Authors:AISAR NOVITA, WILLY EKA PRASETYA, WAN ARFIANI BARUS Pages: 130 - 134 Abstract: Root induction is an important step in plant propagation in vitro. Root stimulation can occur in the presence of the hormones auxin and gibberellins which can be obtained from other plants, one of which is bananas. The research aims to determine the effect of various species and concentrations of the banana extract on Phalaenopsis amabilis root induction in vitro. The research was conducted in the Laboratory of Tissue Culture Alifa Agricultural Research Center (AARC), Medan, North Sumatera, from May until July 2020. The research used a Factorial Completely Randomized Design (CRD) with 2 factors, the first factor was the type of banana extract with 3 levels, consisting of Ambon, Raja, and Kepok. The second factor is the concentration of banana extract (C) with 4 levels, consisting of 50 g/l, 100 g/l, 150 g/l, 200 g/l. The results showed that the concentration of banana extract had a significant effect on the number of roots, but various species of bananas and the interaction of the species of bananas and concentration banana extract had no significant effect on root induction PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.25261 Issue No:Vol. 22, No. 3 (2022)
Authors:ZUSFAHAIR ZUSFAHAIR, DIAN RIANA NINGSIH, AMIN FATONI, ELY SETIAWAN Pages: 135 - 140 Abstract: Urease is an enzyme that functions as a catalyst in the hydrolysis of urea into ammonia and carbon dioxide. The industrial sector has made extensive use of urease. To date, enzymes are used in free form, deemed less effective. Therefore, enzymes are used in immobilized form because they can be utilized repeatedly. This research aimed to isolate urease from kidney bean (Phaseolus vulgaris L.) seed and immobilize it using a Ca-alginate support matrix and a trapping technique. Eight days were devoted to germinating kidney bean seeds to begin the investigation. Isolation of crude urease extract from kidney beans was carried out using phosphate buffer pH 7. It was then immobilized with Ca-alginate at different concentrations of Na-alginate and contact times The crude free and immobilized urease extract was further characterized including pH, temperature and stability of repeated use. The urease activity was determined using the Nessler method using a spectrophotometer. The results demonstrated that urease immobilization from kidney bean seeds with a Ca-alginate matrix was most effective at a concentration of 5% Na-alginate and a contact period of 60 minutes, yielding a value of 5.92 U/mL. The optimal pH of free and immobilized urease was 7 and 8, respectively, and temperatures of 35 and 40 °C, respectively. The immobilization of urease from kidney bean seeds using a Ca-alginate support matrix increased the stability of recurrent use by fivefold, while the relative urease activity remained at 52%. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.26056 Issue No:Vol. 22, No. 3 (2022)
Authors:SADLI SADLI, SITTI SALEHA, RAIYAN RAIYAN Pages: 141 - 147 Abstract: Mushrooms contain a substance known as glutamic acid, that produce a savory taste which has the potential to be developed into a natural flavoring. The research began with the determination of amino acid levels in three phases of harvesting straw mushrooms, namely: the egg phase, the bud phase and the adult phase, by using High Performance Liquid Chromatography which aims to determine the harvesting phase with the highest glutamic acid content. The test results of amino acid levels showed the adult phase contained the highest glutamic acid levels of 49.08 mg/g. Furthermore, the manufacture of straw mushroom flour was performed with temperature variations of 60° C and 70° C and drying times of 6, 7 and 8 hours. Then the flavoring formulation was completed by mixing mushroom flour and other dry additives namely shallots, garlic, white pepper, tapioca flour, salt and sugar. The resulting flavoring test is conducted using a description test by assessing the sensory properties of natural flavorings, namely color, aroma, taste and texture. Based on a panelist rating, F2 (60°C, 7 hours) has the best score for color (3.88) and aroma (3.32). F5 (70°C, 7 hours) has the best score for taste (3.24) and F1 (60°C, 6 hours) has the best score for texture (2.08). The conclusion of this study is that mushrooms have a very high potential to be deformalized into natural flavorings, as well as the natural flavoring quality parameters of mushrooms is influenced significantly by temperature parameters and drying time PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.22885 Issue No:Vol. 22, No. 3 (2022)
Authors:CUT MAILA HANUM, MA’RIFATIN ZAHRAH, MALAHAYATI DAUD, ROZZA TRI KWATRINA Pages: 148 - 155 Abstract: Hunting-park is a conservation area with the unique attraction of periodically hunting in terms of wildlife management. Lingga Isaq Hunting-Park (LIHP) is one of 13 hunting parks in Indonesia. However, the utilization of LIHP as a hunting park is still facing obstacles; two of which are conflicts of interest among authorities and no data of the animals, especially mammals – the most hunted creatures in the park. Therefore, a study was conducted to identify the mammalian abundance and distribution. This data would be usefull as essential decision-making for hunting-tourist attractions in LIHP. This study used the transect method, with 12 transect strips evenly distributed in those four function areas; three per each. We found 11 species of mammalian that belonging to 8 families. The protected forest had the highest biodiversity value (H’=1.50), while other use areas had the lowest biodiversity value (H’=1.16). Macaca fascicularis had the highest abundance (23 individu), while Sus scrofa was the most evenly distributed mammal in the whole landscape. Protected forests and other utilization areas have the highest habitat similarity, identified as having intense anthropogenic activities. The protected forest also has habitat similarity with hunting-park as both areas are legally protected. Nevertheless, there are only Cervus unicolor, Muntiacus muntjak, and Sus scrofa that are highly recommended for hunting tourism attractions. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.27067 Issue No:Vol. 22, No. 3 (2022)
Authors:SUSWATI SUSWATI, SERI DEPI, SAISA SAISA, SITI MARDIANA, SAIPUL SIHOTANG Pages: 156 - 167 Abstract: Factors that cause fluctuations in chili production are nutrient content and attack by plant disturbing organisms (PDO). To overcome pest attacks can be done by planting refugia which serves as an alternative that will bring in natural enemies. In addition, the application of cow dung and mycorrhizal compost as biological agents will be able to maintain soil quality and plant growth. This study aims to determine the effect of cow waste compost and arbuscular mycorrhizal fungi (AMF) on the growth and production of red chili plants intercropped with refugia plants. The method was a factorial randomized block design consisting of 2 factors, namely: cow waste compost and AMF. Cow waste compost consists of 5 levels, namely: K0 = without giving cow waste compost, K1 = cow waste compost at a dose of 5 tons/ha-1, K2 = cow waste compost at a dose of 10 tons/ha-1, K3 = cow waste compost at a dose 15 tons/ha-1, K4= cow waste compost dose of 20 tons/ha-1. Meanwhile, arbuscular mycorrhizal fungi consisted of 4 levels, namely: M0= without AMF, M1= AMF 50 kg/ha-1, M2= AMF 100 kg/ha-1, M3= AMF 150 kg/ha-1. The results showed that AMF had a most significant effect on the number of fruits per plant, production per plant, and production per plot, but not significantly different on plant height, some branches, and flowering age of Laris variety red chili. AMF treatment with 150 kg/ha (M3) dose had the highest average value for all observed parameters. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.25530 Issue No:Vol. 22, No. 3 (2022)
Authors:ESSY HARNELLY, NITA TAUHIDA, ISKANDAR ZULKARNAIN SIREGAR Pages: 168 - 172 Abstract: DNA extraction is the first step in obtaining high-concentration and high-purity DNA that can use in the subsequent steps. Because the leaf structure is challenging and contains several secondary metabolites that can affect DNA extraction results, DNA extraction on dry leaves of Dipterocarpaceae is considered problematic. This research aims to find a suitable method for extracting DNA from the preserved Dipterocarpaceae leaves. Preserved leaves of Dipterocarpaceae in silica gel will dry up, making them tough to destroy. Perform DNA extraction is generally using CTAB buffers or extraction kits. According to the results of this study, extraction with modified CTAB buffer result in DNA with higher concentration and purity values than DNA extracted with the kit. DNA isolated from the modified CTAB can be used as a template in the PCR process to amplify the matK gene. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.26588 Issue No:Vol. 22, No. 3 (2022)
Authors:RESTI PUSPA KARTIKA SARI, NANIK SRIYANI, YUSNITA YUSNITA, HIDAYAT PUJISISWANTO Pages: 173 - 181 Abstract: Diuron herbicide has been used in the pineapple plantation in Lampung, Indonesia, for more than 35 years. It has been realized that the use of herbicides with the same mode of action intensively can speed up the evolution of resistant weeds over a long period of time. This study aimed to determine whether Dactyloctenium aegyptium from pineapple plantation has evolved resistance to diuron and to examine whether the resistance correlates with the weed physiological activities. The study was conducted at the University of Lampung, from September 2018 to March 2019. The study consisted of two stages, i.e. Stage 1: Weed resistance test and Stage 2: Physiological activity test on resistant weed. The study used a split-plot design. The main plot was the origins of weeds (exposed and unexposed to diuron) and thhe supplots was the diuron dose. The result showed that D. aegyptium exposed has high-level resistance to diuron. The physiological activities of D. aegyptium which has a high level of diuron resistance exhibited higher carbon assimilation, stomatal conductance, and transpiration rates than the sensitive D. aegyptium. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.25286 Issue No:Vol. 22, No. 3 (2022)
Authors:RITA HAYATI, AINUN MARLIAH, RIFA NARIZKY Pages: 182 - 197 Abstract: Chrysanthemum morifolium L. is one of the ornamental plants that has problems in maintaining the freshness of the flower. The handling given to maintain the shelf life of the flower is to soak the flowers with a natural preservative solution. This study aims to find out the effect of several types of natural preservative solutions and long soaking on the quality of freshness of chrysanthemum cut flowers, as well as to find out whether there is an interaction between the two treatments. This research was conducted at the Laboratory of Seed Science and Technology and Horticulture Laboratory of the Department of Agrotechnology, Faculty of Agriculture, University of Syiah Kuala, Banda Aceh from March 30 to April 15, 2021. The study used a Complete Randomized Design of a 4 x 3 Factorial pattern with 3 repeats. The parameters observed include the diameter of the full blooming flower, the flower's ness index, the flower freshness period index, total absorbed solution, color measurement and organoleptic test. The results showed that the combination of treatment between natural and long-soaked solution types had a real effect on the parameters of the flower freshness index. The best freshness period of flowers is in the combination of 10% sugar solution type treatment with a 6-hour soaking length. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.25354 Issue No:Vol. 22, No. 3 (2022)
Authors:FAHRAUK FARAMAYUDA, RENALDI OKTAVIANUS, ELFAHMI ELFAHMI Pages: 198 - 208 Abstract: Stevia rebaudiana is very famous as natural sweeteners and potentially for lowering blood sugar. The benefits of Stevia rebaudiana are numerous, and various pharmacological activities have been found, such as efforts to treat diseases such as diabetes, obesity, dental caries, hypoglycemia, and hypertension. The main sweet compounds in Stevia rebaudiana are rebaudioside A and stevioside, especially in the leaves. This review examines aspects of phytochemistry, pharmacological activity, micropropagation, and in-vitro culture modification of Stevia rebaudiana plants. Online and offline literature searches were conducted to compile the articles. PubMed (Medline), Web of Science, is used to retrieve online publications using the following search terms: Stevia rebaudiana, stevia herbs, traditional medicine, pharmacological activities, toxicity, secondary metabolite content, phytochemicals, and plant tissue culture of Stevia rebaudiana . This review is also a compilation of four previous reviews and is accompanied by the latest data from research journals on Stevia rebaudiana. PubDate: 2022-10-31 DOI: 10.24815/jn.v22i3.19477 Issue No:Vol. 22, No. 3 (2022)
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