Subjects -> BIOLOGY (Total: 3134 journals)
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BIOLOGY (1491 journals)                  1 2 3 4 5 6 7 8 | Last

Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 27)
Abasyn Journal of Life Sciences     Open Access   (Followers: 4)
ACS Pharmacology & Translational Science     Hybrid Journal   (Followers: 3)
ACS Synthetic Biology     Hybrid Journal   (Followers: 38)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 5)
Acta Biologica Marisiensis     Open Access   (Followers: 3)
Acta Biologica Sibirica     Open Access   (Followers: 2)
Acta Biologica Turcica     Open Access   (Followers: 2)
Acta Biomaterialia     Hybrid Journal   (Followers: 32)
Acta Biotheoretica     Hybrid Journal   (Followers: 3)
Acta Chiropterologica     Full-text available via subscription   (Followers: 5)
acta ethologica     Hybrid Journal   (Followers: 7)
Acta Fytotechnica et Zootechnica     Open Access   (Followers: 3)
Acta Ichthyologica et Piscatoria     Open Access   (Followers: 5)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales     Open Access  
Acta Neurobiologiae Experimentalis     Open Access  
Acta Scientiae Biological Research     Open Access   (Followers: 1)
Acta Scientiarum. Biological Sciences     Open Access   (Followers: 2)
Acta Scientifica Naturalis     Open Access   (Followers: 4)
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis     Open Access   (Followers: 2)
Acta Universitatis Lodziensis : Folia Biologica et Oecologica     Open Access  
Actualidades Biológicas     Open Access   (Followers: 1)
Advanced Biology     Hybrid Journal   (Followers: 1)
Advanced Health Care Technologies     Open Access   (Followers: 12)
Advanced Journal of Graduate Research     Open Access   (Followers: 2)
Advanced Membranes     Open Access   (Followers: 7)
Advanced Quantum Technologies     Hybrid Journal   (Followers: 5)
Advances in Bioinformatics     Open Access   (Followers: 24)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4)
Advances in Biology     Open Access   (Followers: 14)
Advances in Biomarker Sciences and Technology     Open Access   (Followers: 2)
Advances in Biosensors and Bioelectronics     Open Access   (Followers: 8)
Advances in Cell Biology/ Medical Journal of Cell Biology     Open Access   (Followers: 26)
Advances in Ecological Research     Full-text available via subscription   (Followers: 46)
Advances in Environmental Sciences - International Journal of the Bioflux Society     Open Access   (Followers: 17)
Advances in Enzyme Research     Open Access   (Followers: 10)
Advances in High Energy Physics     Open Access   (Followers: 26)
Advances in Human Biology     Open Access   (Followers: 9)
Advances in Life Science and Technology     Open Access   (Followers: 14)
Advances in Life Sciences     Open Access   (Followers: 6)
Advances in Marine Biology     Full-text available via subscription   (Followers: 29)
Advances in Tropical Biodiversity and Environmental Sciences     Open Access   (Followers: 5)
Advances in Virus Research     Full-text available via subscription   (Followers: 8)
Adversity and Resilience Science : Journal of Research and Practice     Hybrid Journal   (Followers: 3)
African Journal of Ecology     Hybrid Journal   (Followers: 18)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 12)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 3)
Ageing Research Reviews     Hybrid Journal   (Followers: 12)
Aggregate     Open Access   (Followers: 3)
Aging Cell     Open Access   (Followers: 23)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
AJP Cell Physiology     Hybrid Journal   (Followers: 13)
AJP Endocrinology and Metabolism     Hybrid Journal   (Followers: 14)
AJP Lung Cellular and Molecular Physiology     Hybrid Journal   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
Alces : A Journal Devoted to the Biology and Management of Moose     Open Access  
Alfarama Journal of Basic & Applied Sciences     Open Access   (Followers: 15)
All Life     Open Access   (Followers: 6)
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 7)
American Journal of Bioethics     Hybrid Journal   (Followers: 17)
American Journal of Human Biology     Hybrid Journal   (Followers: 18)
American Journal of Medical and Biological Research     Open Access   (Followers: 5)
American Journal of Plant Sciences     Open Access   (Followers: 24)
American Journal of Primatology     Hybrid Journal   (Followers: 17)
American Naturalist     Full-text available via subscription   (Followers: 80)
Amphibia-Reptilia     Hybrid Journal   (Followers: 5)
Anaerobe     Hybrid Journal   (Followers: 3)
Analytical Methods     Hybrid Journal   (Followers: 7)
Analytical Science Advances     Open Access   (Followers: 1)
Anatomia     Open Access   (Followers: 14)
Anatomical Science International     Hybrid Journal   (Followers: 3)
Animal Cells and Systems     Hybrid Journal   (Followers: 4)
Animal Microbiome     Open Access   (Followers: 6)
Animal Models and Experimental Medicine     Open Access  
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 2)
Annales Henri Poincaré     Hybrid Journal   (Followers: 2)
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia     Open Access   (Followers: 1)
Annals of Applied Biology     Hybrid Journal   (Followers: 7)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Human Biology     Hybrid Journal   (Followers: 6)
Annals of Science and Technology     Open Access   (Followers: 2)
Annual Research & Review in Biology     Open Access   (Followers: 1)
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 18)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 23)
Annual Review of Cancer Biology     Full-text available via subscription   (Followers: 3)
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 40)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 31)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 10)
Anthropological Review     Open Access   (Followers: 28)
Antibiotics     Open Access   (Followers: 12)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 7)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 3)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 5)
Applied Biology     Open Access  
Applied Bionics and Biomechanics     Open Access   (Followers: 4)
Applied Phycology     Open Access   (Followers: 1)
Applied Vegetation Science     Full-text available via subscription   (Followers: 9)
Aquaculture Environment Interactions     Open Access   (Followers: 7)
Aquaculture International     Hybrid Journal   (Followers: 25)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 9)
Aquatic Biology     Open Access   (Followers: 9)
Aquatic Ecology     Hybrid Journal   (Followers: 42)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 16)
Aquatic Science and Technology     Open Access   (Followers: 4)
Aquatic Toxicology     Hybrid Journal   (Followers: 26)
Arabian Journal of Scientific Research / المجلة العربية للبحث العلمي     Open Access  
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 1)
Archives of Biological Sciences     Open Access  
Archives of Microbiology     Hybrid Journal   (Followers: 9)
Archives of Natural History     Hybrid Journal   (Followers: 8)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 6)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arctic     Open Access   (Followers: 9)
Arid Ecosystems     Hybrid Journal   (Followers: 2)
Arquivos do Instituto Biológico     Open Access   (Followers: 1)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 1)
Arthropod Systematics & Phylogeny     Open Access   (Followers: 5)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 2)
Artificial Intelligence in the Life Sciences     Open Access   (Followers: 1)
Asian Bioethics Review     Full-text available via subscription   (Followers: 2)
Asian Journal of Biological Sciences     Open Access   (Followers: 2)
Asian Journal of Biology     Open Access  
Asian Journal of Biotechnology and Bioresource Technology     Open Access  
Asian Journal of Cell Biology     Open Access   (Followers: 4)
Asian Journal of Developmental Biology     Open Access   (Followers: 1)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 3)
Asian Journal of Nematology     Open Access   (Followers: 4)
Asian Journal of Poultry Science     Open Access   (Followers: 3)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 8)
Autophagy     Hybrid Journal   (Followers: 8)
Avian Biology Research     Hybrid Journal   (Followers: 4)
Avian Conservation and Ecology     Open Access   (Followers: 18)
Bacterial Empire     Open Access   (Followers: 1)
Bacteriology Journal     Open Access   (Followers: 2)
Bacteriophage     Full-text available via subscription   (Followers: 2)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access  
Berita Biologi     Open Access  
Between the Species     Open Access   (Followers: 2)
BIO Web of Conferences     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bio-Lectura     Open Access  
BIO-SITE : Biologi dan Sains Terapan     Open Access  
Bioactive Compounds in Health and Disease     Open Access   (Followers: 1)
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 4)
BioCentury Innovations     Full-text available via subscription   (Followers: 2)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 18)
Biochimie     Hybrid Journal   (Followers: 2)
BioControl     Hybrid Journal   (Followers: 2)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal   (Followers: 1)
BIODIK : Jurnal Ilmiah Pendidikan Biologi     Open Access  
BioDiscovery     Open Access   (Followers: 2)
Biodiversitas : Journal of Biological Diversity     Open Access   (Followers: 2)
Biodiversity : Research and Conservation     Open Access   (Followers: 30)
Biodiversity Data Journal     Open Access   (Followers: 7)
Biodiversity Informatics     Open Access   (Followers: 3)
Biodiversity Information Science and Standards     Open Access   (Followers: 2)
Biodiversity Observations     Open Access   (Followers: 2)
Bioeduca : Journal of Biology Education     Open Access   (Followers: 2)
Bioeduscience     Open Access   (Followers: 3)
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 1)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 3)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
Bioethica     Open Access   (Followers: 1)
Bioethics     Hybrid Journal   (Followers: 20)
BioéthiqueOnline     Open Access   (Followers: 1)
Biogeographia : The Journal of Integrative Biogeography     Open Access   (Followers: 2)
Biogeosciences (BG)     Open Access   (Followers: 18)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 3)
Bioinformatics     Hybrid Journal   (Followers: 288)
Bioinformatics Advances : Journal of the International Society for Computational Biology     Open Access   (Followers: 4)
Bioinformatics and Biology Insights     Open Access   (Followers: 14)
Biointerphases     Open Access   (Followers: 1)
Biojournal of Science and Technology     Open Access  
BioLink : Jurnal Biologi Lingkungan, Industri, Kesehatan     Open Access  
Biologia     Hybrid Journal   (Followers: 1)
Biologia Futura     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 6)
Biological Control     Hybrid Journal   (Followers: 6)

        1 2 3 4 5 6 7 8 | Last

Similar Journals
Journal Cover
Al-Kauniyah : Jurnal Biologi
Number of Followers: 0  

  This is an Open Access Journal Open Access journal
ISSN (Print) 1978-3736 - ISSN (Online) 2502-6720
Published by UIN Syarif Hidayatullah Jakarta Homepage  [9 journals]
  • Malay Apple (Syzygium malaccense) Fruit Calendar: A Case in Central Java

    • Authors: Pudji Widodo, Elly Proklamasiningsih
      Pages: 279 - 286
      Abstract:  AbstractMalay apple (Syzygium malaccense) is a kind of popular fruit tree originally from Indo-Malayan-Region or South East Asia. There are many environmental factors affecting the flowering time, infrutescence number, fruit size, and fruit number, such as fruit position on the spur, spur size, fruit number set on the spur, and the date of flowering. The objectives of this study were to find out the fruit production during dry and wet seasons, so that fruit calendar can be determined.  The method used in this study was survey and observation on Malay apple trees in Banyumas, Purbalingga, and Kebumen from 2018 to 2021. The results of this study showed that June, July, and August when the precipitation was the lowest, the fruit number is the highest, and fruit size is the smallest (145–175 mL). On the other hand, when the precipitation was high (September-April), the fruit was the largest (180–500 mL).  Fruit harvest time might change every year depending on climatic factors such as average daily temperature and precipitation. There is no specific harvest time for Malay apples. The implementation of the results of this research is that we do not need to predict when the Malay apple harvest season will be, because the harvest time can be at any time.AbstrakJambu bol (Syzygium malaccense) merupakan salah satu jenis pohon buah-buahan populer yang berasal dari Wilayah Indo-Malayan atau Asia Tenggara. Banyak faktor lingkungan yang memengaruhi waktu berbunga, jumlah bunga, ukuran buah, dan jumlah buah, seperti posisi buah pada tajuk, ukuran tajuk, jumlah buah tiap dompol, dan tanggal berbunga. Penelitian ini bertujuan untuk mengetahui produksi buah pada musim kemarau dan musim hujan, sehingga dapat ditentukan kalender buah. Metode yang digunakan dalam penelitian ini adalah survei dan observasi pada pohon jambu bol di Banyumas, Purbalingga, dan Kebumen dari tahun 2018 sampai dengan tahun 2021. Hasil penelitian ini menunjukkan bahwa pada bulan Juni, Juli, dan Agustus curah hujan paling rendah, jumlah buahnya paling tinggi, dan ukuran atau volume buahnya relatif  paling kecil (145–175 mL). Sebaliknya, ketika curah hujan rata-rata tinggi (September-April), buahnya berukuran besar, dengan volume 180–500 mL. Waktu panen buah dapat berubah setiap tahun tergantung pada faktor iklim seperti suhu harian rata-rata dan curah hujan. Hasil penelitian ini menunjukkan bahwa tidak ada waktu yang spesifik untuk waktu panen jambu bol. Implementasi dari hasil penelitian ini adalah bahwa kita tidak perlu memprediksi kapan musim panen jambu bol, karena waktu panen bisa kapan saja.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.23872
      Issue No: Vol. 16, No. 2 (2023)
       
  • Pengelolaan dan Pengayaan Koleksi Biji Begonia di Bank Biji Kebun Raya
           Cibodas Jawa Barat

    • Authors: Intani Quarta Lailaty, Yulita Ratna Mufida, Vandra Kurniawan, Muhammad Efendi
      Pages: 287 - 300
      Abstract: AbstrakBank biji merupakan salah satu solusi yang baik dalam pemeliharaan plasma nutfah secara ex-situ, karena dianggap lebih efektif dan efisien dalam penyimpanan. Bank biji di Kebun Raya Cibodas (KRC) telah mengoleksi 147 jenis biji dari 102 marga dan 61 suku. Penyimpanan biji ukuran kecil masih menjadi suatu tantangan dan peluang tersendiri untuk diteliti dan dikembangkan. Dalam tulisan ini akan dibahas mengenai pengelolaan dan pengayaan koleksi biji ukuran kecil, seperti Begonia, di bank biji KRC. Prosedur pengelolaan biji di bank biji KRC meliputi pemanenan, pemrosesan, pengujian, dan penyimpanan biji. Tiap jenis biji akan diberi nomor koleksi sesuai prosedur di bank biji. Pengujian viabilitas biji menggunakan metode cut test dan uji Triphenyl Tetrazolium Chloride (TTC). Biji-biji yang dikoleksi di bank biji KRC berasal dari tumbuhan koleksi kebun dan hasil eksplorasi biji secara khusus di wilayah Jawa dan Sumatra. Terdapat 12 penambahan jenis biji Begonia di bank biji KRC, antara lain Begonia multangula, B. muricata, B. isoptera, B. areolata, B. stictopoda, B. repanda, B. chiasmogyna, dan B. atricha yang berasal dari Gunung Tilu, Jawa Barat dan Gunung Slamet, Jawa Tengah. Uji viabilitas menggunakan TTC menunjukkan warna merah dengan perlakuan perendaman 24 jam dan pre-treatment pada B. repanda. B. longifolia memiliki karakter simpan serupa biji ortodoks berdasarkan studi literatur dan 100 seed test termodifikasi.AbstractSeed bank is a good solution for ex-situ germplasm maintenance. It is considered more effective and efficient in storage. The seed bank at Cibodas Botanic Gardens (CBG) has collected 128 types of seeds from 102 genera and 61 families. Storage of microscopic seeds still be a challenge and opportunity to be observered and developed. In this paper, we will discuss the management and enrichment of microscopic seed collections, such as Begonia, at the CBG’s seed bank. Seed management procedures at the CBG’s seed bank include harvesting, processing, testing and storage of seeds. Each type of seed will be assigned a collection number according to the procedure at the seed bank. The seed viability test used the cut test method and the Triphenyl Tetrazolium Chloride (TTC) test. The seeds collected from collection plants of CBG and seed exploration specifically in Java and Sumatra. There are 12 additional species of Begonia seeds in the CBG’s seed bank, including Begonia multangula, B. muricata, B. isoptera, B. areolata, B. stictopoda, B. repanda, B. chiasmogyna, and B. atricha from Mount Tilu, West Java and Mount Slamet, Central Java. Viability test using TTC showed a red color with 24 hours immersion treatment and pre-treatment on B. repanda. B. longifolia has storage characteristics similar to orthodox seeds based on literature studies and 100 modified seed tests.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.24265
      Issue No: Vol. 16, No. 2 (2023)
       
  • Tinjauan Biosorpsi Logam Berat Pb dan Cd Oleh Jamur Makro

    • Authors: Noverita Noverita, Nuniek Ina Ratnaningtyas, Endang Sukara, Nuraeni Ekowati, Sri Lestari
      Pages: 309 - 326
      Abstract: AbstrakKeberadaan logam berat perlu ditanggulangi, salah satunya dengan cara biosorpsi. Biosorpsi merupakan salah satu metode remediasi yang paling tepat digunakan dalam menanggulangi pencemaran logam berat. Proses secara efisien dapat menyerap logam berat terlarut bahkan logam berat yang ada dalam larutan kompleks yang sangat encer. Jamur makro merupakan contoh biosorben yang dapat digunakan dalam biosorpsi. Pemanfaatan jamur makro sebagai biosorben sangat tepat dilakukan karena keanekaragaman jenisnya tinggi, cepat tumbuh dengan kemampuan metabolisme yang beragam pada berbagai senyawa organik dan anorganik, mudah didapatkan, lebih aman karena tidak menyebabkan korosi atau menghasilkan bahan berbahaya, teknologinya sederhana, perawatannya mudah dan produk akhir dapat didaur ulang, serta dapat mengakumulasi logam berat dengan kosentrasi tinggi. Jenis-jenis jamur makro yang sudah digunakan dalam biosorpsi logam berat di beberapa negara antara lain Agaricus bisporus, Auricularia polytricha, Calocybe indica, Ganoderma carnosum, Flammulina velutipes, Fomes fasciatus, dan Volvariella volvacea. Sementara itu, penelitian penggunakan jamur makro di Indonesia dalam biosorpsi logam berat sangat terbatas yakni pada jenis Phanerochaete chrysosporium, Omphalina sp., dan Pholiota sp. Mengingat tingginya keanekaragaman jamur makro di Indonesia, maka potensi biosorpsi logam berat khususnya Pb dan Cd oleh jamur makro sangat berpeluang untuk diteleti lebih lanjut.AbstractHeavy metals can accumulate in seawater, sediments and in the marine biota that live in them, eventually entering the food chain which is very dangerous to health. The presence of these heavy metals needs to be overcome, one of which is by means of biosorption. Biosorption is one of the most appropriate remediation methods used in tackling heavy metal pollution. The biosorption process can efficiently absorb dissolved heavy metals and even heavy metals present in very dilute complex solutions. Macro fungi are examples of biosorbents that can be used in biosorption. Utilization of macro fungi as biosorbents is very appropriate because of the high diversity of species, fast growing with diverse metabolic abilities on various organic and inorganic compounds, easy to obtain, strong morphology, safer because they do not cause corrosion or produce harmful materials, simple technology, easy maintenance and the final product can be recycled, can accumulate heavy metals with high concentrations. The types of macro fungi that have been used in the biosorption of heavy metals in several countries include Agaricus bisporus, Auricularia polytricha, Calocybe indica, Ganoderma carnosum, Flammulina velutipes, Fomes fasciatus, and Volvarella volvacea. Meanwhile in Indonesia, research on the use of macro fungi in heavy metal biosorption is very limited to the Phanerochaete chrysosporium, Omphalina sp. and Pholiota sp. Considering the high diversity of macro fungi in Indonesia, the potential for biosorption of heavy metals, especially Pb and Cd by macro fungi, is very likely to be investigated further.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.24569
      Issue No: Vol. 16, No. 2 (2023)
       
  • Potensi Isolat Bakteri Selulolitik Toleran Panas Asal Tanah, Sampah Dapur,
           dan Kotoran Sapi Dalam Biodegradasi Serasah Daun

    • Authors: Taruna Dwi Satwika, Hendro Pramono, Dwiana Muflihah Yulianti
      Pages: 327 - 335
      Abstract:  AbstrakBakteri selulolitik memainkan peranan penting dalam biodegradasi komponen selulosa pada sampah organik. Namun, proses pengomposan umumnya melewati fase termofilik (suhu mencapai 55 °C), sehingga tidak semua bakteri dapat bertahan. Sebanyak delapan isolat bakteri selulolitik telah berhasil diisolasi dari tanah, sampah dapur, dan kotoran sapi. Namun, isolat-isolat tersebut belum diketahui aktivitas selulolitiknya pada suhu tinggi dan kemampuannya dalam mendegradasi biomassa serasah daun. Tujuan penelitian ini adalah mengetahui aktivitas selulolitik isolat bakteri pada suhu tinggi secara kualitatif, mengetahui aktivitas selulolitik isolat bakteri secara kuantitatif, dan mengetahui kemampuan isolat bakteri selulolitik dalam mendegradasi biomassa serasah daun. Penelitian dilakukan dengan tahapan peremajaan isolat, skrining kualitatif aktivitas selulolitik isolat pada suhu ruangan, 45 °C dan 55 °C, skrining kuantitatif aktivitas selulolitik isolat, dan uji degradasi biomassa serasah daun. Sebanyak 6 dari 8 isolat bakteri menunjukkan aktivitas selulolitik pada medium Carboxy Methyl Celullose (CMC) Agar pada suhu 55 °C. Berdasarkan uji aktivitas enzim secara kuantitatif, 3 isolat (KS1, KS4, dan SD5) dengan aktivitas enzim tertinggi terpilih untuk pengujian degradasi serasah daun dan menunjukkan rata-rata aktivitas enzim secara berurutan 0,0074 UI/mL; 0,0080 UI/mL; 0,0159 UI/mL. Ketiga isolat mampu mempercepat proses degradasi serasah daun dan berpotensi sebagai agen pengomposan.AbstractHowever, the composting process generally passes through a thermophilic phase (55 °C), so that not all bacteria can survive. A total of 8 isolates of cellulolytic bacteria isolated from soil, kitchen waste, and cow dung have not yet known their cellulolytic activity at high temperatures and their ability to degrade leaf litter biomass. This study aimed to determine the cellulolytic activity of bacterial isolates at high temperatures qualitatively, to determine the cellulolytic activity of bacterial isolates quantitatively, and to determine the ability of these isolates to degrade leaf litter biomass. The research was carried out by reculture isolates; qualitative screening of isolate cellulolytic activity at room temperature, 45 °C and 55 °C; quantitative screening of isolate cellulolytic activity; and leaf litter biomass degradation test. Six of eight bacterial isolates showed cellulolytic activity on Carboxy Methyl Celullose (CMC) Agar medium at 55 °C. Three isolates (KS1, KS4, and SD5) with the highest enzyme activity were selected for the leaf litter degradation test and showed an average enzyme activity of 0.0074 UI/mL; 0.0080 UI/mL; 0.0159 UI/mL, respectively. The three isolates were able to accelerate the degradation process of leaf litter and have potential as composting agents.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.25616
      Issue No: Vol. 16, No. 2 (2023)
       
  • Produksi Antibodi Poliklonal Menggunakan Protein Rekombinan RBD-spike
           Untuk Deteksi SARS-CoV-2

    • Authors: Iryani Endah Febrianti, Yayuk Fatmawati, Intan Ria Neliana, Widhi Dyah Sawitri, Erlia Narulita, Bambang Sugiharto
      Pages: 336 - 346
      Abstract:  AbstrakSARS-CoV-2 merupakan virus yang menyebabkan Coronavirus Disease 2019 (COVID-19) di seluruh dunia dan sampai saat ini kasus terbaru masih terus dilaporkan. Diagnostic test merupakan hal yang krusial untuk dikembangkan. Prinsip diagnostic test COVID-19 berbasis antigen, yaitu mendeteksi virus SARS-CoV-2 melalui respon antibodi dari penderita. Penelitian ini bertujuan untuk memproduksi antibodi poliklonal menggunakan protein rekombinan RBD-Spike untuk mendeteksi virus SARS-CoV-2 berbasis antibodi. Penelitian dimulai dengan penentuan domain RBD-Spike menggunakan pensejajaran asam amino, dan konstruksi DNA untuk RBD-Spike pada vektor ekspresi pET28a menggunakan sintetik nukleotida. Produksi protein rekombinan RBD-Spike diekspresikan pada sel bakteri Escherichia coli. Purifikasi dilakukan untuk memperoleh protein RBD-Spike dan selanjutnya digunakan sebagai antigen untuk induksi antibodi poliklonal pada kelinci. Hasil penelitian menunjukkan bahwa ekspresi protein rekombinan RBD-Spike SARS-CoV-2 memerlukan induksi IPTG 0,1 mM dan terekspresi dalam bentuk inclusion bodies dengan ukuran 39 kDa. Purifikasi protein RBD-Spike dilakukan menggunaan resin afinitas NiNTA, elektroelusi, dan dialisis. Total protein RBD-Spike yang diperoleh sebanyak 4 mL dengan konsentrasi 10 mg/mL. Analisa Ouchterlony menunjukkan bahwa antibodi poliklonal terdeteksi pada minggu kedua setelah injeksi booster dan analisa spesifitas antibodi terhadap antigen menunjukkan bahwa antibodi poliklonal dapat mendeteksi protein RBD-Spike pada konsentrasi 0,1 µg. Selanjutnya diharapkan antibodi poliklonal dapat digunakan untuk deteksi keberadaan virus SARS-CoV-2 dan dapat dikembangkan untuk kit deteksi berbasis antibodi.AbstractSARS-CoV-2 is the virus that causes Coronavirus Disease 2019 (COVID-19) worldwide and the latest cases are still being reported until now. The diagnostic test is a crucial to be developed. The principle of the antigen-based COVID-19 diagnostic test is to detect the SARS-CoV-2 virus through antibody response from the patients. This study was conducted to produce polyclonal antibodies using recombinant protein RBD-Spike. The research was carried out by determining the RBD-Spike domain using amino acid alignment and constructing the DNA of RBD-Spike to the expression vector of pET28a using nucleotide synthesis. Production of RBD-Spike recombinant protein was expressed in Escherichia coli. Purification was carried out to obtain RBD-Spike protein and used to induce polyclonal antibody ina rabbit. The results showed that the expression of RBD-Spike recombinant protein required induction of IPTG 0.1 mM and was expressed in inclusion bodies with molecular size of 39 kDa. The purification of RBD-Spike protein was carried out using resin affinity, electroelution, and dialysis. The total protein of RBD-Spike obtained was 4 mL with a concentration of 10 mg/mL. Ouchterlony analysis revealed that polyclonal antibody was detected in the second week after booster injection and analysis of antibody specificity showed that polyclonal antibodies detected RBD-Spike protein at the concentration of 0.1µg of RBD-Spike protein. Moreover, it is expected that our polyclonal antibody detect the SARS-CoV-2 virus and can be developed for antibody-based detection kits.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.25664
      Issue No: Vol. 16, No. 2 (2023)
       
  • Rambutan Fruit Peel Extract Reduces Abnormal Sperm Morphology in Male
           Wistar Rats with Obesity

    • Authors: Iqlima Luthfiya, Oktania Sandra Puspita, Yudhi Nugraha, Fahri Fahrudin
      Pages: 347 - 355
      Abstract: AbstractObesity is an accumulation of excessive fat tissue in the body. Excessive fat tissue in the body lead to infertility by increased Reactive Oxygen Species (ROS) and decrease the hormone balance regulation, those things can be affected the process of spermatogenesis, especially sperm morphology. Rambutan peel was known as a source of antioxidants because it has phenolic compounds, so it has a protective effect on free radicals. The research purpose knew the effect of Rambutan Peel Extract (RPE) (Nephelium lappaceum) on abnormal sperm morphology of Wistar rats (Rattus novergicus) percentage induced with a High-Fat Diet (HFD). This study uses True experimental post control group design for this research on 30 male Wistar rats. Samples were divided into 5 groups. Group 1: induced HFD only, Group 2: only given RPE at a dosage of 30 mg/kilogram Body Weight (kg BW), Group 3, 4, and 5: induced HFD and RPE at dosage of 15 mg/kg BW, 30 mg/kg BW, and 60 mg/kg BW feed using sonde. The data were analysed using ANOVA One Way. The result showed that RPE has decreased the abnormal sperm morphology of male Wistar rats at dose of 15 mg/kg BW. This is the first study that observe the effect of RPE administration to sperm morphology of obese and non-obese group of rats, with larger rats’ population, several doses of the RPE extract, and longer time to complete one cycle of rat spermatogenesis.AbstrakObesitas diartikan sebagai akumulasi jaringan lemak berlebihan yang ada di dalam tubuh. Jaringan lemak yang berlebihan dapat menyebabkan ketidakseimbangan regulasi hormonal dan terbentuknya Reactive Oxygen Species (ROS). Kedua hal tersebut dapat mempengaruhi proses spermatogenesis sehingga dapat menyebabkan infertilitas, terutama pada morfologi sperma. Ekstrak Kulit Rambutan (EKR) diketahui memiliki efek sebagai antioksidan dikarenakan memiliki senyawa fenolik, senyawa tersebut dapat menangkal radikal bebas. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian EKR (Nephelium lappaceum) terhadap persentase morfologi abnormal sperma tikus Wistar (Rattus novergicus) yang diinduksi dengan Pakan Tinggi Lemak (PTL). Penelitian ini menggunakan desain True experimental post control group design pada 30 ekor tikus. Sampel dibagi menjadi 5 kelompok. Kelompok 1: hanya diberi PTL, Kelompok 2: hanya diberikan EKR dengan dosis 30 mg/kilogram Berat Badan (kgBB), Kelompok 3, 4 dan 5: diinduksi dengan PTL dan EKR dengan dosis 15 mg/kgBB, 30 mg/kgBB, dan 60 mg/kgBB. Pemberian PTL dan EKR dilakukan menggunakan sonde. Data dianalisis menggunakan uji ANOVA One Way. Hasil penelitian menunjukkan bahwa EKR dapat menurunkan jumlah morfologi abnormal pada kelompok dengan induksi PLT, dengan dosis yang paling efektif 15 mg/kgBB. Studi ini adalah yang pertama dalam mencari tahu efek pemberian RPE tehadap morfologi spermatozoa pada kelompok tikus obesitas dan tidak obesitas, dengan populasi tikus yang lebih besar, beberapa dosis ekstrak RPE, dan waktu yang lebih lama agar dapat menyelesaikan satu siklus spermatogenesis tikus.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.25729
      Issue No: Vol. 16, No. 2 (2023)
       
  • Varanus salvator As an Indicator of Turtle Nesting Season at Ngagelan
           Beach, Alas Purwo National Park (TNAP), Indonesia: A Conservatiom Effort

    • Authors: Siti Roudlotul Hikamah, Hariyanto Hariyanto, Nia Kurniawan
      Pages: 356 - 364
      Abstract: AbstractVarious turtle conservation efforts continue to be carried out, including protecting the eggs from predators. The study aimed to describe the behavior of turtles laying eggs using Varanus salvator as an indicator. The research was conducted at Ngagelan Beach, Alas Purwo National Park (TNAP), and around the Semi Natural Turtle Hatchery (PPSA), Banyuwangi, Indonesia. The research was conducted from January to December 2019. The type of research is quantitative and qualitative. The study results indicated that Varanus salvator visited this area only during the turtle nesting season. Lepidochelys olivacea dominates the types of turtles that land in TNAP. 1,056 L. Olivacea, 6 Chelonia mydas, Eretmochelys imbricata, and Dermochelys coriacea landed only one animal to lay eggs. Landing occurs from April to August, with peak landing in June. Varanus salvator was found roaming the area starting April, peaking in the second week of August and decreasing in October. Differences in landing time and arrival behavior of Varanus salvator are related to the incubation period of 40–50 days of turtle eggs. The smell of turtle eggs and dead hatchlings beckons V. salvator to this area. This behavior is because Varanus salvator has a strong sense of smell, detected by frequent tongue flicking.AbstrakBerbagai upaya konservasi penyu terus dilakukan, salah satunya melindungi telur dari pemangsa. Tujuan penelitian untuk mendeskripsikan perilaku penyu bertelur menggunakan Varanus salvator sebagai indikator serta upaya konservasii. Penelitian dilakukan di Pantai Ngagelan, Taman Nasional Alas Purwo (TNAP), dan di sekitar Penangkaran Penyu Semi Alami (PPSA), Banyuwangi, Indonesia. Penelitian dilakukan bulan Januari-Desember 2019. Jenis penelitian adalah kuantitatif dan kualitatif. Data yang diperoleh dianalisis secara deskriptif kulitatif dan korelasi. Hasil penelitian menginformasikan bahwa V. salvator mengunjungi daerah ini hanya pada musim penyu bertelur; mereka melakukan perjalanan ke daerah lain ketika bukan musim penyu bertelur. Lepidochelys olivacea mendominasi jenis penyu yang mendarat di TNAP. Sebanyak 1.056 L. Olivacea, 6 Chelonia mydas, Eretmochelys imbricata, dan Dermochelys coriacea masing-masing hanya satu satwa mendarat untuk bertelur. Pendaratan terjadi pada bulan April hingga Agustus, puncak pendaratan pada bulan Juni. V. salvator ditemukan berkeliaran di daerah ini mulai bulan April, memuncak pada minggu kedua Agustus dan menurun bulan Oktober. Perbedaan waktu pendaratan dan perilaku kedatangan V. salvator berkaitan dengan masa inkubasi telur penyu berkisar antara 40–50 hari. Bau telur penyu dan tukik mati mengundang V. salvator ke kawasan ini. Perilaku ini karena V. salvator memiliki indera penciuman yang kuat, terdeteksi oleh lidah yang sering dijentikkan.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.26962
      Issue No: Vol. 16, No. 2 (2023)
       
  • Potensi Cendawan Xylaria sp. Sebagai Sumber Antioksidan

    • Authors: Handika Dwi Prasetyo, Sri Listiyowati, Irmanida Batubara
      Pages: 365 - 375
      Abstract:  AbstrakPencegahan radikal bebas di dalam tubuh dapat dilakukan dengan menggunakan antioksidan. Cendawan Xylaria memiliki kandungan senyawa bioaktif yang berasal dari metabolit sekunder yang berpotensi sebagai sumber antioksidan alami baru. Penelitian ini bertujuan menentukan potensi Xylaria sp. (strain F, D, C) sebagai sumber antioksidan melalui pengukuran aktivitas antioksidan dan kandungan total flavonoidnya. Cendawan ditumbuhkan pada media Potato Dextrose Yeast Extract Broth (PDYEB) dan diinkubasi 14 hari dengan kondisi gelap dan statis. Miselium cendawan digerus dengan bantuan nitrogen cair, kemudian ekstraksi dilakukan menggunakan pelarut metanol sebanyak dua kali ulangan. Penentuan aktivitas antioksidan menggunakan metode 2,2-Diphenyl-1-picrylhydrazyl (DPPH) dan kandungan total flavonoid ditentukan menggunakan metode alumunium klorida (AlCl3) yang dinyatakan ekuivalen kuersetin (QE). Seluruh sampel Xylaria sp. memiliki aktivitas antioksidan yang lemah dan kandungan flavonoid yang juga rendah. Xylaria sp. strain F memiliki aktivitas antioksidan tertinggi sebesar 1915,14 ± 24,73 µg/mL dan Xylaria sp. strain D memiliki kandungan total flavonoid tertinggi sebesar 2,41 ± 0,09 mg QE/g ekstrak. Senyawa flavonoid pada sampel Xylaria sp. tidak menjadi senyawa utama yang menunjukkan aktivitas antioksidannya.AbstractPrevention of free radicals in the body can be done by using antioxidants. Xylaria fungus contains bioactive compounds derived from secondary metabolites that have the potential as a source of new natural antioxidants. This study aims to determine the potential of Xylaria sp. (strains F, D, C) as a source of antioxidants by measuring their antioxidant activity and total flavonoid content. The fungus was grown on Potato Dextrose Yeast Extract Broth (PDYEB) and incubated for 14 days in dark and static conditions. The mycelium of the fungus was crushed with the help of liquid nitrogen, then the extraction was carried out using methanol as a solvent for two repetitions. Antioxidant activity was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method and the total flavonoid content was determined using the alumunium chloride (AlCl3) method which is expressed as quercetin equivalent (QE). All samples of Xylaria sp. have the weakest antioxidant activity and lowest flavonoid content. Xylaria sp. strain F had the highest antioxidant activity of 1915,14 ± 24,73 µg/mL and Xylaria sp. strain D had the highest total flavonoid content of 2,41 ± 0,09 mg QE/g extract. The flavonoid compounds in the sample Xylaria sp. did not become the main compound showing antioxidant activity.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.27386
      Issue No: Vol. 16, No. 2 (2023)
       
  • Analisis Ekspresi Gen MmCu/Zn-SOD dan Ketahanan Tanaman Kentang Kultivar
           IPB CP3 Transgenik Terhadap Cekaman Herbisida Parakuat

    • Authors: Muhammad Akbar Velayati, Suharsono Suharsono, Miftahudin Miftahudin
      Pages: 376 - 385
      Abstract:  AbstrakPenggunaan herbisida parakuat untuk mengendalikan pertumbuhan gulma telah menjadi metode yang paling umum. Namun, penggunaan herbisida juga dapat menyebabkan kematian sel tanaman kentang yang sedang dibudidayakan, karena herbisida parakuat dapat menginduksi pembentukan senyawa Reactive Oxygen Species (ROS) yang dapat merusak sel tanaman. Ekspresi berlebih dari gen MmCu/Zn-SOD diharapkan dapat memberikan ketahanan tanaman terhadap cekaman herbisida parakuat. Penelitian ini bertujuan untuk menganalisis ekspresi gen MmCu/Zn-SOD pada kentang transgenik IPB CP3 kultivar dan ketahanannya terhadap cekaman herbisida parakuat. Perlakuan herbisida parakuat dengan konsentrasi 75 µM pada tanaman transgenik (P3SOD6, P3SOD9, P3SOD17) dan non-transgenik (NT) secara in vitro menunjukkan bahwa semua tanaman transgenik lebih tahan terhadap cekaman parakuat dibandingkan tanaman non-transgenik. Klon P3SOD9 paling tahan terhadap herbisida parakuat, dengan perbandingan 10 tanaman hidup dari 12 tanaman yang diuji. Ekspresi gen MmCu/Zn-SOD pada galur transgenik lebih tinggi dibandingkan galur non-transgenik. Ekspresi relatif gen MmCu/Zn-SOD pada P3SOD9, P3SOD6, dan P3SOD17 secara berturut-turut lebih tinggi 233,22; 127,62; dan 3,18 kali dibandingkan ekspresi gen tersebut pada NT. Semakin tinggi ekspresi gen MmCu/ZnSOD, semakin tinggi ketahanan terhadap cekaman herbisida parakuat.AbstractThe use of paraquat herbicide to control weed growth has become the most common method. However, using the herbicide can also cause cell death in potato plants that are being cultivated because it can induce the formation of Reactive Oxygen Species (ROS) compounds that can damage plant cells. Overexpression of the MmCu/Zn-SOD gene is expected to provide resistance to paraquat herbicide stress. The study aimed to analyze the expression of the MmCu/Zn-SOD gene in transgenic potato cultivar IPB CP3 and its resistance to paraquat herbicide stress. In vitro treatment of transgenic (P3SOD6, P3SOD9, P3SOD17) and non-transgenic (NT) potato with 75 µM paraquat herbicide showed that all transgenic plants were more resistant to paraquat stress than that non-transgenic plants. The P3SOD9 clone was the most resistant to paraquat herbicide, with a ratio of 10 surviving plants of the 12 tested plants. The expression of MmCu/Zn-SOD in transgenic lines was higher than that in non-transgenic lines. The relative expressions of MmCu/Zn-SOD gene in P3SOD9, P3SOD6, and P3SOD17 were 233.22; 127.62; and 3.18 times higher than that of NT, respectively. The expression level of MmCu/ZnSOD correlates with the level of resistance to paraquat herbicide stress.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.27468
      Issue No: Vol. 16, No. 2 (2023)
       
  • Diet, Feed Preferences, and Nutritional Intake of Hylobates albibarbis in
           Transit Cage BKSDA Kalimantan Tengah

    • Authors: Adisty Virakawugi Darniwa, Ida Kinasih, Nurul Aulia Fitri
      Pages: 386 - 394
      Abstract: AbstractHylobates albibarbis is a type of small primate that lives on the island of Borneo with a characteristic black face and white hair on the eyebrows, cheeks, and chin that resembles a beard. H. albibarbis feeding management is important in conservation efforts on ex-situ conservation because of animal welfare. This study aims to determine the composition of the diet, feed preferences and nutritional intake of feed given to H. albibarbis in transit cage at the Palangka Raya-Central Kalimantan Office for Conservation of Natural Resources (named Balai Konservasi Sumber Daya Alam-BKSDA) under the Directorate of Conservation of Natural Resources and Ecosystems of the Ministry of Environment & Forestry of the Republic of Indonesia. The methods used are focal animal sampling and restricted feeding observed in individual male adult and infant of H. albibarbis. The observations show that the feed preferred by adult H. albibarbis was the Ambon banana (98.21%) and the least preferred was the Kepok banana (74.26%). Otherwise, in infants H. albibarbis the most preferred feed was papaya (93.43%), and the least preferred feed was Ambon banana (58.10%). The average daily feed intake for adult H. albibarbis was 658.52 g, and for infant was 378.16 g. H. albibarbis in transit cage at the Palangka Raya BKSDA office, Central Kalimantan had good growth and healthy physical condition assumed from their body weight and length.AbstrakHylobates albibarbis merupakan kera kecil yang hidup di Pulau Kalimantan dengan ciri khas wajah berwarna hitam dan rambut berwarna putih pada alis, pipi, dan dagu yang menyerupai janggut. Pengelolaan pakan H. albibarbis penting dalam upaya konservasi dengan konservasi ex-situ untuk kesejahteraan hewan. Penelitian ini bertujuan untuk mengetahui komposisi pakan, preferensi pakan, dan asupan nutrisi pakan yang diberikan pada H. albibarbis di kandang transit kantor BKSDA Palangka Raya Kalimantan Tengah. Metode yang digunakan adalah focal animal sampling dan restricted feeding pada individu jantan dewasa dan jantan bayi H. albibarbis. Observasi dilakukan dalam durasi 12 jam selama 31 hari. Hasil pengamatan menunjukkan bahwa pakan yang disukai H. albibarbis dewasa adalah pisang Ambon (98,21%) dan yang tidak disukai adalah pisang Kepok (74,26%). Pakan yang disukai bayi H. albibarbis adalah pepaya (93,43%) dan yang tidak disukai adalah pisang Ambon (58,10%). Total asupan pakan harian untuk H. albibarbis dewasa adalah 658,52 g. Total asupan pakan harian untuk H. albibarbis bayi adalah 378,16 g. H. albibarbis di kandang transit kantor BKSDA Palangka Raya Kalimantan Tengah memiliki pertumbuhan yang baik dan kondisi fisik yang sehat berdasarkan berat badan dan panjang tubuhnya.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.27817
      Issue No: Vol. 16, No. 2 (2023)
       
  • Identification of Phytoplankton at Ephemeral Pond with Acidic pH in Bangka
           Regency

    • Authors: Andri Kurniawan, Ardiansyah Kurniawan, Robin Robin
      Pages: 395 - 401
      Abstract:  AbstractEphemeral waters, the temporary aquatic environment become an interesting habitat to explore extremophile organism, include phytoplankton. Furthermore, the waters have an acidic condition or low pH that impact to metabolisms, community structure, and diversity of phytoplankton. This study was conducted on June until August 2022 in Bangka Regency, Bangka Belitung Archipelago Province, Indonesia. We analyzed the phytoplankton presence at acidic ephemeral waters to indicated their potential as primary producer in food web, bioindicator, and ecological succession agent. This study was conducted by exploration method of phytoplankton diversity. The research observed and found five class and twelve genera that consist of class Chlorophyceae (genera Enteromorpha, Ankistrodesmus, Prasiola, Pleurococcus, and Coleochaete), class Rhodophyceae (genera Lemanea), class Diatoms (genera Diatoma, Synedra, and Navicula), class Xanthophyceae (genera Ophiocytium), and class Cyanobacteria (genera Oscillatoria and Anabaena). The class Chlorophyceae, genera Enteromorpha were the highest community at the both of acidic waters and they could survive at pH 3.52 + 0.5 to 3.71 + 0.8.AbstrakPerairan ephemeral, lingkungan perairan musiman menjadi suatu habitat yang menarik untuk mengeksplorasi organisme ekstremofil, termasuk fitoplankton. Lebih jauh lagi, perairan tersebut memiliki kondisi asam atau pH rendah yang berdampak pada metabolisme, struktur komunitas, dan diversitas fitoplankton. Penelitian ini dilaksanakan pada Juni hingga Agustus 2022 di Kabupaten Bangka, Provinsi Kepulauan Bangka Belitung, Indonesia. Kami menganalisis keberadaan fitoplankton di perairan ephemeral asam untuk mengindikasikan potensi fitoplankton sebagai produsen utama, bioindikator dan agen suksesi lingkungan. Penelitian ini dilaksanakan dengan metode eksplorasi diversitas fitoplankton. Penelitian ini mengobservasi dan menemukan lima kelas dan dua belas genus yang terdiri atas kelas Chlorophyceae (genus Enteromorpha, Ankistrodesmus, Prasiola, Pleurococcus, dan Coleochaete), kelas Rhodophyceae (genus Lemanea), kelas Diatoms (genus Diatoma, Synedra, dan Navicula), kelas Xanthophyceae (genus Ophiocytium), dan kelas Cyanobacteria (genus Oscillatoria dan Anabaena). Kelas Chlorophyceae, genus Enteromorpha adalah komunitas tertinggi pada kedua perairan asam dan mampu bertahan pada pH 3.52 + 0.5 to 3.71 + 0.8.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.28608
      Issue No: Vol. 16, No. 2 (2023)
       
  • Growth And Lipid Accumulation of Chaetoceros calcitrans After Phosphorus
           And Light Intensity Optimization

    • Authors: Rizal Maulana Hasby, Ismi Farah Syarifah, Opik Taufiqurrohman
      Pages: 402 - 411
      Abstract: AbstractGrowth and lipid content of Chaetoceros calcitrans are greatly influenced by environmental factors. The aims of this study to optimize phosphorus concentrations and light intensity on the growth and lipid accumulation of C. calcitrans. This study used N:P:light intensity concentration from the previous research, namely 441:36.2 µM:2,500 lux (12:1:2,500 lux). Concentrations of P were then optimized to 36.2 µM, 27.5 µM, 18.1 µM, 9.05 µM (1; 0.75; 0.5; 0.25) and light intensity to 2,500; 3,000; 3,500; 4,000 lux. C. calcitrans was cultured in medium f/2 guillard, the initial density was 6 x 105 cells/mL. Sampling for lipid analysis was conducted in exponential, stationary, and the end of stationary phase by centrifugation, whereas lipid was extracted using the Bligh and Dyer method, and dried lipids were analyzed using gas chromatography-GC. The highest lipid content found at the late stationary phase of the N:P concentrations and light intensity 12:0.5:(4,000 lux), there was 15.46 ± 0.53%-dw with the highest cell density of 5.5 ± 5.56 x 106 cells/mL. The analysis result showed that palmitoleic acid (C16:1) was the highest fatty acid produced by each optimization. Nutritional deficiency and high light intensity were triggers for of C. calcitrans to accumulate lipids, and influence the fatty acid profile of C. calcitrans.AbstrakPertumbuhan dan kandungan lipid Chaetoceros calcitrans sangat dipengaruhi oleh faktor lingkungan. Tujuan penelitian ini adalah untuk mengoptimasi konsentrasi fosfor dan intensitas cahaya terhadap pertumbuhan dan akumulasi lipid C. calcitrans. Penelitian ini menggunakan hasil konsentrasi N:P:intensitas cahaya dari penelitian sebelumnya, yaitu 441:36.2µM:2,500 lux (12:1:2,500 lux). Konsentrasi P kemudian dioptimasi menjadi 36,2 µM, 27,5 µM, 18,1 µM, 9,05 µM (1; 0,75; 0,5; 0,25), dan intensitas cahaya menjadi 2.500; 3.000; 3.500; 4.000 lux. C. calcitrans dikultur dalam medium f/2 guilard, densitas awal 6 x 105 sel/mL. Pengambilan sampel untuk analisis lipid dilakukan pada fase eksponensial, stasioner, dan akhir stasioner dengan sentrifugasi, sedangkan lipid diekstraksi menggunakan metode Bligh dan Dyer, lipid kering dianalisis menggunakan kromatografi gas-GC. Kandungan lipid tertinggi terdapat pada fase akhir stasioner konsentrasi N:P dan intensitas cahaya 12:0,5:(4,000 lux), yaitu sebesar 15,46 ± 0,53%-dw dengan kerapatan sel tertinggi 5,5 ± 5,56 x 106 sel/mL. Hasil analisis menunjukkan bahwa asam palmitoleat (C16:1) merupakan asam lemak tertinggi yang dihasilkan oleh masing-masing optimasi. Kekurangan nutrisi dan intensitas cahaya yang tinggi menjadi pemicu C. calcitrans. mengakumulasi lipid, dan mempengaruhi profil asam lemak C. calcitrans.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.30316
      Issue No: Vol. 16, No. 2 (2023)
       
  • Fish Community Structure The Dry Season in The Cipeles River, Sumedang
           Regency, West Java Province, Indonesia

    • Authors: Ayi Yustiati, Rostika Rostika, Ibnu Bangkit Bioshina Suryadi, Titin Herawati
      Pages: 412 - 422
      Abstract: AbstractCipeles River is a sub-watershed of the Cimanuk River that flows in Sumedang Regency, Indonesia. The upstream is located in Sukasari District and flows into Tomo District. This study aimed to determine the fish community structure in the Cipeles River during the dry season. This research was conducted from September to December 2020 using field observation methods and purposive sampling techniques at three stations (Station 1 at 6°48′59.5″S and 108°01′22.5″E; Station 2 at 6°48′56.2″S and 108°01′32.3″E; and Station 3 at 6°47′3.7″S and 108°05′44″E) data analysis employed descriptive-comparative. Parameters observed are abundance, diversity, dominance, uniformity, community structure, and water quality. The results showed that from 566 caught fish the diversity index is low with a value of 0.86–0.89, a moderate dominance index with a value of 0.55–0.62, and a low uniformity index of 0.16–0.17. Based on the three index values, it can be concluded that the fish community structure the dry season in the Cipeles River is categorized as depressed, caused by the high influence of total suspended solids that have exceeded the water quality threshold value for fisheries (according to regulation of the Government of the Republic of Indonesia Number 22 of 2021 on Spatial Management) with concentrations ranging from 67 mgL-1 to 74 mgL-1.AbstrakSungai Cipeles merupakan sub DAS dari Sungai Cimanuk yang mengalir di Kabupaten Sumedang, Indonesia. Hulunya terletak di Kecamatan Sukasari dan mengalir ke Kecamatan Tomo, Kabupaten Sumedang. Penelitian ini bertujuan untuk mengetahui struktur komunitas ikan di Sungai Cipeles pada musim kemarau. Penelitian dilakukan pada bulan September hingga Desember 2020 dengan menggunakan metode pengamatan lapangan dan teknik purposive sampling pada tiga stasiun (Stasiun 1 di 6°48′59.5″LS dan 108°01′22.5″BT, Stasiun 2 di 6°48′56.2″ S dan 108°01′32.3″BT, dan Stasiun 3 di 6°47′3.7″LS dan 108°05′44″E). Analisis data menggunakan deskriptif-komparatif. Parameter yang diamati adalah kelimpahan, keragaman, dominasi, keseragaman, struktur komunitas, dan kualitas air. Hasil penelitian menunjukkan bahwa dari 566 ikan yang ditangkap indeks keragamannya rendah dengan nilai 0,86–0,89, indeks dominansi sedang dengan nilai 0,55–0,62, dan indeks keseragaman rendah 0,16–0,17. Berdasarkan ketiga nilai indeks tersebut dapat disimpulkan bahwa struktur komunitas ikan pada musim kemarau di Sungai Cipeles dikategorikan tertekan, disebabkan oleh tingginya pengaruh total padatan tersuspensi yang telah melebihi nilai ambang batas kualitas air untuk perikanan (menurut Peraturan Pemerintah Republik Indonesia Nomor 22 Tahun 2021 tentang Penataan Ruang) dengan konsentrasi berkisar antara 67 mgL-1 sampai dengan 74 mgL-1.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.30665
      Issue No: Vol. 16, No. 2 (2023)
       
  • Optimization of Composting Growing Media Time and Rice Husk Addition in
           Auricularia auricula Cultivation

    • Authors: Dionysius Subali, Vasya Theodora Givianty, Anastasia Tatik Hartanti, Listya Utami Karmawan
      Pages: 423 - 433
      Abstract:  AbstractAuricularia auricula is a wood mushroom that is commonly consumed and is in demand by the people as a food ingredient because it contains many nutrients and health benefits. Cultivation of A. auricula was carried out using growing media and controlled environmental conditions. This study objective to determine the optimal combination of composting time and the rice husks addition in the A. auricula production. The treatment in this study was the composting time of the growing media for 0, 2, 4, 6, and 8 days, as well as the addition of rice husks as much as 0, 25, and 50%. This research consisted of the preparation of growing media, spawning and incubation of A. auricula, and harvesting of fruit bodies. The success of mushroom cultivation is seen from the value of biological efficiency in each treatment. The combination treatment of 8 days composting time with the addition of 50% rice husk resulted in A. auricula production as much as 52.37%, while the combination of 4 days composting time treatment and the addition of 25% rice husks resulted in the best production of A. auricula, as it produced the mushroom weight of 340 ± 16.95 g with biological efficiency value as much as 68% for five harvest durations within four months after first watering. The treatment of composting media tie and the addition of rice husks to A. auricularia’s were successfully carried out.AbstrakAuricularia auricula merupakan jamur kayu yang umum dikonsumsi dan diminati oleh masyarakat sebagai bahan pangan karena mengandung nutrisi dan banyak manfaat. Budi daya A. auricula dilakukan dengan menggunakan media tanam dalam kondisi lingkungan yang terkontrol. Penelitian ini bertujuan menentukan kombinasi perlakuan waktu pengomposan dan penambahan sekam padi yang optimum terhadap produksi A. auricula. Perlakuan pada penelitian ini adalah waktu pengomposan media tanam selama 0, 2, 4, 6, dan 8 hari, serta penambahan sekam padi sebanyak 0, 25, dan 50%. Penelitian ini tersusun atas pembuatan media tanam, pembibitan dan inkubasi A. auricula, dan pemanenan tubuh buah. Keberhasilan budi daya jamur dilihat dari nilai efisiensi biologi pada setiap perlakuan. Kombinasi perlakuan pengomposan 8 hari dengan penambahan sekam padi 50% menghasilkan produksi A. auricula sebesar 52,37%, sedangkan kombinasi perlakuan waktu pengomposan 4 hari dan penambahan sekam padi 25% menghasilkan produksi A. auricula terbaik karena menghasilkan berat jamur sebanyak 340 ± 16,95 g dengan nilai efisiensi biologi sebesar 68% selama lima durasi panen dengan waktu empat bulan setelah dilakukan penyiraman pertama. Secara keseluruhan, perlakuan waktu pengomposan media dan penambahan sekam padi pada media pertumbuhan A. auricula berhasil dilakukan.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.30991
      Issue No: Vol. 16, No. 2 (2023)
       
  • Feed Management and Nutritional Status of Gibbons (Symphalangus
           Syndactylus Raffles, 1821) at Tegal Alur Animal Rescue Center, Jakarta

    • Authors: Fahma Wijayanti, Abdul Bagas Al katiri, Nurul Handayani, Narti Fitriana
      Pages: 434 - 443
      Abstract:  AbstractGibbon (Symphalangus syndactylus Raffles, 1821) is an endangered species of black long-armed ape that is protected by national and international regulations. This study aims to analyze feeding behavior, feed management and nutritional status of gibbons in Tegal Alur Animal Rescue Center. The study was conducted in March-July 2020, using focal animal sampling and ad libitum sampling methods to 6 individual gibbons. Food management data collection includes information on feeding and the amount of feed, while nutritional status includes physical characteristics of the body, anthropometry, and analysis of feed composition. The results showed that the feeding schedule for gibbons was in accordance with the feeding times of gibbons in nature. Tegal Alur PPS provides food in the form of nine types of fruit, two types of vegetables and one type of leaves. Gibbons at Tegal Alur PPS Alur Animal Rescue Center has met the amount of feed consumption according to body weight. The nutritional status based on anthropometry shows that the body weight of the gibbon is not in accordance with its natural habitat. Morphological observations showed that the gibbon’s teeth and eyes were healthy, while some gibbons had hair loss and depigmentation.AbstrakOwa (Symphalangus syndactylus Raffles, 1821) merupakan spesies kera hitam lengan panjang yang terancam punah dan dilindungi oleh peraturan nasional dan internasional. Penelitian bertujuan untuk menganalisis perilaku makan, pengelolaan pakan, dan status gizi owa di Pusat Penyelamatan Satwa (PPS) Tegal Alur. Penelitian dilakukan pada bulan Maret-Juli 2020, dengan metode focal animal sampling dan ad libitum sampling pada 6 individu owa. Pendataan pengelolaan pakan meliputi informasi pakan dan jumlah pakan, sedangkan status gizi meliputi ciri fisik tubuh, antropometri, dan analisis komposisi pakan. Hasil penelitian menunjukkan bahwa jadwal pemberian pakan owa sesuai dengan waktu pemberian pakan owa di alam. PPS Tegal Alur menyediakan makanan berupa sembilan jenis buah, dua jenis sayuran dan satu jenis daun-daunan. Owa di Pusat Penyelamatan Satwa Tegal Alur telah memenuhi jumlah konsumsi pakan sesuai dengan berat badan. Status gizi berdasarkan antropometri menunjukkan bahwa berat badan owa lebih rendah dengan berat badan Owa di habitat aslinya. Pengamatan morfologi menunjukkan bahwa gigi dan mata owa dalam keadaan sehat, sementara beberapa owa mengalami kerontokan rambut dan depigmentasi.
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.33624
      Issue No: Vol. 16, No. 2 (2023)
       
  • Akumulasi Logam Timbal (Pb) Pada Tanaman Bayam (Amaranthus tricolor L.)
           Dengan Aplikasi Pupuk Mikoriza

    • Authors: Etyn Yunita, Dasumiati Dasumiati, Azizah Mei Widyastuti, Irzal Irda
      Pages: 444 - 455
      Abstract:  AbstrakPencemaran tanah oleh logam timbal (Pb) merupakan salah satu bentuk pencemaran yang sangat berbahaya bagi mahluk hidup. Salah satu tanaman bioakumulator penyerap logam berat di lingkungan adalah tanaman bayam (Amaranthus tricolor L.). Tujuan dari penelitian ini adalah untuk menganalisis pengaruh aplikasi pupuk mikoriza terhadap akumulasi Pb pada akar, batang, dan daun serta pertumbuhan tanaman bayam. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan dosis pupuk mikoriza, yaitu 0 g, 5 g, 10 g, dan 15 g per polybag. Jumlah Pb yang diaplikasikan ke media tanam adalah 5 ppm per polybag. Akumulasi Pb tertinggi terdapat pada tanaman bayam dengan perlakuan 5 g pupuk mikoriza. Rata-rata akumulasi Pb di akar, batang, dan daun pada perlakuan ini berturut-turut adalah 103,57 ppm; 36,67 ppm; dan 8,60 ppm. Pertumbuhan tanaman bayam pada perlakuan 5 g pupuk mikoriza lebih baik dari perlakuan lainnya, yaitu memiliki rata-rata tinggi tanaman dan jumlah daun tertinggi pada minggu ke-4 (9,7–12,5 cm dan 6,9–8,6 helai). Aplikasi pupuk mikoriza dapat meningkatkan akumulasi Pb pada akar, batang, dan daun serta meningkatkan pertumbuhan tanaman bayam pada dosis 5 g per polybag.AbstractSoil pollution by lead (Pb) is a form of pollution that is very dangerous for living creatures. One of the bioaccumulator plants that absorb heavy metals in the environment is spinach (Amaranthus tricolor L.). The aim of this research was to analyze the effect of mycorrhizal fertilizer application on Pb accumulation in roots, stems and leaves as well as spinach plant growth. This research used a completely randomized design (CRD) with treatment doses of mycorrhizal fertilizer, namely 0 g, 5 g, 10 g, and 15 g per polybag. The amount of Pb applied to the planting media is 5 ppm per polybag. The highest Pb accumulation was found in spinach plants treated with 5 g of mycorrhizal fertilizer. The average accumulation of Pb in roots, stems and leaves in this treatment was 103.57 ppm; 36.67 ppm; and 8.60 ppm. The growth of spinach plants in the 5 g mycorrhizal fertilizer treatment was better than the other treatments, namely having the highest average plant height and number of leaves in the 4th week (9.7-12.5 cm and 6.9-8.6 pieces). Application of mycorrhizal fertilizer can increase Pb accumulation in roots, stems and leaves and increase the growth of spinach plants at a dose of 5 g per polybag. 
      PubDate: 2023-10-27
      DOI: 10.15408/kauniyah.v16i2.35282
      Issue No: Vol. 16, No. 2 (2023)
       
 
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