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  Subjects -> PHARMACY AND PHARMACOLOGY (Total: 575 journals)
Showing 401 - 253 of 253 Journals sorted by number of followers
Pharmazeutische Zeitung     Full-text available via subscription   (Followers: 11)
Pharmazeutische Industrie     Full-text available via subscription   (Followers: 9)
Pharmaceutical Journal     Free   (Followers: 8)
Advanced Herbal Medicine     Open Access   (Followers: 8)
Pharmaceutical Fronts     Open Access   (Followers: 7)
Molekul     Open Access   (Followers: 7)
Advances in Pharmacology and Pharmacy     Open Access   (Followers: 6)
Drug Metabolism and Pharmacokinetics     Hybrid Journal   (Followers: 6)
AAPS Open     Open Access   (Followers: 5)
Journal of Drug Research in Ayurvedic Sciences     Open Access   (Followers: 4)
ACS Pharmacology & Translational Science     Hybrid Journal   (Followers: 4)
Advances in Traditional Medicine     Hybrid Journal   (Followers: 3)
Canadian Journal of Pain     Open Access   (Followers: 3)
PharmaNutrition     Hybrid Journal   (Followers: 3)
Journal of Drug Delivery Science and Technology     Hybrid Journal   (Followers: 3)
Journal of Herbal Science     Full-text available via subscription   (Followers: 3)
Journal of Pharmacological Sciences     Open Access   (Followers: 3)
Indian Journal of Drugs in Dermatology     Open Access   (Followers: 2)
British Journal of Pharmacy (BJPharm)     Open Access   (Followers: 2)
Current Research in Drug Discovery     Open Access   (Followers: 2)
Journal of Herbal Medicine     Hybrid Journal   (Followers: 2)
Asian Journal of Pharmaceutical Research and Health Care     Open Access   (Followers: 2)
Pharmacological Research - Modern Chinese Medicine     Open Access   (Followers: 2)
Archives of Pharmacy and Pharmaceutical Sciences     Open Access   (Followers: 2)
Current Issues in Pharmacy and Medical Sciences     Open Access   (Followers: 2)
International Journal of Immunopathology and Pharmacology     Open Access   (Followers: 2)
Advances in Pharmacoepidemiology & Drug Safety     Open Access   (Followers: 2)
European Journal of Medicinal Plants     Open Access   (Followers: 2)
Journal of Pharmaceutical Sciences and Pharmacology     Full-text available via subscription   (Followers: 2)
Journal of Education and Science     Open Access   (Followers: 2)
Drug Safety - Case Reports     Open Access   (Followers: 2)
Current Research in Pharmacology and Drug Discovery     Open Access   (Followers: 1)
Advances in Medical, Pharmaceutical and Dental Research     Open Access   (Followers: 1)
Pediatric Pharmacology     Open Access   (Followers: 1)
Journal of Metabolomics & Systems Biology     Open Access   (Followers: 1)
Regulatory Mechanisms in Biosystems     Open Access   (Followers: 1)
Integrative Medicine International     Open Access   (Followers: 1)
Journal of Applied Pharmaceutical Research     Open Access   (Followers: 1)
Herbal Medicines Journal     Open Access   (Followers: 1)
Farmakoèkonomika : Modern Pharmacoeconomic and Pharmacoepidemiology     Open Access   (Followers: 1)
Separation Science plus (SSC plus)     Hybrid Journal   (Followers: 1)
Advanced Therapeutics     Hybrid Journal   (Followers: 1)
Journal of Biopharmaceutics Sciences     Open Access   (Followers: 1)
Expert Review of Precision Medicine and Drug Development     Hybrid Journal   (Followers: 1)
Psychiatry and Clinical Psychopharmacology     Open Access   (Followers: 1)
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian     Open Access   (Followers: 1)
Emerging Trends in Drugs, Addictions, and Health     Open Access   (Followers: 1)
Journal of Medicinal Plants for Economic Development     Open Access   (Followers: 1)
OpenNano     Open Access   (Followers: 1)
Safety and Risk of Pharmacotherapy     Open Access   (Followers: 1)
Journal of Pharmaceutical Technology, Research and Management     Open Access   (Followers: 1)
Journal of the American College of Clinical Pharmacy : JACCP     Hybrid Journal   (Followers: 1)
Open Pharmacoeconomics & Health Economics Journal     Open Access   (Followers: 1)
Pharmactuel     Open Access   (Followers: 1)
Al-Azhar Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Chinese Herbal Medicines     Full-text available via subscription   (Followers: 1)
Drugs : Real World Outcomes     Hybrid Journal   (Followers: 1)
Pharmakon : Arzneimittel in Wissenschaft und Praxis     Full-text available via subscription   (Followers: 1)
Sustainable Chemistry and Pharmacy     Full-text available via subscription   (Followers: 1)
Medicines     Open Access   (Followers: 1)
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 1)
Future Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Journal of Pharmaceutical Health Care and Sciences     Open Access   (Followers: 1)
East and Central African Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Ethiopian Pharmaceutical Journal     Full-text available via subscription   (Followers: 1)
Pharmacy & Pharmacology     Open Access   (Followers: 1)
Clinical Complementary Medicine and Pharmacology     Open Access  
Exploratory Research in Clinical and Social Pharmacy     Open Access  
Indonesian Journal of Pharmaceutical Education     Open Access  
Future Drug Discovery     Open Access  
IUPHAR/BPS Guide to Pharmacology CITE     Open Access  
Journal of Applied Pharmaceutical Sciences and Research     Open Access  
Cephalalgia Reports     Open Access  
Pharmacon : Jurnal Farmasi Indonesia     Open Access  
Pharmacia     Open Access  
Research Results in Pharmacology     Open Access  
Journal of Toxins     Open Access  
Journal of Pharmaceutics & Pharmacology     Open Access  
Natural Product Communications     Open Access  
PharmaTutor     Open Access  
International Journal of Pharmaceutical Sciences and Developmental Research     Open Access  
Toxicological Research     Hybrid Journal  
Current Medical Science     Hybrid Journal  
EUREKA : Health Sciences     Open Access  
Iraqi Journal of Pharmacy     Open Access  
Revista Colombiana de Ciencias Químico-Farmacéuticas     Open Access  
Medicine in Drug Discovery     Open Access  
Frontiers in Medical Technology     Open Access  
International Journal of Medical and Pharmaceutical Case Reports     Open Access  
Asian Journal of Research in Medical and Pharmaceutical Sciences     Open Access  
Journal of Pharmaceutical Health Services Research     Hybrid Journal  
AboutOpen     Open Access  
Current Protocols in Pharmacology     Hybrid Journal  
Medical Cannabis and Cannabinoids     Open Access  
Ukrainian Biopharmaceutical Journal     Open Access  
Pharmaceutical Journal of Sri Lanka     Open Access  
Isan Journal of Pharmaceutical Sciences (IJPS)     Open Access  
Ciencia e Investigación     Open Access  
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community)     Open Access  
Toxicon : X     Open Access  
International Journal of Pharmaceutics: X     Open Access  
Jurnal Farmasi dan Ilmu Kefarmasian Indonesia     Open Access  
Open Pharmacology Journal     Open Access  
ExRNA     Open Access  
International Journal of Pharmaceutics & Pharmacology     Open Access  
Antibody Therapeutics     Open Access  
Journal of Faculty of Pharmacy of Ankara University     Open Access  
Iraqi Journal of Pharmaceutical Sciences     Open Access  
Journal of Medicinal Botany     Open Access  
Journal of Medicinal Herbs and Ethnomedicine     Open Access  
International Journal of Pharmacokinetics     Hybrid Journal  
Neuropsychopharmacology Reports     Open Access  
Reviews on Clinical Pharmacology and Drug Therapy     Full-text available via subscription  
SynOpen     Open Access  
Matrix Science Pharma     Open Access  
Contract Pharma     Full-text available via subscription  
Journal of Cellular Neuroscience and Oxidative Stress     Open Access  
Istanbul Journal of Pharmacy     Open Access  
Acta Pharmaceutica Indonesia     Open Access  
Indonesian Journal of Pharmacy     Open Access  
EMC - Cosmetologia Medica e Medicina degli Inestetismi Cutanei     Full-text available via subscription  
High-Throughput     Open Access  
Scientia Pharmaceutica     Open Access  
Trends in Peptide and Protein Sciences     Open Access  
Acta Physiologica Hungarica     Full-text available via subscription  
Jurnal Farmasi Sains dan Praktis     Open Access  
Jurnal Kefarmasian Indonesia     Open Access  
Pharmaceutical Historian     Open Access  
Planta Medica International Open     Open Access  
Archives of Razi Institute     Open Access  
Journal of Pharmaceutical Research     Open Access  
EJNMMI Radiopharmacy and Chemistry     Open Access  
FarmaJournal     Open Access  
Journal of Negative and No Positive Results     Open Access  
Pharmaciana     Open Access  
Folia Medica Indonesiana     Open Access  
Актуальні питання фармацевтичної та медичної науки та практики     Open Access  
International Journal of Pharmacology, Phytochemistry and Ethnomedicine     Open Access  
Фармацевтичний часопис     Open Access  
Ciência Equatorial     Open Access  
Iranian Journal of Pharmaceutical Research     Open Access  
Research & Reviews : A Journal of Drug Design & Discovery     Full-text available via subscription  
Asian Journal of Medical and Pharmaceutical Researches     Open Access  
Research & Reviews : A Journal of Pharmacognosy     Full-text available via subscription  
Ars Pharmaceutica     Open Access  
Pharmacognosy Communications     Partially Free  
Egyptian Pharmaceutical Journal     Open Access  
Pharmacological Reports     Hybrid Journal  
PZ Prisma : Materialien zur Fort- und Weiterbildung     Full-text available via subscription  
Chinese Journal of Pharmaceutical Analysis     Full-text available via subscription  
Journal of Nanopharmaceutics and Drug Delivery     Full-text available via subscription  
Journal of Hydrogels     Full-text available via subscription  
Manufacturing Chemist     Full-text available via subscription  
Pharmaceutica Analytica Acta     Open Access  
Journal of Pharmacovigilance     Open Access  
Current Pharmacology Reports     Hybrid Journal  
Nigerian Journal of Natural Products and Medicine     Full-text available via subscription  
International Journal of Herbs and Pharmacological Research     Open Access  
Bangladesh Journal of Physiology and Pharmacology     Open Access  
Research Journal of Pharmacognosy     Open Access  

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Similar Journals
Journal Cover
Trends in Peptide and Protein Sciences
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2538-2535 - ISSN (Online) 2538-2446
Published by Shahid Beheshti University of Medical Sciences Homepage  [20 journals]
  • Author Package, TPPS, Vol. 7 (2022)

    • Authors: Maryam Tabarzad
      Pages: 1 - 16
      Abstract: The Trends in Peptide and Protein Sciences is a peer-reviewed, online-only (previously print-online), scientific journal owned by Protein Technology Research Center, Shahid Beheshti University of Medical Sciences and documents in all important aspects of the research in peptides and proteins focusing on analytics and impurities, bioinformatics, biopharmaceuticals and vaccines, biotechnology, chemical synthesis, conformational analysis, design and  development of protein therapeutics, determination of structure, enzymology, folding and sequencing,  formulation and stability, function, genetics,  immunology, kinetics, modeling, molecular biology, pharmacokinetics and pharmacodynamics of therapeutic proteins and antibodies, pharmacology,  protein engineering and development, protein-protein interaction, proteomics, purification/expression/production, simulation, thermodynamics and  hydrodynamics and protein biomarkers. The aim of this Journal is to publish high quality original research articles, reviews, short communications and letters and to provide a medium for scientists and researchers to share their findings from the area of peptides and proteins. The Trends in Peptide and Protein Sciences is published in collaboration with Iranian Association of Pharmaceutical Scientists. From volume 3 (2018) of TPPS, articles are continuously published online only, as soon as the review process is completed.
      PubDate: 2023-01-02
      DOI: 10.22037/tpps.v7i.40500
      Issue No: Vol. 7 (2023)
  • Electrosynthesis, in Silico Ligand-Protein Interaction and Pharmacokinetic
           Studies of Some Aryl Sulfone Derivatives as Potential Acetylcholinesterase

    • Authors: Omid Ghanbarzadeh, Farzad Kobarfard, Salimeh Amidi
      Pages: 1 - 7 (e11)
      Abstract: Acetylcholinesterase (AChE) is an enzyme that catalyzes the hydrolysis of acetylcholine. Acetylcholine plays an essential role in learning and memory. Since acetylcholine deficiency is related to the pathogenesis of Alzheimer's disease, obtaining acetylcholinesterase inhibitors is of great interest. Here, a group of aryl sulfone derivatives with a catechol ring was synthesized by the electrochemical method. The structure of compounds was confirmed by 1HNMR and ESI-MS. Molecular docking studies of the synthesized compounds with AChE were performed and the results showed that all of the compounds have good theoretical affinities for the target enzyme, AChE. Moreover, the physicochemical properties and ADME parameters of synthesized compounds were predicted, which revealed that they have acceptable features as drug candidates. HIGHLIGHTS
      Aryl sulfone derivatives with catechol rings were synthesized by electrochemical method.
      Molecular docking studies of the compounds revealed good binding affinities against acetylcholinesterase.
      All of the synthesized compounds obey Lipinski’s rule of five which suggests them as good oral drug candidates.
      PubDate: 2022-12-26
      DOI: 10.22037/tpps.v7i.40074
      Issue No: Vol. 7 (2022)
  • In-house Optimization Radiolabeling of Recombinant scFv with
           99mTc-Tricarbonyl and Stability Studies

    • Authors: Safura Joukar, Negar Bozorgchami, Dara Hatamabadi, Abdolreza Yazdani, Elham Mohit, Elmira Javani, Mohammadsaeed Kordi, Sepideh Khoshbakht, Soraya Shahhosseini
      Pages: 1 - 6 (e10)
      Abstract: His-tagged scFv fragments of monoclonal antibodies have better pharmacokinetic properties than whole antibodies. Radiolabeled scFvs are considered for targeted imaging and treatment. Technetium tricarbonyl provides radiolabeling of scFvs without losing its biological activity in a fast and easy procedure. Technetium tricabonyl was prepared as follows: A freshly eluted solution of Na99mTcO4 was added to a mixture containing sodium carbonate, sodium potassium tartarate, boranocarbonate, sodium borohydride. The mixture was heated for 30 min at 100°C. Radiochemical purity was determined using radio thin lyer chromatography. Then, technetium tricarbonyl was added to a solution of scFv in PBS buffer and incubated for 2 h at 50°C, purified by PD-10 column and radiochemical purity was determined. Results showed that radiochemical purity of technetium tricarbony was over 98%. The best conditions for radiolabeling of scFv was: scFv concentration >2 mg/mL, PBS buffer, 2 h incubation at 50°C, pH 8-9, and high activity concentration of tricarbonyl. The best radiochemical purity of scFv was 70% before purificarion. Radiolabeled scFv was stable in PBS for 24 h incubation and there was no release of technetium in competition with histidine. In this study, we optimized radiolabeling of a scFv with technetium tricarbonyl using house made boranocarbonates. The results are promising and will be used for future studies. HIGHLIGHTS
      Radiolabeling of scFv was done directly by 99mTc-tricarbonyl.
      99mTc-tricarbonyl was prepared in house from boranocarbonate.
      99mTc-Radiolabeled scFv can be used for radioimmunoscintigraphy.
      PubDate: 2022-11-12
      DOI: 10.22037/tpps.v7i.39823
      Issue No: Vol. 7 (2022)
  • Antimicrobial Peptide Design, Molecular Docking and ADMET Studies Against
           the Methicillin-Resistant Staphylococcus aureus and Carbapenem-resistant
           and Carbapenemase-producing Pseudomonas aeruginosa

    • Authors: Abdulmalik Aliyu, Yakubu K.E Ibrahim, Babajide A Tytler, Ahmed Olowo-okere
      Pages: 1 - 8 (e9)
      Abstract: Carbapenem-resistant and carbapenemase-producing Pseudomonas aeruginosa (CRPA) and methicillin-resistant Staphylococcus aureus (MRSA) are two pathogens that are resistant to currently available antimicrobials. As an alternative to effective medication molecules, antimicrobial peptides (AMPs) have the potential to cure superbug-caused infections effectively. Two new AMPs (ama1 and ama2) were designed utilizing a knowledge-based technique with optimal parameters. First, the PEP-FOLD 3.5 server made a de novo prediction of the AMPs' three-dimensional (3D) structure, which was validated using PROCHECK of SAVES v6.0 by checking the amino acid locations in the Ramachandra plot. Then, protein-peptide docking simulations of the predicted AMPs and reference AMP (Aurein 1.2) for positive control were performed using the HPEPDOCK docking web server, followed by the computation of the AMPs' physicochemical parameters and toxicity profile using the ProtParam and vNN-ADMET web servers, respectively. The sequences for ama1 and ama2 were AWGKIKALR and IKWLRLAKP, respectively. Docking analysis revealed that the antibacterial activity of ama1 and ama2 was superior to that of Aurein 1.2 against CRPA-resistant enzyme (6ew3), respectively. However, ama1, ama2, and Aurein 1.2 inhibited the activity of MRSA-resistant protein (4c12). Both the physicochemical qualities and the toxicity profiles were advantageous. Therefore, the in-silico-derived AMPs could serve as a pharmaceutical candidate for developing multidrug-resistant bacteria-effective antimicrobials. HIGHLIGHTS
      Two cationic antimicrobial peptides (AMPs) were designed.
      Molecular docking of the AMPs revealed better antimicrobial activity than the reference.
      The novel AMPs had net positive charge and optimal hydrophobic amino acids.
      PubDate: 2022-11-02
      DOI: 10.22037/tpps.v7i.39110
      Issue No: Vol. 7 (2022)
  • Synthesis, Radiolabeling and Stability Studies of Peptide
           HYNIC-LIKKP-Pyr-F with 99mTc as an Apoptosis Imaging Agent

    • Authors: Elmira Javani Jouni, Mohammadsaeed Kordi, Sepideh Khoshbakht, Salimeh Amidi , Soraya Shahhosseini
      Pages: 1 - 5 (e8)
      Abstract: A non-invasive method for detecting phosphatidylserine (PS) exposure on the outer surface of plasma membranes, such as nuclear imaging, could aid in the diagnosis and treatment of diseases associated with apoptosis. Annexin V has been the most researched imaging agent for apoptosis to date. Due to Annexin V's limitations, additional agents, such as small peptides and molecules, have been introduced, including LIKKPF developed by Burtea et al. In this study, HYNIC-LIKKP-pyr-F, a derivative of LIKKPF was prepared using the 9-fluoroenylmethoxycarbonyl (fmoc) method, radiolabeled with Technetium-99m (99mTc) with the use of Stannous chloride (SnCl2) as a reducing agent and ethylenediamine diacetate (EDDA) and tricine as co-ligands. Radiochemical purity, labeling efficiency, and stability of radiopeptide in normal saline and human plasma were determined using thin layer chromatography (TLC). The partition coefficient of radiolabeled peptide was measured in a combination of PBS (pH 7.4) and n-octanol. Specific activity was also measured. LC-MS was used to examine the synthesized peptide. Peptide was stable in human serum for at least 4 hr. Peptide was radiolabeled with 99mTc with radiochemical purity and labeling efficiency over 95% and 90%, respectively. Radiopeptide was stable in saline and human serum for at least 4 hours. The radiolabeled peptide has a great deal of potential as an apoptosis imaging agent for in vitro and in vivo experiments. HIGHLIGHTS
      A noninvasive method for apoptosis imaging is the usage of radiolabeled affinity ligands.
      A new derivative of LIKKPF with affinity for phosphatidyl serine was synthesized.
      The LIKKPF peptide was radiolabeled with 99m
      PubDate: 2022-09-13
      DOI: 10.22037/tpps.v7i.39024
      Issue No: Vol. 7 (2022)
  • Cytoplasmic Expression of Human Bone Morphogenetic Protein-7 by a
           Genetically Engineered Strain of Escherichia coli, SHuffle® Strain

    • Authors: Alireza Dugmehchi, Ghazal Sadipour, Yeganeh Talebkhan, Hoda Jahandar, Fahimeh Nemati, Elham Mohit, Leila Nematollahi
      Pages: 1 - 7 (e7)
      Abstract: Homodimeric bone morphogenetic protein-7 (BMP-7) plays a key role in bone metabolism. The functionality of human BMP-7 protein is dependent on its disulfide bond formation and proper folding. Therefore, the expression of soluble recombinant BMP-7 using Escherichia coli cells as the host remains a challenge. Given the need for these disulfide-bonded proteins for stabilized native conformation, the cytoplasm of SHuffle® T7 Express as an E. coli engineered strain can effectively fold disulfide-bonded proteins with a need for proper oxidative folding. These cellular features turn the SHuffle® expression system into an efficient host for the recombinant production of human BMP-7 protein. A soluble dimeric form of recombinant human BMP-7 (rhBMP-7) which has a wide range of applications in medicine and can be used in the treatment of bone defects was produced using the SHuffle® strain as the expression system. This study demonstrated the production of rhBMP-7 using E. coli SHuffle® T7 Express strain. Also, an effective protocol was proposed for the expression and purification of soluble human BMP-7. In addition, it was found that the genetically engineered SHuffle® strain can efficiently enhance the solubility of recombinant human BMP-7 as a therapeutic target. HIGHLIGHTS                                                             
      E. coli SHuffle® T7 Express strain is an effective host to express disulfide-bonded proteins.
      BMP-7 is involved in the process of bone formation.
      Expression of human BMP-7 in SHuffle® strain increased its solubility.  
      PubDate: 2022-09-06
      DOI: 10.22037/tpps.v7i.39039
      Issue No: Vol. 7 (2022)
  • Antioxidant Activity of Peptides Derived from Enzymatic Digestion of
           Spirulina platensis Protein Extract by Different Proteases

    • Authors: Hanieh Niknam, Fariba Fathi, Arash Mahboubi, Maryam Tabarzad
      Pages: 1 - 7 (e6)
      Abstract: One of the attractive sources of bioactive compounds is cyanobacteria and in particular, Chlorella vulgaris and Spirulina platensis. Enzymatic digestion of the Spirulina protein extract can result in bioactive peptides with diverse activities, including antioxidant function. This study aims to produce peptides with antioxidant properties after the enzymatic digestion of Spirulina platensis protein extract using three enzymes: bacterial protease, pepsin, and papain. The protein extract from Spirulina platensis was subjected to enzyme hydrolysis for 3 hours at 37°C (pH 7.4 for papain and bacterial protease and pH 5 for pepsin). The concentration of peptide fragments was evaluated to determine the yield of protein digestion. In order to measure the level of anti-oxidative potential of the hydrolysates, the DPPH assay was run. The results indicated that the bacterial protease led to the highest concentration of peptide fragments, while the hydrolysate obtained from pepsin digestion showed the most antioxidant activity (80%), mainly the peptides that have molecular weights less than 14 KDa. Consequently, pepsin can be a proper enzyme to produce antioxidant peptides from the protein extract of S. platensis. In conclusion, the results of the study confirmed that the products of enzymatic digestion by different enzymes have distinct features. HIGHLIGHTS
      Spirulina platensis protein extract was digested with three types of protease.
      Digestion of protein extract by pepsin resulted in higher antioxidant activity.
      Digestion by bacterial protease from Bacillus licheniformis resulted in higher yield of peptide formation.
      PubDate: 2022-08-24
      DOI: 10.22037/tpps.v7i.38965
      Issue No: Vol. 7 (2022)
  • Leginsulin Peptide from Legumes as a Ligand of Growth Hormone-Releasing
           Hormone Receptor for Lipodystrophy Management in Patients with AIDS

    • Authors: Mohsen Danaeifar
      Pages: 1 - 7 (e5)
      Abstract: Since the appearance of acquired immunodeficiency syndrome (AIDS) disease, millions of people got infected, thousands are died and many suffered from its complications. One of the chronic and late symptoms of AIDS is lipodystrophy that leads to losing of fat in some parts of the body while gaining it at other organs and sites. One of the main targets in drug development for management of lipodystrophy is growth hormone-releasing hormone (GHRH) receptor. As a secondary metabolite from plants, leginsulin is a peptide with 37 amino acids and considered as hormone-like peptide. In this study, through in silico approaches, binding of leginsulin and GHRH receptor was studied. The results showed strong binding of the two molecules with docking score of -324.16 and ligand RMSD of 47.26. The molecular dynamic investigation also revealed these two proteins remained bound for almost 104 ns. Evaluation of the peptide toxicity in the body had shown that it is not toxic to the human organs and also, it doesn’t pass through the blood brain barrier. The results support the use of legumes as a source of leginsulin for potential management of lipodystrophy in the patients with AIDS. HIGHLIGHTS
      Leginsulin is a plant secondary metabolite with 37 amino acids peptide structure.
      Binding of leginsulin and GHRH receptor was studied through in silico approaches.
      Results support the leginsulin as a potential management of lipodystrophy in AIDS patients.
      PubDate: 2022-06-22
      DOI: 10.22037/tpps.v7i.38445
      Issue No: Vol. 7 (2022)
  • Controversy Between In Vitro Biological Activities of a Novel Designed
           Antimicrobial Peptide and Its In Silico Predicted Activities

    • Authors: Fariba Fathi , Maryam Ghobeh , Arash Mahboubi, Maryam Tabarzad
      Pages: 1 - 12 (e4)
      Abstract: Due to their unique mechanisms of action, antimicrobial peptides (AMPs) are promising candidates to combat different infectious diseases. They usually non-specifically interact with the bacterial cell membrane, create pores in their membrane and increase its permeability which causes the death of pathogens. In the design and development of AMPs, in silico strategies have been developed to enhance the function and activity of natural peptides. In this study, in silico approaches were used to develop a novel AMP with several extra bioactivities. Then, the designed AMP were analyzed through computational methods by in vitro experiments. Bioinformatics research revealed a 10-amino-acid peptide (LVSARIRCPK) having antibacterial, anti-biofilm, antiviral, antifungal, and anti-inflammatory effects. However, only the antiviral capabilities of the peptide were validated in the experimental analysis of antibacterial, antifungal, and antiviral activities. This data suggests that; while bioinformatics approaches have greatly advanced in recent years, more optimization work has to be done in order to attain high accuracy and minimize mistakes. HIGHLIGHTS
      A novel 10 residues anti-microbial peptide was designed using bioinformatics tools.
      In vitro analysis showed that this novel AMP did not have efficient antimicrobial activities.
      Stringencies of bioinformatics criteria thresholds setting may result in better design.
      PubDate: 2022-06-08
      DOI: 10.22037/tpps.v7i.38346
      Issue No: Vol. 7 (2022)
  • Expression of the Mouse HSP27 Chaperone in CHO-K1 Cells for the
           Enhancement of Viable Cell Density in Batch Culture

    • Authors: Mohammad Reza Amini, Azam Rahimpour, Reyhaneh Hoseinpoor, Masoumeh Rajabibazl
      Pages: 1 - 5 (e2)
      Abstract: Chinese hamster ovary (CHO) cells are extremely vulnerable to cell viability loss in culture despite the availability of different nutrients supplementation strategies. As a result, extending the culture lifetime can profoundly increase recombinant protein expression. Overexpression of HSP27 and its anti-apoptotic effects have been shown in human cell lines in previous studies. In the current study, mouse HSP27 (mHSP27) was cloned in pcDNA 3.1 hygro expression vector and was expressed in CHO-K1 cells to assess its impacts on cell viability and growth. Expression of mHSP27 in CHO-K1 cells was confirmed using RT-PCR. A 3-fold enhancement in peak viable cell density of mHSP27 transfected clones was observed, and culture viability loss was delayed by 2 days compared to un-transfected cells. In future studies, the resulting mHSP27 CHO-K1 cells could be employed as a novel host system for the transient and stable expression of therapeutic recombinant proteins. HIGHLIGHTS
      Cell engineering is an effective strategy to cope with apoptosis in CHO cells.
      HSP27 is involved in mammalian cell apoptosis.
      Expression of the mouse HSP27 increased the viability and cell density of CHO-K1 cells.
      PubDate: 2022-03-09
      DOI: 10.22037/tpps.v7i.37418
      Issue No: Vol. 7 (2022)
  • Preparation and In Vitro Characterization of Crocin-loaded Casein

    • Authors: Fatemeh Mehryab, Fatemeh Taghizadeh, Azadeh Haeri
      Pages: 1 - 9 (e3)
      Abstract: Crocin, the main active constituent of saffron, has many important biological activities. Due to its anti-inflammatory properties, crocin can be potentially effective in different pathological conditions including oral ulcers. Novel drug delivery systems such as hydrogels have been used to increase the stability of crocin and provide a controlled release of this compound. Casein is the main protein of milk that possesses suitable properties for the fabrication of hydrogels. In this paper, casein-based hydrogels with different casein to crocin weight ratios were synthesized using the acid-gelation method. The prepared crocin-loaded hydrogels were characterized regarding their rheological behavior, drug content, swelling ratio, surface morphology, thermal stability, and in vitro release profile. The structure of casein hydrogels was characterized using Fourier transform infrared and X-ray diffraction. All formulations exhibited a pseudoplastic rheological behavior and there was no statistically significant difference in viscosity among them. Hydrogel with casein to crocin weight ratio of 10:1 had larger pores and demonstrated a higher swelling percentage and suitable thermal stability. All casein-based hydrogels demonstrated a slow release of crocin over 24 hours and the hydrogel with lower casein to crocin weight ratio had an increased release rate. Taken together, casein-based hydrogels were found to be effective carriers to provide a controlled release system for crocin delivery. HIGHLIGHTS
      Casein-based hydrogels were developed for delivery of crocin.
      Casein-based hydrogels provided a controlled in vitro release profile for crocin.
      Hydrogel with a lower casein ratio exhibited a higher release rate of crocin.
      PubDate: 2022-03-09
      DOI: 10.22037/tpps.v7i.37420
      Issue No: Vol. 7 (2022)
  • Risk of Outbreak Omicron Virus by Increasing the Number of Mutations in
           the Receptor Binding Domain (RBD)

    • Authors: Mehdi Pooladi, Soheila Karani
      Pages: 1 - 3 (e1)
      Abstract: HIGHLIGHTS
      Rapid spread of Omicron variant is a serious challenge.
      More than 50 mutations have occurred in Omicron variant.
      The mutations have caused the RBD structure to undergo significant changes
      PubDate: 2022-03-07
      DOI: 10.22037/tpps.v7i.37447
      Issue No: Vol. 7 (2022)
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