Authors:Muh Anas, Warda Murti First page: 99 Abstract: The Minister of Culture and Education instructed universities to deactivate lecture activities in the campus environment and convert them to learning from home. By utilizing virtual and digital technology as a substitute for physical meetings, one of the interesting things to use is the Google Classroom application which is an application that can be used for free by providing features that suit the needs of lecturers and students. The purpose of this study was to determine the effectiveness of the Google Classroom learning application on the learning outcomes of students of the Indonesian Development STKIP Biology Study Program. This research is a quantitative research with experimental method with One Group Pretest Posttest Design. The sample in this study was 38 students of STKIP PI biology education in the academic year 2020/2021 in the Vertebrate Zoology course. Data collection techniques in this study are using essay tests, questionnaires, and documentation. The results of this study indicate that the average student biology learning outcome after being taught using the learning model is 80, higher than the student before being given treatment, which is 65.8. The results of hypothesis testing with independent sample t-test obtained tcount value of 3,149 which is higher than ttable of 1.67 which shows student learning outcomes after using the google classroom application are significantly better than students before the application of the application. Data collection techniques in this study are using essay tests, questionnaires, and documentation. The results of this study indicate that the average student biology learning outcome after being taught using the learning model is 80, higher than the student before being given treatment, which is 65.8. The results of hypothesis testing with independent sample t-test obtained tcount value of 3,149 which is higher than ttable of 1.67 which shows student learning outcomes after using the google classroom application are significantly better than students before the application of the application. Data collection techniques in this study are using essay tests, questionnaires, and documentation. The results of this study indicate that the average student biology learning outcome after being taught using the learning model is 80, higher than the student before being given treatment, which is 65.8. The results of hypothesis testing with independent sample t-test obtained tcount value of 3,149 which is higher than ttable of 1.67 which shows student learning outcomes after using the google classroom application are significantly better than students before the application of the application. higher than students before being treated, namely 65.8. The results of hypothesis testing with independent sample t-test obtained tcount value of 3,149 which is higher than ttable of 1.67 which shows student learning outcomes after using the google classroom application are significantly better than students before the application of the application. higher than students before being treated, namely 65.8. The results of hypothesis testing with independent sample t-test obtained tcount value of 3,149 which is higher than ttable of 1.67 which shows student learning outcomes after using the google classroom application are significantly better than students before the application of the application. PubDate: 2022-01-03 DOI: 10.24042/biosfer.v12i2.9723 Issue No:Vol. 12, No. 2 (2022)
Authors:Muhamad Azmi Dwi Susanto, Okta Fina Arianti First page: 110 Abstract: Sabo Dam Complang is a dam area that is minimally polluted and disturbed so that it has the potential as a natural habitat for various types of dragonflies. Diversity and abundance of dragonflies in a location is one component of biodiversity that plays an important role as a predator to maintain the balance of the food chain in natural ecosystems and as a bioindicator of water quality. This study aims to identify and analyze the diversity and abundance of dragonflies species at Sabo Dam Complang. Samples were taken using the VES (Visual Ecounter Survey) method and 2 transect methods, namely line transects and belt transects. The data that has been obtained were analyzed using the Shannon-Wienner species diversity index (H') and the relative abundance of species (KR). The results of this study show the diversity index value of 2.59 which is included in the relatively medium category. There were 20 species of dragonflies that belonged to 6 families. PubDate: 2021-12-30 DOI: 10.24042/biosfer.v12i2.9883 Issue No:Vol. 12, No. 2 (2021)
Authors:Rizki Amalia Nur, Nurhidayah Nurhidayah First page: 123 Abstract: Tujuan Penelitian ini adalah untuk mengetahui pengaruh pembelajaran model Rotating Trio Exchange (RTE) berbantuan media couple card terhadap hasil belajar biologi SMAS DDI Maros; Metode yang digunakan adalah kuantitatif jenis quasi eksperimen. Bentuk dari metode quasi eksperimen yang digunakan adalah nonequivalent group pretest posttest design; hasil temuan diperoleh hasil uji hipotesis nilai hasil belajar pretest pada kelas kontrol dan kelas eksperimen diperoleh 0,138 dimana Sig > α artinya tidak ada perbedaan yang signifikan atau hasil belajar nilai pretest dari kedua kelas tidaklah berbeda. Sedangkan, pada hasil belajar posttest kelas kontrol dan kelas eksperimen diperoleh nilai pada uji-t posttest diperoleh 0,000 dimana Sig < α artinya kedua kelas mengalami perbedaan yang signifikan sehingga terdapat perbedaan hasil belajar dari kedua kelas. Sehingga dapat disimpulkan bahwa penerapan model pembelajaran Rotating Trio Ecchange (RTE) berbantuan media couple card berpengaruh terhadap hasil belajar siswa di SMAS DDI Maros. PubDate: 2021-12-29 DOI: 10.24042/biosfer.v12i2.9913 Issue No:Vol. 12, No. 2 (2021)
Authors:Khairul Anam, Marlina Kamelia, Indah Faridah First page: 130 Abstract: Tujuan penelitian ini adalah untuk mengetahui potensi vitamin C buah belimbing wuluh ( Averrhoa bilimbi) sebagai bahan penggumpal alami lateks. Penelitian menggunakan metode Rancangan Acak Lengkap (RAL) yaitu terdapat tiga kali buah dengan perlakuan yaitu kontrol negatif (tanpa bahan penggumpal) dan kontrol positif diberikan sari belimbing wuluh ( Averrhoa bilimbi) dengan volume 5 ml, 10 ml, 15 ml dan 20 ml. Uji kadar vitamin C menggunakan Spektofotometri UV-VIS tipe DR 6000 dengan panjang gelombang 265 nm – 271 nm. Selanjutnya data dianalisis menggunakan uji statistik One Way Anovadan dilakukan uji lanjut BNT untuk mengetahui suara yang paling efektif sebagai penggumpal lateks. Data kuantitatif berupa waktu penggumpalan dan berat lateks. Hasil penelitian menunjukkan bahwa, sari buah belimbing wuluh dapat digunakan sebagai lateks koagulan. waktu tercepat dan berat tertinggi lateks terdapat pada volume sari buah 20 ml yaitu 1,18 menit dan 34,30 gram. Sedangkan hasil terendah pada volume 5 ml dengan waktu penggumpalan 5,4 menit dan berat 18,62 gram. PubDate: 2021-12-30 DOI: 10.24042/biosfer.v12i2.7877 Issue No:Vol. 12, No. 2 (2021)
Authors:Swastika Oktavia, Cory Novi First page: 140 Abstract: The purpose of this study was to determine the acute toxicity of household-scale Lebak Batik industrial wastewater on carp. The methods used were waste characteristics test, animal acclimatization, acute toxicity test for 96 hours with 0, 10, 20, 30, 40, and 50% treatments, measurement of environmental parameters, and data analysis using probit analysis. The results showed that the lethal time (LT50) and lethal concentration (LC50) for 96 hours of treatment were 1,8 days and 8,56%, respectively, with a Toxicity Unit Area (TUa) of 11,68. This proved that the wastewater from the Lebak batik industry had a major effect on causing acute toxicity. PubDate: 2021-12-31 DOI: 10.24042/biosfer.v12i2.9737 Issue No:Vol. 12, No. 2 (2021)
Authors:Eva Tyas Utami, Indah Salsabila Kurnia, Mahriani Mahriani First page: 149 Abstract: Aspartame is one of the most widely used artificial sweeteners in food and beverages. In the body, aspartame will undergo metabolism into methanol which will be oxidized to formaldehyde. Formaldehyde will increase the production of ROS. The purpose of this study was to determine the effect of Catharanthus roseus leaves extract on kidney histology of mice aspartame-induced. Mice were divided into 4 groups, namely the negative control group (K-) without treatment, the positive control (K+) given aspartame 0.02 mg/day, the dose group 1 (D1) was given aspartame 0.02 mg/day and continued with the C. roseus leaves extract at a dose of 0.42 mg/kg, and group 2 (D2) were given aspartame 0.02 mg/day followed by C. roseus leaf extract at a dose of 0.84 mg/kg bw. Aspartame was administered by IP for 14 days and C. roseus leaves extract was administered by gavage for 12 days. The data obtained were analyzed by One Way Anova and DMRT test with a significance level of 0.05. The results showed that the induction of aspartame at a dose of 0.02 mg/day in mice increased the average number of proximal tubular necrosis cells. Catharanthus roseus leaves extract administration at 0.42 mg/kg bw and 0.84 mg/kg bw for 12 days can reduce the number of proximal tubular necrosis cells in aspartame-induced mice. Thus it can be concluded that the administration of C. roseus leaves extract can reduce the proximal kidney tubules necrosis but has not been able to reach normal conditions yet. PubDate: 2021-12-31 DOI: 10.24042/biosfer.v12i2.10007 Issue No:Vol. 12, No. 2 (2021)
Authors:Doki Efendi, Meilinda Meilinda, Riyanto Riyanto First page: 158 Abstract: This study aims at finding out the form of indigenous knowledge of the Tebat Benawa Community toward Tebat Bukit conservation. It was conducted from December 2019 to January 2020. It was a descriptive study the object of which was the Tebat Benawa Village Community. The data were collected by means of literature study, observation, and semistructured interviews. The results show that the Community has five indigenous knowledge in the implementation of Tebat Bukit conservation which is classified into three indicators, i.e. a) availability of tebat (artificial lake) water: Tebat Bukit and ghumbus b) Use of tebat water: Siring ayik, c) tebat and its water management: Nebasi jalan, ghumbus, and society knowledge transfer in preserving Tebat Bukit through direct experience sharing, for example taking part in fishing in tebat, participattion in implementing indigenous tradition, socialization of village administration, parental advice, and petatah-petitih (oral tradition on local wisdom) PubDate: 2021-12-31 DOI: 10.24042/biosfer.v12i2.9641 Issue No:Vol. 12, No. 2 (2021)
Authors:Endang Sulistyarini Gultom, Hasruddin Hasruddin, Aida Fitriani Sitompul, Albert D Situmorang, Eko Prasetya First page: 169 Abstract: Irrational use of antibiotics for the treatment of infections in diseases caused by pathogenic bacteria can cause resistance. One way to overcome this resistance is to explore active compounds that have the potential as antibacteria of natural origin, namely sponge symbiont bacteria. This research method is experimental with descriptive data analysis. The purpose of this study was to isolate and identify sponge symbiont bacteria that have antibacterial potential against Multi Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the results of the study, 2 sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the results of the antibacterial activity test, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumonia ESBL, Pseudomonas aeruginosa ESBL, or Stapyhlococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence, isolates S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria that have antibacterial potential against MDRO.
Authors:Nur Khalifah, Riyanto Riyanto, Khiron Nazip, Meilinda Meilinda First page: 185 Abstract: Air merupakan komponen penting yang harus ada di bumi dan tetap harus di jaga kelestariannya dengan konservasi air yang diajarkan sejak dini, melalui sistem belajar yang kompleks pada materi siklus air dan mengaitkannya dengan berpikir sistem dikembangkanlah LKPD berbasis system approach pada topik siklus air di SMP yang di uji kevalidan dan kepraktisannya melalui metode Developmental Research tipe II oleh Richey & Klein (2005) dengan dua tahapan yaitu fase eksplorasi dan uji coba kepada peserta didik. Fase uji coba dilakukan melalui metode lesson study yang diobservasi oleh lima orang observer dengan tujuan untuk mengetahui aktivitas siswa selama pembelajaran berlangsung. Hasil LKPD divalidasi oleh lima orang validator untuk menguji kevalidan LKPD dengan menggunakan skala likert. Teknik analisis data dilakukan dengan analisis data kuantitatif dan kualitatif. Hasil yang diperoleh berupa persentasi uji kevalidan dengan rata-rata78% yang artinya LKPD sudah dalam kategori valid. Kemudian dari hasil uji praktikalitas peserta didik diperoleh hasil rata-rata 81,02% yang menunjukkan kategori sangat praktis. Sehingga di hasilkanlah LKPD yang mempunyai nilai kevalidan dan kepraktisan yang layak digunakan dalam proses pembelajaran siklus air. PubDate: 2021-12-30 DOI: 10.24042/biosfer.v12i2.10467 Issue No:Vol. 12, No. 2 (2021)
Authors:Istiqomah istiqomah, Sri Wahyuningsih, Eti Ernawiati, Rochmah Agustrina First page: 194 Abstract: Colchicine is often used in plant breeding to induce mutations to produce polyploid plants that have superior properties. Polyploid plants are plants that have more than one set of chromosomes in their somatic cells. Cytologically, polyploid plants have a larger variations in shape, the number of chromosomes, and cell size. The use of colchicine compounds in Kepok Abu Banana expected to produce the polyploid of Kepok Abu Banana. The purpose of this study was to determine the emergence of mitotic abnormalities, changes in the number of chromosomes, the mitotic index, and the number and length of plantlet roots of Kepok Abu banana due to 0.1% of colchicine's application on tissue culture media. This research was conducted at the Botanical Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung. This study compared cytology with the Squash method between root tip cells of Kepok Abu banana's plantlets after 0.1% of colchicine addition with control. Data were analyzed descriptively and displayed in tables and bar charts. The results showed that 0.1% of colchicine caused mitotic abnormalities and increased the number of chromosomes. The control group resulted 2n = 3x, and the group with 0.1% of colchicine's treatment is 2n = 3x + 4. The mitotic index decreased, and the average number of roots was less, but the size was longer than the control. It can be concluded that 0.1% of colchicine can induce polyploid cells in Kepok Abu Banana plantlets. PubDate: 2021-12-31 DOI: 10.24042/biosfer.v12i2.8501 Issue No:Vol. 12, No. 2 (2021)
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