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  Subjects -> PHARMACY AND PHARMACOLOGY (Total: 575 journals)
Showing 1 - 200 of 253 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 25)
AAPS Open     Open Access   (Followers: 5)
AAPS PharmSciTech     Hybrid Journal   (Followers: 6)
AboutOpen     Open Access  
ACS Pharmacology & Translational Science     Hybrid Journal   (Followers: 3)
Acta Pharmaceutica     Open Access   (Followers: 4)
Acta Pharmaceutica Indonesia     Open Access  
Acta Pharmaceutica Sinica B     Open Access   (Followers: 1)
Acta Pharmacologica Sinica     Hybrid Journal   (Followers: 3)
Acta Physiologica Hungarica     Full-text available via subscription  
Actualites Pharmaceutiques     Full-text available via subscription   (Followers: 4)
Advanced Drug Delivery Reviews     Hybrid Journal   (Followers: 94)
Advanced Herbal Medicine     Open Access   (Followers: 8)
Advanced Therapeutics     Hybrid Journal   (Followers: 1)
Advances in Pharmacoepidemiology & Drug Safety     Open Access   (Followers: 2)
Advances in Pharmacological and Pharmaceutical Sciences     Open Access   (Followers: 9)
Advances in Pharmacology     Full-text available via subscription   (Followers: 17)
Advances in Pharmacology and Pharmacy     Open Access   (Followers: 5)
Advances in Traditional Medicine     Hybrid Journal   (Followers: 3)
Adverse Drug Reaction Bulletin     Full-text available via subscription   (Followers: 5)
AJP : The Australian Journal of Pharmacy     Full-text available via subscription   (Followers: 8)
Alternatives to Laboratory Animals     Full-text available via subscription   (Followers: 9)
American Journal of Cardiovascular Drugs     Hybrid Journal   (Followers: 19)
American Journal of Drug Discovery and Development     Open Access   (Followers: 3)
American Journal of Health-System Pharmacy     Full-text available via subscription   (Followers: 55)
American Journal of Pharmacological Sciences     Open Access   (Followers: 1)
American Journal of Pharmacology and Toxicology     Open Access   (Followers: 23)
American Journal of Therapeutics     Hybrid Journal   (Followers: 13)
Analytical Methods     Hybrid Journal   (Followers: 8)
Annales Pharmaceutiques Francaises     Full-text available via subscription  
Annals of Pharmacotherapy     Hybrid Journal   (Followers: 56)
Annual Review of Pharmacology and Toxicology     Full-text available via subscription   (Followers: 37)
Anti-Infective Agents     Hybrid Journal   (Followers: 5)
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry     Hybrid Journal   (Followers: 5)
Antibiotics     Open Access   (Followers: 11)
Antibody Therapeutics     Open Access  
Antiviral Chemistry and Chemotherapy     Open Access   (Followers: 1)
Antiviral Research     Hybrid Journal   (Followers: 8)
Applied Clinical Trials     Full-text available via subscription   (Followers: 7)
Archiv der Pharmazie     Hybrid Journal   (Followers: 2)
Archives of Drug Information     Hybrid Journal   (Followers: 4)
Archives of Pharmacal Research     Full-text available via subscription   (Followers: 2)
Archives of Pharmacy and Pharmaceutical Sciences     Open Access   (Followers: 1)
Archives of Razi Institute     Open Access  
Archivos Venezolanos de Farmacología y Terapéutica     Open Access  
Ars Pharmaceutica     Open Access  
Asian Journal of Medical and Pharmaceutical Researches     Open Access  
Asian Journal of Pharmaceutical Research and Health Care     Open Access   (Followers: 2)
Asian Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Asian Journal of Pharmaceutics     Open Access   (Followers: 1)
Asian Journal of Research in Medical and Pharmaceutical Sciences     Open Access  
ASSAY and Drug Development Technologies     Hybrid Journal   (Followers: 3)
Australian Journal of Herbal Medicine     Full-text available via subscription   (Followers: 4)
Australian Pharmacist     Full-text available via subscription   (Followers: 7)
Autonomic & Autacoid Pharmacology     Hybrid Journal  
Avicenna Journal of Phytomedicine     Open Access   (Followers: 1)
Bangladesh Journal of Pharmacology     Open Access  
Bangladesh Journal of Physiology and Pharmacology     Open Access  
Bangladesh Pharmaceutical Journal     Full-text available via subscription  
Basic & Clinical Pharmacology & Toxicology     Hybrid Journal   (Followers: 14)
Behavioural Pharmacology     Hybrid Journal   (Followers: 2)
Bioanalysis     Full-text available via subscription   (Followers: 11)
Biochemical Pharmacology     Hybrid Journal   (Followers: 10)
BioDrugs     Full-text available via subscription   (Followers: 8)
Biological & Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 3)
Biomarkers in Drug Development     Partially Free   (Followers: 2)
Biomaterials     Hybrid Journal   (Followers: 55)
Biomedical and Environmental Sciences     Full-text available via subscription   (Followers: 1)
Biomedicine & Pharmacotherapy     Full-text available via subscription   (Followers: 2)
Biometrical Journal     Hybrid Journal   (Followers: 9)
Biopharm International     Full-text available via subscription   (Followers: 20)
Biopharmaceutics and Drug Disposition     Hybrid Journal   (Followers: 10)
BMC Pharmacology     Open Access   (Followers: 2)
BMC Pharmacology & Toxicology     Open Access   (Followers: 8)
Brazilian Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
British Journal of Clinical Pharmacology     Hybrid Journal   (Followers: 30)
British Journal of Pharmacology     Hybrid Journal   (Followers: 17)
British Journal of Pharmacy (BJPharm)     Open Access   (Followers: 2)
Bulletin of Faculty of Pharmacy, Cairo University     Open Access   (Followers: 2)
CADTH Technology Overviews     Free  
Canadian Journal of Pain     Open Access   (Followers: 3)
Canadian Journal of Physiology and Pharmacology     Hybrid Journal   (Followers: 2)
Canadian Pharmacists Journal / Revue des Pharmaciens du Canada     Hybrid Journal   (Followers: 3)
Cancer Biotherapy & Radiopharmaceuticals     Hybrid Journal  
Cancer Chemotherapy and Pharmacology     Hybrid Journal   (Followers: 4)
Cardiovascular Drugs and Therapy     Hybrid Journal   (Followers: 14)
Cardiovascular Therapeutics     Open Access   (Followers: 3)
Cephalalgia Reports     Open Access  
Chemical and Pharmaceutical Bulletin     Full-text available via subscription   (Followers: 1)
Chemical Research in Toxicology     Hybrid Journal   (Followers: 22)
ChemMedChem     Hybrid Journal   (Followers: 9)
Chemotherapy     Full-text available via subscription   (Followers: 3)
Chinese Herbal Medicines     Full-text available via subscription   (Followers: 1)
Chinese Journal of Pharmaceutical Analysis     Full-text available via subscription  
Ciencia e Investigación     Open Access  
Ciência Equatorial     Open Access  
Clinical and Experimental Pharmacology and Physiology     Hybrid Journal   (Followers: 7)
Clinical and Translational Science     Open Access   (Followers: 4)
Clinical Complementary Medicine and Pharmacology     Open Access  
Clinical Drug Investigation     Full-text available via subscription   (Followers: 8)
Clinical Medicine Insights : Therapeutics     Open Access  
Clinical Neuropharmacology     Hybrid Journal   (Followers: 2)
Clinical Pharmacist     Partially Free   (Followers: 12)
Clinical Pharmacokinetics     Full-text available via subscription   (Followers: 28)
Clinical Pharmacology & Therapeutics     Hybrid Journal   (Followers: 45)
Clinical Pharmacology in Drug Development     Hybrid Journal   (Followers: 4)
Clinical Pharmacology: Advances and Applications     Open Access   (Followers: 6)
Clinical Research and Regulatory Affairs     Hybrid Journal   (Followers: 12)
Clinical Therapeutics     Hybrid Journal   (Followers: 34)
Clinical Toxicology     Hybrid Journal   (Followers: 18)
Clinical Trials     Hybrid Journal   (Followers: 18)
CNS Drug Reviews     Open Access   (Followers: 4)
CNS Drugs     Full-text available via subscription   (Followers: 10)
Combination Products in Therapy     Open Access  
Consultant Pharmacist     Full-text available via subscription   (Followers: 2)
Consumer Drugs     Full-text available via subscription  
Contract Pharma     Full-text available via subscription  
Cosmetics     Open Access   (Followers: 4)
CPT : Pharmacometrics & Systems Pharmacology     Open Access   (Followers: 11)
Critical Reviews in Clinical Laboratory Sciences     Hybrid Journal   (Followers: 16)
Critical Reviews in Therapeutic Drug Carrier Systems     Full-text available via subscription   (Followers: 5)
Critical Reviews in Toxicology     Hybrid Journal   (Followers: 25)
Current Bioactive Compounds     Hybrid Journal  
Current Cancer Therapy Reviews     Hybrid Journal   (Followers: 5)
Current Clinical Pharmacology     Hybrid Journal   (Followers: 4)
Current Drug Delivery     Hybrid Journal   (Followers: 6)
Current Drug Discovery Technologies     Hybrid Journal   (Followers: 6)
Current Drug Metabolism     Hybrid Journal   (Followers: 5)
Current Drug Safety     Hybrid Journal   (Followers: 8)
Current Drug Targets     Hybrid Journal   (Followers: 5)
Current Drug Therapy     Hybrid Journal   (Followers: 3)
Current Enzyme Inhibition     Hybrid Journal   (Followers: 1)
Current Issues in Pharmacy and Medical Sciences     Open Access   (Followers: 2)
Current Medical Science     Hybrid Journal  
Current Medicinal Chemistry     Hybrid Journal   (Followers: 13)
Current Molecular Pharmacology     Hybrid Journal  
Current Nanoscience     Hybrid Journal  
Current Neuropharmacology     Hybrid Journal   (Followers: 1)
Current Opinion in Pharmacology     Hybrid Journal   (Followers: 9)
Current Pharmaceutical Analysis     Hybrid Journal   (Followers: 1)
Current Pharmaceutical Biotechnology     Hybrid Journal   (Followers: 10)
Current Pharmaceutical Design     Hybrid Journal   (Followers: 11)
Current Pharmacogenomics and Personalized Medicine     Hybrid Journal   (Followers: 3)
Current Pharmacology Reports     Hybrid Journal  
Current Protocols in Pharmacology     Hybrid Journal  
Current Radiopharmaceuticals     Hybrid Journal   (Followers: 1)
Current Research in Drug Discovery     Open Access   (Followers: 2)
Current Research in Pharmacology and Drug Discovery     Open Access   (Followers: 1)
Current Therapeutic Research     Open Access   (Followers: 6)
Current trends in Biotechnology and Pharmacy     Open Access   (Followers: 8)
Current Vascular Pharmacology     Hybrid Journal   (Followers: 4)
Dhaka University Journal of Pharmaceutical Sciences     Open Access  
Die Pharmazie - An International Journal of Pharmaceutical Sciences     Full-text available via subscription   (Followers: 5)
Dose-Response     Open Access  
Drug and Chemical Toxicology     Hybrid Journal   (Followers: 13)
Drug and Therapeutics Bulletin     Hybrid Journal   (Followers: 8)
Drug Delivery     Open Access   (Followers: 8)
Drug Delivery and Translational Research     Hybrid Journal   (Followers: 2)
Drug Design, Development and Therapy     Open Access   (Followers: 3)
Drug Development and Industrial Pharmacy     Hybrid Journal   (Followers: 29)
Drug Development Research     Hybrid Journal   (Followers: 11)
Drug Discovery Today: Technologies     Full-text available via subscription   (Followers: 12)
Drug Metabolism and Disposition     Hybrid Journal   (Followers: 13)
Drug Metabolism and Pharmacokinetics     Hybrid Journal   (Followers: 6)
Drug Metabolism Letters     Hybrid Journal   (Followers: 3)
Drug Metabolism Reviews     Hybrid Journal   (Followers: 8)
Drug Research     Hybrid Journal   (Followers: 3)
Drug Resistance Updates     Hybrid Journal   (Followers: 3)
Drug Safety     Full-text available via subscription   (Followers: 80)
Drug Safety - Case Reports     Open Access   (Followers: 2)
Drug Target Insights     Open Access  
Drug, Healthcare and Patient Safety     Open Access   (Followers: 10)
Drugs     Full-text available via subscription   (Followers: 136)
Drugs & Aging     Full-text available via subscription   (Followers: 10)
Drugs & Therapy Perspectives     Full-text available via subscription   (Followers: 9)
Drugs : Real World Outcomes     Hybrid Journal   (Followers: 1)
Drugs and Therapy Studies     Open Access  
Drugs in R & D     Full-text available via subscription   (Followers: 2)
Drugs of the Future     Full-text available via subscription   (Followers: 8)
East and Central African Journal of Pharmaceutical Sciences     Open Access   (Followers: 1)
Egyptian Pharmaceutical Journal     Open Access  
EJNMMI Radiopharmacy and Chemistry     Open Access  
EMC - Cosmetologia Medica e Medicina degli Inestetismi Cutanei     Full-text available via subscription  
Emerging Trends in Drugs, Addictions, and Health     Open Access  
Environmental Toxicology and Pharmacology     Hybrid Journal   (Followers: 9)
Epilepsy Research     Hybrid Journal   (Followers: 7)
Ethiopian Pharmaceutical Journal     Full-text available via subscription   (Followers: 1)
EUREKA : Health Sciences     Open Access  
European Journal of Clinical Pharmacology     Hybrid Journal   (Followers: 14)
European Journal of Drug Metabolism and Pharmacokinetics     Hybrid Journal   (Followers: 8)
European Journal of Hospital Pharmacy : Science and Practice (EJHP)     Hybrid Journal   (Followers: 5)
European Journal of Medicinal Plants     Open Access   (Followers: 2)
European Journal of Pharmaceutical Sciences     Hybrid Journal   (Followers: 85)
European Journal of Pharmaceutics and Biopharmaceutics     Hybrid Journal   (Followers: 34)
European Journal of Pharmacology     Hybrid Journal   (Followers: 8)
European Neuropsychopharmacology     Hybrid Journal   (Followers: 9)
European Review for Medical and Pharmacological Sciences     Full-text available via subscription   (Followers: 1)
Experimental and Clinical Psychopharmacology     Full-text available via subscription   (Followers: 7)
Expert Opinion on Drug Delivery     Hybrid Journal   (Followers: 17)
Expert Opinion on Drug Discovery     Hybrid Journal   (Followers: 18)

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Journal Cover
Regulatory Mechanisms in Biosystems
Number of Followers: 1  

  This is an Open Access Journal Open Access journal
ISSN (Print) 2519-8521 - ISSN (Online) 2520-2588
Published by Oles Honchar Dnipropetrovsk National University Homepage  [7 journals]
  • The effect of vitamin E on the lipid environment of rat hepatocyte

    • Authors: S. B. Silonov, E. O. Kryvenko, N. B. Silonova, T. M. Shevchenko
      Pages: 91 - 98
      Abstract: Tocopherol is one of the known beneficial natural antioxidants ensuring the optimal level of functioning of mammalian organisms. Numerous in vitro and in vivo experiments have shown that the biological role of vitamin E is to prevent the development of pathologies caused by oxidative stress. In particular, the role of enzymatic factors of lipid peroxidation and related inflammation as a result of eicosanoid synthesis was clearly shown. We studied changes in the structural and functional state of hepatocyte membranes in the classical model of E-hypovitaminosis caused by long-term (70 days) insufficient intake of vitamin E in the diet of rats. The test components were determined spectrophotometrically after appropriate chromatographic procedures. The amount of total and individual leukotrienes was determined by ELISA. Prolonged tocopherol deficiency in rats caused a 49.4% decrease in tocopherol, more than 27.0% – in cholesterol. Of the 8 individual phospholipids studied, 6 showed significant changes: a decrease in cardiolipin and phosphatidylserine, and an increase in phosphatidylethanolamine by 3.24 times, an increse in lysophosphatidylcholine by 86.9%, in phospha­tidylcholine by 52.8%, and in sphingomyelin by 30.6%, relative to control. There were changes in the levels of unsaturated fatty acids playing a significant role in the development of functional disorders in cells and affecting the metabolism of ecosanoids derived from arachidonic acid by the 5-lipoxygenase oxidation pathway. Changes in the levels of total and individual cysteinyl leukotrienes in the state of E-hypovitaminosis were revealed. Restoration of vitamin E intake returns most of the studied indicators such as tocopherol, cholesterol, polyunsaturated fatty acids to the control levels and activates the processes of sequential conversion of leukotrienes in the body of rats. The obtained results indicate the potentiating effect of vitamin E on metabolic processes in the body as a whole and in hepatocytes and eicosanoid metabolism. The degree of tocopherol intake allows one to influence the course of inflammatory processes associated with eicosanoids, not only through the impact on precursors, but also on the utilization of metabolites, including leukotrienes.
      PubDate: 2022-05-28
      DOI: 10.15421/022213
      Issue No: Vol. 13, No. 2 (2022)
  • The experimental invasion of rats with Eustrongylides excisus (Nematoda,
           Dioctophymatidae) larvae during the acute course of infection

    • Authors: S. L. Honcharov, O. M. Kupriianova, N. M. Soroka, M. V. Halat, A. I. Dubovyi, O. V. Zhurenko
      Pages: 99 - 104
      Abstract: Ten laboratory rats were experimentally invaded per os with the Eustrongylides excisus (Jäegerskiold, 1909) nematode larvae (L3–L4) received from Perca fluviatialis (Linnaeus, 1758), caught in the water area of the Dnipro-Buh Estuary, Ukraine. The aim of the experimental study was to measure the pathogenic effect of the given parasite on the mammals’ organisms by means of the histological analysis of the affected organs of the infected rats. The researched animals were divided into two groups. The experiment lasted 15 days; the second group was a control group. The histological analysis showed purulent-serous and purulent-fibrinous peritonitis, acute circulatory disorders in brain tissues (signs of hypoxic-ischemic lesions of the brainstem in the form of neuronal changes) and the heart (partial spasm of arteries and edema of interstitial myocardial tissue); pathological changes in the lungs (acute interstitial purulent pneumonia, acute focal emphysema of the lungs), liver (acute exudative hepatitis), kidneys (signs of shock reaction) and intestines (acute serous-purulent enteritis). Formation of non-specific granulomas was noticed, which contained the fragments of the parasitic larvae. The acute course of the purulent-serous and purulent-fibrinous inflammatory processes in the organs of the invaded rats was noticed; signs of systemic inflammatory response syndrome with pronounced distributive vascular leukocytosis in the studied organs.
      PubDate: 2022-04-24
      DOI: 10.15421/022214
      Issue No: Vol. 13, No. 2 (2022)
  • Biological activity of soybean seed lectin at the spraying of Glycine max
           plants against the background of seed treatment with pesticide containing
           fipronil, thiophanate-methyl, pyraclostrobin as active substances and
           rhizobial bacterization

    • Authors: O. V. Kyrychenko, S. Y. Kots, A. V. Khrapova, S. V. Omelchuk
      Pages: 105 - 113
      Abstract: Preparations for protecting plants, particularly those with fungicidal activity, continue to be relevant in agricultural production. They are used to effectively combat phytopathogens and ensure high yield of cultivated plants. However, they are among the anthropogenic factors which impose a heavy chemical load on ecosystems. Data about the effects of pesticides on physiological parameters of plants are essential for understanding the main regulatory mechanisms as preconditions to the phytotoxic state of compounds, as well as greater substantial understanding of the functional condition and implementation of adaptive potential of plants during and post stress. An important and relevant task – both practically and theoretically in the conditions of action of fungicide on seeds - is studying the possibilities of application of phytolectines as biologically active compounds with broad spectrum of action, including fungicidal effect, for spraying legumes in order to stabilize their development, ensure effective functioning of legume-rhizobial symbioses and cause fuller realization of productive potential against the background of decrease in chemical pressure on agrocenoses. Therefore, we aimed to evaluate the biological activity of soybean seed lectin (according to the parameters of productivity and functional activity of soybean-rhizobial symbiosis) at the spraying of Glycine max (L.) Merr. plants against the background of seed treatment of pesticide, Standak Top with fungicidal and insecticidal actions on the day of sowing and inoculation with Bradyrhizobium japonicum 634b. We used physiological, biochemical, microbiological and statistical methods of studies. We determined that Standak Top, applied on soybeans that were afterwards inoculated with rhizobia, exerted negative tendency on formation of vegetative mass by plants at the beginning of vegetation, though in the following phases of ontogenesis, their development and productivity reached the level of the control. Nitrogenase activity of symbiosis and the condition of photosynthetic pigment complex (content of chlorophyll and carotenoids and their ratio) were at the level or significantly lower than in the control plants. After spraying soybeans with lectin (without use of the fungicide) in the phase of development of two true leaves, there occurred significant increase in functional activity of the symbiotic system (according to total nitrogenase activity of symbiosis, higher by 1.91 and 1.79 times compared with the controls with inoculation and inoculation + fungicide) and the content of photosynthetic pigments (chlorophylls were higher by 1.12–1.45 times, carotenoids by 1.14–1.39 times) and development of strong leaf apparatus (by 1.33–1.42 times). This caused highest level of realization (by 13.9% and 10.1% higher compared with the controls with inoculation and inoculation + fungicide) of productive potential of cultivated plants. After spraying plants with soybean lectin against the background of use of fungicide, notable and reliable increases occurred in the level of absorption of molecular nitrogen (by 1.72 and 1.52 times according to total activity of symbiosis, compared with the controls with inoculation and inoculation + fungicide), content of chlorophyll (1.25–1.64 times) and carotenoids (1.12–1.42 times) in leaves of soybean, and also plants were actively developing during vegetation (1.12–1.40 times), producing yield that exceeded by 12.8% and 9.1% the controls with inoculation and inoculation + fungicide. Therefore, use of soybean seed lectin for spraying plants against the background of seed treatment of pesticide Standak Top on day of sowing can stabilize and even increase the level of realization of symbiotic and productive potential of soybean-rhizobial symbiosis compared both with the control (inoculation with rhizobia) and the variant with treatment of seeds (rhizobia + fungicide). This indicates on the perspectives of further studies of biological activity of phytolectins aiming at decreasing chemical pressure on ecosystems by leveling out or decreasing the negative impact of chemical means of protection on the plants and symbiosis.
      PubDate: 2022-04-05
      DOI: 10.15421/022215
      Issue No: Vol. 13, No. 2 (2022)
  • Effects of interaction and effectiveness of weed control when using tank
           mixtures of herbicides in maize crops

    • Authors: V. V. Yukhymuk, M. P. Radchenko, S. K. Sytnik, Y. Y. Morderer
      Pages: 114 - 120
      Abstract: Many countries are now facing the problem of increase in resistant biotypes of weeds. The spread of herbicide-resistant weeds across agrophytocenoses poses a threat of decrease in the effectiveness of use of herbicides in agricultural fields. In order to develop anti-resistant compositions of herbicides for protection of maize (Zea mays L.) crops, we studied effects of interaction and efficiency of weed control in greenhouse and field experiments. We studied the possibility of combined use of 4-hydroxyphenyl pyruvate dioxygenase-inhibiting herbicide tolpyralate and inhibitor of transport of electrons in photosystem 2 of chloroplasts – terbuthylazine – and acetolactate synthase-inhibiting rimsulfuron. In greenhouse experiments on model objects, we found that interaction in the mixtures of tolpyralate with rimsulfuron was antagonistic, but the antagonism may be overcome by increasing the rate of applied rimsulfuron. At joint use of tolpyralate and terbuthylazine, a synergistic increase in phytotoxic effect was observed, caused by increase in the effectiveness of the blocking electron-transport chain and increase in intensity of formation of reactive oxygen species. According to the results of the field experiments, we drew the conclusion that the efficacy of using the mixture of tolpyralate and rimsulfuron depends on the species composition of weeds. In the presence of rimsulfuron-resistant weeds, interaction with tolpyralate becomes antagonistic even in the conditions of increased rate of application of rimsulfuron, and thus the effectiveness of the protection significantly decreases. At the same time, after applying tank mixture of tolpyralate with terbuthylazine, the synergistic character of the interaction was maintained toward a broad range of species of grass and dicotyledonous weeds, providing high efficiency of maize crop protection. The herbicide compositions that were analyzed and are presented in the article allow one to decrease the possibility of emergence of resistant biotypes of weeds, and also to effectively control the already existing resistant biotypes.
      PubDate: 2022-04-12
      DOI: 10.15421/022216
      Issue No: Vol. 13, No. 2 (2022)
  • Effects of copper citrate on physiological-biochemical parameters of
           ejaculate of sire boars

    • Authors: A. Shostya, A. Siabro
      Pages: 121 - 129
      Abstract: Revealing peculiarities of the course of physiological and biochemical processes in the formation of parameters of ejaculate allows effective correlation of biological completeness of spermatozoids based on the use of chelate compounds of limiting microelements, particularly copper. The study was focused on determining changes in processes of spermatogenesis during correction of mineral nutrition. In the experiment, we used adult boars of the same age, live weight and quality of ejaculates. They consumed copper citrate during the 45 day experiment in doses higher than the norm by 10% (1.5 mg per 1 kg of combined feed) and 20% (3 mg per 1 kg of combined feed) compared with the control. We determined difference in the effects of different doses of copper citrate on the formation of parameters of ejaculates in sire boars. Addition of the mineral additive to the feed in the doses 10% exceeding the norm promoted increase in the parameters of functional activity of spermatozoids – mobility and survival – on day 45 of the intake, which occurred against the background of acceleration of peroxidation processes (increase in the concentration of thiobarbituric acid of active compounds) and activation of the system of antioxidant protection – increase in the activity of superoxide dismutase and decrease in the level of catalase in the sperm. At the same time, we observed increase in the morphometric parameters of spermatozoids – general length, width, length and volume of the head. On day 30, sire boars that had been consuming copper citrate in the amounts 20% above the norm were observed to have increase in concentration of spermatozoids, their mobility and survivability, though a decrease was seen in the morphometric parameters of the gametes. Further intake of this element caused increase in the amount of abnormal forms of spermatozoids. Under the action of this additive, we determined increase in the amount of metabolites of lipid peroxidation – conjugated dienes and thiobarbituric acids of active compounds, and also weakening of the system of antioxidative protection – decrease in the content of reduced glutathione and accumulation of dehydroascorbic acid. Thus, the intake of copper citrate in the amounts of 10% above the norm facilitated morpho-functional parameters of spermatozoids by activating the system of antioxidant protection. It would be promising to conduct further research to determine the effects of copper citrate on the processes of activation and capacitation of spermatozoids and fertilization of oocytes in in vivo and in vitro conditions.
      PubDate: 2022-05-11
      DOI: 10.15421/022217
      Issue No: Vol. 13, No. 2 (2022)
  • Endophytic community of Chaenomeles speciosa fruits: Screening for
           biodiversity and antifungal activity

    • Authors: Y. V. Lykholat, O. O. Didur, O. A. Drehval, N. O. Khromykh, T. V. Sklyar, T. Y. Lykholat, O. V. Liashenko, I. M. Kovalenko
      Pages: 130 - 136
      Abstract: Fruit crops of the genus Chaenomeles Lindl are considered today as a superfood due to accumulation of biologically active compounds with antioxidant ability and known health-promoting properties. Successful introduction of this non-traditional culture in the steppe zone of Ukraine characterised by an unfavourable climate suggests the functioning of effective protective mechanisms in plants, including those that can be provided by the influence of endophytic microorganisms. However, there is little information about the endophytic community of Chaenomeles plants. Herein, the current study was aimed to isolate the endophytic fungi from the Ch. speciosa fruits and evaluate their biological activities against the phytopathogens. The study was carried out based on the collection of the Botanical Garden of Oles Honchar Dnipro National University (Dnipro city, Ukraine). Three media, namely PDA, MPA, and Gause’s medium were used for isolation of endophytic fungi. Colonies of isolates for identification were grown on PDA, Czapek's agar, and Czapek’s yeast autolysate media. Six fungal endophytic isolates derived from both peel and pulp of Ch. speciosa fruits have been morphologically identified using macroscopic and microscopic techniques, and assigned to the genus Penicillium (sections Chrysogena, Penicillium, Viridicata), and genus Talaromyces (section Talaromyces). Species P. expansum, P. viridicatum, and P. hirsutum were identified among the peel isolates, while P. chrysogenum, P. cyclopium, and P. purpurogenum were among the pulp isolates. Antagonistic ability of the endophytic isolates against phytopathogenic fungi was evaluated using the dual culture method. The results showed moderate to high antifungal capacity of the endophytic isolates against the phytopathogenic strains of the Fusarium genus. The growth inhibition of F. culmorum mycelium due to the influence of endophytic isolates was 51.5–81.3%, and the inhibition of the growth of F. oxysporum colonies was in the range of 68.4–86.6% as compared with control. There were no significant differences in the antagonistic ability between endophytic isolates derived from the peel and pulp of the fruit. Taken together, our findings indicated the great potential of the endophytic fungi from Ch. speciosa fruits as a source for the development of biocontrol agents and discovery of new bioactive compounds.
      PubDate: 2022-05-04
      DOI: 10.15421/022218
      Issue No: Vol. 13, No. 2 (2022)
  • Diagnostic traits of medicinal herbal raw material of species of Thymus

    • Authors: M. R. Hrytsyna, M. I. Skybitska, О. Т. Novikevich
      Pages: 137 - 152
      Abstract: The paper presents the peculiarities of morphological-anatomical structure of the organs, which are diagnostic traits of herbal raw materials (HRM) of the commonest species of Thymus L. genus in the moderate climatic zone. We determined that the studied species develop a subshrub life form, which grows monopodially in Th. pulegioides and Th. marschallianus, and sympodially with mono-, di- and polycyclic types of monocarpic shoots in Th. serpyllum. Inflorescence is of thyrsoid type with opposite partial inflorescences, which are constituents of simple dichasia with monochasia. Macroscopic and microscopic diagnostic features of the HRM are structure and pubescence on the stem, leaves and flowers, the anatomical structure of which was studied on 20 µm thick transversal sections using a light microscope. In Th. pulegioides, stems are tetrahedral, pubescent on ribs with unicellular and two-cellular elbow-shaped hairs. Leaves are ovoid, covered by multiangular epidermis that bears diacytic stromas, sharpened capitate unicellular and two-cellular hairs and 10–12 cellular essential oil glandules. In Th. marschallianus, the stems are poorly tetrahedral, rounded, pubescent on the entire surface. Leaves are elliptic, the cells of the epidermis are elongated, significantly tortuous, with 10–12 cellular glandules, diacytic stromas, capitate and unicellular hairs. In Th. serpyllum, the stems are cylindric, pubescent all round. The leaves are elongated-elliptic, epidermis cells are oval, tortuous, stromas are diacytic, essential oil glandules are 8–10 cellular, number of papilla-like hairs is low. The petioles and lamina base of the studied species are pubescent on the sides with short, coned and elbow-shaped, 2–3 cellular hairs, among which there are long, 4–8 cellular hairs; the hairs in Th. serpyllum are multicellular, interrupted. In mesophyll, there are schizogenous essential oil reservoirs. In Th. marschallianus and Th. pulegioídes, the calyx is campanulate, bilabiate, and pubescent. The flower corolla is purple-violet, bilabiate, has tube and outward bend, pubescent inside with coned unicellular hairs, has essential oil glandules and glandular capitate hairs, and pubescence in the fauces comprises long unicellular hairs. In Th. pulegioídes, cells of the corolla epidermis are multiangular-shaped with insignificantly wavy walls, in Th. marschallianus ‒ rectangular with significantly wavy walls. Cells of the tube epidermis are rectangular, tortuous. Surface of the corolla of Th. serpyllum is pubescent on both sides with short, cone-like and three-cellular, interrupted hairs, and also capitate hairs, and has essential oil glandules. Epidermis cells are multi-angular, tubes – rectangular and narrow. The obtained results may be used for diagnostics of HRM of morphologically similar species during their preparation.
      PubDate: 2022-03-11
      DOI: 10.15421/022219
      Issue No: Vol. 13, No. 2 (2022)
  • Influence of potassium dichromate on the reduction of sulfur, nitrate and
           nitrite ions by bacteria Desulfuromonas sp.

    • Authors: O. M. Moroz, S. O. Hnatush, H. V. Yavorska, G. I. Zvir, O. V. Tarabas
      Pages: 153 - 167
      Abstract: This article presents the regularities of reduction of sulfur, nitrate and nitrite ions by sulfur reducing bacteria Desulfuromonas sp., which were isolated from the water of the man-made Yavorivske Lake (Lviv Region, Ukraine), under the influence of potassium dichromate. This bacteria in the process of anaerobic respiration can use and reduce different electron acceptors, such as sulfur, nitrates, nitrites, oxidized forms of heavy metals, in particular, hexavalent chromium. Technogenically altered ecotopes are characterized by complex pollution, so several electron acceptors are available to bacteria at the same time. Strains of microorganisms isolated from such ecotopes are adapted to unfavourable conditions and therefore have high biotechnological potential. The purpose of this work was to investigate the regularities of elemental sulfur, nitrate or nitrite ion usage by sulfidogenic bacteria of Desulfuromonas genus in conditions of simultaneous presence in the medium of another electron acceptor – Cr(VI), to establish the succession of reduction of electron acceptors by strains of these bacteria and to evaluate the efficiency of their possible application in technologies of complex purification of the environment from metal compounds and other inorganic toxicants. Bacteria were grown under anaerobic conditions in Kravtsov-Sorokin medium without SO42– and without Mohr’s salt for 10 days. To study the efficiency of sulfur, nitrate or nitrite ions’ reduction at simultaneous presence in the medium of Cr(VI) bacteria were sown in media with elemental sulfur, NaNO3, NaNO2 or K2Cr2O7 to final S0, NO3–, NO2–or Cr(VI) concentration in the medium of 3.47 (concentration of SO42– in medium of standard composition) or 1.74, 3.47, 5.21, 6.94 and 10.41 mM. Biomass was determined by the turbidimetric method, and the concentrations of nitrate, nitrite, ammonium ions, hydrogen sulfide, Cr(VI), Cr(ІІІ) in cultural liquid were determined spectrophotometrically. It has been established that Cr(VI) inhibits the biomass accumulation and hydrogen sulfide production by bacteria of Desulfuromonas sp. after simultaneous addition into the medium of 3.47 mM S0 and 1.74–10.41 mM Cr(VI). In the medium with the same initial content (3.47 mM) of S0 and Cr(VI) bacteria produced Cr(III) at concentrations 3.3–3.4 times higher than that of hydrogen sulfide. It has been shown that K2Cr2O7 inhibits biomass accumulation, nitrate ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO3– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO3– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) of NO3– and Cr(VI) bacteria reduced up to 1.2 times more nitrate ions than Cr(VI) with the production of ammonium ions at concentrations the same times higher than those of Cr(III). It has been established that K2Cr2O7 inhibits biomass accumulation, nitrite ions’ reduction and ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO2– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO2– and 3.47 mM Cr(VI). In the medium with the same initial content of (3.47 mM) NO2– and Cr(VI) the reduction of Cr(VI) by bacteria was only slightly, up to 1.1 times, lower than the reduction of nitrite ions, almost the same concentrations of trivalent chromium and ammonium ions were detected in the cultural liquid. The processes of nitrate and nitride reduction carried out by bacteria of Desulfuromonas genus were revealed to be less sensitive to the negative influence of sodium dichromate, as compared with the process of sulfur reduction, because in the media with the same initial content (3.47 mM) of NO3– or NO2– and Cr(VI) bacteria produced 1.1–1.2 times more NH4+ than Cr(III), but in the medium with the same initial content (3.47 mM) of S0 and Cr(VI) ) bacteria produced over than three times more Cr(III) than hydrogen sulfide. Our data allow us to conclude that bacteria of Desulfuromonas genus, the investigated strains of which are adapted to high concentrations (up to 10.41 mM) of inorganic toxicants, play an important role in the geochemical cycles of sulfur, nitrogen and chromium in aquatic environments that have been under anthropogenic influence.
      PubDate: 2022-06-04
      DOI: 10.15421/022220
      Issue No: Vol. 13, No. 2 (2022)
  • Detection of Streptococcus suis using the optimized real-time polymerase
           chain reaction protocol

    • Authors: M. O. Savcheniuk, O. A. Tarasov, O. M. Zakharova, L. Y. Korniienko, V. M. Zotsenko, T. M. Tsarenko
      Pages: 168 - 173
      Abstract: The article presents the results of studies on the detection of Streptococcus suis by real-time polymerase chain reaction. Isolation and species identification of the studied isolates of streptococci was carried out according to morphological, cultural, biochemical and biological properties by conventional methods. The study of cultural characteristics of growth was carried out using conventional bacteriological methods on the brain heart infusion broth (BHI) and BHI agar with the addition of 5% sheep blood (blood BHI agar). To confirm biochemical properties as a confirmatory method, API 20 STREP test kit (bioMerieux, France) was used. In addition, to differentiate S. suis from the non-pathogenic species of streptococci, the hemolysis test was used. As a result of the studies, it was found that the use of the real-time PCR (polymerase chain reaction) method makes it possible to detect S. suis in an amount of 1 x 104 genome copies in the sample. All described validation parameters for the qualitative detection of S. suis DNA by real-time PCR meet international requirements, which guarantees accurate and reliable results. In Ukraine only a diagnostic test kit for convential PCR has been developed for the detection of swine streptococcosis. This approach is more time consuming and complex in comparison with the real-time PCR approach. We recommend that diagnostic laboratories implement this method in their practice. This will increase the number of effective diagnostic tools available to veterinarians on pig farms when they order laboratory tests. The high analytical sensitivity limit of a test is an essential parameter when screening is the focus, and obtaining false negative results causes a risk of the development of infection process among pig populations within infected herds. Our study showed that microbiological diagnostic methods to determine morphological and cultural properties can identify S. suis at the genus level. Determination of biochemical properties using the API 20 STREP test kit can be used to identify S. suis 1 and 2 serotypes. The conventional method and real-time PCR have 100% specificity and can be used to identify S. suis of different serotypes. Real-time PCR is a 2 to 4 times more sensitive limit than conventional PCR depending on the serotype being studied, and can be used to more accurately identify streptococcal DNA. It was found that the use of the real-time PCR method makes it possible to detect S. suis in an amount of 1 x 104 copies of the genome in the sample. Additionally, it was found that all the studied validation parameters of the qualitative method for determining S. suis DNA by real-time PCR meet international requirements, which guarantees accurate and reliable results.
      PubDate: 2022-05-18
      DOI: 10.15421/022221
      Issue No: Vol. 13, No. 2 (2022)
  • Potential use of synthetic and natural aromatic mixtures in prevention
           from Shelfordella lateralis сockroaches

    • Authors: O. V. Parhomenko, S. V. Kolomiichuk, D. D. Omelianov, V. V. Brygadyrenko
      Pages: 174 - 179
      Abstract: Attractive and repellent properties of many household chemicals may be used to combat synantrophic insects, such as cockroaches. In the natural environment, Shelfordella lateralis (Walker, 1868) (Blattodea, Blattidae) lives in the area spanning Central Asia to North Africa. Furthermore, in many tropical and subtropical countries, it is common in human accomodations. In the laboratory conditions, we determined reaction of cockroaches to aromatic mixtures and medicinal plants often used in households. Attractiveness coefficient was the lowest for cosmetic mixtrures Tutti-fruti and Verbena and Bamboo; other cosmetic aromatizers did not repell this insect (Lilac, Mango) or repelled it poorly (Grapefruit, Amaretto, Pine). Food additives that significantly repelled Sh. lateralis are Apricot, Barberry and Kiwi and lower effects were produced by Biscuit, whereas Vanilla flavouring had no repellent effect. Mixtures for vaping Strawberry pie, Pear, Frozen forest, Irish Cream and Blue Magic exerted strong repellent effects on cockroaches. Low repellent effect on Sh. lateralis were exerted by vaping mixtures Pancakes with Honey, Turkish Tobacco and Grapefruit. No significant effects on the number of cockroaches were exerted by vaping mixtures Vanilla, Club Ice Cream, Blueberry Smoke, Mojito, Chocolate, Apple, Mint and Walnut. Out of the fishing lures, the strongest repellent effects on Sh. lateralis were taken by Blood Worm, Onion and Honey, and weaker effects were exerted by Corn and Vanilla. Imagoes of Sh. lateralis were most significantly repelled by essential oils from jojoba, eucalyptus, daisy, tee tree, Cao Sao Vang balsam, and also fir essential oil. Neither luring nor repellent effects on imagoes of Sh. lateralis were displayed by essential oils from lemon, aloe, peppermint and mandarin. Dry medicinal plants repelled imagoes of Sh. lateralis: inflorescences of Calendula officinalis, leaves of Artemisia absinthium, flowers of Jasminum officinale, leaves of Origanum vulgare, inflorescences of Matricaria chamomilla, inflorescences of Crataegus monogyna, leaves of Mentha x piperita, inflorescences of Achillea millefolium, leaves of Hypericum perforatum, leaves of Aristolochia clematitis and inflorescences of Tanacetum vulgare. No repellent effects on Sh. lateralis were exerted by Chelidonium majus, inflorescences of Tilia cordata and inflorescences of Helichrysum arenarium. Thus, most (40 of 58, or 69.0%) of the tested aromatic substances and medicinal plants repelled synantrophic Turkestan cockroach, while a much smaller share (31.0%) neither significantly lured nor repelled them. No aromatic mixtures attracted Sh. lateralis in our experiment.
      PubDate: 2022-06-04
      DOI: 10.15421/022222
      Issue No: Vol. 13, No. 2 (2022)
  • Multiscale oscillations of the annual course of temperature affect the
           spawning events of rudd (Scardinus erythrophthalmus)

    • Authors: O. M. Kunakh, D. L. Bondarev, N. L. Gubanova, A. V. Domnich, O. V. Zhukov
      Pages: 180 - 188
      Abstract: Identifying climate impacts on ecosystems and their components requires observing time series of sufficient length to ensure adequate statistical power and reasonable coverage of the historical range of variability inherent in the system. The complexity of the hierarchy of climate effects reflected in temporal patterns in time series creates a need to be accurately modeled. The life cycle phenomena of living organisms, including fish spawning, have the character of one-time or time-limited events in time. An approach to finding the relationship between continuous components of time dynamics of environment properties and life cycle events of living organisms was proposed. This approach allowed us to evaluate the role of temperature patterns in the phenology of spawning rudd (Scardinus erythrophthalmus Linnaeus, 1758) in the Dnipro River basin water bodies. The atmospheric temperature time series may be decomposed into the following components: trend, annual cycle, episodic component, harmonic component, extreme events, and noise. Systematically low water temperatures at the beginning of the spawning period were observed in the Protoka River system and the Obukhov floodplain, and systematically elevated temperatures were recorded in the Dnipro River. The annual temperature dynamics was shown to be presented as a composition of oscillatory processes of different scale levels. The sinusoidal trend was previously extracted from the temperature series data. The average annual temperature, amplitude, and phase shift were calculated on the basis of the sinusoidal regression model. The residuals of the sinusoidal trend were processed by means of redundancy analysis with variables derived from symmetric distance-based Moran’s eigenvector maps as explanatory predictors. A set of 104 orthogonal dbMEM variables was extracted from the annual time series. These temporal variables were divided into the broad-, medium-, and fine-scale components. The parameters of temperature dynamics and biotope type are able to explain 51–72% of variability of spawning event. The time of spawning in water bodies corresponds to the time of spawning start: the earlier spawning starts, the earlier it ends. The duration of the spawning season is influenced by the patterns of different scale levels, as well as the amplitude and shift of phases. In this case, the duration of spawning in all water bodies does not differ. Spawning temperature depends on medium- and fine-scale temperature patterns, but does not depend on the characteristics of the sinusoidal annual trend. The annual temperature variation has been shown to be such that it can be decomposed into a sinusoidal trend, patterns of a multiscale nature, and a random fraction. Over the time range studied, the trend of increasing mean annual temperature was not statistically significant for spawning events. The sinusoidal trend explains 78.3–87.6% of the temperature variations and depends on the mean annual temperature, the amplitude of temperature variations during the year, and the earlier or later seasons of the year. Amplitude and phase shift play a role in describing spawning phenology. The residuals of the sinusoidal trend have been explained using dbMEM variables. This variation was decomposed into large-scale, medium-scale, and small-scale components. Winter and spring temperature fluctuations prior to spawning initiation had the greatest effect on spawning. Water temperature determines the lower possible limit for the start of spawning, but the actual start of spawning is determined by the preceding temperature dynamics. The results of the study have implications for understanding the dynamics of fish populations and assessing the influence of environmental conditions on the harmonization of the various components of ecosystems.
      PubDate: 2022-05-04
      DOI: 10.15421/022223
      Issue No: Vol. 13, No. 2 (2022)
  • Metabolism of carbohydrates and activity of the antioxidant system in
           mosses on a post-technogenic salinized territory

    • Authors: N. Y. Kyyak
      Pages: 189 - 196
      Abstract: Adaptive physiological and biochemical reactions of mosses Didymodon rigidulus Hedw., Barbula unguiculata Hedw. and Brachythecium campestre (Müll. Hal.) Schimp. to salt stress have been investigated from the territory of the tailings storage of the Stebnyk Mining and Chemical Enterprise “Polymineral” (Lviv region, Ukraine). The peculiarities of carbohydrate metabolism in mosses under salinity conditions have been studied. The content of soluble carbohydrates and proline, the antioxidant activity, the content of ascorbate and reduced glutathione as well as the activity of enzymes of their metabolism – ascorbate peroxidase and glutathione reductase at the initial stages of the stress (salt shock) and prolonged stress exposure (salt stress) have been evaluated. It has been found that the increase of α-amylase activity, enhancement of the hydrolysis of starch and the increase of the concentration of soluble carbohydrates under salt stress were the reactions of the studied species of mosses. It has been established that there was an increase in the concentration of soluble carbohydrates by 1.2–1.5 times in moss shoots under salinity conditions, compared with plants from the background area (vicinity of Stebnyk). Experimental studies have shown that under salinity conditions sucrose dominates in the pool of soluble carbohydrates (59.0–79.5% of the total sugars content). The sucrose content was 1.5–2.0 times higher in the plants B. unguiculata and D. rigidulus from the highly saline area of the tailings storage. It has been indicated that under stress conditions constitutive adaptive mechanisms are more expressed in resistant moss species, and plants with a lower level of resistance adapt to the stressor, mainly due to induced protective systems. Experimental studies have shown that plants B. unguiculata and D. rigidulus, which are resistant to abiotic stressors, have a high constitutive pool of soluble carbohydrates both at the beginning of the experiment and under prolonged exposure of the salt stress. In the shoots of the sensitive moss B. campestre the stress-induced character of the sugars accumulation has been revealed. The accumulation of proline in mosses cells under salt stress depended on their species characteristics. The stress-induced accumulation of proline can be considered as a part of the bryophytes’ protective system, but this osmolyte does not play a key role in the formation of the mosses’ resistance to salt stress. Obviously, soluble carbohydrates are the main osmolytes in the moss cells. It has been found that resistant moss species have a high constitutive antioxidant status, while in the sensitive moss B. campestre the increase in the antioxidant activity occurred during prolonged salt stress, which may indicate its induced nature. It has been shown that the resistant mosses B. unguiculata and D. rigidulus have 3–4 times higher levels of glutathione and ascorbate content and 1.6–2.5 times higher activity of enzymes of their metabolism – glutathione reductase and ascorbate peroxidase, compared to plants of the less tolerant moss species B. campestre, which provided reduction of the lipid peroxidation process in plasma membranes and decreased the content of TBA-active products under stress.
      PubDate: 2022-05-04
      DOI: 10.15421/022224
      Issue No: Vol. 13, No. 2 (2022)
  • Elucidation of gene action and combining ability for productive tillering
           in spring barley

    • Authors: V. M. Hudzenko, T. P. Polishchuk, A. A. Lysenko, I. V. Fedorenko, M. V. Fedorenko, L. V. Khudolii, V. A. Ishchenko, H. M. Kozelets, A. I. Babenko, S. P. Tanchyk, S. M. Mandrovska
      Pages: 197 - 206
      Abstract: The purpose of the present study is to identify breeding and genetic peculiarities for productive tillering in spring barley genotypes of different origin, purposes of usage and botanical affiliation, as well as to identify effective genetic sources to further improving of the trait. There were created two complete (6 × 6) diallel crossing schemes. Into the Scheme I elite Ukrainian (MIP Tytul and Avhur) and Western European (Datcha, Quench, Gladys, and Beatrix) malting spring barley varieties were involved. Scheme II included awnless covered barley varieties Kozyr and Vitrazh bred at the Plant Production Institute named after V. Y. Yuriev of NAAS of Ukraine, naked barley varieties Condor and CDC Rattan from Canada, as well as awned feed barley variety MIP Myroslav created at MIW and malting barley variety Sebastian from Denmark. For more reliable and informative characterization of barley varieties and their progeny for productive tillering in terms of inheritance, parameters of genetic variation and general combining ability (GCA) statistical analyses of experimental data from different (2019 and 2020) growing seasons were conducted. Accordingly to the indicator of phenotypic dominance all possible modes of inheritance were detected, except for negative dominance in the Scheme I in 2020. The degree of phenotypic dominance significantly varied depending on both varieties involved in crossing schemes and conditions of the years of trials. There was overdominance in loci in both schemes in both years. The other parameters of genetic variation showed significant differences in gene action for productive tillering between crossing Schemes. In Scheme I in both years the dominance was mainly unidirectional and due to dominant effects. In the Scheme II in both years there was multidirectional dominance. In Scheme I compliance with the additive-dominant system was revealed in 2019, but in 2020 there was a strong epistasis. In Scheme II in both years non-allelic interaction was identified. In general, the mode of gene action showed a very complex gene action for productive tillering in barley and a significant role of non-genetic factors in phenotypic manifestation of the trait. Despite this, the level of heritability in the narrow sense in both Schemes pointed to the possibility of the successful selection of individuals with genetically determined increased productive tillering in the splitting generations. In Scheme I the final selection for productive tillering will be more effective in later generations, when dominant alleles become homozygous. In Scheme II it is theoretically possible to select plants with high productive tillering on both recessive and dominant basis. In both schemes the non-allelic interaction should be taken into consideration. Spring barley varieties Beatrix, Datcha, MIP Myroslav and Kozyr can be used as effective genetic sources for involvement in crossings aimed at improving the productive tillering. The results of present study contribute to further development of studies devoted to evaluation of gene action for yield-related traits in spring barley, as well as identification of new genetic sources for plant improvement.
      PubDate: 2022-05-04
      DOI: 10.15421/022225
      Issue No: Vol. 13, No. 2 (2022)
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