|
Biodegradation
Journal Prestige (SJR): 0.876  Citation Impact (citeScore): 2 Number of Followers: 2  Hybrid journal (It can contain Open Access articles)ISSN (Print) 1572-9729 - ISSN (Online) 0923-9820 Published by Springer-Verlag  [2467 journals]
|
- Metaproteomic and gene expression analysis of interspecies interactions in
a PAH-degrading synthetic microbial consortium constructed with the key
microbes of a natural consortium-
Free pre-print version: Loading...
Abstract: Abstract Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
PubDate: 2023-01-04
-
- Impacts of co-contaminants and dilution on perchlorate biodegradation
using various carbon sources-
Free pre-print version: Loading...
Abstract: This research investigates the biodegradation of perchlorate in the presence of the co-contaminants nitrate and chlorate using soluble and slow-release carbon sources. In addition, the impact of bio-augmentation and dilution, which results in lower total dissolved salts (TDS) and contaminant levels, is examined. Laboratory microcosms were conducted using actual groundwater and soils from a contaminated aquifer. The results revealed that both soluble and slow-release carbon sources support biodegradation of contaminants in the sequence nitrate > chlorate > perchlorate. Degradation rates, including and excluding lag times, revealed that the overall impact of the presence of co-contaminants depends on degradation kinetics and the relative concentrations of the contaminants. When the lag time caused by the presence of the co-contaminants is considered, the degradation rates for chlorate and perchlorate were two to three times slower. The results also show that dilution causes lower initial contaminant concentrations, and consequently, slower degradation rates, which is not desirable. On the other hand, the dilution resulting from the injection of amendments to support remediation promotes desirably lower salinity levels. However, the salinity associated with the presence of sulfate does not inhibit biodegradation. The naturally occurring bacteria were able to support the degradation of all contaminants. Bio-augmentation was effective only in diluted microcosms. Proteobacteria and Firmicutes were the dominant phyla identified in the microcosms. Graphical abstract
PubDate: 2023-01-04
-
- Isolation and identification of the molybdenum-resistant strain Raoultella
ornithinolytica A1 and its effect on MoO42− in the environment-
Free pre-print version: Loading...
Abstract: The mining and leakage of molybdenum (Mo) can cause environmental contamination which has not been realized until recently. Bacteria that can mitigate Mo-contamination was enriched and isolated. The low temperature and different pH conditions were considered to analysis its feasibility in Northern China which suffers from a long time of low temperatures every year. The result showed that the removal rate of MoO42− by Raoultella ornithinolytica A1 reached 30.46% at 25 °C and pH 7.0 in Luria–Bertani medium (LB). Meanwhile, A1 also showed some efficiency in the reduction of MoO42− in low phosphate molybdate medium (LPM), which reached optimum at the MoO42− concentration of 10 mM. The results of FTIR indicated that the cell wall performed an essential role in the MoO42− removal process, which was illustrated by the distribution of Mo in A1 (Mo bound to cell wall accounted for 92.29% of the total MoO42− removed). In addition, low temperature (10 °C) effect the removal rate of MoO42− by − 8.38 to 11.66%, indicating the potential for the in-situ microbial remediation of Mo-contaminated environments in low temperature areas. Graphical abstract
PubDate: 2023-01-04
-
- Biodegradation of organic compounds in the coal gangue by Bacillus sp.
into humic acid-
Free pre-print version: Loading...
Abstract: Abstract Coal gangue (CG), one of the world’s largest industrial solid wastes produced during coal mining, is extremely difficult to be used owing to its combined contents of clay minerals and organic macromolecules. This study explored a novel process of degrading the harmful organic compounds in the CG into humic acid using a biological method characterized by scanning electron microscope–energy dispersive spectrometer, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and elemental analyzer. The results reveal that adding selected Bacillus sp. to the CG for 40 days can increase the humic acid content by ~ 17 times, reaching 17338.17 mg/kg, which is also the best level for promoting plant growth. FTIR and XPS spectra show that the organic compounds in the CG transforms primarily from C=C to C=O, COOH, and O–H groups, indicating that the organic compounds are gradually oxidized and activated, improving the humic acid concentration of soil. In addition, Bacillus sp. decreases pH and benzo[a]pyrene contents, and increases the content of available nutrients. After microbial degradation, coal gangue can be turned into ecological restoration materials.
PubDate: 2023-01-03
-
- Biodegradation of chemicals tested in mixtures and individually: mixture
effects on biodegradation kinetics and microbial composition-
Free pre-print version: Loading...
Abstract: Biodegradation in the aquatic environment occurs in the presence of many chemicals, while standard simulation biodegradation tests are conducted with single chemicals. This study aimed to investigate the effect of the presence of additional chemicals on (1) biodegradation kinetics of individual chemicals and (2) the microbial composition in test systems. Parallel mixture and single substance experiments were conducted for 9 chemicals (phenethyl benzoate, oxacycloheptadec-10-en-2-one, α-ionone, methyl 2-naphthyl ether, decan-5-olide, octan-2-one, 2′-acetonaphthanone, methyl N-methylanthranilate, (+)-menthone) using inoculum from a Danish stream. Biotic and abiotic test systems were incubated at 12 °C for 1–30 days. Primary biodegradation kinetics were then determined from biotic/abiotic peak area ratios using SPME GC/MS analysis. The effect of the mixture on biodegradation varied with test chemical and was more pronounced for chemicals with lag-phases above 14 days: two chemicals degraded in the mixture but not when tested alone (i.e., positive mixture effect), and two degraded when tested alone but not in the mixture (i.e., negative mixture effect). Microbial composition (16S rRNA gene amplicon sequencing) was highly affected by 14 days incubation and the presence of the mixture (significant carbon source), but less by single chemicals (low carbon source). Growth on chemical mixtures resulted in consistent proliferation of Pseudomonas and Malikia, while specific chemicals increased the abundance of putative degraders belonging to Novosphingobium and Zoogloea. The chemical and microbiological results support (1) that simulation biodegradation kinetics should be determined in mixtures at low environmentally relevant concentrations and (2) that degradation times beyond some weeks are associated with more uncertainty. Graphical abstract
PubDate: 2023-01-03
-
- Enhanced bioremediation of oil-contaminated soil in a slurry bioreactor by
H2O2-stimulation of oil-degrading/biosurfactant-generating bacteria:
performance optimization and bacterial metagenomics-
Free pre-print version: Loading...
Abstract: Abstract Oil-contaminated soil is the main challenge for oil-rich countries, and this study aimed to investigate the performance of the H2O2-stimulated slurry bioreactor for the bioremediation of real oil-contaminated soil. The effect of biomass concentration, soil to water (S/W) ratio, slurry temperature, pH, and H2O2 concentration were optimized for the removal of total petroleum hydrocarbons (TPH) from oil-contaminated soil. TPH removal efficiency, biosurfactants production, and peroxidase and dehydrogenase activities were measured. The optimum conditions for the complete biodegradation of 32 \({\text{g}}_{{{\text{TPH}}}} /{\text{kg}}_{{{\text{soil}}}}\) in the slurry bioreactor during 6 days were biomass of 2250 mg/L, S/W ratio of 20%, the temperature of 30 °C, pH of 7, and an H2O2 concentration of 120 mg/L. The highest peroxidase, dehydrogenase, surfactin, and rhamnolipid formation were also obtained under optimum conditions. The results pointed out that complete biodegradation of 32 g/kg of TPH in oil-contaminated soil at a short reaction time of 6 days is achievable in the developed process operated under optimum conditions. The GC/FID analysis of solid and liquid phases showed that the bioprocess completely biodegraded the different TPH fractions. H2O2 efficiently stimulated the biosurfactant-generating bacteria to produce peroxidase and thereby accelerating the bioremediation rate. Accordingly, an H2O2-mediated slurry bioreactor inoculated with biosurfactant/peroxidase-generating bacteria is a promising technique for cleaning up oil-contaminated soils.
PubDate: 2023-01-02
-
- Goethite and riboflavin synergistically enhance Cr(VI) reduction by
Shewanella oneidensis MR-1-
Free pre-print version: Loading...
Abstract: Abstract Bioreduction of Cr(VI) is cost-effective and environmentally friendly, however, the slow bioreduction rate limits its application. In this study, the potential synergistic enhancement of Cr(VI) bioreduction by shewanella oneidensis MR-1 (S. oneidensis) with goethite and riboflavin (RF) was investigated. The results showed that the S. oneidensis reaction system reduce 29.2% of 20 mg/L Cr(VI) after 42 h reaction, while the S. oneidensis/goethite/RF reaction system increased the Cr(VI) reduction rate to 87.74%. RF as an efficient electron shuttle and Fe(II) from goethite bioreduction were identified as the crucial components in Cr(VI) reduction. XPS analysis showed that the final precipitates of Cr(VI) reduction were Cr(CH3C(O)CHC(O)CH3)3 and Cr2O3 and adhered to the bacterial cell surface. In this process, the microbial surface functional groups such as hydroxyl and carboxyl groups participated in the adsorption and reduction of Cr(VI). Meanwhile, an increase in cytochrome c led to an increase in electron transfer system activity (ETSA), causing a significant enhancement in extracellular electron transfer efficiency. This study provides insight into the mechanism of Cr(VI) reduction in a complex environment where microorganisms, iron minerals and RF coexist, and the synergistic treatment method of Fe(III) minerals and RF has great potential application for Cr(VI) detoxification in aqueous environment.
PubDate: 2023-01-02
-
- Bioremediation of heavy oily sludge: a microcosms study
-
Free pre-print version: Loading...
Abstract: Abstract Oily sludge is a residue from the petroleum industry composed of a mixture of sand, water, metals, and high content of hydrocarbons (HCs). The heavy oily sludge used in this study originated from Colombian crude oil with high density and low American Petroleum Institute (API) gravity. The residual waste from heavy oil processing was subject to thermal and centrifugal extraction, resulting in heavy oily sludge with very high density and viscosity. Biodegradation of the total petroleum hydrocarbons (TPH) was tested in microcosms using several bioremediation approaches, including: biostimulation with bulking agents and nutrients, the surfactant Tween 80, and bioaugmentation. Select HC degrading bacteria were isolated based on their ability to grow and produce clear zones on different HCs. Degradation of TPH in the microcosms was monitored gravimetrically and with gas chromatography (GC). The TPH removal in all treatments ranged between 2 and 67%, regardless of the addition of microbial consortiums, amendments, or surfactants within the tested parameters. The results of this study demonstrated that bioremediation of heavy oily sludge presents greater challenges to achieve regulatory requirements. Additional physicochemical treatments analysis to remediate this recalcitrant material may be required to achieve a desirable degradation rate.
PubDate: 2022-12-04
-
- Aerobic degradation of bisphenol A by Pseudomonas sp. LM-1: characteristic
and pathway-
Free pre-print version: Loading...
Abstract: Abstract Bisphenol A (BPA) has been widely used in the manufacture of polymeric materials. BPA is regarded as an endocrine disrupting chemical, posing a great threat to the public health. In this study, a bacterial strain LM-1, capable of utilizing BPA as the sole carbon and energy source under aerobic conditions, was originally isolated from an activated sludge sample. The isolate was identified as Pseudomonas sp. based on 16S rRNA gene sequence analysis. Strain LM-1 was able to completely degrade 25–100 mg/L BPA within 14–24 h, and it also exhibited high capacity for BPA degradation at a range of pH (6.0–8.0). (NH4)2SO4 and NH4NO3 were the suitable nitrogen sources for its growth and BPA biodegradation, and the BPA degradation could be accelerated when exogenous carbon sources were introduced as the co-substrates. Metal ions such as Zn2+, Cu2+, and Ni2+ could considerably suppress the growth of strain LM-1 and BPA degradation. According to the analysis of liquid chromatography coupled to Q-Exactive high resolution mass spectrometry, hydroquinone, p-hydroxybenzaldehyde, and p-hydroxybenzoate were the predominate metabolites in the BPA biodegradation and the degradation pathways were proposed. This study is important for assessment of the fate of BPA in engineered and natural systems and possibly for designing bioremediation strategies.
PubDate: 2022-11-19
DOI: 10.1007/s10532-022-10003-4
-
- Role of pine needle biochar in operation and stability of anaerobic
processes-
Free pre-print version: Loading...
Abstract: Utility of biochar addition in anaerobic processes for promoting direct interspecies electron transfer (DIET) is demonstrated in this research. Biochar produced from pyrolysis of pine needle forest residue was used as conductive material for DIET. Three CSTRs were operated in parallel with and without biochar addition in fed-batch mode. Reactor without biochar which represented indirect interspecies electron transfer (IIET) exhibited wide variation in pH and VFA and took longer period during startup. All the rectors were operated at steady state with an OLR ranging from 0.5 to 1.75 kg-COD/m3.d. As OLR increased, performance of reactor without biochar resulted in rapid pH drop and increase in VFA, leading to its eventual failure at OLR of 1.75 kg-COD/m3.d. As against to this, performance of reactors with biochar remained robust and relatively unaffected at higher OLR values. Daily VFA accumulation from fed-batch mode always remained highest in reactor without biochar. Graphical
PubDate: 2022-11-18
DOI: 10.1007/s10532-022-10004-3
-
- Optimized culture conditions facilitate the estrone biodegradation ability
and laccase activity of Spirulina CPCC-695-
Free pre-print version: Loading...
Abstract: Abstract Endocrine disrupting compounds (EDCs) are emerging contaminants that persist and contaminate the environment. They mimic hormones, block hormones, or modulate their synthesis, metabolism, transport, and action, affecting living organisms and their progeny. Steroid hormones from exogenous sources like water bodies are important EDCs. Their biodegradation is an urgent global need. The present study is a preliminary work to maximize the estrone degradation potential of Spirulina CPCC-695 and study the effect of optimized conditions on its laccase activity. It was observed that the exponential phase culture at pH 10.0, 30 ℃, and 200 rpm of agitation speed resulted in the maximum growth, estrone degradation efficiency (93.12%), and highest laccase activity (74%) of Spirulina CPCC-695.
PubDate: 2022-11-17
DOI: 10.1007/s10532-022-10005-2
-
- Evaluation of aerobic biodegradation of phenanthrene using Pseudomonas
turukhanskensis: an optimized study-
Free pre-print version: Loading...
Abstract: The ability of Pseudomonas turukhanskensis GEEL-01 to degrade the phenanthrene (PHE) was optimized by response surface methodology (RSM). Three factors as independent variables (including temperature, pH, and inoculum) were studied at 600 mg/L PHE where the highest growth of P. turukhanskensis GEEL-01 was observed. The optimum operating conditions were evaluated through the fit summary analysis, model summary statistics, fit statistics, ANOVA analysis, and model graphs. The degradation of PHE was monitored by high-performance liquid chromatography (HPLC) and the metabolites were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the correlation among independent variables with experimental and predicted responses was significant (p < 0.0001). The optimal temperature, pH, and inoculum were 30 ℃, 8, and 6 mL respectively. The HPLC peaks exhibited a reduction in PHE concentration from 600 mg/L to 4.97 mg/L with 99% degradation efficiency. The GC-MS peaks indicated that the major end products of PHE degradation were 1-Hydroxy-2-naphthoic acid, salicylic acid, phthalic acid, and catechol. This study demonstrated that the optimized parameters by RSM for P. turukhanskensis GEEL-01 could degrade PHE by phthalic and salicylic acid pathways. Graphical
PubDate: 2022-11-12
DOI: 10.1007/s10532-022-10002-5
-
- Myco-degradation of microplastics: an account of identified pathways and
analytical methods for their determination-
Free pre-print version: Loading...
Abstract: Microplastics (MPs) have sparked widespread concern due to their non-degradable and persistent nature in ecosystems. Long-term exposure to microplastics can cause chronic toxicity, including impaired reproduction and malnutrition, threatening biota and humans. Microplastics can also cause ingestion, choking, and entanglement in aquatic populations. Thus, it is crucial to establish remarkably effective approaches to diminish MPs from the environment. In this regard, using fungi for microplastic degradation is beneficial owing to its diverse nature and effective enzymatic system. Extracellular and intracellular enzymes in fungi degrade the plastic polymers into monomers and produce carbon dioxide and water under aerobic conditions whereas methane under anaerobic conditions. Further, fungi also secrete hydrophobins (surface proteins) which serve as a crucial aid in the bioremediation process by promoting substrate mobility and bioavailability. Therefore, the present review provides insight into the mechanism and general pathway of fungal-mediated microplastic degradation. Additionally, analytical techniques for the monitoring of MPs degradation along with the roadblocks and future perspectives have also been discussed. However, more research is required to fully perceive the underlying process of microplastic biodegradation in the environment using fungus, to establish an effective and sustainable practice for its management. Graphical abstract
PubDate: 2022-10-13
DOI: 10.1007/s10532-022-10001-6
-
- Applying the 15N labelling technique to material derived from a landfill
simulation experiment to understand nitrogen cycle processes under aerobic
and anaerobic conditions-
Free pre-print version: Loading...
Abstract: Abstract Reactive nitrogen (N) species, such as ammonium (NH4+), nitrate (NO3) and gaseous nitrous oxide (N2O), are released into the environment during the degradation of municipal solid waste (MSW), causing persistent environmental problems. Landfill remediation measures, such as in-situ aeration, may accelerate the degradation of organic compounds and reduce the discharge of ammonium via leachate. Nonetheless, the actual amount of N in the waste material remains relatively constant and a coherent explanation for the decline in leachate ammonium concentrations is still lacking. Hence, the present study aimed to elucidate the dynamics of N and its transformation processes during waste degradation. To this end, the gross rates of organic N mineralization and nitrification were measured using 15N pool dilution in waste material derived from a landfill simulation reactor (LSR) experiment. The results revealed a high potential for N mineralization and nitrification, the latter of which declined with the diminishing amount of extractable ammonium (after aeration). The analysis of the concentration and isotopic composition of N2O formed confirmed incomplete denitrification as the main source for N2O. Moreover, the natural abundance of 15N was investigated in various waste N pools to verify the conclusions drawn from the 15N tracing experiment. δ15N values of total waste N increased during aeration, indicating that nitrification is the major driver for N losses from aerated waste. The application of stable isotopes thereby allowed unprecedented insights into the complex N dynamics in decomposing landfill waste, of their response to aeration and their effect on hydrological versus gaseous loss pathways.
PubDate: 2022-10-11
DOI: 10.1007/s10532-022-10000-7
-
- Enhanced biodegradation of oil-contaminated soil oil in shale gas
exploitation by biochar immobilization-
Free pre-print version: Loading...
Abstract: The enhanced biodegradation of oil-contaminated soil by fixing microorganisms with corn cob biochar was investigated. It was found that the components of oil in the test soil were mainly straight-chain alkanes and branched alkanes. When using corn cob biochar as a carrier to immobilize microorganisms, the best particle size of corn cob biochar as an immobilization carrier was 0.08 mm, and the best immobilization time was 18 h. SEM analysis confirmed that the microorganisms were immobilized on the corn cob biochar. Immobilized microorganisms exhibited high biodegradability under stress to high concentrations of petroleum pollutants, heavy metals, and organic pollutants. Infrared spectroscopy analysis showed that oxygen-containing groups such as hydroxyl, carboxyl, and methoxy on the surface of biochar were involved in the complexation of heavy metals. The mechanism of immobilization promoted microbial degradation of oil contamination was explained by gas chromatography mass. First, alkanes and aromatics were adsorbed by corn cob biochar and passed to immobilized microorganisms to promote their degradation. Their bioavailability increased, especially for aromatics. Second, biochar provided a more suitable environment for microorganisms to degrade. Third, the conversion of ketones to acids was accelerated during the biodegradation of alkanes, and the biodegradation of alkanes was accelerated by immobilization. The biodegradable efficiency of oil by immobilized microorganisms in soil was 70.10% within 60 days, 28.80% higher than that of free microorganisms. The degradation of immobilized microorganisms was highly correlated with the activities of catalase, urease, and polyphenol oxidase. Graphical abstract
PubDate: 2022-10-10
DOI: 10.1007/s10532-022-09999-6
-
- Biodegradation of rubber in cultures of Rhodococcus rhodochrous and by its
enzyme latex clearing protein-
Free pre-print version: Loading...
Abstract: Abstract The biodegradation of rubber materials is considered as a sustainable recycling alternative, highlighting the use of microorganisms and enzymes in oxidative processes of natural rubber. Currently, the main challenge is the treatment of rubber materials such as waste tyres, where the mixture of rubber polymers with different additives and the cross-linked structure obtained due to the vulcanisation process positions them as highly persistent materials. This study characterises the degradation of different rubber-containing substrates in in vivo and in vitro processes using the bacterium Rhodococcus rhodochrous and the oxygenase latex clearing protein (Lcp) from the same strain. For the first time, the degradation of polyisoprene particles in liquid cultures of R. rhodochrous was analysed, obtaining up to 19.32% mass loss of the polymer when using it as the only carbon source. Scanning electron microscopy analysis demonstrated surface alteration of pure polyisoprene and vulcanised rubber particles after 2 weeks of incubation. The enzyme LcpRR was produced in bioreactors under rhamnose induction and its activity characterised in oxygen consumption assays at different enzyme concentrations. A maximum consumption of 28.38 µmolO2/min was obtained by adding 100 µg/mL LcpRR to a 2% (v/v) latex emulsion as substrate. The bioconversion of natural rubber into reaction degradation products or oligoisoprenoids was calculated to be 32.54%. Furthermore, the mass distribution of the oligoisoprenoids was analysed by liquid chromatography coupled to mass spectrometry (LC–MS) and 17 degradation products, ranging from C20 to C100 oligoisoprenoids, were identified. The multi-enzymatic degradation capacity of R. rhodochrous positions it as a model microorganism in complex degradation processes such as in the case of tyre waste.
PubDate: 2022-10-05
DOI: 10.1007/s10532-022-09998-7
-
- Bacillus pumilus proteome changes in response to
2,4,6-trinitrotoluene-induced stress-
Free pre-print version: Loading...
Abstract: Abstract 2,4,6-Trinitrotoluene (TNT) is the most widely used nitroaromatic compound and is highly resistant to degradation. Most aerobic microorganisms reduce TNT to amino derivatives via formation of nitroso- and hydroxylamine intermediates. Although pathways of TNT degradation are well studied, proteomic analysis of TNT-degrading bacteria was done only for some individual Gram-negative strains. Here, we isolated a Gram-positive strain from TNT-contaminated soil, identified it as Bacillus pumilus using 16S rRNA sequencing, analyzed its growth, the level of TNT transformation, ROS production, and revealed for the first time the bacillary proteome changes at toxic concentration of TNT. The transformation of TNT at all studied concentrations (20–200 mg/L) followed the path of nitro groups reduction with the formation of 4-amino-2,6-dinitrotoluene. Hydrogen peroxide production was detected during TNT transformation. Comparative proteomic analysis of B. pumilus showed that TNT (200 mg/L) inhibited expression of 46 and induced expression of 24 proteins. Among TNT upregulated proteins are those which are responsible for the reductive pathway of xenobiotic transformation, removal of oxidative stress, DNA repair, degradation of RNA and cellular proteins. The production of ribosomal proteins, some important metabolic proteins and proteins involved in cell division are downregulated by this xenobiotic.
PubDate: 2022-08-18
DOI: 10.1007/s10532-022-09997-8
-
- Efficient bioremediation of PAHs-contaminated soils by a methylotrophic
enrichment culture-
Free pre-print version: Loading...
Abstract: Abstract Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. While screening for pyrene-degrading bacteria from a former manufactured gas plant soil (MGPS), the mixed enrichment culture was found to be more efficient in PAHs biodegradation than the culturable pure strains. Interestingly, analysis of 16S rRNA sequences revealed that the culture was dominated by a previously uncultured member of the family Rhizobiaceae. The culture utilized C1 and other methylotrophic substrates, including dimethylformamide (DMF), which was used as a solvent for supplementing the culture medium with PAHs. In the liquid medium, the culture rapidly degraded phenanthrene, pyrene, and the carcinogenic benzo(a)pyrene (BaP), when provided as the sole carbon source or with DMF as a co-substrate. The efficiency of the culture in the bioremediation of PAHs from the MGPS and a laboratory waste soil (LWS) was evaluated in bench-scale slurry systems. After 28 days, 80% of Σ16 PAHs were efficiently removed from the inoculated MGPS. Notably, the bioaugmentation achieved 90% removal of four-ringed and 60% of highly recalcitrant five- and six-ringed PAHs from the MGPS. Likewise, almost all phenanthrene, pyrene, and 65% BaP were removed from the bioaugmented LWS. This study highlights the application of the methylotrophic enrichment culture dominated by an uncultured bacterium for the efficient bioremediation of PAHs.
PubDate: 2022-08-17
DOI: 10.1007/s10532-022-09996-9
-
- Composite hydrolytic acidification - aerobic MBBR process for treating
traditional Chinese medicine wastewater-
Free pre-print version: Loading...
Abstract: Traditional Chinese medicine (TCM) wastewater is characterized by high organic content, unstable water quality and quantity and low biodegradability. In this paper, the hydrolytic acidification reactor—aerobic moving bed biofilm (MBBR) process was used to degrade TCM wastewater. Besides, a small pilot study was conducted. The appropriate operating parameters: hydraulic retention time (HRT) of the hydrolytic reactor was 16 h, HRT of MBBR was 30 h, dissolved oxygen of MBBR was 6 mg/L, sludge return ratio of MBBR was 100%. The hydrolytic reactor was started for 25 days. MBBR was run in series with the hydrolytic reactor after 24 days of separate operation. The start-up of the composite reactor was completed after another 26 days. The average removal efficiencies of chemical oxygen demand and ammonia nitrogen were 92% and 70%. The hydrolytic reactor was effective in decomposing macromolecules and MBBR had a strong ability to degrade pollutants through the excitation-emission-matrix spectra. The evolution pattern of the dominant bacterial genera and the surface morphology of sludge were studied by scanning electron microscopy and high-throughput sequencing analysis. It could be seen that the surface morphology of the biological filler was suitable for the growth and reproduction of microorganisms. Graphical abstract
PubDate: 2022-08-10
DOI: 10.1007/s10532-022-09995-w
-
- Degradation characteristics of crude oil by a consortium of bacteria in
the existence of chlorophenol-
Free pre-print version: Loading...
Abstract: In order to enhance the degradation effect of microorganisms on crude oil in the existence of chlorophenol compounds, oil-degrading bacteria C4 (Alcaligenes faecails), C5 (Bacillus sp.) and 2,4-dichlorophenol (2,4-DCP) degrading bacteria L3 (Bacillus marisflavi), L4 (Bacillus aquimaris) were isolated to construct a highly efficient consortium named (C4C5 + L3L4). When the compound bacteria agent combination by VC4: VC5: VL3: VL4 = 1:2:2:1, the crude oil degradation efficiency of 7 days was stable at 50.63% ~ 55.43% under different conditions. Degradation mechanism was analyzed by FTIR, GC–MS and IC technology and the following conclusions showed that in the system of adding consortium (C4C5 + L3L4), the heavy components were converted into saturated and unsaturated components. The bacterial consortium could first degrade medium and long chain alkanes into short chain hydrocarbons and then further degrade. And the dechlorination efficiency of 2,4-DCP in the degradation system reached 73.83%. The results suggested that the potential applicability and effectiveness of the selected bacteria consortium for the remediation of oil-contaminated water or soil with the existence of chlorophenol compound. Graphical abstract
PubDate: 2022-06-21
DOI: 10.1007/s10532-022-09992-z
-
