JournalTOCs

Aquaculture International
Journal Prestige (SJR): 0.591

Citation Impact (citeScore): 2
Number of Followers: 25

Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1573-143X - ISSN (Online) 0967-6120
Published by Springer-Verlag

[2468 journals]

Development profile of digestive enzymes and intestinal nutrient
transporters during the early ontogeny of Himalayan snow trout,
Schizothorax richardsonii
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: This study characterized the ontogenic profile of key digestive enzymes and intestinal nutrient transporters in Schizothorax richardsonii larvae from 0 to 21 days post-hatch (dph; at 21.5 ± 0.2 °C) using biochemical and molecular approaches. The larvae exhibited an exclusive lecithotrophic phase until 8 dph, followed by an endogenous-exogenous transition phase until 15 dph (fed Artemia nauplii and larval diet), and complete exogenous feeding with larval diet during 15–21 dph. Regarding larval growth, weight gain exhibited an exponential trend, while the gain in total length followed a linear trend, increasing by 478% and 114%, respectively. The specific activity of trypsin and leucine aminopeptidase showed an increase at 8 dph and fluctuated thereafter during exogenous feeding, whereas total proteases and chymotrypsin activity was found to be high from 12 to 21 dph. Similarly, the expression of carboxypeptidase and peptide transporter pept1 showed a significant increase during 15–21 dph, as compared to 5–7 dph. Concerning lipid uptake, the mRNA levels of bile-activated lipase, hepatic lipase (lipc), and mttp showed an increasing trend during exogenous feeding, with peak expression at 21 dph. The amylase activity and the mRNA abundance of maltase (mgam), sodium-glucose cotransporter 1 (sglt1), and glucose transporter-2 (glut2) were found to increase significantly at 15–21 dph during exogenous feeding with formulated feed, as compared to the yolk absorption phase. Conversely, the abundance of apolipoprotein-b (apob) transcripts during the lecithotrophic phase indicates their potential role in utilizing yolk nutrients. The alkaline phosphatase activity showed a significant increase from 15 dph and peaked at 21 dph, indicating gut maturation and its role in aiding alkaline digestion. Overall, the development profile of digestive enzymes and intestinal nutrient transporters clearly indicates dietary stimulation and regulation, which is relevant for weaning and hatchery management of this agastric teleost.
  PubDate: 2025-04-16
The role of histological stains in aquaculture and fisheries development
technology
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Improper husbandry techniques, environmental pollution, fluctuating weather conditions, and shifting market dynamics pose significant challenges to aquaculture and fisheries production, making fish more susceptible to diseases. Understanding and characterizing the interventions involved in disease outbreaks are crucial, as they can lead to significant financial losses in the aquaculture and fisheries sectors. Histopathology plays a vital role in fish disease diagnosis, as it involves the microscopic examination of tissue abnormalities. This study aimed to evaluate the tissue preparation procedures for aquatic organisms and provide a historical review of tissue staining techniques. Over time, histopathological staining methods have advanced, leading to the development of simpler, more precise, and more efficient techniques. While newer genetic, non-culture, immunostaining, and other advanced staining methods have largely replaced traditional staining procedures, several older techniques remain in use. To enhance their effectiveness, modern histological stains are being modified and combined with other staining methods. Additionally, emerging fields like proteomics can complement traditional approaches, such as pathogen identification and histological examination, to achieve more accurate disease diagnoses in aquaculture and fisheries.
  PubDate: 2025-04-16
Interactive effects of temperature and dietary protein to energy ratios on
the growth and physiology of marron (Cherax cainii, Austin & Ryan 2002)
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Determining the optimum dietary protein to energy (P:E) ratios in aquaculture species is vital to allow for protein-sparing and faster growth rates, which are highly influenced by temperature, while avoiding the adverse physiological problems associated with high dietary lipids or poor nutrition. A laboratory feeding trial was conducted to investigate the effects of three diets of varied P:E ratios and two different temperatures (18 °C, 22 °C) on the growth, condition, survival and physiology of marron (Cherax cainii Austin and Ryan, 2002). The marron were fed three isonitrogenous diets: LL — Low lipid (6%), ML — moderate lipid (10%) and HL — high lipid (14%), with P:E ratios of 19.1, 17.4 and 16.2 g MJ−1 respectively for 120 days. At the end of the feeding trial, growth of marron increased at 22 °C in all dietary treatments. Marron cultured at 18 °C with LL had a lower Total Haemocyte Count, and there was no effect of temperature or diet on the organosomatic indices, condition or survival rate. Total meat yield was higher at 18 °C than at 22 °C overall. HL at 18 °C resulted in lower growth, lower orbital carapace length increase and a high feed conversion ratio, though there was no effect of dietary lipid content on growth at 22 °C. The temperature had an overarching effect on marron growth, and had a greater influence on marron fed a high lipid diet, where there was a greater difference in weight gain of marron between temperatures with the high lipid diet. The present study concludes that a diet with a P:E ratio of 17.4–19.1 g MJ−1, a lipid level of 6–10% and an energy content of 15–16 MJ kg−1 was optimal for marron under the tested experimental conditions.
  PubDate: 2025-04-15
Aquaculture mathematical modelling review
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: World aquaculture has demonstrated stable growth in production volumes for the last three decades. The competitiveness of the aquaculture market is growing steadily and the amount of data that fish-farmers have to manage is rapidly increasing. Mathematical modeling is the key factor in improving the fish farming industry and switching from experience-based to knowledge-based precision aquaculture. In this context, mathematical models are crucial tools for sustainable development. This paper presents a review of the literature on mathematical modeling in aquaculture. Using the scientific web search engine Google Scholar, we discovered 154 characteristic research papers considering mathematical models. We perform manual summarizing and classification of the papers by aquaculture problem, scale of the models, and methods used, and develop a classification. In total, we discovered mathematical models related to twenty different aquaculture problems and four scales, from cage/pool-wide to region-wide. We also describe the mathematical methods most commonly used and demonstrate examples of which specific mathematical methods are applied to solve aquaculture problems.
  PubDate: 2025-04-15
Insights into the utilization of Ulva prolifera hydrolysate to enhance
substance production of Isochrysis galbana: a win–win strategy
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Isochrysis galbana is considered a promising feed for aquaculture, but high cultivation cost well as low substance yield and quality limit its application prospects. This study explored the feasibility and regulating mechanism of green tide waste Ulva prolifera for I. galbana substance production. U. prolifera hydrolysate (UPH) was prepared as an alternative substrate from 5 to 50% concentrations to culture I. galbana. Compared to the control f/2 medium, the biomass production of I. galbana added with 40% UPH reached 318.75 mg/L, which was the most effective up to 1.24-fold increase. Meanwhile, the highest pigments, lipid, protein and carbohydrate productions were observed in the 40% UPH substrate, which were around 1.17 to 1.70 times higher than that of the control, respectively. The above parameters were the lowest with the addition of 5% UPH, except for the lipid content, which was slightly higher than control. The photosynthetic activity showed that 5% UPH blocked four electron transfer sites of I. galbana, including from Quinone A (QA) to QB, from plastoquinone (PQ) to photosystem I (PSI), the oxygen evolving complex (OEC) activity on the donor side and the acceptor pool on the PSI. However, 40% UPH enhanced the electron transfer beyond QA, from PQ to PSI and the acceptor pool of PSI. The photosynthetic parameters φPo, φEO, PICS and PIABS, which had strong correlation with the biomass and substance accumulation of I. galbana, were found to be enhanced by 40% UPH. Changes in electron transfer regulated the photosynthetic activity and metabolism of I. galbana. Encouragingly, the production cost of cultivating I. galbana with UPH prepared from green tide waste was almost negligible compared with the f/2 medium (US$ 15.6). Therefore, the study provides a win–win strategy for obtaining cost-effective energy production as well as the resource utilization of green tide waste. Graphical Abstract
  PubDate: 2025-04-14
DeepSeek-Based Multi-dimensional Augmentation of Short and Highly
Domain-Specific Textual Inquires for Aquaculture Question-Answering
Framework
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: High-quality data are essential for accurate and timely decision-making in disease prevention and control within aquaculture question-answering (QA) framework. However, textual data reflecting conversational question–answer exchange between fishery farmers and domain experts remain scarce, hindering the progress in training and building such systems. To address this gap, we introduce a multi-dimensionalaugmentationapproach leveraging DeepSeek to generate high-quality augmented data tailored to aquaculture, concentrating on the questions side. Our method aims to provide high quality synthesized data to better train aquaculture question-answering frameworks to gain comprehensive understanding of key information from short, free-form and conversational inquiries and deduct the intention of the questions. We employ a multi-task BERT framework to assess the reliability and diversity of these augmented samples, ensuring they preserve core semantics while expanding domain-specific data availability. We benchmark our approach against ChatGPT o1 and our experimental results demonstrate that DeepSeek achieves better performance. Specifically, for domain specific key information (aka entity) recognition, it attains an accuracy of 92.08%, precision of 92.3%, recall of 92.05%, and an F1 score of 91.78%; for intent classification, the model reaches 91.67% accuracy, 93.48% precision, 91.67% recall, and 89.68% F1 score. Notably, DeepSeek surpasses ChatGPT o1 in intent classification and remains competitive in key entity recognition. Furthermore, the augmented samples exhibit robust domain reliability (Cosine similarity
  PubDate: 2025-04-14
TDA-GLM: text data augmentation for aquaculture disease prevention and
control via a small model-guided ChatGLM
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Disease prevention and control is crucial for the healthy development of the aquaculture industry, and high-quality data are the foundation for intelligent disease management. However, textual data in this field are scarce and variable in quality. The direct use of large language models (LLMs) for data augmentation often results in poor-quality outputs. In this paper, we propose a small-sample data augmentation framework called TDA-GLM. This framework employs a strategy that combines a high-quality corpus, pretrained large model, and efficient prompts, which improves data augmentation by integrating high-quality corpora and refining prompt techniques. To address the poor adaptability of LLMs in aquaculture disease prevention and control, which leads to low-quality data augmentation, we introduce a supervised learning model. This model guides the understanding of domain knowledge and optimizes data augmentation tasks within the LLM, enabling the generation of data that are both highly similar to the original professional material and rich in content. Additionally, we design a noise removal module that filters out noise by analyzing the consistency between the augmented data and the data augmentation target, thereby increasing the overall quality of the augmented data. To verify the domain reliability of our data augmentation approach, we conducted a few-shot learning classification task experiment. The results demonstrated significant improvements by our model over existing advanced text data augmentation techniques, with the key performance indicators Acc, P, R, and F1 reaching 94.86%, 95.55%, 94.86%, and 94.62%, respectively. Furthermore, in the experiments assessing the quality of the augmented samples, the similarity and enrichment degrees reached 94.95% and 64.41%, respectively. These results indicate that our augmented samples are of very high quality, ensuring that the core semantics of the original data are preserved while increasing data diversity through appropriate variations.
  PubDate: 2025-04-14
Effects of stocking density on growth, behavior, digestion, and gut health
of the sea cucumber Apostichopus japonicus: new insights into land-based
aquaculture
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: The increasing demand stimulates the rapid development of sea cucumber aquaculture industry in China. High stocking density is commonly used in the land-based aquaculture, but greatly affects the growth rate of sea cucumbers. In the present study, two different stocking densities were set to investigate the effects on growth, fitness-related behaviors, digestion, and gut health of the sea cucumber Apostichopus japonicus. We found that the terminal wet weight and weight gain rate significantly reduced in sea cucumbers at high stocking density. Feeding behavior significantly decreased in those at high stocking density, while crawling behavior was not affected. Protease activity was significantly inhibited in sea cucumbers at high stocking density. This may be an important reason for the reduced feeding behavior. Intestinal morphology showed that intestinal diameter, folds height, and enterocyte height significantly reduced in sea cucumbers at high stocking density. Degenerated intestinal structure is closely related to inhibited protease activity. Furthermore, the abundance of Proteobacteria increased, but the abundance of Bacteroidetes reduced in the gut of sea cucumbers at high stocking density. This result suggests that gut health was affected in sea cucumbers at high stocking density. In conclusion, reduced feeding behavior, degradation of intestinal structure, and changed intestinal microbial composition are important reasons affecting the growth of sea cucumbers. This finding provides valuable information for the high-density land-based aquaculture of A. japonicus.
  PubDate: 2025-04-14
A hybrid enhanced optimization architecture-based model for long-term
water temperature prediction in aquaculture
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Addressing issues such as inadequate prediction accuracy, significant noise interference, and difficulties in capturing long-term dependencies in aquaculture environment water temperature (WT) forecasting, this paper proposes a long-term prediction model based on a hybrid enhanced optimization architecture, namely the HEOA-BiTCN-MLSTM model. This model utilizes the human evolutionary optimization algorithm (HEOA) to optimize model parameters, thereby improving prediction accuracy. It employs a bidirectional temporal convolutional network (BiTCN) to deeply extract latent features from data, enhance noise resistance, and capture long-term trends and complex nonlinear variations in WT data. Finally, it integrates a multi-head long short-term memory (MLSTM) network, which combines the long short-term memory (LSTM) network with the multi-head self-attention mechanism (MHSA), to improve the model’s ability to capture long-term dependencies and model global information when processing long sequences. This model significantly improves WT prediction performance through the synergistic effects of optimization, hybridization, and enhancement while effectively addressing noise interference and data irregularity in complex environments. Experimental results demonstrate that this model significantly outperforms traditional benchmark models such as the gated recurrent unit (GRU), backpropagation neural network (BPNN), and recurrent neural network (RNN) in long-term WT prediction. Specifically, when accurately predicting WT 4 h ahead, the model achieves R2, MSE, MAE, and wMAPE values of 0.912, 0.352, 0.191, and 1.390%, respectively. This research provides robust support for intelligent monitoring and regulation in aquaculture environments.
  PubDate: 2025-04-12
Trait segregation and growth characteristics of the F2 generation hybrids
between Crassostrea dianbaiensis and C. iredalei
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Crassostrea dianbaiensis, a recently identified species with significant commercial potential, has been successfully hybridized with Crassostrea iredalei, demonstrating that fertilization can be bidirectional. Notably, only the DI hybrid lineage (C. dianbaiensis♀ × C. iredalei♂) achieves maturity. These DI hybrids display remarkable growth vigor and survival advantages over their parental strains. To further investigate the trait segregation patterns in the F2 generation and the stable inheritance of heterosis, an intraspecific-crossing experiment was conducted with mature DI individuals, and place the juvenile shellfish in natural sea areas and artificial ponds for comparative aquaculture. The study involved crossing within C. dianbaiensis, C. iredalei, and mature DI individuals. The results indicated that F2 hybrids could fertilize successfully and develop normally, but exhibited signs of outbreeding depression during the planktonic larval stage compared to the grandparental species. Genetic analysis using ITS1 markers classified F2 individuals into three distinct categories: F2-I, F2-D, and F2-H. Water temperature fluctuations during the growth phase in Oushi’s ponds were more extreme than those observed in the Sanbaimen sea. On day 360, the ratio of F2-I, F2-D, and F2-H in Oushi’s ponds was approximately 1:1:4, contrasting with a 1:1:2 ratio in the Sanbaimen sea. This indicates that F2-H has a higher survival rate in Oushi’s ponds with drastic temperature changes. In addition, nearly all F2-D individuals showed phenotypic traits surpassing the F2 average. Histological examination of gonads indicated that all three F2 types were capable of gamete production, albeit with delayed gonadal development relative to age-matched grandparental species. In conclusion, both F2-H and F2-D exhibited heat tolerance and enhanced growth rates, respectively, suggesting their potential as new foundational breeding stocks for targeted development of strains adapted to large-scale aquaculture in southern China.
  PubDate: 2025-04-11
Successful high replacement of fishmeal in rainbow trout feed by consortia
of aerobic heterotrophic bacteria results in equal growth, health, and
muscle composition
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: The expanding aquaculture industry faces challenges due to limited fishmeal production, some of which is unsustainably sourced. Developing alternative protein sources for aquafeed is crucial to enhance the sector’s long-term sustainability. Microbial protein, and more particularly single-cell protein as dried microbial biomass, shows promise due to its relatively low environmental footprint. Open consortia of aerobic heterotrophic bacteria (AHB) enable an innovative and cost-efficient manner to upgrade resources from feed-grade wastewater, promoting a more circular and sustainable system. High-Rate Conventional Activated Sludge (HiCAS) and High-Rate Contact Stabilization (HiCS) are suitable compact AHB production processes. However, to the authors knowledge, no studies have explored AHB produced in HiCAS or HiCS systems as a fishmeal replacement. This pioneering research aimed to assess the impact of substituting fishmeal with HiCAS and HiCS biomass on the growth performance, health, and muscle quality of juvenile rainbow trout. The 7-week feeding trial utilized AHB biomass grown on brewery wastewater, with isonitrogenous, isolipidic, and isocaloric diets. Fishmeal substitution levels were 25%, 50%, and 75% for both HiCAS and HiCS AHB biomass. Results indicated that AHB diets performed similarly to the control (p> 0.05) in weight gain, specific growth rate, and feed conversion ratio. Fitness parameters, including standard metabolic rate, hepatosomatic index, viscerosomatic index, condition factor, and muscle quality, showed comparable observations between AHB diets and control. Overall, these findings suggest that both HiCAS and HiCS biomass can replace up to 75% of fishmeal protein content with similar effects on fish growth, health, and fillet quality. This research underscores the potential of AHB biomass as a sustainable alternative protein source for aquafeed.
  PubDate: 2025-04-11
Gene expression responses unveil the negative effects of Olive Mill
Wastewater on Gammarus komareki (Amphipoda: Gammaridae)
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Olive Mill Wastewater (OMW) is a major organic pollutant, particularly in Mediterranean countries with intensive olive oil production. In this study, the toxic effects of OMW were tested on a freshwater macroinvertebrate, Gammarus komareki. Animals were exposed to different concentrations of 1/5, 1/10, and 1/20 of the previously calculated 72-h LC50 (LC50 = 3.65%) and was applied as high (0.73%), medium (0.365%) and low (0.1825%) levels, respectively for 20 days. At exposure, female and male individuals from each group were sampled separately. Samples were analyzed for the expression levels of antioxidant defences (cat, gst, se-gpx, and mn-sod), reproduction (vitellogenin-like protein, vtg), respiration (hemocyanin, hc), antitoxic defences (heat shock protein 70, hsp70), and molting (molt-inhibiting hormone, mih) related genes. Results revealed that the expression levels of vtg gene in females exposed to low doses were increased significantly compared to other groups; however, its levels were decreased in group exposed to high doses compared to the control and other groups. The mRNA expression levels of mn-sod and se-gpx genes of males and female individuals exposed to low OMW doses increased significantly compared to other doses. The mRNA expression levels of gst gene increased in males and females exposed to moderate doses and only in males exposed to low doses compared to other groups. The mRNA expression levels of cat gene of females were similar in all groups, while their levels were higher in males exposed to OMW than in the control group. The expression values of hsp70 gene were significantly decreased in females and all males exposed to high doses of OMW. On the other hand, the mih gene expression in females was increased at low doses of OMW, while decreased in the groups exposed to high doses compared to the control group. In male individuals, the mih expression levels significantly increased in all experimental groups. The expression of the hc gene was decreased in male and female individuals exposed to all doses of OMW compared to the control group. To put it briefly, the great variations in the gene expression levels in groups exposed to OMW underlines the toxic effects of this effluent and its negative impacts on the aquatic organisms.
  PubDate: 2025-04-10
Dietary zinc requirement of Catla (Catla catla) juveniles
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Zinc (Zn) is an essential inorganic micronutrient in fish, as it plays important roles in many biological functions. This study was performed to evaluate the dietary Zn requirements of Catla catla and its impacts on growth, proximate composition, nutrient utilization and antioxidant enzyme activities. Six experimental diets were prepared from the basal diet supplemented with 0, 20, 40, 60, 80 and 100 mg/kg Zn from ZnSO4, and the Zn contents were 2.15, 22.11, 42.14, 62.06, 81.89 and 101.63 mg/kg, respectively. Each experimental diet was fed in triplicate for 60 days, and each replicate contained 15 fish (initial weight 12.581 ± 0.1293 g). At the end of the trial, increasing the dietary Zn concentration to 62.06 mg/kg significantly (p
  PubDate: 2025-04-10
Mitigating effects of seaweed on gene expression and histological damage
in Nile tilapia under acute ammonia stress and bacterial infection
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Ammonia exposure can be considered more stressful for aquatic animals when it is concurrent with bacterial infection. In this regard, the study was planned to evaluate ammonia stress, the Aeromonas hydrophila infection, and their synergic effect on Nile tilapia to understand this interaction and the inclusion of seaweed extract (ASE), a natural agent for rearing water to reduce these negative influences. Seven groups of fish with the same initial weight were allocated as the control group: T0 (free of ammonia, A. hydrophila, and ASE), fish exposed to ammonia (2.5 mg/L), A. hydrophila stress, concurrent ammonia, and A. hydrophila stress without ASE (T1–T3) while groups (T4–T6) treated with ASE respectively. Data illuminated that an adverse effect on haemato-biochemical and immunological indices was seen in fish exposed to ammonia and A. hydrophila stress separately or together, and these indices improved after being treated with ASE, particularly in T4 and T5 fish groups. Likewise, antioxidant activity significantly decreased (SOD and CAT) along with increased MDA levels in fish subjected to the stress of ammonia and A. hydrophila separately or together without ASE while improved in these indices after being treated with ASE, especially in T4 and T5 fish groups. The expressions of TNF-α, HSP70, LZM, COX-2, C3, IL-1β, and IL-8 genes were downregulated in ASE-treated fish and upregulated in fish subjected to ammonia and A. hydrophila infection separately or together. Conversely, SOD CAT and IL-10 gene expression were elevated in ASE-treated fish (T3–T6) and declined in fish subjected to ammonia and A. hydrophila infection separately or together (T1-T3). The histopathology analysis showed that fish subjected to ammonia and bacterial infection displayed inflammatory and irregular characteristics, while fish treated with ASE induced anti-inflammation effects. Hence, the study concluded that ammonia increases the infection of A. hydrophila, and treated with AES protects Nile tilapia from these stresses.
  PubDate: 2025-04-10
Comparison and mechanism analysis of fatty acid differences between
backcross F2 derived from blunt snout bream (Megalobrama amblycephala,♀)
× topmouth culter (Culter alburnus,♂) and its closely related species
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: High-quality fatty acids are essential indicators of the nutritional value of aquatic fish. This study investigates the differences in muscle fatty acid composition and their nutritional implications by integrating physiological and transcriptomic analyses to elucidate the underlying regulatory mechanisms in the New Hybrid Bream (BBTB) compared with four closely related species: Megalobrama hoffmanni (MH), Megalobrama terminalis (MT), Megalobrama amblycephala (MA), and Culter alburnus (CA). A total of 21 fatty acids were identified in muscle tissue through gas chromatography, including 7 saturated fatty acids (SFAs) and 14 unsaturated fatty acids (UFAs). Among all species, palmitic acid (C16:0), oleic acid (C18:1n- 9), and linoleic acid (C18:2n- 6c) were the most abundant, accounting for 71.44 to 77.74% of the total fatty acids. Notably, BBTB exhibited a higher proportion of n- 6 polyunsaturated fatty acids (PUFAs), particularly linoleic acid, gamma-linolenic acid, arachidonic acid (C20:4n- 6), and eicosadienoic acid (C20:2). Subsequently, the expressions of fatty acid synthesis, degradation, and transporting related genes were examined to explore the mechanism of elevated PUFA in BBTB. The result exhibited that the expression of fatty acid synthesis key genes fasn, fads2, fads6, acacb, srebp1, acss2, and dgat2 genes of BBTB was significantly higher than that of other groups. Additionally, the expressions of pparα, pparδ, and pparγ which are critical regulators in the PPAR signaling pathway were significantly elevated in BBTB, suggesting their involvement in fatty acid synthesis, degradation, and oxidation. Further, transcriptomic analysis of BBTB liver further confirmed the gene expression results. Transcriptomic analysis of BBTB liver identified 125 differentially expressed genes (DEGs), with a subset associated with fatty acid metabolism, including pathways related to fatty acid degradation, PPAR signaling, and polyunsaturated fatty acid biosynthesis. KEGG enrichment analysis demonstrated significant enrichment of DEGs in lipid metabolism pathways, and COG and GO annotation further verified a high proportion of DEGs functioned in lipid transport, post-translational modifications, protein transformation, and chaperone activity. These findings provide valuable insights into the different values of BBTB and other examined group fishes and regulatory mechanisms governing lipid metabolism in BBTB, offering a foundation for improving the nutritional value of fish in aquaculture.
  PubDate: 2025-04-09
Effects of long-term photoperiod on growth, gonad yield and health status
of the sea urchin Strongylocentrotus intermedius
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: Enhancing growth, gonad yield, and health status in cultured sea urchins is crucial. This study examined the growth, gonadal development, and immune response of the sea urchin Strongylocentrotus intermedius subjected to five distinct photoperiod regimes (light–dark ratio of 0L: 24D, 8L: 16D, 12L: 12D, 16L: 8D, and 24L: 0D) for a duration of 13 months. The findings indicated that sea urchins in the 0L treatment achieved the largest body weight overall, however, they attained the maximum specific growth rate (SGR) only during the initial 0 to 3 months stage. In the latter phases, sea urchins subjected to the 12L or 16L treatment attained the highest SGR. This outcome indicated a heightened requirement for light as body size increases. The photoperiod regime significantly influenced the gonad yield of the experimental sea urchins (P < 0.05), with individuals in the 16L treatment attaining the greatest gonad weight and the highest gonad index by the conclusion of the experiment. The photoperiod regime did not significantly influence the gonadal coloration of the experimental sea urchins. Histological analysis indicated that the proportion of mature individuals decreases with the reduction of daily light duration. The 0L and 8L photoperiods may regulate the excessive gonadal development in young S. intermedius. At the end of the experiment, no significant change in the phagocytic rate or phagocytic index was seen between the treatments. Finally, long-term photoperiods did not influence the survival rate or immunity of S. intermedius. Our findings indicated that in land-based aquaculture systems, the sea urchin S. intermedius may be cultivated in dark or under reduced daily light to achieve rapid growth, followed by cultivation in a 16L photoperiod to enhance gonad output at harvest.
  PubDate: 2025-04-05
In vivo and in silico preliminary evaluation of the cyanobacterial
peptides portoamides A and B against the white spot syndrome virus in
freshwater crabs (Paratelphusa hydrodomous)
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: White spot syndrome, caused by the white spot syndrome virus (WSSV), is a devastating viral disease responsible for significant economic losses in the shrimp farming industry. In the search for natural therapeutic alternatives against WSSV, this study explored the antiviral potential of portoamides, natural peptides produced by the cyanobacterium Phormidium sp. LEGE 05292. Given that the structural proteins of the viral envelope, specifically VP28, VP26, and VP24, are the primary mediators of host cell attachment, they serve as promising targets for antiviral drug development. Our approach combined in vivo post-infection histopathological analysis with in silico molecular docking to assess the antiviral efficacy of portoamides. In the in vivo study, crabs were injected with portoamides alongside WSSV and monitored for 30 days post-infection. The antiviral activity of portoamides was evaluated through survival rates and histopathological observations. The results revealed that crabs treated with portoamides showed improved survival and reduced signs of viral infection compared to the control group. In parallel, in silico molecular docking analysis was conducted to assess the binding affinity between portoamides and the viral envelope proteins VP28, VP26, and VP24. The docking results demonstrated that these proteins exhibited the highest binding energies with portoamides, indicating a strong interaction that could potentially inhibit viral attachment and replication. Our findings suggest that portoamides effectively inhibit WSSV replication by interacting with the viral envelope proteins, thereby preventing the virus from establishing infection in crabs. Moreover, it is hypothesized that portoamides may stimulate the immune system in crabs, further enhancing resistance to WSSV infection. However, additional studies are needed to fully understand the immunomodulatory mechanisms involved. These preliminary results highlight the potential of portoamides as natural antiviral agents for combating WSSV in aquaculture settings, paving the way for future research on their application in disease management strategies.
  PubDate: 2025-04-05
Feed enriched by autochthonous probiotic bacteria and its effect on growth
and immune response under different feeding regimes and immune response
after Aeromonas salmonicida infection in rainbow trout (Oncorhynchus
mykiss)
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: This study examined the impact of a newly developed probiotic feed using the autochthonous probiotic strain Lactobacillus plantarum R2 Biocenol™ on the immune response of healthy rainbow trout, particularly after infection with Aeromonas salmonicida subsp. salmonicida. A probiotic feed was tested using different application strategies—continuous and cyclic feeding—to assess its influence on immune function. In the first phase, a non-infectious experiment was conducted to compare these feeding approaches and their effects on immune-related gene expression over time. The second phase involved an infection trial in which fish previously supplemented with probiotics were exposed to a bacterial pathogen to evaluate potential protective effects. Results indicated that while continuous probiotic feeding did not significantly alter immune function, cyclic feeding was associated with a temporary boost in immune response, as evidenced by changes in the expression of key immune-related genes (cd8, igm, il-8, tnf-α). Furthermore, the body length and weight improved in fish fed with probiotics increased over the study period. One week post-infection, pro-inflammatory cytokine expressions (il-1, il-8, tnf-α) were reduced in the probiotic group, with a similar trend observed for other pro-inflammatory molecules. The second infectious sampling indicated increased expression of cd4 and cd8 genes, suggesting heightened acquired immunity in the probiotic group. These findings suggest that probiotics can influence immune function and may contribute to improved disease resistance in aquaculture.
  PubDate: 2025-04-04
Correction to: The effect of aqueous application of probiotics on growth,
heavy metal accumulation, blood biochemistry, and histological alterations
of Dicentrarcus labrax
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  PubDate: 2025-04-04
Effects of temperature on growth performance, gonad development, immunity,
and antioxidant response of hybrid red tilapia (Oreochromis niloticus
♂ × Oreochromis aureus ♀)
- Free pre-print version: Loading...
  Rate this result: What is this?
  
  Please help us test our new pre-print finding feature by giving the pre-print link a rating.
  A 5 star rating indicates the linked pre-print has the exact same content as the published article.
  
  Abstract: The alteration of water temperature influences fishery productivity. The aim of the present study was to investigate the impact of water temperature on the growth performance, gonad development, immunity, and antioxidant functions of hybrid red tilapia (Oreochromis niloticus × Oreochromis aureus). The experimental fish were reared at four different temperatures (22, 25, 28, and 31 ℃). The fish reared at 28 ℃ had the highest weight gain and the highest specific growth rate, but a lower feed conversion ratio. Increasing the water temperature to 28 ℃ promotes the development of the gonads of this fish; however, lower temperatures (22 ℃) can inhibit its development. The fish reared at 28 ℃ had the highest gonad somatic index (P
  PubDate: 2025-04-04

HOME > Browse the 73 Subjects covered by JournalTOCs
Subject	Total Journals