JournalTOCs

BioLink : Jurnal Biologi Lingkungan, Industri, Kesehatan
Number of Followers: 0

This is an Open Access Journal

Open Access journal
ISSN (Print) 2356-458X - ISSN (Online) 2597-5269
Published by Universitas Medan Area

[8 journals]

INTERACTION OF CO2 AND LIGHT AVAILABILITY ON PHOTOPHYSIOLOGY OF TROPICAL
COCCOLITOPHORIDS (EMILIANIA HUXLEYI, GEPHYROCAPSA OCEANICA, AND
OCHOSPHAERA SP.)
- Authors: Nita Rukminasari, Muhammad Lukman, Khusnul Yaqin
  Pages: 133 - 150
  Abstract: The study to examine the calcification rate, adaptation, and the biotic response of three tropical coccolithophorids (Emiliania huxleyi, Gephyrocapsa oceanica, and Ochosphaera sp) to changes in CO2 concentration. Three selected calcifying coccolitophorids were grown at batch culture with CO2 system at two levels of CO2 (385 and 1000 ppm) and two light dark periods. The parameters measured and calculation including growth rate, particulate organic carbon content, particulate inorganic carbon content, chlorophyll a, cell size, photosynthetic, organic, inorganic carbon production, photosynthesis, and calcification rate. The results showed that there was a different response to carbonate chemistry changes and dark and light periods in any of the analyzed parameters. The growth rate of three selected calcifying microalgae tested was decreasing significantly at high concentrations of CO2 (1000 ppm) treatment on 14:10 hour light: dark periods. However, there was no significant difference between the two CO2 concentrations where they were illuminated by 24 hours light in growth rate. The increasing CO2 concentration and light-dark periods were species-specific responses to photosynthesis and calcification rate for three selected calcifying microalgae.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7912
  Issue No: Vol. 9, No. 2 (2023)
12 TEXTILE DYES DECOLORIZATION BY COPPER-RESISTANT-BACTERIA KLEBSIELLA
GRIMONTII, SHIGELLA FLEXNERI, ENTEROBACTER CLOACAE ISOLATED FROM CISADANE
RIVER TANGERANG
- Authors: Wahyu Irawati, Vania Austine Callista Timotius, Ruben Patrick Adhiwijaya, Bellen Mouretta, Steven Anggawinata, Eunike Bunga Marvella
  Pages: 151 - 162
  Abstract: Dyes and copper are dangerous contaminants because they are toxic. Bioremediation using indigenous bacteria is the best solution to overcome water pollution. Copper resistant bacteria usually have resistance to dyes thereby helping the bioremediation of dye and copper wastes. This study aims to examine the ability of indigenous bacteria isolated from the Cisadane River, namely Klebsiella grimontii IrCis3, Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 in terms of resistance and ability to decolorize 12 textile dyes namely methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, basic fuchsin, reactive orange, dispersed orange, remasol red, wantex yellow and wantex red. The results showed that Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 were resistant to all dye concentrations of 200 and 500 ppm except Klebsiella grimontii IrCis3 did not grow on malachite green and basic fuchsin at concentrations of 200 ppm and methylene blue, malachite green and basic fuchsin concentration of 500 ppm. Only Shigella flexneri IrCis5 has the ability to decolorize 200 ppm basic fuchsin up to 87.23% after 3 days of incubation. The addition of 3 mM CuSO4 reduced the ability to decolorize Shigella flexneri IrCis5 to 0.57%.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7731
  Issue No: Vol. 9, No. 2 (2023)
ANALYSIS OF NEW PRIMER PAIR CANDIDATES OF rbcL GENE FOR IDENTIFICATION OF
MICROALGAE SCENEDESMACEAE
- Authors: Megga Ratnasari Pikoli
  Pages: 163 - 173
  Abstract: Scenedesmaceae is one of the microalgae groups that has been widely studied as promising biodiesel feedstock. Its morphological identification is often confused by environmental changes, so it requires molecular identification as well. The current study aimed to obtain primer pair candidates that identify the Scenedesmaceae based on the rbcL gene. The research used bioinformatics tools, which harvested rbcL protein sequence data, performed multiple sequence alignments, and designed primers based on conserved and less-conserved regions. The best left and right primers selected based on sequence length, melting temperature, 3' end stability, number of hairpins, and self-dimers, were paired, and three candidates were obtained. The three pairs were examined based on melting temperature difference, number of hetero-dimers, length of amplified nucleotide product, number of hits, and number of genera captured from the GenBank. Sce-16 (F, 5'-TGGTCGTGCTGTTTATGAATGT-3' and 1_RL, 5'-TGCCAAACATGAATACCACCA-3'), which is back-translated according to Hariotina sp. (AOY36008.1), is the most preferred candidate compared to the other two pairs after discussing their advantages and disadvantages. In the future, the proposed primer candidate needs to be validated through in vitro amplification with some optimizations to eliminate potential weaknesses.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7918
  Issue No: Vol. 9, No. 2 (2023)
CARBON STORAGE IN SEAGRASS BEDS IN THE LITORAL ZONE OF SANCANG BEACH,
GARUT
- Authors: Dedi Koswara, Diana Hernawati, Diki Muhamad Chaidir
  Pages: 174 - 182
  Abstract: The vast expanse of seagrass beds in coastal areas can provide many ecosystem services, one of which is the ability of seagrass beds to absorb and store carbon dioxide (CO2). Carbon dioxide gas (CO2) is one of the components of greenhouse gases that contribute to global warming. So the role of seagrass is very important in the ecosystem. This study aims to determine carbon storage in seagrass in the littoral zone of Sancang Beach, Garut Regency, which was carried out in April-June 2022. Determination of potential carbon storage was carried out by taking seagrass samples with a size of 20 cm x 20 cm, then drying to a constant weight. and calculated using a carbon conversion factor for seagrass biomass of 0.34. The results showed that the carbon content at Station 1 Cibako (145.9331 g C/m2) was higher than Station 2 Cikujangjambe (110.026 g C/m2) and Station 3 Ciporeang (117.771 g C/m2). The average carbon content at Sancang beach is 124,577 g C/m2. The conclusion is that in general the carbon storage below the substrate (below ground) in seagrass in the Litoral Zone of Sancang Beach has a greater value than the value of carbon storage above the substrate (above ground).
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7778
  Issue No: Vol. 9, No. 2 (2023)
QUALITATIVE ANALYSIS OF SALAK LEAF EXTRACT (SALACCA SUMATRANA)
- Authors: Jalilah Jalilah Azizah Lubis, Sahlan Tuah Harahap, Fatma Suryani Harahap
  Pages: 183 - 189
  Abstract: Qualitative analysis research of salak leaf extract has been carried out, where the salak leaf extract Padangsidimpuan has characteristic with a larger size and does leaf has a meat which has a distinctive sour taste, because of its acid content, it is processed in the form of salak leaf (Salacca sumatrana) which aims to analyze the levels of secondary metabolites in salak leaf which are expected to be a cholesterol-lowering herbal medicine. With the appropriate qualitative analysis of salak leaf using the extraction method, namely the maceration method with methanol solvent, the results obtained that salak leaf contains Secondary metabolites consisting of alkaloids, phenolics, saponins, tannins, terpenoids, however, it does not contain flavonoids and steroids.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.8219
  Issue No: Vol. 9, No. 2 (2023)
UTILIZATION OF EUCALYPTUS GRANDIS LEAF WASTE IN THE PRODUCTION OF VCO
BASED LIQUID SOAP
- Authors: Desi Ardilla, Khairunnisa Rangkuti, M. Taufik
  Pages: 190 - 200
  Abstract: Eucalyptus grandis still not much used, especially the leaves. Besides being used as an industrial material, Eucalyptus is also used as a medicinal plant to cure infectious diseases, fever and rheumatism as well as being an antiseptic and can be applied in the form of bath soap. The purpose of this study was to determine the best combination of liquid body soap with the addition of eucalyptus leaf extract with various concentrations. The research method used was a laboratory experiment using a completely randomized design (CRD) method with the addition of eucalyptus oil A = 1% (w/w), B = 2% (w/w), C = 3% (w/w), D = 4% (w/w) and E = 5% in total VCO 80 oil, 40% KOH = 35 g, glycerin = 60 g, propyleneglycol = 35 g, three times the repetition. The parameters observed were the physicochemical properties of soap based on SNI Liquid Bath Soap 06-4085-1996 including free alkali content, namely A = 0.072%, B = 0.052%, C = 0.078%, D = 0.090$, E-), 104% , pH value, A= 8.36, B= 8.78, C= 8.80, D= 8.85 and E=8.94, Specific Gravity A= 1.008, B= 1.018, C= 1.009, D= 1.190, E = 1.014.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.8535
  Issue No: Vol. 9, No. 2 (2023)
ANALYSIS OF FORMULATION MEDIA FOR PLANTING OYSTER MUSHROOM (Pleurotus
ostreatus)AGRICULTURAL WASTE BASED MATERIALS
- Authors: Siti Mardiana, Ellen Lumisar Panggabean, Indah Apriliya, Muhammad Usman
  Pages: 201 - 208
  Abstract: The effort of Indonesian people to fulfill their protein intake in an economically accessible way is by producing and consuming oyster mushrooms, because their protein content was viable as an alternative to animal-based products. Combination of sugarcane bagasse, palm fronds (PKS), and sawdust can be used as growing medium for oyster mushrooms. The aim of this study was: (1) to analyze the change in the pH of the combination media formulation of Palm Oil Frond Waste (PKS), sugarcane bagasse, and sawdust; (2) identifying the composition of nutrients in the aforementioned agricultural waste as a growing medium for oyster mushroom. This study was composed of two steps: 1) Preparing the composition of the growing medium from agricultural waste; and 2) Analyzing nutrient composition of the growing medium of oyster mushroom. The research was conducted by using Analysis of Variance (ANOVA) with a Non-Factorial Completely Randomized Design (RAL). The combination of oyster mushroom growing medium from palm fronds, sugarcane bagasse, and sawdust has different pH levels and nutritional contents. The combination of M0 – M8 oyster mushroom growing medium has the suitable level of pH to grow the mushrooms at 6,5 – 8,0 pH. From the formulation analysis of nutrient in the combination of M0 – M8, it can be used as a reference for the nutrient content of oyster mushrooms growing medium from palm fronds and sugarcane bagasse.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7504
  Issue No: Vol. 9, No. 2 (2023)
SOIL QUALITY IN ORGANIC AGRICULTURAL LAND: STUDY OF CHEMICAL ANALYSIS AND
SOIL MICROBIOLOGY
- Authors: Melfa Aisyah Hutasuhut, Husnarika Febriani, Leni Widiarti
  Pages: 209 - 218
  Abstract: Increased public awareness for a healthy diet must be balanced with successful cultivation. Organic farming system is the right choice since it leaves all non-organic components. This study aims to identify the chemical and microbiological properties of agricultural soils that apply organic systems located in Batang Buluh Village, Pematang Johar, Deli Serdang Regency, North Sumatra. Chemical analysis was carried out at Socfindo Laboratory in Medan, including testing the pH of H2O, total P and K, C Organic, N Kjehldahl, and CEC (Cation Exchange Capacity). Soil microbiological tests were carried out at Medan Regional Health Lab including gram staining tests and biochemical tests. The results of chemical analysis from the analysis of pH H2O, P and total K, Organic C, N Kjehldahl, and CEC (Cation Exchange Capacity) at the edges and middle each obtained that was pH 5 -6, soil total P content was 0.0260% up to 0.450%, available K analysis, namely 0.200% and 0.210%, organic C content obtained results of 0.970% and 0.630%, N content using the KJehldahl method was 0.150% and 0.090%, and CEC obtained results of 14.330 me/100 and 10.090 me/100 g. Related species of Bacillus contained in the soil were Gram-positive, rod-shaped, spore-forming, aerobic or facultative anaerobic, motile bacteria with peritrichous flagella.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.8178
  Issue No: Vol. 9, No. 2 (2023)
THE PRESENCE OF PLUMBUM (Pb) AND CADMIUM (Cd) HEAVY METALS OF BROILER
CHICKEN
- Authors: Zahra Layli Rochimi, Rahayu Sri Pujiati, Prehatin Tri Rahayu Ningrum
  Pages: 219 - 230
  Abstract: The level of meat production from purebred chickens in East Java Province from 2009 to 2019 reached 510,535.29 tons. The existence of heavy metal contamination in chickens are dangerous for human health due to the lack of good facilities and infrastructure on broiler farms including land and locations that must be environmentally friendly, and when rearing chickens in paying attention to the needs of water and feed for broiler chickens that are not polluted by heavy metals. The objective of this study was to describe the levels of heavy metals in chicken meat at X Chicken Farm in Kertosari Village, Pakusari District, Jember Regency. This research was a type of descriptive research. The subjects of this study were 20 samples of broiler chicken, well water, and 4 respondents. The sampling technique used purposive sampling. Data analysis used descriptive to describe the use of chicken feed, sources of drinking water, and the presence of heavy metal levels of Pb and Cd in chicken. The result indicate that as many as 20 samples of chicken meat contained levels of heavy metals Pb and Cd below the quality standard values according to Indonesian Nasional Standard (SNI) 7387:2009), namely (pb 0.3/kg) and (Cd <0.0200) while for water drinking water used comes from drilled wells containing Pb 0.001 mg/l and Cd 0.001 mg/l..
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.7571
  Issue No: Vol. 9, No. 2 (2023)
THE EFFECT OF WEED MANAGEMENT USING HERBICIDE ON CORN RESULTS (Zea mays L)
- Authors: fitria, Nana Trisna Mei Br Kabeakan, Nurhajijah
  Pages: 231 - 238
  Abstract: Weeds are undesired plants to grow so weed management is expected to overcome losses caused by weeds. One way to manage weeds using herbicides is the chemicals used for weed management in plants. This study aims to determine the influence of weed management using herbicides on corn yields. The research was conducted in Sekoci Village, Besitang District, Langkat Regency, North Sumatra in April-June 2021. This study used a non-factorial Randomized Block Design (RBD), with 4 treatments and 3 replications, namely contact, systemic, weeding and weeds without management. The results showed that herbicide management significantly affected 100 seed weight, cob weight per plot and seed weight per plot, but had no significant effect on weed without management. It indicates that the treatment of weed management using herbicides affects plant growth and yield. Weed management for farmers is very important to prevent competition for nutrients between weeds and corn.
  PubDate: 2023-02-15
  DOI: 10.31289/biolink.v9i2.8569
  Issue No: Vol. 9, No. 2 (2023)