Authors:Mansheng Luo, Yanmei Zeng, Xiaoling Deng Pages: 1 - 7 Abstract: Till now, the medicines approved for acute myeloid leukemia with internal tandem duplication mutation of FMS-like tyrosine kinase 3 (FLT3-ITD) display not ideal efficacy. This study aimed to evaluate the effects of 15,16-dihydrotanshinone I on FLT3-ITD acute myeloid leukemia cells. The inhibitory effect of this compound against MV4-11 was determined using CCK-8 assay. Western blotting detecting caspase-3, PARP, and annexin V-APC/7-AAD was carried out. Activation of FLT3, STAT5, and Mcl-1 expression was analyzed by western blotting. The results showed that MV4-11 was sensitive toward dihydrotanshinone I in a dose-dependent manner (p<0.05). MV4-11 apoptosis was induced notably after dihydrotanshinone I treatment. Western blotting revealed suppressed activation of FLT3, STAT5 and decreased Mcl-1 (p<0.05). This study suggests that dihydrotanshinone I inhibits MV4-11 proliferation by apoptosis via antagonizing FLT3-ITD/STAT5/Mcl-1 path-way, which might provide a novel therapy for acute myeloid leukemia. PubDate: 2023-01-13 DOI: 10.3329/bjp.v18i1.62376 Issue No:Vol. 18, No. 1 (2023)
Authors:Yohan Han, Young Seok Eom, Fahad Hassan Shah, Song Ja Kim Pages: 8 - 16 Abstract: The role of cell morphological changes in colchicine-inhibited tubulin polymerization of rabbit articular chondrocytes and their involvement in dedifferentiation were investigated. Colchicine treatment resulted in the dedifferentiation of chondrocytes, which was supported by the loss of type II collagen expression and proteoglycan production. Inhibition of tubulin de-polymerization with paclitaxel rescued colchicine-caused dedifferentiation and tubulin polymerization. Additionally, colchicine stimulated β-catenin overexpression, which is characterized by the accumulation of β-catenin into the cytosol determined by immunofluorescence staining. Inhibition of the β-catenin-mediated pathway by siR β-catenin recovered colchicine-caused the suppression of type II collagen expression in the chondrocytes. Treatment with colchicine also induced inflammation, as determined by the increased expression level of cyclooxygenase-2 and decreased IκB-α expression level by western blot analysis. Modulating the expression levels of pIκBα and IκBα via BMS 345541, was able to modulate colchicine-induced inflammatory effect. PubDate: 2023-01-13 DOI: 10.3329/bjp.v18i1.62725 Issue No:Vol. 18, No. 1 (2023)
Authors:Ranjitha Dhevi V. Sundar, Sathiavelu Arunachalam Pages: 17 - 23 Abstract: This study aimed to explore the in vitro antibacterial potential of endophytic fungus isolated from the roots of Polianthes tuberosa against multidrug-resistant pathogens. Fungal isolates were screened for their antibacterial activities by disc diffusion, agar plug diffusion, MIC, and MBC method. GC-MS was carried out to determine the crude extract's chemical profile. The highest antagonistic effect in the disc diffusion method was seen in the ethyl acetate extract of PTR3, with an inhibitory zone ranging from 14-18 mm. Therefore, the potent isolate was identified as Daldinia eschscholtzii. The maximum inhibition of 18 mm and 20 mm was observed against MRSA (ATCC 43300) (ATCC 700699), followed by VRE (14 mm). The MIC and MBC were between 3.12-25 μg/mL and 6.25-50 μg/mL, respectively. 1,2-Benzene dicarboxylic acid and diheptyl ester were the primary chemical constituents present in the extract. These findings confirm that D. eschscholtzii PTR3 crude extract shows antibacterial activity. PubDate: 2023-01-13 DOI: 10.3329/bjp.v18i1.62914 Issue No:Vol. 18, No. 1 (2023)
Authors:Polly Ho-Ting Shiu, Chengwen Zheng, Panthakarn Rangsinth, Wen Wang, Jingjing Li, Renkai Li, George Pak-Heng Leung Pages: 24 - 32 Abstract: The objectives of the present study were to evaluate the antioxidant and anti-inflammatory effects of gallic acid, a naturally occurring triphenolic compound, on HaCaT keratinocytes and study its mechanisms of action. The results showed that gallic acid at concentrations lower than 30 µM was non-toxic to HaCaT cells, reduced the intracellular level of reactive oxygen species, and suppressed the release of chemokines interleukin-8 and monocyte chemoattractant protein-1 in tumor necrosis factor-α- and interferon-γ-stimulated HaCaT keratinocytes. In addition, gallic acid reduced the total protein expression of NF-κB and inhibited the phosphorylation of ERK1/2, p38 MAPK, and Akt in stimulated HaCaT keratinocytes. In conclusion, our study revealed that gallic acid exhibited antioxidant and anti-inflammatory effects on keratinocytes, probably through the inhibition of MAPK-, NF-κB-, and Akt-dependent signaling pathways. PubDate: 2023-01-13 DOI: 10.3329/bjp.v18i1.63052 Issue No:Vol. 18, No. 1 (2023)
Authors:Jawhar O. Interino, Nancy C. Alombro, Peter Jan D. de Vera Pages: 33 - 35 Abstract: No abstract PubDate: 2023-01-13 DOI: 10.3329/bjp.v18i1.62627 Issue No:Vol. 18, No. 1 (2023)
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