rats.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.304
       
  • Determination of clemastine by the HPLC method in the blood

    • Authors: V.I. Kabachny, О. Mamina , N. Bondarenko , O. Mozgova
      Pages: 54 - 61
      Abstract: Topicality. Сlemastine fumarate (tavegil)-1-methyl-2 [2-α-methyl-p-chlorobenzhydryloxy)-ethyl]-pyrrolidine fumarate is the first generation H1-histamine receptor blocker. Сlemastine fumarate selectively inhibits histamine H1 receptors and reduces capillary permeability. The drug has a pronounced anti-allergic and antipruritic effect. Clemastine prevents the development of vasodilation and the smooth muscle contraction induced by histamine. Сlemastine fumarate has an isignificant anticholinergic activity, causes sedation. The drug is used to treat pruritus in psoriasis, multiple sclerosis and optic neuritis. Clemastine is characterized by the following side effects: increased fatigue, drowsiness, sedation, weakness, lethargy, impaired coordination of movements; nausea, vomiting, decreased blood pressure, palpitations, hemolytic anemia, skin rash, anaphylactic shock. In case of an overdose, the drug has a neurotoxic effect, which manifests itself in impaired consciousness with the development of generalized anticholinergic convulsive syndrome. The urgent task for monitoring the treatment effectiveness of the population with сlemastine fumarate and diagnosis of drug intoxication is the choice of highly sensitive and selective research methods of its analysis in pharmaceuticals and biological matrices during the treatment. Aim. To develop an algorithm for directed analysis of clemastine in biological extracts from the blood using a unified method of the HPLC research. Materials and methods. The extraction of clemastine was performed with chloroform at Ph 9.0. The extracts were purified from impurities by a combination of TLC and extraction with hexane. The TLC purification and identification of clemastine were carried out under optimal conditions: the system of organic solvents – methanol – 25 % solution of ammonium hydroxide (100 : 1.5) and chromatographic plates – Sorbfil PTLC-AF-A, Rf сlemastine = 0.60 ± 0.03. To detect clemastine, the most sensitive location reagents were used –UV light (λ = 254 nm) and Dragendorff’s reagent modified by Mounier. The chromatographic analysis was performed on a “Milichrome A-02” microcolumn liquid chromatograph (EkoNova, Closed Joint-Stock Company, Russia) under standardized HPLC conditions: the reversed-phase variant using a metal column with a non-polar absorbent Prontosil 120-5C 18 AQ, 5 μm; the mobile phase in the linear gradient mode – from eluent А (5 % acetonitrile and 95 % buffer solution – 0.2 М solution of lithium perchlorate in 0.005 М solution of perchloric acid) to eluent B (100 % acetonitrile) for 40 min. Regeneration of the column was conducted for 2 min with the mixture of solvents; the flow rate of the mobile phase was 100 μl/min, the injection volume – 4 μl. The multichannel detection of the substance was performed using a two-beam multi-wave UV spectrophotometer at 8 wavelengths of 210, 220, 230, 240, 250, 260, 280, and 300 nm; the optimal value of the column temperature – 37-40 °С and the pump pressure – 2.8-3.2 MPa. Results and discussion. Isolation of clemastine from the blood was performed according to the method developed, including the extraction with chloroform at pH 9.0; the extraction purification of extracts with hexane from impurities; the TLC purification and identification of clemastine. Using the unified HPLC method clemastine was identified by retention parameters and spectral ratios. For the quantitative determination, a calibration graph or the straight line equation corresponding to this graph were used. The results obtained indicated the reliability and reproducibility of the method. It was found that the relative uncertainty of the average result in the analysis of clemastine in the blood was ε = ± 4.63 %, the relative standard deviation of the average result was RSDx = 1.67 %. Conclusions. Clemastine was extracted with chloroform at pH 9.0 from the blood. Purification of extracts from co-extractive compounds was performed by combining TLC and extraction with hexane. It has been found that when isolating сlemastine from the blood according to the methods developed it is possible to determine 36.05-39.55 % of ...
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.299
       
  • Prospects of creating a new medicine based on biologically active
           substances of bearberry leaves for correction of the metabolic syndrome

    • Authors: N. Chaika, O. Koshovyi, M. Mazen, A. Kravchenko, A. Kovalyova, I. Kireyev
      Pages: 54 - 61
      Abstract: Topicality. The metabolic syndrome is pathogenetically interrelated metabolic disorders in the condition of a sick person. A large set of factors is involved in its occurrence. Risk factors include genetic predisposition, overeating, insulin resistance, obesity, bad habits, hypodynamics, stress and unfavorable environmental conditions. First of all, defects of the lipid and carbohydrate metabolism provoke a cascade of genetic, metabolic, hormonal, nervous, inflammatory and other reactions and disorders in cells, tissues and organs, causing the metabolic syndrome and associated diseases, such as diabetes, kidney and gallstone disease, hypertension, platelet hyperaggregation, etc. Therefore, the rational use of synthetic and herbal medicines in the complex correction of these disorders can slow down the development of the metabolic syndrome. Aim. To develop the method for obtaining a dry modified extract from bearberry, study its chemical composition, hypoglycemic and hypolipidemic activity in order to determine the prospects of its use for the correction of the metabolic syndrome. Materials and methods. The study object was a dry extract of bearberry leaves modified with cysteine. HPLC and spectrophotometry were used to analyze the extract obtained. The hypoglycemic and hypolipidemic activity of dry extracts of bearberry was studied in rats with insulin resistance. Results and discussion. The method for obtaining a dry modified extract from bearberry leaves was developed by adding cysteine. Phenologlycoside (arbutin), 2 phenolic acids (gallic and ellagic), 6 flavonoids, 8 saponins were identified in the extract, and their quantitative content was determined. Hyperoside and catechin were dominant among flavonoids, and ursolic acid, uvaol, and lupeol prevailed among saponins. The content of the main groups of phenolic compounds was determined in the extract by spectrophotometry. The introduction of the dry extract from bearberry leaves modified with cysteine has a normalizing effect on metabolic disorders on the background of a high-fructose diet; therefore, it can be a promising agent for the correction of the metabolic syndrome. Conclusions. As a result of the research conducted, a new dry extract from bearberry leaves modified with cysteine has been created. The phytochemical composition, hypoglycemic and hypolipidemic activities of the extract have been studied, indicating the prospects for its use to correct the metabolic syndrome.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.305
       
  • Study of chlorophylls and carotenoids in Sansevieria hyacinthoides leaves

    • Authors: V. Velma, V. Kyslychenko, S. Velma, А. Popyk
      Pages: 62 - 65
      Abstract: Topicality. Sansevieria hyacinthoides (L.) Druce belongs to the Asparagaceae family. The plant is native to African countries. It has been introduced and naturalized in some countries of America, Asia, Australia and Europe. This species is grown as an ornamental plant in many countries, and as a medicinal plant in Africa. Aim. To determine the quantitative content of chlorophylls a and b and the amount of carotenoids in Sansevieria hyacinthoides leaves freshly collected and dried. Materials and methods. The quantitative content was determined by the spectrophotometric method. This method allows determining the amount of chlorophyll a and b and carotenoids by the characteristics of the absorption spectra without their prior separation. Results and discussion. The amount of chlorophyll a and b in the dried leaves was almost 7 times higher than in the freshly collected ones (1.82 ± 0.036 mg/g vs. 0.25 ± 0.005 mg/g, and 2.08 ± 0.038 mg/g vs. 0.29 ± 0.006 mg/g, respectively). The quantitative content of the amount of carotenoids differed by 3.6 times (0.05 ± 0.001 mg/g in freshly collected leaves, and 0.18 ± 0.004 mg/g in the dried raw material). Conclusions. The quantitative content of the amount of carotenoids, chlorophyll a and chlorophyll b in freshly collected and dried leaves of Sansevieria hyacinthoides has been determined by the spectrophotometric method. The dried plant raw material of Sansevieria leaves has a higher content of biologically active substances: chlorophyll a – 1.82 ± 0.036 mg/g, chlorophyll b – 2.08 ± 0.038 mg/g, and the amount of carotenoids – 0.18 ± 0.004 mg/g.
      PubDate: 2021-04-12
      DOI: 10.24959/ubphj.21.302
       
 
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