JournalTOCs

Journal of Histochemistry and Cytochemistry
Journal Prestige (SJR): 1.344

Citation Impact (citeScore): 3
Number of Followers: 7

Hybrid journal (It can contain Open Access articles)
ISSN (Print) 0022-1554 - ISSN (Online) 1551-5044
Published by Sage Publications

[1174 journals]

Morphology of Schwann Cell Processes Supports Renal Sympathetic Nerve
Terminals With Local Distribution of Adrenoceptors
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  Authors: Seishi Maeda, Yusuke Minato, Sachi Kuwahara-Otani, Hiroki Yamanaka, Mitsuyo Maeda, Yosky Kataoka, Hideshi Yagi
  Abstract: Journal of Histochemistry & Cytochemistry, Ahead of Print.
  Nerves in the renal parenchyma comprise sympathetic nerves that act on renal arteries and tubules to decrease blood flow and increase primary urine reabsorption, respectively. Synaptic vesicles release neurotransmitters that activate their effector tissues. However, the mechanisms by which neurotransmitters exert individual responses to renal effector cells remain unknown. Here, we investigated the spatial and molecular compositional associations of renal Schwann cells (SC) supporting the nerve terminals in male rats. The nerve terminals of vascular smooth muscle cells (SMCs) enclosed by renal SC processes were exposed through windows facing the effectors with presynaptic specializations. We found that the adrenergic receptors (ARs) α2A, α2C, and β2 were localized in the SMC and the basal side of the tubules, where the nerve terminals were attached, whereas the other subtypes of ARs were distributed in the glomerular and luminal side, where the norepinephrine released from nerve endings may have indirect access to ARs. In addition, integrins α4 and β1 were coexpressed in the nerve terminals. Thus, renal nerve terminals could contact their effectors via integrins and may have a structure, covered by SC processes, suitable for intensive and directional release of neurotransmitters into the blood, rather than specialized structures in the postsynaptic region.
  Citation: Journal of Histochemistry & Cytochemistry
  PubDate: 2022-06-16T01:53:27Z
  DOI: 10.1369/00221554221106812
Skeletal Muscle Satellite Cells in Sickle Cell Disease Patients and Their
Responses to a Moderate-intensity Endurance Exercise Training Program
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  Authors: Léa Januel, Angèle N. Merlet, Zhiguo He, Christophe Hourdé, Pablo Bartolucci, Barnabas Gellen, Frédéric Galactéros, Laurent A. Messonnier, Léonard Féasson
  First page: 415
  Abstract: Journal of Histochemistry & Cytochemistry, Ahead of Print.
  We previously demonstrated that 8 weeks of moderate-intensity endurance training is safe and improves muscle function and characteristics of sickle cell disease (SCD) patients. Here, we investigated skeletal muscle satellite cells (SCs) in SCD patients and their responses to a training program. Fifteen patients followed the training program while 18 control patients maintained a normal lifestyle. Biopsies of the vastus lateralis muscle were performed before and after training. After training, the cross-sectional area and myonuclear content in type I fibers were slightly increased in the training patients compared to non-training patients. The SC pool was unchanged in type I fibers while it was slightly decreased in type II fibers in the training patients compared to non-training patients. No necrotic fibers were detected in patients before or after training. Therefore, the slight myonuclear accretion in type I fibers in trained SCD patients may highlight the contribution of SCs to training-induced slight type I fiber hypertrophy without expansion of the SC pool. The low training intensity and the short duration of training sessions could explain the low SC response to the training program. However, the lack of necrotic fibers suggests that the training program seemed to be safe for patients’ muscle tissue.
  Citation: Journal of Histochemistry & Cytochemistry
  PubDate: 2022-06-01T05:35:35Z
  DOI: 10.1369/00221554221103905
Heavy Metal Enhancement Technique for Diaminobenzidine in
Immunohistochemistry Enables Ultrastructural Observation by Low-vacuum
Scanning Electron Microscopy
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  Authors: Yutaka Arai, Kazuhiro Takeuchi, Saeko Hatanaka, Arimi Ishikawa, Taichi Inoue, Shoichiro Takakuma, Yusuke Kajimoto, Etsuko Toda, Shinobu kunugi, Mika Terasaki, Akira Shimizu
  First page: 427
  Abstract: Journal of Histochemistry & Cytochemistry, Ahead of Print.
  Low-vacuum scanning electron microscopy (LV-SEM) is a powerful tool that allows to observe light microscopic specimens with periodic acid-silver methenamine (PAM) staining at a higher magnification, simply by removing the coverslip. However, it is not suitable for observation of immunohistochemistry (IHC) using 3,3′-diaminobenzidine (DAB) due to insufficient backscattered electron image. Traditional heavy metal enhancement techniques for DAB in IHC, (1) osmium tetroxide and iron, (2) cobalt, (3) methenamine silver (Ag), (4) gold chloride (Gold), and (5) both Ag and Gold (Ag + Gold), were examined by LV-SEM. Tissue specimens from Thy1.1 glomerulonephritis rat kidney stained with α-smooth muscle actin and visualized with DAB were enhanced by each of these enhancement methods. We found, in light microscopic and LV-SEM, that the enhancement with Ag, Gold, or Ag + Gold had better intensity and contrast than others. At a higher magnification, Ag + Gold enhancement showed high intensity and low background, although only Ag or Gold enhancement had nonspecific background. Even after observation by LV-SEM, the quality of specimens was maintained after remounting the coverslip. It was also confirmed that Ag + Gold enhancement could be useful for IHC using clinical human renal biopsy. These findings indicate that Ag + Gold provided an adequate enhancement in IHC for both LM and LV SEM observation.
  Citation: Journal of Histochemistry & Cytochemistry
  PubDate: 2022-05-25T08:09:24Z
  DOI: 10.1369/00221554221102996
GLUT1, LDHA, and MCT4 Expression Is Deregulated in Cervical Cancer and
Precursor Lesions
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  Authors: Ma. A. Reyna-Hernández, Luz del C. Alarcón-Romero, Julio Ortiz-Ortiz, Berenice Illades-Aguiar, Marco A. Jiménez-López, Azucena Ocampo-Bárcenas, Martin O. Morrugares-Ixtepan, Francisco I. Torres-Rojas
  First page: 437
  Abstract: Journal of Histochemistry & Cytochemistry, Ahead of Print.
  Metabolic reprogramming is typical in cancerous cells and is required for proliferation and cellular survival. In addition, oncoproteins of high-risk human papillomavirus (HR-HPV) are involved in this process. This study evaluated the relationship between glucose transporter I (GLUT1), lactate dehydrogenase A (LDHA), and monocarboxylate transporter type 4 (MCT4) expression and cervical intraepithelial neoplasia (CIN) and invasive cervical carcinoma (ICC) with HR-HPV infection. The protein expression was evaluated in women with CIN I (n=20), CIN II/III (n=16), or ICC (n=24) by immunohistochemistry. The protein expression was analyzed qualitatively by van Zummeren score and quantitatively by Image ProPlus 6 software. LDHA expression increases in HPV-16 infection. In the CIN I group, GLUT1 immunostaining has a 35% protein expression at the membrane level at more than two thirds of the epithelium, which increased by 21.25% more in CIN II/III in more than two thirds of the epithelium. While LDHA and MCT4 in CIN I mostly do not present immunostaining, or this was only limited to the basal stratum, this expression is increased in CIN II/III and ICC cases. The GLUT1, LDHA, and MCT4 expression increased in ICC. The overexpression in high-grade CIN with HR-HPV infection shows a higher risk for cervical carcinoma progression.
  Citation: Journal of Histochemistry & Cytochemistry
  PubDate: 2022-05-26T07:30:42Z
  DOI: 10.1369/00221554221101662
Intracellular Redistribution of Left Ventricular Connexin 43 Contributes
to the Remodeling of Electrical Properties of the Heart in
Insulin-resistant Elderly Rats
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  Authors: Deniz Billur, Yusuf Olgar, Belma Turan
  First page: 447
  Abstract: Journal of Histochemistry & Cytochemistry, Ahead of Print.
  The correlation between long-QT and connexin 43 (Cx43) status and localization in elderly rats was determined to demonstrate a correlation between insulin resistance (I-R), ischemia-reperfusion, aging, and heart dysfunction. Male Wistar rats are grouped as 24-month-old rats (Aged-group), those with metabolic syndrome (8 months old; MetS-group), or controls (8 months old; Con-group). Both experimental groups have long-QT and low heart rate. Immunohistochemical imaging and quantification showed marked decreases in Cx43 staining of intercalated disc with less localizations in the Aged-group and MetS-group. The lateralization of Cx43 on longitudinal cell membrane was significantly high in the MetS-group than in the Con-group with no significant change in the Aged-group. Its significant cytoplasmic internalization was higher in the Aged-group than in the MetS-group. There were marked decreases in phospho-Cx43 (pCx43) staining of intercalated disc with less localizations in both groups than in the Con-group. Furthermore, lateralization of pCx43 was significantly low in the Aged-group and MetS-group, whereas there were no significant changes in the cytoplasmic internalization of both groups compared with the Con-group. Furthermore, the ratio of pCx43 to Cx43 was significantly small in both groups. We determined increases in RhoA and endothelin-1 in both groups, further supporting decreases in pCx43. Our data indicate the important role of I-R on long-QT in aging heart through alterations in both Cx43 protein level and localizations, leading to an abnormal spreading of ventricular repolarization in I-R heart:
  Citation: Journal of Histochemistry & Cytochemistry
  PubDate: 2022-05-24T01:48:23Z
  DOI: 10.1369/00221554221101661