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Majalah Kedokteran Gigi Indonesia
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This is an Open Access Journal

Open Access journal
ISSN (Print) 2460-0164 - ISSN (Online) 2442-2576
Published by Universitas Gadjah Mada

[48 journals]

Effect of low-intensity pulsed ultrasound on mast cell degranulation and
fibroblast expression on type 2 diabetes mellitus rats wound healing
process
- Authors: Intan Maharani, Cahya Yustisia Hasan, Bambang Dwirahardjo, Tri Wahyu Pangestiningsih
  Pages: 60 - 68
  Abstract: Impaired wound healing is one of the Diabetes mellitus complications. Low-intensity Pulsed Ultrasound (LIPUS) therapy may accelerate the impaired wound healing. The use of LIPUS therapy in the early inflammatory phase can induce mast cell degranulation, and in the proliferative phase it can increase collagen synthesis by fibroblasts. The purpose of this study was to determine the effects of LIPUS therapy on mast cell degranulation and fibroblast
  expression in the healing process of punch biopsy wound in rats with type 2 diabetes mellitus. Twenty-four Sprague dawley (n=24) were designed into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then divided into six groups: diabetes mellitus without LIPUS (DM3, DM7, DM14) and diabetes mellitus with LIPUS (DML3, DML7, DML14), 4 each, and punch biopsy wounds were made on the dorsal skin. The DML group received LIPUS therapy in the wound area (frequency 3 MHz, intensity 0.5 W/cm2, duty cycle 20%, duration 3 minutes every day for 3 days (DML3), 7 days (DML7), and 14 days (DML14). The wounded tissue area was stained with toluidine blue to observe mast cell degranulation and immunohistochemical type HSP-47 to observe fibroblast expression. Two-Way ANOVA and Post Hoc LSD tests were used to determine the differences in mast cell degranulation and fibroblast expression. The results showed that mast cell degranulation and fibroblast expression in the DML group were higher than in the DM group (table 1). Pearson test showed a correlation between mast cell degranulation and fibroblast expression (p=0.00; r= 0.839). LIPUS therapy increases mast cell degranulation and fibroblast expression in type 2 diabetes mellitus rat model. The higher the mast cell degranulation, the higher fibroblast expressions.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.59132
  Issue No: Vol. 7, No. 2 (2022)
In-vitro cytotoxicity activity of potato (Solanum tuberosum. L) peel
extracts against human gingival fibroblasts
- Authors: Khong Mei Xuan, Anne Handrini Dewi, Ivan Arie Wahyudi
  Pages: 69 - 75
  Abstract: Potato peel is often regarded as waste although it contains phenolic compounds, glycoalkaloids, and flavonoid. This study aimed to evaluate the effect of different concentrations of potato peel extracts on the viability of Human Gingival Fibroblasts (HGF). Potato peel extracts were prepared by a maceration technique. The 96-well tissue culture micro titre plates were seeded with HGF at a density of 2×104 cells/100 μL and incubated for 24 hours. Next, 100 μL of potato peel extracts at a concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL and a medium (control) were dispensed into the well of the cell culture. Each concentration was evaluated for its viability with 3 replicate samples. The results of the MTT test were analyzed statistically using one-way ANOVA and LSD test. The mean and standard deviation of the viable HGF after incubated with the potato peel extract at the concentration of 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL were 98.67% ± 3.56, 88.34% ± 0.79, 55.42% ± 3.96, 28.33% ± 0.60, and 26.26% ± 0.53, respectively. The percentage of non-viable HGF increased with an increase in the concentration of the potato peel extract. The ANOVA test result showed a significant influence of various concentrations of the potato peel extract on the viability of HGF (p<0.05). The result of the LSD-test showed a significant difference among all the treatment groups (p<0.05). A higher concentration of potato peel extracts increased the viability of HGF cell line and the concentrations of 62.5 μg/mL and 125 μg/mL were considered non-cytotoxic.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.40196
  Issue No: Vol. 7, No. 2 (2022)
Educational approaches to improving knowledge and attitude towards dental
hygiene among elementary school children
- Authors: Nenny Wuri Prabawati, Lisdrianto Hanindriyo, Sri Widiati
  Pages: 76 - 83
  Abstract: The selection of appropriate dental health education methods will be beneficial in promoting dental health. This study aimed to determine the difference in the effect of role-playing method and storytelling method on knowledge and attitudes towards oral hygiene among elementary school students. The research subjects were 112 students in grade 5. The subjects were divided into 2 different treatment groups, namely 56 students in grade 5 at SD Negeri Tegalrejo I with the storytelling method and 56 students in grade 5 at SD Negeri Tegalrejo II using the role-playing method. The measuring instrument in this research was a questionnaire. The data analysis used the Mann-Whitney test and Wilcoxon Signed Ranks test because the data were not normally distributed. The results of the analysis showed that there was a significant increase over time in knowledge and attitudes carried out in 3 assessments. The mean rank for delta values between the pre-test and posttest 2 for the knowledge variable using the role-playing method was 51.29 while that using the storytelling method was 61.71. Meanwhile, the mean rank for delta values for the attitude variable using the role-playing method was 49.93, while that using the storytelling method was 63.07. The results of the delta analysis from pre-test to post-test 1 and pre-test to post-test 2 showed that the storytelling group experiences a higher increase in knowledge and attitudes than the role-playing group (p<0.05). Provision of education using a storytelling method shows better improvement in students’ knowledge and attitudes towards oral hygiene than using a role-playing method.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.60833
  Issue No: Vol. 7, No. 2 (2022)
Bacterial adhesion of Streptococcus mutans to cobalt chromium recast
alloys
- Authors: Dewi Arsih Sulistiani, Widjijono Widjijono, Rini Dharmastiti
  Pages: 84 - 87
  Abstract: Cobalt chromium (CoCr) alloys are utilized to make dental prosthesis. Casting CoCr alloys is a common operation in dentistry laboratories due to its low cost. Casting surplus (metal remaining in the sprue and crucible former) is frequently reused by dental laboratories to reduce and recycle metal waste. However, the quality and safety of these recast alloys require further information. Microbial attachment to the surface of metal prostheses may affect its quality and safety. Biofilm formation on metal surface can cause biocorrosion and secondary infection. The effects of different proportions of recast Cobalt Chromium alloy on the bacterial adhesion are not clear. The purpose of this study was to investigate how recasting affects the Streptococcus mutans (S. mutans) adhesion. Twenty disk-shaped specimens (n= 20, allocated for 5 groups) were prepared from CoCr alloys (Remanium GM; Dentaurum) with different proportions (100% new alloy, 25% recast alloy, 50% recast alloy, 75% recast alloy, and 100% recast alloy). After the immersion of the specimens in bacterial suspension for 24 hours, the number of bacteria that adhere to the specimen’s surface was counted using Colony Forming Units. Data were analyzed with one-way ANOVA (α = 0.05). The bacterial adhesion was significantly affected by the recast alloys (p < 0.05). An increased proportion of CoCr recast alloys showed an increase in S. mutans adhesion to the specimen surface.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.53329
  Issue No: Vol. 7, No. 2 (2022)
Effect of two different materials in sealing vertical root fractures of
intentional replantation on epithelial thickness of periradicular tissue
- Authors: Raphael Tri Endra Untara, Widjijono Widjijono, Widya Asmara, Diatri Nari Ratih
  Pages: 88 - 94
  Abstract: Vertical root fractures of intentional replantation need a material capable of binding the fragments of the fracture line tightly and encouraging the regeneration of periradicular tissue. One of the indicators that regeneration of periradicular tissue takes place is epithelial thickness. This study aimed to investigate the effect of mineral trioxide aggregate (MTA) and self-adhesive resin cement as the adhesive materials in sealing vertical root fractures of intentional replantation on the epithelial thickness of periradicular tissue. This study used 27 male New Zealand rabbits with the age range of 8-12 weeks. The mandibular incisor was extracted, and the tooth was cut from the cervical border to the 2/3 apical third. The samples were assigned randomly into three groups of 9 each, namely Group 1 with no application of any material in the fracture line (control group), Group 2 with MTA, and Group 3 with self-adhesive resin cement. All the teeth in all the groups were then inserted back into the socket. Each group was further divided into three subgroups based on the observation time, namely days 7, 14, and 21. Histological observations of the epithelial thickness were carried out under the light microscope (400x magnification). Data were analyzed using a two-way ANOVA and LSD post hoc test with a confidence level of 95%. The two-way ANOVA analysis showed that the materials used for sealing the fracture lines had a significant effect on the epithelial thickness (p<0.05), while the observation time did not affect the epithelial thickness (p>0.05). No interaction occurred between the material used and observation times (p>0.05). It can be concluded that MTA generated a greater epithelial thickness of periradicular tissue compared than self-adhesive resin cement in sealing vertical root fractures of intentional replantation.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.66092
  Issue No: Vol. 7, No. 2 (2022)
Comparison of apical sealing ability between bioceramic and zinc oxide
eugenol-based sealer during root canal treatment, in vitro
- Authors: Dedy Agoes Mahendra, Yora Nindita, Gustantyo Wahyu Wibowo, Gloria Fortuna
  Pages: 95 - 101
  Abstract: Obturation with a sealer material that provides an adequate apical sealing ability is required to prevent endodontic treatment failure due to microleakage. However, there are no sealers that meet all the physical and chemical properties to be able to hermetically seal the root canal system to date. Various sealer materials have been developed in recent years including the use of bioceramic materials which are claimed to have excellent biocompatibility to tissues. This study aimed to compare the apical sealing ability of bioceramic-based and zinc oxide eugenol (ZOE)-based sealer in root canal treatment. A total of 27 extracted mandibular premolars were decoronated to the standard root length of 14 mm. The root canals were prepared with a crown-down technique using manual instrument to file F3 (30/.09). The samples were then divided into three groups: obturation with bioceramic-based sealer (n=9); ZOE-based sealer (n=9); and control group (n=9). Microleakage was measured using a dye penetration method with 1% methylene blue and observed under stereomicroscope at x20 magnification. The mean of the maximum penetration length from the lowest to the highest was found in the bioceramic-based sealer group (0.825 mm), the ZOE-based sealer group (3.850 mm), and the control group (4.444 mm). One-way ANOVA test showed a significant difference in the maximum penetration length between the three groups (p<0.05). The post hoc LSD test showed a significant difference in the maximum penetration length between the bioceramic-based and ZOE-based sealer groups (p<0.001). Obturation with bioceramic-based sealer provides a better apical sealing ability than that with ZOE-based sealer.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.62212
  Issue No: Vol. 7, No. 2 (2022)
Effect of Nanofilled Resin-Based Coating on the Compressive Strength of
Glass Ionomer Cement – in vitro
- Authors: Ardy Setiawan, Juanita Amaludin Gunawan, Selviana Wulansari, Didi Nugroho
  Pages: 102 - 109
  Abstract: Glass ionomer cement as one of the restoration materials requires high compressive strength so it can last during functional activity. The latest glass ionomer cement comes with glass hybrid technology and a nanofilled resin-based protective coating which is said to increase the compressive strength of glass ionomer cement. The aim of this study was to analyze the effect of nanofilled resin-based coating and the types of glass ionomer cement materials on their compressive strength. Two types of commercial glass ionomer cement material were used; conventional (Fuji IX GP Extra), and hybrid (EQUIA Forte Fill) glass ionomer cement. Forty cylindrical (4 x 6 mm) samples were prepared in each group. The main group was divided into 4 subgroups (n=10) based on the protective coating used (EQUIA Forte Coat, Varnish, Control, Water + EQUIA Forte Coat). Eight subgroups were immersed in 37 °C distilled water for 7 days, then a compressive strength test was performed using a universal testing machine. The data analysis showed no significant difference in the compressive strength between the two types of glass ionomer cement materials (p>0.05). The use of a protective coating was associated with a significant decrease in the compressive strength (p<0.05). The use of glass ionomer cement without the application of a protective coating was considered to be quite good because the compressive strength value of the restoration still met the standards of the American Dental Association.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.65512
  Issue No: Vol. 7, No. 2 (2022)
Effect of advanced-platelet rich-fibrin combined with rosuvastatin
application after open flap debridement of infrabony pocket
- Authors: Tissa Rahadianti, Dahlia Herawati, Kwartarini Murdiastuti
  Pages: 110 - 117
  Abstract: Open flap debridement (OFD) is an invasive therapy for chronic periodontitis with pocket 5 mm or more. However, it is difficult to achieve regeneration and new attachment with this therapy. Periodontitis starts to add growth factors and local drugs delivery as host modulation therapy. Advanced-PRF (A-PRF) contains more growth factor than PRF which plays a role in promoting fibroblast proliferation, reepithelization, extracellular matrix production, and endothelial cell migration. 1.2% rosuvastatin gel (RSV) is a local delivery drug with a pleiotropic effect that can modify host response to promoting BMSCs, BMP-2, OPG, ALP, RANKL, and osteoblasts. This study aimed to examine the effect of the application of A-PRF+RSV in OFD therapy of which the parameters were probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The study samples consisted of 24 periodontal pockets which were divided into 2 groups of 12 pockets each, namely A-PRF+RSV for group 1 and PRF+RSV for group 2. Clinical evaluations were carried out on baseline, day-30, and day-90 for PD and RAL, and on baseline and day -90 for alveolar bone height. Data of PD and RAL reduction were analyzed with non-parametric test Mann-Withney, while data of reduction of alveolar bone height were analyzed with parametric Independent-T test. Group 1 obtained a statistically more significant result in reducing PD, RAL, and alveolar bone height compared to group 2 (p<0.05) To conclude, the application of A-PRF and 1.2% rosuvastatin gel in OFD procedure promotes a higher PD and RAL reduction and alveolar bone height increase than the application of PRF coupled with 1.2% rosuvastatin gel.
  PubDate: 2022-06-07
  DOI: 10.22146/majkedgiind.53419
  Issue No: Vol. 7, No. 2 (2022)