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BioLink : Jurnal Biologi Lingkungan, Industri, Kesehatan
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ISSN (Print) 2356-458X - ISSN (Online) 2597-5269
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EFFECT OF COMBRETUM INDICUM LEAF EXTRACT ON BLOOD SUGAR LEVELS AND
PANCREAS HISTOLOGY OF ALLOXAN INDUCED MICE
- Authors: Anggita Endar Pertiwi, Retno Aryani, Imam Rosadi, Rudy Agung Nugroho
  Pages: 126 - 137
  Abstract: Diabetes is a health problem that affects more than half a billion people in the world. Several plants are known to have anti-diabetic activity, one of which is Dutch jasmine (Combretum indicum). This research was conducted to determine the effect of Combretum indicum leaf extract on blood sugar levels and histology of islets of Langerhans in mice. A total of 36 male mice aged 6-8 weeks were divided into 6 treatment groups, namely normal control (KN) without treatment; negative control (K) with alloxan treatment; positive control (K+) with alloxan and glibenclamide treatment; P1 treated with alloxan and Combretum indicum leaf extract at a dose of 100 mg/kgBW; P2 with treatment with alloxan and Combretum indicum leaf extract at a dose of 200 mg/kgBW; P3 with alloxan treatment and Combretum indicum leaf extract at a dose of 200 mg/kgBW. Mice that have been induced by alloxan will be treated with leaf extract and glibenclamide for 20 days. Blood sugar levels 3 days after alloxan induction were checked and checked again on the 10th and 20th days of treatment by taking blood through the blood vessels in the mice's tails and then measuring them using a glucometer. The pancreas was dissected on the 20th day and pancreatic histology preparations were made for histological analysis. The results of this study indicate that Combretum indicum leaf extract has antihyperglycemic effects by protecting islets of Langerhans cells from alloxan-induced oxidative stress.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11159
  Issue No: Vol. 10, No. 2 (2024)
ANTIBIOFILM ACTIVITY OF MANGOSTEEN (Garcinia mangostana L.) LEAF EXTRACT
AGAINST COLLECTION OF BACTERIAL ISOLATES FROM DIABETIC ULCERS
- Authors: Derina Dwifrila Ridhani Gubali, Nur Rasdianah, Juliyanty Akuba, Widy Susanti Abdulkadir, Wiwit Zuriati Uno
  Pages: 138 - 148
  Abstract: Mangosteen (Garcinia mangostana L.) is a plant that contains many benefits and has the potential to be a medicinal plant in treating various diseases. Mangosteen leaves contain flavonoids, saponins, tannins, alkaloids and terpenoids which are known to have antibacterial and antibiofilm properties. This anti-biofilm agent is an alternative treatment for diabetic wound infections where cases of antibiotic resistance have increased. One of the causes of resistance is the biofilm formed by infectious bacteria. This research is a type of laboratory experimental research which aims to determine the anti-biofilm activity of mangosteen leaf extract against biofilms formed by a collection of bacterial isolates from diabetic wounds. The antibiofilm activity test carried out consisted of a cell attachment prevention test, a biofilm formation inhibition test, and a biofilm destruction test using the crystal violet staining method using the tube method and measuring optical density values on a UV-Vis spectrophotometer. The concentrations of mangosteen leaf extract used are 60%, 80% and 100% as well as control – and control +. The results of the antibiofilm test showed that mangosteen leaf extract had the best activity in inhibiting biofilm formation, preventing cell attachment, and destroying biofilm, respectively. The three most optimal activities were found at a concentration of 100%, with an inhibition percentage of 50.51%, prevention of 32.56%, and destruction of 5.63%.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11185
  Issue No: Vol. 10, No. 2 (2024)
ACTIVITY TEST OF BESTSELLING MOISTURIZERS ON SHOPEE IN TREATING SKIN
PROBLEMS
- Authors: Eva Sartika Dasopang, Desy Natalia Siahaan, Muharni Saputri, Salsa Irnabila
  Pages: 149 - 156
  Abstract: Shopee is an e-commerce widely used by Indonesians to buy and sell products and can shop safely. Shopee shows that in 2020, moisturizers was the top selling product in the beauty category. The moisturizers sold have different ingredients, come from many brands, and have different price points. The objective of the study is to compare the 3 best-selling moisturizers in the shopee category that are more effective in improving skin problems. This study was a quantitative study using a true experimental design with a posttest only control group design consisting of 4 groups, namely 3 groups using 3 best-selling moisturizer brands based on the shopee category and 1 control group. The sample of this study was selected based on the inclusion criteria consisting of 20 volunteers and the test was conducted for 4 weeks of application on the volunteers' skin by looking at the measured parameters of moisture, pores, blemishes and wrinkles. The results showed that the activity test of moisturizer A had a faster recovery in increasing moisture content (54.8%), shrinking pore size (23.8%), reducing blemishes (19.9%) and wrinkles 28.9%.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.10704
  Issue No: Vol. 10, No. 2 (2024)
CONSUMER KNOWLEDGE, ATTITUDE, ENVIRONMENT AND INCOME TOWARD COOKING OIL
WASTE
- Authors: Veza Azteria, Muhammad Sayyid M, Putri Handayani, Devi Angeliana K
  Pages: 157 - 167
  Abstract: Used cooking oil is waste oil that comes from a type of cooking oil that has been used repeatedly. If not managed properly, it can become a source of environmental pollution. Managing used cooking oil still needs to be in a good category. Preliminary studies from this research found that as many as 60% of people in the Cengkareng Village area, West Jakarta, need to manage used cooking oil properly. This research aims to determine factors related to knowledge, attitudes, income and environment in managing used cooking oil. The sample used was 141 homemakers using purposive sampling. The data collection methods used were interviews and observation. This research uses independent variables (Knowledge, Attitudes, Income Level, and Environment) and dependent variables (Used cooking oil management behaviour). The analysis used is descriptive analysis with the chi-square test. Based on the research results, it was found that the highest proportion of used cooking oil management behaviour was poor (51.1%), good knowledge (54.6%), negative attitude (60.3%), sufficient income level (81.6%), and bad environment (93.6%). %). There is a relationship between knowledge (p-value = 0.001) and community behaviour in managing used cooking oil and a relationship between attitude (p-value = 0.001) and behaviour in the community driving used cooking oil. There is a need for interaction and cooperation between societal stakeholders to improve public awareness of cooking oil management to create a zero-waste environment.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11147
  Issue No: Vol. 10, No. 2 (2024)
ANALYSIS OF MINERAL CONTENT IN ZAM-ZAM WATER CIRCULATION IN
PADANGSIDIMPUAN CITY
- Authors: Fatma Suryani Harahap, Heni Mulyani Pohan, Rabiyatul Adawiyah Siregar, Elisa Elisa, Jalilah Azizah Lubis, Nur Latifah
  Pages: 168 - 176
  Abstract: This research was motivated by the existence of Zam-zam water trade in Padangsidimpuan City, while the Saudi Arabian government prohibited the export of Zam-zam water. This causes people to suspect that the quality of Zam-zam water circulating in the market is not the same as the Zam-zam water found in Mecca. Therefore, research needs to be carried out to answer these concerns. This research aims to analyze the mineral content of labeled packaged Zam-zam water circulating in Padangsidimpuan City compared with Zam-zam water brought from Mecca City. The mineral concentrations tested were sodium and chloride. This research uses laboratory experimental methods, including quantitative analysis. According to the type of research, the data analysis technique used is to compare the concentration of each mineral in the water labeled Zam-zam with the mineral concentration in the Zam-zam water used as a control. The research results showed that Zam-zam water obtained from Mecca City contained 42.4 mg/L chloride, 2.06 mg/L sodium, while the labeled Zam-zam water circulating in Padangsidimpuan, namely sample 1, contained 160.7 mg/L chloride. sodium 2.06 mg/L in sample 2 contains 50.5 mg/L chloride, sodium 2.17 mg/L. The three Zam-Zam samples circulating in Padangsidimpuan that have been tested show that the quality of Zam-Zam water is suitable for consumption based on determining a good water quality test, namely at the suitability threshold.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.10933
  Issue No: Vol. 10, No. 2 (2024)
DETECTION OF DANGEROUS FOOD ADDITIVE IN TAKJIL SNACKS DURING OF RAMADHAN
- Authors: Ahmad Irfandi, Meithyra Melviana Simatupang, Erna Veronika, Veza Azteria
  Pages: 177 - 184
  Abstract: Food is a basic human need, necessary to meet daily needs. Human health is greatly influenced by what they consume. Food products added dangerous ingredients can cause serious health effects for humans. The aim of this research was to detect substances of formaldehyde and borax in snacks in the month of Ramadhan (takjil) at Bekasi City. This was descriptive observational research design by direct examination for formalin and borax from the food samples. The population in this study were all traders who sold Takjil on the roadsides in Bekasi City. Sample selected used an incidental sampling technique by taking 8 food samples which. Based on references, they were proven to contain dangerous chemicals. Based on the research results, 2 food samples were found positive containing dangerous food additives, namely 1 sample of lontong (12.5%) was positive for containing borax and 1 sample of martabak tofu (12.5%) was positive for containing Formalin. Seasonal traders who Sell Takjil food during the month of Ramadan may not necessarily be safe. Therefore, it is recommended that health workers from Puskesmas must frequently inspect the food products and socialize the importance of using safe food additives.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11219
  Issue No: Vol. 10, No. 2 (2024)
EFFECT OF FERMENTATION OPTIMIZATION WITH PH CONTROL ON ACTINOMYCETES
ISOLATE AS ANTIBACTERIAL STAPHYLOCOCCUS AUREUS
- Authors: Anastasia Joanne, Meiskha Bahar, Hikmah Muktamiroh, Yuni Setyaningsih
  Pages: 185 - 195
  Abstract: Actinomycetes are Gram-positive bacilli noted for their secondary metabolites that are effective as antibacterials. Production of the antibacterial compounds is determined by nutrition, temperature, pH, and fermentation time. This study aims to study the antibacterial activity of Actinomycetes isolates from Bogor Botanical Gardens soil by fermentation optimization with pH control on the 3rd, 4th, and 5th day against the growth of Staphylococcus aureus bacteria. Actinomycetes isolates were inoculated on Starch Casein Agar (SCA) and fermented in liquid media containing 7 g glucose, 14 g mannitol, 14 g peptone for 5 days with the pH controlled within the range of 6.2–6.8. Antibacterial test method against S.aureus using well diffusion method with Nutrient Agar (NA) media. The inhibition zone diameter was measured every 24 hours and and the results obtained were 3.73 mm; 6.05mm; and 5.34mm. Those data were analyzed using the Kruskal-Wallis test and then continued with the Mann Whitney test which had significant differences between groups. The conclusion of the study is Actinomycetes isolates have antibacterial potential against the growth of S. aureus with the largest average diameter of inhibition zone in the 4th day fermentation group, which had moderate inhibition.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.10529
  Issue No: Vol. 10, No. 2 (2024)
THE POTENTIAL UTILIZATION OF RASAU FIBER (Pandanus helicopus) AS AN
ALTERNATIVE NON-FOOD INDUSTRY MATERIAL TOWARDS A CREATIVE ECONOMY
- Authors: Latifah Nur A’in, Jumrodah, Atin Supriatin
  Pages: 196 - 209
  Abstract: Pandanus helicopus is one of the fibrous plants that is considered a nuisance so that its utilization is still lacking. Meanwhile, P. helicopus's fiber has the potential to be used in industry, especially in the manufacture of food brushes. The objective of this study is to identify the potential of P. helicopus's fiber, which is used as an alternative to non-food industrial materials, especially brushes that are halal toward the principle of creative economy. This study is experimental research by combining two methods, there are exploration and experiment laboratory. The results showed that P. helicopus leaf fibers have the characteristics of fibers that are quite fine, strong and elastic. Based on the laboratory tests, it was found that fibers became stronger and finer when given immersion treatment in NaOH solution with the most effective concentration of 7.5% with the test results of a fineness level of 2462.88 Tex, a tenacity value of 145.07 mN/Tex and 14.80 gf/Tex, and fiber elasticity of 19.56%. The characteristics of P. helicopus leaf fiber has the potential to be used as an alternative material in the industrial field, especially in the manufacture of halal plant fiber brushes. The utilization of P. helicopus's fiber in industry can be a creative innovation that can realize the principles of creative economy.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11195
  Issue No: Vol. 10, No. 2 (2024)
THE INFLUENCE OF WATERFALL DISTANCE ON BRYOPHYTES DIVERSITY IN THE COBAN
RONDO WATERFALL AREA, PUJON, MALANG
- Authors: Salsabila Rofifah, Rahmatin Ilmiatunnisa, Wardatul Hakimah, Anggi Kusumaning Tias, Agus Rizki Dwi Rahmawan, Dhiva Ayu Agustin, Indra Fardhani
  Pages: 210 - 221
  Abstract: Bryophytes is one of the various plant groups that have an important role in biodiversity. Bryophytes have an essential role in environmental ecology, including mosses contributing to the nutrient and water cycle and the carbon exchange cycle. This research aims to determine the effect of waterfall distance on bryophyte diversity in the waterfall area. This research uses an exploratory descriptive method with data collection locations at Coban Rondo Waterfall, Pujon District, Malang Regency. The research was carried out by collecting data directly in the field by observing and exploring according to the conditions at the research location. The objects in this research are classes bryophytes that grow around the waterfall area by creating three plots measuring 5m x 5m with the installation at the wet zone which is located in the waterfall area <25 m, the humid zone which is 25-50 meters from the waterfall, and the dry zone which is more than >50 meters from the waterfall. Each bryophyte found in a single plot in three zones will be analyzed by considering the factors of temperature, humidity, light intensity, and soil pH. The results of the study showed that there was an influence of waterfall distance on bryophyte diversity. The closer the location where bryophytes grow to a waterfall, the more diverse the number of species.
  PubDate: 2024-02-12
  DOI: 10.31289/biolink.v10i2.11014
  Issue No: Vol. 10, No. 2 (2024)
BIODIVERSITY OF ANGIOSPERMS IN SUMBERAWAN TEMPLE, SINGOSARI, MALANG
DISTRICT
- Authors: Savina Tunazjah, Ifta Alifia Zahrani, Khoirun Nisa, Siti Zulaika Maulida Dewi, Akmal Rizky Prasetya, Irbah Jihan Nabila, Cornellia Wening Manggalgita, Indra Fardhani
  Pages: 222 - 235
  Abstract: Sumberawan Temple is a tourist area that is inextricably linked to diversity of plants. One of them is angiosperm plants. Angiosperms occupy about 90% of all plant species and have 250,000 species. The objective of this study is to determine the biodiversity of angiosperm plants in Sumberawan Temple area which is located in Singosari, Malang Regency. The research method used was vegetation analysis using a single plot measuring 20 x 20 m2. The shape of the plot used wass square with an area of 400 m2. There were 12 types of angiosperm plant species in 9 different families. To determine the species richness index using the Margalef index, the diversity index using the Shannon Weinner index and the evenness index using the Evenees index. The findings indicated that species richness was in the low category, diversity and evenness were in the high category. It is due to the fewer species in the area but the greater variety of species in Sumberawan Temple area. The diversity of species indicates the number of species that coexist.
  PubDate: 2024-02-13
  DOI: 10.31289/biolink.v10i2.11025
  Issue No: Vol. 10, No. 2 (2024)
INVENTORY OF INVASIVE INSECT SPECIES IN COBAN TALUN, BATU CITY
- Authors: Priska Anggun Saputri, Jihan Fahira Adinda Putri, Amirah Rusdanti Zahwa, Dyah Prasetyaningsih, Nurul Afifah, Sherlita Crisne Denatalia, Indra Fardhani
  Pages: 236 - 246
  Abstract: Insects are the greatest number of animal species on earth. Invasive species are insects that are non-native to a particular area, and negatively affect other organisms in their ecosystem. This study examines the diversity of invasive insect species with the aim of determining the diversity of invasive insect species found in Coban Talun, Batu City, effective and sustainable invasive insect control strategies, as well as conservation actions that need to be taken to maintain the balance of the ecosystem in Coban Talun. This study used a descriptive method using exploration techniques at three observation locations to collect data. This method used was observation of the focus of the problem under study, namely invasive insects which are the object of research. The data collection technique was carried out by exploring the location under study by observing, documenting, describing and identifying specimens using various literature. The results of the study obtained were the discovery of several types of invasive insects including black cockroaches (Phyllodromica maculata), large heath butterflies (Coenonympha tullia), green grasshoppers (Tettigonia viridissima), flies (Musca domestica), Mexican bean beetles (Epilachna varivestis), and soldier beetles (Rhagonycha recta). Invasive types of insects need population control by using natural insecticides so that the ecosystem in Coban Talun remains balanced.
  PubDate: 2024-02-13
  DOI: 10.31289/biolink.v10i2.11010
  Issue No: Vol. 10, No. 2 (2024)

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