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Brazilian Dental Science
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ISSN (Print) 1678-2046 - ISSN (Online) 2178-6011
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Effect of whitening mouthrinses on color change of stained resin
composites
- Authors: Mariane Cintra Mailart, Érica Crastechini, Juliana Boa Sorte Oliveira, Carlos Rocha Gomes Torres, Taciana Marco Ferraz Caneppele, Alessandra Buhler Borges
  Abstract: Objective: The present study compared the effect of whitening mouthrinses (WM) on the color change of stained
  resin composites (RC). Material and Methods: Cylindrical specimens (6mm-diameter and 1mm-thickness)
  were prepared with the following RC (n=60/group): Filtek Z350XT (Z350- methacrylate-based), Admira Fusion
  (AD- ormocer-based), TPH3 (TPH- methacrylate-based), and Beautifil II (BII- giomer/methacrylate-based).
  The initial color was assessed with reflectance spectrophotometer using CIE L*a*b* system. The specimens
  were immersed in staining broth during 14 days, submitted to color evaluation (E1) and randomly allocated
  in 4 subgroups (n=15), according to WM adopted: Listerine Whitening (LW-2% hydrogen peroxide), Plax
  Whitening (PW-1.5% hydrogen peroxide), Bromelain/papain (BP-experimental solution), and Deionized water
  (DW-negative control). The whitening cycle consisted of RC immersion in WM for 1 min and in artificial saliva
  for 30 min, simulating 12 weeks, and final color assessment was performed (E2). Color change data were
  analysed by ANOVA and Tukey’s tests (a=5%). Results: After staining, TPH showed the lowest E1 values
  and Z350 showed the highest color change (p=0.001). The whitening effect promoted by LW was significantly
  higher than color alteration obtained with PW (E2), and BII showed the highest color change values (E2) after
  whitening cycle. Conclusion: LW exhibited the greatest whitening potential on stained RC, mainly with the
  Giomer (Beautifill II) and the Ormocer-based (Admira Fusion) materials. Bromelain/papain solution showed
  no whitening effect on stained RC.
  KEYWORDS
  Giomer; Hydrogen peroxide; Ormocer; Resin composite; Whitening mouthrinses.
  PubDate: 2022-02-04
  DOI: 10.4322/bds.2022.e2773
  Issue No: Vol. 25, No. 1 (2022)
Force decay and discoloration of thermoplastic and thermoset orthodontic
elastomeric chains
- Authors: Saeid Foroughi Moghaddam, Mohammad Damavandi Kamali
  Abstract: Objective: Elastomeric chains are commonly used to apply force for orthodontic dental movements. However,
  force decay and discoloration are two important weak points of these materials. The present study intended to
  compare the force decay and color stability of different types of elastomeric chains. Material and Methods: This
  in vitro study evaluated 6 groups of elastomeric chains, including thermoplastic (TP) and thermoset (TS) chains
  made by the companies American Orthodontics (AO), Ormco (OR), and G&H Orthodontics (GH). The elastomeric
  chain forces were measured at the baseline, following 1 hour, 1 day, 1, 2, 3, 4, 5, and 6 weeks of stretching.
  The elongation required for the chains to exert a force of 250 g was calculated. E of each group was calculated
  by a spectrophotometer following immersion in black tea solution for 6 days. Data were analyzed using the
  SPSS 22 software and the statistical methods of repeated measures analysis of variance and two-way analysis of
  variance (P<0.05). Results: The force decay of the TS chains were significantly lower than the TP chains in the
  6-week study duration (P<0.05), and the lowest and highest force decay was observed in the products by AO
  and GH, respectively. The highest force degradation occurred during the first week in all groups. The elongation
  rate needed for the TS chains was significantly higher than the TP chains (P<0.05), and the highest elongation
  rate was observed in the products by OR. TS chains showed significantly higher color stability than TP chains,
  and products by OR and GH had better color stability than the products by AO. Conclusion: The present study
  showed that TS chains were superior to TP chains in force decay and color stability in all the brands studied.
  KEYWORDS
  Color; Elastomeric; Force decay; Thermoplastic; Thermoset.
  PubDate: 2022-02-04
  DOI: 10.4322/bds.2022.e2612
  Issue No: Vol. 25, No. 1 (2022)
Interaction of dental anxiety and ABO secretor status of blood group on
dental caries in a group of children
- Authors: Zainab Jafar
  Abstract: Objective: To evaluate the interaction of the dental anxiety precedents at the first dental examination and
  the AB secretor status of blood groups (A, B, AB, and O) on dental caries experience and severity in a group
  of school children. Material and Methods: This is a cross-sectional study included 81 healthy children aged
  6-8 years from primary schools in Baghdad; with negative previous dental visit. Dental anxiety represented by
  physiological measures was measured by pulse oximeter. Dental caries experience and severity was recorded.
  Unstimulated salivary samples were taken from the children to determine the salivary secretor status for AB
  blood type. Results: The anxiety was decreased as age increases; boys were more anxious than girls; and more
  anxious children were found in non-secretor group; Dental caries was higher in non-secretor anxious children
  all with non-significant difference. Conclusion: There is little evidence that the AB secretor status and anxiety
  to have influence on dental caries experience and severity, as there are many factors may interact to propagate
  the caries experience and severity.
  KEYWORDS
  Dental anxiety; Dental caries; Children.
  PubDate: 2022-02-03
  DOI: 10.4322/bds.2022.e2928
  Issue No: Vol. 25, No. 1 (2022)
A study on the correlation between smoking and non-enzymatic antioxidant
factors of the saliva of healthy smokers and non-smokers
- Authors: Mahdieh Zarabadipour, Seyed Amir Hadi Hosseini, Hashem Haghdoost-Yazdi, Ehsan Aali, Pedram Yusefi, Monirsadat Mirzadeh, Hossein Piri
  Abstract: Objective: Smoking is among the most destructive habit which has numerous effects on the body. The chemical competent of cigarettes destroy the anti-oxidant content of the saliva. In this study, the concentration of albumin and uric acid of smokers was measured based on the frequency of smoking. Material and Methods: In this cross-sectional study, 26 heavy smokers, 27 moderate smokers and 29 non-smokers between the ages of 25 to 40 were selected. The subjects did not suffer from any systemic or periodontal conditions. Unstimulated saliva was collected by spitting. The amount of albumin was measured by Bromocresol Green, and the amount of uric acid was measured by the uricase method. The selected method of analysis, using SPSS software, was One-Way ANOVA. Results: Mean albumin content of saliva was 10.22 ± 2.10 mg/dl in non-smokers and 23.88 ± 8.93 mg/dl in heavy smokers. Both uric acid and albumin content was significant in heavy smokers and non-smokers (P=0.001). Conclusions: Based on the findings of this study, saliva concentrations of both Albumin and Uric Acid change based on the frequency of smoking. Decrease of saliva albumin and an increase of uric acid is an indicator of the unhealthy effects of smoking on the body. KEYWORDS
  Uric Acid; Albumin; Saliva; Cigarette Smoking; Oxidative stress.
  PubDate: 2022-02-03
  DOI: 10.4322/bds.2022.e2867
  Issue No: Vol. 25, No. 1 (2022)
A study comparing patient satisfaction and retention of CAD/CAM milled
complete dentures and 3D printed CAD/CAM complete dentures versus
conventional complete dentures: a randomized clinical trial
- Authors: Mohamed Mohamed Abo Heikal, Nouran Abdel Nabi , Maha Wagdy Elkerdawy
  Abstract: Objective: To compare the three different methods of complete denture fabrication assessing patient satisfaction
  and retention after insertion for six months’ follow-up period. Material and Methods: The study was conducted
  in the Prosthodontic Department where a total of forty-eight patients were recruited from the outpatient clinics
  fulfilling the inclusion criteria. This study was designed as a randomized controlled clinical trial. All patients
  followed the steps of complete denture construction till jaw relation record. Then, all eligible patients were
  randomized to intervention and control groups. For intervention groups digital scanning, designing, manufacturing
  of complete dentures was done; 3D printing for first and milling for second intervention. For the comparator
  group, complete dentures were manufactured the conventional way. After 2 weeks of delivery of the dentures,
  patients received a patient satisfaction questionnaire, retention was measured by retention force gauge. Both
  readings were also recorded after 3 months and at 6 months. The mean and standard deviation values were
  calculated for each group in each test. The significance level was set at P < 0.05. Results: No statistical difference
  was found in terms of patient satisfaction and retention between the three groups at different time intervals.
  Conclusion: The manufacturing technique seemed to have no influence on patient satisfaction and retention
  with milled showing the least results.
  KEYWORDS
  Computer-aided design; Denture, Complete; Denture retention; Patient satisfaction; Printing, Three-dimensional
  PubDate: 2022-02-03
  DOI: 10.4322/bds.2022.e2785
  Issue No: Vol. 25, No. 1 (2022)
Effect of laser-assisted retrograde cavity preparation on
push-out-bond-strength of mineral trioxide aggregate and
calcium-enriched-mixture-cement
- Authors: Hasibe Sevilay Bahadır, Ali Türkyilmaz, Yusuf Bayraktar, Çiğdem Çelik
  Abstract: Objective: This study aimed to determine the push-out-bond-strength(PBS) of mineral trioxide aggregate
  (BIOMTA+) and calcium-enriched-mixture-cement (CEM) in retrograde cavities prepared using Er: YAG laser
  and stainless-steel bur. Material and Methods: The root canals of 60 extracted single-rooted human teeth were
  prepared, filled and their apical portion of 3-mm were resected using a diamond bur and randomly divided
  into four groups according to technique of retrograde preparation and filling material as follows(n=15):
  Group1: bur/ BIOMTA+ ,Group2: bur/ CEM, Group3: Er:YAG laser/ BIOMTA+, Group4: Er:YAG laser/ CEM.
  PBS test were performed to specimens and failure modes were evaluated. The data were statistically analyzed
  with ANOVA, Post-Hoc Tukey and t tests (p< 0.05). Results: CEM was exhibited higher than bond strength
  compared to BIOMTA+ in retrograde cavity prepared using laser (p= 0.021) and BIOMTA+ in retrograde cavity
  prepared using bur was exhibited higher than bond strength compared to in retrograde cavities prepared using
  laser (p= 0.024). Failure modes were dominantly cohesive in all groups tested and one representative specimen
  each failure mode was examined in SEM and the general characteristics of the failure modes were confirmed.
  Conclusion: With in the limitations of the present study, when used CEM, Er: YAG laser-assisted retrograde
  cavity preparation positively affected the bond strength values compared to BIO MTA+. Considering its optimal
  adhesion, the calcium-enriched-mixture-cement (CEM) might be a good option as a filling material in retrograde
  cavities in clinical use.
  KEYWORDS
  Calcium-Enriched-Mixture-Cement (CEM); Er: YAG laser; Mineral trioxide aggregate; Retrograde cavity preparation.
  PubDate: 2022-02-02
  DOI: 10.4322/bds.2022.e2736
  Issue No: Vol. 25, No. 1 (2022)
Frequency of oral Lichen Planus among hypothyroid patients
- Authors: Maryam Baharvand, Farzaneh Hamrahi , Shahzad Gholami, Parisa Hajighasem
  Abstract: Objective: Recent investigations have suggested a correlation between thyroid diseases, particularly hypothyroidism
  (HT), and oral lichen planus (OLP). Objective: This study aimed to assess the frequency of OLP in HT patients.
  Material and Methods: This analytical descriptive study evaluated 100 HT patients including 94 females and
  6 males, who were selected by convenience sampling. The subjects were clinically examined for OLP. The mean
  age of patients was 42.49±1.29 years. Also, 100 age- and sex-matched healthy controls were examined. The
  data were analyzed using chi-square test and Fisher’s exact test via SPSS. Results: Of all, 14 (14%) HT patients
  (12 females and 2 males) had OLP while only one case of OLP was found in the control group. The difference
  between the two groups in the frequency of OLP was significant (P=0.001). The odds ratio (OR) of developing
  OLP was calculated to be 16.11 in HT patients. Reticular form was the most common type of OLP in patients
  (50%). The buccal mucosa was the most common site of involvement (92.85%). The mean age of patients with
  OLP was 42.93±1.29 years with a median of 46±1.29 years in HT group. Conclusion: The frequency of OLP
  was significantly higher in HT patients such that HT patients had higher odds of developing OLP by 16 folds.
  KEYWORDS
  Hypothyroidism; Oral lichen planus; Frequency; Comparison.
  PubDate: 2022-02-02
  DOI: 10.4322/bds.2022.e2884
  Issue No: Vol. 25, No. 1 (2022)
Replacement of maxillary lateral incisors with digitally manufactured
single tooth retained all-ceramic pontics: a clinical report
- Authors: Bahar Elter, Akın Aladağ, Muharrem Erhan Çömlekoğlu, Mine Dündar Çömlekoğlu
  Abstract: Several treatment options for replacement of anterior missing teeth exist, varying from no-prep (no preparation)
  adhesive restorations to implants. Patients’ biological, psychological, economic conditions or age have an effect on
  the treatment choice. It is important to perform the most conservative and beneficial treatment option considering
  the patient based factors. The aim of this case report was to demonstrate a single session, minimally invasive,
  cantilevered 2-unit all-ceramic restorations replacing anterior missing teeth combined with the advantages of
  CAD/CAM technology and materials.
  KEYWORDS
  CAD/CAM; Cantilever; Digital dentistry; Resin-bonded Fixed dental prostheses.
  PubDate: 2022-02-02
  DOI: 10.4322/bds.2022.e2856
  Issue No: Vol. 25, No. 1 (2022)
Color change, tooth sensitivity, aesthetic self-perceptions and quality of
life after at-home dental bleaching
- Authors: Larissa Cavalcante, Cynthia Bomgiovanni, Karinne Antunes, Mayra Cardoso, Eduardo Loureiro, Patricia Tannure, Luis Felipe Schneider
  Abstract: Objective: The aim of this study was to determine the color change, tooth sensitivity, aesthetic self-perception
  and quality of life after at-home dental bleaching. Material and Methods: Dental examination was performed
  and the initial color of the upper right incisor was recorded using a spectrophotometer (Easyshade Compact,
  Vita Zahnfabrik) and CIELab parameters. Individual vinyl trays were delivered to the patients (n=37) who were
  instructed to use the carbamide peroxide 16% (Total Blanc Home C16%, DFL) for 2 hours daily for 2 weeks.
  One-week after the end of treatment, measurements were repeated to calculate color (Eab, E00) and whiteness
  index (WID) variations. Oral Health Impact Profile (OHIP) and Psychosocial Impact of Dental Aesthetics
  Questionnaire (PIDAQ) questionnaires were applied before and 30 days after treatment. Tooth sensitivity was
  by checked by visual analogue scale (VAS). Spearnan’s correlation tests were used to verify the possible relation
  between color change parameters and sensitivity. The Wilcoxon test was applied for results obtained by OHIP and
  PIDAQ questionnaires. Results: Significant color change was observed after 14 days, regardless of the considered
  parameter. There was no significant correlation between sensitivity and color change. The dental self-confidence
  domain increased after bleaching (p = 0.029) and the psychological impact decreased (p = 0.001). Conclusion:
  At-home dental bleaching was effective on the overall whitening and the color change was not related to tooth
  sensitivity. The bleaching treatment had a positive impact on the quality of life, reducing psychological discomfort
  and improving dental self-confidence and aesthetic self-perception. KEYWORDS
  Aesthetics; Quality of life; Tooth whitening.
  PubDate: 2022-01-27
  DOI: 10.4322/bds.2022.e2739
  Issue No: Vol. 25, No. 1 (2022)
Esthetic patient satisfaction, marginal bone loss and peri-implant tissue
success in esthetic zone: systematic review
- Authors: Sherif Bushra, Lamiaa Khierla, Jylan El Guindy, Nadia Fahmy, Hani El Nahass
  Abstract: Increased patient demands for highly esthetic implant superstructure in the anterior esthetic zone has increased in
  the last decades. Moreover, the absence of periodontal ligament in implant supported prosthesis causes forces to
  be transferred without cushioning effect to the alveolar bone, resulting in increased marginal bone loss (MBL) and
  influence the heath of peri-implant tissue. Evaluate the available evidence on the effect implant superstructure and
  it consequences on patient satisfaction, MBL, bleeding on probing (BOP) and probing depth (PD). A protocol of
  electronic and hand research was performed for English based researches comparing implants inserted in the esthetic
  zone with all ceramic superstructure: “Will the use of different types of all ceramic superstructure show different
  esthetic patient satisfaction, marginal bone loss, bleeding on probing and probing depth' Thirteen publications
  from one thousand one hundred and sixteen research studies were included. This systematic review showed that all
  ceramic implant superstructure was a versatile treatment option with higher esthetic patient satisfaction and better
  color of peri-implant mucous especially in patients having thin biotype. On the other hand there wasn’t significant
  difference in MBL, PD and BOP compared to other conventional implant superstructure. More randomized controlled
  clinical trials with bigger samples are needed to confirm our findings. All ceramic implant superstructure is versatile
  and highly esthetic treatment option for implant placed in the anterior esthetic zone.
  KEYWORDS
  Esthetic; Patient satisfaction; Tissue success; Marginal bone loss; Superstructure.
  PubDate: 2022-01-27
  DOI: 10.4322/bds.2022.e2514
  Issue No: Vol. 25, No. 1 (2022)
Evaluation of desensitizing efficacy of nanohydroxyapatite on the
treatment of dentine hypersensitivity following ultrasonic scaling: a
randomized controlled trial
- Authors: Walla Alsen, Imad Barngkgei, Solayman Dayoub
  Abstract: Objective: This randomized controlled trial aimed to compare nanohydroxyapatite with fluoride on managing
  post ultrasonic scaling Dentine hypersensitivity (DH). Material and Methods: Thirty patients (aged 20-50
  years) with post ultrasonic-scaling DH were included in this study. The sample was randomly divided into three
  equal groups of 60 teeth each: the first group received nanohydroxyapatite material, the second group received
  fluoride material and the third group received sterile water as a placebo (controls). The materials were applied
  once for each patient. All patients were instructed to rate the level of pain before treatment, and after 1 hour, 24
  hours, 2 weeks and 1 month on the numerical rating scale (NRS). The Kruskal-Wallis test, Mann-Whitney tests,
  linear regression analysis were used for the statistical analysis. Significance level was set at 0.05. Results: Both
  nanohydroxyapatite and fluoride were successful in reducing pain associated with DH when compared with the
  placebo in subsequent follow-ups (p < 0.05). However, one-hour and one-day post application, nanohydroxyapatite
  could reduce hypersensitivity pain more effectively than fluoride (p < 0.05). Conclusion: Nanohydroxyapatite
  material was found to be significantly more effective in reducing the DH that followed ultrasonic scaling one-hour
  and one-day post application as compared to fluoride and sterile water. Both fluoride and nanohydroxyapatite
  had similar effect on DH after two-weeks and one-month after application.
  KEYWORDS
  Dentine hypersensitivity; Desensitizing agent; Fluoride; Nanohydroxyapatite; Ultrasonic scaling.
  PubDate: 2022-01-24
  DOI: 10.4322/bds.2022.e2737
  Issue No: Vol. 25, No. 1 (2022)
The relationship between prevalence of dental anxiety and dental caries:
cross-sectional study
- Authors: Benin Dikmen, Isik Saral, Musa Kazim Ucuncu, Esra Yildiz, Sevda Ozel Yildiz
  Abstract: Objective: This cross-sectional study aimed to assess level of DA among dental students in different grades
  and patients and to evaluate associations between DA and presence of dental caries. Material and Methods:
  Both four hundred all-grade dental students of Istanbul University and patients of Istanbul University Hospital
  were included in the study after random selection. Data regarding gender, oral hygiene habits, frequency of
  dental visits, and educational level were obtained via questionnaire. DA of participants were measured using
  both Modified Dental Anxiety Scale (MDAS) and Dental Fear Survey (DFS). The prevalence of caries was
  determined using International Caries Detection and Assessment System Criteria (ICDAS). Data were analyzed
  using Kruskal-Wallis, Mann-Whitney U and Chi square tests. Results: Female patients and female 4th-year
  students had significantly higher MDAS scores (p<0.05). First-year students had significantly higher MDAS and
  DFS scores than 4th and 5th-year students (p<0.05). Patients who had caries and brushed their teeth less than
  twice a day had significantly higher MDAS scores (p<0.05). Among 3rd-year students, individuals who visited
  dentist regularly had lower MDAS scores (p<0.05). Conclusion: DA among senior dental students was lower
  than that in younger students with an increase in knowledge about dentistry. Tooth brushing frequency and
  caries prevalence was associated with DA in patient group. No association was found between DA and frequency
  of dental visits, except in 3rd-year students.
  KEYWORDS
  Dental anxiety; Dental caries; Dental student; Prevalence.
  PubDate: 2022-01-24
  DOI: 10.4322/bds.2022.e2598
  Issue No: Vol. 25, No. 1 (2022)
Influence of photobiomodulation and tricalcium silicate-based cement on
viability and dentinogenic activity of cultured human dental pulp stem
cells
- Authors: Latifa Mohamed Abdelgawad, Mustafa Hasan, Dina Sabry, Marwa Abdelgwad
  Abstract: Objective: To assess the effect of application of Biodentine (BD), Photobiomodulation (PBM) using 810 nm diode
  laser and both on the proliferation and odontogenic differentiation of human dental pulp stem cells (HDPSCs).
  Material and Methods: HDPSCs were collected, isolated, and characterized and then divided into six groups:
  groups 1, control; groups 2, biodentine (BD); group 3, irradiation at 1 J/cm2 of 810-nm diode laser; group 4,
  irradiation at 1 J/cm2 and culture with BD; group 5, irradiation at 2 J/cm2, and group 6, irradiation at 2 J/cm2
  and culture with BD. Viability assay was measured through MTT assay and Alkaline phosphatase (ALP) enzyme
  activity and mRNA levels of RUNX2, collagen 1 (Col-1) and BMP2 were also assessed. Results: Photobiomodulation
  at 1 and 2 J/cm2 combined with biodentine significantly promoted HDPSCs proliferation (in MTT assay results)
  and odontogenic differentiation (through the gene expression of RUNX2, Col-1 and BMP2 levels (p < 0.05).
  Conclusion: Photobiomodulation at 2 J/cm2 combined with biodentine enhanced proliferation and odontogenic
  differentiation of cultured HDPSCs and thus could further be beneficial for dentin regeneration.
  KEYWORDS
  Human dental pulp stem cells (HDPSCs); PBM; BMP2; RUNX2; COL1.
  PubDate: 2022-01-18
  DOI: 10.4322/bds.2022.e2497
  Issue No: Vol. 25, No. 1 (2022)
Effectiveness of three different local routes of dexamethasone
administration on postoperative sequelae following mandibular third molar
surgery. A prospective randomised single-blind clinical study
- Authors: Mohamed Ihab Mosleh
  Abstract: Objective: The aim of this study was to provide evidence for comparing the effectiveness of three different routes
  of local administration of Dexamethasone on the postoperative pain, edema and trismus following surgical removal
  of impacted mandibular third molar. Material and Methods: Forty-five patients underwent surgical removal of
  impacted lower third molars and were randomly allocated postoperatively into 3 groups: 8 mg of dexamethasone
  injected into the submucosa of the vestibule near the surgical site (group I), 8 mg of dexamethasone injected into
  the pterygomandibular space (group II) and 10 mg of dexamethasone powder applied to the extraction site, after
  bleeding control (group III). Facial swelling and maximal interincisal opening were measured at preoperatively.
  Pain was measured by the patient response to a visual analogue scale. Pain perception, Facial edema and trismus
  were evaluated for one week postoperatively. Results: There was no significant difference between the three
  groups concerning pain after 1, 2, 5, 7 days of follow up. However, group II showed less pain at 3 and 4 days.
  The difference between edema measurements was not significant in the three groups at 1, 5, 7 days, though in
  group I and II edema subsided from day 2. As for trismus, group I and III showed statistically significant lower
  maximum interincisal opening measurement than group II after two days. Conclusion: Local administration
  of Dexamethasone through three different routes is beneficial in decreasing postoperative sequelae following
  third molar surgery. Pterygomandibular space injection of Dexamethasone resulted in earlier resolution of pain,
  and less facial edema and trismus at the second postoperative day compared to the submucosal injection and
  transalveolar application. However, at one week the difference in measurements of the three variables between
  the groups was not significant.
  KEYWORDS
  Dexamethasone; Local routes of administration; Mandibular third molar; Surgery.
  PubDate: 2022-01-12
  DOI: 10.4322/bds.2022.e2719
  Issue No: Vol. 25, No. 1 (2022)
Influence of titanium nanotubular surfaces, produced by anodization, on
the behavior of osteogenic cells: in vitro evaluation
- Authors: Juliani Caroline Ribeiro de Araújo, Lidiane Lopes do Espirito Santo, Sandra Giacomin Schneider, Gabriela de Fátima Santana- Melo, Isabel Chaves Silva Carvalho, Denise Richter Franzini, Marianne Spalding, Luana Marotta Reis de Vasconcellos
  Abstract: Objective: The objective of this study was to evaluate in vitro the influence of the anodized surface of Ti35Nb7Zr
  alloy on the behavior of osteogenic cells, for future application in biomedical implants. Material and Methods:
  For the development of this research, samples of commercially pure titanium (TiCp) and samples of Ti35Nb7Zr
  alloy were anodized, both were characterized by scanning electron microscopy (SEM) and were plated afterwards
  with human osteoblast-like cells (MG63 line) (2 x 104). Cell adhesion, cytotoxicity test, formation of mineralization
  nodules and a comet assay were also performed in different periods. The bottom of the plate was used as a
  control, without a sample. Results: SEM analysis showed that the topography of both samples presented surfaces
  covered by nanotubes. Cellular morphology exhibited spreading in both samples proposing an intimate cellmaterial
  liaison. After 3 days, the Ti35Nb7Zr group exhibited greater cell viability than the TiCp group (p<0.01).
  Regarding calcium content, there was no statistical difference between the anodized groups, but there was a
  difference between the experimental groups and the control group (p<0.01). In the comet assay, the percentage
  of DNA in the comet tail did not exhibit any significant difference (p>0.05) among the groups in the evaluated
  periods. Conclusion: It was concluded that this process of anodization was efficient to form nanotubes, as well
  as promote a positive influence on the behavior of osteogenic cells without promoting cell damage.
  KEYWORDS
  Nanotopography; Osteoblast; Titanium alloy; Titanium implants.
  PubDate: 2022-01-07
  DOI: 10.4322/bds.2022.e2528
  Issue No: Vol. 25, No. 1 (2022)
Lesion sterilization and tissue repair of primary molar to the eruption of
its permanent successor: a case report
- Authors: Claudia Paulo Moreira, Regina Maura Coli Siegl, Tamara Kerber Tedesco, Thais Gimenez, Isabela Floriano, Jose Carlos Imparato
  Abstract: The aim of this study is to report a clinical case of lesion sterilization and tissue repair in primary molar, with
  follow-up until the eruption of the permanent successor. A 6-year-old male patient came to the Pediatric Dentistry
  Clinic at Faculdade Sao Leopoldo Mandic in Campinas-SP, with his mother, complaining of “caries in several
  teeth”. On clinical examination, it was found that tooth 85 had a severe carious lesion with pulp involvement
  and fistula. Radiographically, radiolucency was observed in the furcation region. It was then decided to perform
  lesion sterilization and tissue repair of the tooth with CTZ paste. After 6 months of the procedure, the tooth
  was clinically and radiographically normal and after 3 years it was observed that tooth 85 was in complete
  root resorption. After extraction, the permanent successor erupted naturally without clinical or radiographic
  alterations. It can be concluded that the present clinical case demonstrated success in the use of CTZ paste in the
  lesion sterilization and tissue repair of primary molars, until the complete eruption of the permanent successor.
  KEYWORDS
  Primary tooth; Pulpectomy; Pediatric dentistry; Endodontics; Case report.
  PubDate: 2022-01-07
  DOI: 10.4322/bds.2022.e2903
  Issue No: Vol. 25, No. 1 (2022)
Impact of different scan strategies and implant angulation on impression
accuracy of full arch multiple implant: an in vitro study
- Authors: Danilo Lopes, Roger Nishyama, Washington Steagall Jr., Regina Tamaki, Pedro Tortamano Neto
  Abstract: Objective: to evaluate the impact of three different scan strategies and implant angulation on impression accuracy
  of an intraoral scanner for full-arch multiple implant scan. Material and Method: A maxillary edentulous model
  with six implant analogs served as a reference model. The four anterior analogs were positioned parallel to
  each other, the distal right and the distal left was placed with an angulation of 15o and 20o, respectively. Thirty
  impression were performed using an intraoral scanner (CEREC Primescan). The master cast was digitalized with
  an industrial reference scanner (ATOS Core 80). All scans were converted to standard tessellation language (STL),
  superimposed on the reference scan with a 3d inspection software (GOM Inspect Professional 2019) and then
  analyzed. Results: All linear distances presented equivalence [p<0.01] to those found on the reference scan for
  all scan strategies. All scan strategies presented a tendency of negative means for linear distances except for d4
  in strategy C. All angular distances did not present equivalence [p=0.05] to those found on the reference scan.
  Significant 3D deviations [p<0.05] were found between strategy B (0.02 ± 0.01) and C (0.05 ± 0.04) for d1.
  In all others linear and angular distances no statistically significant difference was found between strategies A,
  B and C. Conclusions: There was no statistically significant difference between strategies A, B and C except for
  d1 in strategy B and C; Implant angulation did not affect the accuracy of the CEREC Primescan IOS.
  KEYWORDS
  Precision; Trueness; Edentulous jaw; Dental implant; Dental impression technique.
  PubDate: 2022-01-03
  DOI: 10.4322/bds.2022.e3006
  Issue No: Vol. 25, No. 1 (2022)
Effect of immediate dentin sealing and temporary cement removal on bond
strength of resin cements to dentin
- Authors: Luís Felipe Guilard, Kiara Serafini Dapieve, João Carlos Giordani, Alexandre Henrique Susin, Luiz Felipe Valandro, Marília Pivetta Rippe
  Abstract: Objective: To evaluate the influence of immediate dentin sealing (IDS) and mechanical methods for removing the
  temporary cement on the bond strength between dentin and resin cements. Material and Methods: Bovine incisors
  were ground until dentin exposure and divided according to two factors: “dentin surface treatment”’ - cleaning
  with manual dental excavator (DE), with Robinson bristle brush and pumice paste (PP) or IDS application with
  Single Bond 2 (IDS/SB+PP) or Single Bond Universal (IDS/SBU+PP) plus cleaning with PP; and “resin cement”
  - Self-adhesive (RelyX U200) or conventional cement with self-etching adhesive (Multilink Automix). Simulating
  provisional restoration, acrylic resin plates were cemented onto the dentin surface (with or without IDS) with a
  non-eugenol temporary cement, and stored in distilled water (37 ºC; 7 days). The acrylic plates were removed, the
  dentin surface was cleaned (PP or DE), and starch tubes were positioned on the dentin where the resin cements
  were applied. After 24 h, the specimens were submitted to a microshear test (wire-loop method). Results: Twoway
  analysis of variance showed statistically significant influence of dentine surface treatments (p< 0.001) and
  resin cement (p= 0.001) in the bond strength values. The IDS/SBU+PP/U200 (7.24 MPa) and IDS/SBU+PP/
  MULTI (6.40 MPa) groups presented higher values when compared to cleaning with DE (DE/U200= 4.60 MPa;
  DE/MULTI= 1.45 MPa) and PP (PP/U200= 3.74 MPa; PP/MULTI= 3.14 MPa) Statistical difference was also
  found between the cements when dental excavator treatment was used (RelyX U200 > Multilink Automix). The
  IDS/SBU+PP protocol presented a higher percentage of cohesive failures. The micrographs showed differences
  in dentin surface characteristics among the groups. Conclusion: Immediate dentin sealing increased the bond
  strength of the resin cements to dentin compared to mechanical cleaning only, regardless the resin cement. KEYWORDS
  Cleaning; Dentin sealing; Provisional cement; Resin cement.
  PubDate: 2022-01-03
  DOI: 10.4322/bds.2022.e2729
  Issue No: Vol. 25, No. 1 (2022)