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Acta Biologica Marisiensis
Number of Followers: 3

This is an Open Access Journal

Open Access journal
ISSN (Print) 2601-6141 - ISSN (Online) 2668-5124
Published by U of Medicine and Pharmacy of Târgu-Mureş

[1 journal]

HORMONE IMBALANCE IN POLYCYSTIC OVARIAN SYNDROME
- Authors: abmjro_usr
  Abstract: Polycystic Ovarian Syndrome (PCOS) is a disease that is characterized by an increased Gonadotropin-Releasing-Hormone (GnRH) pulsatile frequency, causing an altered LH/FSH ratio. More precisely an increased Luteinizing Hormone (LH) secretion compared to a decreased Follicle-Stimulating Hormone (FSH) secretion leads to the development of hyperandrogenism and to a low-level concentration of estrogens and therefore decreased negative estrogenic feedback in the control axis. The purpose of this review is to connect the physiological Hypothalamic-Pituitary-Ovarian (HPO) axis with said pathology and the ensuring discussion about the possible mechanisms of pathogenesis and guidelines for relieving associated symptoms.
  PubDate: Mon, 27 Mar 2023 06:35:09 +000
CHEMICAL COMPOSITION OF VOLATILE ORGANIC COMPOUNDS OF AN EXTREMELY RARE
AND ENDEMIC ALGERIAN APIACEAE SPECIES, BUNIUM CRASSIFOLIUM BATT.
- Authors: abmjro_usr
  Abstract: Bunium crassifolium Batt. (B. crassifolium) (Apiaceae) is an extremely rare endemic species from the North East of Algeria. In this study, we extracted the volatile organic compounds (VOC) of B. crassifolium Batt. aerial parts using an Agilent G1888 network headspace sampler coupled with an Agilent 7890 GC system. The results revealed the presence of twenty-two (22) compounds, twenty (20) of which were identified as representing 97.48% of the total composition, the major components are: 44.67% of β-Cubebene, 8.82% of β-Caryophyllene, 7.04% of γ-Elemene, 4.70% of δ-Cadinene, 4.11% of γ-Cadinene, 3.77% of Ascaridole and 3.33% of β-Elemene, along with other constituents at a relatively low amount.
  PubDate: Mon, 27 Mar 2023 06:25:21 +000
MEDICINAL PLANTS FROM THE FLORA OF ROMANIA BENEFICIAL IN OSTEOARTHRITIS
AND RHEUMATIC ARTHRITIS
- Authors: abmjro_usr
  Abstract: In this study, we focus on two arthritic diseases that affect a large part of the population, which cause inflammatory disorders of the joints and oxidative stress, which can cause certain degrees of disability. Arthritis is a chronic disease frequently encountered in the world’s population. Osteoarthritis, and rheumatoid arthritis, autoimmune and inflammatory conditions, are two extensive forms of arthritis associated with pain, swelling, and stiffness in the joints and a low standard of life. Many drugs are used in their treatment, associated with some severe side effects and expensive prices. Today there are many studies carried out with extracts from medicinal plants, used in alternative therapy, and effective in these diseases. They are available for clinical use due to the active phytoconstituents that modulate inflammation and are antioxidants. In carrying out this study, electronic databases were screened: Science Direct, PubMed, and Google Scholar, trying to describe these medicinal plants, and elucidate their biological mechanisms of action. The most numerous references were found on the following plants: Arctium lappa, Glycyrrhiza glabra, Nigella sativa, Urtica dioica, etc. vary parts of plants are used such as different types of seeds, roots, leaves, fruit, bark, flowers, or even the whole plant.
  PubDate: Wed, 21 Dec 2022 11:26:25 +000
ISOLATION AND CHARACTERIZATION OF BACILLUS SPP. FOR PLANT GROWTH PROMOTING
PROPERTIES
- Authors: abmjro_usr
  Abstract: A group of free-living bacteria known as plant growth-promoting rhizobacteria (PGPR) inhabit the rhizosphere and aid root development. These rhizobacteria bacteria are vital to the growth of plants and can serve as bio-fertilizer and can enhance food security through green agricultural practices. They exhibit special features which make them potential candidates as bio-fertilizer. Isolation and characterization of rhizobacteria is the first step toward their utilization as bio-fertilizers. Ten rhizobacteria from two different rice farms were isolated and characterized for plant growth promoting properties. The isolated rhizobacteria were identified morphologically, microscopically, biochemically, and molecularly. Plant’s growth promoting properties of these rhizobacteria was also analyzed which includes; Indole 3-acetic acid production (IAA), phosphate solubilisation, hydrogen cyanide production (HCN), ammonia production (NH3), and zinc solubilisation. Out of the ten isolates, three were found to have the best plant growth enhancing properties and were therefore the best candidates as bio-fertilizers. 16SrRNA study and phylogenetic analysis was performed in order to unravel the specie of these three isolates and they were identified as Bacillus subtilis, Bacillus niacini, and Bacillus cereus with accession numbers OM184294, OM1842295 and OM184296 respectively. These isolates have the potential to be used as bio-fertilizer, which would significantly contribute to food security.
  PubDate: Fri, 16 Dec 2022 08:25:38 +000
BIODEGRADATION OF ANTHRACENE AND PHENANTHRENE BY PSEUDOMONAS STUTZERI
(BUK_BTEG1) ISOLATED FROM PETROCHEMICAL CONTAMINATED SOIL
- Authors: abmjro_usr
  Abstract: The United States Environmental Protection Agency (USEPA) has identified 16 substances as priority polycyclic aromatic hydrocarbons (PAHs) that are harmful to humans, including anthracene and phenanthrene. These substances are pervasive pollutants introduced into the environment through anthropogenic and natural processes, causing ecological concerns and necessitating the quest for new strains capable of biodegrading these toxins. A novel strain of the genus Pseudomonas was isolated and molecularly identified based on partial 16S rRNA and phylogenetic analysis as Pseudomonas stutzeri strain BUK_BTEG1 from petrochemical contaminated soil. One factor at a time (OFAT) in Bushnell-Haas (BH) media was used to optimize the strain’s biodegradation conditions. The isolate could grow up to 600 mgL-1 and 400 mgL-1 of anthracene and phenanthrene as the sole carbon source at an optimum pH of 7.0 and 7.5 respectively, inoculum concentration of 4% (v/v), and temperature of 35°C during 72 hours of incubation. The strain could degrade phenanthrene and anthracene to a maximum of 99 and 72 percent, respectively, under ideal conditions. The breakdown products’ GC-MS analysis revealed the existence of the pathway’s main metabolites, catechol, salicylic acid, and derivatives of phthalic acid. The strain exhibits promising potential for use in the bio-cleansing of environments contaminated by PAHs.metabolites.
  PubDate: Thu, 24 Nov 2022 08:36:07 +000
STIMULATION OF PHYSIOLOGICAL PROCESSES IN ST. JOHN’S WORT (HYPERICUM
PERFORATUM L.) SEEDLINGS BY TREATMENTS WITH TRIACONTANOL AND BENZYLADENINE
- Authors: abmjro_usr
  Abstract: Treatment of St. John’s wort plantlets with 1 μM triacontanol and 2 μM benzyladenine stimulates growth and metabolic processes, being an environmental-friendly approach for optimizing the cultivation of these valuable medicinal plants under controlled conditions. When the two growth regulators (a bioactive cuticular wax constituent and a cytokinin) are applied simultaneously, they act synergistically, enhancing each other’s effect on the biomass accumulation and on certain parameters of the photosynthetic light use efficiency, such as the effective quantum yield of photosystem II and the overall vitality index of the photosynthetic apparatus which performs the conversion of light energy into usable forms for carbon dioxide assimilation. The results concerning the interactions between the two externally applied growth regulators during the early development of St. John’s wort plants may lead to a more efficient cultivation of this herbal medicinal product, including the possibility to modulate the production of pharmacologically active metabolites.
  PubDate: Tue, 25 Oct 2022 10:06:16 +000
MORPHOLOGICAL AND ANATOMICAL PROFILE OF CASSIA OCCIDENTALIS (L.) SPECIES
GROWN IN THE REPUBLIC OF MOLDOVA
- Authors: abmjro_usr
  Abstract: The current research deals with the morpho-anatomical characteristics of Cassia occidentalis (L.) (Coffee senna plant), grown in the steppe climate conditions of the Republic of Moldova. This study was performed according to investigated organ in vegetative periods of 2019-2021 years. Morphological and anatomical indices of organs with diagnostic role in identifying species and those with adaptive potential to the action of unfavorable growth factors, have been elucidated. These indices were evaluated in the light of data already reported by other researchers. This morpho-anatomical research has revealed new aspects to support some anatomical features already outlined, but less confirmed in the studies of the last decades and will serve as landmarks for a real clarity of the integral microstructural picture of the C. occidentalis species.
  PubDate: Mon, 10 Oct 2022 12:20:09 +000
A QUICK, SIMPLE, SENSITIVE AND SELECTIVE LC-MS/MS METHOD USED FOR THE
SCREENING OF ETHEPHON, GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID FROM
WATER AND FOOD SAMPLES
- Authors: abmjro_usr
  Abstract: Pesticide use has increased steadily over the years in both industrial farming and local agriculture. One of the most widely used pesticides at a global level, glyphosate, has been controversial for many years and many studies have focused on the effects of this compound on human health. Ethephon is also a controversial ripening agent used to ripen crops more quickly. A basic but crucial step in the study of the effects of their use on human health is the development of adequate analytical methodologies for the quantification of the compounds in relevant samples. For this study a quick, simple and selective method which uses LC-MS/MS was developed for the determination of ethephon, glyphosate and AMPA. The method uses selective fragment monitoring for each analyte and the internal standard, without prior analytical separation. Mobile phase used consisted of aqueous ammonium formate and methanol in isocratic elution and the sample cleanup was made using solid phase extraction (SPE). The method was validated with regards to selectivity, sensitivity, accuracy and precision in accordance with applicable guidelines. After validation the LC-MS/MS method was successfully used to determine ethephon, glyphosate and AMPA residues in ground and surface water, as well as vegetable samples.
  PubDate: Tue, 21 Jun 2022 06:11:19 +000
CHEMICAL CONSTITUENTS OF THE ESSENTIAL OIL FROM SALVIA VERBENACA SSP.
CLANDESTINA FROM ALGERIAN PRE-SAHARA
- Authors: abmjro_usr
  Abstract: The essential oil obtained by hydrodistillation from the aerial parts of Salvia verbenaca (L.) Briq. ssp. clandestina (L.) Pugsl. (Lamiaceae) growing wild in Bou Saâda, pre-Saharan region of Algeria, was analyzed by GC-MS. Sixty-four compounds were detected, representing 95.6% of the whole oil, among them forty five compounds are identified in this sample for the first time. The essential oil of S. verbenaca ssp. clandestena showed the predominance of sesquiterpenes (56.4%) followed by monoterpene derivatives (35.5%). The main constituents were β-pinene (10.2%), spathulenol (8.7%), caryophylene oxide (6.1%), α-pinene (5.2%), germacrene D (5%) and α-gurjunene (4.9%). Chemical composition of the essential oil from our sample may be categorized as sesquiterpene and monoterpene chemotype among the four chemotypes identified for Salvia species.
  PubDate: Thu, 16 Jun 2022 06:23:44 +000
THE ROLE OF NOREPINEPHRINE IN THE REGULATION OF GROWTH, ADHESION AND
INVASION OF PSEUDOMONAS AERUGINOSA IN HUMAN LUNG CARCINOMA CELL CULTURE
- Authors: abmjro_usr
  Abstract: Mammalian hormones are shown to affect not only regulation of mammalian homeostasis but also play roles in cross-talk between microorganisms and their hosts. In our study, the roles of norepinephrine (NE) at two different concentrations on growth alterations, adhesion – invasion of Pseudomonas aeruginosa were investigated. The effects of NE on the growth, adhesion – invasion of P. aeruginosa ATTC 27853 were examined in human lung carcinoma (A549) cell culture. We analysed two concentrations (HNE: 0.04μg/mL and LNE: 0.0017 μg/mL) of NE considering NE’s psychological levels in a healthy individual to imitate in vivo conditions of the host. Bacterial counts of growth and adhesion-invasion were examined by the colony counting method. Growth of bacterium was significantly reduced in the presence of NE at 90’ incubation (p: 0.0004 for high concentration and p: 0.0003 for low concentration); on the other hand, at 180’ minutes incubation, only low concentration reduced the growth (p˂0.0001). While adhesion was increased in the presence of low NE concentration (p: 0.013), there was no alteration in invasion of P. aeruginosa in the presence of NE (p˃0.05). Our results suggested that NE has different effects on the growth and adhesion of P. aeruginosa and these effects depend on […]
  PubDate: Thu, 16 Jun 2022 06:19:41 +000