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Asian Journal of Biology
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This is an Open Access Journal

Open Access journal
ISSN (Online) 2456-7124
Published by SCIENCEDOMAIN international

[66 journals]

Isolation of Bacteriophage against Pseudomonas aureginosa from Male
Urinary Tract Infection (UTI) Specimen
- Authors: John Davison , Bassey Maria
  Pages: 1 - 8
  Abstract: Bacteriophages were isolated from raw sewage and selected based on the size and clarity of plaque that were formed. The selected phages were further enriched and purified. The isolated bacteriophages were found to be able to lyse Pseudomonas aeruginosa isolate from raw sewage. The virulent bacterial viruses of Pseudomonas aeruginosa were found to be of widespread occurrence in nature Bacterial viruses were applied to control pathogenic bacteria in different fields and successfully. Therefore, this work aimed to study is to isolate bacteriophages capable of lysing Pseudomonas aeruginosa as a potential therapeutic alternative to antibiotics.
  PubDate: 2023-05-12
  DOI: 10.9734/ajob/2023/v18i3342
  Issue No: Vol. 18, No. 3 (2023)
Phylogenetic Relationships and Diversity in Leymus (Poaceae, Triticeae)
Based on Simple Sequence Repeats Markers
- Authors: Xin-yi Zhang , Ying-xia Lei, Rui-wu Yang
  Pages: 9 - 18
  Abstract: Aims: The objective of the study was to investigate: (1) the Ns genome donor and elucidate the origins of the Xm genome of Leymus; (2) evaluate the phylogenetic relationships among these species. Methodology: The CTAB (cetyltrimethylammonium bromide) procedure was used to extract total genomic DNA from fresh leaf tissue. A total of 150 pairs of SSR primers were tested to screen those produced polymorphic DNA bands to continue further analysis with Elymus species and 13 diploid perennial species as templates. The GS was used to construct a dendrogram via the unweighted pair group method with arithmetic average (UPGMA) and the SHAN (sequential, hierarchical, agglomerative, and nested clustering) routine in the NTSYS-pc program. Results: The primers WMC475, WMC11 and QWM213 showed more expansion efficiency in the research. There were significant diversity and polymorphism between Leymus and related diploid Triticeae species based on SSR makers. The largest GS coefficient values between Pse. stipifolia and Elymus hystrix indicates that the genetic distance is the closest and has a closer genetic relationship. In clade I, the Leymus species formed a very wellsupported into a small distinct group (Ia) first. Specifically, L. racemosus, L. salinus, L. secalinus and L. triticoides were clustered closely. Conclusion: Psathyrostachys juncea, Psa. huanshanica and Psa. fragilis (Ns genome) clustered together into clade I with Leymus species, which further illustrates Leymus that contains Ns genome is more closely related to Psathyrostachys. St genome and J genome did not participate in the origin of Leymus, and the genetic relationship and genetic distance of Leymus species are related to geographical distribution and environment.
  PubDate: 2023-05-16
  DOI: 10.9734/ajob/2023/v18i3343
  Issue No: Vol. 18, No. 3 (2023)
Evaluation of Yard-long Bean Genotypes at Varied Sowing Dates in the
Sylhet Region, Bangladesh
- Authors: Md. Shahidul Islam, Biswojit Debnath, Argho Chanda , Dwipok Deb Nath
  Pages: 19 - 24
  Abstract: This experiment was conducted in the experimental field of Department of Horticulture, Sylhet Agricultural University (SAU), Bangladesh during 2022. The objective was to evaluate three yard long bean genotypes (BARI yard long bean-1, BARI yard long bean-2 and Kagornatki) in three planting dates (20 March, 20 April and 20 May) under acidic soil conditions of Sylhet. The two factors field experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Yard long bean planted on 20 March recorded the minimum number of days to flower (40.33), the minimum number of days to first harvest (53.33), the maximum number of pods per plant (31.01), the highest individual pod weight (12.25 g), pod length (45.98 cm), per plant pod yield (389.47 g) as well as per decimal pod yield (97.34 kg). The genotype BARI yard long bean-1 was the earliest in flowering (41.66 days). BARI yard long bean-1 had the highest number of pods (27.81) as well as pod yield (309.85 kg) per plant. Although individual pod weight and pod length of BARI yard long bean-1 and Kagornatki were statistically similar, the per decimal pod yield (76.86 kg) of BARI yard long bean-1 was significantly higher than Kagornatki (56.31 kg). Interaction effect showed that the genotype BARI yard long bean-1 planted on 20 March showed the best performance in terms of pod yield per plant (506.56 g) and per decimal (127.22 kg). Therefore, the genotype BARI yard long bean-1 can be recommended to cultivate at 20 March sowing date under acidic soil condition of Sylhet.
  PubDate: 2023-05-16
  DOI: 10.9734/ajob/2023/v18i3344
  Issue No: Vol. 18, No. 3 (2023)
Impact of Lamda-Cyhalothrin on the Biochemical Parameters of Clarias
gariepinus
- Authors: Ogbonna Obiageli Annastesia , Nwamba Helen Ogochukwu, Anukwu John Uchenna
  Pages: 25 - 36
  Abstract: Background: Widespread applications of pesticides such as Lambda cyhalothrin to boost crops production have frequently led to contamination of the fresh water ecosystem in Nigeria. Materials and Methods: In this study, Clarias gariepinus were exposed to sub-lethal concentrations of Lambda-cyhalothrin pesticide. The 96 h LC50 of lambda cyhalothrin to the fish was estimated at 3.98mgl-1. Mortality of 100% and 10% were observed in fish exposed to 12.00 mg l-1 and 1.25 mg l-1 of Lambda cyhalothrin respectively as compared to no mortality recorded in the control group. Varying degrees of abnormal behaviours like air gulping, hyperactivity, erratic movement, skin discoloration and jerky movements were observed during the 96 hrs exposure period of the fish to Lambda compared to the control. Exposure to sub-lethal concentrations of Lambda cyhalothrin at 0, 0.25, 0.50 and 1.00 mgl-1 and for 15 days at 5 days intervals that is 5, 10 and 15days led to changes in the biochemical parameters. Results: In the Biochemical parameters analysed, there was significant (P<0.5) increase in the mean values of Albumin, Aspartate Amino Transfarase (AST), Alanine Amino Transaferase (ALT), Alkaline Phosphate (ALP) and Creatinine compares to the control group. There was no significant difference (p>0.05) between the values of water quality assays in control and treated group. Conclusion: The biomarkers measured could be useful tools for monitoring effects of other pesticides on aquatic organisms. However, further studies could be done to investigate their mode of action to strike a balance between protection of aquatic biota and discharges of these pesticides and their metabolites to aquatic environments.
  PubDate: 2023-05-25
  DOI: 10.9734/ajob/2023/v18i3345
  Issue No: Vol. 18, No. 3 (2023)