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Nature Protocols
Number of Followers: 72

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ISSN (Print) 1750-2799 - ISSN (Online) 1750-2799
Published by NPG

[138 journals]

A puromycin-dependent activity-based sensing probe for histochemical
staining of hydrogen peroxide in cells and animal tissues
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  Nature Protocols, Published online: 13 May 2022; doi:10.1038/s41596-022-00694-7
  This protocol details the synthesis and use of Peroxymycin-1, an activity-based histochemical probe for hydrogen peroxide detection in fixed cell and mouse tissue samples.Nature Protocols, Published online: 2022-05-13; doi:10.1038/s41596-022-00694-72022-05-13
  DOI: 10.1038/s41596-022-00694-7
Patient-derived and mouse endo-ectocervical organoid generation, genetic
manipulation and applications to model infection
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  Nature Protocols, Published online: 11 May 2022; doi:10.1038/s41596-022-00695-6
  Mouse or human organoids are generated from two cervical regions: columnar epithelium-lined endocervix and stratified squamous epithelium-lined ectocervix. Genetic manipulation for modeling cancers and organoid infections is also described.Nature Protocols, Published online: 2022-05-11; doi:10.1038/s41596-022-00695-62022-05-11
  DOI: 10.1038/s41596-022-00695-6
Hyperpolarized water as universal sensitivity booster in biomolecular NMR
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  Nature Protocols, Published online: 11 May 2022; doi:10.1038/s41596-022-00693-8
  In this work, ‘hyperpolarized water’ produced by cryogenic dynamic nuclear polarization is used to boost signal intensities in NMR of proteins and nucleic acids. This allows NMR at lower concentrations and detection of folding intermediates.Nature Protocols, Published online: 2022-05-11; doi:10.1038/s41596-022-00693-82022-05-11
  DOI: 10.1038/s41596-022-00693-8
Chromatin accessibility profiling by ATAC-seq
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  Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00692-9
  A protocol for generating chromatin accessibility profiles from a broad variety of cell and tissue types, including a step-by-step workflow for library preparation and guidelines for data processing and downstream analysis.Nature Protocols, Published online: 2022-04-27; doi:10.1038/s41596-022-00692-92022-04-27
  DOI: 10.1038/s41596-022-00692-9
All-optical interrogation of neural circuits in behaving mice
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  Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00691-w
  This protocol describes all-optical interrogation experiments in awake, behaving mice, demonstrating the utility of this strategy in three brain areas—barrel cortex, visual cortex and hippocampus—by using different experimental setups.Nature Protocols, Published online: 2022-04-27; doi:10.1038/s41596-022-00691-w2022-04-27
  DOI: 10.1038/s41596-022-00691-w
Sister chromatid–sensitive Hi-C to map the conformation of
replicated genomes
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  Nature Protocols, Published online: 27 April 2022; doi:10.1038/s41596-022-00687-6
  This protocol combines in situ chromosome conformation capture with labeling of nascent DNA with the synthetic nucleoside 4-thio-thymidine to generate genome-wide contact probability maps within and across sister chromatids in mammalian cells.Nature Protocols, Published online: 2022-04-27; doi:10.1038/s41596-022-00687-62022-04-27
  DOI: 10.1038/s41596-022-00687-6
l-RNA+aptamers+by+rG4-SELEX&rft.title=Nature+Protocols&rft.issn=1750-2799&rft.date=2022&rft.volume=&rft_id=info:doi/10.1038/s41596-022-00679-6">Development of RNA G-quadruplex (rG4)-targeting l-RNA aptamers by
rG4-SELEX
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  Nature Protocols, Published online: 20 April 2022; doi:10.1038/s41596-022-00679-6
  This Protocol Extension (to an existing SELEX Nature Protocol) introduces RNA G-quadruplex (rG4)-SELEX, a method that generates novel l-RNA aptamers to target rG4 structures that can be applied to inhibit G-quadruplex-mediated interactions.Nature Protocols, Published online: 2022-04-20; doi:10.1038/s41596-022-00679-62022-04-20
  DOI: 10.1038/s41596-022-00679-6
Subretinal injection in mice to study retinal physiology and disease
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  Nature Protocols, Published online: 13 April 2022; doi:10.1038/s41596-022-00689-4
  Mice undergo subretinal injection, with successful injection confirmed by follow-up examination of the retina and the retinal pigmented epithelium.Nature Protocols, Published online: 2022-04-13; doi:10.1038/s41596-022-00689-42022-04-13
  DOI: 10.1038/s41596-022-00689-4