Subjects -> BIOLOGY (Total: 3174 journals)
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BIOLOGY (1491 journals)                  1 2 3 4 5 6 7 8 | Last

Showing 1 - 200 of 1720 Journals sorted alphabetically
AAPS Journal     Hybrid Journal   (Followers: 24)
Abasyn Journal of Life Sciences     Open Access   (Followers: 2)
ACS Pharmacology & Translational Science     Hybrid Journal   (Followers: 3)
ACS Synthetic Biology     Hybrid Journal   (Followers: 30)
Acta Biologica Hungarica     Full-text available via subscription   (Followers: 5)
Acta Biologica Marisiensis     Open Access  
Acta Biologica Sibirica     Open Access   (Followers: 1)
Acta Biologica Turcica     Open Access   (Followers: 1)
Acta Biomaterialia     Hybrid Journal   (Followers: 31)
Acta Biotheoretica     Hybrid Journal   (Followers: 3)
Acta Chiropterologica     Full-text available via subscription   (Followers: 5)
acta ethologica     Hybrid Journal   (Followers: 5)
Acta Fytotechnica et Zootechnica     Open Access   (Followers: 2)
Acta Ichthyologica et Piscatoria     Open Access   (Followers: 4)
Acta Médica Costarricense     Open Access   (Followers: 2)
Acta Musei Silesiae, Scientiae Naturales     Open Access  
Acta Neurobiologiae Experimentalis     Open Access  
Acta Scientiae Biological Research     Open Access   (Followers: 1)
Acta Scientiarum. Biological Sciences     Open Access   (Followers: 2)
Acta Scientifica Naturalis     Open Access   (Followers: 2)
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis     Open Access   (Followers: 2)
Acta Universitatis Lodziensis : Folia Biologica et Oecologica     Open Access  
Actualidades Biológicas     Open Access   (Followers: 1)
Advanced Biology     Hybrid Journal   (Followers: 1)
Advanced Health Care Technologies     Open Access   (Followers: 12)
Advanced Journal of Graduate Research     Open Access   (Followers: 1)
Advanced Membranes     Open Access   (Followers: 4)
Advanced Quantum Technologies     Hybrid Journal   (Followers: 3)
Advances in Bioinformatics     Open Access   (Followers: 21)
Advances in Biological Regulation     Hybrid Journal   (Followers: 4)
Advances in Biology     Open Access   (Followers: 10)
Advances in Biomarker Sciences and Technology     Open Access   (Followers: 2)
Advances in Biosensors and Bioelectronics     Open Access   (Followers: 6)
Advances in Cell Biology/ Medical Journal of Cell Biology     Open Access   (Followers: 22)
Advances in Ecological Research     Full-text available via subscription   (Followers: 42)
Advances in Environmental Sciences - International Journal of the Bioflux Society     Open Access   (Followers: 14)
Advances in Enzyme Research     Open Access   (Followers: 10)
Advances in High Energy Physics     Open Access   (Followers: 23)
Advances in Human Biology     Open Access   (Followers: 6)
Advances in Life Science and Technology     Open Access   (Followers: 10)
Advances in Life Sciences     Open Access   (Followers: 5)
Advances in Marine Biology     Full-text available via subscription   (Followers: 23)
Advances in Tropical Biodiversity and Environmental Sciences     Open Access   (Followers: 4)
Advances in Virus Research     Full-text available via subscription   (Followers: 7)
Adversity and Resilience Science : Journal of Research and Practice     Hybrid Journal   (Followers: 3)
African Journal of Ecology     Hybrid Journal   (Followers: 17)
African Journal of Range & Forage Science     Hybrid Journal   (Followers: 12)
AFRREV STECH : An International Journal of Science and Technology     Open Access   (Followers: 3)
Ageing Research Reviews     Hybrid Journal   (Followers: 13)
Aggregate     Open Access   (Followers: 1)
Aging Cell     Open Access   (Followers: 23)
Agrokémia és Talajtan     Full-text available via subscription   (Followers: 2)
AJP Cell Physiology     Hybrid Journal   (Followers: 14)
AJP Endocrinology and Metabolism     Hybrid Journal   (Followers: 25)
AJP Lung Cellular and Molecular Physiology     Hybrid Journal   (Followers: 3)
Al-Kauniyah : Jurnal Biologi     Open Access  
Alasbimn Journal     Open Access   (Followers: 1)
Alces : A Journal Devoted to the Biology and Management of Moose     Open Access  
All Life     Open Access  
AMB Express     Open Access   (Followers: 1)
Ambix     Hybrid Journal   (Followers: 3)
American Journal of Agricultural and Biological Sciences     Open Access   (Followers: 6)
American Journal of Bioethics     Hybrid Journal   (Followers: 18)
American Journal of Human Biology     Hybrid Journal   (Followers: 15)
American Journal of Medical and Biological Research     Open Access   (Followers: 3)
American Journal of Plant Sciences     Open Access   (Followers: 19)
American Journal of Primatology     Hybrid Journal   (Followers: 15)
American Naturalist     Full-text available via subscription   (Followers: 78)
Amphibia-Reptilia     Hybrid Journal   (Followers: 4)
Anaerobe     Hybrid Journal   (Followers: 3)
Analytical Methods     Hybrid Journal   (Followers: 8)
Analytical Science Advances     Open Access   (Followers: 1)
Anatomical Science International     Hybrid Journal   (Followers: 3)
Animal Cells and Systems     Hybrid Journal   (Followers: 6)
Animal Microbiome     Open Access   (Followers: 1)
Animal Models and Experimental Medicine     Open Access  
Annales françaises d'Oto-rhino-laryngologie et de Pathologie Cervico-faciale     Full-text available via subscription   (Followers: 2)
Annales Henri Poincaré     Hybrid Journal   (Followers: 2)
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia     Open Access   (Followers: 1)
Annals of Applied Biology     Hybrid Journal   (Followers: 6)
Annals of Biomedical Engineering     Hybrid Journal   (Followers: 18)
Annals of Human Biology     Hybrid Journal   (Followers: 5)
Annals of Science and Technology     Open Access   (Followers: 2)
Annual Research & Review in Biology     Open Access  
Annual Review of Biomedical Engineering     Full-text available via subscription   (Followers: 18)
Annual Review of Biophysics     Full-text available via subscription   (Followers: 24)
Annual Review of Cancer Biology     Full-text available via subscription   (Followers: 3)
Annual Review of Cell and Developmental Biology     Full-text available via subscription   (Followers: 41)
Annual Review of Food Science and Technology     Full-text available via subscription   (Followers: 13)
Annual Review of Genomics and Human Genetics     Full-text available via subscription   (Followers: 27)
Annual Review of Phytopathology     Full-text available via subscription   (Followers: 11)
Anthropological Review     Open Access   (Followers: 27)
Antibiotics     Open Access   (Followers: 10)
Antioxidants     Open Access   (Followers: 4)
Antioxidants & Redox Signaling     Hybrid Journal   (Followers: 8)
Antonie van Leeuwenhoek     Hybrid Journal   (Followers: 3)
Anzeiger für Schädlingskunde     Hybrid Journal   (Followers: 1)
Apidologie     Hybrid Journal   (Followers: 4)
Apmis     Hybrid Journal   (Followers: 1)
APOPTOSIS     Hybrid Journal   (Followers: 8)
Applied Biology     Open Access   (Followers: 1)
Applied Bionics and Biomechanics     Open Access   (Followers: 4)
Applied Phycology     Open Access  
Applied Vegetation Science     Full-text available via subscription   (Followers: 9)
Aquaculture Environment Interactions     Open Access   (Followers: 6)
Aquaculture International     Hybrid Journal   (Followers: 24)
Aquaculture Reports     Open Access   (Followers: 3)
Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society     Open Access   (Followers: 8)
Aquatic Biology     Open Access   (Followers: 8)
Aquatic Ecology     Hybrid Journal   (Followers: 41)
Aquatic Ecosystem Health & Management     Hybrid Journal   (Followers: 15)
Aquatic Science and Technology     Open Access   (Followers: 2)
Aquatic Toxicology     Hybrid Journal   (Followers: 25)
Arabian Journal of Scientific Research / المجلة العربية للبحث العلمي     Open Access  
Archaea     Open Access   (Followers: 3)
Archiv für Molluskenkunde: International Journal of Malacology     Full-text available via subscription   (Followers: 1)
Archives of Biological Sciences     Open Access  
Archives of Microbiology     Hybrid Journal   (Followers: 9)
Archives of Natural History     Hybrid Journal   (Followers: 7)
Archives of Oral Biology     Hybrid Journal   (Followers: 2)
Archives of Virology     Hybrid Journal   (Followers: 6)
Archivum Immunologiae et Therapiae Experimentalis     Hybrid Journal   (Followers: 2)
Arctic     Open Access   (Followers: 1)
Arid Ecosystems     Hybrid Journal   (Followers: 2)
Arquivos do Museu Dinâmico Interdisciplinar     Open Access  
Arthropod Structure & Development     Hybrid Journal   (Followers: 2)
Arthropod Systematics & Phylogeny     Open Access   (Followers: 3)
Artificial DNA: PNA & XNA     Hybrid Journal   (Followers: 2)
Artificial Intelligence in the Life Sciences     Open Access  
Asian Bioethics Review     Full-text available via subscription   (Followers: 2)
Asian Journal of Biological Sciences     Open Access   (Followers: 2)
Asian Journal of Biology     Open Access  
Asian Journal of Biotechnology and Bioresource Technology     Open Access  
Asian Journal of Cell Biology     Open Access   (Followers: 4)
Asian Journal of Developmental Biology     Open Access   (Followers: 1)
Asian Journal of Medical and Biological Research     Open Access   (Followers: 3)
Asian Journal of Nematology     Open Access   (Followers: 4)
Asian Journal of Poultry Science     Open Access   (Followers: 3)
Atti della Accademia Peloritana dei Pericolanti - Classe di Scienze Medico-Biologiche     Open Access  
Australian Life Scientist     Full-text available via subscription   (Followers: 2)
Australian Mammalogy     Hybrid Journal   (Followers: 8)
Autophagy     Hybrid Journal   (Followers: 7)
Avian Biology Research     Hybrid Journal   (Followers: 4)
Avian Conservation and Ecology     Open Access   (Followers: 16)
Bacterial Empire     Open Access   (Followers: 1)
Bacteriology Journal     Open Access   (Followers: 2)
Bacteriophage     Full-text available via subscription   (Followers: 2)
Bangladesh Journal of Bioethics     Open Access  
Bangladesh Journal of Plant Taxonomy     Open Access  
Bangladesh Journal of Scientific Research     Open Access  
Berita Biologi     Open Access  
Between the Species     Open Access   (Followers: 2)
BIO Web of Conferences     Open Access  
Bio-Grafía. Escritos sobre la Biología y su enseñanza     Open Access  
Bio-Lectura     Open Access  
BIO-SITE : Biologi dan Sains Terapan     Open Access  
Bioactive Compounds in Health and Disease     Open Access  
Biocatalysis and Biotransformation     Hybrid Journal   (Followers: 5)
BioCentury Innovations     Full-text available via subscription   (Followers: 2)
Biochemistry and Cell Biology     Hybrid Journal   (Followers: 17)
Biochimie     Hybrid Journal   (Followers: 4)
BioControl     Hybrid Journal   (Followers: 2)
Biocontrol Science and Technology     Hybrid Journal   (Followers: 5)
Biodemography and Social Biology     Hybrid Journal  
BIODIK : Jurnal Ilmiah Pendidikan Biologi     Open Access  
BioDiscovery     Open Access   (Followers: 2)
Biodiversitas : Journal of Biological Diversity     Open Access   (Followers: 1)
Biodiversity : Research and Conservation     Open Access   (Followers: 28)
Biodiversity Data Journal     Open Access   (Followers: 7)
Biodiversity Informatics     Open Access   (Followers: 2)
Biodiversity Information Science and Standards     Open Access   (Followers: 1)
Bioeduscience     Open Access  
Bioeksperimen : Jurnal Penelitian Biologi     Open Access  
Bioelectrochemistry     Hybrid Journal   (Followers: 1)
Bioelectromagnetics     Hybrid Journal   (Followers: 1)
Bioenergy Research     Hybrid Journal   (Followers: 3)
Bioengineering and Bioscience     Open Access   (Followers: 1)
BioEssays     Hybrid Journal   (Followers: 10)
Bioethica     Open Access   (Followers: 1)
Bioethics     Hybrid Journal   (Followers: 21)
BioéthiqueOnline     Open Access   (Followers: 1)
Biogeographia : The Journal of Integrative Biogeography     Open Access   (Followers: 2)
Biogeosciences (BG)     Open Access   (Followers: 14)
Biogeosciences Discussions (BGD)     Open Access   (Followers: 4)
Bioinformatics     Hybrid Journal   (Followers: 219)
Bioinformatics Advances : Journal of the International Society for Computational Biology     Open Access   (Followers: 1)
Bioinformatics and Biology Insights     Open Access   (Followers: 13)
Biointerphases     Open Access   (Followers: 1)
Biojournal of Science and Technology     Open Access  
BioLink : Jurnal Biologi Lingkungan, Industri, Kesehatan     Open Access  
Biologia     Hybrid Journal   (Followers: 1)
Biologia Futura     Hybrid Journal  
Biologia on-line : Revista de divulgació de la Facultat de Biologia     Open Access  
Biological Bulletin     Partially Free   (Followers: 6)
Biological Control     Hybrid Journal   (Followers: 6)
Biological Invasions     Hybrid Journal   (Followers: 22)
Biological Journal of the Linnean Society     Hybrid Journal   (Followers: 19)
Biological Procedures Online     Open Access  
Biological Psychiatry     Hybrid Journal   (Followers: 52)
Biological Psychology     Hybrid Journal   (Followers: 5)

        1 2 3 4 5 6 7 8 | Last

Similar Journals
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Advances in Cell Biology/ Medical Journal of Cell Biology
Number of Followers: 22  

  This is an Open Access Journal Open Access journal
ISSN (Online) 2080-2218
Published by Sciendo Homepage  [370 journals]
  • Current application of exosomes in medicine

    • Abstract: Exosomes are a distinct type of extracellular vesicles that play a major role in intracellular transport and communication. Depending on the cell of origin, exosomes can contain diverse constituents of a cell, including DNA, RNA, lipids, metabolites, cytosolic and cell-surface proteins, playing important roles in a wide range of physiological and pathological processes. Due to these facts, they are subject of extensive research aiming at translating the knowledge into clinical approaches that are at the interface between nanomedicine and biopharmaceuticals.Their potential clinical use mostly revolves around the fields of diagnostics and drug delivery, especially important in treatment of cancer. The conventional and emerging methods of exosome isolation are either based on their physical properties (such as density and/or size) or their functions. However, the isolation approaches are still characterised by significant downsides, lacking standardisation, and ensuring purity. The review gives a critical overview on exosomes characteristics, isolation approaches and the potential that exosomes hold in developing new clinical approaches of modern medicine, highlighting the need for further research to fully grasp their potential and translate the knowledge into future therapeutic solutions.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Approaches for in vitro culture of granulosa cells and ovarian follicles

    • Abstract: The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have potential use in artificial reproductive technology (ART). Before ovulation, the follicle is formed by oocyte and cell populations known as granulosa cells (GCs). These cells build the internal and external mass of the follicular wall. Oocyte growth and proliferation of the surrounding cells depend on the gap junctions between the oocyte and the GCs. Maintenance of the optimal in vitro culture system allowing for preservation of follicle architecture and granulosa-oocyte interaction may be critical for success in vitro maturation of follicles. Recently many studies have focused on a culture of GCs, which have important functions related to steroidogenesis. Granulosa cells maintained in in vitro conditions exhibit stem cell properties making it important to consider in vitro culture (IVC) methods of the GC population.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Follicular renewal and stemness potency of follicular cells depended of
           telomerase activity and TERT expression – short review

    • Abstract: Several hypotheses have been proposed, relating to the potential genesis of follicular cells in the ovarian niche. Reports using mice as an experimental model have suggested that the ovaries may contain stem cells that are likely involved in the formation of new follicles in adult reproductive life. Over recent years, various types of ovarian cells have been identified and described to confirm or disprove the existence of ovarian adult stem cells. Most research is focused on granulosa cells (GCs), which are essential for follicular development and maturation of female germ cells (oocytes). GCs exhibit the features of stem cells, such as expression of stem cell markers: OCT-4, Sox-2, Nanog as well as certain markers of mesenchymal stem cells, including CD29, CD44, CD90, CD105, CD117, and CD166. Another discovery in favor of the potential stemness of GCs is their ability to transdifferentiate towards other cell lines and high telomerase (TERT) activity in dividing compartments of the follicle during its maturation.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Viability of bovine in vitro matured oocytes following ultra-rapid
           vitrification

    • Abstract: The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-rapid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P < 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Silica nanoparticles in targeted human cancer therapy

    • Abstract: One of the leading causes of death across the world is cancer. Despite massive attempts to develop efficient chemotherapy medications, there is still a significant toxicity and selectivity problem. We are looking for novel therapies and preventative strategies due to the toxicity of contemporary chemotherapy and cancer cell resistance to anticancer drugs. The structure and molecular characteristics of Na2SiO3 nanoparticles were investigated using density-functional theory calculations at the B3LYP/6-311G** level. The study looked at engineering qualities and several molecular recipes like HOMO, LUMO, and Egap in order to figure out how to arrange molecules as a powerful antioxidant, and hence the majority of the compounds are anticancer. We discovered that Na2SiO3 gel particles are responsible for antioxidant activity, implying that it can be employed as an antioxidant and anticancer for cancer prevention and treatment.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Changes of the zona pellucida patterns during oocyte maturation,
           fertilization and embryo development in mammals: mini-review

    • Abstract: The mammalian zona pellucida (ZP) is an extracellular matrix that surrounds immature and mature oocytes and early embryos until the stage of a blastocyst and its implantation. This mini-review summarizes basic information on the ZP and its morphologic and functional changes during in vitro oocyte maturation and fertilization and in vivo pre-implantation embryo development.
      PubDate: Sun, 10 Apr 2022 00:00:00 GMT
       
  • Aortocoronary conduits may show a different inflammatory response -
           comparative study at transcript level

    • Abstract: Coronary artery bypass grafting (CABG), together with percutaneous coronary intervention (PCI), are both still the most efficient procedures for myocardial revascularization to treat advanced coronary artery disease (CAD). Donor blood vessels used in CABG are usually the internal thoracic artery (ITA) and saphenous vein (SV). The importance of inflammation and inflammatory pathways in graft patency is well established. Nevertheless, not all molecular mechanisms underlying the inflammatory process appear to be clear. Employing the expressive microarray approach to analyze the transcriptome of both venous and arterial grafts, five GO BP terms has been selected: “cellular response to interferon-gamma”, “inflammatory response”, “interferon-gamma-mediated signaling pathway”, “response to interferon-gamma” and “positive regulation of inflammatory response”. This study aimed to evaluate potential molecular factors that could be characteristic markers for both SV and ITA conduits.Running title: Aortocoronary conduits may show a different inflammatory response
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • Cell-based approaches in drug development – a concise review

    • Abstract: In vitro models represent an alternative technique to in vivo or ex vivo studies in the drug development process. Cell-based assays are used to measure the level of proliferation and toxicity, as well as activation of signalling pathways and changes in morphology in cultivated cells. The studies conducted in vitro are aimed to estimate the newly synthesised drugs’ ability to permeate biological barriers and exert their therapeutic or cytotoxic effects. However, more than half of all studied drugs fail in the second or third phase of clinical trials due to a lack of confirmed efficacy. About a third of drugs fail because of safety issues, such as unacceptable levels of toxicity. To reduce attrition level in drug development, it is crucial to consider the implementation of translational phenotypic assays as well as to decipher various molecular mechanisms of action for new molecular entities. In this review, we summarise the existing cell-based methods most frequently used in the studies on drugs, taking into account their advantages and drawbacks.Running title: Cell-based approaches in drug development
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • New molecular markers involved in immune system homeostasis and
           hemopoietic organ development are differentially regulated during oocytes
           in vitro maturation

    • Abstract: The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation.Running title: Markers of hemopoietic organ development
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • New markers of human cumulus oophorus cells cultured in vitro –
           transcriptomic profile

    • Abstract: The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we used CCs from 12 patients aged 18-40 diagnosed with infertility. After harvesting cells on day 1, 7, 15 and 30 of culture, total RNA was isolated and transcriptomic analysis was performed. The DAVID software indicated the following GO BP terms: “cell junction organization”, “cell migration”, “cell morphogenesis involved in differentiation”, “cell morphogenesis” and “cell motility”. Of the genes belonging to all ontological groups, the most downregulated were: SLC7A8, DFNB31, COL1A1, CDC42SE1, TGFBR3, HMGB1, with the most upregulated genes being: ANXA3, KIAA1199, HTR2B, VCAM1, DKK1.While many studies focus on attempts to obtain fully competent oocytes, scientists still have difficulty attaining adequate results in vitro. Lack of adequate knowledge often results in low in vitro fertilization efficiency. Therefore, our research focuses on CCs cells, thanks to which the oocyte most likely acquires developmental competence. The main purpose of the study was to identify the potential molecular markers responsible for cell junction organization, migration, differentiation, morphogenesis and motility.Running title: New markers of human cumulus oophorus cells cultured in vitro
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • In search of markers useful for evaluation of graft patency - molecular
           analysis of ‘muscle system process’ for internal thoracic artery and
           saphenous vein conduits

    • Abstract: Coronary artery bypass graft (CABG) is the surgical method most commonly used to treat coronary artery disease (CAD). The vessels that are used in CABG are usually the internal thoracic artery (ITA) and the saphenous vein (SV). Transplant patency is one of the most important factors affecting transplant success. In this study, we used an expressive microarray method, approved by RT-qPCR, for transcriptome analysis of arterial and venous grafts. In the search for potential molecular factors, we analyzed gene ontologies of different expression based on the muscular system. Among interesting groups, we distinguished muscle cell proliferation, muscle contraction, muscle system process, regulation of smooth muscle cell proliferation and smooth muscle cell proliferation. The highest increase in gene expression was observed in: ACTN2, RBPMS2, NR4A3, KCNA5, while the smallest decrease in expression was shown by the P2RX1, KCNH2, DES and MYOT genes. Particularly noteworthy are the ACTN2 and NR4A3 genes, which can have a significant impact on vascular patency. ACTN2 is a gene that can affect the formation of atherosclerotic plaques, while NR4A3 occurs in 4 of the 5 ontological groups discussed and can affect the inflammatory process in the blood vessel. To summarize, the presented study provided valuable insight into the molecular aspects characterizing the vessels used in CABG, and in particular identified genes that may be the target for further studies on duct patency.Running title: CABG grafts’ molecular analysis of ‘muscle system process’
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • The processes of homeostasis, chemotaxis and organic and inorganic
           response are significantly up-regulated during short-term oral mucosal
           cells in vitro cultivation

    • Abstract: Mucous membranes appear in various parts of the whole body performing similar functions. However, they differ based on where the mucosa is located. It functions as a barrier in such systems as: respiratory, urogenital and digestive . In this study we will be focusing strictly on the oral mucosa. Keratinocytes and fibroblasts, which mainly form the structure of the oral mucosa, are subjected to numerous factors. Being one of the million parts that build the animal organism, they are involved in various processes. In this study, we will try to confirm that in the in vitro culture of oral mucosa cells, the expression of our selected genes undergoes significant changes which are tied to such processes as: homeostasis, chemotaxis and organic/inorganic response of the organism. For this study, 20 pubertal crossbred Landrace gilts were used. After slaughter, samples of buccal pouch mucosa were obtained and transported to the laboratory. The excised tissue was prepared and processed due to protocols. The final pellet was resuspended in supplemented DMEM. Once the cultures attained 70–80% confluency, they were passaged. Total RNA from each pooled sample was subjected to two rounds of sense cDNA amplification. The cDNA was processed on microarrays. Analysis of the scanned arrays was performed. The files were imported into downstream data analysis software. The DAVID analysis showed that differently expressed genes belongs to 56 Gene ontology groups. In this paper we focused on “cellular divalent inorganic cation homeostasis”, “chemical homeostasis”, “chemotaxis”, “homeostatic process” and “response to organic substance” GO BP terms. These sets of genes were subjected to hierarchical clusterization procedure. In summary, the data we collected showed primarily changes in gene expression that occurred in the thirty-day cell culture of oral mucosa tissue. We assume that indicated genes could be new gene markers for studied processes.Running title: Homeostasis in oral mucosa cells
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • Transcriptomic profile of genes encoding proteins responsible for
           regulation of cells differentiation and neurogenesis in vivo and in vitro
           – an oocyte model approach

    • Abstract: The growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs) - a population of granulosa cells - exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.Running title: Differentiation and neurogenesis in oocyte cells
      PubDate: Wed, 29 Apr 2020 00:00:00 GMT
       
  • The genes regulating maintenance of cellular protein location are
           differentially expressed in porcine epithelial oviductal cells during
           longterm in vitro cultivation

    • Abstract: The oviduct is a part of female reproductive tract that is essential for successful fertilization and early embryo development. It is lined with epithelium consisting of two types of cells: ciliated and secretory. The primary function of ciliated oviductal epithelial cells (OECs) is to support the transport of gametes and embryos through the ovary, whereas secretory OECs produce components of the oviductal fluid. Undoubtedly, the oviductal epithelium plays a major part in the early aspects of pregnancy development, from providing an optimal environment for gametes and embryos to supporting fertilization. Therefore, our aim was to gain a better insight into the genetic changes underlying function of these cells. We have harvested OECs from crossbred gilts (n=45), at the age of about nine months and which displayed two regular estrous cycles, and established long-term primary culture of porcine OECs. Microarray analysis was utilized to determine differentially expressed genes during day 1, 7, 15 and 30 of cultivation, with our results revealing54 differentially expressed genes belonging to three ontology groups: „maintenance of location”, „maintenance of protein location” and „maintenance of protein location in cell”. Since the biochemistry and morphology of epithelial cells may change during long term cultivation, we conclude that our results are a reflection of these changes and help to shed a light on porcine OECs properties in in vitro environment.Running title: Maintenance of cellular protein location in porcine epithelial oviductal cells
      PubDate: Sat, 12 Oct 2019 00:00:00 GMT
       
  • Genes encoding proteins regulating fatty acid metabolism and cellular
           response to lipids are differentially expressed in porcine luminal
           epithelium during long-term culture

    • Abstract: Among many factors, the epithelium lining the oviductal lumenis very important for the development of the oocyte and its subsequent fertilization. The oviductal epithelium is characterized by the presence of ciliary cells, supporting the movement of cumulus-oocyte complexes towards the uterus. By interacting with the semen, the epithelium of the fallopian tube makes the sperm acquire the ability to fertilize. So far, the exact molecular mechanisms of these changes have not been known. Hence, understanding the metabolism of oviduct epithelial cells and the level of expression of individual groups of genes seems to be a way to deepen the knowledge about the broadly understood reproduction.In our research, we decided to culture oviductal epithelial cells (OECs) in vitro for a long period of time. After 24h, 7, 15 and 30 days, the OECs were harvested, with their RNA isolated. Transcriptomic changes were analyzed using microarrays. The “cellular response to lipid” group was represented by the following genes: MUC1, CYP24A1, KLF4, IL24, SNAI2, CXCL10, PPARD, TNC, ABCA10, while the genes belonging to the “cellular lipid metabolic processes” were: LIPG, ARSK, ACADL, FADS3, P2RX7, ACSS2, PPARD, KITLG, SPTLC3, ERBB3, KLF4, CRABP2. Additionally, PPARD and ACADL were members of the “fatty acid beta-oxidation” ontology group. Our study describes genes that are not directly related to fertility processes. However, significant changes in their expression in in vitro cultured OECs may indicate their usefulness as markers of OECs’ physiological processes.Running title: Fatty acids changes in porcine oviductal epithelial cells in in vitro cultivation
      PubDate: Sat, 12 Oct 2019 00:00:00 GMT
       
  • Differential expression pattern of genes involved in oxygen metabolism in
           epithelial oviductal cells during primary in vitro culture

    • Abstract: Oxygen metabolism is crucial in establishing successful pregnancy, since excessive amount of reactive oxygen species (ROS) may exert deleterious effects on the developing embryo. There are several defense mechanisms against oxidative stress in the female reproductive tract, including production of antioxidant enzymes by oviductal epithelial cells (OECs). Undoubtedly, OECs play major part in female fertility and may also serve as an in vitro model of the oviduct. Therefore, the aim of this study was to investigate the expression of genes involved in oxygen metabolism. We have isolated OECs from oviducts of crossbred gilts (n=45) and maintained their in vitro culture for 30 days, collecting their RNA at days 1, 7, 15 and 30. The gene expression was determined with the use of Affymetrix® Porcine Gene 1.1 ST Array Strip. Our results revealed 166 differentially expressed genes belonging to four ontology groups: „cellular response to oxidative stress”, “cellular response to oxygen-containing compound”, “cellular response to oxygen levels” and “cellular response to reactive oxygen species”, most of which are also involved in other major processes in the organism. However, our findings provide a valuable insight into porcine reproductive biology and may be utilized in optimization of assisted reproduction techniques.Running title: Genes involved in oxygen metabolism in oviductal epithelial cells
      PubDate: Sat, 12 Oct 2019 00:00:00 GMT
       
  • Analysis of expression of genes responsible for regulation of cellular
           proliferation and migration – microarray approach based on porcine
           oocyte model

    • Abstract: The formation of mammalian oocytes begins in the ovary during fetal development. The proper development of oocytes requires close communication with surrounding somatic cells, the substances they emit allow proper maturation of oocytes. Somatic cumulus (CC) cells and oocytes form cumulus-oocyte (COC) complexes.In this study, the Affymetrix microarray analysis was used to investigate changes in gene expression occurring in oocytes before and after in vitro maturation (IVM). The aim of the study was to examine oocyte genes involved in two ontological groups, “regulation of cell migration” and “regulation of cell proliferation” discovered by the microarray method.We found a reduced expression of all 28 genes tested in the ontological groups: ID2, VEGFA, BTG2, CCND2, EDNRA, TGFBR3, GJA, LAMA2, RTN4, CDK6, IHH, MAGED1, INSR, CD9, PTGES, TXNIP, ITGB1, SMAD4, MAP3K1, NOTCH2 , IGFBP7, KLF10, KIT, TPM1, PLD1, BTG3, CD47 and MITF. We chose the most regulated genes down the IVM culture, and pointed out those belonging to two ontological groups.Increased expression of the described genes before IVM maturation may indicate the important role of these genes in the process of ovum maturation. After the maturation process, the proteins produced by them did not play such an important role. In summary, the study provides us with many genes that can serve as molecular markers of oocyte processes associated with in vitro maturation. This knowledge can be used for detailed studies on the regulation of oocyte maturation processes.Running title: Genes regulating cellular migration and proliferation in porcine oocytes
      PubDate: Sat, 12 Oct 2019 00:00:00 GMT
       
  • Genes regulating biochemical pathways of oxygen metabolism in porcine
           oviductal epithelial cells during long-term IVC

    • Abstract: Oxygen metabolism has an important role in the normal functioning of reproductive system, as well as the pathogenesis of female infertility. Oxidative stress seems to be responsible for the initiation or development of reproductive organ diseases, including polycystic ovary syndrome, endometriosis, preeclampsia, etc. Given the important role of maintaining balance between the production of ROS and antioxidant defence in the proper functioning of reproductive system, in the present study we aimed to analyse the expression of genes related to oxygen metabolism in porcine oviductal epithelial cells during long-term in vitro culture. The oviducts were collected from 45 crossbred gilts at the age of approximately nine months that displayed at least two regular oestrous cycles. The oviductal endothelial cells were isolated by enzymatic digestion to establish long-term primary cultures. Gene expression changes between 7, 15 and 30 daysof culturewere analysed with the use ofwhole transcriptome profiling by Affymetrix microarrays. The most of the “cellular response to oxidative stress” genes were upregulated. However, we did not observe any main trend in changes within the “cellular response to oxygen-containing compound” ontology group, where the gene expression levels were changed in various manner.Running title: Oxygen metabolism in porcine oviductal epithelial cells
      PubDate: Sat, 12 Oct 2019 00:00:00 GMT
       
 
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