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Arquivos do Museu Dinâmico Interdisciplinar
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This is an Open Access Journal

Open Access journal
ISSN (Print) 1980-959X
Published by Universidade Estadual de Maringá

[9 journals]

IDENTIFICATION OF GLUCAGON-LIKE PEPTIDE-1 RECEPTOR LIGANDS AND THEIR
IMPACTS IN THE TREATMENT OF TYPE 2 DIABETES
- Authors: Luiz Gustavo Antunes Pessoa, Ananda Malta, Paulo Cézar de Freitas Mathias, Wanderley Dantas dos Santos, Flávio Augusto Vicente Seixas
  Abstract: Diabetes mellitus is a chronic disease responsible for one of the world's most common public
  health problems. Its prevalence, comorbidities, and medical costs assume a dramatic scale and
  an efficient response to these projections is the intensified search for new agents and
  approaches. Therefore, for the development of potential glucagon-like peptide-1 receptor
  (GLP-1R) agonists, a virtual screening technique based on molecular docking using the
  crystallographic structure of human GLP-1R as a target was employed in two product
  libraries natural, Zinc15 and Sigma-Aldrich. The structure of the protein (PDB id 6X1A)
  bound to Pfizer compound PF-06882961 was modeled by homology. The best-presented
  model was inserted into a lipid bilayer and subjected to structure minimization, redocking,
  virtual screening, and molecular dynamics simulations. After simulations, 39 molecules were
  selected and analyzed by predicting bioactivity and pharmacokinetics resulting in 4 ligands
  (Zinc1901002, Zinc1901002m, 336188752 e 336185530). The ligands were inserted into the
  6X1A crystallographic structure to analyze the conformational changes and the stability of
  protein-ligand interactions through molecular dynamics simulations for 50 ns. We observed
  that the ligands coupled to GLP-1R remained stable without conformational changes
  throughout the simulation, indicating that the selected ligands can act as potential GLP-1R
  agonists.
  PubDate: 2023-05-11
  Issue No: Vol. 27, No. ESPECIAL (2023)
LIVER METABOLIC EFFECTS OF A HIGH-FAT LOW- CARBOHYDRATE DIET IN RATS: A
STUDY UNDER DIFFERENT ENERGY CONDITIONS
- Authors: Carla Indianara Bonetti, Mateus José Oliveira, Evelyn Silva Moreira, Bruna Lopes Correia, Gabriel Conti Souto, Lívia Bracht
  Abstract: The beneficial effects of high-fat, low-carbohydrate (HFLC) diets on glucose metabolism
  have been questioned and its effects on liver metabolism are not totally clear. This work
  aimed to evaluate the HFLC diet effects under different energy conditions on glucose
  homeostasis, fatty liver development and hepatic gluconeogenesis using the isolated perfused
  rat liver. HFLC diet (79% fat, 19% protein, and 2% carbohydrates in Kcal%) was
  administered to rats over four weeks under three conditions: hypercaloric; isocaloric and
  hypocaloric (energy reduction of 20%). Fasting blood glucose levels and total fat in the liver
  were higher in all HFLC diet rats. Oral glucose tolerance was impaired in isocaloric and
  hypercaloric groups, although insulin sensitivity wasn´t altered. HFLC diet also caused
  marked liver metabolic alterations: higher gluconeogenesis rate from lactate and a reduced
  capacity to catabolize alanine, the latter effect more intense in the hypocaloric condition. We
  concluded that, even when HFLC diets are used for weight loss, our data imply that they can
  potentially cause harmful consequences for the liver.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68207
  Issue No: Vol. 27, No. ESPECIAL (2023)
EARLY PPARα ACTIVATION PROTECT AGAINST HEPATIC MICROSTEATOSIS INDUCED
BY POST-NATAL OVERFEEDING
- Authors: Ana L. M. Assakawa, Lucas P. J. Saavedra, Scarlett Raposo, Naiara Lucredi, Maria N. C. Peres, Silvano Piovan, Gessica Gonçalves, Letícia Barbosa, Jurandir Comar, Douglas L. Almeida, Paulo C. F. Mathias
  Abstract: Post-natal overfeeding leads to development of obesity and metabolic dysfunction. PPARα
  activation improves steatosis and metabolic dysfunction associated with obesity. Thus, we
  have hypothesized that PPARα would prevent the metabolic disorders and obesity caused by
  litter reduction. Wistar male offspring rats were divided into normal litter (NL - 9 pups) and
  small litter (SL - 3 pups) and were daily treated with an PPARα agonist, fenofibrate, from
  post-natal day (PN) 1 until weaning (PN21), with either, fenofibrate (F - 12.5mg/kg) or
  vehicle (V). Forming the following groups: NL-V, NL-F, SL-V, SL-F. At PN120 animals
  were euthanized and histological evaluation of samples from liver, visceral (VAT),
  subcutaneous (SAT) and brown (BAT) adipose tissue were performed. Fenofibrate was able
  to prevent the development of microsteatosis in the liver caused by postnatal overfeeding. In
  regard to VAT, there was no difference in the mean adipocyte area, however in distribution
  analyzes it was observed higher frequency of larger adipocytes in SL groups, and no
  difference was observed in SAT. SL-V animals had increased lipid area in BAT, which was
  reduced in SL-F. Increased PPARα activation induced by an agonist during lactation
  improved some histopathological abnormalities induced by postnatal overfeeding. This was
  proven by the decrease in microsteatosis in the liver and improvement in VAT and BAT
  morphology, thus implying a better metabolic health.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68208
  Issue No: Vol. 27, No. ESPECIAL (2023)
VITAMIN D SUPPLEMENTATION DECREASES THE BODY FAT STORES AND PLASMA
OXIDATIVE STRESS OF OBESE RATS
- Authors: Maiara Mikuska Cordeiro, Gustavo Henrique de Souza, Evelyn Silva Moreira, Any Carolina Chagas Almeida, Sabrina Rodrigues Vichetti, Dionízia Xavier Scomparin, Jurandir Fernando Comar
  Abstract: The study aimed to verify the effects of vitamin D3 (cholecalciferol) supplementation on the
  body fat composition and plasmatic oxidative stress of obese rats. The 21 days old male
  Wistar rats were acclimatized in the sectorial animal breed until they were 30 days old
  (CEUA nº 5866200720). Then the obesity induction was initiated using the cafeteria diet
  (Western Diet; WD). The animals were divided into 2 groups: CTL, rats fed a normal diet;
  WD, animals fed a WD. After 90 days old, they were subdivided into two other groups: CTL-
  VD and WD-VD, which received vitamin D3 during 40 days (5.600 UI/week by gavage). At
  130 days old, blood was collected and the plasma separated for the oxidative stress assays.
  After, body fat deposits were collected, separated and weighed. The adiposity index of WD
  rats were higher than CTL and WD-VD animals. The epididymal, retroperitoneal and
  mesenteric fat deposits of WD were respectively 30%, 46% and 50% higher in WD rats when
  compared to WD-VD rats. Body weight gain and abdominal fat were also decreased in
  animals supplemented with vitamin D3. Regarding the oxidative stress parameters in the
  plasma, the levels of carbonyl protein groups were lower, and protein thiol groups and total
  antioxidant capacity (TAC) were higher in WD rats when compared to the CTL and WD-VD
  groups. In conclusion, treatment with VD3 was able to reduce both fat stores and the levels of
  oxidative stress markers in the plasma of rats with cafeteria diet-induced obesity.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68209
  Issue No: Vol. 27, No. ESPECIAL (2023)
EFFECT OF CHOLECALCIFEROL SUPPLEMENTATION ON HEPATIC STEATOSIS AND GLUCOSE
IN OBESE ANIMALS
- Authors: Maiara Mikuska Cordeiro, Evelyn Silva Moreira, Any Carolina Chagas Almeida, Beatriz Paes Silva, Carla Indianara Bonetti, Dionízia Xavier Scomparin, Jurandir Fernando Comar
  Abstract: Our proposal in this study is that with vitamin D supplementation in obese animals, there will
  be reductions in hepatic steatosis and control of blood glucose levels. Male wistar rats were
  used (CEUA nº 5866200720). After 21 days of age, they were acclimatized until they were 30
  days old, from this date on, the obesity induction protocol was initiated, by means of the
  cafeteria diet. The animals were divided into 2 groups: rats fed a normal diet [control group
  (CTL n= 24)] another with a hypercaloric diet [obesity induction group (WD n=24)]. After 90
  days old, they were subdivided into two other groups: CTL-VD (n=12) and WD-VD (n=12)
  animals in which, were supplemented with vitamin D3 (5.600UI/week, 90 to 130 days old, by
  gavage). At 131 days old, WD rats exhibited a significantly higher adiposity index than CTL
  and WD-VD rats. The WD-VD rats had a significantly lower adiposity index than WD rats.
  The hepatic steatosis was confirmed by biochemical measurements of the total liver lipid
  content increased in WD rats 5% of the liver weight. Vit D3 supplementation decreased BW
  gain, and reduction of the total lipid total liver in WD-VD rats. The glycemic curve was
  performed and the WD animals showed higher values in the first 15 minutes than the CTL
  animals. The WD-VD animals presented normal values of the area under the curve, in relation
  to the WD. Vit D supplementation showed that there is a relationship between increased
  adipose tissue and circulating vitamin D levels. Supplementation of VD3 attenuated hepatic
  steatosis.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68210
  Issue No: Vol. 27, No. ESPECIAL (2023)
METHYLPHENIDATE EXPOSURE DURING PERIPUBERTAL PHASE AFFECTS LEARNING AND
MEMORY OF ADULT RATS
- Authors: Maria Natália Chimirri Peres, Luana Y. Chinen, Scarlett R. Raposo, Willian N. S. Rodrigues, Filipe L. Santos, Gabriel Kian Guimarães Lopes, Letícia Ferreira Barbosa, Letícia Ferreira Barbosa, Marcos Vinícius Martins, Rafael P. Lopes, Humberto Milani, Rubia Weffort de Oliveira, Paulo Cesar de Freitas Mathias, Kesia Palma- Rigo
  Abstract: Methylphenidate (MPH) is the active ingredient in Ritalin, although effective in treating of
  the attention deficit hyperactivity disorder (ADHD), its neurostimulation action is attractive
  among students in order to increase the focus or improving student performance. In context of
  the Developmental Origins of Health and Disease (DOHaD) concept, states that insults in
  critical phases of development, as the peripubertal period, may program the organism to
  dysfunctions in adulthood. The aim of this work was to investigate the impact of peripubertal
  MPH exposure on learning and retrograde memory in adult rats. Male Wistar rats were
  exposed to MPH (5 mg/kg) from post-natal day (PN) 30 until PN60 (MPH). Control animals
  received 0.9% saline (Sal). At PN60, learning was evaluated true 10 days in an eight-arm
  elevated maze and at PN120 retrograde memory. At PN60, MPH group showed a delay of
  61% in the latency, an increase of 100% in the number of errors and 200% in the number of
  repetition errors when compared to the Sal group, until the day 5 of learning period (p=0.004).
  After the washout period, at PN120, the MPH group showed an increase of 37% in the
  latency, 49% in the number of errors of reference and 600% in the number of repetition errors
  compared with the Sal group (p=0.001, p=0.004 and p=0.01). Exposure to MPH during
  peripubertal phase decreases learning and memory in adult male Wistar rats.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68211
  Issue No: Vol. 27, No. ESPECIAL (2023)
HYDROETHANOLIC EXTRACT OF TAMARINDUS INDICA SEEDS DECREASES BODY AND LIVER
FAT IN OBESE MICE
- Authors: Gustavo Henrique de Souza, Beatriz Paes Silva, Maiara Mikuska Cordeiro, Vinicius Franco de Oliveira, Lívia Bracht, Jurandir Fernando Comar, Rosane Marina Peralta, Adelar Bracht, Anacharis Babeto de Sá-Nakanishi
  Abstract: Obesity is a metabolic disorder related to several comorbidities. Recent studies found that the
  hydroethanolic extract of Tamarindus indica seeds inhibits the absorption of sugar and fat in
  the intestine. This work aimed to evaluate the effect of this extract on the fat weight and liver
  lipid profile of obese mice induced by a high-calorie diet. 21-day-old male Swiss mice were
  divided into control (standard diet) and cafeteria (high-calorie diet) groups. After certifying
  that cafeteria groups were glucose intolerant (90 days after the onset of high-calorie diet),
  some obese animals were orally treated (gavage) with the hydroethanolic extract of T. indica
  seeds (500 mg/Kg) for 30 days. The animals were euthanized, and the liver and the body fats
  were collected. Obese animals showed an increase in all fats' weight and liver lipid content.
  Treatment decreased epididymal (47%), retroperitoneal (55%), mesenteric (27%), and brown
  (14%) fat weight. In addition, the extract of T indica seed reduced the levels of triglycerides
  (42%) and total cholesterol (30%) in the liver. We conclude that hydroethanolic extract of T.
  indica seeds can be a potential preparation for treating and management of obesity.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68212
  Issue No: Vol. 27, No. ESPECIAL (2023)
THE EFFECT OF BOLDINE ON BODY COMPOSITION AND CARBOHYDRATE METABOLISM IN
OBESE MICE
- Authors: Beatriz Paes Silva, Gustavo Henrique de Souza, Maiara Mikuska Cordeiro, Vinicius Franco de Oliveira, Lívia Bracht, Jurandir Fernando Comar, Rosane Marina Peralta, Adelar Bracht, Anacharis Babeto de Sá-Nakanishi
  Abstract: Boldine is an alkaloid widely found in boldo leaves. Recent studies have determined its
  potential effect on glycemic and lipidemia control. Because of this, the objective of this work
  was to evaluate the effect of boldine on body composition and carbohydrate metabolism in
  obese mice induced by a high-calorie diet. 21-day-old male Swiss mice were divided into
  control (standard diet), cafeteria (high-calorie diet) and treatment (boldine 20mg/Kg) groups.
  After 90 days of treatment, the animals were euthanized, the liver was collected, and
  epididymal, retroperitoneal, mesenteric and brown fats were weighed. Obese animals
  increased body and fat weight, but boldine treatment decreased body weight (17%) and
  abdominal circumference (15%) and reduced retroperitoneal (66%) and mesenteric (33%) fat
  weight. Obesity altered the activity of glucokinase and glucose 6-phosphatase in the liver.
  However, the treatment decreased glucose 6-phosphatase activity (30%) and increased
  glucokinase activity (43%). We conclude that boldine can be a potential preparation for the
  management and treating obesity.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68213
  Issue No: Vol. 27, No. ESPECIAL (2023)
METFORMIN DURING ADOLESCENCE ATTENUATES METABOLIC MARKERS IN ADULT RATS
OVERFED DURING LACTATION
- Authors: Gabriel Tezolim, Veridiana Mota Moreira, Paulo Cezar de Freitas Mathias
  Abstract: The growing prevalence of obesity around the world requires a better understanding of this
  pathophysiology and strategies to mitigate its deleterious effects. Obesity, related to lifestyle,
  is also associated with the DOHAD concept or ontogenetic plasticity. On the other hand, the
  administration of certain drugs in critical periods of development can inhibit or reverse this
  situation. This study aims to evaluate the effects of metformin treatment during adolescence
  on the metabolism of overfed rats during lactation. For this purpose, the litters were
  standardized in 9 pups until the 3rd day of life, and later adjusted in Normal Litters (NL, 9
  pups), and Reduced Litters (SL, 3 pups). At 30 days of age, they were subdivided into 4 new
  groups: NL-saline gavage (NL-S), NL-metformin gavage (NL-M), SL-saline gavage (SL-S)
  and SL-metformin gavage (SL-M). Metformin treatment (300 mg/kg body weight) was given
  daily during adolescence (PN30-PN60). At the end of lactation (PN21), SL animals presented
  a greater body mass and abdominal size. In contrast, at 120 days old, SL-M rats exhibited
  better peripheral insulin sensitivity, oral glucose tolerance, visceral adiposity, and lipid profile
  compared to SL-S rats. Metformin did not alter the metabolism of NL animals. In conclusion,
  treatment with metformin during adolescence, in rats induced obesity by the litter reduction
  model, was effective in attenuating signs of obesity and/or metabolic syndrome, as well as
  restoring the glycemic homeostasis of these animals to levels similar to equivalent controls.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68214
  Issue No: Vol. 27, No. ESPECIAL (2023)
AEROBIC EXERCISE TRAINING DURING ADOLESCENCE REVERSES THE METABOLIC
DYSFUNCTION INDUCED BY THE LITTLE REDUCTION MODEL
- Authors: Filipe Lima Santos, Maria N. C. Peres, Scarlett R. Raposo, Willian N. S. Rodrigues, Camila B. Zara, Leticia F. Barbosa, Kerolym L. Cruz, Douglas L. Almeida, Veridiana M. Moreira, Paulo C. F. Mathias
  Abstract: Sedentary lifestyle and consumption of high-calorie foods contribute to the development of
  individuals with risk factors for non-communicable diseases. Aerobic exercise is one of the
  main non-pharmacological strategies in this confrontation. This work aims to evaluate the
  effects of moderate-intensity exercise (MIE) on biometric and metabolic parameters of adult
  male Wistar rats, programmed for obesity during the lactation period. The litters were
  standardized in 9 pups until the 3rd day of life, and later adjusted in Normal Litters (NL), and
  Reduced Litters (SL). At 21 days, the offspring were weaned and divided into sedentary
  (SED) and exercised (EXE) groups, forming 4 experimental groups: NL-SED, NL-EXE, SL-
  SED and SL-EXE. EXE rats performed a moderate-intensity treadmill running protocol from
  30 to 60 years old. All groups were euthanized at 120 days. The results showed that the litter
  reduction made the animals heavier (p<0.0001), hyperphagic (p<0.001), greater mesenteric fat
  stock (p<0.0001), insulin resistant (p=0.0001) and glucose intolerant (p=0.0001), when
  compared to equivalent controls. MIE promoted a reduction in body weight (p=0.001) and
  mesenteric fat (p<0.0001), better insulin sensitivity (p=0.01) and glucose tolerance (p=0.003).
  It is concluded, therefore, that MIE performed in adolescence attenuates the deleterious
  effects observed on body composition and glucose metabolism in adult animals, programmed
  early for obesity.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68215
  Issue No: Vol. 27, No. ESPECIAL (2023)
METFORMIN DURING ADULTHOOD CANNOT ATTENUATE THE HARMFUL EFFECTS OF
NEONATAL OVERNUTRITION
- Authors: Rafael Pereira Lopes, Scarlett Rodrigues Raposo, Lucas Paulo Jacinto Saavedra, Marcos Vinícius Martins, Kérolym Lomes da Cruz, Letícia Ferreira Barbosa, Maryana Debossan Fernandes, Maria Natália Chimirri Peres, Mariana Narciso, Paulo Cezar de Freitas Mathias
  Abstract: Under- or overnutrition, during critical periods of development, lead to the development of
  obesity and cardiometabolic dysfunction later in life in rats that have been programmed for
  the development of metabolic dysfunction in a litter reduction model. On day 1, all litters are
  standardized to 9 pups per dam and on day 3 after birth, litters were adjusted to 3 pups per
  dam in the small litter group (SL) and the NL group remained with 9 animals. At 70 days of
  age, the animals were separated into 2 new groups: Saline (SAL) and Metformin (MET). For
  12 days, the NL and SL animals were treated daily with saline, giving rise to the NL-SAL and
  SL-SAL groups, or were treated with Met 100mg/kg/day, giving rise to the NL-MET and SL-
  MET groups. The treatment ended at 82 days of age and the animals were taken up to 142
  days of life. Body weight remained significantly higher in SL compared to NL animals up to
  142 days (p<0.0001), with no significant difference in relation to treatment. And the same
  was observed in relation to glucose intolerance and insulin resistance (p<0.0001). We can
  conclude that short-term treatment with metformin did not attenuate the metabolic
  dysfunction induced by neonatal overnutrition.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68217
  Issue No: Vol. 27, No. ESPECIAL (2023)
AEROBIC EXERCISE PERFORMED IN ADULT LIFE MAINTAINS BODY WEIGHT AND FOOD
INTAKE OF MALE RATS EVEN AFTER TRAINING INTERRUPTION
- Authors: Marcos Vinícius Martins, Leticia Ferreira Barbosa, Scarlett Rodrigues Raposo, Maria Natalia C. Peres, Gabriel Kian G. Lopes, Maryana D. Fernandes, Kerolym L. da Cruz, Rafael P. Lopes, Veridiana Mota Moreira, Paulo Cezar de Freitas Mathias
  Abstract: Excess weight in adult life is an important risk factor for the development of
  noncommunicable diseases. On the other hand, aerobic physical exercise programs have been
  shown to be important components both in the prevention and control of these conditions.
  This work aims to evaluate the effects of moderate intensity training on the body weight
  (BW) and food intake (FI) of adult male rats. Wistar rats were obtained at 30 days old.
  Throughout the experimental period, they were kept in appropriate cages (4 rats per box)
  containing food and water, temperature (22ºC) and photoperiod (07:00 to 19:00) controlled.
  At PN 40 they were divided into sedentary (S) and exercised (E) groups. The BW and FI were
  measured twice a week, from 40 to 140 days old. Exercised rats performed a moderate-
  intensity treadmill running from PN 90 to 120. During this period, they were submitted to an
  effort test to evaluate performance and control the training load (55% to 65% VO 2max ). The
  results showed that both BW and FI were not altered with the training performed during adult
  life, even after 20 days of exercise cessation. More studies are needed in order to assess
  whether moderate-intensity training is able to attenuates the deleterious effects observed in
  the biometric and metabolic markers of adult rats programmed for obesity and/or metabolic
  syndrome.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68219
  Issue No: Vol. 27, No. ESPECIAL (2023)
METABOLIC DYSFUNCTIONS CAUSED BY EARLY FRUCTOSE INGESTION ARE PREVENTED IN
CONCURRENTLY EXERCISED ADULT MALE RATS
- Authors: Letícia F. Barbosa, Sandra S. Silveira, Christian M. Mendes, Veridiana M. Moreira, Paulo C. F. Mathias
  Abstract: This study evaluated whether fructose ingestion at adolescence programs the development of
  metabolic syndrome (MetS) in adult rats and whether moderate-intensity combined exercise
  can attenuate fructose-induced MetS compounds. Pubertal rats were trained on a treadmill
  (55-65% VO 2max ), 3 times/week, 44 min/session, for one month. They were allocated into four
  groups: sedentary control (SC), trained control (TC), sedentary fructose (SF), and trained
  fructose (TF). Groups F ingested 10% fructose. Food intake (AI) and body weight (BW) were
  measured weekly. At 60 and 120 days of age, metabolic parameters were evaluated. Fructose
  intake mainly affected animals in adult life, and these changes were related to glucose
  intolerance (p=0.001), periepididymal fat (p<0.03), and increase in total cholesterol (TC) (p
  <0.001), triglycerides (TAGs) (p<0.0001) and insulin (p<0.0001) levels. Early physical
  exercise was able to decrease periepididymal (p<0.003) and retroperitoneal (p<0.0002)
  adipose tissue, TC levels (p<0.0001) and glucose tolerance (p=0.21). Rats that received
  combined treatments during adolescence showed low glycemia (p=0.003) and insulinemia
  (p<0.0001) during the intraperitoneal glucose tolerance test (ipGTT). HOMA-IR (p=0.02) and
  TYG (p=0.0003) scores also improved in these rats. We conclude that early ingestion of 10%
  fructose was effective in programming adult rats to MetS, and simultaneous moderate
  exercise attenuated these aggressions, causing changes in glycemic homeostasis and lipid
  metabolism.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68221
  Issue No: Vol. 27, No. ESPECIAL (2023)
EARLY METFORMIN TREATMENT IMPROVES GLUCOSE TOLERANCE IN OVERFED LACTATING
RATS
- Authors: Milene A. Bobato, Maiara V. G. Ribeiro, Maria N. C. Peres, Lucas P. J. Saavedra, Silvano Piovan, Gessica D. Gonçalves, William N. S. Rodrigues, Scarlett R. Raposo, Ananda Malta, Paulo C. F. Mathias
  Abstract: Metformin is the most widely used drug for the treatment of type 2 diabetes and metabolic
  diseases worldwide. Lactation is understood as a sensitive phase of development capable of
  programming the metabolism for health or disease in adult life. The aim of this study was to
  verify whether treatment with metformin during lactation could attenuate the metabolic
  dysfunctions in Wistar rats induced obesity by overfeeding during lactation. At birth, all
  litters were adjusted to 9 pups (NL) for each mother and divided into two experimental
  groups: NL: Normal Litter Saline, NL-M: Normal Litter Metformin. Small Litter (SL) litters
  were reduced to 3 pups per mother on the third day of life and were divided into two
  experimental groups: SL: Small Litter Saline and SL-M: Small Litter Metformin. Salina (S)
  pups received 0.9% saline solution from the first to the twelfth day of life via intraperitoneal
  injection. The other group received metformin (M) (100mg/kg of body weight (bw)/day) in
  the same period of life. Early metformin treatment decreased body weight gain (BW) and
  food intake (FI) in SL-M rats at 90 days old. The increase in retroperitoneal, mesenteric and
  gonadal fat reserves in SL animals not treated with metformin was shown to be reduced in
  treated SL animals. The intravenous glucose tolerance test showed greater tolerance in SL-M.
  We conclude that early treatment with metformin prevented BW gain and visceral adiposity
  in adult rats, caused by overfeeding during lactation, as well as improved glucose tolerance, a
  parameter also altered in small litter animals.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68222
  Issue No: Vol. 27, No. ESPECIAL (2023)
POTENTIAL ANTIDIABETIC OF TAMARIND WASTE: EFFECTS ON THE PANCREATIC
-AMYLASE
- Authors: Driele Cardoso Bueno, Gustavo Henrique de Souza, Beatriz Paes Silva, Rosane Marina Peralta, Lívia Bracht, Jurandir Fernando Comar, Adelar Bracht, Anacharis Babeto de Sá-Nakanishi
  Abstract: Diabetes is a complex metabolic disease characterized by plasma hyperglycemia. One of the
  ways of treatment of diabetes is the inhibition of pancreatic α-amylase. However, drugs
  available in the market carry out a range of side effects. Therefore, the present work aimed to
  evaluate the effect of tamarind waste (skin and seed) rejected by the food industry on the
  activity of pancreatic α-amylase. Hydroethanolic extract (70%) of tamarind skin and seed
  were prepared, lyophilized, and used in a concentration curve on the α-amylase activity by 3,5
  dinitro-salicylate assay. Kinetic analysis was performed simultaneously varying both substrate
  (starch) and extracts. Statistical analyses were carried out by Scientist Program. Both extracts
  (skin and seed) reduced the enzymatic activity in a concentration dependence way. However,
  the seed extract reported a bigger strength of inhibition, as revealed by IC 50 of 13,26 µg/mL,
  compared to IC 50 of 470,16 µg/mL for skin extract. Kinect studies showed that the seed
  extract presented an enzymatic inhibition of parabolic and non-competitive type (KI 1 10,07
  µg/mL e KI 2 10,37 µg/mL). Conversely, skin extract performed a mixed linear inhibition (KI 1
  615,78 µg/mL e KI 2 411,01 µg/mL). We concluded that tamarind waste, mainly seed extract,
  can be considered preparation with therapeutic potential for the management and treatment of
  diabetes.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68223
  Issue No: Vol. 27, No. ESPECIAL (2023)
DYSLIPIDEMIA CHANGES EXERCISE ENERGY EXPENDITURE IN MICE TRANSGENIC FOR
HUMAN APOCIII
- Authors: Nilton Rodrigues Teixeira Junior, Diogo Rodrigues Jimenes, Cristiany Schultz, Jairo Augusto Berti
  Abstract: Hypertriglyceridemia is a predisposing factor for several metabolic diseases in the world
  today. Their cause is multifactorial, several factors are directly linked to the modification of
  plasma lipid metabolism, among them are genetics and exercise. The genic overexpression of
  apoCIII makes the animal hypertriglyceridemic and high levels of blood cholesterol, enabling
  further studies to be done on its metabolic profile. This study investigates how apoCIII
  overexpression and exercise modify the metabolism of genetically modified mice, especially
  their energy expenditure. We used 15-month-old male C57Bl/6 mice, subdivided into 4
  groups: 2 NTG groups (non-transgenic mice that were submitted to training and their
  controls) and 2 CIII groups (transgenic Mice with basal triglyceridemia above 300mg/dL that
  were submitted to training and their controls). The exercised groups underwent for 8 weeks a
  moderate intensity training for 40 minutes 3 times a week. All animals were submitted to a
  stress test to evaluate performance and energy expenditure. The trained animals had higher
  energy expenditure during all stages of the test (p<0.01) and the area under curve of the EE
  showed that the trained animals had higher caloric consumption (p<0.001), while the animal
  CIII sed had a much higher energy expenditure than its control NTG sed (p<0.001). We
  conclude that dyslipidemia makes the CIII animal less fit for exercise, and trained animals
  have lower energy expenditure independent of dyslipidemia and produced more work.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68224
  Issue No: Vol. 27, No. ESPECIAL (2023)
AGE AND OBESITY INTERFERES IN MUSCLE HYPERTROPHY IN RATS UNDERGOING HIIT
TRAINING
- Authors: Diogo Rodrigues Jimenes, Fernanda Cândido, Nilton Rodrigues Teixeira Júnior, Wilson Rinaldi, Carmem Patrícia Barbosa
  Abstract: Sarcopenic obesity is a condition characterized by the simultaneous presence of obesity and
  sarcopenia, which is the loss of muscle mass and function. Is a growing concern, especially
  among the elderly population, and it is important to address both obesity and sarcopenia
  through lifestyle modifications, such as exercise and proper nutrition, to improve overall
  health and quality of life. Our study aimed to evaluate the effect of HIIT training on the
  muscle fiber area of the EDL muscle in obese rats of different ages. For that, Wistar rats, 7
  and 12 months old, submitted or not to a HFD diet were subjected to HIIT training, 3 times a
  week, on alternate days, for 8 consecutive weeks. After the euthanasia EDL muscles was
  collected and processed histologically. Our data showed that HIIT training was able to
  promote muscle hypertrophy even with consumption of this diet, indicating that practicing
  this exercise can stimulate protein synthesis even under conditions of obesity.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68225
  Issue No: Vol. 27, No. ESPECIAL (2023)
HIGH INTENSITY INTERVAL TRAINING DOES NOT CHANGE THE AREA IN THE
ADIPOCYTES OF THE EPICARDIUM OF FAT RATS
- Authors: Fernanda Candido, Daniel Costa Gonçalves do Vale;, Diogo Rodrigues Jimenes, Nilza Cristina Buttow, Carmem Patrícia Barbosa
  Abstract: The obesity due to high caloric ingestion is related to the accumulation of body fat and
  predisposes cardiovascular diseases. Recent studies about the High Intensity Interval Training
  (HIIT) evidence benefits of its practice in patients that maybe carrying this disease, mainly
  because of the reduction of comorbidities associated with obesity. This present study has the
  objective of evaluating the HIIT effects on the adipocytes’ changes of the epicardium adipose
  tissue in fat animals induced by the consumption of high-fat diet. It was used male rats of
  Wistar lineage fed daily with HFD and trained by HIIT three times a week for 8 consecutive
  weeks. The animals were weighted during the training sections and, after its euthanasia, were
  realized histological analyses of the epicardium adipose tissue. The results showed that HFD
  increased the body weight of the animals and the side view of the adipocytes. However, the
  HIIT did not change the area of the adipocytes of the epicardial adipose tissue, even though
  the diet had been a hypertrophy factor of this tissue. Then, we can conclude that HIIT
  contributes to avoid the body weight gain but was not capable of reducing the area of the
  adipocytes of the epicardium adipose tissue.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68226
  Issue No: Vol. 27, No. ESPECIAL (2023)
GHSR MODULATION BY LEAP2 IN PERINATAL PHASES IMPACTS GLUCOSE OVERLOAD
RESPONSIVENESS IN YOUNG RATS
- Authors: Marcos Divino Ferreira-Junior, Keilah Valéria Naves Cavalcante, Ariel Penha Carvalho da Mota, Maria Eduarda Ribeiro da Silva, Isadora Silva Rosa, Jose Antonio Barbosa Lima Sobrinho, Carlos Henrique Xavier, Paulo Matafome, Rodrigo Mello Gomes
  Abstract: GHSr signaling is involved in the regulation of energy homeostasis. LEAP2, a recently
  discovered peptide hormone, counteracts the effects of ghrelin. This study sought to evaluate
  the effects of perinatal LEAP2 exposure in young rat offspring. For this, two protocols were
  designed to assess the roles of GHSr antagonism during the pregnancy and during the
  lactation. Sex-specific impacts on AUC and peak-responses of glucose tolerance tests were
  presented. However, no differences were detected in the blood glucose of mixed meal fed
  animals. In conclusion, perinatal LEAP2 exposure could impact the glucose-stimulated
  insulin secretion in young rats.
  PubDate: 2023-05-11
  DOI: 10.4025/arqmudi.v27iESPECIAL.68227
  Issue No: Vol. 27, No. ESPECIAL (2023)
CHRONIC EFFECT OF HYDROGYM IN PEOPLE WITH OBESITY AND CNCD POST-COVID: A
PILOT STUDY
- Authors: Andressa Aparecida de Souza, Wesley Gabriel da Silva Alexandrino, Thais Regina de Sousa, Mara Jane dos Santos Pascoini, César Faúndez Casanova, Nelson Nardo Junior
  Abstract: The objective of this study is to verify the effec of the practice of Hydrogymnastics as a
  regulatory factor for the treatment of obesity and post-COVID CNCDs. This is a descriptive
  research with a comparative approach, carried out with individuals with obesity. A 16-week
  Intervention program was applied, in which 40 volunteers participated, who were assigned to
  an Intervention Group (IG) and a Comparison Group (CG). The analysis was descriptive
  between pre and post test (GI= 26 and GC=14). Normality was contrasted for the total
  sample, and by group (G1 and G2) for each of the measured variables. In the intervention
  group, some variables were maintained and did not change significantly, there were
  improvements only in DBP and HR. In the CG there were improvements only in the HR
  variable, they also obtained improvements in weight, BMI, DBP and SBP, with a small effect
  size. The Hydrogymnastic Training Program, performed in adults with obesity, in this study
  showed an improvement in the response of the double product: heart rate and systolic blood
  pressure.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68175
  Issue No: Vol. 27, No. ESPECIAL (2023)
EFFECTS OF SWIMMING TRAINING IN OBESITY INSTALLATION IN ADULTS OFFSPRING
BORN WITHIN A SMALL LITTER
- Authors: Bruna Schumaker Siqueira, Stefani Valéria Fischer, Amanda Rocha Fujita, Sabrina Grassiolli
  Abstract: The excessive supply of energy during pregnancy and breastfeeding period, favors the high
  accumulation of white adipose tissue (WAT) throughout life, commonly associated with
  insulin resistance (IR), dyslipidemia and cardiovascular disease in adulthood. Chronic aerobic
  exercises reduce accumulation of WAT, preventing the development of metabolic diseases.
  On the 2 nd postnatal day, litter size was set to: normal (NL) with 9 pups per mother and small
  (SL) with 3 pups per mother, was used just male rats. After weaning (21 days), NL and SL
  rats, were divided into sedentary (Sed) and exercised (Exe), forming 4 experimental
  subgroups; NL Sed , NL Exe , SL Sed and SL Exe . From 22–90 days of life exercised groups performed
  swimming training 3 times for week during 30 minutes. Litter handling influenced body
  weight, retroperitoneal and mesenteric-WAT (p<0.0001) in adulthood. SL Sed animals showed
  an increase in these parameters when compared to NL Sed and NL Exe animals (P<0.0001).
  Swimming training reduced body weight and WAT deposits in NL and SL groups
  (P<0.0001). SL Sed and SL Exe animals showed elevated glucose values at 2h post-glucose load
  when compared to NL animals. Kitt was also altered by litter reduction (P<0.0001), with SL Sed
  animals had higher Kitt values compared to NL Sed and NL Exe rats. This response was
  normalized in SL Exe group.We conclude that lactational hypernutrition causes obesity
  associated with disruption of glucose homeostasis and swimming training normalizes insulin
  sensitivity but is insufficient to restore glucose homeostasis during glucose tolerance test.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68176
  Issue No: Vol. 27, No. ESPECIAL (2023)
PROTEIN-CALORIC RESTRICTION DURING LACTATION HAS A TRANSGENERATIONAL
EFFECT IN GLUCOSE HOMEOSTASIS OF MALE RATS
- Authors: Emanuelle Viana, Audrei Pavanello, Paulo Cezar de Freitas Mathias, Isabela Peixoto Martins
  Abstract: Experimental and epidemiological studies demonstrated an important association between
  insults in sensitive stages of development and metabolic syndrome at adulthood.
  Developmental Origins of Health and Disease (DOHaD) concept indicates that pregnancy and
  lactation are windows to metabolic programming, once in these phases, occurs a peak of
  neuronal development. There is a gap in the study of the transgenerational effect of insults
  performed in programming windows, especially when it comes to malnutrition. Female
  Wistar rats were submitted to a protein-caloric restriction during the first 14 days of lactation
  (LP, low-protein group) or a normal-caloric diet through the lactation (NP, normal-protein
  group). At 70 days old these females were mated with a control male rat. The offspring (F2
  generation, NP and LP groups) were fed with normal-caloric diet and were euthanized at 21
  days old to blood collection and biometric analysis. There were no differences between
  groups in body weight at weaning. Also, retroperitoneal, periepididymal and mesenteric fat
  pads were similar between NP and LP animals. We showed an 11% (P˂0.05) increase in
  brown adipose tissue weight in LP group. Fasting glucose was increased by 99% (P˂0.05) in
  LP groups, without alteration in fasting insulin levels. We conclude that protein-caloric
  restriction during lactation has a transgenerational effect in male rats, altering the glucose
  homeostasis.
  PubDate: 2023-05-10
  Issue No: Vol. 27, No. ESPECIAL (2023)
IDENTIFICATION OF CRITICAL LACTATION STAGE FOR THE BODY COMPOSITION IN
MALE OFFSPRING OF HIGH-FAT FED DAMS
- Authors: Maryana Debossan Fernandes, Ananda Malta, Letícia Ferreira Barbosa, Scarlett Rodrigues Raposo, Marcos Vinícius Martins, Gabriel Kian Guimarães Lopes, Douglas Lopes Almeida, Paulo Cezar de Freitas Mathias
  Abstract: Each stage (early, middle and late) of lactation is a critical period during which maternal
  malnutritional can negatively effects on the offspring's body development. We investigated
  the effects of a maternal high fat diet (HF) during the different stages of lactation on body
  weight of male offspring at weaning. Female Wistar rats were fed either a HF diet only during
  the first week (HF1-7 group), second week (HF7-14 group), third week (HF14-21 group) and
  throughout lactation (group HF1-21) or standard diet during all lactation. Biometric
  parameters of dams and male offspring were evaluated. HF fed dams did not have a difference
  in body weight, food intake and fat pad compared with the control dams. Regarding the
  offspring, a higher final body weight was observed in males from the HF7-14 and HF14-21
  and HF21 groups compared to the control. There was an increase in retroperitoneal and
  mesenteric fat in HF21 and HF14-21 compared with control groups. Higher perigonadal fat
  pat was only observed in HF21. Consumption of maternal HF diet throughout lactation causes
  high body weight in male offspring. Interestingly, each isolated phase also causes important
  body weight accumulation, mainly the second and third week.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68178
  Issue No: Vol. 27, No. ESPECIAL (2023)
GLYCEMIC HOMEOSTASIS OF SMALL AND NORMAL LITTER DURING RAT ADOLESCENCE
- Authors: Scarlett Rodrigues Raposo, Lucas Paulo Jacinto Saavedra, Maria Natália Chimirri Peres, Rafael Pereira Lopes, Marcos Vinícius Martins, Filipe Lima dos Santos, Willian Nascimento de Souza Rodrigues, Maryana Debossan Fernandes, Mariana Narciso Constancio, Gabriel Kian Guimaraes Lopes, Letícia Ferreira Barbosa, Kérolym Lomes da Cruz; , Ananda Malta, Paulo Cezar de Freitas Mathias
  Abstract: Childhood obesity has become a public health concern, as it leads to cardiometabolic
  disorders. The Developmental Origins of Health and Disease (DOHaD) concept shows that
  insults in critical windows of development can program the individual for both health and
  disease. In the neonatal supernutrition model, known as small litter (SL), the litter is reduced
  to 3 animals per dam, mimicking obesity, while the normal litter (NL) control animals have
  only 9 animals per dam. The period of adolescence in SL animals is still little explored, it is
  known that in this phase there are many hormonal changes, but it is still not known how the
  behavior is in relation to glycemia, so our objective was to evaluate glycemia during the
  tolerance test oral glucose in NL and SL animals during adolescence. It is observed that the
  NL and SL animals show no difference in glucose tolerance at 45 and 60 days. However, both
  groups are glucose intolerant when compared to their respective controls in adult life. We
  conclude that there is glucose intolerance during adolescence regardless of the nutritional
  insult.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68179
  Issue No: Vol. 27, No. ESPECIAL (2023)
IMPACT OF PERINATAL OVERFEEDING ON BODY MASS OF FEMALE WISTAR RATS
- Authors: Mariana Narciso Constancio, Scarlett R. Raposo, William N. S. Rodrigues, Maria N. C. Peres, Marcos V. Martins, Rafael P. Lopes, Kérolym L. Cruz, Paulo Cesar de Freitas Mathias, Douglas Lopes Almeida
  Abstract: Childhood obesity is an important independent risk factor for later metabolic diseases, such as
  type 2 diabetes, dyslipidemia, and arterial hypertension. However, males and females
  respond differently to this nutritional challenge. Here, we propose an experimental study of
  the effects of early obesity, using the litter reduction model, in the body mass of female adult
  Wistar rats. The litters were delivered on postnatal day 0 - PN0. In PN2, the offspring of
  Wistar rats was standardized in 9 (normal litter – NL) and 3 (reduced litter – SL) pups.
  Animals were weaned at PN21 and kept at 3 per cage for both groups until PN120. In PN120,
  the rats were weighed and euthanized, and then the tissues were collected, according to the
  approval of the Ethics in Animal Use Committee n° 4831020822. Our data points to a body
  weight difference in PN21 between the groups, where the SL group was overweight compared
  to the NL group (p<0.0001). In PN120, the SL group continued with a higher weight than the
  NL group (p <0.01). SL rats showed increased weight in periovarian, periuterine,
  retroperitoneal, mesenteric and brown fat (p=0.002, p<0.0001, p=0.001, p=0.002 and
  p=0.0001, respectively) compared to NL animals. Taken together, the present data shows that
  females Wistar rats are vulnerable to the effects of early in life obesity on body mass, leading
  to a greater predisposition to later risk factors associated with obesity.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68180
  Issue No: Vol. 27, No. ESPECIAL (2023)
HOW THE PAST SHAPES CURRENT STORIES: (EPI)GENETIC EPIDEMIOLOGICAL PROFILES
OF METABOLIC SYNDROME IN BRAZILIAN MENNONITES
- Authors: Ana Luiza Sprotte Mira, Kathleen Lietke Kolb, Larissa Schneider, Eduardo Delabio Auer, Priscila Ianzen dos Santos, Guilherme Luiz da Rocha, Valéria Bumiller Bini, Gabriela Canalli Kretzschmar, Sheila Coelho Soares Lima, Angelica Beate Winter Boldt
  Abstract: Mennonites present cc. 500 years genetic isolation and three bottleneck events that reduced
  their genetic diversity. In order to identify (epi)genetic markers for metabolic syndrome (MS),
  we used a modified version of the Brazilian National Health Survey to interview 762
  Mennonites from three settlements, between 2016-2023. We compared 63 vs. 127 exomes
  (Illumina HiSeq) from Mennonites with/without metabolic syndrome (MS) and genotyped
  candidate variants in regulatory regions with mass spectrometry (iPLEX) and sequence-
  specific amplification (PCR-SSP). We also evaluated DNA methylation of the NR3C1 and
  FKBP5 genes in peripheral blood mononuclear cells (PBMCs) of up to 66 and 141 individuals
  with/without MS. MS prevalence was 14.02%, less than half the one reported for
  Neobrazilians (34.8%, P<0.00001), being paralleled by a three times lower prevalence of
  acute myocardial infarction (AMI). Among independent MS risk factors, we found lower
  maternal warmth in infancy (OR=1.59, P=0.019) and a higher susceptibility to AMI with the
  harshest migratory route to Brazil (OR=1.57, P=0.001). Thirty-nine variants of 34 genes were
  associated with MS (p<0.02), 41% create/disrupt CpG sites and 12 were associated with
  visceral adipose and/or cardiovascular tissue expression. There were no methylation
  differences between individuals with and without MS in PBMCs with the NR3C1 and FKBP5
  genes, pointing to other epigenetic causal effects. In conclusion, Mennonites have a peculiar
  epidemiological profile marked by (epi)genetic founder effects that also affect their
  metabolism, calling for urgent action for prevention and to alleviate the burden of
  comorbidities due to late diagnosis.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68181
  Issue No: Vol. 27, No. ESPECIAL (2023)
IMPACT OF THE TYPE OF DELIVERY ON GROWTH, BODY COMPOSITION AND MOTOR
PERFORMANCE OF CHILDREN AND YOUNG PEOPLE
- Authors: Diego Bessa Dantas, João Fernando dos Santos, Vinicius Muller Reis Weber, Sandra Aires Ferreira, Marcos Roberto Queiroga
  Abstract: The aim of this study was to investigate the impact of cesarean delivery (CD) and normal
  delivery (ND) on growth, body composition and motor performance of children and young
  people. The study included 114 children and young people (11 to 18 years old) who were
  divided into two groups (ND and CD). Participants underwent a battery of anthropometric
  measurements and motor tests. Children and young people born with CD showed greater
  performance in the trunk elevation test in extension (flexibility), shorter distance covered (m)
  in the treadmill test (aerobic resistance), greater amount of subcutaneous tissue and
  endomorphy component than children and young people born from ND. It was observed that
  children born with CD in this study had a greater amount of body fat, greater trunk flexibility
  and shorter distance covered on a treadmill, suggesting important advantages for children
  born with ND in relation to obesity and aerobic capacity. These components of physical
  fitness related to health are determinant for the development of chronic degenerative diseases.
  This study reveals that ND should also be encouraged as a health promotion strategy.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68182
  Issue No: Vol. 27, No. ESPECIAL (2023)
LOW PROTEIN DIET DURING PERIPUBERTAL PHASE PROGRAMS TO CARDIOVASCULAR
DYSFUNCTION IN ADULT RATS
- Authors: Anna Rebeka Oliveira Ferreira, Maiara Vanusa Guedes Ribeiro, Guilherme Bernado Cornelio Coelho, Silvano Piovan, Gabriel Kian Guimarães Lopes, Leticia Ferreira Barbosa, Paulo Cezar de Freitas Mathias, Kesia Palma-Rigo
  Abstract: Perinatal malnutrition has a devastation effect later in life. This study evaluated whether
  protein restriction in adolescent rats programs hypertension regarding cardiac dysfunction.
  CEUA approved the experimental protocol under nº 3353060421. 30-day-old Wistar male rats
  were fed a low protein diet (4%) for the next thirty days and subsequently fed a 20.5% normal
  protein diet for the next 60-days with normal diet (LP group). Control rats (NP group) were
  fed a normal protein diet throughout life. Cardiovascular system function was evaluated with
  120-days-old. Statistical analyses were performed with T-Student test. LP animals displayed
  an inferior body mass and nose-tail length (P=0.0001; P=0.01) and also rising retroperitoneal
  fat (P=0.03). LP rats exhibited a higher in mean arterial blood pressure (p=0.05). In the
  echocardiogram, LP group showed an increased in the diameter (p=0.01, p=0.02),
  interventricular septum (p=0.040 e p=0.03) and the in posterior wall (p=0.01 e p=0.03) of the
  left ventricle in diastole and systole respectively. Protein restriction in peripubertal period
  leads to hypertension in adulthood, sustained by greater cardiac dysfunction.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68183
  Issue No: Vol. 27, No. ESPECIAL (2023)
MATERNAL PROTEIN MALNUTRITION DURING LACTATION PREDISPOSES TO A METABOLIC
SYNDROME PHENOTYPE IN MALE WISTAR RATS
- Authors: Willian do Nascimento de Souza Rodrigues, Maria Natalia Chimirri Peres, Scarlett Rodrigues Raposo, Maryana Debossan Fernandes, Marcos Vinícius Martins, Leticia Ferreira Barbosa, Filipe Lima dos Santos, Lucas Paulo Jacinto Saavedra, Veridiana Mota Moreira, Paulo Cezar de Freitas Mathias
  Abstract: Maternal nutritional insults during lactation can modulate the offspring phenotype associated
  with the risk of non-communicable diseases at different stages of ontogenetic development.
  The aim of this research was to analyze biometric and biochemical parameters of male Wistar
  rats, born to dams fed a low-protein diet in the first two-thirds of lactation. Therefore, the
  mothers received ad libitum a normal (NP, 23%) or low-protein (LP, 4%) diet, originating
  two experimental groups that were evaluated at 14 days old (NP-14 and LP-14). The results
  showed that the LP-14 offspring male rats had lower body weight (p<0.0001), reduced liver
  (p<0.0001) and higher brown fat deposits (p<0.0001), compared to the equivalent control.
  They also exhibited hyperglycemia (p<0.05), hypercholesterolemia (p<0.0001), and increased
  serum β-hydroxybutyrate (p<0.0001), contrasting with reduced triglycerides (p<0.05) levels.
  We conclude that maternal exposure to a low-protein diet during lactation was able to
  program 14-day-old male neonates to develop a metabolic syndrome phenotype, probably due
  to restriction of body and visceral growth in these animals.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68185
  Issue No: Vol. 27, No. ESPECIAL (2023)
MATERNAL FOOD RESTRICTION DURING LACTATION LEADS TO SEX-SPECIFIC BLOOD
HYPERTENSION AND CARDIAC REMODELING IN THE OFFSPRING
- Authors: Keilah Valéria Naves Cavalcante, Marcos Divino Ferreira-Junior; , Ariel Penha Carvalho da Mota, Maria Eduarda Ribeiro da Silva, Jose Antônio Barbosa Lima Sobrinho, Rodrigo Mello Gomes, Gustavo Rodrigues Pedrino
  Abstract: Dietary restriction during pregnancy and puberty is a risk factor to hypertension onset at
  adulthood. The aim of this study was to evaluate the effect of maternal malnutrition during
  lactation on cardiovascular parameters, in both sexes of rat pups at 120-day-old. Maternal
  food restriction lead to arterial hypertension, baroreflex impairment, cardiac remodeling and
  increased collagen deposition in the aorta, in adult male offspring. The females did not show
  functional or morphological disruptions. Together, these results show that maternal food
  restriction during lactation promotes cardiometabolic programming, generating cardiovascular
  alterations in male adult rat offspring, however female offspring present some kind of
  cardioprotective effect.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68186
  Issue No: Vol. 27, No. ESPECIAL (2023)
MATERNAL RESTRICTIVE DIETS DURING LACTATION IMPAIR GABAERGIC SIGNALING IN
THE HYPOTHALAMUS AND MIDBRAIN IN MALE ADULT RATS
- Authors: Isadora S. Rosa, Keilah V. N. Cavalcante, Marcos D. F. Junior, Juliana V. V. Ribeiro, Ariel P. C. Mota, Maria E. R. Silva, Jose A. B. L. Sobrinho, Carlos H. Xavier, Gustavo R. Pedrino, Rodrigo Mello Gomes
  Abstract: The aim of this work was to evaluate the behavioral parameters and the GABAergic signaling
  in the hypothalamus, midbrain and medulla. Mothers and litters were distributed into 3
  groups: Control (CO) fed with standard diet (20% protein), food restriction (FR) with
  standard diet limited of 50% of the daily consumption of CO, and low-protein (LP) fed with
  LP-diet (4% protein). Interventions were carried out from the 1st-14th, and 14 th -21st day of
  lactation. Offspring of both sexes were evaluated until 120 days old by elevated plus maze
  (EPM) and open field (OP) test. Male LP rats showed a decrease in the closed arm (CO
  158±11.51 vs LP 105.5±9.63; s), an increase in the open arm (CO 85.10±8.65 vs LP
  139.8±12.36; s) time, and in the number of open arm entries (CO 5.25±0.58 vs LP 8.40±0.79;
  a.u.) compared to the CO. In OP, male LP rats showed an increase in crossings (CO
  51.94±4.29 vs LP 71±3.41; a.u.) and in the frequency of crossings (CO 51.94±4.29 vs
  69.25±4.44; a.u.) compared to the CO. Male FR showed a decrease in the expression of
  GABA B receptors (CO 100±12.60 vs FR 56.34 v 4.19; % control). In the midbrain, male LP
  and FR showed a decrease in GAD (CO 100±11.22 vs LP 47.59±6.21 vs FR 74.18±8.82; %
  control) compared to the CO. In conclusion, maternal protein and caloric restriction during
  lactation induces less anxious behavior and increases exploratory activity in males in
  adulthood, through modulation in GABAergic signaling.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68188
  Issue No: Vol. 27, No. ESPECIAL (2023)
EARLY GLYCOTOXIN EXPOSURE INDUCES EX-VIVO LEFT VENTRICULAR DYSFUNCTION IN
ADULT RATS
- Authors: Ariel P. C. Mota, Maria E. R. Silva, Isadora S. Rosa, Jose A. B. L. Sobrinho, Marcos D. Ferreira- Junior, Keilah V. N. Cavalcante, Jaqueline M. Costa, Carlos H. Castro, Paulo Matafome, Rodrigo M. Gomes
  Abstract: The exposure of the fetus/neonate to glycotoxins via the placenta or breast milk, such as the
  methylglyoxal (MG), has been related to short- and long-term disorders in its development.
  The aim of this study was to evaluate the effects of early exposure to methylglyoxal, during
  the lactation phase, on cardiac parameters at adulthood. All the experimental procedures were
  approved by the ethics committee of animal use of UFG (007/21). Eight pregnant rats were
  separated into: vehicle group (VEH); and MG group, MG (60 mg/kg), the treatment was
  carried out from PN3 to PN14. The litters were standardized 4 male and 4 female pups. At
  PN120, hearts were mounted in a Langendorff apparatus. 10’ of basal, 30’ ischemia and 30’
  of reperfusion were performed. Intraventricular systolic (ISP) and diastolic (IDP) pressure,
  dP/dt + and - were recorded. AUC of each experiment was calculated, and the results were
  compared using two-way ANOVA with Sidak’s post-hoc test. Despite of no difference in the
  heart-to-body weight ratio, MG hearts presented reduced basal LV contractility in both male
  (p<0.01) and female (p<0.01) offspring. After ischemia, MG groups still presented decreased
  LV contractility in both male and female offspring (p<0.05). Glycotoxins exposure during the
  lactation leads to reduced intrinsic contractility at adulthood and reduced chances of surviving
  ischemic events.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68190
  Issue No: Vol. 27, No. ESPECIAL (2023)
HIGH-FAT DIET IN THE PERI-PUBERTAL PERIOD PROGRAMS CARDIOVASCULAR
DYSFUNCTION IN ADULT MALE WISTAR RATS
- Authors: Maiara Vanusa Guedes Ribeiro, Anna Rebeka F. Oliveira, Maria Natália C. Peres, Silvano Piovan, Lucas A. Ferreira, Rodrigo M. Gomes, Paulo Cezar F. Mathias, Kesia Gemima Palma Rigo Wutzow
  Abstract: Stress during peri-pubertal period can determine no communicable disease in adulthood. This
  study aims to evaluate whether high fat diet exposure during adolescence induces long-term
  cardiovascular dysfunctions. The ethic committee approved the animal protocol nº
  1527130815. Wistar rats, 30-days-old, were feed to a high fat diet (HFD, 35% lard w/w) until
  60-days-old, then fed a normal fat diet for a further 60 days dietary recovery. Control animals
  ate normal fat diet throughout life. Body weight and food intake were evaluated weekly. At
  PN120, cardiovascular and histological analysis were done. Student´s T test were applied.
  PN120, feed by HFD during adolescence increase systolic and diastolic blood pressure (upper
  10% and 4%, respectively; p <0.05 and p <0.005). Using ganglionic blocker, hexamethonium
  (30 mg/kg, iv), blood pressure was greater in the HFD rats (26%; p <0,01). Increased
  perivascular and interstitial fibrosis (42% and 62% respectively; p<0,01 and p<0,001) were
  observed in the HFD rats, accompanied by a left ventricular hypertrophy (29%; p<0,001).
  Rats who were fed with HFD during peri-pubertal age present hypertension, hyperactivity of
  the vascular sympathetic nervous system and morphological cardiac alterations in adulthood.
  Current study remarks that adolescence is a plastic phase which permit to programs diseases
  later in life.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68191
  Issue No: Vol. 27, No. ESPECIAL (2023)
HIGH-CALORIE DIET DURING LACTATION PROGRAMS EARLY HYPERPHAGIA IN MALE AND
FEMALE NEWBORN RATS
- Authors: Aline Milena D. Rodrigues, Manoela F. Antunes, Luís P. H. Rodrigues da Silva, Júlio Cezar de Oliveira
  Abstract: Obesity is a serious and concerning disease for public health. Breastfeeding is crucial for
  metabolic programming and plays an important role in controlling newborn metabolism. The
  aim of this study was to analyze weight gain and milk consumption in male and female
  newborn rats whose mothers were fed a high-calorie diet during the breastfeeding period. At
  delivery, female Wistar rats were divided into two groups, one was fed a hypercaloric diet
  (4,500 kcal/g and sucrose solution at 10%; Ob group), while control dams were fed
  commertial diet (Cont group). Pups were weighed every two days, and milk intake was
  recorded on the 6 th , 11 th and 16 th days old. At 22 days old, the pups were weaned, and the
  mother rats were euthanized for analysis. At 6 th day olds, Ob males consumed 42.7% more
  milk than Cont, while Ob females consumed 33.9% more than Cont (P<0.001). On the 11 th
  day of life, Ob males consumed 42.7% more than Cont, while Ob females consumed 33.9%
  more than Cont (P<0.05). On the 16 th day, Ob males consumed 39.3% more than Cont
  (P<0.001), while Ob females consumed 29.8% more than Cont (P<0.01). During the
  breastfeeding period, there was an increase in body mass of Ob males by 21.7% (P<0.05) and
  in females by 10%. It was observed that Ob males showed a weight increase of 18.65%
  compared to Ob females (P<0.001), while among the Cont group pups, no significant
  difference was observed between males and females. We concluded that offspring breastfed
  by mothers who consumed a high-calorie diet during lactation are more likely to develop
  early hyperphagia, and a greater risk of developing obesity.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68194
  Issue No: Vol. 27, No. ESPECIAL (2023)
METABOLIC DYSFUNCTIONS OF PERIPUBERTAL PROTEIN RESTRICTION IN MALE RATS
- Authors: Gabriel Kian Guimarães Lopes, Anna R. O. Ferreira, Maiara V. G. Ribeiro, Maria N. C. Peres, Leticia F. Barbosa, Scarlett R. Raposo, Mariana D. Fernandes, Marcos V. Martins, Douglas L. Almeida, Paulo Cezar de Freitas Mathias
  Abstract: The current study investigate sound effects of low-protein diet (LP) during peripubertal period
  to induce metabolic dysfunctions. In our study (CEUA 2910011021), 30 days-old Wistar male
  rats received LP (4% protein) and control rats were fed a 20.5% protein diet, for 60 days (NP
  group). Biometric parameters was taken and glucose, total and HDL-cholesterol, and
  triglycerides were evaluated from plasma samples. Glycemia homeostasis was evaluated by
  OGTT and ITT, while hepatic parameters, including quantitative analysis of fat, triglycerides
  and cholesterol in the liver was evaluated ex vivo. At 60 days, LP rats showed inferior body
  mass (P=0.003), food intake (P=0.05), and nose-tail length (P=0.0001), compared to NP.
  Regarding biochemical assays, LP rats showed higher serum glucose (P=0.008); however,
  triglyceride (P=0.002), total cholesterol (P=0.02), and HDL levels (P<0.0001) were lower in
  LP animals compared to NP group. LP rats also had minor glucose tolerance (P=0.02) and
  greater insulin sensitivity (P=0.01). In the hepatic parameters, LP rats showed an increase in
  liver fat (P<0.0001), hepatic triglycerides (P<0.0001), cholesterol (P<0.0001), and liver
  weight (P<0.0001). Considering the present data, peripuberty protein restriction induced
  metabolic and hepatic dysfunctions at the end of puberty. Therefore, malnourished adolescent
  animals are at higher risk of developing metabolic syndrome in adulthood.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68195
  Issue No: Vol. 27, No. ESPECIAL (2023)
EXPOSURE OF YOUNG ANIMALS TO NUTRITIVE AND NON- NUTRITIVE SWEETENERS AND
THEIR METABOLIC EFFECTS IN ADULTHOOD
- Authors: Luana Constantinov Jamarino, Letícia Ferreira Barbosa, Carolina Santos Campos de Oliveira, Eduardo de Oliveira Santos, Cecília Edna Mareze-Costa
  Abstract: Sucralose (SCL) is one of the most widely used edulcorants in the world, including by
  children and adolescents, but its effects on metabolic programming are not sufficient and
  conclusive. In this study we evaluated whether ingestion of SCL or sucrose (SUC) by young
  rats interferes with metabolic parameters in the adult rat. We used 21-day-old male wistar
  rats, which received pure water (control) or water added SUC or SCL, until 60 days of age.
  When they reached 90 days of age, half of the animals were fed a high fat diet (HFD). The
  animals were euthanized at 120 days of age. When fed only the standard diet, SUC intake in
  the young phase caused significant (p<0,05; ANOVA) increase in body weight (11%),
  adiposity (48%), insulinemia (53%) and insulin resistance (56%) and reduced lipolytic
  response to adrenergic stimulation (53%) in the adult animal. When fed the HFD diet, both
  sweeteners causing significant (p<0,05; ANOVA) increases in adiposity (SUC=32%;
  SCL=36%) and liver fat (SUC=34%; SCL=41%), with increased insulinemia (75%) only in
  the SLC group. We conclude that adding SUC and SCL to the diet during youth may increase
  the risks of obesity, insulin resistance and hepatic steatosis in adulthood and accentuate the
  metabolic disturbances caused by HFD diets.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68198
  Issue No: Vol. 27, No. ESPECIAL (2023)
NICOTINE EXPOSURE DURING BREASTFEEDING ALTERS CARDIAC PARAMETERS IN MALE
AND FEMALE OFFSPRING
- Authors: Carla Carolina Rodrigues da Silveira, Marcos Divino Ferreira-Junior, Keilah Valéria Naves Cavalcante, Ariel Penha Carvalho da Mota, Patrícia Cristina Lisbôa da Silva, Rodrigo Mello Gomes
  Abstract: Nicotine is one of the main psychoactive and toxic substances found in tobacco and its
  exposure during the postnatal period can cause several damages to the health of the child
  during intrauterine development and in adult life. The aim of this study was to evaluate the
  effect of maternal postnatal nicotine exposure on cardiac parameters in adult rat offspring.
  Weight monitoring, echocardiography and organ collections were performed. In conclusion,
  offspring from mothers exposed to nicotine develops cardiac hypertrophy, further male
  offspring were more affected.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68201
  Issue No: Vol. 27, No. ESPECIAL (2023)
MATERNAL FOOD RESTRICTION DURING LACTATION PROMOTES RENAL DYSFUNCTION IN
THE MALE OFFSPRING AT ADULTHOOD
- Authors: Maria Eduarda R. da Silva, Keilah V. N. Cavalcante, Marcos Divino Ferreira-Junior, Ariel Penha Carvalho da Mota, Isadora Silva Rosa, Jose Antonio Barbosa Lima Sobrinho, Rodrigo Mello Gomes
  Abstract: The aim of this work is to evaluate the renal function and the morphology of the kidneys in
  the offspring, of both sexes, of mothers who underwent a 50% food restriction during
  lactation. Pregnant Wistar rats were divided into two experimental groups: Control group
  (CO) fed with standard diet and Food restriction (FR) fed with standard diet restricted to 50%
  of the daily food intake of CO mothers. The intervention was carried out from the 1st to the
  14th day of lactation, after from the 14th to the 21st day of lactation. The offspring of both
  groups were fed with standard diet and water ad libitum until 120-day-old. At the end of the
  experimental period 24-hour urine collection was performed. After euthanasia biochemical
  and histological analysis were performed. Male and female FR offspring showed no change in
  body weight or kidney weight compared to control counterparts. Male FR showed a reduction
  in the glomerular filtration rate (CO 3.22±0.35 vs FR 2.25±0.12; ml/min) and increased total
  urinary protein (CO 82.82±11.79 vs FR 123.8±8.37; mg/dL) compared to the CO offspring.
  We observed a reduction in glomerular area in the male FR (CO 7259±271.7 vs FR
  6349±106.3; μm 2 ) in comparison to CO offspring. In conclusion, maternal food restriction
  causes functional and morphological kidney damage in adult males, being a sex-specific
  response since females did not show alterations.
  PubDate: 2023-05-10
  DOI: 10.4025/arqmudi.v27iESPECIAL.68202
  Issue No: Vol. 27, No. ESPECIAL (2023)
ASSOCIATION OF POLYMORPHISM IN TICAM1 GENE WITH PROTECTION TO
MODERATE/SEVERE COVID-19
- Authors: Matheus Braga, Pedro E. Santiago Silva, Aléia H. U. Yamanaka, Victor H. Souza, Sergio Grava, Ana F. Sela, Janisleya S. F. Neves, Joana Zacarias, Jeane Jeane Eliete Laguila Visentainer
  Abstract: Clinical outcomes of Covid-19 have shown variability between individuals and populations.
  TICAM1 gene encodes the TRIFF protein, essential in the antiviral response, then the purpose
  of the study was to assess the effect of the polymorphism (rs2292151) G>A of TICAM1 gene
  on the severity of COVID-19 in individuals from the northern region of Paraná. A case-control
  study was performed, including patients, 100 mild and 50 moderate/severe Covid-19 cases,
  classified according to the WHO, by Hospital Paraná, Maringá, Brazil. The exclusion criteria
  used were: patients with heart disease, liver disease, other respiratory diseases, HIV, and cancer.
  The (rs2292151) G>A polymorphism was genotyped by qPCR and statistical analysis was
  performed using logistic regression in the SNPStats software. The genotype distribution was
  according to the expected in the Hardy-Weinberg equilibrium. There was an association
  between the A/A genotype in a codominant model with protection against the severity of the
  disease (OR=0.14, 95% CI 0.03.-0.75, P = 0.01). The frequency of genotype A/A was 12% in
  mild cases and 4% in serious cases; the G/A was 44% in mild cases and 30% in serious cases,
  and the G/G was 44% in mild cases and 66% in serious cases. We can conclude that the A/A
  genotype (in a codominant genetic model) of the polymorphism (rs2292151) G>A was
  associated with a protection factor for moderate/severe Covid-19 in this population, however,
  the genotype determination should be done in a high number of patients.
  PubDate: 2023-05-09
  DOI: 10.4025/arqmudi.v27iESPECIAL.68168
  Issue No: Vol. 27, No. ESPECIAL (2023)
IMPACTS OF PHYSICAL EXERCISE ON POST-COVID-19 SEQUELAE IN PATIENTS TREATED
AT ACADEMY CLINIC SCHOOL OF PHYSICAL EDUCATION
- Authors: Felipe Muniz Rodrigues, Diego Bessa Dantas, Vinicius Muller Reis Weber, Vinicius Muller Reis Weber, Marcos Roberto Queiroga
  Abstract: The COVID-19 pandemic has had a significant impact on global health, with patients
  experiencing post-infection sequelae such as dyspnea, chronic fatigue, body aches and
  memory loss. This study aims to investigate the effectiveness of a physical exercise program
  in improving these symptoms. The database analyzed was from the Clinica e Academia
  school of physical education at the State University of the Midwest, which receives patients
  recovering from these symptoms. A structured anamnesis was performed, in which patients
  reported pre- and post-COVID-19 conditions. Four variables were investigated: dyspnea,
  chronic fatigue, body aches and memory loss. Training consisted of resistance and aerobic
  exercises, twice a week, monitored by heart rate and subjective perception of exertion, with
  workload progressing whenever possible. Each patient received a prescription based on the
  American College for Prescription of Exercise for Health guidelines. There was a decrease in
  chronic fatigue (57.1% to 28.6%), dyspnea (50% to 21.4%), body aches (35.7% to 7.1%) and
  memory loss (35.7% to 28.6%). These findings suggest that the implementation of physical
  exercises can attenuate post-covid19 symptoms. However, this descriptive study is not
  conclusive and well-designed essays that can better elucidate the subject are recommended.
  PubDate: 2023-05-09
  DOI: 10.4025/arqmudi.v27iESPECIAL.68172
  Issue No: Vol. 27, No. ESPECIAL (2023)
COVID-19 DEATHS AMONG THE ELDERLY IN A MUNICIPALITY IN NORTHWESTERN
PARANÁ, BETWEEN THE YEARS 2020 AND 2021
- Authors: Natália Quevedo dos Santos, Karine Franciele Toldo de Toledo, Sônia Maria Marque Gomes Bertolini
  Abstract: In December 2019, a new virus called SARS-CoV-2 was identified. The World Health
  Organization (WHO) named the disease caused by the new virus COVID-19 (coronavirus
  disease, year 2019), and in March 2020, it was declared a pandemic. Since then, there has
  been a global effort to produce information in order to unravel the clinical, epidemiological,
  and prognostic factors of the disease. By identifying the main risk factors, it is possible to
  carry out early monitoring of the most vulnerable groups, reducing the chance of unfavorable
  clinical outcomes. It is a consensus that advanced age is associated with an increase in
  morbidity and mortality. This study aimed to verify the occurrence of COVID-19 deaths
  among the elderly in the city of Maringá, Paraná, in the years 2020 and 2021, as well as their
  association with gender. This research is characterized as a retrospective quantitative study.
  The data were obtained from the Notifiable Diseases Information System (Sinan). The
  dependent variable was the occurrence of death in elderly patients, and the independent
  variables were gender and year of occurrence. Descriptive and inferential statistics were used
  to analyze the data. Results showed that in 2020, there were 514 deaths (40.5% female and
  59.5% male). In 2021, the number of deaths increased exponentially, reaching 2025 cases
  (39.6% female and 60.4% male). The highest occurrence of COVID-19 deaths among the
  elderly occurred in 2021 and in males. Based on this profile, specific strategies and care lines
  should be studied for this more vulnerable population in times of pandemics.
  PubDate: 2023-05-09
  DOI: 10.4025/arqmudi.v27iESPECIAL.68173
  Issue No: Vol. 27, No. ESPECIAL (2023)
EVALUATION OF CYTOKINE IL-6 PROFILE IN PATIENTS HOSPITALIZED WITH COVID-19
IN THE 15TH REGIONAL HEALTH OF PARANA
- Authors: Brenda Barroso Pelegrini, Fernanda Ferreira Evangelista, Felipe Aparecido Lacerda de Andrade, Letícia Nishi, Cristiane Maria Colli, Willian Costa Ferreira, Priscilla de Laet Santana
  Abstract: COVID-19 is a viral infection caused by SARS-CoV-2 coronavirus, discovered in Wuhan,
  China, in 2019. It is a potentially serious disease with high transmissibility, characterized by
  worsening due to the exacerbated immune response, coordinated from pro-inflammatory
  cytokines signaling, leading to tissue damage. Objective: to evaluate the immunological
  profile of patients affected by COVID-19 through protein measurement that modulates
  immune response and has a great influence on cytokine storms. Materials and methods: the
  serum concentration analysis of the cytokine IL-6 was performed in patients with the disease.
  This procedure was developed through the duplicate capture ELISA technique, following
  manufacturer's standards, with the serum used from University Hospital of Maringa (HUM)
  patients who were hospitalized in the ward (G2), and who were in the intensive care unit but
  died (G3), as well as negative samples for COVID-19 (G1-control) from blood donors stored
  in the Maringa Regional Blood Center. Data were analyzed using GraphPad Prism software,
  version 7.0. Results: an increase in IL-6 was observed in more severe patients (G3).
  Conclusion: the cytokine IL-6 has been shown to be important prognostic markers of
  worsening of COVID-19.
  PubDate: 2023-05-09
  DOI: 10.4025/arqmudi.v27iESPECIAL.68174
  Issue No: Vol. 27, No. ESPECIAL (2023)