Authors:Deb; Debal, Dutta, Sreejata, Erickson, Richard First page: 2 Abstract: Land equivalent ratio (LER) is a most widely used indicator of yield advantage of multi-crop farms over sole-crop farms, and usually measured using crop biomass yield per unit area. Most often, crop yields are compared between both systems using the same area. In this paper, we demonstrate that although the yield per unit area and the yield per plant are widely different, LER remains invariant. As a corollary, area time equivalent ratio and land use efficiency, derived from LER, also remain unchanged when using the two different measures of crop yields. We recommend that when the estimation of the exact land area is difficult due to complex crop planting designs, yield per plant estimate is much easier and equally valid for estimation of LER and its derivative indices. PubDate: 2022-01-13 DOI: 10.1017/exp.2021.33
Authors:Law; Christopher J., Meaney, Steve First page: 3 Abstract: The phenomenon of antimicrobial resistance represents a major public health risk. The activity of integral membrane transporter proteins contributes to antimicrobial resistance in pathogenic bacteria and proton gradient-driven multidrug efflux representatives of the major facilitator superfamily (MFS) of secondary transporters are the dominant antimicrobial efflux proteins in Escherichia coli. In many, but not all, of the characterized MFS multidrug transporters, an aspartic acid residue at position D+5 of the conserved signature Motif A is essential for transport activity. The present work extends those studies to the E. coli MFS multidrug/H+ antiporter MdtM and used a combination of mutagenesis, expression studies, antimicrobial resistance assays, and transport activity measurements to reveal that a negatively charged residue at position D+5 is critical for MdtM transport function. PubDate: 2022-01-13 DOI: 10.1017/exp.2022.1
Authors:Low Ying Si; Eliza, Chadwick, Michael A., Smith, Thomas E. L., Sukmaria Sukri, Rahayu, Adamczyk, Bartosz First page: 4 Abstract: This study quantified CO2 emissions from tropical peat swamp soils in Brunei Darussalam. At each site, soil was collected from areas of intact and degraded peat and CO2 flux, and total organic content were measured ex situ. Soil organic content (~20–99%) was not significantly different between intact and degraded forest samples. CO2 flux was higher for intact forest samples than degraded forest samples (~1.0 vs. ~0.6 μmol CO2 m−2 s−1, respectively) but did not differ among forest locations. From our laboratory experiments, we estimated a potential emissions of ~10–20 t CO2 ha−1 y−1 which is in the lower range of values reported for other tropical peat swamps. However, our results are likely affected by unmeasured variation in root respiration and the lability of resident carbon. Overall, these findings provide experimental evidence to support that clearance of tropical peat swamp forests can increase CO2 emissions due to faster rates of decomposition. PubDate: 2022-01-18 DOI: 10.1017/exp.2022.2
Authors:Azadi; Mohammad, Bahmanabadi, Hamed, Gruen, Florian, Winter, Gerhard, Seisenbacher, Benjamin, Celikin, Mert First page: 6 Abstract: This article presents cyclic hardening/softening behaviors (experimental data) of the heat-treated aluminum-matrix nano-clay-composite (AlSi_N_HT6), compared to those of the piston aluminum alloy (AlSi) under strain-controlled loading. For such an objective, standard samples were fabricated by gravity and stir-casting methods. Low-cycle fatigue experiments were carried out under different strain amplitudes (0.20–0.45%) and at various temperatures (25–300°C). Obtained results implied that no obvious change was observed on material properties of aluminum alloy by reinforcements, but a decrement was observed due to increasing the temperature. Results also indicated that the increase of the temperature from 25°C to 200°C has changed the cyclic behavior of both materials (AlSi_N_HT6 and AlSi) from hardening to softening. Moreover, the temperature effect was more significant than the total strain amplitude influences in cyclic behaviors. PubDate: 2022-02-09 DOI: 10.1017/exp.2021.32
Authors:Matsuoka; Daisuke, Carley, Jacob First page: 7 Abstract: Atmospheric simulation data present richer information in terms of spatiotemporal resolution, spatial dimension, and the number of physical quantities compared to observational data; however, such simulations do not perfectly correspond to the real atmospheric conditions. Additionally, extensive simulation data aids machine learning-based image classification in atmospheric science. In this study, we applied a machine learning model for tropical cyclone detection, which was trained using both simulation and satellite observation data. Consequently, the classification performance was significantly lower than that obtained with the application of simulation data. Owing to the large gap between the simulation and observation data, the classification model could not be practically trained only on the simulation data. Thus, the representation capability of the simulation data must be analyzed and integrated into the observation data for application in real problems. PubDate: 2022-02-24 DOI: 10.1017/exp.2022.3
Authors:Singh; Surendra V., Vishakantaiah, Jayaram, Meka, Jaya K., Muruganantham, Mariyappan, Thiruvenkatam, Vijay, Sivaprahasam, Vijayan, Rajasekhar, Balabhadrapatruni N., Bhardwaj, Anil, Mason, Nigel J., Sivaraman, Bhalamurugan, Beseres Pollack, Jennifer First page: 8 Abstract: Asteroid and cometary impacts have been considered one of the possible routes for exogenous delivery of organics to the early Earth. It is well established that amino acids can be synthesized due to impact-driven shock processesing of simple molecules and that amino acids can survive the extreme conditions of impact events. In the present study, we simulate impact-induced shock conditions utilizing a shock tube that can maintain a reflected shock temperature of about 5,500 K for 2 ms time scale. We have performed shock processing of various combinations of amino acids with subsequent morphological analysis carried out using Scanning Electron Microscope (SEM), revealing that the shock processed amino acids demonstrate an extensive range of complex structures. These results provide evidence for the further evolution of amino acids in impact-induced shock environments leading to the formation of complex structures and thus providing a pathway for the origin of life. PubDate: 2022-02-09 DOI: 10.1017/exp.2021.17
Authors:Sjöbom; Ulrika, Cismaan, Osman, Hansen-Pupp, Ingrid, Wackernagel, Dirk, Sävman, Karin, Hellström, Ann, Nilsson, Anders K., Alarcón López, Francisco Javier First page: 9 Abstract: Preterm infants show postnatal deficits of long-chain polyunsaturated fatty acids (LCPUFAs) which are essential for adequate growth and neurodevelopment. Human milk is a primary source of fatty acids (FAs) for the preterm infant, and therefore, knowledge about milk FA levels is required to design appropriate supplementation strategies. Here, we expanded on our previous study (Nilsson et al., 2018, Acta Paediatrica, 107, 1020–1027) determining FA composition in milk obtained from mothers of extremely low gestational age ( PubDate: 2022-02-24 DOI: 10.1017/exp.2022.4
Authors:Zolnai; Tamás, Dávid, Dominika Réka, Pesthy, Orsolya, Nemeth, Marton, Kiss, Mariann, Nagy, Márton, Nemeth, Dezso, Ergul, Ayca First page: 10 Abstract: Statistical learning—the skill to pick up probability-based regularities of the environment—plays a crucial role in adapting to the environment and learning perceptual, motor, and language skills in healthy and clinical populations. Here, we developed a new method to measure statistical learning without any manual responses. We used the Alternating Serial Reaction Time (ASRT) task, adapted to eye-tracker, which, besides measuring reaction times (RTs), enabled us to track learning-dependent anticipatory eye movements. We found robust, interference-resistant learning on RT; moreover, learning-dependent anticipatory eye movements were even more sensitive measures of statistical learning on this task. Our method provides a way to apply the widely used ASRT task to operationalize statistical learning in clinical populations where the use of manual tasks is hindered, such as in Parkinson’s disease. Furthermore, it also enables future basic research to use a more sensitive version of this task to measure predictive processing. PubDate: 2022-08-15 DOI: 10.1017/exp.2022.8
Authors:Fujita; Masako, Brindle, Eleanor, Nevels, Michael First page: 11 Abstract: ObjectivesThe creamatocrit is a simple technique for estimating the lipid content of milk, widely adopted for clinical and research purposes. We evaluated the effect of long-term cryogenic storage on the creamatocrit for human milk.MethodsFrozen and thawed milk specimens (n = 18) were subjected to the creamatocrit technique. The specimens were reanalyzed after long-term cryogenic storage (10 years at PubDate: 2022-08-24 DOI: 10.1017/exp.2022.9
Authors:Waldren; Lucy H., Livingston, Lucy A., Leung, Florence Y. N., Shah, Punit, Postal, Gregory First page: 12 Abstract: The 10-item Autism-Spectrum Quotient (AQ10) is a measure of autistic traits used in research and clinical practice. Recently, the AQ10 has garnered critical attention, with research questioning its psychometric properties and clinical cutoff value. To help inform the utility of the measure, we conducted the first network analysis of the AQ10, with a view to gain a better understanding of its individual items. Using a large dataset of 6,595 participants who had completed the AQ10, we found strongest inter-subscale connections between communication, imagination, and socially relevant items. The nodes with greatest centrality concerned theory of mind differences. Together, these findings align with cognitive explanations of autism and provide clues about which AQ10 items show greatest utility for informing autism-related clinical practice. PubDate: 2022-07-28 DOI: 10.1017/exp.2022.7
Authors:Malaret; Francisco, Yang, Xin-She First page: 13 Abstract: Corrosion is an important problem that engineers and scientists must overcome to avoid the collapse of structures, chemical processing plants, and metallic objects, which can lead to not only economic loss but also environmental and human losses. One of the simplest and most widely used methods to quantify corrosion rates (CRs) is the immersion test. The usual approach that has been used to date, to quantify the CR by this method, is to assume that the initial surface area of the corroding object remains constant over time. It is shown that such approximations underestimate the true CR and that they may lead to significant errors. A formula to calculate the CR considering changes in the area is presented in this work. The formula herein can be used to accurately quantify the CR by the immersion test and improve the quality of experimental data and the analysis and modeling of corrosion phenomena. PubDate: 2022-05-16 DOI: 10.1017/exp.2022.5
Authors:Atumo; Tessema Tesfaye, Mengesha, Getachew Gudero, Nevels, Michael First page: 14 Abstract: Dual-purpose sorghum response to anthracnose disease, growth, and yield was undertaken in Derashe and Arba Minch trial sites during March–June 2018 and 2019. Five sorghum varieties and Rara (local check) were arranged in a randomized complete block design with four replications. Variety Chelenko exhibited the tallest main crop plant height (430 cm) while Dishkara was the tallest (196.65 cm) at ratoon crop harvesting. Rara had a higher tiller number (main = 6.73, ratoon = 9.73) among the varieties. Dishkara and Chelenko varieties produced 50 and 10% more dry biomass yield (DBY) than the overall mean DBY, while Konoda produced 40% less. Although the anthracnose infestation was highest on the varieties Konoda (percentage severity index [PSI] = 20.37%) and NTJ_2 (PSI = 32.19%), they produced significantly (p PubDate: 2022-08-25 DOI: 10.1017/exp.2022.12
Authors:Vine; Christopher, Coakley, Sarah, Myers, Stephen, Blacker, Sam, Runswick, Oliver, Postal, Gregory First page: 15 Abstract: During military operations, soldiers are required to successfully complete numerous physical and cognitive tasks concurrently. Understanding the typical variance in research tools that may be used to provide insight into the interrelationship between physical and cognitive performance is therefore highly important. This study assessed the inter-day variability of two military-specific cognitive assessments: a Military-Specific Auditory N-Back Task (MSANT) and a Shoot-/Don’t-Shoot Task (SDST) in 28 participants. Limits of agreement ±95% confidence intervals, standard error of the mean, and smallest detectable change were calculated to quantify the typical variance in task performance. All parameters within the MSANT and SDST demonstrated no mean difference for trial visit in either the seated or walking condition, with equivalency demonstrated for the majority of comparisons. Collectively, these data provided an indication of the typical variance in MSANT and SDST performance, while demonstrating that both assessments can be used during seated and walking conditions. PubDate: 2022-08-24 DOI: 10.1017/exp.2022.11
Authors:Marín-Franch; Iván, Sanz-Sabater, Martín, Foster, David H., Frontoni, Emanuele First page: 16 Abstract: Numerical estimators of differential entropy and mutual information can be slow to converge as sample size increases. The offset Kozachenko–Leonenko (KLo) method described here implements an offset version of the Kozachenko–Leonenko estimator that can markedly improve convergence. Its use is illustrated in applications to the comparison of trivariate data from successive scene color images and the comparison of univariate data from stereophonic music tracks. Publicly available code for KLo estimation of both differential entropy and mutual information is provided for R, Python, and MATLAB computing environments at https://github.com/imarinfr/klo. PubDate: 2022-09-05 DOI: 10.1017/exp.2022.14
Authors:Rubiano-Rincon; Sebastian, Larkin, Patrick D., Kim, Il-Nam First page: 17 Abstract: Hydrogen sulfide (H2S, “sulfide”) is a naturally occurring component of the marine sediment. Eutrophication of coastal waters, however, can lead to an excess of sulfide production that can prove toxic to seagrasses. We used stable sulfur isotope ratio (δ34S) measurements to assess sulfide intrusion in the seagrass Halodule wrightii, a semi-tropical species found throughout the Gulf of Mexico, Caribbean Sea, and both western and eastern Atlantic coasts. We found a gradient in δ34S values (−5.58 ± 0.54‰+13.58 ± 0.30‰) from roots to leaves, in accordance with prior observations and those from other species. The results may also represent the first values reported for H. wrightii rhizome tissue. The presence of sulfide-derived sulfur in varying proportions (15–55%) among leaf, rhizome, and root tissues suggests H. wrightii is able to assimilate sedimentary H2S into non-toxic forms that constitute a significant portion of the plant’s total sulfur content. PubDate: 2022-09-28 DOI: 10.1017/exp.2022.15
Authors:Feldmann; Maria, Hagmann, Cornelia, Bernet, Vera, Knirsch, Walter, Latal, Beatrice, Ergul, Ayca First page: 18 Abstract: This study assessed neonatal visual maturity in infants with congenital heart disease (CHD) and its predictive value for neurodevelopmental outcomes. Neonates with CHD underwent a standardized visual assessment before and after cardiopulmonary bypass surgery. Visual maturity was rated as normal versus abnormal by means of normative reference data. Twelve-month neurodevelopment was assessed with the Bayley-III. Twenty-five healthy controls served as the reference group. Neonatal visual assessment was performed in five neonates with CHD preoperatively and in 24 postoperatively. Only postoperative assessments were considered for further analysis. Median [IQR] age at assessment was 27.0 [21.5, 42.0] days of life in postoperative neonates with CHD and 24.0 [15.0, 32.0] in controls. Visual performance was within reference values in 87.5% in postoperative CHD versus 90.5% in healthy controls (p = 1.0). Visual maturity was not predictive of neurodevelopment at 12 months. These results demonstrate the limited feasibility and predictive value of neonatal visual assessments in CHD. PubDate: 2022-08-25 DOI: 10.1017/exp.2022.10
Authors:Oladeji-Atanda; Gbadebo, Mpoeleng, Dimane, Frontoni, Emanuele First page: 19 Abstract: We have previously shown that the geographic routing’s greedy packet forwarding distance (PFD), in dissimilarity values of its average measures, characterizes a mobile ad hoc network’s (MANET) topology by node size. In this article, we demonstrate a distribution-based analysis of the PFD measures that were generated by two representative greedy algorithms, namely GREEDY and ELLIPSOID. The result shows the potential of the distribution-based dissimilarity learning of the PFD in topology characterizing. Characterizing dynamic MANET topology supports context-aware performance optimization in position-based or geographic packet routing. PubDate: 2022-09-19 DOI: 10.1017/exp.2022.19
Authors:Newall; Philip W. S., Walasek, Lukasz, Ludvig, Elliot A., Jenkins, Matthew First page: 20 Abstract: “Return-to-player” information is used in several jurisdictions to display the long-run cost of gambling, but previous evidence suggests that these messages are frequently misunderstood by gamblers. Two ways of improving the communication of return-to-player information have been suggested: switching to an equivalent “house-edge” format, or via the use of a “volatility warning,” clarifying that the information applies only in the statistical long run. In this study, Australian participants (N = 603) were presented with either a standard return-to-player message, the same message supplemented with a volatility warning, or a house-edge message. The return-to-player plus volatility warning message was understood correctly more frequently than the return-to-player message, but the house-edge message was understood best of all. Participants perceived the lowest chance of winning in the return-to-player plus volatility warning condition. These findings contribute data on the relative merits of two proposed approaches in the design of improved gambling information. PubDate: 2022-09-27 DOI: 10.1017/exp.2022.21
Authors:Shimizu; Yuho, Grassi, Massimo First page: 21 Abstract: Reducing negative attitudes toward older adults is an urgent issue. A previous study has conducted “stereotype embodiment theory”-based interventions (SET interventions) that present participants with the contents of SET and related empirical findings. I focus on the subjective time to become older (the perception of how long people feel it will be before they become old) as a mechanism for the effect of SET interventions. I make the SET intervention group and the control group in which the participants are presented with an irrelevant vignette. The data from 641 participants (M = 31.97 years) were analyzed. Consequently, the SET intervention shortened the subjective time to become older and reduced negative attitudes toward older adults. When considering SET interventions, it would be useful to focus not only on the self-interested motives to avoid age discrimination but also on the subjective time to become older. PubDate: 2022-09-19 DOI: 10.1017/exp.2022.18
Authors:O’Leary; Tadhg, McCarron, Pauraic, Devereux, Michael, Meaney, Steve, Guler, Eray Metin First page: 22 Abstract: The unfolded protein response has recently been implicated as a mechanism by which 1,10-phenanthroline-containing coordination compounds trigger cell death. We explored the interaction of two such compounds—one containing copper and one containing manganese—with endoplasmic reticulum (ER) stress. Pretreatment with anisomycin significantly enhanced the cytotoxic activity of both metal-based compounds in A2780, but only the copper-based compound in A549 cells. The effects of pretreatment with tunicamycin were dependent on the nature of the metal center in the compounds. In A2780 cells, the cytotoxic action of the copper compound was reduced by tunicamycin only at high concentration. In contrast, in A549 cells the efficacy of the manganese compound cells was reduced at all tested concentrations. Intriguingly, some impact of free 1,10-phenanthroline was also observed in A549 cells. These results are discussed in the context of the emerging evidence that the ER plays a role in the cytotoxic action of 1,10-phenanthroline-based compounds. PubDate: 2022-09-26 DOI: 10.1017/exp.2022.20
Authors:Raadam; Bo, Matlock, David K., Celikin, Mert First page: 23 Abstract: Digital image correlation (DIC) techniques were used to evaluate strain distributions along tensile gage lengths immediately after yielding of a medium manganese steel (7 wt% Mn) in samples cold rolled in the range of 1–6 pct. With an increase in cold work, DIC confirmed that the yielding behavior transitioned from nucleation and propagation of a single localized deformation zone (Lüders band) to uniform deformation, that is, no evidence of strain localization. At intermediate amounts of cold work, a unique yielding behavior was evident where the initially-low positive strain hardening rate increased with tensile strain until conventional strain hardening (i.e., decrease in strain hardening rate with strain). The intermediate yielding behavior was associated with the development of multiple non‑propagating regions of strain localization, an observation not previously evident without the use of DIC. PubDate: 2022-10-19 DOI: 10.1017/exp.2022.22
Authors:Schellhas; Dan, Green, Robert C., Francisco, Vitor First page: 24 Abstract: The distribution of human leukocyte antigens in the population assists in matching solid organ donors and recipients when the typing methods used do not provide sufficiently precise information. This is made possible by linkage disequilibrium (LD), where alleles co-occur more often than random chance would suggest. There is a trade-off between the high bias and low variance of a broad sample from the population and the low bias but high variance of a focused sample. Some of this trade-off could be alleviated if sub-populations shared LD despite having different allele frequencies. These experiments show that Bayesian estimation can balance bias and variance by tuning the effective sample size of the reference panel, but the LD as represented by an additive or multiplicative copula is not shared. PubDate: 2022-10-10 DOI: 10.1017/exp.2022.17
Authors:Jimenez-Torres; Jesus A., Monroy-Gonzalez, Zurisadai, Juarez-Muñoz, Juana, Jørgensen, Bodil First page: 25 Abstract: Sexual propagation of Agave plants is an incipient cultivation method, these plants withstand drought and adverse growing conditions; therefore, research on Agave’s diversity, seed processing, and storage could support its cultivation on marginal lands. The aim of this work was to evaluate seed morphology, germination, and seedling genetic diversity of six seed origins (species × provenance) of Agave plants collected in five provenances from Mexico. Seed longevity was evaluated in two seed origins after a 10-year storage period. Seed morphology and seedling genetic variation results demonstrated intra- and interspecific variation within Agave salmiana and with the other seed origins, respectively. After a 10-year storage period seed germination of two A. salmiana seed origins remained relatively stable, storage conditions, and seed variables of this work can serve as reference parameters for future analyses. To the best authors’ knowledge, this is the first report of Agave’s seed longevity evaluation after a 10-year storage period. PubDate: 2022-09-13 DOI: 10.1017/exp.2022.16
Authors:Hillier; Andrew, Takasao, Shinsuke, Camera, Stefano First page: 26 Abstract: The short timescale of the solar flare reconnection process has long proved to be a puzzle. Recent studies suggest the importance of the formation of plasmoids in the reconnecting current sheet, with quantifying the aspect ratio of the width to length of the current sheet in terms of a negative power of the Lundquist number, that is, , being key to understanding the onset of plasmoids formation. In this paper, we make the first application of theoretical scalings for this aspect ratio to observed flares to evaluate how plasmoid formation may connect with observations. For three different flares that show plasmoids we find a range of values of to . The values in this small range implies that plasmoids may be forming before the theoretically predicted critical aspect ratio () has been reached, potentially presenting a challenge for the theoretical models. PubDate: 2022-11-25 DOI: 10.1017/exp.2022.23
Authors:Bayabil; Haimanote K., Teshome, Fitsum T., Hailegnaw, Niguss Solomon, Zhang, Jian, Li, Yuncong C., Adamczyk, Bartosz First page: 27 Abstract: The effect of milorganite, a commercially available organic soil amendment, on soil nutrients, plant growth, and yield has been investigated. However, its effect on soil hydraulic properties remains less understood. Therefore, this study aimed to investigate the effect of milorganite amendment on soil evaporation, moisture retention, hydraulic conductivity, and electrical conductivity of a Krome soil. A column experiment was conducted with two milorganite application rates (15 and 30% v/v) and a non-amended control soil. The results revealed that milorganite reduced evaporation rates and the length of Stage I of the evaporation process compared with the control. Moreover, milorganite increased moisture retention at saturation and permanent wilting point while decreasing soil hydraulic conductivity. In addition, milorganite increased soil electrical conductivity. Overall, milorganite resulted in increased soil moisture retention; however, moisture in the soil may not be readily available for plants due to increased soil salinity. PubDate: 2022-11-28 DOI: 10.1017/exp.2022.25
Authors:Newall; Philip W. S., Ferreira, Catia Alexandra, Sharman, Steve, Payne, Jessica First page: 28 Abstract: Gambling marketing is frequently visible in the United Kingdom, especially around the national sport, soccer. Previous research has documented the frequency with which gambling marketing logos can be seen in domestic club soccer, and also the frequency of television advertising around international tournaments. The present research investigates the frequency and content of television advertising during the men’s 2020 Euro soccer tournament, a high-profile tournament shown since the industry’s voluntary “whistle-to-whistle ban” on gambling advertising came into effect. Overall, 113 gambling adverts were recorded (4.5 adverts per relevant match). Financial inducements were the most frequently shown category (56.6%), followed by adverts raising awareness of a given operator’s brand (19.5%), adverts featuring the odds on specific complex bets (18.6%), and adverts promoting safer gambling (5.3%). Adverts featured a range of safer gambling messages, with the “when the fun stops, stop” message featuring in 56.6% of adverts. This research indicates that gambling advertising remains a frequent part of the experience of watching live televised soccer in the UK, and shows how the content of this advertising was comparable to what has been seen in the previous literature. PubDate: 2022-12-09 DOI: 10.1017/exp.2022.26