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el-Hayah
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This is an Open Access Journal

Open Access journal
ISSN (Print) 2086-0064
Published by UIN Maulana Malik Ibrahim Malang

[13 journals]

Schleichera oleosa (Molk.)Oken Callus Induced BAP and 2,4 D In vitro
- Authors: Ruri Siti Resmisari, Azizatur Rahmah
  Pages: 118 - 123
  Abstract: Schleichera oleosa (Lour.) Oken is a plant that it can used as drug candidate. S. oleosa has several health benefits including as anti-cancer, anti-oxidant, and anti-microbial. One of technique for increasing the secondary metabolite in S. oleosa (Lour.) Oken is callus culture. This study is an experimental research using Factorial Completely Randomized Design (CRD). This study consist of 2 factors are Benzyl Amino Purin (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) with various concentrations. Based on the result of callus induction of S. oleosa (Lour.) Oken, it gives some different effect for the variable. The day of emerging callus and weight of callus give the optimum result at 2 mg/L 2,4-D + 0,5 mg/L BAP for 32 Day After Planting and 1,1458 grams. Besides, the percentage of callus formation at 1 mg/L 2,4-D + 0,5 mg/L BAP for 83,33%.
  PubDate: 2022-03-30
  DOI: 10.18860/elha.v8i4.15719
  Issue No: Vol. 8, No. 4 (2022)
The Ability of Entomopathogenic Fungus as Biocontrol of Periplaneta
americana
- Authors: Anggita Rahmi Hafsari, Ayu Nur Hidayah
  Pages: 124 - 129
  Abstract: Periplaneta americana (cockroach), are well-known pests that not only contaminate food with feces and germs that can cause food poisoning, but also transfer bacteria, fungus, and other dangerous microbes. Chemical pesticides are harmful to both the environment and individuals when used in excess. The natural balance has been disrupted, resulting in resistant pests, predatory threats, parasitic threats, fish, birds, and other animals. The objective of this research is to see the ability of an entomopathogenic fungus as a cockroach biocontrol agent. This research conducted with experimental research with ten treatments and three repetition. The variation are, K(-) only distilled water, K(+) synthetic pesticides, Botrytis sp., Mucors sp., Acremoniums sp., Rhizopus sp., Moniliella sp., Chaetomium sp., Nigospora sp., and Aspergillus sp. A one-way Completely Randomized Design was used to examine them (CRD). With a cell count of 2.35 x 108 CFU/ml, Rhizopus sp. was able to achieve a mortality rate of cockroach by 66.6 percent.
  PubDate: 2022-04-07
  DOI: 10.18860/elha.v8i4.15798
  Issue No: Vol. 8, No. 4 (2022)
Reptiles and Amphibian Diversity, Along with Potential Treat in Sumber
Nyolo, Malang Regency
- Authors: Berry Fakhry Hanifa, Muhammad Asmuni Hasyim, Bayu Agung Prahardika, Nurul Whika Agustin
  Pages: 130 - 135
  Abstract: Inventory of reptiles and amphibians was carried out in the Sumber Nyolo area, Karangploso District, Malang Regency, East Java. The sampling area is at an altitude of 600-650m above sea level. Pre-survey was conducted once. The survey was conducted from June-October 2020 using the visual encounter (VES) method. A total of 167 individuals from 17 species, 10 families have been recorded, 1 of which is in Near Threatened (NT) and Vulnerable (VU) status on the IUCN Redlist namely Ptyas korros and Huia masonii respectively, which H.masonii also endemic to Java Island. The diversity index (H’) shows a moderate value (2.099), while the dominance value (0,211) was lower than the evenness index (0.479). The species with the largest population was Chalchorona chalchonota (70 individuals), while Huia masonii, Ptyas korros Gekko gecko, and Xenochrophis sp. was the fewest species found (1 individual). Threats to this area include habitat destruction due to land conversion into tourism area and religious destination as well as rice fields in the downstream and settlement area
  PubDate: 2022-04-07
  DOI: 10.18860/elha.v8i4.15799
  Issue No: Vol. 8, No. 4 (2022)
Potential Analysis of Cheese Whey as an Alternative Media Growth for
Lactobacillus casei Group
- Authors: Nur Kusmiyati, Maria Massora, Septian Tri Wicaksono
  Pages: 136 - 146
  Abstract: Cheese processing industry throws a lot of whey into the environment that cause pollution. Whey cheese is known to contain lots of organic matter and lactose which is the main energy source for the Lactobacillus genus. This study aims to determine the effect of using cheese whey media on bacterial cell count, total lactic acid, and total sugar in the Lactobacillus casei group consisting of Lactobacillus casei, Lactobacillus paracasei, and Lactobacillus rhamnosus bacteria. The method used in this study was bacterial culture rejuvenated and propagated on MRSB media for 24 hours. As much as 1% of bacterial culture was inoculated into cheese whey medium and incubated at 37 C for 24 hours. Furthermore, observations were made on the number of bacterial cells, total lactic acid, and total sugar in the L. casei group. The results showed that L. paracasei had the highest number of cells (4.862 CFU/ml), total lactic acid (0.264 mg/ml), and total sugar (93.805 mg/ml) and was not significantly different from L. casei and L. rhamnosus. The cheese whey medium was not significantly different from the positive control (MRSB), however, there was a difference in the growth ability of each isolate on the positive control medium. Based on the research, it was shown that cheese whey media could be used as an alternative medium for the growth of the L. casei group.
  PubDate: 2022-04-08
  DOI: 10.18860/elha.v8i4.15800
  Issue No: Vol. 8, No. 4 (2022)
Effect of Blood Bags Storage Time on The Quality of HIV Pre Transfusion
Test Result: Preliminary Study in Blood Bank’s dr. Ben Mboi Ruteng
Manggarai District, East Nusa Tenggara Province
- Authors: Yani Suryani, Opik Taupiqurrohman, Yohanes Reynerius
  First page: 147
  Abstract: Research on the effect of storage time on blood quality has been conducted. The purpose of this study is to determine the quality changes caused by the length of storage. The test parameter is the change in blood to become HIV positive. The blood testing process is carried out by immune chromatography on blood that has been stored for 2 weeks, 3 weeks, 4 weeks and 5 weeks. Test results show changes in blood quality during storage. Time does not show a difference in changes in blood quality, however it needs to be aware, because the stored blood changes.
  PubDate: 2022-04-08
  DOI: 10.18860/elha.v8i4.15801
  Issue No: Vol. 8, No. 4 (2022)