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Advances in Oceanography and Limnology
Number of Followers: 15

This is an Open Access Journal

Open Access journal
ISSN (Print) 1947-5721 - ISSN (Online) 1947-573X
Published by PAGEPress

[51 journals]

Alpine freshwater fish biodiversity assessment: an inter-calibration test
for metabarcoding method set up
- Authors: Giulia Riccioni, Isabelle Domaizon, Andrea Gandolfi, Massimo Pindo, Adriano Boscaini, Marine Vautier, Hans Rund, Peter Hufnagl, Stefanie Dobrovolny, Valentin Vasselon, Jonas Bylemans, Cuong Q. Tang, Josef Wanzenböck
  Abstract: The analysis of environmental DNA (eDNA) by high throughput sequencing (HTS) is proving to be a promising tool for freshwater fish biodiversity assessment in Europe within the Water Framework Directive (WFD, 2000/60/EC), especially for large rivers and lakes where current fish monitoring techniques have known shortcomings. These new biomonitoring methods based on eDNA show several advantages compared to classical morphological methods. The sampling procedures are easier and cheaper and eDNA metabarcoding is non-invasive and very sensitive, allowing for the detection of traces of DNA. However, eDNA metabarcoding methods need careful standardization to make the results of different surveys comparable. The aim of the EU project Eco-AlpsWater is to test and validate molecular biodiversity monitoring tools for aquatic ecosystems (i.e., eDNA metabarcoding) to improve the traditional WFD monitoring approaches in Alpine waterbodies. To this end, an inter-calibration test was performed using fish mock community samples containing either tissue-extracted DNA, eDNA collected from aquaculture tanks and eDNA samples collected from Lake Bourget (France). Samples were analysed using a DNA metabarcoding approach, relying on the amplification and HTS of a 12S rDNA marker, in two separate laboratories, to evaluate if different laboratory and bioinformatic protocols can provide a reliable and comparable description of the fish communities in both mock and natural samples. Our results highlight good replicability of the molecular laboratory protocols for HTS and good amplification success of selected primers, providing essential information concerning the taxonomic resolution of the 12S mitochondrial marker in describing the Alpine fish communities. Interestingly, different concentrations of species DNA in the mock samples were well represented by the relative DNA reads abundance. These tests confirm the reproducibility of eDNA metabarcoding analyses for the biomonitoring of freshwater fish inhabiting Alpine and peri-Alpine lakes and rivers.
  PubDate: 2022-07-18
  DOI: 10.4081/aiol.2022.10017
  Issue No: Vol. 13, No. 1 (2022)
Spatio-temporal regularities of the White Sea ice regime formation
- Authors: Vyacheslav Nikolaevich Baklagin
  Abstract: The paper presents the spatial and temporal regularities of the course of ice processes in the White Sea, derived from satellite data observations for the period 2004-2020. The dependences, defining indicative dates of the White Sea ice regime of air temperature regime over its water area are given, which can be used for diagnostic and prognostic purposes. It is noted that the connection with the Barents Sea has a significant impact on the course of the ice regime of the White Sea, in particular, on the beginning freeze-up phase and the beginning break-up phase. Comparative analysis of seasonal variations in ice coverage in the White Sea indicates that the course of the ice regime in recent years generally corresponds to how it happened in the second half of the XX century, however, a decrease in average values of ice coverage by 3-7% was recorded throughout the entire period of ice phenomena, which is in line with the global warming trend. Also, the results of the effects of abnormally warm winter on the formation of the White Sea ice regime of 2019-2020 are presented.
  PubDate: 2022-05-30
  DOI: 10.4081/aiol.2022.9849
  Issue No: Vol. 13, No. 1 (2022)

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