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Visnyk of Dnipropetrovsk University. Biology, medicine
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This is an Open Access Journal

Open Access journal
ISSN (Print) 2310-4155 - ISSN (Online) 2312-7295
Published by Oles Honchar Dnipropetrovsk National University

[7 journals]

Effects of gonadectomy on clinical-hematological, metabolic and hormone
conditions of cockerels
- Authors: М. О. Zakharenko, І. М. Cheverda, І. М. Kurbatova
  Pages: 10 - 14
  Abstract: Gonadectomy (caponization) of cockerels is an important way of increasing their productivity, improving the quality and taste properties of the meat, and obtaining dietary products. The objective of the study was the influence of gonadectomy of cockerels on their clinical condition, morphological composition of blood, hemoglobin content and anabolism in the tissues, their productivity and chemical content of the muscles. In the experiment, we used 30 Adler silver cockerels aged 4 weeks, which were divided into two groups: control (intact) and experimental (gonadectomized) according to the analogue principle, with 15 individuals in each. The experiment lasted for 185 days, including the main period of 175 days. We determined that the body temperature of cockerels after gonadectomy increased as early as days one and three. In the blood of gonadectomized cockerels, the number of leukocytes decreased by 36.3%, thrombocytes – by 24.7%, while hemoglobin concentration, numbers of heterophils, eosinophils, basophils, monocytes and lymphocytes did not change compared with the intact individuals. On days 20 and 185 of the experiment, the concentration of testosterone in the blood plasma of gonadectomized cockerels was lower respectively by 57.1% and 53.1%, whereas no differences were found prior to gonadectomy and on the third day of the experiment. We determined increase in uric acid concentration and decrease in the level of ionizing calcium in blood plasma of gonadectomized cockerels compared with intact individuals, while the content of cortisol, glucose, total protein, triglycerides, cholesterol, total calcium and inorganic phosphorus, and also activities of alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase did not change. Live weight of gonadectomized cockerels in the period from 45 to 105 days of the raising did not change, and was higher by 10.4–17.0% from day 115 to 145, and further remained not different from the control. Thoracic muscles of gonadectomized cockerels contained 1.8 times more fat, while the levels of moisture, dry matter, protein and ash in thoracic and thigh muscles did not change. The conducted studies have revealed the effect of gonadectomy of roosters on clinical-hematological parameters, metabolic processes, productivity of birds and chemical composition of muscles and may be used to improve the production of chicken meat with high dietary and taste properties.
  PubDate: 2022-01-11
  DOI: 10.15421/022202
  Issue No: Vol. 13, No. 1 (2022)
Influence of nanosilver in hybrid carriers on morphological and
biochemical blood pa-rameters of laying hens
- Authors: L. V. Shevchenko, Y. Y. Dovbnia, N. М. Permyakova, Т. B. Zheltonozhskaya, S. V. Shulyak, D. O. Klymchuk
  Pages: 15 - 22
  Abstract: The search for an alternative to antibiotics in poultry has led to a study of the effectiveness of using nanosilver preparations in the production of table eggs. The experiment determined the effect of the drug nanosilver in carriers based on polymer/inorganic hybrids (AgNPs/SPH) on morphological and biochemical parameters of the blood of laying hens. For this, 45 Hy-Line W36 hens were used at the age of 38 weeks, which were randomly divided into three groups. The AgNPs/SPH solution was administered 3 times a month with an interval of 10 days at concentrations of 0.0, 1.0, and 2.0 mg/L (0.0, 0.2 and 0.4 mg per hen per day). The introduction of AgNPs/SPH in doses of 0.2 and 0.4 mg per hen per day three times a month did not have a significant effect on the morphological parameters of the blood. A single dose of 0.2 mg AgNPs/SPH solution per hen per day increased the level of total protein, glucose, cholesterol, as well as the activity of alanine aminotransferase and alkaline phosphatase in the blood serum and decreased albumin, creatinine and gamma-glutamyl transpeptidase activity. Feeding laying hens a solution of nanosilver in a larger dose had a less pronounced effect on these indicators. Two-fold administration of AgNPs/SPH solution at a dose of 0.2 mg per laying hen per day increased only gamma-glutamyl transpeptidase activity in the blood serum, but decreased the level of total activity of protein, albumin, phosphorus, and alkaline phosphatase. At the same time, the drug nanosilver in double dose per day caused an increase in albumin content and alkaline phosphatase activity in the serum of hens. Triple feeding of laying hens with a solution of nanosilver at a dose of 0.2 mg per hen per day did not affect most of the biochemical parameters of serum, but in the double dose increased the content of total protein against the background of lowered cholesterol and gamma-glutamyl transpeptidase activity. With the increase in the frequency of feeding laying hens solutions of nanosilver in carriers based on polymer / inorganic hybrids, the level of severity of their impact on the metabolic profile of serum decreased. The results of research can be the basis for determining the optimal interval of application of nanosilver drugs in poultry, depending on the method of their synthesis and stabilization.
  PubDate: 2022-01-14
  DOI: 10.15421/022203
  Issue No: Vol. 13, No. 1 (2022)
Dissimilatory reduction of sulfate, nitrate and nitrite ions by bacteria
Desulfovibrio sp. under the influence of potassium dichromate
- Authors: O. M. Moroz, S. O. Hnatush, G. V. Yavorska, G. I. Zvir
  Pages: 23 - 37
  Abstract: In the process of anaerobic respiration, sulfate reducing bacteria, besides sulfates, can use other electron acceptors: nitrates, nitrites, oxidized forms of heavy metals, in particular, hexavalent chromium, which are harmful for organisms. Selection of pollutant-resistant stains of this kind of bacteria isolated from technogenically altered ecotopes, capable of reductive transformation of various nature pollutants, is an especially relevant task for the creation of new effective remediation biotechnologies. The purpose of this work was to investigate the regularities of usage of sulfate, nitrate or nitrite ions by bacteria of the Desulfovibrio genus, isolated from Yavorivske Lake, at conditions of simultaneous presence in the medium of another electron acceptor – Cr(VI), to establish a succession of electron acceptors’ reduction by investigated sulfidogenic bacteria and to evaluate the efficiency of their possible application in technologies of complex purification of the environment from metal, sulfur and nitrogen compounds. Bacteria were grown under anaerobic conditions for 10 days in Kravtsov-Sorokin medium without Mohr’s salt. To study the efficiency of sulfate, nitrate, or nitrite ions’ reduction at simultaneous presence in the medium of Cr(VI), bacteria were sown in media with Na2SO4×10H2O, NaNO3, NaNO2 or K2Cr2O7 to final SO42–, NO3–, NO2– or Cr(VI) concentration in the medium of 3.47 (concentration of SO42– in medium of standard composition) or 1.74, 3.47, 5.21, 6.94, 10.41 mM. Biomass was determined turbidimetrically, and the concentrations of sulfate, nitrate, nitrite, ammonium ions, hydrogen sulfide, Cr(VI), Cr(ІІІ) in cultural liquid were determined by spectrophotometric method. It has been established that Cr(VI) inhibits the biomass accumulation, sulfate ions’ reduction and hydrogen sulfide production by Desulfovibrio sp. after simultaneous introduction into the medium of 3.47 mM SO42– and 1.74–10.41 mM Cr(VI). In the medium with the same initial content (3.47 mM) of SO42– and Cr(VI), bacteria reduced 2.1–2.3 times more Cr(VI) than sulfate ions with Cr(III) production at concentrations up to 2.2 times higher than hydrogen sulfide. It has been shown that K2Cr2O7 inhibits the biomass accumulation, the nitrate ions reduction and the ammonium ions production by bacteria after simultaneous addition into the medium of 3.47 mM NO3– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO3– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) of NO3– and Cr(VI) bacteria reduced 1.1–1.3 times more nitrate ions than Cr(VI) with the production of ammonium ions at concentrations up to 1.3 times higher than that of Cr(III). It has been established that K2Cr2O7 inhibits the biomass accumulation, the nitrite ions’ reduction and the ammonium ions’ production by bacteria after simultaneous addition into the medium of 3.47 mM NO2– and 1.74–10.41 mM Cr(VI) or 1.74–10.41 mM NO2– and 3.47 mM Cr(VI). In the medium with the same initial content (3.47 mM) NO2– and Cr(VI) the reduction of Cr(VI) by bacteria practically did not differ from the reduction of nitrite ions (was only slightly lower – up to 1.1 times), almost the same concentrations of trivalent chromium and ammonium ions in the cultural liquid were detected. The processes of nitrate and nitride reduction, carried out by bacteria of Desulfovibrio genus, were revealed to be less sensitive to the negative influence of sodium dichromate, as compared with the process of sulfate ions’ reduction, which in the medium with 3.47 mM SO42– and 1.74–10.41 mM Cr(VІ) decreased by 3.2–4.6 times as compared with this process in the medium with only Na2SO4×10H2O. The investigated strains of bacteria are adapted to high concentrations of toxic pollutants (up to 10.41 mM) and therefore are promising for application in technologies of complex environment purification from hexavalent chromium, sulfur and nitrogen compounds.
  PubDate: 2022-01-25
  DOI: 10.15421/022204
  Issue No: Vol. 13, No. 1 (2022)
Temperature, heat shock proteins and growth regulation of the bone tissue
- Authors: V. V. Kuibida, P. P. Kohanets, V. V. Lopatynska
  Pages: 38 - 45
  Abstract: Ambient heat modulates the elongation of bones in mammals, and the mechanism of such a plasticity has not been studied completely. The influence of heat on growth and development of bone depends on its values. Five zones of temperature influence on the bone tissue with different biological effects have been distinguished : a) under-threshold thermal zone < 36.6 ºС, insufficient amount of heat is a limiting factor for osteogenesis; b) normal temperature zone 36.6‒37.5 ºС, the processes of breakdown and development of bone in this temperature range is balanced; b) zone of mild thermal shock 39‒41 ºС, the processes of functioning of osteoblasts, osteocytes and formation of the bone tissue intensify; d) the zone of sublethal thermal shock > 42 ºС, growth of bone slows; e) zone of non-critical shock > 50 ºС, bone tissue cells die. We propose a model of the mechanism of influence of heat shock on bone growth. Mild heat shock is a type of stress to which membrane enzymes adenylyl cyclase and cAMP-protein kinase react. Protein kinase A phosphorylates the gene factors of thermal shock proteins, stress proteins and enzymes of energy-generating processes – glycolysis and lipolysis. Heat shock protein HSP70 activates alkaline phosphatase and promotes the process of mineralization of the bone tissue. In the cells, there is intensification in syntheses of insulin-like growth factor-I, factors of mitogenic action, signals of intensification of blood circulation (NO) and synthesis of somatotropin. The affinity between insulin-like growth factor I and its acid-labile subunit decreases, leading to increased free and active insulin-like growth factor I. Against the background of acceleration of the capillarization process, energy generation and the level of stimulators of growth of bone tissue, mitotic and functional activities of producer cells of the bone – osteoblasts and osteocytes – activate. The generally known Allen’s rule has been developed and expanded: “Warm-blooded animals of different species have longer distal body parts (tails) if after birth the young have developed in the conditions of higher temperature”. The indicated tendency is realized through increased biosynthesis of heat shock proteins and other stimulators of growth processes in the bone tissue.
  PubDate: 2022-02-21
  DOI: 10.15421/022205
  Issue No: Vol. 13, No. 1 (2022)
Spread of Aujeszky’s disease among wild boars and domestic pigs in
Ukraine
- Authors: V. V. Ukhovskyi, L. Y. Korniienko, A. V. Pyskun, O. M. Chechet, Z. M. Drozhzhe, I. M. Polupan, G. B. Aliekseieva, O. A. Moroz, O. M. Romanov
  Pages: 46 - 52
  Abstract: Aujeszky’s disease, also known as pseudorabies is an acute infectious viral disease in a variety of mammals caused by Suid herpesvirus 1, a member of the subfamily Alphaherpesvirinae and the family Herpesviridae. This virus causes significant economic losses in agriculture of numerous countries. In particular, in pig farming, the main losses are currently associated with restrictions of international trade, as well as the cost of vaccination or slaughter of infected animals. The main reservoirs species of this disease are considered to be wild boars and domestic pigs. So, in this article the authors performed a comparative retrospective analysis of the prevalence of Aujeszky’s disease among these species in Ukraine over 2009–2020 by systematizing and analyzing the epizootic data and results of serological studies. As a result of the conducted research, there is a clear trend that shows the reduction in the number of locations in Ukraine (farms, households, etc.) with domestic pigs affected by Aujeszky’s disease. Thus, in 2009 four such farms were found, and in 2018 – only one. During 2014–2016 and 2019–2020, affected farms and locations were not registered for this disease. In total, 21 such points with infected pigs were registered during the analyzed period. As for wild boars, during the analyzed 12 years, locations with animals affected by Aujeszky’s disease were registered only in 2013 and 2014 (two and one, respectively). It should be noted that all of those with infected wild boars were found in the AR Crimea. For the twelve years, the number of tested sera samples from domestic pigs amounted to 378,678 (7.4% were positive) and from wild boars – 9,052 (19.8% were positive). As a result of comparative geographical analysis of the obtained data on the detection of specific humoral antibodies to the Aujeszky’s disease virus among both species for 12 years in the regions of Ukraine, there is no established clear correlation between the spread of the disease among wild boars and domestic pigs. Thus, the highest seroprevalence rates among wild boars were observed in the northern and northeastern parts of Ukraine, and in domestic pigs, on the contrary, in the southern and central parts of this country. It should be noted, that since 2014 the number of serological tests among both species for carriers of Aujeszky’s disease has significantly decreased.
  PubDate: 2022-02-03
  DOI: 10.15421/022206
  Issue No: Vol. 13, No. 1 (2022)
Morphology and specifics of morphometry of lungs and myocardium of heart
ventricles of cattle, sheep and horses
- Authors: L. P. Horalskyi, М. R. Ragulya, N. M. Glukhova, I. M. Sokulskiy, N. L. Kolesnik, O. F. Dunaievska, B. V. Gutyj, I. Y. Goralska
  Pages: 53 - 59
  Abstract: Respiratory organs and cardiovascular system are interconnected and perform extremely vital functions of the organism, the main goals being performing gas exchange with the environment and emitting carbon dioxide produced in the organism into the environment. Therefore, we carried out comparative histo- and cytomorphometric evaluation of morphological structures of the heart and lungs of cattle, sheep and horses. Using complex methods of research, we determined specifics of microscopic structure and histometric parameters of parenchyma of the lungs and myocardium of the ventricles of the hearts of clinically healthy animals in species aspect – cattle, sheep and horses. The studies revealed that the histoarchitectonics of the studied animals were similar in structure, characteristic of other species of agricultural mammals, and distinct morphometric peculiarities of their histostructures. The study indicated that the respiratory zone of the lungs is most developed in horses. This parameter was lower in ruminants – cattle and sheep. Connective tissue septum of parenchyma of lungs was better expressed in cattle and sheep, and less in horses. At the same time, mean volume of lung alveoli in clinically healthy animals varied: being highest in horses – 699 ± 106 thou µm3, then cattle – 337 ± 43 thou µm3 and sheep – 158 ± 37 thou µm3. Such variation in histometric parameters of parenchyma of the lungs in experimental animals indicates adaptive specifics of the organism of animals in terms of living conditions. Therefore, the respiratory zone was most developed in the lungs of horses, animals that experience significant physical and physiological load on corresponding organs and systems. As a result of histometric studies of myocardium, we determined patterns of sizes of thickness of its cardiomyocytes and volume of their nuclei. We determined that in myocardium of the heart ventricles, in the same microscope field of view, cardiomyocytes of varying thicknesses occur. At the same time, thickness of cardiomyocytes, their mean volume of nuclei in the ventricle myocardium were expressed the most in cattle, then in horses and sheep, and their histometric parameters in myocardium of the left ventricle of the heart in the experimental animals were higher than in the right one. Therefore, thickness of cardiomyocytes of the left ventricle in cattle equaled 14.06 ± 0.41 µm, and volume of nuclei of cardiomyocytes respectively 124.55 ± 7.99 µm3. Similar changes in such parameters of histometry were found in sheep and horses. We attribute such varying histometric parameters of the thickness of cardiomyocytes and volume of their nuclei in myocardium of the left ventricle of the heart in experimental animals, compared with such parameters in the right ventricle, to the activities of the ventricles (the left one generally functions as a pump, right one – as a volumetric) and functional specifics of this myocardium tissue, which is capable of spontaneous rhythmic contractions, resulting in blood flow in the vessels: cardiomyocytes of the left ventricle carry greater load, promoting blood flow in vessels of greater (somatic) blood circulation, respectively cardiomyocytes of the right ventricle – less load, promoting blood flow in vessels of lesser (pulmonary) blood circulation.
  PubDate: 2022-01-26
  DOI: 10.15421/022207
  Issue No: Vol. 13, No. 1 (2022)
Hematological and biochemical parameters and resistance of the organism of
mother rabbits receiving sulfur compounds
- Authors: Y. V. Lesyk, A. Z. Dychok-Niedzielska, О. V. Boiko, О. F. Honchar, М. І. Bashchenko, І. І. Kovalchuk, B. V. Gutyj
  Pages: 60 - 66
  Abstract: Nanotechnologies are developing continuously, and their use is becoming more diverse and concrete, with high potential to improve the products of animal husbandry and animals in general. Yet, there have been few studies of nanotechnologies in those spheres. However, to confirm the efficiency and, mainly, safety of nanotechnologies, to avoid any harm to the environment or especially humans and animals, substantiated studies are still needed. A promising, but barely studied, direction of research is dosage of nanocompounds of biogenic elements in diets of animals, both modern supplements and alternatives for correcting mineral nutrition. Therefore, the objectives of the study presented here were changes in hematologic, biochemical and immune-biological parameters of the organism of mother rabbits under the influence of sulfur citrate and sodium sulfate for 14 days until insemination and until day 20 of lactation. The research was conducted on mother rabbits of the Hyla breed which were giving birth for the second time in LLC Horlytsia in Dobriany village of Horodok district of Lviv Oblast. Mother rabbits of the control group were fed ad libitum with complete granulated mixed feed with free access to water. Animals of experimental group I were fed feeds of the diet of the control group and during the day were watered sulfate citrate in dosage of 8 µg of S/kg of body weight. Females of experimental group II were fed the diet feeds of the control group and given sodium sulfate (Na2SO4) in the amount of 40 mg of S/kg of body weight. The mother rabbits received the supplements for 14 days both until insemination and for up to the 20th day of lactation. In the preparation period, on day 10 after the beginning of the study and on day 20 of lactation in the experimental period (65th day of watering the supplements), blood samples were taken from the marginal ear vein of the mother rabbits for hematological and biochemical studies. The study revealed that watering sulfate citrate in the dosage of 8 µg of S/kg of the body weight in the diet of mother rabbits for 14 days until insemination and up to day 20 of lactation increased the amount of erythrocytes by 19.5%, leukocytes by 37.5%, granulocytes by 38.3%, concentration of hemoglobin by 21.0%, mean corpuscular hemoglobin by 15.6%, and red cell distribution width by 14.7%, and activated protein metabolism, which manifested in 8.5% higher content of protein and activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase respectively by 12.9%, 29.6%, 19.8% and 51.8% lower content of triacylglycerols in the blood on day 20 of lactation compared with the control group. Intake of sulfur citrate heightened the parameters of immunobiological reactivity of the organisms of mother rabbits on day 65 of the experiment with significantly higher level of phagocytic number, phagocytic activity, lysozymic and bactericidal activities of blood serum, protein-associated hexoses, sialic acids and ceruloplasmin and immune globulins, compared with the control. Giving mother rabbits sodium sulfate in the amount of 40 mg/kg of body weight for 65 days led to less notable changes in the blood, expressed in 20.0% increase in mean corpuscular hemoglobin, higher phagocytic value, phagocytic activity, lysozymic and bactericidal activities of blood serum, protein-associated hexoses and ceruloplasmin. The results of the studies indicate possibility of additional use of supplement sulfur citrate in the amount of 8 µg of S/kg in diet of mother rabbits to improve metabolism, reproductive ability and immunological resistance in the period of increased physiological load.
  PubDate: 2022-04-16
  DOI: 10.15421/022208
  Issue No: Vol. 13, No. 1 (2022)
Effectiveness of food concentrate phenolic compounds of apples in
experimental membrane pathologies
- Authors: V. P. Fylymonenko, L. V. Galuzinska, G. B. Kravchenko, V. M. Kravchenko, Т. О. Bryukhanova, L. М. Мaloshtan, D. V. Lytkin
  Pages: 67 - 72
  Abstract: Apple fruits are an available source of phenolic compounds that exhibit a wide range of biological activities (antioxidant, anti-inflammatory, membrane stabilizing, etc.). The antioxidant properties of food concentrate phenolic compounds of apples (Concentrate) were studied in vitro in models of spontaneous and ascorbate induced lipid peroxidation (LPO) in rat liver homogenate, and acute carbon tetrachloromethane hepatitis was chosen as in vivo model in rats. Membrane stabilizing activity was evaluated by the degree of hemolysis in blood samples from the tail vein. The effect of Concentrate on vascular permeability was studied considering the time of animal skin papules staining at the site of injection of phlogogenic substances. Hepatoprotective activity in the model of acute carbon tetrachloride hepatitis was assessed by changes in prooxidant-antioxidant status in liver homogenate and liver enzymes activity in serum. Significant antioxidant effect of Concentrate was fixed in models of spontaneous and ascorbate induced LPO (TBA reactants’ content was 3.12 times and 2.25 times lower than control for spontaneous LPO and ascorbate induced LPO, respectively) and under tetrachloride hepatitis (Concentrate antioxidant activity was 47.8%). The membrane-protective activity of the studied Concentrate was also high and reached 50.1%. Also, Concentrate demonstrated capillary-strengthening properties, reducing the permeability of the vascular wall, which was caused by three different chlorogens, most notably by zymosan (Concentrate significantly delayed the stain utilization from the bloodstream by 2.14 times compared to control). Newly developed concentrate showed complex hepatoprotective activity, improving the indices of antioxidant-prooxidant status and activity of liver cytolysis enzymes in rats with tetrachloromethane hepatitis. The transparent corrective effects of Concentrate are the result of synergism and additivity of its multiple components and indicate the prospects of its further research in order to develop medications for the prophylaxis and treatment of diseases associated with membrane damage.
  PubDate: 2022-02-20
  DOI: 10.15421/022209
  Issue No: Vol. 13, No. 1 (2022)
Distribution of the nematodes of the genus Eustrongylides (Nematoda,
Dioctophymatidae) in the world
- Authors: S. L. Honcharov, N. M. Soroka, M. V. Halat, A. I. Dubovyi, V. V. Zhurenko, I. A. Halushko
  Pages: 73 - 79
  Abstract: Eustrongylides spp. are nematodes from the Dioctophymatidae family, potentially dangerous for the health of mammals. The aim of this review is to describe the distribution of the Eustrongylides spp. nematode. Eustrongylidosis is a parasitic disease which is common in countries with a continental, tropical or subtropical climate. Eustrongylides are biohelminths with a complicated development cycle. The nematode development takes place in the aquatic environment and includes a wide range of intermediate, definitive, and accidental hosts. The purpose of this review is describe the distribution of Eustrongylides spp. nematodes in the global regions, describe the prevalence of infection in those regions, and identify intermediate, definitive, and accidental hosts. It was found that Eustrongylides spp. nematrodes are significantly widespread around the world. In part in North America the agent of eustrongylidosis is registered mostly among birds and fishes, but cases of humans being affected with the disease are noted as well. In South America the given disease was also registered among amphibians and reptiles. In Europe, Asia, Africa, Oceania and countries of the Far East, the disease is registered mostly in fishes, rarely – in birds. The taxonomic identification showed that some nematode species were related to certain geographic areas. For example, Eustrongylides ignotus and Eu. tubifex were mostly registered in North America, while for Europe (including Ukraine), Near and Middle East, Eu. еxcisus is a dominant species. Analysis of scientific sources showed that continents with warmer climatic conditions (South America, Asia, etc.) are characterized with a wider range of fishes, birds and reptiles which can potentially serve as hosts for the Eustrongylides nematodes.
  PubDate: 2022-02-21
  DOI: 10.15421/022210
  Issue No: Vol. 13, No. 1 (2022)
Effects of aromatic compounds on movement activity of Pyrrhocoris apterus
in the conditions of a laboratory experiment
- Authors: V. V. Nehrii, V. V. Brygadyrenko
  Pages: 80 - 84
  Abstract: In the conditions of an urban environment, insects, including Pyrrhocoris apterus (Linnaeus, 1758) (Hemiptera, Pyrrhocoridae), are subject to dozens of volatiles: solvents, food flavorings, cosmetic products. For firebugs, some attract them, some scare them off, and to some they are indifferent. In this article, in the conditions of laboratory experiment, we evaluated the influence of chemical substances on rates of movement of firebugs. Under the influence of the substances we studied, there occurred no significant increase or decrease in the activity of firebugs. The most significant effect on rates of movement of firebugs was exerted by solvent, white spirit and solvent 646 p – firebugs travelled 12 cm distance for 24.2%, 49.6% and 58.7% of the time the control group did. Movement activity of firebugs was insignificantly decreased by aromatic extracts Royаl milk extract, Butcher’s broom extract and chrysalis oil: firebugs travelled 12 cm distance for 132.7%, 135.2% and 146.8% of time the control group. Aromatic substances likely interact with receptors on antennae of firebugs, and therefore the insects need additional time for orienting reactions, deciding whether those substances signal presence of potential food objects in the accessibility zone.
  PubDate: 2022-02-16
  DOI: 10.15421/022211
  Issue No: Vol. 13, No. 1 (2022)
Influence of winter water temperatures on the physiological state of carp
(Cyprinus carpio)
- Authors: L. V. Tsurkan
  Pages: 85 - 90
  Abstract: In the winter period, water temperature is one of the main factors influencing the physiological state of fish. Its optimal and stable indicator during the whole winter period guarantees high yield of fish and its quality. To this date, the winter period is characterized by elevated temperatures with acute fluctuations. In this work, the main object of study is carp (Cyprinus carpio) at the age of six (young-of-the-year) and ten (one-year) months. The aim of this study was to determine how much the period of the optimal winter water temperatures decreased and how this affected the weight and fatness, hematological profiles, erythrocyte indices and basic biochemical parameters of carp muscle tissue. As a result of research, it was found that optimal winter water temperatures decreased by an average of two months, which affected the physiological state of carp. Consequently, there was a decrease in body weight and fatness. Dissolved oxygen level, pH, nitrites, nitrates and hardness were normal, while oxidation was increased. Muscle fat and protein levels decreased to critical levels, at the same time, moisture and ash levels were noted to increase. The total content of red blood cells decreased, however, the level of hemoglobin, mean corpuscular hemoglobin (MCH), mean cell hemoglobin concentration (MCHC) increased. With increasing hemoglobin content and concentration, mean corpuscular volume (MCV) decreased. After wintering, the number of white blood cells increased. The biochemical profile of carp blood showed a decrease in total protein in blood serum, albumin, triglycerides, cholesterol and glucose. After wintering, there was an increase in creatinine, phosphorus and calcium. Knowing how much the period of optimal winter temperatures has shortened, and how this affects the state of carp at the physiological level, will provide an opportunity to develop recommendations for improving wintering technologies. Considering the dynamics of climate change, the research in this area is promising.
  PubDate: 2022-02-07
  DOI: 10.15421/022212
  Issue No: Vol. 13, No. 1 (2022)
Specificities of lipotoxicity of free fatty acids and cytokine profile in
patients with chronic diffuse liver diseases
- Authors: V. I. Didenko, I. A. Klenina, О. M. Tatarchuk, O. I. Hrabovska, O. P. Petishko
  Pages: 3 - 9
  Abstract: Non-alcoholic fatty liver disease is an important cause of global liver disease characterized by diffuse hepatocytes with hepatocellular ballooning, intrahepatic inflammation and progressive fibrosis. A relevant task is the study of the relationship between content of free fatty acids and serum cytokine profile in patients with chronic diffuse liver diseases. A total of 74 people with chronic diffuse liver diseases were examined, including 32 patients with non-alcoholic fatty liver disease, 22 patients with alcoholic liver disease, 20 patients with toxic hepatitis. Chromatographic examination of free fatty acids (FFA) in blood serum was carried out using a Chromatek-Crystal 5000 gas chromatography system. Patients with chronic diffuse liver diseases had a significant increase in the level of unsaturated free fatty acids (USFA) in cases of toxic hepatitis (by 2.92 times, P > 0.05) and a decrease in the level of saturated free fatty acids (SFA) in cases of non-alcoholic fatty liver disease (by 1.52 times, P > 0.05) compared with the control group; the balance between omega-6 and omega-3 PUFA significantly changed due to increase in linoleic acid in patients with alcoholic liver disease and toxic hepatitis (by 1.91 and 2.11 times, respectively) and arachidonic acid in patients with toxic hepatitis (by 1.78 times). The level of interleukin (IL)-6, IL-10, tumor necrosis factor alpha (TNF-α) were determined. In patients suffering chronic diffuse liver diseases there were multidirectional changes in the composition of free fatty acids of blood serum: a significant increase in the level of USFA, levels ІL-6 in toxic hepatitis; a decrease in the level of SFA, levels ІL-6 and TNF-α during non-alcoholic fatty liver disease; increased TNF-α production, ІL-6 during alcoholic liver disease compared with the control group. Significant change occurred in the balance between omega-6 and omega-3 PUFA due to increase in linoleic acid in cases of alcoholic liver disease and toxic hepatitis and arachidonic acid in cases of toxic hepatitis. The revealed correlations support the hypothesis that inflammation and lipotoxicity of FFA of blood serum contribute to the development and progression of structural changes in the liver. However, the pathomechanism of lipid metabolism and cytokine regulation with different etiological factors have their own characteristics, which should be taken into account when treating patients of these groups. Prospects for further research: these parameters may be used for serologic biomarkers of liver disease and development and implementation of the ratio between FFA and cytokines for the differential diagnosis of chronic diffuse liver disease in medical practice.
  PubDate: 2021-12-26
  DOI: 10.15421/022201
  Issue No: Vol. 13, No. 1 (2021)