Authors:Sri Wahyu Vita, Putri Vidiasari Darsono, Saftia Aryzki First page: 282 Abstract: Background: Acne is an infectious disease that is still a severe problem. The therapy used for acne is the bacterial antibiotic Clindamycin. Still, there is a lot of resistance to these antibiotics, one of which is Staphylococcus epidermidis, so it is necessary to find alternative antibacterial drugs to treat acne. Non-pharmacological therapy often used is jackfruit leaf extract (Artocarpus heterophyllus L.) which contains secondary metabolites such as flavonoids, saponins, and tannins which are thought to be effective as antibacterial agents. Methods: The type of research used is True Experimental with the Posttest-Only Control Group research design. The research method used healthy diffusion and liquid dilution, and then the data were analyzed using ANOVA. Results: It was found that jackfruit leaf extract (Artocarpus heterophyllus L.) had antibacterial activity against Staphylococcus epidermidis with a diameter of inhibition zone at a concentration of 50 ppm of 11.7 mm, a concentration of 75 ppm of 12.1 mm, a concentration of 100 ppm of 12.3 mm and had a minimum inhibitory ability at a concentration of 50 ppm with a p-value of 0.007 in the Kruskall-Wallis Test and the Mann Whitney Test showed a p-value of 0.025. Still, the extract did not have the minimum killing ability. Conclusions: Jackfruit leaf extract can only inhibit the growth of Staphylococcus epidermidis bacteria but cannot kill the growth of these bacteria. PubDate: 2023-01-20 DOI: 10.22236/jbes/6310573 Issue No:Vol. 6, No. 3 (2023)
Authors:Sartika Gunawan Putri, Sutriani Kaliu First page: 288 Abstract: Cocoa plantation waste (Theobroma cacao L.), cocoa leaves are increasingly abundant, with efforts to increase cocoa production by trimming the shape. The Ladongi sub-district in Kolaka Regency, Southeast Sulawesi, which produces the most cocoa beans, is also not spared from this. Cocoa leaves have not been used optimally, even though their phytochemical components have the potential as antibacterial. This study aims to determine the antibacterial activity of cocoa leaf extract (Theobroma cacao L.) against acne-causing Cutibacterium acnes and Staphylococcus epidermidis. The method used is the disc diffusion method (Kirby Bauer). The results showed that cocoa leaf extract had the potential as an antibacterial against acne-causing bacteria, Cutibacterium acnes, and Staphylococcus epidermidis with respectively the best inhibitory concentration of 25%, 19mm, and 22mm. PubDate: 2023-01-20 DOI: 10.22236/jbes/6310267 Issue No:Vol. 6, No. 3 (2023)
Authors:Adnan Adnan, Suhardi Aldi, Akhmad Faqih Dzulkarnain, Sitti Marliyah First page: 304 Abstract: Background: This research is a quasi-experiment whose research design is a posttest-only control group design. This study aims to see the effect of e-LKPD based on science process skills in terms of learning outcomes, learning motivation, and students' metacognitive. All students in class, XI MIPA SMAN 14 Makassar from five classes make up the population. Method: The technique used in taking samples of this research is random sampling. There were 28 students of class MIPA 2 and 28 students of XI MIPA 4 who became the control action as a sample. The test instrument is in the form of multiple choice to assess the ability of learning outcomes and the use of questionnaires in measuring learning motivation and questionnaires to measure students' metacognitive abilities. This study's data analysis technique is a descriptive and inferential statistical analysis using non-parametric tests (Mann Whitney). The descriptive analysis results illustrate that using e-LKPD based on science process skills (KPS) for students has learning outcomes in the high category, a very high category in learning motivation, and metacognitive abilities in the high category. Results: The results of the inferential analysis illustrate significant differences in learning outcomes, learning motivation, and metacognitive abilities of students using KPS-based e-LKD in the experimental class, with the mean rank value in the experimental class being higher than the control class. Conclusion: The results of the KPS-based e-LKPD research significantly influence learning outcomes, learning motivation, and students' metacognitive abilities in biology learning at SMA Negeri 14 Makassar. PubDate: 2023-01-20 DOI: 10.22236/jbes/6310259 Issue No:Vol. 6, No. 3 (2023)
Authors:Erlin Listiyaningsih, Rizkyana Avissa, Shinta Dewi Permata Sari, Wening Tri Mawanti, Dewi Martalena First page: 314 Abstract: Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging from asymptomatic, mild symptoms such as flu-like illness and pneumonia to acute respiratory distress syndrome, which ends in death. Until now, the mechanism of the COVID-19 disease that causes widespread symptoms and the severity and factors that influence it are still unclear. During viral internalization, it needs to be cleaved by the serine protease encoded by the TMPRSS2 gene. It is hypothesized that higher expression of the TMPRSS2 gene causes higher virus internalization into cells, leading to more severe symptoms in patients. Methods: The Single Nucleotide Polymorphism Genotype Test was carried out to prove whether the TMPRSS2 gene affects the severity of COVID-19, as evidenced in other viral respiratory diseases. With a better understanding of gene expression related to this disease, it is hoped that we can better understand the mechanism of COVID-19 and establish better therapies and prevention against it. In this study, 68 COVID-19 patients participated and were categorized into two groups based on their clinical symptoms, namely mild symptoms without symptoms (n=12) and Moderate-Severe symptoms (n=56). PBMC cells were isolated from the patient. Then the DNA was extracted and used as a template in the SNP Genotyping of the TMPRSS2 rs2070788 gene variant. Results: The results showed that 35 samples had A/A homozygous genotypes, 29 A/G heterozygous samples, and 4 G/G homozygous samples. n=29) and heterozygous A/G (n=23), whereas only 4 were homozygous G/G. In addition, the homozygous G/G genotype was only detected in the moderate-severe group. Conclusions: A more significant number of samples from the asymptomatic mild symptom group is needed to statistically prove that homozygous G/G variants or G alleles are generally associated with the severity of COVID-19 patients. PubDate: 2023-01-20 DOI: 10.22236/jbes/6310691 Issue No:Vol. 6, No. 3 (2023)
Authors:Pradnya Paramita, Dewi Sri Rahayuningsih First page: 321 Abstract: Based on performance appraisal data at RSIA Bunda Jakarta in 2016 it shows that the percentage of nurses who have poor performance is 124 people (55.6%) and those who have good performance are 99 people (44.4%). And in 2017 it showed that the percentage of nurses who had poor performance was 64 people (36.2%) and those who had good performance were 113 people (63.8%). The purpose of this study was to determine the relationship between motivation, fatigue, and workload on nurse performance in the Mother and Child Hospital Bunda Jakarta in February 2019. Quantitative analytic research using a cross sectional method. The sample in this study were 72 nurses in the inpatient ward of Mother and Child Hospital Jakarta. The bivariate results showed that there was a significant relationship between motivation (p 0.001), fatigue (p 0.038), and workload (p 0.001). Multivariate results showed that the most dominant variables related to nurse performance were motivation and workload (P<0.005). The workload variable is the most dominant variable with the largest Exp (β) of 5.625. PubDate: 2023-01-20 DOI: 10.22236/jbes/6310689 Issue No:Vol. 6, No. 3 (2023)
Authors:Aulia Nova Kusumaningtyas, Yahdiana Harahap, Abdul Mun'im, Supandi Supandi Pages: 294 - 303 Abstract: Background: In Indonesia, the use of herbal plants in overcoming several health problems shows a fairly high rate. Red ginger is one of the herbs that is widely consumed and empirically has the property of relieving or reducing inflammation. However, as is well known, in general the microbiological contamination of herbs is quite high. To maintain the quality of herbal plants, special treatment is required, to ensure that microbial contamination is within safe limits. This study aims to determine the effect of gamma irradiation on the number of microbial contamination, and the bioactive content of 6,8,10-gingerol; 6-shogaol in 70% ethanol extract of red ginger, and its activity as an anti-inflammatory. Methodes: Samples of 70% ethanol extract of red ginger were irradiated with various doses of 0, 5, 7.5, 10 and 15 KGy. Microbiological contamination is determined in Total Plate Number and Yeast Mold Number. The content of compounds 6,8,10-gingerol and 6-shogaol was observed by high performance liquid method and their anti-inflammatory activity was observed by protein denaturation inhibition (BSA) method. Results: Gamma irradiation at doses of 0, 5, 7.5, 10 and 15 KGy reduced microbial contamination as the exposure dose increased, and did not affect the levels of bioactive 6,8,10-gingerol; 6-shogaol and its anti-inflammatory activity. The anti-inflammatory activity of 70% ethanol extract of red ginger is influenced by the content of bioactive compounds. Conclusion: Gamma irradiation is effective for decontaminating microbiological contaminants, and improving the quality of red ginger, and does not affect the bioactive levels contained and its anti-inflammatory activity (in vivo). PubDate: 2022-12-31 DOI: 10.22236/jbes/6310688 Issue No:Vol. 6, No. 3 (2022)
Authors:Pradnya Paramita, Nuraini First page: 329 Abstract: Background: Coronavirus Disease 2019 (Covid-19) is currently a serious world problem with the number of cases always increasing every day. Methods: To find out the factors related to the incidence of coronavirus disease 2019 (Covid-19) in clinical practice, this research was conducted using a quantitative method equipped with a qualitative one and using a cross-sectional design. A sample of 198 people was selected using random sampling. The research instrument used a questionnaire. Out of 198 visitors at the clinic, Result: the respondents were female 53.0%, age > 18 years 70.2%, high school graduate education 86.4%, high minimum income 78.8%, knowledge good 50%, not densely occupied 86.4%, adequate ventilation 82.2% good room lighting 97.5%, washing hands 52.9%, maintaining cough etiquette 51.9%, wearing a mask 51.4%), not holding large-scale gatherings 80.8%, Not maintaining body immunity 69.2%, good facilities and infrastructure 80.8%, Access to remote services 53.0%, there is family support 59.6%. Conclusion: The chi-square test shows that there is a significant relationship between family support and the incidence of Covid-19, Washing Hands, Wearing a Mask, PSBB. Body Immunity, Cough Ethics, then the qualitative results show that almost all informants said they always prevent COVID-19 by implementing health protocols in the form of wearing masks and keeping a distance. PubDate: 2022-12-31 DOI: 10.22236/jbes/6310690 Issue No:Vol. 6, No. 3 (2022)
Open Access journal
[2 journals]
