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CYTOLOGY AND HISTOLOGY (32 journals)

Showing 1 - 29 of 29 Journals sorted alphabetically
Acta Histochemica     Hybrid Journal   (Followers: 2)
Annals of Cytology and Pathology     Open Access   (Followers: 1)
Applied Immunohistochemistry & Molecular Morphology     Hybrid Journal   (Followers: 13)
Cell Discovery     Open Access   (Followers: 2)
Comparative Cytogenetics     Open Access   (Followers: 1)
Current Protocols in Cytometry     Hybrid Journal  
Cytogenetic and Genome Research     Full-text available via subscription   (Followers: 1)
Cytokine     Hybrid Journal   (Followers: 2)
Cytokine & Growth Factor Reviews     Hybrid Journal  
Cytokine : X     Open Access   (Followers: 1)
Cytology and Genetics     Hybrid Journal   (Followers: 4)
Cytometry Part A     Hybrid Journal   (Followers: 3)
Cytometry Part B: Clinical Cytometry     Hybrid Journal   (Followers: 4)
Cytopathology     Hybrid Journal   (Followers: 10)
Cytoskeleton     Hybrid Journal   (Followers: 1)
Cytotechnology     Hybrid Journal   (Followers: 4)
Diagnostic Cytopathology     Hybrid Journal   (Followers: 9)
Egyptian Journal of Genetics And Cytology     Open Access  
European Journal of Histochemistry     Open Access   (Followers: 3)
Folia Cryptogamica Estonica     Open Access  
Histochemistry and Cell Biology     Hybrid Journal   (Followers: 5)
Journal of Cytology & Histology     Open Access   (Followers: 4)
Journal of Histochemistry and Cytochemistry     Hybrid Journal   (Followers: 6)
Journal of Histotechnology     Hybrid Journal   (Followers: 1)
Journal of Molecular Histology     Hybrid Journal   (Followers: 4)
Journal of the American Society of Cytopathology     Hybrid Journal   (Followers: 4)
Journal of the History of Biology     Hybrid Journal   (Followers: 5)
Single Cell Biology     Open Access  
Vegetation History and Archaeobotany     Hybrid Journal   (Followers: 4)
Similar Journals
Journal Cover
Cytology and Genetics
Journal Prestige (SJR): 0.161
Number of Followers: 4  
 
  Hybrid Journal Hybrid journal (It can contain Open Access articles)
ISSN (Print) 1934-9440 - ISSN (Online) 0095-4527
Published by Springer-Verlag Homepage  [2468 journals]
  • Erratum to: Computational Identification of Citrus reticulata L. microRNAs
           and the Cis-Acting Regulatory Elements to Predict the Expression
           Probability of Their Respective MIR Genes

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      Abstract: An Erratum to this paper has been published: https://doi.org/10.3103/S0095452723060117
      PubDate: 2023-12-01
       
  • Erratum to: Remodeling the Architecture of Collagen-Containing Connective
           Tissue Fibers of Metastatic Prostate Cancer

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      Abstract: An Erratum to this paper has been published: https://doi.org/10.3103/S0095452723060129
      PubDate: 2023-12-01
       
  • Use of RNA Interference Technology for Improving Economically Valuable
           Traits of Cereal Crops

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      Abstract: RNA interference (RNAi) is a new potential tool for plant breeding by introducing small noncoding RNA sequences with the possibility of silencing gene expression in a sequence-specific manner. The ability to decrease the expression of a certain gene provides the possibility of acquiring a new characteristic through the elimination or accumulation of certain plant traits, which leads to biochemical or phenotypic changes that the original plants do not have. A progress (reached over the past decades) in the application of RNAi for the creation of cereal crops with improved economically valuable traits is described in this literature review. The main stages of the mechanism of gene silencing mediated by short interfering RNAs (siRNAs), peculiarities of their biogenesis, mode of action, and distribution are briefly presented. Numerous examples of the development of different biotechnological approaches to the improvement of cereals using gene transformation and exogenous double-stranded RNA (dsRNA) molecules are summarized. The possibilities of using RNAi technology for changing the agronomic traits of plants, increasing the nutritional value and quality of the grain, and reducing the number of toxic compounds and allergens are highlighted. Considerable attention is paid to the practical results of different applications of RNAi to increase the resistance of grain crops to biotic stress factors (particularly, viruses, bacteria, fungi, pest insects, and nematodes). The examples of using siRNA-mediated RNAi to improve the cereal resistance to abiotic stresses (including drought and salinity) are given.
      PubDate: 2023-12-01
       
  • Development of Winter Common Wheat Lines with the Stem Rust Resistance
           Gene Sr33

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      Abstract: Abstract Stem rust caused by the fungus Puccinia graminis Pers. is a dangerous disease of wheat that occurs in all regions of its cultivation. New highly virulent races causing severe yield losses have appeared in recent decades. Sr33 introgressed from Aegilops tauschii is one of the genes conferring resistance against most races of stem rust, including Ug99. To develop winter common wheat lines with the gene Sr33 and evaluate a possible effect of the gene on yield traits, the authors made a cross between the spring line DH31 carrying the Sr33 gene and the winter cultivar Myrkhad followed by marker-assisted selection of winter genotypes with Sr33 starting from F3. To identify the Sr33 gene, PCR with the gene-specific marker Sr33A was used. Alleles at the storage protein loci of the parental forms were identified using acid polyacrylamide gel electrophoresis and SDS-electrophoresis as well as the molecular marker MAR for Glu-Blal. As a result of marker-assisted selection, winter F5 lines with the Sr33 gene were developed from the cross DH31 × Myrkhad. The yield traits of F5 spikes of families derived from single F3 spikes with and without Sr33 were analyzed considering that the line DH31 has a specific allele at Gli-D1 from Ae. tauschii, dark glumes, and high molecular weight glutenin subunit alleles associated with high dough strength, in particular, Glu-Blal. Comparison of means of yield traits of spikes from families with Sr33 and without it did not reveal significant differences between these two groups. Thus, winter F5 lines with the Sr33 gene from the cross DH31 × Myrkhad may be used in the breeding practice to develop cultivars with high bread-making qualities of flour and stem rust resistance.
      PubDate: 2023-12-01
       
  • Activity of Nonnucleoside Inhibitors of O6-methylguanine-DNA
           Methyltransferase Repair Enzyme in Human Cells In Vitro

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      Abstract: The repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates alkyl lesions that play the main anticancer role in alkylating chemotherapy. The inhibition of MGMT leads to increasing effectiveness of alkylating chemotherapy. In this study, new potential MGMT inhibitors were tested. It was found that some compounds demonstrate low cytotoxicity and high effectiveness in human cells in vitro.
      PubDate: 2023-12-01
       
  • Genotyping of Interspecific Brassica rapa Hybrids Implying β-Tubulin Gene
           Intron Length Polymorphism (TBP/cTBP) Assessment

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      Abstract: Abstract The Crucifers family (Brassicaceae) includes a large number of economically important crops, particularly Brassica rapa, which is a widely used model plant for molecular genetic studies of oilseeds. B. rapa is a highly polymorphic species that includes many of genetically distinct subspecies. Considering this fact, the intraspecific hybridization of B. rapa subspecies is considered a promising breeding approach aimed at increasing the genetic diversity of the crop. Previously, the authors have shown that one of such hybrids, B. rapa subsp. oleifera f. biennis × (subsp. rapifera × pekinensis), could be a valuable oil feedstock due to its increased productivity. However, obtaining hybrids and their subsequent breeding would require the involvement of various molecular marker systems. So far, the method of estimating the length polymorphism of the first (TBP) and second (cTBP) introns of β-tubulin has demonstrated its high accuracy and reliability in the identification (DNA-barcoding) of flowering plant taxonomic units at different levels. In the present study, the productivity of such hybrid oil tyfon (B. rapa subsp. oleifera f. biennis × (subsp. rapifera × pekinensis)) was evaluated and DNA-barcoding of different hybrid tyfon lines (B. rapa subsp. oleifera f. biennis × (subsp. rapifera × pekinensis)) and its parental B. rapa subspecies using the β-tubulin intron length polymorphism assessment approach was carried out. Based on the data of the molecular genetic analysis, which included the assessment of length polymorphism of the first and second introns of β-tubulin genes, we were able to confirm the origin of the oil tyfon hybrid (B. rapa subsp. oleifera f. biennis × (subsp. rapifera × pekinensis)) hybrid from Dutch leaf tyfon (B. rapa subsp. rapifera × pekinensis) and winter turnip (B. rapa subsp. oleifera) with high confidence. Along with that, it was possible to differentiate var. glabra and var. laxa accession of napa cabbage (B. rapa subsp. pekinensis) for the first time using combined TBP and cTBP analyses. A variation in the number of amplified regions of β-tubulin introns was noted in different genotypes; however, these differences did not appear to be a specific feature of a particular subspecies/hybrid. This suggests that B. rapa hybrids most likely do not differ in ploidy compared to their parental genotypes. In addition, it was shown that the mentioned oil tyfon hybrid lines of Ukrainian breeding show a significant level of morphological variation despite their common breeding pedigree.
      PubDate: 2023-12-01
       
  • Distribution of Genotypes for the rs12979860 Polymorphism of the IFNL Gene
           among Children with COVID-19 in Ukraine

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      Abstract: Abstract The coronavirus disease (COVID-19), which is caused by a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), was for the first time detected in December 2019. At the beginning of the pandemic, it was believed that children are less susceptible to COVID-19 compared to adults, but further studies demonstrated that children are also susceptible to infection with the SARS-CoV-2 virus. In recent years, appeared studies about the role of genetic factors in the course of COVID-19. This fact suggests a possible existence of hereditary predisposition of individuals to infection with the SARS-CoV-2 virus. Recently, data was obtained that certain genetic polymorphisms (particularly, different genotypes for the polymorphic variant rs12979860 of the IFNL gene) can act as predictors of the severe course of respiratory infections in children (particularly, COVID-19). The aim of this work was to study the peculiarities of the genotype distribution for the IFNL gene rs12979860 polymorphism in a cohort of children who suffered from COVID-19 as well as to estimate the association of this polymorphism with a risk of infection with the SARS-CoV-2 virus, the development of pneumonia during the coronavirus disease, and the course of this disease among children with recurrent respiratory infections (RRI). For this purpose, genotyping for the IFNL gene rs12979860 locus polymorphism was carried out in the studied group of 70 children who had a laboratory-confirmed COVID-19. According to the results of the study, it was established that the C allele was more common in children with RRI as compared with those with episodic viral infections (p < 0.05, OR 3.2; CI 1.52–6.71); therefore, this variant can be considered as a risk allele for more frequent viral infections. In addition, the C allele predominated in the subgroup of children with pneumonia (p < 0.05, OR 2.36; CI 1.19–4.68), indicating that the C allele can be considered a risk allele for a more severe course of COVID-19 due to pneumonia. The results obtained suggest that the C allele can act as a predictive marker of the risk of developing pneumonia in children with COVID-19. In addition, the carriage of the C allele is associated with cases of RRI among children.
      PubDate: 2023-12-01
       
  • Synergistic Anti-Cancer Potential of Phenethyl Isothiocyanate and Curcumin
           Induces Apoptosis and G2/M Cell Cycle Arrest in HER2-Positive Breast
           Cancer Cells

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      Abstract: HER2 expression is associated with 30% of breast cancer patients with a poor prognosis. Though Trastuzumab is approved for HER2 targeted therapy, its use is limited because of its systemic toxicity and resistance in most patients. This study evaluated the synergistic effects of Phenethyl isothiocyanate (PEITC) and Curcumin (CUR) in HER2 overexpressing SK-BR-3, BT-474, and AU-565 breast cancer cells. The cytotoxic effect of PEITC : CUR against breast cancer cells was evaluated using an MTT assay, and the Loewe additivity model was used to evaluate the synergistic effect. Apoptosis induction and cell cycle arrest over the treatment of PEITC: CUR in breast cancer cells were examined using the flow cytometric annexin-V/Propidium iodide method. Downregulation of HER2-mediated signaling was deduced from protein expression analysis using western-blot. Our results showed that treatment of PEITC : CUR at varying levels of combinations in all three breast cancer cells extensively reduced the survival of the cells with the lowest inhibitory concentrations (IC50). Cytotoxic data revealed that the 3 : 1 ratio of PEITC : CUR was the best among several (1 : 1, 3 : 1, and 1 : 3) combinations, with the maximum cytotoxicity. PEITC : CUR (3 : 1) displayed the lowest combination index (CI) against SK-BR-3, and AU-565 cells indicated its potential synergistic effect. At twice the concentration of its IC50, the 3 : 1 combination elicited 3.5 to 4.5 fold apoptosis in HER2 overexpressing cells, approximately double the effect of the individual drugs alone. In addition, the selected combination induced the G2/M cell cycle arrest in HER2 expressing cells over the treatment. Western blot protein expression analysis revealed that the PEITC : CUR combination suppressed the HER2/PI3K/Akt signaling, eventually connected to various apoptotic biological events. Our results showed the specificity of PEITC: CUR combination in inducing apoptosis and G2/M cell cycle arrest in HER2-expressing tumor cells in-vitro and enhancing the anti-cancer effect. For a subset of breast cancer patients who overexpress HER2, this combination of PEITC and CUR could be a potential treatment option.
      PubDate: 2023-12-01
       
  • 5S Ribosomal DNA in the Family Plumbaginaceae

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      Abstract: Tandemly arranged repetitive regions (repeats) that encode 5S rRNA (5S rDNA) are an indispensable component of eukaryotic genomes. Typically, 5S rDNA repeats within a genome are very similar due to the concerted nature of the evolution of this type of repeats. Each 5S rDNA repeat consists of an evolutionarily conserved coding sequence (CDS) and a variable intergenic spacer (IGS). 5S rDNA is a popular model for studying the molecular evolution of repetitive sequences, and the high rate of IGS mutations determines its wide use in phylogenetic analysis of closely related taxa. Nevertheless, 5S rDNA remains unexplored for many groups of higher plants, especially for the Plumbaginaceae family. Some taxa of this family are endemic to southern Ukraine and listed in the Red Book. However, their taxonomic status is controversial, and its clarification requires the use of molecular phylogenetic methods. In this work, we examined the molecular organization of 5S rDNA for representatives of four genera of the tribe Limonieae, the largest in the family Plumbaginaceae. It was shown that the CDS of 5S rDNA of representatives of the genera Limonium, Armeria, and Ceratolimon possess single mutations that do not affect the formation of the secondary structure of 5S rRNA. In contrast, in the genomes of Goniolimon species, in addition to functionally normal 5S rDNA repeats, numerous pseudogenes were found that do not evolve in a concerted manner and contain numerous mutations in the CDS that disrupt the secondary structure of 5S rRNA. A significant phylogenetic distance between representatives of the subgenera Pteroclados and Limonium of the genus Limonium indicates that Pteroclados can be considered a separate genus. The high rate of molecular evolution makes 5S rDNA IGS a convenient tool for the reconstruction of phylogenetic relationships within the studied genera of the tribe Limonieae and the barcoding of Ukrainian endemics of the genus Limonium.
      PubDate: 2023-12-01
       
  • Transition Bias and Its Compensation in the Evolutionary Lineage of the
           Subfamily Murinae (Rodentia): Analysis of Nuclear and Mitochondrial DNA
           Markers

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      Abstract: Abstract A comparative analysis of the rates of molecular evolution, transition bias, and its evolutionary compensation was carried out on mitochondrial (D-loop, Cytb, COI, 12S RNA) and nuclear (IRBP, Fv) DNA markers in the Murinae subfamily. According to the levels of variability, the markers can be divided into three classes: (1) hypervariable (D-loop), (2) rapidly evolving (Cytb, COI), and (3) conservative (12S RNA, IRBP, Fv). The nature of nucleotide substitutions appears by the levels of variability. With the D-loop, there is a maximum initial bias, which is already partially compensated for during the early stages of speciation, and completely compensated at the stages of species divergence. The pronounced bias within the Cytb and COI genes is only partially compensated, moreover at the genus levels. The 12S RNA, IRBP, and Fv genes with a low level of transition bias do not show evolutionary compensation as such, and the decrease of the ts/tv index in the evolutionary lineage has a technical character and is a consequence of a relative decrease of the difference in the frequencies of transitions and transversions against the background of an absolute increase in the frequencies of substitutions. The positive relationship between the intensity of nucleotide substitutions, the level of transition bias, and the rates of its evolutionary compensation proves that these phenomena have the same primary basis.
      PubDate: 2023-12-01
       
  • Phylogenetic Relationships among Naked Amoebae Found in Natural Biotopes

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      Abstract: Abstract Using morphological traits and molecular-genetic research methods, the authors have identified 24 species of naked amoeba from natural biotopes. The 18S rRNA gene sequences were obtained for the following species of naked amoeba: Amoeba proteus isolate AP07 (ON907618), Saccamoeba limax isolate SLU_22 (OP894078), Saccamoeba limax isolate SL_Uk19 (OQ520144), Saccamoeba sp. strain IDL777 (MZ079370), Thecamoeba striata isolate THS19 (OQ134482), Thecamoeba striata isolate THS20 (OQ134483), Thecamoeba similis isolate Prut river (OL604177), Thecamoeba similis isolate Baggersee Innsbruck (Baggersee Rossau) (OL604178), Thecamoeba quadrilineata isolate THQD2 (ON398269), Thecamoeba quadrilineata isolate THQA1 (ON398268), Thecamoeba sp. strain THS203 (MZ079371), Stenamoeba stenopodia isolate UKSS7 (OP375108), Stenamoeba stenopodia isolate POLSS7 (OP419588), Korotnevella stella isolate KSD2 (ON398267), Korotnevella stella isolate KSA1 (ON398266), Vexillifera bacillipedes isolate river Dnepr (OK649262), Vannella lata isolate Kamenka river (OL305063), Vannella lata isolate Varta river (OL305064), Vannella sp. strain VLS303 (MZ079372), Vannella simplex isolate Black Sea (OM403052), Vannella simplex isolate Mediterranean Sea (OM403053), Ripella sp. strain RPL100 (MZ079369), Mayorella vespertilioides isolate MV_7 (OP739500), Mayorella sp. isolate MY_7 (OP729930), Acanthamoeba sp. strain ATM123 (MZ079366), Acanthamoeba sp. isolate river Elbe (OK649261), Acanthamoeba polyphaga isolate AcPoly01 (ON908497), Acanthamoeba polyphaga isolate AcPoly15 (ON908496), Acanthamoeba griffini isolate Black sea (OM522832), Acanthamoeba griffini isolate Mediterranean Sea (OM522833), Cochliopodium actinophorum strain COP101 (MZ079367), Cochliopodium minus isolate river Stokhid (OK649264), Cochliopodium sp. strain COP102 (MZ079368), Vahlkampfia avara isolate VA7 (OP179657), Willaertia magna isolate river Teterev (OK649263). All of the naked amoebae species on the phylogenetic tree constructed based on the 18S rRNA gene are located within Amoebozoa and grouped with Tubulinea and Discosea. There are separate groups of freshwater, marine, and terrestrial biotopes; these groups are sister species relative to one another with low results of bootstrap analysis, which shows a low accuracy in the distances of particular amoeba species isolated from different natural biotopes.
      PubDate: 2023-12-01
       
  • Mendelism: Connecting the Dots Across Centuries

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      Abstract: Abstract The year 2022 paid a bicentennial tribute to the phenomenal work of the father of Genetics, Gregor Johann Mendel for deciphering the genetic logic behind the phenotypes. His principles were distilled as the law of segregation and law of independent assortment. His work was rediscovered 34 yr later by H. De Vries, C. Correns, and E. Tschermak and popularized by W. Bateson. While C. Darwin accounted for similarities among organisms through the differences in the form of evolution, G. Mendel accounted for similarities through heredity; the ideological gaps were bridged mathematically by R. Fisher. Later with the test of time, the interaction among researchers paved Mendelian principles into different branches of genetics viz., cytogenetics, molecular genetics, population genetics, quantitative genetics, etc. At present we have landed in the era of genomics and the emerging field of phenomics which have potential to bridge the huge gap between demand and supply in different agro-industrial and allied goods. The need to connect the budding researchers in the field of genetics with Mendelism and its significance, catalyzed our concentrated effort to link Mendelism across the centuries, highlighting its importance and extrapolating the concept of heredity and variation from garden peas to different life forms. In conclusion, as our knowledge on genetics deepens, more insights on underlying mechanisms and subsequent applications will be witnessed.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050067
       
  • Molecular Phylogeny and Genetic Diversity of Carpathian Members of the
           Genus Muscari Inferred from Plastid DNA Sequences

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      Abstract: The genus Muscari Mill. (Asparagaceae Juss.) includes about 80 species distributed in Eurasia, mainly in the Mediterranean region. Recent molecular phylogenetic studies have shown that the taxa belonging to this group form a monophyletic clade and are closely related. However, the phylogeny and status of some taxa of Muscari sensu lato remain controversial. So far, most phylogenetic studies of the genus Muscari have used almost exclusively Mediterranean plant material, while representatives of the genus from the Carpathian region remain unexplored. In this work, we used the sequencing of three regions of chloroplast DNA, psbA-trnH, trnT-L, and trnL-F, to clarify the phylogenetic relationships in the genus Muscari, to assess the genetic polymorphism and taxonomic status of Ukrainian populations of M. botryoides, as well as specimens of other Muscari species from the Carpathian region. According to the results of the phylogenetic analysis, the genus Muscari is a monophyletic group that includes three subgenera: Muscari, Muscarimia, and Pseudomuscari. Leopoldia species have been placed in the subgenus Muscari. Specimens of M. botryoides from Ukraine and Austria together with M. transsilvanicum from Romania and M. serpentinicum/M. sandrasicum from Turkey form the clade “Botryoides”, one of the three main clades identified in the subgenus Muscari. A significant genetic distance between Ukrainian specimens of M. botryoides, specimens of this species from other habitats, and other species of the genus Muscari allows us to consider the Ukrainian specimens of M. botryoides as a new, previously undescribed species. A comparison of the sequences of the investigated regions of the chloroplast genome revealed genetic differences between two groups of Ukrainian populations of M. botryoides, which can be interpreted as the existence of two intraspecific forms.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050079
       
  • Remodeling the Architecture of Collagen-Containing Connective Tissue
           Fibers of Metastatic Prostate Cancer

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      Abstract: Abstract The morphological study was aimed at determining the role of fibrillar organization of the collagen-containing connective tissue of prostate cancer at the stage of neoplastic proliferation, including metastatic spreading into bone tissue. The histological material of 55 patients with prostate cancer, Gleason six to nine malignancy score, without neoplastic proliferation and with metastases into bones, was used. The architectural specificities of collagen-containing connective tissue of neoplasms were determined using Van Gieson’s method. It was found that the remodeling of collagen-containing connective tissue around the epithelial tumor structures with aggressive prostate cancer (Gleason score of eight to nine) resulted in the increase in the percentage of extended and flattened fibrils as compared to curved fibrils; the adjacent and surrounding stroma was notable for the enlarged total area of collagen-containing fibrils, manifestations of desmoplasia, compactization of the location, widening, flattening, and extending. The data obtained demonstrate that the remodeling of collagen-containing connective tissue components of prostate cancer conditions unrestricted migration and invasion of tumor cells, including those expressing the proteins involved in bone tissue remodeling.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050031
       
  • The Long Non-Coding RNA ENST00000494165 Influence Papillary Thyroid Cancer
           Cell Proliferation and Invasion

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      Abstract: Abstract Long noncoding RNAs (lncRNAs) have recently been identified as crucial biomarkers of papillary thyroid cancer (PTC). In this study, we aimed to investigate the biological function and potential clinical role of lncRNA ENST00000494165 in PTC. From January 2019 to December 2019, 226 PTC patients who underwent preoperative thyroid US-fine needle aspiration biopsy(US-FNAB) and confirmed by postoperative pathology were enrolled at our hospital. Thyroid tissues were collected from FNAB samples and stored in the refrigerator at –80°C. qRT-PCR (quantitative reverse transcriptase-polymerase chain reaction) analysis was performed to detect the relative expression level of ENST00000494165. CCK-8 (Cell Counting Kit-8) and colony formation assay were performed to detect the cell proliferation ability. Cell migration and invasion abilities were evaluated by transwell and scratch assay. We also evaluated the relation between the expression level of ENST00000494165 and the clinicopathological features of PTC. Functional assays demonstrated that the cell proliferation, migration and invasion abilities were all promoted in PTC cell lines when ENST00000494165 expression was overexpressed. The expression of ENST00000494165 in the lymph node metastasis group was significantly higher than that in non-lymph node metastasis group. High level expression of ENST00000494165 was significantly associated with lymph node metastasis of PTC (P < 0.001). The overexpression of ENST00000494165 promoted the progression, migration and invasion abilities of PTC and was significantly correlated with lymph node metastasis in PTC. ENST00000494165 could act as a possible promoter gene and a potential biomarker for PTC.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050080
       
  • Protoplast Fusion for Cellular Engineering of the Brassicaceae

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      Abstract: The members of the Brassicaceae family are important oilseed, vegetable and forage crops. The Brassicaceae species are also used for phytoremediation of heavy metal polluted soils, as siderates or ornamental plants. The widening of biodiversity of already existent Brassicaceae cultivars by genetic engineering manipulations can increase tolerance to biotic and abiotic stresses, increase plant productivity and production quality. Protoplast fusion method makes possible artificial synthesis of various somatic hybrids between different plant species. The review presents advances in protoplast fusion of Brassica crops and their wild relatives; main directions of somatic hybridisation in Brassicaceae; the conditions for obtaining and selection of the somatic hybrids; the peculiarities of genetic material inheritance, introgression of economically important traits by somatic hybridization to cultivated Brassicaceae, resynthesis of allotetraploid species; production of cytoplasmic male-sterile (CMS) alloplasmic lines, creation of somatic hybrids for phytoremediation of soils contaminated with heavy metals, and production of transplastomic plants by transfer of genetically modified plastids through protoplast fusion.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050043
       
  • Computational Identification of Citrus reticulata L. microRNAs and the
           Cis-Acting Regulatory Elements to Predict the Expression Probability of
           Their Respective MIR Genes

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      Abstract: Abstract MicroRNA(miRNA), a small non-coding class of RNA that regulates the gene expression, is conserved among several plant species. In the present study, an in-silico approach was adopted to identify miRNA from the known expressed sequences tags (ESTs) of Citrus reticulata L. A total of 17 miRNAs from 23 different ESTs along with their secondary structures and targets were predicted. The identified 63 targets include several transcription factors, proteins that regulate plant growth, development, flowering, and seed development together with stress response. The cis-regulatory element present at the promoter region of the MIR genes of C. reticulata describes their role during light responsiveness, auxin, gibberellins, abscisic acid (ABA), anthocyanin responsiveness, salicylic acid responsiveness, anaerobic induction, circadian control, nitrate dependent regulation of the cell cycle and DNA replication, defense, and stress responsiveness. The present study identifies the miRNAs along with their regulatory elements in C. reticulate. The study will also be useful for the research on identification from the genomic data of different plants and prediction of expression probability of identified MIR genes based on the presence of upstream promoter and other regulatory elements.
      PubDate: 2023-10-01
      DOI: 10.3103/S009545272305002X
       
  • The Distribution Characteristics of Microsatellites in the Transcriptome
           of Periplaneta americana

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      Abstract: Abstract To obtain information of Periplaneta americana, we analyzed the distribution characteristics of microsatellite sequences in the P. americana transcriptome (229 MB) by using MSDBv2.4. The total number of perfect microsatellite sequences was 38 082 and covered about 0.3% of P. americana transcriptome. The cumulative length of microsatellites was 618 138 bp, and the density of microsatellites was 2978.54 bp/Mb. In the different repeat types of the microsatellites, the number of the mononucleotide repeats was 20 002 (accounting for 52.52%), which obviously was the most abundant type. While the trinucleotide, tetranucleotide, dinucleotide, pentanucleotide and hexanucleotide repeats accounted for 24.51, 12.97, 8.13, 1.61 and 0.26%, respectively. The kind of different repeat copy categories in each repeat type was also quite different, such as the A in mononucleotide repeat type, the AG in dinucleotide, the AAT in trinucleotide, AAAT in tetranucleotide, the AAGAA in pentanucleotide, and the CAGTAG in hexanucleotide were the most of each category. The A, T, AC, AG, AT, GT, AAG, AAT, ATC, ATG, ATT, CTT, AAAG and AAAT were the dominant repeat copy categories, the total number of all these types was 29 933, accounting for 78.6% in the total number of microsatellite sequences. These results based on a foundation for developing high polymorphic microsatellites to research the functional genomics, population genetic structure and genetic diversity of P. americana.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050092
       
  • Mechanisms of Intron-Mediated Enhancement of Expression: Welcome to the
           Hotel California

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      Abstract: The phenomenon of the positive influence of introns on the expression of a corresponding gene, which is called intron-mediated enhancement (IME), is characteristic of a wide variety of organisms, including nematodes, insects, mammals, fungi, and plants, and occurs due to an as-yet-undefined fundamental mechanism. IME introns have been used for a long time, in particular, in plant biotechnology. Understanding the mechanisms of this phenomenon allows predicting and easily generating stimulatory introns with the given properties and creating highly advantageous phenotypes. It will also give the greenlight to the use of IME in gene therapy and to improve the production of pharmaceutical proteins. In this review, we analysed previously proposed models of IME functioning mechanisms and identified factors that can directly or indirectly determine IME under different conditions and at different levels of gene expression, such as experimental methods of IME research, regulatory RNAs, sequence properties, intron position and orientation, factors at the levels of DNA, transcription, splicing, mRNA, translation, genes in which IME is detected, tissue specificity, repression and how some factors relate to each other by importance. Since there is no single mechanism of IME, and the effect may differ in different species, when modelling this process, only the cases of IME affecting the same level of expression should be compared with each other, taking into account the experimental conditions. Identifying the biological factors that may determine IME and the relationship between them will help in the future to create a corresponding data set suitable for machine learning and try to solve the mystery of the IME phenomenon using machine learning.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050055
       
  • Molecular Organization and Intragenomic Variability of Intergenic Spacer
           of 5S rRNA Genes in Colobanthus quitensis

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      Abstract: Abstract The intergenic spacer (IGS) of 5S ribosomal RNA genes (5S rDNA) (that are present in the genome of all living organisms) is characterized by a high variability, due to which it is a convenient and widely used object for clarifying the issues of genome evolution, population genetics, systematics, etc. The aim of this work was to study the IGS of 5S rDNA of the Antarctic pearlwort Colobanthus quitensis. The nucleotide sequence was determined by the methods of molecular genetic analysis, and molecular organization of the C. quitensis 5S rDNA IGS was studied. It was demonstrated that it contains the regulatory elements typical for other vascular plants. The existence of at least two classes of 5S rDNA repeats in the individual genome (that differ significantly in the length and nucleotide sequence of IGS) was established. In addition, based on differences in the length and nucleotide sequence, two subclasses of repeats with a long IGS and three subclasses with a short IGS were identified. A comparison of 5S rDNA IGS sequences in C. quitensis and Silene latifolia Poir. (a member of another section of the Caryophyllaceae family) demonstrated significant differences in the structure of a spacer region of 5S rRNA genes (except for its parts containing regulatory elements). In general, data obtained indicate a significant level of intragenomic polymorphism of C. quitensis 5S rDNA IGS.
      PubDate: 2023-10-01
      DOI: 10.3103/S0095452723050018
       
 
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Showing 1 - 29 of 29 Journals sorted alphabetically
Acta Histochemica     Hybrid Journal   (Followers: 2)
Annals of Cytology and Pathology     Open Access   (Followers: 1)
Applied Immunohistochemistry & Molecular Morphology     Hybrid Journal   (Followers: 13)
Cell Discovery     Open Access   (Followers: 2)
Comparative Cytogenetics     Open Access   (Followers: 1)
Current Protocols in Cytometry     Hybrid Journal  
Cytogenetic and Genome Research     Full-text available via subscription   (Followers: 1)
Cytokine     Hybrid Journal   (Followers: 2)
Cytokine & Growth Factor Reviews     Hybrid Journal  
Cytokine : X     Open Access   (Followers: 1)
Cytology and Genetics     Hybrid Journal   (Followers: 4)
Cytometry Part A     Hybrid Journal   (Followers: 3)
Cytometry Part B: Clinical Cytometry     Hybrid Journal   (Followers: 4)
Cytopathology     Hybrid Journal   (Followers: 10)
Cytoskeleton     Hybrid Journal   (Followers: 1)
Cytotechnology     Hybrid Journal   (Followers: 4)
Diagnostic Cytopathology     Hybrid Journal   (Followers: 9)
Egyptian Journal of Genetics And Cytology     Open Access  
European Journal of Histochemistry     Open Access   (Followers: 3)
Folia Cryptogamica Estonica     Open Access  
Histochemistry and Cell Biology     Hybrid Journal   (Followers: 5)
Journal of Cytology & Histology     Open Access   (Followers: 4)
Journal of Histochemistry and Cytochemistry     Hybrid Journal   (Followers: 6)
Journal of Histotechnology     Hybrid Journal   (Followers: 1)
Journal of Molecular Histology     Hybrid Journal   (Followers: 4)
Journal of the American Society of Cytopathology     Hybrid Journal   (Followers: 4)
Journal of the History of Biology     Hybrid Journal   (Followers: 5)
Single Cell Biology     Open Access  
Vegetation History and Archaeobotany     Hybrid Journal   (Followers: 4)
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School of Mathematical and Computer Sciences
Heriot-Watt University
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