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European Research Journal
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This is an Open Access Journal

Open Access journal
ISSN (Online) 2149-3189
Published by DergiPark

[220 journals]

MicroRNA-33a levels do not correlate with the expression of its host gene
SREBF2 and its isoforms in prostate cancer cell lines
- Authors: Ömer Faruk Karataş; Michael Ittmann
  Abstract: Objectives. Prostate canceris currently the most frequently diagnosed malignantneoplasm and the second leading cause of cancer relatedmortality in men over the age of 50 years in the developed countries. MicroRNA-33a(miR-33a), localized within the intron 16 of SREBF2, has been reported to have tumor suppressive properties insome cancers including prostate cancer, whereas its host gene, SREBF2, has beenshown to be elevated in prostate cancer and to act as an oncogene. Due to theparadoxical expression of an oncogene and a tumor suppressor from a singlegenetic locus, there is a need for evaluation of miR-33a and SREBF2 expressionstatus in prostate cancer cells to help understanding their roles in prostatecarcinogenesis. Methods. In this study, we aimed at investigating the linkbetween the expressions of miR-33a and its host gene SREBF2 and its isoforms inprostate cancer cell lines using quantitative real time PCR. We evaluated therelative expression levels with using 2- ΔΔCT method and tested thecorrelations of microRNA and gene expressions with Pearson’s Correlation testusing GraphPad Prism 6. Results. Our results demonstratedvariable expression levels for SREBF2 mRNA and miR-33a expression levels inprostate cancer cell lines, with some decreased, some increased and someunchanged. Further analysis showed a strong correlation among expressions ofSREBF2 isoforms though we could not find a significant association betweenlevels of SREBF2 isoforms and miR-33a expression. Conclusion. This datasuggest possible posttranscriptional regulation of miR-33a expression inprostate cancer.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
The effect of obesity on functional capacity, anxiety and daily life
activities in patients with coronary artery disease and phase II cardiac
rehabilitation
- Authors: Meliha Kasapoğlu Aksoy; İlknur Aykurt Karlıbel, Hasan Arı, Lale Altan
  Abstract: Objective. The aim of our study was to investigate the effect ofcardiac rehabilitation (CR) program on obese and non-obese patients withcoronary artery disease. Methods. The records of 60coronary artery disease patients aged between 18-75 years, who were included inCR were evaluated. Of the study subjects, 20 had undergone coronary arterybypass grafting (CABG) and 40 had undergone percutaneous coronary intervention(PCI). The patients weredivided into two groups by body mass index (BMI), with Group I being non-obese(BMI < 30 kg/m2) and Group 2 being obese (BMI ≥ 30 kg/m2).The effect of 30 session CR on the two groups were evaluated with 6-min walktest (6MWT), Short Form-36 (SF-36) and Beck Anxiety Inventory (BAI). Results. Baseline characteristicsof the group 1 (39 patients) and group 2 (21 patients) were similar.Statistically significant improvement was detected in group 1 and group 2patients by CR program in 6MWT, BAI and SF-36 parameters (p < 0.05). 6MWT, BAI and SF-36 parameters changes by cardiacrehabilitation were compared between the two groups. According to thecomparison 6MWT (group 1; 60 (20-183) vs group 2; 34 (15-180), p = 0.012) and MET (group 1; 1.44 ± 0.56vs group 2; 1.09 ± 0.41, p = 0.015changes were significantly higher in group 1 than in group 2. However, thechanges of BAI and SF-36 parameters were similar in two groups (p > 0.05). Conclusion. CR was foundto be effective and safe in terms of functional capacity, daily life activitiesand anxiety in both obese and non-obese patients. Functional capacity gain inthe obese group was less than non-obese patients.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Magnetic resonance imaging findings of a patient with bilateral
submandibular gland aplasia
- Authors: Ender Alkan; Turgay Kara, Zeynep Öztürk İnal
  Abstract: Aplasia of the major salivary glands are very rare,and commonly occurs at the parotid gland. Patients are usually asymptomatic anddiagnosed incidentally. But dry mouth, difficulty in swallowing, dentalproblems may be seen. Also mass complaints may occur due to compensatoryhypertrophy of the other major salivary glands. Herein, we presented themagnetic resonance imaging findings of a very rare case of bilateralsubmandibular gland aplasia with compensatory hypertrophy of sublingual glands.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Sensitivity of localization studies performed by various radiologists in
the evaluation of parathyroid lesions
- Authors: Pınar Şişman; Özen Öz Gül, Soner Cander, Ahmet Bilgehan Şahin, Çoşkun Demirtaş, Aybüke Muti, Onur Elbasan, Özlem Saraydaroğlu, Erdinç Ertürk
  Abstract: Objectives. Preoperative imaging studies are commonly used in thediagnosis of primary hyperparathyroidism to increase the success rate ofsurgery. In the present study, we aimed to correlate surgical outcomes with thesensitivity of localization studies that were performed by various radiologists. Methods. Onehundred eighty-nine patients with preoperative diagnosis primary hyperparathyroidismwere included. A total of 174 patients in whom hypercalcemia had been cured byparathyroidectomy, were evaluated retrospectively. In total, 184 lesions wereexcised from these 174 patients. Ultrasonography (USG) and technetium-99m-methoxy isobutyl isonitrile (99mTc-MIBI) imagingyielded correctly localized lesion in 74 and 108 patients, respectively. Results.The specificity of USG and 99mTc-MIBI imaging were similar (95.9%and 95.0%, respectively). However, the sensitivity was not satisfactory (45.9%for USG, 62.4% for 99mTc-MIBI). The gland size was not significantfor the rate of lesion detection by 99mTc-MIBI scan or USG.Significant differences were not observed between the preoperative serumparathormone, serum calcium or 24-hour urine calcium excretion levels and thesuccess rate of localization with either USG or 99mTc-MIBI. Conclusions.Radiologist experience in ultrasonographic parathyroid imaging was found toaffect sensitivity. Therefore, surgeons, radiologists and endocrinologists thatperform ultrasonographic evaluation should have extensive experience.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Effect of functional endoscopic sinus surgery on asthma control with nasal
polyposis and chronic rhinosinusitis
- Authors: Önder Akdeniz; Seyhan Dülger, Dilber Yılmaz Durmaz, Özlem Şengören Dikiş, Tekin Yıldız
  Abstract: Objective.The purpose of this study is to examine the effect of endoscopic sinus surgeonson the control of disease in asthma combined with chronic rhinosinusitis. Methods.Twenty-three asthma patients with chronic rhinosinusitis were evaluatedretrospectively. They were assessed with asthma control test and pulmonaryfunction tests. Subjective nasal obstruction assessment was done according tothe nasal obstruction symptoms evaluation (NOSE) scale. Other subjectivesinonasal complaints were classified using visual analogue scale (VAS). At postoperativethird month, pulmonary function tests, asthma control test, NOSE and VAS werereevaluated. Results. According to asthma control test, number of patientsunder control, partial controlled and uncontrolled were 2/6/15 preoperatively and14/4/5 postoperatively, respectively. There was permanent or partialimprovoment in fullness in the nose sniffle of 95.7% according to NOSE. Themost remarkable permanent improvement in the symptoms were seen in the feelingof pressure in the face of 75%, smell disorders of 66.7% according to sinonasalvisual analogue scale assessment. NOSE and VAS values were significantlyimproved postoperatively (p <0.05). Conclusions. Uncontrolled asthma associated with chronicrinosinusitis can benefit from functional endoscopic sinus surgery in asthmacontrol level, nasal blockage symptoms and other sinonasal complaints.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
In vitro transfection potential of fluorinated G5 PAMAM dendrimers for
miRNA delivery to MRC-5 cells
- Authors: Ali Öztuna; Hasan Nazır
  Abstract: Objectives. MicroRNAs (miRNAs) are involved in the regulation ofmost biological processes and also contribute to many types of disease.Fibroblast cells, such as MRC-5, are often used in biological researchesutilizing cell transfection methods due to their difficult to transfect nature.Cells can be genetically engineered by using viral and non-viral methods.Poly(amidoamine) (PAMAM) dendrimers are very promising alternative as adelivery vehicle due to their well-definedcharacteristics. In this study, in vitrotransfection potential of cystamine core generation five (G5) PAMAM dendrimersfluorinated with 2,3,4,5,6-pentafluorobenzoic acid (PFB) and pentafluoropropionicacid (PFP) for miRNA delivery to MRC-5 cells was examined. Methods. Spectroscopictechniques were used in the characterization of the prepared dendrimers. miRNAbinding and condensation capability of dendrimers was examined by gelretardation assay. Characterization of dendriplexes was made by zeta potential,particle size measurements and transmission electron microscopy. Transfectionefficiencies of the dendriplexes were determined by flow cytometry andintracytoplasmic distribution of the dendriplexes was shown by laser scanningconfocal microscopy. Also, quantitative structure-activity relationship and moleculardocking calculations were used to be able to discuss transfection efficienciesof the dendriplexes into the cell. Results. While high level of viabilityon MRC-5 cells was observed for dendriplexes prepared with PFB and PFP,transfection efficiency with PFP was higher than PFB. Transfection efficiencydifference between these two compounds was attributed to their molecularstructures. Conclusions. Obtained results hold promise for the usage ofthese compounds as a transfection reagent at MRC-5 cells. Further studies areneeded to support these findings.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
The frequency of toll-like receptor 4 gene polymorphism in ankylosing
spondylitis and its relationship between disease activity
- Authors: Betül Sargın; Ayla Akbal, Hatice Resorlu, Yılmaz Savaş, Coşkun Zateri, Fatma Sılan, Öztürk Özdemir
  Abstract: Objectives. We aimed to evaluate the frequency of toll-likereceptor 4 (TLR4) gene polymorphism and its relationship between diseaseactivity in patients with ankylosing spondylitis (AS). Methods. Forty-one AS patients (25 male/16female) fullfilling the 1984 Modified New York Criteria and 41healthycontrols (25 male/16female) were included in this study. Disease activity of the AS patients wasassesed by Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). The TLR4 gene polymorphism of AS patients and healthy controls were analyzed byReal-Time Polymerase Chain Reaction (PCR) System. Results.Three (7.3%) patients with AS had TLR4 gene polymorphism compared with healthycontrols (0/41; 0%). Two of these patients had heterozygous mutation and onehad homozygous mutation. Significant correlation was not found between TLR4gene polymorphism and BASDAI score (p> 0.05). Conclusions. In our study, TLR4gene polymorphism was higher in patients with AS compared with control group. But,this polymorphism was not associated with disease activity, erythrocyte sedimentation rate and C-reactive proteinlevels.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Personnel radiation dose assessment using a novel dosimeter in the
department of radiology and dentistry in a medical facility in Delta
State, South-South Nigeria: Our experience in the last 4 years
- Authors: Akintayo Daniel Omojola; Michael Onoride Akpochafor, Samuel Olaolu Adeneye, Ukeme Pius Aniekop, Margaret Idongesit Anizor, Mary-Ann Etim Ekpo, Chibuzor Bede Madu
  Abstract: Objectives. Large percentagesof X-ray facilities in Nigeria do not use radiation monitoring device; a fewpercentage that use them do not evaluate or carryout out assessment programs toascertain the detriment to occupationally exposed workers. This study was aimedat evaluating dose reports from 2013 to 2016 for personnel who operate radiation facilities and those that workwithin radiation field during certain X-ray procedures/examinations in thedepartment of radiology and dentistry respectively; to ascertain if there iscorrelation between personnel dose and workload in both department and todetermine if dose records are within acceptable limit recommended by theinternational atomic energy agency (IAEA) safety series. Methods. Direct ion storage (DIS) dosimeter wasused for a total of 35 occupationally exposed personnel who work in thedepartment of radiology and dentistry. The DIS dosimeter was read every twomonths and results were authomatically saved on the instadoseTMplatform. Results. The mean (total) dose in radiology department for thefirst, second, third and fourth year was 0.17 ± 0.08 (3.52) mSv, 0.08 ± 0.03(0.77) mSv, 0.07 ± 0.04 (0.72) mSv and 0.07 ± 0.05 (0.55) mSv and in Dentistrywas 0.08 ± 0.02 (0.73) mSv, 0.05 ± 0.02 (0.42) mSv, 0.05 ± 0.02 (0.24) mSv and0.07 ± 0.04 (0.34) mSv; respectively. There was significant difference in meanpersonnel dose from 2013-2016 in Radiology (p= 0.028) and in Dentistry Department (p= 0.004). Correlation of workload and personnel dose inRadiology (p = 0.240) and DentistryDepartment (p = 0.765) wasn’tsignificant. There was nocorrelation in mean dose between both department (p = 0.256). Conclusion. Overall mean dose inboth department for occupationally exposed personnel werebelow IAEA annual dose limit of 20 mSv averaged over a period of 5consecutive years. Dose reports ofpersonnel in both department reduced as the year progressed due to radiationsafety awareness.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
How to diagnose neuropathy in diabetes mellitus'
- Authors: Abdulkadir Koçer
  Abstract: Diabetic neuropathy is a common problem and canpresent various clinical presentations such as cranial neuropathy,radiculopathy, plexopathy, mononeuropathy, polyneuropathy and autonomicneuropathy. Clinical evaluation and use of scoring systems for the evaluation ofdiabetic polyneuropathy is an important step to get correct diagnosis. Newinformations about the diagnosis of diabetic neuropathy continue to emerge,which will lead to correct diagnosis and treatment as well. Diabetic length-dependentsensorimotor polyneuropathy (DSPN) and carpal tunnel syndrome are the mostcommon seen problems. While acuteand painful situations with motor weakness are mostly transient, sensory fibresare predominantly involved in chronic ones. Nerve conduction studies areneeded to confirm diagnosis of any type of diabetic neuropathy, but they arenormal in cases with small fiber involvement. The early diagnosis is crucial because it is well-known thatsubclinical diabetic neuropathy may be reversed or significantly improved withdiabetes control. Because neuropathy at the stagein which only small fibers are affected can be reversed, it is important todiagnose DSPN in these stages. Skin biopsy taken from the dermatomal area ofsural nerve, laser-doppler-imagerflare technique, corneal confocal microscopy are used to assess small fibre dysfunction. The aim ofthe present review was to evaluate evidence-based diagnosis for any type ofneuropathy seen in the patients with diabetes mellitus.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
An arteriovenous malformation as a rare cause of sciatic neuropathy: a
case report
- Authors: Ezgi Akar; Mustafa Akgün, Mehmet Ufuk Akmil
  Abstract: Sciatica is characterized by pain and discomfortoccurring frequently with impingement at the level of spinal nerve along theregions innervated by the sciatic nerve. Compression and irritation of thenerve often occurs with a spinal cause such as lumbar disc hernia or spinalstenosis. Compression of the nerve by an arteriovenousmalformation is very rare among thecauses leading to nondiscogenic sciatic neuropathy. Herein, we reported ourcase with clinically and electrophysiologically typical sciatic neuropathy andtreated by us surgically. To our knowlodge, cases with arteriovenous malformation -caused sciatica werelimited in the literature review. Electromyography should be performed to exclude the nervecompression due to rare causes such as vascular causes which may lead tosciatic neuropathy in patients with sciatic distribution symptoms and signs,after initial negative spine imaging.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Metachronous infiltration of bone marrow due to breast cancer and multiple
myeloma
- Authors: Ahmet Peker; Hakan Yarkıcı, Mehmet Can Uğur, Harun Akar, Cengiz Ceylan, Dudu Solakoğlu Kahraman
  Abstract: Multiple primary tumors are rarely seen in clinicalpractice. Cases of metastatic breast cancer associated with multiple myelomawere rare in the literature. It is believed that simultaneously observed bonemarrow involvement due to breast cancer and multiple myeloma is sporadic. Wepresent a 49-year-old female patient with metastatic breast cancer who developedmultiple myeloma during breast cancer treatment.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Pedunculated liver hemangioma mimicking stomach neoplasm
- Authors: Sonay Aydın; Erdem Fatihoğlu, Elif Ergün, Pınar Nercis Koşar
  Abstract: Hepatic hemangiomas are the most frequently seenbenign liver mass. However, exophytic hepatic hemangiomas, particularlypedunculated ones are very rare. They have various appearances that make thediagnosis difficult. We report a pedunculated hemangioma case which wasmisdiagnosed as gastrointestinal stromal tumor on computerized tomography scan.Magnetic resonance imaging revealed that the tumor was a hepatic hemangiomawith a long peduncle originating from the right edge of the liver, extendinginto perigastric area. Hepatic hemangioma with a long peduncle can be mistakenwith other abdominal masses. Remembering this entity and knowing its imagingcharacteristics are the keys to diagnosis.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Left atrial myxoma combined with coronary artery disease in an elderly
patient
- Authors: Gündüz Yümün; Selami Gürkan, Okan Donbaloğlu
  Abstract: Cardiac myxomaassociated with coronary artery disease is a rare pathology in an elderlypatient. Herein we present an 81-year-old woman undergoing simultaneous surgicaltreatment for left atrial myxoma combined with coronary artery disease. Thepostoperative course of the patient was uneventful. The initial removal of massshould be performed to prevent systemic embolization of tumor fragments.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030
Borderline Brenner tumor with a concomitant mucinous tumor of the ovary: a
case report
- Authors: Hülya Ayık Aydın; Zeynep Bayramoğlu, Armağan Aydın, Hadice Elif Pestereli, Tayup Şimşek
  Abstract: Brennertumors (BTs) are rare ovarian tumors which are a part of the epithelial stromaltumor group of ovarian neoplasms. Most of the BTs are benign and usuallyasymptomatic. BTs stand for the 1.4-2.5% of ovariantumors. Of the BTs, not more than 2% exhibit characteristics of borderline (ormalignant) BTs. Borderline BTs are usually bigger than benign BTs andindividuals typically represent with symptoms related with an unilateralovarian mass. BTs are often related with mucinous cystadenomas or seldomlycystadenocarcinomas. We present a case of borderline BT associated with aconcomitant mucinous ovarian tumor.
  PubDate: Wed, 04 Apr 2018 00:00:00 +030